Mirnas differentially expressed in lymph nodes from cancer patients

ABSTRACT

The present invention provides diagnostics and/or prognostic methods by identifying miRNAs that are differentially expressed or mis-regulated in various states of diseased, normal, cancerous, and/or abnormal tissues, including but not limited to cervix, skin, colon, breast, bladder, esophagus, liver, ovary, prostate, lung, pancreas, thyroid, kidney, stomach, testicle, uterus, spleen, tongue, brain, thymus, trachea and/or small intestine. In certain apsects, differential miRNA expression is initially determined by comparing miRNA expression between a normal tissue and cancer negative lymph node (LNneg), a normal tissue and a cancer positive lymph node (LNpos), a cancerous tissue and LNneg and/or LNpos, LNneg and LNpos. The methods of the invention are used to identify the presence or absence of non-lymphoid cells from other organs or tissues, and/or metastatic cancer cells present in lymph nodes.

This application claims priority to U.S. Provisional Patent ApplicationSer. No. 61/027,198 filed Feb. 8, 2008, which is incorporated herein byreference in its entirety.

BACKGROUND OF THE INVENTION

I. Field of the Invention

The present invention relates generally to the fields of molecularbiology and oncology. More particularly, it concerns methods andcompositions involving microRNA (miRNAs) molecules and cancer prognosisand staging. Certain aspects of the invention include applications formiRNAs in diagnosis and staging of various cancers and in patientmonitoring.

II. Background

Cancer remains a serious public health problem in the United States andother developed countries. Currently, one in four deaths in the UnitedStates is due to cancer (Jemal et al., 2007). Cancers of thelung/bronchus, prostate, and colon/rectum in men, and cancers of thelung/bronchus, breast, and colon/rectum in women are the most commonfatal cancers in the US, accounting for half of all cancer deaths (Jemalet al., 2007). Only a handful of treatments are available for specifictypes of cancer, and these provide no guarantee of success. To be mosteffective, cancer treatment requires not only an early detection of themalignancy, but a reliable assessment of the severity of the malignancy.

Cancer staging describes the extent or severity of an individual'scancer based on characteristics of the original (primary) tumor and theextent, if any, of cancer spread in the body. Clinical trials havecompared patients of similar stages to predict the probability of cancerrecurrence after surgical removal and to choose appropriate clinicalmanagement and surveillance.

In the United States, the American Joint Committee on Cancer (AJCC)provides criteria for staging based on the TNM staging system (Greene,2002). The TNM system was developed by the International Union AgainstCancer (UICC) and the American Joint Committee on Cancer (AJCC) andattempts to provide a uniform stratification of patients that allows forthe comparison of patients in clinical studies and for determiningoptimal treatment and survival rates. Common elements considered in theTNM and in most other staging systems include location of the primarytumor, tumor size and number of tumors (T), lymph node involvement(spread of cancer into lymph nodes) (N), cell type and tumor grade (howclosely the cancer cells resemble normal tissue), and presence orabsence of cancer metastasis (M). TNM criteria are different for eachanatomic cancer site. Cancer staging criteria are modified and updatedover time, as scientists learn more about individual cancer types andidentify which aspects of the system represent accurate predictors fordisease recurrence and patient survival.

For many cancers, lymph node involvement, or metastasis to lymph nodes,has become recognized as a strong predictor of disease recurrence andpatient survival. Metastatic deposits visible to the naked eye (>2 mm)are used to denote whether a lymph node is positive (Greene, 2002).Currently, metastatic cancer deposits <2 mm in size that are found in alymph node are referred to as micrometastatic lesions or micrometastaticdisease (MMD). However, under current staging systems, lymph nodes withMMD remain classified as negative (N0) for cancer metastasis. MMD iscurrently only recognized in the staging of breast cancer, due toextenisve evaluations of the prognostic significance of sentinel lymphnodes from breast cancer patients.

In general, current methods for assessing lymph node involvement,especially those for evaluating the presence of MMD, have significantlimitations. Classifying a tumor as metastatic is a coordinated effortamong a pathologist, a radiologist, a surgeon, and an oncologist.Microscopic examination of lymph nodes, typically performed by apathologist, is essential for proper cancer staging. Only a very smallfraction of a lymph node (approximately 0.1-0.2%), is evaluatedmicroscopically for metastatic cancer cells by a pathologist.Immunohistochemical (IHC) staining can increase the sensitivity bysubjecting an additional small fraction of the lymph node to a morespecific assay. Likewise, tissue sectioning at additional levels of thelymph node can increase the percentage of the lymph node that isexamined, but this procedure is used infrequently and most commonly forsentinel lymph node evaluation. Furthermore, protocols for lymph nodeevaluation are not standardized among pathologists, and no currentprotocol allows for microscopic examination of more than 10% of a lymphnode (Coello et al, 2004; Hughes et al., 2006; Ferris et al., 2005; Xiet al., 2005; Xi et al., 2006; Takeuchi et al., 2004; Scoggins et al.,2006; D'Cunha et al., 2005; Gillanders et al., 2004; Schurr et al.,2006; Houvenaeghel et al., 2006; Kammula et al., 2004). In addition, atleast one group has reported alarming discordance in histologicinterpretations by different pathologists (Roberts et al., 2003). As aresult of these limitations, 25% to 35% of cancer patients, who arelymph node-negative by the present state of the art examination, willexperience a recurrence of their cancer. It is believed that a majorcontributing factor to missed metastases is the presence in the lymphnode of occult metastasis (i.e., not readily apparent or hidden) and/orMMD that is not detected by the present state of the art examination.The current invention overcomes these problems in the art by describingquantitative molecular methods and novel molecular markers (microRNAs)(Lagos-Quintana et al., 2001; Lau et al., 2001; Lee and Ambros, 2001)for detection of metastatic cancer in lymph nodes.

microRNAs (miRNAs) are short RNA molecules (17-24 nucleotides in length)that arise from longer precursors, which are transcribed fromnon-protein-encoding genes. See review of Carrington et al (2003). Theprecursors are processed by cellular proteins to generate the shortdouble-stranded miRNA. One of the miRNA strands is incorporated into acomplex of proteins and miRNA called the RNA-induced silencing complex(RISC). The miRNA guides the RISC complex to a target mRNA, which isthen cleaved or translationally silenced, depending on the degree ofsequence complementarity of the miRNA to its target mRNA (Bagga et al,2005; Lim et al., 2005). Recent studies have shown that expressionlevels of numerous miRNAs are associated specifically with variouscancers (reviewed in Esquela-Kerscher and Slack, 2006; Calin and Croce,2006). miRNAs have also been implicated in regulating cell growth, cellproliferation, and cell and tissue differentiation—cellular processesthat are associated with the development of cancer. The presentinvention advances the current art for cancer staging by describing theuse of novel miRNA markers and combinations of miRNA markers for thedetection of specific cancer metastases to lymph nodes.

SUMMARY OF THE INVENTION

Embodiments of the present invention provides additional prognosticand/or diagnostics methods by identifying miRNAs that are differentiallyexpressed or mis-regulated in various states of diseased, normal,cancerous, and/or abnormal tissues, including but not limited to lymphnode (LN), cervix, skin, colon, breast, bladder, esophagus, liver,ovary, prostate, lung, pancreas, thyroid, kidney, stomach, testicle,uterus, spleen, tongue, brain, thymus, trachea and/or small intestine.As referred to herein, a tissue is a biological tissue. Biologicaltissue is typically defined as a collection of interconnected cells thatperform a similar function within an organism or collectively form anorgan. One of skill in the art can readily determine what part of thebody or organ a particular tissue designation refers to. In certainapsects, differential miRNA expression is initially determined bycomparing miRNA expression between a normal tissue and cancer negativelymph node (LNneg), a normal tissue and a cancer positive lymph node(LNpos), a cancerous tissue and LNneg and/or LNpos, LNneg and LNpos. Infurther aspects, the methods of the invention is used to identify thepresence or absence of ectopic cells from other organs or tissues,and/or metastatic cancer cells present in lymph nodes (LN), which aredetectable by the described methods. Lymph nodes are components of thelymphatic system. They are nodes that typically contain white bloodcells and are found throughout the body functioning as filters or trapsfor foreign particles. Further, the invention describes a method fordiagnosing diseased, normal, cancerous, abnormal tissues and/ordissemination of cancer cells based on determining levels (increased ordecreased) of selected miRNAs in patient-derived samples, particularlylymph nodes (e.g., sentinel, proximal, and/or distal LN). Samplesobtained and/or analyzed from patients, including but not limited topatients that have had, have or are suspected of having a cancer of thecervix, skin, colon, breast, bladder, esophagus, liver, ovary, prostate,lung, pancreas, thyroid, kidney, stomach, testicle, uterus, spleen,tongue, brain, thymus, trachea and/or small intestine or otherhyperproliferative or cancerous condition.

In some embodiments, it may be useful to know whether a cell expresses aparticular miRNA or set of miRNAs endogenously or whether suchexpression is affected under particular conditions or when it is in aparticular disease state. Thus, in some embodiments of the invention,methods include assaying one or more cell or sample containing a cellfor the presence of one or more miRNA. Consequently, in someembodiments, methods include a step of generating a miRNA profile forone or more samples. The term “miRNA profile” refers to a set of dataregarding the expression pattern for a plurality of miRNAs in asample(s); it is contemplated that the miRNA profile can be obtained,using for example, nucleic acid amplification or hybridizationtechniques well known to one of ordinary skill in the art.

In some embodiments of the invention, a miRNA profile is generated bysteps that include: (a) labeling miRNA in the sample; (b) hybridizingmiRNA to a number of probes, or amplifying a number of miRNA, and (c)determining miRNA hybridization to the probes or detecting miRNAamplification products, wherein a miRNA profile is generated. See U.S.Provisional Patent Application 60/575,743 and the U.S. ProvisionalPatent Application 60/649,584, and U.S. patent application Ser. No.11/141,707, all of which are hereby incorporated by reference.

Methods of the invention involve prognosing and/or diagnosing a patientbased on a miRNA expression profile in lymph node(s). In certainembodiments, the elevation or reduction in the level of expression of aparticular miRNA or set of miRNA in a sample is correlated with adisease or normal state compared to the expression level of that miRNAor set of miRNA in a normal tissue reference or a diseased tissuereference. This correlation allows for the identification ofnon-lymphoid cells in lymph nodes and enhances or provides prognosticand/or diagnostic methods. It is specifically contemplated that miRNAprofiles for patients having or suspected of having a particular diseaseor condition such as cervix, skin, colon, breast, bladder, esophagus,liver, ovary, prostate, lung, pancreas, thyroid, kidney, stomach,testicle, uterus, spleen, tongue, brain, thymus, trachea and/or smallintestine cancer can be generated by evaluating any of the sets orsubsets of the miRNAs discussed in this application. The miRNA profilethat is generated from the patient will be one that provides prognostic,diagnostic or staging information regarding the particular disease orcondition. In many embodiments, the miRNA profile is generated usingmiRNA hybridization or amplification, (e.g., array hybridization orRT-PCR). In certain aspects, a miRNA profile can be used in conjunctionwith other diagnostic tests, such as protein levels or mRNA expressionlevels.

A miRNA or probe set comprising or identifying a segment of acorresponding miRNA can include all or part of 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100,125, 150, 175, 200, 250, 300, 350, 400, 500 600, 700, 800, 900, 1,000miRNAs, including any integer or range derivable there between, of Table4, 5, 6, 10, 11, 12, 13, 14, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 25,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 39, 40, 41, 43, and/or 44.

A sample may be taken from a patient having or suspected of having adisease or pathological condition. In certain aspects, the sample canbe, but is not limited to tissue (e.g., biopsy, particularly fine needlebiopsy), blood, serum, plasma, fluids (sputum, secretions, etc.) orlymph nodes (such as sentinel, proximal to a disease site, or distalfrom a disease site). The sample can be fresh, frozen, fixed (e.g.,formalin fixed), or embedded (e.g., paraffin embedded). In a particularaspect, the sample can be a biopsy sample, or a lymph node sample.

In certain aspects of the invention a plurality of miRNAs have beenidentified that can be used to evaluate LN for the presence of a numberof different non-lymphoid tissue or cell types. This pandiagnostic orpanprognostic set of miRNAs include miRNA that are typicallydifferentially expressed between normal tissues and normal lymph node.These miRNA include one or more miRNA selected from Table 37.

It is contemplated that various subsets of the miRNA listed herein canbe used to the exclusion of other subsets of miRNA described herein.

miRNAs differentially expressed between cervix and LNneg tissue includeone or more of miRNA selected from Table 4.

miRNAs differentially expressed between LNpos and LNneg tissue incervical cancer patients include one or more miRNA selected from Table5.

miRNAs differentially expressed in both cervix versus LNneg and LNposversus LNneg include one or more miRNA selected from Table 6.

miRNAs differentially expressed between melanoma and LNneg tissueinclude one or more miRNA selected from Table 10.

miRNAs differentially expressed between LNpos and LNneg tissue frommelanoma patients include one or more miRNA selected from Table 11and/or 12.

miRNAs differentially expressed between normal colon and LNneg includeone or more miRNA selected from Table 16 and/or 17.

miRNAs differentially expressed between normal breast and LNneg includeone or more miRNA selected from Table 18.

miRNAs differentially expressed between normal bladder and LNneg includeone or more miRNA selected from Table 19.

miRNAs differentially expressed between normal esophagus and LNneginclude one or more miRNA selected from Table 20.

miRNAs differentially expressed between normal liver and LNneg includeone or more miRNA selected from Table 21.

miRNAs differentially expressed between normal ovary and LNneg includeone or more miRNA selected from Table 22.

miRNAs differentially expressed between normal prostate and LNneginclude one or more miRNA selected from Table 23.

miRNAs differentially expressed between normal lung and LNneg includeone or more miRNA selected from Table 24.

miRNAs differentially expressed between normal pancreas and LNneginclude one or more miRNA selected from Table 25.

miRNAs differentially expressed between normal thyroid and LNneg includeone or more miRNA selected from Table 26.

miRNAs differentially expressed between normal kidney and LNneg includeone or more miRNA selected from Table 27.

miRNAs differentially expressed between normal stomach and LNneg includeone or more miRNA selected from Table 28.

miRNAs differentially expressed between normal testicle and LNneginclude one or more miRNA selected from Table 29.

miRNAs differentially expressed between normal uterus and LNneg includeone or more miRNA selected from Table 30.

miRNAs differentially expressed between normal spleen and LNneg includeone or more miRNA selected from Table 31.

miRNAs differentially expressed between normal tongue and LNneg includeone or more miRNA selected from Table 32.

miRNAs differentially expressed between normal brain and LNneg includeone or more miRNA selected from Table 33.

miRNAs differentially expressed between normal thymus and LNneg includeone or more miRNA selected from Table 34.

miRNAs differentially expressed between normal trachea and LNneg includeone or more miRNA selected from Table 35.

miRNAs differentially expressed between normal small intestine and LNneginclude one or more miRNA selected from Table 36.

miRNAs differentially expressed between cancer-positive LN and/orcancer-negative LN from breast cancer patients include one or more miRNAselected from Table 39.

miRNAs differentially expressed between normal breast and LNneg includeone or more miRNA selected from Table 40.

miRNAs differentially expressed between normal breast tissue andcancer-negative axillary LN, and between LNneg and LNpos axillary LNinclude one or more miRNA selected from Table 41.

miRNAs differentially expressed between LNneg and LNpos from breastcancer patients include one or more miRNA selected from Table 43.

miRNAs differentially expressed between LNpos and LNneg from cervicalcancer patients include one or more miRNA selected from Table 44.

miRNA sequences can be found in the miRBase database (release 12.0(September 2008)). miRBase is accessible via the world wide web atmicroma.sanger.ac.uk, the content of release 12.0, as it relates to themiRNA identified in the tables, is incorporated herein by reference inits entirety.

The methods can further comprise one or more of steps including: (a)obtaining a sample from the patient, (b) isolating nucleic acids fromthe sample, (c) labeling the nucleic acids isolated from the sample, (d)hybridizing the labeled nucleic acids to one or more probes, and/orevaluating or assessing the level of expression of miRNA of interest.Once evaluation or assessment has been completed then a prognosis,diagnosis, stage of disease or other report can be generated andtransmitted to a person of interest, e.g., a physician.

Certain embodiments of the invention include determining expression ofone or more miRNA by using an amplification assay or a hybridizationassay, a variety of which are well known to one of ordinary skill in theart. In certain aspects, an amplification assay can be a quantitativeamplification assay, such as quantitative RT-PCR or the like. In stillfurther aspects, a hybridization assay can include array hybridizationassays or solution hybridization assays.

Aspects of the invention can be used to diagnose or assess a patient'scondition. For example, the methods can be used to screen for apathological condition, assess prognosis of a pathological condition,stage a pathological condition, or assess response of a pathologicalcondition to therapy.

The present invention also concerns kits containing compositions of theinvention or compositions to implement methods of the invention. In someembodiments, kits can be used to evaluate one or more miRNA molecules.In certain embodiments, a kit contains, contains at least or contains atmost 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19,20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37,38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 100, 500, 1,000 ormore miRNA probes, synthetic miRNA molecules or miRNA inhibitors, or anyvalue or range and combination derivable therein. In some embodiments,there are kits for evaluating miRNA activity in a cell.

Kits may comprise components, which may be individually packaged orplaced in a container, such as a tube, bottle, vial, syringe, or othersuitable container means.

Individual components may also be provided in a kit in concentratedamounts; in some embodiments, a component is provided individually inthe same concentration as it would be in a solution with othercomponents. Concentrations of components may be provided as 1×, 2×, 5×,10×, or 20× or more.

Kits for using miRNA probes, synthetic miRNAs, nonsynthetic miRNAs,and/or miRNA inhibitors of the invention for prognostic or diagnosticapplications are included as part of the invention. Specificallycontemplated are any such molecules corresponding to any miRNAidentified herein.

In certain aspects, negative and/or positive control synthetic miRNAsand/or miRNA inhibitors are included in some kit embodiments. Thecontrol molecules can be used to verify transfection efficiency and/orcontrol for transfection-induced changes in cells.

It is contemplated that any method or composition described herein canbe implemented with respect to any other method or composition describedherein and that different embodiments may be combined. It isspecifically contemplated that any methods and compositions discussedherein with respect to miRNA molecules or miRNA may be implemented withrespect to synthetic miRNAs to the extent the synthetic miRNA is exposedto the proper conditions to allow it to become a mature miRNA underphysiological circumstances. The claims originally filed arecontemplated to cover claims that are multiply dependent on any filedclaim or combination of filed claims.

Any embodiment of the invention involving specific miRNAs by name iscontemplated also to cover embodiments involving miRNAs whose sequencesare at least 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94,95, 96, 97, 98, 99% identical to the mature sequence of the specifiedmiRNA.

Embodiments of the invention include kits for analysis of a pathologicalsample by assessing miRNA profile for a sample comprising, in suitablecontainer means, two or more miRNA probes, wherein the miRNA probesdetect one or more of the miRNA identified herein. The kit can furthercomprise reagents for labeling miRNA in the sample. The kit may alsoinclude labeling reagents, including at least one of amine-modifiednucleotide, poly(A) polymerase, and poly(A) polymerase buffer. Labelingreagents can include an amine-reactive dye.

Other embodiments of the invention are discussed throughout thisapplication. Any embodiment discussed with respect to one aspect of theinvention applies to other aspects of the invention as well and viceversa. The embodiments in the Example section are understood to beembodiments of the invention that are applicable to all aspects of theinvention.

The terms “inhibiting,” “reducing,” or “prevention,” or any variation ofthese terms, when used in the claims and/or the specification includesany measurable decrease or complete inhibition to achieve a desiredresult.

The use of the word “a” or “an” when used in conjunction with the term“comprising” in the claims and/or the specification may mean “one,” butit is also consistent with the meaning of “one or more,” “at least one,”and “one or more than one.”

It is contemplated that any embodiment discussed herein can beimplemented with respect to any method or composition of the invention,and vice versa. Furthermore, compositions and kits of the invention canbe used to achieve methods of the invention.

Throughout this application, the term “about” is used to indicate that avalue includes the standard deviation of error for the device or methodbeing employed to determine the value.

The use of the term “or” in the claims is used to mean “and/or” unlessexplicitly indicated to refer to alternatives only or the alternativesare mutually exclusive, although the disclosure supports a definitionthat refers to only alternatives and “and/or.”

As used in this specification and claim(s), the words “comprising” (andany form of comprising, such as “comprise” and “comprises”), “having”(and any form of having, such as “have” and “has”), “including” (and anyform of including, such as “includes” and “include”) or “containing”(and any form of containing, such as “contains” and “contain”) areinclusive or open-ended and do not exclude additional, unrecitedelements or method steps.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and the specificexamples, while indicating specific embodiments of the invention, aregiven by way of illustration only, since various changes andmodifications within the spirit and scope of the invention will becomeapparent to those skilled in the art from this detailed description.

DESCRIPTION OF THE DRAWINGS

The following drawings form part of the present specification and areincluded to further demonstrate certain aspects of the presentinvention. The invention may be better understood by reference to one ormore of these drawings in combination with the detailed description ofspecific embodiments presented herein.

FIGS. 1A-1B Differences in expression levels of eleven miRNAs, betweenLNpos1-4 and LNneg1-4 samples from cervical cancer patients, measured byqRT-PCR and microarray quantification. The graphs show the individualnormalized expression levels determined by array or qRT-PCR andassociated p-values (pair-wise t-test).

FIGS. 2A-2D qRT-PCR quantification of eleven microRNAs in twoindependent sets of cancer-positive (LNpos) and cancer-negative (LNneg)lymph nodes obtained from cervical cancer patients. (FIG. 2A) SamplesLNpos1-4 and LNneg1-4. FIG. 2B. Samples LNpos5-12 and LNneg5-8. (FIG.2C) Combined qRT-PCR data for samples LNpos1-12 and LNneg1-8. (FIG. 2D)Summary table of the qRT-PCR data with fold changes and p-values forLNpos1-4/LNneg1-4, LNpos5-12/LNneg5-8, and LNpos1-12 and LNneg1-8.

FIG. 3 Differences in expression levels of various miRNA combinations,between LNpos1-4 and LNneg1-4 samples from cervical cancer patients,measured by qRT-PCR and microarray quantification. For qRT-PCR assays,the difference in Ct between the combined normalized Ct values in eachsample group is indicated on each graph. For array quantificationassays, the difference in the combined ΔH values for each sample groupis indicated on each graph.

FIG. 4 Sensitivity of detection of synthetic miR-205 and miR-203 in abackground of total RNA purified from a cervical cancer-negativeinguinal lymph node (LNneg). The number of copies of each individualsynthetic miRNA added to a background of LNneg total RNA are indicatedon the X axis. Mean Ct values indicated on the Y axis represent the meanof duplicated qRT-PCR reactions. Error bars in the figures represent thevalues for each of the two reactions. Both miR-16 and miR-24 were usedas internal controls.

FIGS. 5A-5B (FIG. 5A) Sensitivity of detection of five miRNAs (miR-205,-429, -200a, -200c, and -141) determined by qRT-PCR, following dilutionof total RNA from a cervical tumor (TUM) into total RNA from acancer-negative inguinal lymph node (LNneg). Arrows indicate limits ofdetection. Mean Ct, mean of duplicate qRT-PCR reactions. NTC, notemplate control (i.e., no cervical tumor RNA added). (FIG. 5B)Sensitivity of detection of five miRNAs (miR-205, -429, -200a, -200c,and -141) determined by qRT-PCR, following dilution of total RNA from acancer-positive lymph node (LNpos) into total RNA from a cancer-negativelymph node (LNneg). Arrows indicate limits of detection. Mean Ct, meanof duplicate qRT-PCR reactions. NTC, no template control (i.e., no LNposRNA added).

FIG. 6 Principal component analysis (PCA) of miRNAs differentiallyexpressed in melanoma, cancer-positive lymph node group A(LNposA-LNpos1A), cancer-positive lymph node group B (LNposB-LNpos2B,LNpos3B), and cancer-negative lymph node (LNneg1-7) samples frommelanoma patients, as determined by ANOVA.

FIGS. 7A-7C Comparison of array and qRT-PCR data for selected miRNAsdifferentially expressed between melanoma (MEL1, MEL3) andcancer-negative lymph nodes (LNneg1-7). (FIG. 7A) Normalized array datafor indicated miRNAs. (FIG. 7B) qRT-PCR data obtained with primer setsfor the indicated miRNAs, normalized to miR-16 expression level for eachsamples (miRNACt-miR-16Ct). (FIG. 7C) Associated p-values and foldchange for each miRNA as determined by microarray expression analysis orqRT-PCR.

FIGS. 8A-8C Comparison of array and qRT-PCR data for selected miRNAsdifferentially expressed between the cancer-positive group A (LNpos1A)lymph node and cancer-negative lymph nodes (LNneg) from melanomapatients. (FIG. 8A) Normalized array data for indicated miRNAs. (FIG.8B) qRT-PCR data obtained with primer sets for the indicated miRNAs,normalized to miR-16 expression level for each samples(miRNACt-miR-16Ct). (FIG. 8C) Associated p-values and fold change foreach miRNA as determined by microarray expression analysis or qRT-PCR.

FIGS. 9A-9C Comparison of array and qRT-PCR data for selected miRNAsdifferentially expressed between the cancer-positive group B (LNpos2B,3B) lymph nodes and cancer-negative lymph nodes (LNneg1-7) from melanomapatients. (FIG. 9A) Normalized array data for indicated miRNAs. (FIG.9B) qRT-PCR data obtained with primer sets for the indicated miRNAs,normalized to miR-16 expression level for each sample(miRNACt-miR-16Ct). (FIG. 9C) Associated p-values and fold change foreach miRNA as determined by microarray expression analysis or qRT-PCR.

FIG. 10 Differences in expression levels of ten miRNAs, betweencancer-positive lymph nodes from colon cancer patients (LNpos) andcancer-negative lymph nodes (LNneg), measured by qRT-PCR The graph showsthe individual normalized expression levels (dCt=miRNACt−miR-16Ct).Differences in the normalized mean expression values between the twosample sets were transformed to a general fold change (FC) byexponentiation base 2, as indicated below the graph with the associatedp-values (pair-wise t-test).

FIG. 11 Differences in expression levels of various miRNA combinations,between cancer-positive (LNpos) lymph nodes from colon cancer patientsand cancer-negative lymph nodes (LNneg), measured by qRT-PCR. Thedifference in Ct between the combined mean normalized Ct values in eachsample group and the associated p-value are indicated on each graph.miR-16 was used as the normalizer.

FIGS. 12A-12B Six miRNAs (miR-429, -141, -203, -200a, -200b, and -200c)can distinguish positive and negative lymph nodes from patients witheither cervical colon or breast cancer. The graphs show (FIG. 12A) theindividual normalized expression levels determined by qRT-PCR for theindicated miRNAs for each positive and negative lymph node analyzed ineach cancer group. (FIG. 12B) Differences in expression levels of twocombination sets of miRNAs between positive and negative lymph nodesfrom patients with cervical cancer, colon cancer and breast cancer,measured by qRT-PCR quantification. The miRNAs used in combinations areindicated above the graphs. The difference in Ct between the combinednormalized Ct values in each sample group is indicated on each graph.

FIGS. 13A-13B Comparison of array and qRT-PCR data for selected miRNAsdifferentially expressed between normal breast (NBr) tissue andcancer-positive (LNpos) and cancer-negative (LNneg) axillary lymph nodesfrom ductal breast cancer patients. (FIG. 13A) Normalized array data forindicated miRNAs. (FIG. 13B) qRT-PCR data obtained using specific primersets for each indicated miRNA, normalized to miR-16 expression level(miRNACt-miR-16Ct). Associated p-values (pair-wise-t-test) indicated ontop of each graph represents the p-values for NBr and LNneg comparison(p) and the p-values for the comparison of the paired LNpos and LNnegsamples (p*).

FIGS. 14A-14B Differences in mean expression levels of ten miRNAs,between cancer-positive (LNpos) and cancer-negative (LNneg) lymph nodesfrom breast cancer patients. Data for two independent sets of lymphnodes, represented by FIG. 14A (LNpos2-4 and LNneg1-4) and FIG. 14B(LNpos5-14 and LNneg5-9). Graphs show the mean expression levels of eachmiRNA after normalization to both miR-16 and miR-638(dCt=CtmiRNA−1/2(CtmiR16+CtmiR-638)). Ct values indicated on the y-axisrepresent the mean of duplicate qRT-PCR reactions. Error bars representthe values for each of the two reactions. Differences in the normalizedmean expression values between the LNpos and LNneg of each sample setwere transformed to a general fold change (FC) by exponentiation base 2as indicated below each graph. Associated p-values (pair-wise t-test) ofthe comparison between the paired LNpos and LNneg samples (FIG. 14A-p*)and between the independent and unmatched set of LNpos and LNneg samples(FIG. 14B-p) are indicated below each graph.

FIG. 15 Combined qRT-PCR data of ten miRNAs for two independent sets ofcancer-positive (LNpos2-14) and cancer-negative (LNneg1-9) lymph nodesfrom breast cancer patients. For each qRT-PCR assay, the difference inCt between the combined normalized Ct values in each sample group wastransformed to a general average fold change (Av.FC) by exponentiationbase 2, as indicated on each graph, with associated p-values.

FIG. 16 Differences in expression levels of various miRNA combinations,between LNpos2-14 and LNneg1-9) samples from breast cancer patients, asmeasured by qRT-PCR. The difference in Ct between the combinednormalized Ct values in each sample group is indicated on the threefirst graphs. For the two last combinations, miR-429-miR-150 andmiR-200a-miR-150, the difference in Ct between the two sample groups isexpressed by the subtraction of the raw Ct values of each miRNA.

FIGS. 17A-17B qRT-PCR quantification of 14 selected miRNAs in FFPEmelanoma samples (MEL4-6) and FFPE cancer-positive lymph nodes(LNpos5-8) from melanoma patients compared to cancer-negative lymphnodes (LNneg1-7). (FIG. 17A) Comparison between MEL4-6 and LNneg1-7, asindicated on the graph. (FIG. 17B) Comparison between LNpos5-8 andLNneg1-7 samples, as indicated on the graph. Values represent the meanCt of duplicated qRT-PCR reactions. miR-16 was used as a normalizer.Error bars in the figures represent the values for each of the twoqRT-PCR reactions. For each miRNA, the associated fold change andp-value (student t-test) are indicated below each graph.

FIGS. 18A-18B Differences in expression levels of fourteen miRNAsbetween cancer-positive (LNpos) and cancer-negative (LNneg) lymph nodesfrom melanoma patients. (FIG. 18A) The graph shows the qRT-PCR dataobtained with primer sets specific for the indicated miRNAs, normalizedto miR-16 expression level (miRNACt-miR-16Ct) in LNneg1-7, LNposA(LNpos1A), LNposB (LNpos2-7), and LNpos8, as indicated on the graph.(FIG. 18B) Summary table of the data with fold-changes and associatedp-values for LNposA vs LNneg and LNposB vs LNneg.

FIGS. 19A-19B Differences in expression levels of various miRNAcombinations, between melanomas (MEL), cancer-negative (LNneg) lymphnodes, and cancer-positive (LNpos) lymph nodes. The miRNAs used incombinations are indicated above the graphs. (FIG. 19A) The graphs showthe combined mean Ct values in each of LNneg, LNpos, and MEL samples,normalized to miR-16. (FIG. 19B) The table displays the fold changesobserved between the mean Ct values of each sample group.

DETAILED DESCRIPTION OF THE INVENTION

Embodiments of the present invention are directed to compositions andmethods relating to preparation and characterization of miRNAs, as wellas to the use of miRNAs for therapeutic, prognostic, diagnostic, andstaging applications, particularly those methods and compositionsrelated to assessing and/or identifying a non-lymphoid cell in a lymphnode tissue.

I. miRNA Molecules

The term “miRNA” is used according to its ordinary and plain meaning andrefers to a microRNA molecule found in eukaryotes that is involved inRNA-based gene regulation. See, e.g., Carrington et al., 2003, which ishereby incorporated by reference. The term will be used to refer to thesingle-stranded RNA molecule processed from a precursor. IndividualmiRNAs have been identified and sequenced in different organisms, andthey have been given names. Names of miRNAs related to the presentinvention are provided herein. The sequences related to these miRNA areknow and available through public resources such as scientficpublications, miRBase 12.0 and other nucleic acid databases. The methodsand compositions should not be limited to miRNAs identified in theapplication, as they are provided as examples, not necessarily aslimitations of the invention.

MicroRNA molecules (“miRNAs”) are generally 21 to 22 nucleotides inlength, though lengths of 16 and up to 35 nucleotides have beenreported. The miRNAs are each processed from a longer precursor RNAmolecule (“precursor miRNA”). Precursor miRNAs are transcribed fromnon-protein-encoding genes. The precursor miRNAs have two regions ofcomplementarity that enables them to form a stem-loop- or fold-back-likestructure, which is cleaved in animals by a ribonuclease III-likenuclease enzyme called Dicer. The processed miRNA is typically a portionof the stem.

The processed miRNA (also referred to as “mature miRNA”) become part ofa large complex to down-regulate a particular target gene. Examples ofanimal miRNAs include those that imperfectly basepair with the target,which halts translation (Olsen et al., 1999; Seggerson et al., 2002).siRNA molecules also are processed by Dicer, but from a long,double-stranded RNA molecule. siRNAs are not naturally found in animalcells, but they can direct the sequence-specific cleavage of an mRNAtarget through a RNA-induced silencing complex (RISC) (Denli et al,2003).

A. Nucleic Acids

The present invention concerns miRNAs that can be labeled, used in arrayanalysis, or employed in diagnostic, therapeutic, prognostic, and/orstaging applications, particularly those related to pathological and/orcancerous conditions. The RNA may have been endogenously produced by acell, or been synthesized or produced chemically or recombinantly. Theymay be isolated and/or purified. The term “miRNA,” unless otherwiseindicated, refers to the processed RNA, after it has been cleaved fromits precursor. Mature and precursor sequences of miRNA can typically belocated in Sanger miRBase™ accessible on the internet atmicrorna.sanger.ac.uk/ or in various scientific publications. The nameof the miRNA is often abbreviated and referred to without a hsa-, mmu-,or rno-prefix and will be understood as such, depending on the context.Unless otherwise indicated, miRNAs referred to in the application arehuman sequences identified as miR-X or let-X, where X is a number and/orletter.

It is understood that a “synthetic nucleic acid” of the invention meansthat the nucleic acid does not have a chemical structure or sequence ofa naturally occurring nucleic acid. Consequently, it will be understoodthat the term “synthetic miRNA” refers to a “synthetic nucleic acid”that functions in a cell or under physiological conditions as anaturally occurring miRNA.

While many of the embodiments of the invention involve synthetic miRNAsor synthetic nucleic acids, in some embodiments of the invention, thenucleic acid molecule(s) need not be “synthetic.” The term miRNA willgenerically refer to both non-synthetic (e.g., endogenous) miRNA andsynthetic miRNA. In certain embodiments, a non-synthetic miRNA employedin methods and compositions of the invention may have the entiresequence and structure of a naturally occurring miRNA precursor or themature miRNA. For example, non-synthetic miRNAs used in methods andcompositions of the invention may not have one or more modifiednucleotides or nucleotide analogs. In these embodiments, thenon-synthetic miRNA may or may not be recombinantly produced. Anyembodiments discussed with respect to the use of synthetic miRNAs can beapplied with respect to non-synthetic miRNAs, and vice versa.

It will be understood that the term “naturally occurring” refers tosomething found in an organism without any intervention by a person; itcould refer to a naturally-occurring wildtype or mutant molecule. Insome embodiments a synthetic miRNA molecule does not have the sequenceof a naturally occurring miRNA molecule. In other embodiments, asynthetic miRNA molecule may have the sequence of a naturally occurringmiRNA molecule, but the chemical structure of the molecule, particularlyin the part unrelated specifically to the precise sequence (non-sequencechemical structure) differs from chemical structure of the naturallyoccurring miRNA molecule with that sequence. In some cases, thesynthetic miRNA has both a sequence and non-sequence chemical structurethat are not found in a naturally-occurring miRNA. Moreover, thesequence of the synthetic molecules will identify which miRNA iseffectively being provided or inhibited; the endogenous miRNA will bereferred to as the “corresponding miRNA.” Corresponding miRNA sequencesthat can be used in the context of the invention include, but are notlimited to, all or a portion of those sequences referred to herein, aswell as any other miRNA sequence, miRNA precursor sequence, or anysequence complementary thereof. In some embodiments, the sequence is oris derived from or contains all or part of a sequence identified hereinto target or identify or measure a particular miRNA (or set of miRNAs).

In certain experiments, a miRNA probe designated by a suffix “5P” or“3P” can be used. “5P” indicates that the mature miRNA derives from the5′ end of the precursor and a corresponding “3P” indicates that itderives from the 3′ end of the precursor, as described on the miRBase™website. Moreover, in some embodiments, a miRNA probe is used that doesnot correspond to a known human miRNA. It is contemplated that thesenon-human miRNA probes may be used in embodiments of the invention orthat there may exist a human miRNA that is homologous to the non-humanmiRNA. While the invention is not limited to human miRNA, in certainembodiments, miRNA from human cells or a human biological sample isevaluated. In other embodiments, any mammalian cell, biological sample,or preparation thereof may be employed.

In some embodiments of the invention, methods and compositions involvingmiRNA may concern miRNA and/or other nucleic acids. Nucleic acids maybe, be at least, or be at most 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32,33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68,69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103,104, 105, 106, 107, 108, 109, 110, 120, 130, 140, 150, 160, 170, 180,190, 200, 210, 220, 230, 240, 250, 260, 270, 280, 290, 300, 310, 320,330, 340, 350, 360, 370, 380, 390, 400, 410, 420, 430, 440, 450, 460,470, 480, 490, 500, 510, 520, 530, 540, 550, 560, 570, 580, 590, 600,610, 620, 630, 640, 650, 660, 670, 680, 690, 700, 710, 720, 730, 740,750, 760, 770, 780, 790, 800, 810, 820, 830, 840, 850, 860, 870, 880,890, 900, 910, 920, 930, 940, 950, 960, 970, 980, 990, 1000, or 10,000nucleotides or more in length, including any range derivable therein, inlength. Such lengths cover the lengths of processed miRNA, miRNA probes,precursor miRNA, miRNA containing vectors, control nucleic acids, andother probes and primers. In many embodiments, miRNA are 19-24nucleotides in length, while miRNA probes are 19-35 nucleotides inlength, depending on the length of the processed miRNA and any flankingregions added. miRNA precursors are generally between 62 and 110nucleotides in humans.

Nucleic acids of the invention may have regions of identity orcomplementarity to another nucleic acid. It is contemplated that theregion of complementarity or identity can be at least 5 contiguousresidues, though it is specifically contemplated that the region is, isat least, or is at most 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18,19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36,37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54,55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72,73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90,91, 92, 93, 94, 95, 96, 97, 98, 99, 100, or 110 contiguous nucleotidesincluding ranges there between. It is further understood that the lengthof complementarity within a precursor miRNA or between a miRNA probe anda miRNA or a miRNA gene are such lengths. Moreover, the complementaritymay be expressed as a percentage, meaning that the complementarity oridentity between a probe and its target is 90% or greater over thelength of the probe. In some embodiments, complementarity is or is atleast 90%, 95% or 100%. In particular, such lengths may be applied toany nucleic acid comprising a nucleic acid sequence identified ordisclosed herein. The commonly used name of the miRNA is given (with itsidentifying source in the prefix, for example, “hsa” for humansequences) and the processed miRNA sequence. Unless otherwise indicated,a miRNA without a prefix will be understood to refer to a human miRNA. AmiRNA designated, for example, as miR-1-2 in the application will beunderstood to refer to hsa-miR-1-2. Moreover, a lowercase letter in onereference may or may not be lowercase in another; for example,hsa-mir-130b can also be referred to as miR-130B. Other prefix can befound using the miRBase website or similar databases. The term “miRNAprobe” refers to a nucleic acid probe that can identify a particularmiRNA or structurally related miRNAs.

The term “recombinant” may be used and this generally refers to amolecule that has been manipulated in vitro or that is a replicated orexpressed product of such a molecule.

The term “nucleic acid” is well known in the art. A “nucleic acid” asused herein will generally refer to a molecule (one or more strands) ofDNA, RNA or a derivative or analog thereof, comprising a nucleobase. Anucleobase includes, for example, a naturally occurring purine orpyrimidine base found in DNA (e.g., an adenine “A,” a guanine “G,” athymine “T” or a cytosine “C”) or RNA (e.g., an A, a G, an uracil “U” ora C). The term “nucleic acid” encompasses the terms “oligonucleotide”and “polynucleotide,” each as a subgenus of the term “nucleic acid.”

The term “miRNA” generally refers to a single-stranded molecule, but inspecific embodiments, molecules implemented in the invention will alsoencompass a region or an additional strand that is partially (between 10and 50% complementary across length of strand), substantially (greaterthan 50% but less than 100% complementary across length of strand) orfully complementary to another region of the same single-strandedmolecule or to another nucleic acid. Thus, nucleic acids may encompass amolecule that comprises one or more complementary or self-complementarystrand(s) or “complement(s)” of a particular sequence comprising amolecule. For example, precursor miRNA may have a self-complementaryregion, which is up to 100% complementary. miRNA probes or nucleic acidsof the invention can include, can be or can be at least 60, 65, 70, 75,80, 85, 90, 95, 96, 97, 98, 99 or 100% complementary to their target.

As used herein, “hybridization”, “hybridizes” or “capable ofhybridizing” is understood to mean the forming of a double or triplestranded molecule or a molecule with partial double or triple strandednature. The term “anneal” as used herein is synonymous with “hybridize.”The term “hybridization”, “hybridize(s)” or “capable of hybridizing”encompasses the terms “stringent condition(s)” or “high stringency” andthe terms “low stringency” or “low stringency condition(s).”

As used herein “stringent condition(s)” or “high stringency” are thoseconditions that allow hybridization between or within one or morenucleic acid strand(s) containing complementary sequence(s), butpreclude hybridization of random sequences. Stringent conditionstolerate little, if any, mismatch between a nucleic acid and a targetstrand. Such conditions are well known to those of ordinary skill in theart, and are preferred for applications requiring high selectivity.Non-limiting applications include isolating a nucleic acid, such as agene or a nucleic acid segment thereof, or detecting at least onespecific mRNA transcript or a nucleic acid segment thereof, and thelike.

Stringent conditions may comprise low salt and/or high temperatureconditions, such as provided by about 0.02 M to about 0.5 M NaCl attemperatures of about 42° C. to about 70° C. It is understood that thetemperature and ionic strength of a desired stringency are determined inpart by the length of the particular nucleic acid(s), the length andnucleobase content of the target sequence(s), the charge composition ofthe nucleic acid(s), and the presence or concentration of formamide,tetramethylammonium chloride or other solvent(s) in a hybridizationmixture.

It is also understood that these ranges, compositions and conditions forhybridization are mentioned by way of non-limiting examples only, andthat the desired stringency for a particular hybridization reaction isoften determined empirically by comparison to one or more positive ornegative controls. Depending on the application envisioned it ispreferred to employ varying conditions of hybridization to achievevarying degrees of selectivity of a nucleic acid towards a targetsequence. In a non-limiting example, identification or isolation of arelated target nucleic acid that does not hybridize to a nucleic acidunder stringent conditions may be achieved by hybridization at lowtemperature and/or high ionic strength. Such conditions are termed “lowstringency” or “low stringency conditions,” and non-limiting examples oflow stringency include hybridization performed at about 0.15 M to about0.9 M NaCl at a temperature range of about 20° C. to about 50° C. Ofcourse, it is within the skill of one in the art to further modify thelow or high stringency conditions to suite a particular application.

In some embodiments, there is a synthetic miRNA having a length ofbetween 17 and 130 residues. The present invention concerns syntheticmiRNA molecules that are, are at least, or are at most 8, 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30,31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48,49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84,85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115,116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129,130, 140, 145, 150, 160, 170, 180, 190, 200 or more residues in length,including any integer or any range derivable therein.

In certain embodiments, synthetic miRNA have (a) a “miRNA region” whosesequence from 5′ to 3′ is identical to all or a segment of a maturemiRNA sequence, and (b) a “complementary region” whose sequence from 5′to 3′ is between 60% and 100% complementary to the miRNA sequence. Incertain embodiments, these synthetic miRNA are also isolated, i.e.,. Theterm “miRNA region” refers to a region on the synthetic miRNA that is atleast 75, 80, 85, 90, 95, or 100% identical, including all integersthere between, to the entire sequence of a mature, naturally occurringmiRNA sequence. In certain embodiments, the miRNA region is or is atleast 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 99.1, 99.2, 99.3, 99.4,99.5, 99.6, 99.7, 99.8, 99.9 or 100% identical to the sequence of anaturally-occurring miRNA.

The term “complementary region” refers to a region of a synthetic miRNAthat is or is at least 60% complementary to the mature, naturallyoccurring miRNA sequence that the miRNA region is identical to. Thecomplementary region is or is at least 60, 61, 62, 63, 64, 65, 66, 67,68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85,86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 99.1, 99.2,99.3, 99.4, 99.5, 99.6, 99.7, 99.8, 99.9 or 100% complementary, or anyrange derivable therein. With single polynucleotide sequences, there isa hairpin loop structure as a result of chemical bonding between themiRNA region and the complementary region. In other embodiments, thecomplementary region is on a different nucleic acid molecule than themiRNA region, in which case the complementary region is on thecomplementary strand and the miRNA region is on the active strand.

In other embodiments of the invention, there are synthetic nucleic acidsthat are miRNA inhibitors. A miRNA inhibitor is between about 17 to 25nucleotides in length and comprises a 5′ to 3′ sequence that is at least90% complementary to the 5′ to 3′ sequence of a mature miRNA. In certainembodiments, a miRNA inhibitor molecule is 17, 18, 19, 20, 21, 22, 23,24, or 25 nucleotides in length, or any range derivable therein.Moreover, a miRNA inhibitor has a sequence (from 5′ to 3′) that is or isat least 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 99.1, 99.2, 99.3, 99.4,99.5, 99.6, 99.7, 99.8, 99.9 or 100% complementary, or any rangederivable therein, to the 5′ to 3′ sequence of a mature miRNA,particularly a mature, naturally occurring miRNA. One of skill in theart could use a portion of the probe sequence that is complementary tothe sequence of a mature miRNA as the sequence for a miRNA inhibitor.Moreover, that portion of the probe sequence can be altered so that itis still 90% complementary to the sequence of a mature miRNA.

In some embodiments, of the invention, a synthetic miRNA contains one ormore design elements. These design elements include, but are not limitedto: (i) a replacement group for the phosphate or hydroxyl of thenucleotide at the 5′ terminus of the complementary region; (ii) one ormore sugar modifications in the first or last 1 to 6 residues of thecomplementary region; or, (iii) noncomplementarity between one or morenucleotides in the last 1 to 5 residues at the 3′ end of thecomplementary region and the corresponding nucleotides of the miRNAregion.

In certain embodiments, a synthetic miRNA has a nucleotide at its 5′ endof the complementary region in which the phosphate and/or hydroxyl grouphas been replaced with another chemical group (referred to as the“replacement design”). In some cases, the phosphate group is replaced,while in others, the hydroxyl group has been replaced. In particularembodiments, the replacement group is biotin, an amine group, a loweralkylamine group, an aminohexyl phosphate group, an acetyl group, 2′O-Me(2′oxygen-methyl), DMTO (4,4′-dimethoxytrityl with oxygen), fluoroscein,a thiol, or acridine, though other replacement groups are well known tothose of skill in the art and can be used as well. This design elementcan also be used with a miRNA inhibitor.

Additional embodiments concern a synthetic miRNA having one or moresugar modifications in the first or last 1 to 6 residues of thecomplementary region (referred to as the “sugar replacement design”). Incertain cases, there is one or more sugar modifications in the first 1,2, 3, 4, 5, 6 or more residues of the complementary region, or any rangederivable therein. In additional cases, there is one or more sugarmodifications in the last 1, 2, 3, 4, 5, 6 or more residues of thecomplementary region, or any range derivable therein, have a sugarmodification. It will be understood that the terms “first” and “last”are with respect to the order of residues from the 5′ end to the 3′ endof the region. In particular embodiments, the sugar modification is a2′O-Me modification, a 2° F. modification, a 2′H modification, a 2′aminomodification, a 4′ribose modification, or a phosphorothioatemodification on the carboxy group linked to the carbon at position 6. Infurther embodiments, there is one or more sugar modifications in thefirst or last 2 to 4 residues of the complementary region or the firstor last 4 to 6 residues of the complementary region. This design elementcan also be used with a miRNA inhibitor. Thus, a miRNA inhibitor canhave this design element and/or a replacement group on the nucleotide atthe 5′ terminus, as discussed above.

In other embodiments of the invention, there is a synthetic miRNA inwhich one or more nucleotides in the last 1 to 5 residues at the 3′ endof the complementary region are not complementary to the correspondingnucleotides of the miRNA region (“noncomplementarity”) (referred to asthe “noncomplementarity design”). The noncomplementarity may be in thelast 1, 2, 3, 4, and/or 5 residues of the complementary miRNA. Incertain embodiments, there is noncomplementarity with at least 2nucleotides in the complementary region.

It is contemplated that synthetic miRNA of the invention have one ormore of the replacement, sugar modification, or noncomplementaritydesigns. In certain cases, synthetic RNA molecules have two of them,while in others these molecules have all three designs in place.

The miRNA region and the complementary region may be on the same orseparate polynucleotides. In cases in which they are contained on or inthe same polynucleotide, the miRNA molecule will be considered a singlepolynucleotide. In embodiments in which the different regions are onseparate polynucleotides, the synthetic miRNA will be considered to becomprised of two polynucleotides.

When the RNA molecule is a single polynucleotide, there is a linkerregion between the miRNA region and the complementary region. In someembodiments, the single polynucleotide is capable of forming a hairpinloop structure as a result of bonding between the miRNA region and thecomplementary region. The linker constitutes the hairpin loop. It iscontemplated that in some embodiments, the linker region is, is atleast, or is at most 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16,17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34,35, 36, 37, 38, 39, or 40 residues in length, or any range derivabletherein. In certain embodiments, the linker is between 3 and 30 residues(inclusive) in length.

In addition to having a miRNA region and a complementary region, theremay be flanking sequences as well at either the 5′ or 3′ end of theregion. In some embodiments, there is or is at least 1, 2, 3, 4, 5, 6,7, 8, 9, 10 nucleotides or more, or any range derivable therein,flanking one or both sides of these regions.

Methods of the invention include reducing or eliminating activity of oneor more miRNAs in a cell comprising introducing into a cell a miRNAinhibitor; or supplying or enhancing the activity of one or more miRNAsin a cell. The present invention also concerns inducing certain cellularcharacteristics by providing to a cell a particular nucleic acid, suchas a specific synthetic miRNA molecule or a synthetic miRNA inhibitormolecule. However, in methods of the invention, the miRNA molecule ormiRNA inhibitor need not be synthetic. They may have a sequence that isidentical to a naturally occurring miRNA or they may not have any designmodifications. In certain embodiments, the miRNA molecule and/or a miRNAinhibitor are synthetic, as discussed above.

The particular nucleic acid molecule provided to the cell is understoodto correspond to a particular miRNA in the cell, and thus, the miRNA inthe cell is referred to as the “corresponding miRNA.” In situations inwhich a named miRNA molecule is introduced into a cell, thecorresponding miRNA will be understood to be the induced miRNA. It iscontemplated, however, that the miRNA molecule introduced into a cell isnot a mature miRNA but is capable of becoming a mature miRNA under theappropriate physiological conditions. In cases in which a particularcorresponding miRNA is being inhibited by a miRNA inhibitor, theparticular miRNA will be referred to as the targeted miRNA. It iscontemplated that multiple corresponding miRNAs may be involved. Inparticular embodiments, more than one miRNA molecule is introduced intoa cell. Moreover, in other embodiments, more than one miRNA inhibitor isintroduced into a cell. Furthermore, a combination of miRNA molecule(s)and miRNA inhibitor(s) may be introduced into a cell.

Methods include identifying a cell or patient in need of inducing thosecellular characteristics. Also, it will be understood that an amount ofa synthetic nucleic acid that is provided to a cell or organism is an“effective amount,” which refers to an amount needed to achieve adesired goal, such as inducing a particular cellular characteristic(s).

In certain embodiments of the methods include providing or introducingto a cell a nucleic acid molecule corresponding to a mature miRNA in thecell in an amount effective to achieve a desired physiological result.

Moreover, methods can involve providing synthetic or nonsynthetic miRNAmolecules. It is contemplated that in these embodiments, methods may ormay not be limited to providing only one or more synthetic miRNAmolecules or only on or more nonsynthetic miRNA molecules. Thus, incertain embodiments, methods may involve providing both synthetic andnonsynthetic miRNA molecules. In this situation, a cell or cells aremost likely provided a synthetic miRNA molecule corresponding to aparticular miRNA and a nonsynthetic miRNA molecule corresponding to adifferent miRNA. Furthermore, any method articulated a list of miRNAsusing Markush group language may be articulated without the Markushgroup language and a disjunctive article (i.e., or) instead, and viceversa.

In some embodiments, there is a method for reducing or inhibiting cellproliferation in a cell comprising introducing into or providing to thecell an effective amount of (i) a miRNA inhibitor molecule or (ii) asynthetic or nonsynthetic miRNA molecule that corresponds to a miRNAsequence. In certain embodiments the methods involves introducing intothe cell an effective amount of (i) a miRNA inhibitor molecule having a5′ to 3′ sequence that is at least 90% complementary to the 5′ to 3′sequence of one or more mature miRNA described herein.

Certain embodiments of the invention include methods of treating apancreatic condition. In one aspect, the method comprises contacting apancreatic cell with one or more nucleic acid, synthetic miRNA, or miRNAcomprising at least one nucleic acid segment having all or a portion ofa miRNA sequence. The segment may be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 30 or more nucleotides ornucleotide analog, including all integers there between. An aspect ofthe invention includes the modulation of a miRNA or a mRNA within atarget cell.

Typically, an endogenous gene, miRNA or mRNA is modulated in the cell.In particular embodiments, the nucleic acid sequence comprises at leastone segment that is at least 70, 75, 80, 85, 90, 95, or 100% identicalin nucleic acid sequence to one or more miRNA sequence described herein.Modulation of the expression or processing of an endogenous gene, miRNA,or mRNA can be through modulation of the processing of a mRNA, suchprocessing including transcription, transportation and/or translationwith in a cell. Modulation may also be effected by the inhibition orenhancement of miRNA activity with a cell, tissue, or organ. Suchprocessing may effect the expression of an encoded product or thestability of the mRNA. In still other embodiments, a nucleic acidsequence can comprise a modified nucleic acid sequence.

Methods of the invention can further comprise administering a secondtherapy, such as chemotherapy, radiotherapy, surgery, or immunotherapy.The nucleic acid can be transcribed from a nucleic acid vector, such asa plasmid vector or a viral vector.

Method of treating a cancerous or hyperproliferative condition includecontacting or administering to a target cell with one or more nucleicacid comprise a miRNA sequence, wherein expression of an endogenousmiRNA is modulated in cancerous cell; where the miRNA sequence is atleast 70, 75, 80, 85% or more identical to one or more of the identifiedmiRNAs.

The methods may further comprise administering a second therapy. Thesecond therapy can be, but is not limited to chemotherapy, radiotherapy,surgery, or immunotherapy.

In still further aspects, one or more miRNA are transcribed from anucleic acid vector, such as a plasmid or viral vector.

Synthetic nucleic acids can be administered to the subject or patientusing modes of administration that are well known to those of skill inthe art, particularly for therapeutic applications. It is particularlycontemplated that a patient is human or any other mammal or animalhaving miRNA.

It will be understood in methods of the invention that a cell or otherbiological matter such as an organism (including patients) can beprovided a miRNA or miRNA molecule corresponding to a particular miRNAby administering to the cell or organism a nucleic acid molecule thatfunctions as the corresponding miRNA once inside the cell. The form ofthe molecule provided to the cell may not be the form that acts as amiRNA once inside the cell. Thus, it is contemplated that in someembodiments, biological matter is provided a synthetic miRNA or anonsynthetic miRNA, such as one that becomes processed into a mature andactive miRNA once it has access to the cell's miRNA processingmachinery. In certain embodiments, it is specifically contemplated thatthe miRNA molecule provided to the biological matter is not a maturemiRNA molecule but a nucleic acid molecule that can be processed intothe mature miRNA once it is accessible to miRNA processing machinery.The term “nonsynthetic” in the context of miRNA means that the miRNA isnot “synthetic,” as defined herein. Furthermore, it is contemplated thatin embodiments of the invention that concern the use of syntheticmiRNAs, the use of corresponding nonsynthetic miRNAs is also consideredan aspect of the invention, and vice versa.

In other embodiments, the methods involve reducing cell viabilitycomprising introducing into or providing to the cell an effective amountof (i) a miRNA inhibitor molecule or (ii) a synthetic or nonsyntheticmiRNA molecule that corresponds to a miRNA sequence. Methods forinducing apoptosis have a number of therapeutic applications including,but not limited to, the treatment of cancer.

The present invention also concerns using miRNA compositions to treatdiseases or conditions or to prepare therapeutics for the treatment ofdiseases or conditions. It is contemplated that 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or 40 more miRNA (orany range derivable therein) may be used for these embodiments. Incertain embodiments, methods involve one or more miRNA inhibitors and/ora miRNA molecules corresponding to any of these miRNAs, particularly forthe treatment or prevention of cancer. Cancer includes, but is notlimited to, malignant cancers, tumors, metastatic cancers, unresectablecancers, chemo- and/or radiation-resistant cancers, and terminalcancers.

It will be understood that shorthand notations are employed such that ageneric description of a miRNA refers to any of its gene family members(distinguished by a number), unless otherwise indicated. It isunderstood by those of skill in the art that a “gene family” refers to agroup of genes having the same miRNA coding sequence. Typically, membersof a gene family are identified by a number following the initialdesignation. For example, miR-16-1 and miR-16-2 are members of themiR-16 gene family and “miR-7” refers to miR-7-1, miR-7-2 and miR-7-3.Moreover, unless otherwise indicated, a shorthand notation refers torelated miRNAs (distinguished by a letter). Thus, “let-7,” for example,refers to let-7a-1, let7-a-2, let-7b, let-7c, let-7d, let-7e, let-7f-1,and let-7f-2.” Exceptions to these shorthand notations will be otherwiseidentified.

It will be understood that the term “providing” an agent is used toinclude “administering” the agent to a patient.

In certain embodiments, methods also include targeting a miRNA tomodulate in a cell or organism. The term “targeting a miRNA to modulate”means a nucleic acid of the invention will be employed so as to modulatethe selected miRNA. In some embodiments the modulation is achieved witha synthetic or non-synthetic miRNA that corresponds to the targetedmiRNA, which effectively provides the targeted miRNA to the cell ororganism (positive modulation). In other embodiments, the modulation isachieved with a miRNA inhibitor, which effectively inhibits the targetedmiRNA in the cell or organism (negative modulation).

In some embodiments, the miRNA targeted to be modulated is a miRNA thataffects a disease, condition, or pathway. In certain embodiments, themiRNA is targeted because a treatment can be provided by negativemodulation of the targeted miRNA. In other embodiments, the miRNA istargeted because a treatment can be provided by positive modulation ofthe targeted miRNA.

In certain methods of the invention, there is a further step ofadministering the selected miRNA modulator to a cell, tissue, organ, ororganism (collectively “biological matter”) in need of treatment relatedto modulation of the targeted miRNA or in need of the physiological orbiological results discussed herein (such as with respect to aparticular cellular pathway or result like decrease in cell viability).Consequently, in some methods of the invention there is a step ofidentifying a patient in need of treatment that can be provided by themiRNA modulator(s). It is contemplated that an effective amount of amiRNA modulator can be administered in some embodiments. In particularembodiments, there is a therapeutic benefit conferred on the biologicalmatter, where a “therapeutic benefit” refers to an improvement in theone or more conditions or symptoms associated with a disease orcondition or an improvement in the prognosis, duration, or status withrespect to the disease. It is contemplated that a therapeutic benefitincludes, but is not limited to, a decrease in pain, a decrease inmorbidity, a decrease in a symptom. For example, with respect to cancer,it is contemplated that a therapeutic benefit can be inhibition of tumorgrowth, prevention of metastasis, reduction in number of metastases,inhibition of cancer cell proliferation, inhibition of cancer cellproliferation, induction of cell death in cancer cells, inhibition ofangiogenesis near cancer cells, induction of apoptosis of cancer cells,reduction in pain, reduction in risk of recurrence, induction of chemo-or radiosensitivity in cancer cells, prolongation of life, and/or delayof death directly or indirectly related to cancer.

Furthermore, it is contemplated that the miRNA compositions may beprovided as part of a therapy to a patient, in conjunction withtraditional therapies or preventative agents. Moreover, it iscontemplated that any method discussed in the context of therapy may beapplied as preventatively, particularly in a patient identified to bepotentially in need of the therapy or at risk of the condition ordisease for which a therapy is needed.

In addition, methods of the invention concern employing one or morenucleic acids corresponding to a miRNA and a therapeutic drug. Thenucleic acid can enhance the effect or efficacy of the drug, reduce anyside effects or toxicity, modify its bioavailability, and/or decreasethe dosage or frequency needed. In certain embodiments, the therapeuticdrug is a cancer therapeutic. Consequently, in some embodiments, thereis a method of treating cancer in a patient comprising administering tothe patient the cancer therapeutic and an effective amount of at leastone miRNA molecule that improves the efficacy of the cancer therapeuticor protects non-cancer cells. Cancer therapies also include a variety ofcombination therapies with both chemical and radiation based treatments.Combination chemotherapies include but are not limited to, for example,bevacizumab, cisplatin (CDDP), carboplatin, EGFR inhibitors (gefitiniband cetuximab), procarbazine, mechlorethamine, cyclophosphamide,camptothecin, COX-2 inhibitors (e.g., celecoxib) ifosfamide, melphalan,chlorambucil, busulfan, nitrosurea, dactinomycin, daunorubicin,doxorubicin (adriamycin), bleomycin, plicomycin, mitomycin, etoposide(VP16), tamoxifen, raloxifene, estrogen receptor binding agents, taxol,taxotere, gemcitabien, navelbine, farnesyl-protein transferaseinhibitors, transplatinum, 5-fluorouracil, vincristin, vinblastin andmethotrexate, or any analog or derivative variant of the foregoing.

Generally, inhibitors of miRNAs can be given to achieve the oppositeeffect as compared to when nucleic acid molecules corresponding to themature miRNA are given. Similarly, nucleic acid molecules correspondingto the mature miRNA can be given to achieve the opposite effect ascompared to when inhibitors of the miRNA are given. For example, miRNAmolecules that increase cell proliferation can be provided to cells toincrease proliferation or inhibitors of such molecules can be providedto cells to decrease cell proliferation. The present inventioncontemplates these embodiments in the context of the differentphysiological effects observed with the different miRNA molecules andmiRNA inhibitors disclosed herein. These include, but are not limitedto, the following physiological effects: increase and decreasing cellproliferation, increasing or decreasing apoptosis, increasingtransformation, increasing or decreasing cell viability, activating ERK,activating/inducing or inhibiting hTert, inhibit stimulation of Stat3,reduce or increase viable cell number, and increase or decrease numberof cells at a particular phase of the cell cycle. Methods of theinvention are generally contemplated to include providing or introducingone or more different nucleic acid molecules corresponding to one ormore different miRNA molecules. It is contemplated that the following,at least the following, or at most the following number of differentnucleic acid molecules may be provided or introduced: 1, 2, 3, 4, 5, 6,7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25,26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43,44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61,62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79,80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97,98, 99, 100, or any range derivable therein. This also applies to thenumber of different miRNA molecules that can be provided or introducedinto a cell.

1. Nucleobases

As used herein a “nucleobase” refers to a heterocyclic base, such as forexample a naturally occurring nucleobase (i.e., an A, T, G, C or U)found in at least one naturally occurring nucleic acid (i.e., DNA andRNA), and naturally or non-naturally occurring derivative(s) and analogsof such a nucleobase. A nucleobase generally can form one or morehydrogen bonds (“anneal” or “hybridize”) with at least one naturallyoccurring nucleobase in manner that may substitute for naturallyoccurring nucleobase pairing (e.g., the hydrogen bonding between A andT, G and C, and A and U).

“Purine” and/or “pyrimidine” nucleobase(s) encompass naturally occurringpurine and/or pyrimidine nucleobases and also derivative(s) andanalog(s) thereof, including but not limited to, those a purine orpyrimidine substituted by one or more of an alkyl, carboxyalkyl, amino,hydroxyl, halogen (i.e., fluoro, chloro, bromo, or iodo), thiol oralkylthiol moiety. Preferred alkyl (e.g., alkyl, carboxyalkyl, etc.)moieties comprise of from about 1, about 2, about 3, about 4, about 5,to about 6 carbon atoms. Other non-limiting examples of a purine orpyrimidine include a deazapurine, a 2,6-diaminopurine, a 5-fluorouracil,a xanthine, a hypoxanthine, a 8-bromoguanine, a 8-chloroguanine, abromothymine, a 8-aminoguanine, a 8-hydroxyguanine, a 8-methylguanine, a8-thioguanine, an azaguanine, a 2-aminopurine, a 5-ethylcytosine, a5-methylcyosine, a 5-bromouracil, a 5-ethyluracil, a 5-iodouracil, a5-chlorouracil, a 5-propyluracil, a thiouracil, a 2-methyladenine, amethylthioadenine, a N,N-diemethyladenine, an azaadenines, a8-bromoadenine, a 8-hydroxyadenine, a 6-hydroxyaminopurine, a6-thiopurine, a 4-(6-aminohexyl/cytosine), and the like. Other examplesare well known to those of skill in the art.

A nucleobase may be comprised in a nucleoside or nucleotide, using anychemical or natural synthesis method described herein or known to one ofordinary skill in the art. Such nucleobase may be labeled or it may bepart of a molecule that is labeled and contains the nucleobase.

As used herein, a “nucleoside” refers to an individual chemical unitcomprising a nucleobase covalently attached to a nucleobase linkermoiety. A non-limiting example of a “nucleobase linker moiety” is asugar comprising 5-carbon atoms (i.e., a “5-carbon sugar”), includingbut not limited to a deoxyribose, a ribose, an arabinose, or aderivative or an analog of a 5-carbon sugar. Non-limiting examples of aderivative or an analog of a 5-carbon sugar include a2′-fluoro-2′-deoxyribose or a carbocyclic sugar where a carbon issubstituted for an oxygen atom in the sugar ring. Different types ofcovalent attachment(s) of a nucleobase to a nucleobase linker moiety areknown in the art (Komberg and Baker, 1992).

As used herein, a “nucleotide” refers to a nucleoside further comprisinga “backbone moiety”. A backbone moiety generally covalently attaches anucleotide to another molecule comprising a nucleotide, or to anothernucleotide to form a nucleic acid. The “backbone moiety” in naturallyoccurring nucleotides typically comprises a phosphorus moiety, which iscovalently attached to a 5-carbon sugar. The attachment of the backbonemoiety typically occurs at either the 3′- or 5′-position of the 5-carbonsugar. However, other types of attachments are known in the art,particularly when a nucleotide comprises derivatives or analogs of anaturally occurring 5-carbon sugar or phosphorus moiety.

2. Nucleic Acid Analogs

A nucleic acid may comprise, or be composed entirely of, a derivative oranalog of a nucleobase, a nucleobase linker moiety and/or backbonemoiety that may be present in a naturally occurring nucleic acid. RNAwith nucleic acid analogs may also be labeled according to methods ofthe invention. As used herein a “derivative” refers to a chemicallymodified or altered form of a naturally occurring molecule, while theterms “mimic” or “analog” refer to a molecule that may or may notstructurally resemble a naturally occurring molecule or moiety, butpossesses similar functions. As used herein, a “moiety” generally refersto a smaller chemical or molecular component of a larger chemical ormolecular structure. Nucleobase, nucleoside and nucleotide analogs orderivatives are well known in the art, and have been described (see forexample, Scheit, 1980, incorporated herein by reference).

Additional non-limiting examples of nucleosides, nucleotides or nucleicacids comprising 5-carbon sugar and/or backbone moiety derivatives oranalogs, include those in: U.S. Pat. No. 5,681,947, which describesoligonucleotides comprising purine derivatives that form triple helixeswith and/or prevent expression of dsDNA; U.S. Pat. Nos. 5,652,099 and5,763,167, which describe nucleic acids incorporating fluorescentanalogs of nucleosides found in DNA or RNA, particularly for use asfluorescent nucleic acids probes; U.S. Pat. No. 5,614,617, whichdescribes oligonucleotide analogs with substitutions on pyrimidine ringsthat possess enhanced nuclease stability; U.S. Pat. Nos. 5,670,663,5,872,232 and 5,859,221, which describe oligonucleotide analogs withmodified 5-carbon sugars (i.e., modified 2′-deoxyfuranosyl moieties)used in nucleic acid detection; U.S. Pat. No. 5,446,137, which describesoligonucleotides comprising at least one 5-carbon sugar moietysubstituted at the 4′ position with a substituent other than hydrogenthat can be used in hybridization assays; U.S. Pat. No. 5,886,165, whichdescribes oligonucleotides with both deoxyribonucleotides with 3′-5′internucleotide linkages and ribonucleotides with 2′-5′ internucleotidelinkages; U.S. Pat. No. 5,714,606, which describes a modifiedinternucleotide linkage wherein a 3′-position oxygen of theinternucleotide linkage is replaced by a carbon to enhance the nucleaseresistance of nucleic acids; U.S. Pat. No. 5,672,697, which describesoligonucleotides containing one or more 5′ methylene phosphonateinternucleotide linkages that enhance nuclease resistance; U.S. Pat.Nos. 5,466,786 and 5,792,847, which describe the linkage of asubstituent moiety which may comprise a drug or label to the 2′ carbonof an oligonucleotide to provide enhanced nuclease stability and abilityto deliver drugs or detection moieties; U.S. Pat. No. 5,223,618, whichdescribes oligonucleotide analogs with a 2 or 3 carbon backbone linkageattaching the 4′ position and 3′ position of adjacent 5-carbon sugarmoiety to enhanced cellular uptake, resistance to nucleases andhybridization to target RNA; U.S. Pat. No. 5,470,967, which describesoligonucleotides comprising at least one sulfamate or sulfamideinternucleotide linkage that are useful as nucleic acid hybridizationprobe; U.S. Pat. Nos. 5,378,825, 5,777,092, 5,623,070, 5,610,289 and5,602,240, which describe oligonucleotides with three or four atomlinker moiety replacing phosphodiester backbone moiety used for improvednuclease resistance, cellular uptake, and regulating RNA expression;U.S. Pat. No. 5,858,988, which describes hydrophobic carrier agentattached to the 2′-O position of oligonucleotides to enhanced theirmembrane permeability and stability; U.S. Pat. No. 5,214,136, whichdescribes oligonucleotides conjugated to anthraquinone at the 5′terminus that possess enhanced hybridization to DNA or RNA; enhancedstability to nucleases; U.S. Pat. No. 5,700,922, which describesPNA-DNA-PNA chimeras wherein the DNA comprises2′-deoxy-erythro-pentofuranosyl nucleotides for enhanced nucleaseresistance, binding affinity, and ability to activate RNase H; and U.S.Pat. No. 5,708,154, which describes RNA linked to a DNA to form aDNA-RNA hybrid; U.S. Pat. No. 5,728,525, which describes the labeling ofnucleoside analogs with a universal fluorescent label.

Additional teachings for nucleoside analogs and nucleic acid analogs areU.S. Pat. No. 5,728,525, which describes nucleoside analogs that areend-labeled; U.S. Pat. Nos. 5,637,683, 6,251,666 (L-nucleotidesubstitutions), and 5,480,980 (7-deaza-2′deoxyguanosine nucleotides andnucleic acid analogs thereof).

Additional non-limiting examples of nucleosides, nucleotides or nucleicacids include

those in: U.S. Pat. Nos. 5,681,947, 5,652,099 and 5,763,167, 5,614,617,5,670,663, 5,872,232, 5,859,221, 5,446,137, 5,886,165, 5,714,606,5,672,697, 5,466,786, 5,792,847, 5,223,618, 5,470,967, 5,378,825,5,777,092, 5,623,070, 5,610,289, 5,602,240, 5,858,988, 5,214,136,5,700,922, 5,708,154, 5,728,525, 5,637,683, 6,251,666, 5,480,980, and5,728,525, each of which is incorporated herein by reference in itsentirety.

3. Modified Nucleotides

Labeling methods and kits of the invention specifically contemplate theuse of nucleotides that are both modified for attachment of a label andcan be incorporated into a miRNA molecule. Such nucleotides includethose that can be labeled with a dye, including a fluorescent dye, orwith a molecule such as biotin. Labeled nucleotides are readilyavailable; they can be acquired commercially or they can be synthesizedby reactions known to those of skill in the art.

Modified nucleotides for use in the invention may not be naturallyoccurring nucleotides, but instead, refer to prepared nucleotides thathave a reactive moiety on them. Specific reactive functionalities ofinterest include: amino, sulfhydryl, sulfoxyl, aminosulfhydryl, azido,epoxide, isothiocyanate, isocyanate, anhydride, monochlorotriazine,dichlorotriazine, mono- or dihalogen substituted pyridine, mono- ordisubstituted diazine, maleimide, epoxide, aziridine, sulfonyl halide,acid halide, alkyl halide, aryl halide, alkylsulfonate,N-hydroxysuccinimide ester, imido ester, hydrazine, azidonitrophenyl,azide, 3-(2-pyridyl dithio)-propionamide, glyoxal, aldehyde, iodoacetyl,cyanomethyl ester, p-nitrophenyl ester, o-nitrophenyl ester,hydroxypyridine ester, carbonyl imidazole, and the other such chemicalgroups. In some embodiments, the reactive functionality may be bondeddirectly to a nucleotide, or it may be bonded to the nucleotide througha linking group. The functional moiety and any linker cannotsubstantially impair the ability of the nucleotide to be added to themiRNA or to be labeled. Representative linking groups include carboncontaining linking groups, typically ranging from about 2 to 18, usuallyfrom about 2 to 8 carbon atoms, where the carbon containing linkinggroups may or may not include one or more heteroatoms, e.g. S, O, Netc., and may or may not include one or more sites of unsaturation. Ofparticular interest in many embodiments are alkyl linking groups,typically lower alkyl linking groups of 1 to 16, usually 1 to 4 carbonatoms, where the linking groups may include one or more sites ofunsaturation. The functionalized nucleotides (or primers) used in theabove methods of functionalized target generation may be fabricatedusing known protocols or purchased from commercial vendors, e.g., Sigma,Roche, Ambion, Biosearch Technologies and NEN. Functional groups may beprepared according to ways known to those of skill in the art, includingthe representative information found in U.S. Pat. Nos. 4,404,289;4,405,711; 4,337,063 and 5,268,486, and U.K. Patent 1,529,202, which areall incorporated by reference.

Amine-modified nucleotides are used in several embodiments of theinvention. The amine-modified nucleotide is a nucleotide that has areactive amine group for attachment of the label. It is contemplatedthat any ribonucleotide (G, A, U, or C) or deoxyribonucleotide (G, A, T,or C) can be modified for labeling. Examples include, but are notlimited to, the following modified ribo- and deoxyribo-nucleotides:5-(3-aminoallyl)-UTP; 8-[(4-amino)butyl]-amino-ATP and8-[(6-amino)butyl]-amino-ATP; N6-(4-amino)butyl-ATP,N6-(6-amino)butyl-ATP, N4-[2,2-oxy-bis-(ethylamine)]-CTP;N6-(6-Amino)hexyl-ATP; 8-[(6-Amino)hexyl]-amino-ATP;5-propargylamino-CTP, 5-propargylamino-UTP; 5-(3-aminoallyl)-dUTP;8-[(4-amino)butyl]-amino-dATP and 8-[(6-amino)butyl]-amino-dATP;N6-(4-amino)butyl-dATP, N6-(6-amino)butyl-dATP,N4-[2,2-oxy-bis-(ethylamine)]-dCTP; N6-(6-Amino)hexyl-dATP;8-[(6-Amino)hexyl]-amino-dATP; 5-propargylamino-dCTP, and5-propargylamino-dUTP. Such nucleotides can be prepared according tomethods known to those of skill in the art. Moreover, a person ofordinary skill in the art could prepare other nucleotide entities withthe same amine-modification, such as a 5-(3-aminoallyl)-CTP, GTP, ATP,dCTP, dGTP, dTTP, or dUTP in place of a 5-(3-aminoallyl)-UTP.

B. Preparation of Nucleic Acids

A nucleic acid may be made by any technique known to one of ordinaryskill in the art, such as for example, chemical synthesis, enzymaticproduction or biological production. It is specifically contemplatedthat miRNA probes of the invention are chemically synthesized.

In some embodiments of the invention, miRNAs are recovered or isolatedfrom a biological sample. The miRNA may be recombinant or it may benatural or endogenous to the cell (produced from the cell's genome). Itis contemplated that a biological sample may be treated in a way so asto enhance the recovery of small RNA molecules such as miRNA. U.S.patent application Ser. No. 10/667,126 describes such methods and it isspecifically incorporated by reference herein. Generally, methodsinvolve lysing cells with a solution having guanidinium and a detergent.

Alternatively, nucleic acid synthesis is performed according to standardmethods. See, for example, Itakura and Riggs (1980). Additionally, U.S.Pat. Nos. 4,704,362, 5,221,619, and 5,583,013 each describe variousmethods of preparing synthetic nucleic acids. Non-limiting examples of asynthetic nucleic acid (e.g., a synthetic oligonucleotide), include anucleic acid made by in vitro chemically synthesis usingphosphotriester, phosphite, or phosphoramidite chemistry and solid phasetechniques such as described in EP 266,032, incorporated herein byreference, or via deoxynucleoside H-phosphonate intermediates asdescribed by Froehler et al., 1986 and U.S. Pat. No. 5,705,629, eachincorporated herein by reference. In the methods of the presentinvention, one or more oligonucleotide may be used. Various differentmechanisms of oligonucleotide synthesis have been disclosed in forexample, U.S. Pat. Nos. 4,659,774, 4,816,571, 5,141,813, 5,264,566,4,959,463, 5,428,148, 5,554,744, 5,574,146, 5,602,244, each of which isincorporated herein by reference.

A non-limiting example of an enzymatically produced nucleic acid includeone produced by enzymes in amplification reactions such as PCR™ (see forexample, U.S. Pat. Nos. 4,683,202 and 4,682,195, each incorporatedherein by reference), or the synthesis of an oligonucleotide describedin U.S. Pat. No. 5,645,897, incorporated herein by reference. Anon-limiting example of a biologically produced nucleic acid includes arecombinant nucleic acid produced (i.e., replicated) in a living cell,such as a recombinant DNA vector replicated in bacteria (see forexample, Sambrook et al., 2001, incorporated herein by reference).

Oligonucleotide synthesis is well known to those of skill in the art.Various different mechanisms of oligonucleotide synthesis have beendisclosed in for example, U.S. Pat. Nos. 4,659,774, 4,816,571,5,141,813, 5,264,566, 4,959,463, 5,428,148, 5,554,744, 5,574,146,5,602,244, each of which is incorporated herein by reference.

Basically, chemical synthesis can be achieved by the diester method, thetriester method polynucleotides phosphorylase method and by solid-phasechemistry. The diester method was the first to be developed to a usablestate, primarily by Khorana and co-workers. (Khorana, 1979). The basicstep is the joining of two suitably protected deoxynucleotides to form adideoxynucleotide containing a phosphodiester bond.

The main difference between the diester and triester methods is thepresence in the latter of an extra protecting group on the phosphateatoms of the reactants and products (Itakura et al, 1975).Purification's are typically done in chloroform solutions. Otherimprovements in the method include (i) the block coupling of trimers andlarger oligomers, (ii) the extensive use of high-performance liquidchromatography for the purification of both intermediate and finalproducts, and (iii) solid-phase synthesis.

Recombinant methods for producing nucleic acids in a cell are well knownto those of skill in the art. These include the use of vectors (viraland non-viral), plasmids, cosmids, and other vehicles for delivering anucleic acid to a cell, which may be the target cell (e.g., a cancercell) or simply a host cell (to produce large quantities of the desiredRNA molecule). Alternatively, such vehicles can be used in the contextof a cell free system so long as the reagents for generating the RNAmolecule are present. Such methods include those described in Sambrook,2003, Sambrook, 2001 and Sambrook, 1989, which are hereby incorporatedby reference.

In certain embodiments, the present invention concerns nucleic acidmolecules that are not synthetic. In some embodiments, the nucleic acidmolecule has a chemical structure of a naturally occurring nucleic acidand a sequence of a naturally occurring nucleic acid, such as the exactand entire sequence of a single stranded primary miRNA (see Lee 2002), asingle-stranded precursor miRNA, or a single-stranded mature miRNA. Inaddition to the use of recombinant technology, such non-syntheticnucleic acids may be generated chemically, such as by employingtechnology used for creating oligonucleotides.

C. Isolation of Nucleic Acids

Nucleic acids may be isolated using techniques well known to those ofskill in the art, though in particular embodiments, methods forisolating small nucleic acid molecules, and/or isolating RNA moleculescan be employed. Chromatography is a process often used to separate orisolate nucleic acids from protein or from other nucleic acids. Suchmethods can involve electrophoresis with a gel matrix, filter columns,alcohol precipitation, and/or other chromatography. If miRNA from cellsis to be used or evaluated, methods generally involve lysing the cellswith a chaotropic (e.g., guanidinium isothiocyanate) and/or detergent(e.g., N-lauroyl sarcosine) prior to implementing processes forisolating particular populations of RNA.

In particular methods for separating miRNA from other nucleic acids, agel matrix is prepared using polyacrylamide, though agarose can also beused. The gels may be graded by concentration or they may be uniform.Plates or tubing can be used to hold the gel matrix for electrophoresis.Usually one-dimensional electrophoresis is employed for the separationof nucleic acids. Plates are used to prepare a slab gel, while thetubing (glass or rubber, typically) can be used to prepare a tube gel.The phrase “tube electrophoresis” refers to the use of a tube or tubing,instead of plates, to form the gel. Materials for implementing tubeelectrophoresis can be readily prepared by a person of skill in the artor purchased, such as from C.B.S. Scientific Co., Inc. or Scie-Plas.

Methods may involve the use of organic solvents and/or alcohol toisolate nucleic acids, particularly miRNA used in methods andcompositions of the invention. Some embodiments are described in U.S.patent application Ser. No. 10/667,126, which is hereby incorporated byreference. Generally, this disclosure provides methods for efficientlyisolating small RNA molecules from cells comprising: adding an alcoholsolution to a cell lysate and applying the alcohol/lysate mixture to asolid support before eluting the RNA molecules from the solid support.In some embodiments, the amount of alcohol added to a cell lysateachieves an alcohol concentration of about 55% to 60%. While differentalcohols can be employed, ethanol works well. A solid support may be anystructure, and it includes beads, filters, and columns, which mayinclude a mineral or polymer support with electronegative groups. Aglass fiber filter or column has worked particularly well for suchisolation procedures.

In specific embodiments, miRNA isolation processes include: (a) lysingcells in the sample with a lysing solution comprising guanidinium,wherein a lysate with a concentration of at least about 1 M guanidiniumis produced; (b) extracting miRNA molecules from the lysate with anextraction solution comprising phenol; (c) adding to the lysate analcohol solution for form a lysate/alcohol mixture, wherein theconcentration of alcohol in the mixture is between about 35% to about70%; (d) applying the lysate/alcohol mixture to a solid support; (e)eluting the miRNA molecules from the solid support with an ionicsolution; and, (f) capturing the miRNA molecules. Typically the sampleis dried down and resuspended in a liquid and volume appropriate forsubsequent manipulation.

II. Labels and Labeling Techniques

In some embodiments, the present invention concerns miRNA or mRNA thatare labeled. It is contemplated that miRNA or mRNA may first be isolatedand/or purified prior to labeling. This may achieve a reaction that moreefficiently labels the miRNA, as opposed to other RNA in a sample inwhich the miRNA is not isolated or purified prior to labeling. In manyembodiments of the invention, the label is non-radioactive. Generally,nucleic acids may be labeled by adding labeled nucleotides (one-stepprocess) or adding nucleotides and labeling the added nucleotides(two-step process).

A. Labeling Techniques

In some embodiments, nucleic acids are labeled by catalytically addingto the nucleic acid an already labeled nucleotide or nucleotides. One ormore labeled nucleotides can be added to miRNA molecules. See U.S. Pat.No. 6,723,509, which is hereby incorporated by reference.

In other embodiments, an unlabeled nucleotide or nucleotides iscatalytically added to a miRNA, and the unlabeled nucleotide is modifiedwith a chemical moiety that enables it to be subsequently labeled. Inembodiments of the invention, the chemical moiety is a reactive aminesuch that the nucleotide is an amine-modified nucleotide. Examples ofamine-modified nucleotides are well known to those of skill in the art,many being commercially available such as from Ambion, Sigma, JenaBioscience, and TriLink.

In contrast to labeling of cDNA during its synthesis, the issue forlabeling miRNA is how to label the already existing molecule. An enzymeusing a di- or tri-phosphate ribonucleotide or deoxyribonucleotide as asubstrate for its addition to a miRNA can be used. Moreover, in specificembodiments, it involves using a modified di- or tri-phosphateribonucleotide, which is added to the 3′ end of a miRNA. Examples ofsources for the enzymes include yeast, gram-negative bacteria such as E.coli, lactococcus lactis, and sheep pox virus. Enzymes capable of addingsuch nucleotides include, but are not limited to, poly(A) polymerase,terminal transferase, and polynucleotide phosphorylase. In specificembodiments of the invention, a ligase is contemplated as not being theenzyme used to add the label, and instead, a non-ligase enzyme isemployed.

B. Labels

Labels on miRNA, miRNA probes, or RNA probes may be colorimetric(includes visible and UV spectrum, including fluorescent), luminescent,enzymatic, or positron emitting (including radioactive). The label maybe detected directly or indirectly. Radioactive labels include ¹²⁵I,³²P, ³³P, and ³⁵S. Examples of enzymatic labels include alkalinephosphatase, luciferase, horseradish peroxidase, and β-galactosidase.Labels can also be proteins with luminescent properties, e.g., greenfluorescent protein and phycoerythrin.

The colorimetric and fluorescent labels contemplated for use asconjugates include, but are not limited to, Alexa Fluor dyes, BODIPYdyes, such as BODIPY FL; Cascade Blue; Cascade Yellow; coumarin and itsderivatives, such as 7-amino-4-methylcoumarin, aminocoumarin andhydroxycoumarin; cyanine dyes, such as Cy3 and Cy5; eosins anderythrosins; fluorescein and its derivatives, such as fluoresceinisothiocyanate; macrocyclic chelates of lanthanide ions, such as QuantumDye™; Marina Blue; Oregon Green; rhodamine dyes, such as rhodamine red,tetramethylrhodamine and rhodamine 6G; Texas Red; fluorescent energytransfer dyes, such as thiazole orange-ethidium heterodimer; and, TOTAB.

Specific examples of dyes include, but are not limited to, thoseidentified above and the following: Alexa Fluor 350, Alexa Fluor 405,Alexa Fluor 430, Alexa Fluor 488, Alexa Fluor 500. Alexa Fluor 514,Alexa Fluor 532, Alexa Fluor 546, Alexa Fluor 555, Alexa Fluor 568,Alexa Fluor 594, Alexa Fluor 610, Alexa Fluor 633, Alexa Fluor 647,Alexa Fluor 660, Alexa Fluor 680, Alexa Fluor 700, and, Alexa Fluor 750;amine-reactive BODIPY dyes, such as BODIPY 493/503, BODIPY 530/550,BODIPY 558/568, BODIPY 564/570, BODIPY 576/589, BODIPY 581/591, BODIPY630/650, BODIPY 650/655, BODIPY FL, BODIPY R6G, BODIPY TMR, and,BODIPY-TR; Cy3, Cy5, 6-FAM, Fluorescein Isothiocyanate, HEX, 6-JOE,Oregon Green 488, Oregon Green 500, Oregon Green 514, Pacific Blue, REG,Rhodamine Green, Rhodamine Red, Renographin, ROX, SYPRO, TAMPA,2′,4′,5′,7′-Tetrabromosulfonefluorescein, and TET.

Specific examples of fluorescently labeled ribonucleotides are availablefrom Molecular Probes, and these include, Alexa Fluor 488-5-UTP,Fluorescein-12-UTP, BODIPY FL-14-UTP, BODIPY TMR-14-UTP,Tetramethylrhodamine-6-UTP, Alexa Fluor 546-14-UTP, Texas Red-5-UTP, andBODIPY TR-14-UTP. Other fluorescent ribonucleotides are available fromAmersham Biosciences, such as Cy3-UTP and Cy5-UTP.

Examples of fluorescently labeled deoxyribonucleotides includeDinitrophenyl (DNP)-11-dUTP, Cascade Blue-7-dUTP, Alexa Fluor488-5-dUTP, Fluorescein-12-dUTP, Oregon Green 488-5-dUTP, BODIPYFL-14-dUTP, Rhodamine Green-5-dUTP, Alexa Fluor 532-5-dUTP, BODIPYTMR-14-dUTP, Tetramethylrhodamine-6-dUTP, Alexa Fluor 546-14-dUTP, AlexaFluor 568-5-dUTP, Texas Red-12-dUTP, Texas Red-5-dUTP, BODIPYTR-14-dUTP, Alexa Fluor 594-5-dUTP, BODIPY 630/650-14-dUTP, BODIPY650/665-14-dUTP; Alexa Fluor 488-7-OBEA-dCTP, Alexa Fluor546-16-OBEA-dCTP, Alexa Fluor 594-7-OBEA-dCTP, Alexa Fluor647-12-OBEA-dCTP.

It is contemplated that nucleic acids may be labeled with two differentlabels. Furthermore, fluorescence resonance energy transfer (FRET) maybe employed in methods of the invention (e.g., Klostermeier et al.,2002; Emptage, 2001; Didenko, 2001, each incorporated by reference).

Alternatively, the label may not be detectable per se, but indirectlydetectable or allowing for the isolation or separation of the targetednucleic acid. For example, the label could be biotin, digoxigenin,polyvalent cations, chelator groups and the other ligands, includeligands for an antibody.

C. Visualization Techniques

A number of techniques for visualizing or detecting labeled nucleicacids are readily available. Such techniques include, microscopy,arrays, Fluorometry, Light cyclers or other real time PCR machines, FACSanalysis, scintillation counters, Phosphoimagers, Geiger counters, MRI,CAT, antibody-based detection methods (Westerns, immunofluorescence,immunohistochemistry), histochemical techniques, HPLC (Griffey et al.,1997), spectroscopy, capillary gel electrophoresis (Cummins et al.,1996), spectroscopy; mass spectroscopy; radiological techniques; andmass balance techniques.

When two or more differentially colored labels are employed, fluorescentresonance energy transfer (FRET) techniques may be employed tocharacterize association of one or more nucleic acid. Furthermore, aperson of ordinary skill in the art is well aware of ways ofvisualizing, identifying, and characterizing labeled nucleic acids, andaccordingly, such protocols may be used as part of the invention.Examples of tools that may be used also include fluorescent microscopy,a BioAnalyzer, a plate reader, Storm (Molecular Dynamics), ArrayScanner, FACS (fluorescent activated cell sorter), or any instrumentthat has the ability to excite and detect a fluorescent molecule.

III. Array Preparation and Screening

Nucleic acids of the invention include one or more nucleic acidcomprising at least one segment having a sequence or complementarysequence of one or more of the miRNA sequences described herein. Nucleicacid probes of the invention are typically coupled to a support. Suchsupports are well known to those of ordinary skill in the art andinclude, but are not limited to glass, plastic, metal, or latex. Inparticular aspects of the invention, the support can be planar or in theform of a bead or other geometric shapes or configurations.

A. Array Preparation

Certain embodiments of the present invention concerns the preparationand use of mRNA or nucleic acid arrays, miRNA or nucleic acid arrays,and/or miRNA or nucleic acid probe arrays, which are macroarrays ormicroarrays of nucleic acid molecules (probes) that are fully or nearlycomplementary (over the length of the prove) or identical (over thelength of the prove) to a plurality of nucleic acid, mRNA or miRNAmolecules, precursor miRNA molecules, or nucleic acids derived from thevarious genes and gene pathways modulated by miR-10 miRNAs and that arepositioned on a support or support material in a spatially separatedorganization. Macroarrays are typically sheets of nitrocellulose ornylon upon which probes have been spotted. Microarrays position thenucleic acid probes more densely such that up to 10,000 nucleic acidmolecules can be fit into a region typically 1 to 4 square centimeters.Microarrays can be fabricated by spotting nucleic acid molecules, e.g.,genes, oligonucleotides, etc., onto substrates or fabricatingoligonucleotide sequences in situ on a substrate. Spotted or fabricatednucleic acid molecules can be applied in a high density matrix patternof up to about 30 non-identical nucleic acid molecules per squarecentimeter or higher, e.g. up to about 100 or even 1000 per squarecentimeter. Microarrays typically use coated glass as the solid support,in contrast to the nitrocellulose-based material of filter arrays. Byhaving an ordered array of marker RNA and/or miRNA-complementing nucleicacid samples, the position of each sample can be tracked and linked tothe original sample.

A variety of different array devices in which a plurality of distinctnucleic acid probes are stably associated with the surface of a solidsupport are known to those of skill in the art. Useful substrates forarrays include nylon, glass, metal, plastic, latex, and silicon. Sucharrays may vary in a number of different ways, including average probelength, sequence or types of probes, nature of bond between the probeand the array surface, e.g. covalent or non-covalent, and the like. Thelabeling and screening methods of the present invention and the arraysare not limited in its utility with respect to any parameter except thatthe probes detect miRNA, or genes or nucleic acid representative ofgenes; consequently, methods and compositions may be used with a varietyof different types of nucleic acid arrays.

miRNA arrays or miRNA probe arrays are ordered macroarrays ormicroarrays of nucleic acid molecules (probes) that are fully or nearlycomplementary or identical to a plurality of miRNA molecules, precursormiRNA molecules, or segments thereof and that are positioned on asupport or support material in a spatially separated organization.Representative methods and apparatus for preparing a microarray havebeen described, for example, in U.S. Pat. Nos. 5,143,854; 5,202,231;5,242,974; 5,288,644; 5,324,633; 5,384,261; 5,405,783; 5,412,087;5,424,186; 5,429,807; 5,432,049; 5,436,327; 5,445,934; 5,468,613;5,470,710; 5,472,672; 5,492,806; 5,525,464; 5,503,980; 5,510,270;5,525,464; 5,527,681; 5,529,756; 5,532,128; 5,545,531; 5,547,839;5,554,501; 5,556,752; 5,561,071; 5,571,639; 5,580,726; 5,580,732;5,593,839; 5,599,695; 5,599,672; 5,610,287; 5,624,711; 5,631,134;5,639,603; 5,654,413; 5,658,734; 5,661,028; 5,665,547; 5,667,972;5,695,940; 5,700,637; 5,744,305; 5,800,992; 5,807,522; 5,830,645;5,837,196; 5,871,928; 5,847,219; 5,876,932; 5,919,626; 6,004,755;6,087,102; 6,368,799; 6,383,749; 6,617,112; 6,638,717; 6,720,138, aswell as WO 93/17126; WO 95/11995; WO 95/21265; WO 95/21944; WO 95/35505;WO 96/31622; WO 97/10365; WO 97/27317; WO 99/35505; WO 09923256; WO09936760; WO0138580; WO 0168255; WO 03020898; WO 03040410; WO 03053586;WO 03087297; WO 03091426; WO03100012; WO 04020085; WO 04027093; EP 373203; EP 785 280; EP 799 897 and UK 8 803 000; the disclosures of whichare all herein incorporated by reference.

It is contemplated that the arrays contain 2, 20, 25, 50, 80, 100 ormore different probes. It is contemplated that they may contain 1000,16,000, 65,000, 250,000 or 1,000,000 or more different probes. Theprobes can be directed to targets in one or more different organisms orcell types. The oligonucleotide probes range from 5 to 50, 5 to 45, 10to 40, 9 to 34, or 15 to 40 nucleotides in length in some embodiments.In certain embodiments, the oligonucleotide probes are 5, 10, 15, 20 to20, 25, 30, 35, 40 nucleotides in length including all integers andranges there between.

The location and sequence of each different probe sequence in the arrayare generally known. Moreover, the large number of different probes canoccupy a relatively small area providing a high density array having aprobe density of generally greater than about 60, 100, 600, 1000, 5,000,10,000, 40,000, 100,000, or 400,000 different oligonucleotide probes percm². The surface area of the array can be about or less than about 1,1.6, 2, 3, 4, 5, 6, 7, 8, 9, or 10 cm².

Moreover, a person of ordinary skill in the art could readily analyzedata generated using an array. Such protocols are disclosed above, andinclude information found in WO 9743450; WO 03023058; WO 03022421; WO03029485; WO 03067217; WO 03066906; WO 03076928; WO 03093810; WO03100448A1, all of which are specifically incorporated by reference.

B. Sample Preparation

It is contemplated that the miRNA of a wide variety of samples can beanalyzed using the arrays, index of miRNA probes, or array technology ofthe invention. Samples may be biological samples, in which case, theycan be from biopsy, fine needle aspirates, exfoliates, blood, tissue,organs, semen, saliva, tears, other bodily fluid, hair follicles, skin,lymph nodes (e.g., sentinel, proximal to a disease site, or distal to adisease site) or any sample containing or constituting biological cells.In certain aspects samples may be, but are nt limted to biopsy or cellspurified or enriched to some extent from a biopsy or other bodily fluidsor tissues. In certain embodiments, samples may be, but are not limitedto, fresh, frozen, fixed, formalin fixed, paraffin embedded, or formalinfixed and paraffin embedded. Alternatively, the sample may not be abiological sample, but be a chemical mixture, such as a cell-freereaction mixture (which may contain one or more biological enzymes).While assessment of endogenous miRNA is contemplated in the methodsdescribed herein, recombinant or synthetic miRNA may also be used invarious aspects of the invention—including therapy or as standards orreferences.

C. Hybridization

After an array or a set of miRNA probes is prepared and the miRNA in thesample is labeled, the population of target nucleic acids is contactedwith the array or probes under hybridization conditions, where suchconditions can be adjusted, as desired, to provide for an optimum levelof specificity in view of the particular assay being performed. Suitablehybridization conditions are well known to those of skill in the art andreviewed in Sambrook et al. (2001) and WO 95/21944. Of particularinterest in many embodiments is the use of stringent conditions duringhybridization. Stringent conditions are known to those of skill in theart.

It is specifically contemplated that a single array or set of probes maybe contacted with multiple samples. The samples may be labeled withdifferent labels to distinguish the samples. For example, a single arraycan be contacted with a tumor tissue sample labeled with Cy3, and normaltissue sample labeled with Cy5. Differences between the samples forparticular miRNAs corresponding to probes on the array can be readilyascertained and quantified.

The small surface area of the array permits uniform hybridizationconditions, such as temperature regulation and salt content. Moreover,because of the small area occupied by the high density arrays,hybridization may be carried out in extremely small fluid volumes (e.g.,about 250 μl or less, including volumes of about or less than about 5,10, 25, 50, 60, 70, 80, 90, 100 μl, or any range derivable therein). Insmall volumes, hybridization may proceed very rapidly.

D. Differential Expression Analyses

Arrays of the invention can be used to detect differences between twosamples. Specifically contemplated applications include identifyingand/or quantifying differences between miRNA from a sample that isnormal and from a sample that is not normal, between a cancerouscondition and a non-cancerous condition, or between two differentlytreated samples. Also, miRNA may be compared between a sample believedto be susceptible to a particular disease or condition and one believedto be not susceptible or resistant to that disease or condition. Asample that is not normal is one exhibiting phenotypic trait(s) of adisease or condition or one believed to be not normal with respect tothat disease or condition. It may be compared to a cell that is normalwith respect to that disease or condition. Phenotypic traits includesymptoms of, or susceptibility to, a disease or condition of which acomponent is or may or may not be genetic or caused by ahyperproliferative or neoplastic cell or cells.

An array comprises a solid support with nucleic acid probes attached tothe support. Arrays typically comprise a plurality of different nucleicacid probes that are coupled to a surface of a substrate in different,known locations. These arrays, also described as “microarrays” orcolloquially “chips” have been generally described in the art, forexample, U.S. Pat. Nos. 5,143,854, 5,445,934, 5,744,305, 5,677,195,6,040,193, 5,424,186 and Fodor et al., 1991), each of which isincorporated by reference in its entirety for all purposes. Techniquesfor the synthesis of these arrays using mechanical synthesis methods aredescribed in, e.g., U.S. Pat. No. 5,384,261, incorporated herein byreference in its entirety for all purposes. Although a planar arraysurface is used in certain aspects, the array may be fabricated on asurface of virtually any shape or even a multiplicity of surfaces.Arrays may be nucleic acids on beads, gels, polymeric surfaces, fiberssuch as fiber optics, glass or any other appropriate substrate, see U.S.Pat. Nos. 5,770,358, 5,789,162, 5,708,153, 6,040,193 and 5,800,992,which are hereby incorporated in their entirety for all purposes.

Tissues and/or corresponding cancer cells that may be evaluated bymethods and compositions of the invention include bladder, blood, bone,bone marrow, brain, breast, colon, esophagus, gastrointestine, gum,head, kidney, liver, lung, lymph node, nasopharynx, neck, ovary,pancreas, prostate, skin, small intesting, spleen, stomach, testis,thymus, thyroid, tongue, trachea, or uterus. Moreover, miRNA can beevaluated in precancers, such as metaplasia, dysplasia, and hyperplasia.The methods of the invention can detect metastasis and can be used indetecting, prognosing, and/or staging a patient's disease. It isspecifically contemplated that the invention can be used to evaluatedifferences between stages of disease, such as between hyperplasia,neoplasia, pre-cancer and cancer, or between a primary tumor and ametastasized tumor.

In certain embodiments, miRNA profiles may be generated to evaluate andcorrelate those profiles with pharmacokinetics. For example, miRNAprofiles may be created and evaluated for patient tumor and bloodsamples prior to the patient's being treated or during treatment todetermine if there are miRNAs whose expression correlates with theoutcome of treatment. Identification of differential miRNAs can lead toa prognostic and/or diagnostic assay that can be used to evaluate tumorand/or blood samples to determine what drug regimen the patient shouldbe provided. In addition, it can be used to identify or select patientssuitable for a particular clinical trial. If a miRNA profile isdetermined to be correlated with drug efficacy or drug toxicity that maybe relevant to whether that patient is an appropriate patient forreceiving the drug or for a particular dosage of the drug.

In addition to the above prognostic assay, blood samples from patientswith a variety of diseases can be evaluated to determine if differentdiseases can be identified based on blood miRNA levels. A diagnosticassay can be created based on the profiles that doctors can use toidentify individuals with a disease or who are at risk to develop adisease or experience a recurrence of disease.

E. Other Assays

In addition to the use of arrays and microarrays, it is contemplatedthat a number of difference assays could be employed to analyze miRNAs,their activities, and their effects. Such assays include, but are notlimited to, nucleic amplification, polymerase chain reaction,quantitative PCR, RT-PCR, in situ hybridization, Northern hybridization,hybridization protection assay (HPA)(GenProbe), branched DNA (bDNA)assay (Chiron), rolling circle amplification (RCA), single moleculehybridization detection (US Genomics), Invader assay (ThirdWaveTechnologies), and/or Bridge Litigation Assay (Genaco).

IV. Kits

Any of the compositions described herein may be comprised in a kit. In anon-limiting example, reagents for isolating miRNA, labeling miRNA,and/or evaluating a miRNA population using an array, nucleic acidamplification, and/or hybridization can be included in a kit, as wellreagents for preparation of samples from patient samples. The kit mayfurther include miRNA, miRNA probes, or reagents for creating orsynthesizing miRNA probes. The kits will thus comprise, in suitablecontainer means, an enzyme for labeling the miRNA by incorporatinglabeled nucleotide or unlabeled nucleotides that are subsequentlylabeled. In certain aspects, the kit can include amplification reagents.In other aspects, the kit may include various supports, such as glass,nylon, polymeric beads, and the like, having probes coupled there toand/or reagents for coupling any probes and/or target nucleic acids. Itmay also include one or more buffers, such as reaction buffer, labelingbuffer, washing buffer, or a hybridization buffer, compounds forpreparing the miRNA probes, and components for isolating miRNA. Otherkits of the invention may include components for making a nucleic acidarray comprising miRNA.

Kits for implementing methods of the invention described herein arespecifically contemplated. In some embodiments, there are kits forpreparing miRNA for multi-labeling and kits for preparing miRNA probesand/or miRNA arrays. In these embodiments, kit comprise, in suitablecontainer means, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 or more of thefollowing: (1) poly(A) polymerase; (2) unmodified nucleotides (G, A, T,C, and/or U); (3) a modified nucleotide (labeled or unlabeled); (4)poly(A) polymerase buffer; and, (5) at least one microfilter; (6) labelthat can be attached to a nucleotide; (7) at least one miRNA probe; (8)reaction buffer; (9) a miRNA array or components for making such anarray; (10) acetic acid; (11) alcohol; and/or (12) solutions forpreparing, isolating, enriching, and purifying miRNAs or miRNA probes orarrays. Other reagents include those generally used for manipulatingRNA, such as formamide, loading dye, ribonuclease inhibitors, and DNase.

In specific embodiments, kits of the invention include an arraycontaining miRNA probes, as described in the application. An array mayhave probes corresponding to all known miRNAs of an organism or aparticular tissue or organ in particular conditions, or to a subset ofsuch probes. The subset of probes on arrays of the invention may be orinclude those identified as relevant to a particular diagnostic,therapeutic, prognostic or staging application. For example, the arraymay contain one or more probes that is indicative or suggestive of (1) adisease or condition, (2) susceptibility or resistance to a particulardrug or treatment; (3) susceptibility to toxicity from a drug orsubstance; (4) the stage of development or severity of a disease orcondition (prognosis); and (5) genetic predisposition to a disease orcondition, or combinations thereof.

For any kit embodiment, including an array, there can be nucleic acidmolecules that contain or can be used to amplify a sequence that is avariant of, identical to or complementary to all or part of any of miRNAdescribed or named herein. In certain embodiments, a kit or array of theinvention can contain one or more probes for the miRNAs identified. Anynucleic acid discussed above may be implemented as part of a kit.

The components of the kits may be packaged either in aqueous media or inlyophilized form. The container means of the kits will generally includeat least one vial, test tube, flask, bottle, syringe or other containermeans, into which a component may be placed, and preferably, suitablyaliquoted. Where there is more than one component in the kit (labelingreagent and label may be packaged together), the kit also will generallycontain a second, third or other additional container into which theadditional components may be separately placed. However, variouscombinations of components may be comprised in a vial.

When the components of the kit are provided in one and/or more liquidsolutions, the liquid solution is an aqueous solution, with a sterileaqueous solution being particularly preferred.

However, the components of the kit may be provided as dried orlyophilized powder(s). When reagents and/or components are provided as adry powder, the powder can be reconstituted by the addition of asuitable solvent. It is envisioned that the solvent may also be providedin another container means. In some embodiments, labeling dyes areprovided as a dried power. It is contemplated that 10, 20, 30, 40, 50,60, 70, 80, 90, 100, 120, 120, 130, 140, 150, 160, 170, 180, 190, 200,300, 400, 500, 600, 700, 800, 900, 1000 μg or at least or at most thoseamounts of dried dye are provided in kits of the invention. The dye maythen be resuspended in any suitable solvent, such as DMSO.

The container means will generally include at least one vial, test tube,flask, bottle, syringe and/or other container means, into which thenucleic acid formulations are placed, preferably, suitably allocated.The kits may also comprise a second container means for containing asterile, pharmaceutically acceptable buffer and/or other diluent.

The kits of the present invention will also typically include a meansfor containing the vials in close confinement for commercial sale, suchas, e.g., injection and/or blow-molded plastic containers into which thedesired vials are retained.

Such kits may also include components that facilitate isolation of thelabeled miRNA. It may also include components that preserve or maintainthe miRNA or that protect against its degradation. Such components maybe RNAse-free or protect against RNAses. Such kits generally willcomprise, in suitable means, distinct containers for each individualreagent or solution.

A kit will also include instructions for employing the kit components aswell the use of any other reagent not included in the kit. Instructionsmay include variations that can be implemented.

Kits of the invention may also include one or more of the following:Control RNA; nuclease-free water; RNase-free containers, such as 1.5 mltubes; RNase-free elution tubes; PEG or dextran; ethanol; acetic acid;sodium acetate; ammonium acetate; guanidinium; detergent; nucleic acidsize marker; RNase-free tube tips; and RNase or DNase inhibitors.

It is contemplated that such reagents are embodiments of kits of theinvention. Such kits, however, are not limited to the particular itemsidentified above and may include any reagent used for the manipulationor characterization of miRNA.

V. EXAMPLES

The following examples are given for the purpose of illustrating variousembodiments of the invention and are not meant to limit the presentinvention in any fashion. One skilled in the art will appreciate readilythat the present invention is well adapted to carry out the objects andobtain the ends and advantages mentioned, as well as those objects, endsand advantages inherent herein. The present examples, along with themethods described herein are presently representative of preferredembodiments, are exemplary, and are not intended as limitations on thescope of the invention. Changes therein and other uses which areencompassed within the spirit of the invention as defined by the scopeof the claims will occur to those skilled in the art.

Example 1 Cervix Tissue Samples and RNA from Cervical Specimens

Total RNA, purified from four separate normal human cervixes (NCX), waspurchased from Ambion (FirstChoice® Human Cervix Total RNA, cat. no.AM6992; Ambion, Austin, Tex., USA;) (Table 1). Nine, separate squamouscell carcinoma, frozen tissue samples (TUM) collected from human uterinecervixes were purchased from ProteoGenex (ProteoGenex, Inc.; CulverCity, Calif., USA) (Table 2). Total RNA isolation from the TUM tissuesamples was performed using the mirVana™ miRNA Isolation Kit (Ambion;cat. no. AM1560), following the manufacturer's protocol.

TABLE 1 Patient information for normal cervix (NCX) tissue samples.Patient Patient HPV16/18 Sample Age Ethnicity Status NCX3 72 CaucasianNegative NCX10 56 Caucasian Negative NCX13 57 Caucasian Negative NCX1753 Caucasian N/A HPV, human papillomavirus

TABLE 2 Patient and tissue sample pathology information for ninesquamous cell carcinoma tissue samples (TUM) from human uterinecervixes. Histological Diagnosis refers to diagnosis of the cervixtissue sample. TNM Stage Age Patient (Sobin and HPV16/18 Sample (yr)Ethnicity Histological Diagnosis Wittekind, 2002) Status TUM1 52Caucasian SCC T1N1M0 16 TUM2 32 Caucasian SCC, poorly differentiatedT1bN0M0 18 TUM3 47 Caucasian Non-keratinizing SCC T1aN1M0 16 TUM4 45Caucasian SCC, moderately differentiated T1aN1M0 16 TUM5 29 CaucasianSCC, poorly differentiated T1a2N0M0 18 TUM6 55 Caucasian SCC, moderatelydifferentiated T3aN1M0 Negative TUM7 40 Caucasian SCC, moderatelydifferentiated T2aN1M0 16 + 18 TUM8 44 Caucasian SCC, well to poorlydifferentiated T1a2N0M0 16 TUM9 39 Caucasian SCC, poorly differentiatedT3aN0M0 18 SCC, squamous cell carcinoma; HPV, human papillomavirus

In addition, formalin-fixed, paraffin-embedded (FFPE) tissue samples,prepared from four cancer-positive and four cancer-negative inguinallymph nodes from eight different cervical cancer patients, werepurchased from ProteoGenex (Table 3). All tissue samples were less than2 years old. A hematoxilin-eosin (H&E)-stained slide from each samplewas reviewed by a pathologist at Asuragen to confirm lymph node statusesas cancer cell-positive (LNpos) or cancer cell-negative (LNneg).

TABLE 3 Patient and tissue sample pathology information for inguinallymph nodes (LN) from cervical cancer patients. LNs from four patientswere cancer cell-positive (LNpos), and LNs from four patients werecancer cell-negative (LNneg). Sample Age (yr) Cancer Cervix Tumor AJCCID Ethnicity Diagnosis Histological Diagnosis TNM Stage Stage LN MetsLNpos1 36 Caucasian metastatic SCC moderately to poorly differentiatedT1b1N1M0 IIIB 3/6 LNpos2 47 Caucasian metastatic SCC poorlydifferentiated T1b1N1M0 IIIB 3/3 LNpos3 54 Caucasian metastatic SCCmoderately to poorly differentiated T1b1N1M0 IIIB 3/5 LNpos4 34Caucasian metastatic SCC moderately differentiated T1b1N1M0 IIIB 3/5LNneg1 40 Caucasian Normal SCC moderately differentiated T1b1N0M0 IB10/6 LNneg2 52 Caucasian Normal SCC moderately differentiated T1b1N0M0IB1 0/3 LNneg3 39 Caucasian Normal SCC moderately differentiatedT1b1N0M0 IB1 0/9 LNneg4 56 Caucasian Normal SCC moderatelydifferentiated T2aN0M0 IIA  0/10 SCC, squamous cell carcinoma; TNM stage(Sobin and Wittekind, 2002); AJCC Stage (Greene, 2002).

Total RNA was extracted from FFPE tissue samples using a RecoverAll™ kit(Ambion; RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE, catno.AM1975), using the manufacturer's protocol as modified in Doleshal etal.). Purified total RNA was quantified using a Nanodrop® ND-1000(Nanoprop Technologies, Wilmington, Del., USA).

Example 2 miRNAs Differentally Expressed Between Normal Cervix Tissueand Cancer-negative Inguinal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis of cervicalcancer to inguinal lymph nodes, miRNAs were identified that areexpressed at significantly higher levels in normal cervix tissue than incancer-negative inguinal lymph nodes.

miRNA expression was determined for four normal cervix tissue samples(NCX3, NCX10, NCX13, NCX17— described in Example 1, Table 1 above) andfour cancer-negative lymph nodes (LNneg1-4—described in Example 1, Table3 above). For each tissue sample, microRNA-containing fractions werepurified from 10 μg of total RNA using a flashPAGE™ FractionationApparatus (Ambion). Purified small RNAs were enzymatically biotinylatedat their 3′-termini and hybridized to a custom-made microRNA array(miRChip V1.0, manufactured by Affymetrix, Santa Clara, Calif., USA forAmbion) by DiscovArray™ miRNA Expression Service (Asuragen). The miRChipV1.0 contains probes for more than 13,000 verified and candidate miRNAsfrom a number of sources including Sanger miRBase v9.2 database(Griffiths-Jones et al., 2006) and published reports (Bentwich et al.,2005; Berezikov et al., 2005; Xie et al., 2005; Cummins et al., 2006).Hybridization, washing, staining, imaging, and signal extraction wereperformed as recommended by the miRChip manufacturer, except that the20× GeneChip® Eukaryotic Hybridization Control cocktail was omitted.

Signal processing with the miRChip is a multi-step process involvingprobe-specific signal detection calls, background estimate andcorrection, constant variance stabilization and either array scaling orglobal normalization. For an overview of miRNA processing and analysissee Davison et al., (2006).

Probe specific signal detection calls. Each probe on an array is assayedfor detection based on a Wilcoxon rank-sum test of the miRNA probesignal compared to the distribution of signals from GC-content-matched,anti-genomic probes. If the resulting p-value for the probes is ≦0.06then it is considered “detected above background.” Probes withp-values >0.06 have insufficient signal to discriminate from thebackground and are thus considered not detected.

Background estimate and correction. The same set of anti-genomic probesused to determine detection calls are used to estimateGC-content-matched background signals. Each miRNA probe signal has aGC-content-matched background estimate subtracted from its value. ThisGC-specific background contribution is estimated by the median signalfrom the distribution of GC-matched, anti-genomic probes.

Constant variance stabilization. Probes with low signal often exhibit ahigh degree of variability once transformed into logarithmic scales. Tostabilize this low signal variability, the inventors add a constantvalue of 16 to the background corrected signal, a technique commonlyperformed on microarray data and the recommended data preprocessingmethod by Affymetrix and Illumina in the MicroArray Quality Control(MAQC) project (MAQC Consortium, 2006).

Normalization. The data were normalized with the VSN method (Huber etal., 2002). VSN is a global normalization process that stabilizes thevariance evenly across the entire range of expression. Differences inVSN-transformed expression between samples are denoted by “log 2Diff”and can be transformed to a generalized fold change via exponentiationbase 2. These values will exhibit a compression for small differences inexpression.

A total of 269 miRNAs (130 verified, 139 predicted) exhibitedsignificant differential expression between normal cervix (NCX) samplesand cancer-negative inguinal lymph nodes (LNneg), (t-test (LNneg vsNCX)<0.05 and log 2Diff (LNneg vs NCX)≦−1 (Table 4). Among the verifiedmiRNAs, the average expression levels of six miRNAs (hsa-miR-205, -368,-203, -495, -375, and -487b) were at least 100-fold less in LNnegsamples than in NCX samples; the average expression levels of ten miRNAs(hsa-miR-200a, -299-5p, -493-5p, -200b, -133b, -187, -133a, -200c, -411,and -127) were between 50- and 100-fold lower in LNneg samples; theaverage expression levels of 32 miRNAs were between 10- and 50-foldlower in LNneg samples; and the average expression levels of 23 miRNAswere between 5- and 10-fold lower in LNneg samples than in NCX samples.Among the predicted miRNAs, the average expression levels of threemiRNAs (hsa-asg-5021_st1, -13254_st1, and -9696_st1) were at least50-fold lower in LNneg samples than in NCX samples; the averageexpression levels of fourteen miRNAs were between 10- and 50-fold lowerin LNneg samples; and the average expression levels of 31 miRNAs werebetween 5- and 10-fold lower in LNneg samples than in NCX samples.

TABLE 4 MicroRNAs Differentially Expressed Between Normal Cervix TissueSamples (NCX) and Cancer-Negative Inguinal Lymph Node Samples (LNneg).NCX LNneg Micro RNA Probe ID 17 10 3 16 Mean SD % 1 2 hsa-miR-205hsa-miR-205_st1 12.8 8.63 12.99 12.18 11.67 2.05 100 3.61 2.06hsa-miR-368 hsa-miR-368_st2 11.2 11.6 11.37 11.34 11.39 0.15 100 4.354.22 hsa-miR-203 hsa-miR-203_st2 13.0 10.0 13.19 11.66 11.97 1.47 1004.94 5.50 hsa-miR-495 hsa-miR-495_st1 10.3 9.78 10.20 10.14 10.11 0.23100 3.22 2.72 hsa-miR-375 hsa-miR-375_st1 11.3 9.64 10.75 10.08 10.460.76 100 3.34 4.72 hsa-miR-487b hsa-miR-487b_st2 10.9 9.84 10.36 10.9110.52 0.53 100 4.55 4.06 hsa-asg-5021 hsa-asg-5021_st1 10.3 9.58 9.4710.23 9.91 0.44 100 4.04 3.24 hsa-miR-200a hsa-miR-200a_st2 11.2 11.410.47 10.21 10.84 0.60 100 4.99 5.73 hsa-miR-299-5p hsa-miR-299-5p_st29.8 9.74 9.53 9.62 9.69 0.15 100 4.04 3.47 hsa-miR-493-5phsa-miR-493-5p_st1 9.3 9.62 9.44 9.70 9.54 0.15 100 3.34 4.14hsa-asg-13254 hsa-asg-13254_st1 10.3 9.59 9.35 10.23 9.87 0.48 100 4.453.47 hsa-miR-200b hsa-miR-200b_st1 12.9 13.0 12.65 11.92 12.63 0.50 1006.68 7.72 hsa-miR-133b hsa-miR-133b_st2 8.6 11.2 10.91 10.49 10.33 1.14100 4.00 4.75 hsa-miR-187 hsa-miR-187_st1 8.9 9.31 8.58 8.08 8.72 0.53100 2.32 3.93 hsa-miR-133a hsa-miR-133a_st1 9.4 11.8 11.51 11.15 11.001.06 100 4.58 6.16 hsa-miR-200c hsa-miR-200c_st1 13.5 13.2 13.17 12.6313.15 0.39 100 7.89 8.26 hsa-miR-411 hsa-miR-411_st1 9.1 8.61 8.51 8.558.70 0.28 100 3.40 2.72 hsa-asg-9696 hsa-asg-9696_st1 10.5 11.1 10.7810.90 10.85 0.24 100 6.76 5.90 hsa-miR-127 hsa-miR-127_st1 11.6 11.210.89 11.77 11.40 0.39 100 6.07 5.95 hsa-miR-432 hsa-miR-432_st2 9.88.95 9.03 9.98 9.45 0.54 100 4.08 3.68 hsa-miR-424 hsa-miR-424_st1 9.710.2 9.93 9.47 9.83 0.31 100 5.04 5.70 hsa-asg-14153 hsa-asg-14153_st28.7 8.15 7.93 7.70 8.13 0.44 100 2.97 2.65 hsa-miR-455 hsa-miR-455_st18.5 8.65 10.11 8.97 9.06 0.73 100 2.65 5.19 hsa-miR-503 hsa-miR-503_st111.3 10.9 11.08 11.54 11.22 0.26 100 6.60 5.70 hsa-miR-503hsa-miR141_st1 11.2 10.8 11.09 11.36 11.16 0.21 100 6.90 6.05hsa-asg-14176 hsa-asg-14176_st1 9.0 9.48 9.71 8.86 9.26 0.40 100 4.765.47 hsa-miR-154 hsa-miR-154_st2 7.3 8.54 7.58 8.07 7.89 0.52 100 2.913.35 hsa-miR-381 hsa-miR-381_st2 7.4 8.10 7.78 7.83 7.78 0.28 100 3.812.19 hsa-miR-409-3p hsa-miR-409-3p_st1 8.5 7.37 7.47 8.23 7.91 0.58 1003.34 2.79 hsa-miR-199b hsa-miR-199b_st2 11.3 11.6 11.61 11.04 11.41 0.29100 6.16 7.61 hsa-miR-134 hsa-miR-134_st2 9.3 8.83 8.82 9.61 9.14 0.39100 4.79 4.51 hsa-miR-204 hsa-miR-204_st2 9.1 10.1 9.70 9.85 9.71 0.45100 5.15 6.57 hsa-miR-376a hsa-miR-376a_st2 8.9 7.92 8.29 8.12 8.32 0.46100 3.67 3.97 hsa-miR-376a hsa-miR-376a_st2 8.9 7.92 8.29 8.12 8.32 0.46100 3.67 3.97 hsa-miR-376a hsa-asg-14234_st1 8.3 7.12 7.22 7.17 7.470.59 100 3.45 4.14 hsa-asg-7637 hsa-asg-7637_st2 9.0 8.34 8.34 7.65 8.360.59 100 3.51 4.37 hsa-miR-189 hsa-miR-189_st1 8.3 8.65 8.24 8.47 8.430.17 100 3.81 4.70 hsa-miR-429 hsa-miR-429_st1 9.05 8.27 7.51 6.74 7.890.99 100 3.22 3.11 hsa-miR-450 hsa-miR-450_st2 6.92 7.82 7.82 8.19 7.690.54 100 3.10 3.58 hsa-miR-450 hsa-asg-14228_st2 5.3 7.20 7.32 7.54 6.861.00 100 2.84 3.78 hsa-miR-1 hsa-miR-1_st1 11.6 12.1 12.10 11.57 11.850.30 100 7.55 8.49 hsa-miR-494 hsa-miR-494_st1 10.6 9.49 9.36 10.04 9.870.57 100 5.33 5.47 hsa-miR-494 hsa-miR61_st2 9.4 8.67 8.54 9.22 8.970.44 100 5.51 5.36 hsa-miR-369-5p hsa-miR-369-5p_st2 7.2 7.19 6.82 7.987.32 0.48 100 2.71 3.11 hsa-miR-369-5p hsa-asg-1521_st1 7.0 6.46 5.797.31 6.65 0.68 75 3.22 3.24 hsa-miR-132 hsa-miR-132_st1 10.7 10.4 9.8910.44 10.39 0.37 100 7.27 6.66 hsa-asg-13097 hsa-asg-13097_st1 7.8 7.897.57 8.43 7.93 0.36 100 3.67 3.58 hsa-miR-379 hsa-miR-379_st1 11.0 10.09.98 10.50 10.38 0.47 100 6.01 6.25 hsa-miR-224 hsa-miR-224_st2 10.910.4 10.87 11.41 10.90 0.41 100 7.29 8.21 hsa-miR-224 hsa-asg-14247_st111.1 10.5 10.93 11.58 11.05 0.42 100 7.41 8.28 hsa-miR-196bhsa-miR-196b_st2 10.6 11.4 10.91 10.91 10.97 0.33 100 7.11 8.02hsa-cand206 hsa-cand206_st1 7.0 6.57 7.11 7.21 6.99 0.29 100 3.22 2.98hsa-miR-539 hsa-miR-539_st1 6.6 6.06 6.24 7.58 6.64 0.68 100 2.71 2.45hsa-miR-539 hsa-miR177_st1 5.6 6.07 6.26 6.79 6.18 0.49 50 2.71 3.68hsa-asg-5617 hsa-asg-5617_st1 7.3 7.04 7.16 7.70 7.32 0.29 100 2.97 3.35hsa-miR-382 hsa-miR-382_st1 9.6 9.68 10.26 10.69 10.07 0.50 100 6.246.05 hsa-miR202 hsa-miR202_st2 5.4 7.40 7.56 8.76 7.30 1.36 100 2.973.58 hsa-miR-9* hsa-miR-9-_st2 8.6 9.08 8.48 8.80 8.75 0.25 100 5.265.81 hsa-miR-542-5p hsa-miR-542-5p_st2 7.7 7.58 7.45 7.91 7.67 0.20 1004.08 2.98 hsa-asg-13404 hsa-asg-13404_st2 5.0 7.01 7.25 8.68 6.99 1.5075 3.72 2.72 hsa-asg-13166 hsa-asg-13166_st2 7.9 6.75 7.14 7.37 7.310.51 100 4.12 3.97 hsa-miR-218 hsa-miR-218_st2 11.3 11.3 10.99 11.1711.22 0.18 100 8.04 8.18 hsa-miR157 hsa-miR157_st2 11.5 11.5 11.50 11.8511.60 0.17 100 8.84 9.33 hsa-miR-9 hsa-miR-9_st2 6.4 8.35 6.98 6.84 7.150.83 100 3.77 3.78 hsa-miR-9 hsa-cand90_st2 5.3 7.12 5.55 5.99 6.00 0.7975 2.97 3.35 hsa-miR-329 hsa-miR-329_st1 7.1 6.85 6.47 6.83 6.82 0.27100 3.95 2.98 hsa-miR-329 hsa-asg-13589_st2 6.8 6.38 6.47 6.94 6.67 0.29100 2.97 2.72 hsa-miR-329 hsa-asg-13204_st2 7.1 6.49 6.59 7.24 6.85 0.37100 3.45 3.35 hsa-miR-452 hsa-miR-452_st1 8.9 8.51 8.24 8.82 8.62 0.31100 5.64 5.36 hsa-asg-562 hsa-asg-562_st1 12.9 12.8 12.86 13.10 12.950.11 100 10.29 10.53 hsa-miR-193b hsa-miR-193b_st1 10.9 11.1 10.30 10.7110.79 0.38 100 8.12 7.95 hsa-asg-10883 hsa-asg-10883_st1 9.1 10.3 9.288.88 9.43 0.66 100 6.15 7.10 hsa-asg-9687 hsa-asg-9687_st1 7.2 7.46 7.158.00 7.46 0.38 100 4.28 4.64 hsa-miR-485-5p hsa-miR-485-5p_st2 6.5 6.295.83 7.28 6.48 0.61 50 3.61 2.32 hsa-miR-605 hsa-miR-605_st1 6.6 6.125.76 5.79 6.07 0.40 100 2.25 2.85 hsa-asg-14134 hsa-asg-14134_st1 7.77.60 7.54 8.07 7.74 0.24 100 4.73 5.15 hsa-asg-8411 hsa-asg-8411_st26.52 7.56 7.04 7.70 7.20 0.54 100 3.56 4.57 hsa-asg-13297hsa-asg-13297_st1 6.77 3.76 6.35 8.26 6.28 1.87 75 2.91 2.45hsa-asg-14230 hsa-asg-14230_st1 6.6 6.66 6.94 6.93 6.80 0.16 100 3.564.06 hsa-asg-13284 hsa-asg-13284_st1 7.1 7.31 6.26 6.61 6.83 0.48 753.45 4.02 hsa-miR-433 hsa-miR-433_st2 7.0 6.42 5.98 7.53 6.74 0.68 753.72 2.85 hsa-asg-7472 hsa-asg-7472_st2 6.4 6.51 5.77 6.41 6.28 0.34 753.16 3.47 hsa-miR-214 hsa-miR-214_st1 11.4 12.0 11.74 12.29 11.89 0.36100 8.90 9.43 hsa-asg-10202 hsa-asg-10202_st2 6.5 6.63 5.35 6.05 6.140.58 50 2.58 4.67 hsa-miR-574 hsa-miR-574_st1 10.8 11.6 11.13 11.4211.27 0.36 100 8.40 9.16 hsa-miR-487a hsa-miR-487a_st2 6.2 6.06 6.336.27 6.24 0.12 100 3.45 3.47 hsa-miR-487a hsa-asg-6915_st1 7.2 6.17 6.936.63 6.73 0.44 100 4.12 4.44 hsa-asg-14172 hsa-asg-14172_st1 7.0 7.216.49 6.72 6.87 0.32 100 4.55 3.88 hsa-asg-10131 hsa-asg-10131_st2 5.86.45 6.29 5.79 6.10 0.32 100 2.71 3.24 hsa-miR-485-3p hsa-miR23_st2 7.26.48 6.45 7.07 6.82 0.41 100 3.61 4.06 hsa-miR-485-3p hsa-miR-485-3p_st26.8 6.16 6.35 6.96 6.58 0.38 100 3.91 4.37 hsa-miR-27b hsa-miR-27b_st113.6 13.8 13.84 13.50 13.71 0.16 100 11.25 11.47 hsa-miR-183hsa-miR-183_st1 8.9 8.36 9.07 7.70 8.53 0.64 100 6.58 5.76 hsa-miR-200a*hsa-miR-200a-_st2 6.8 6.50 6.10 5.28 6.18 0.68 75 3.16 3.47 hsa-miR-99bhsa-miR-99b_st1 12.2 12.3 12.15 12.48 12.32 0.14 100 10.18 10.43hsa-miR-99b hsa-asg-14196_st2 12.1 12.5 12.28 12.55 12.38 0.19 100 10.1510.74 hsa-miR-410 hsa-miR-410_st1 6.1 4.93 5.79 6.61 5.88 0.71 50 3.562.98 hsa-miR-497 hsa-miR-497_st1 9.1 9.43 10.28 8.72 9.40 0.66 100 6.757.90 hsa-miR-497 hsa-miR43_st1 10.1 10.1 10.89 9.53 10.19 0.56 100 7.898.53 hsa-miR154 hsa-miR154_st1 7.4 6.90 6.93 7.23 7.13 0.28 100 4.425.01 hsa-asg-1296 hsa-asg-1296_st1 6.7 6.30 6.07 5.74 6.21 0.41 50 3.223.11 hsa-miR-152 hsa-miR-152_st1 12.9 12.6 12.47 12.78 12.71 0.19 10010.29 10.97 hsa-miR-299-3p hsa-asg-10378_st1 6.1 5.13 6.53 6.05 5.960.59 75 2.78 3.24 hsa-miR-299-3p hsa-miR-299-3p_st2 5.8 5.47 5.76 5.895.74 0.19 25 3.67 2.98 hsa-asg-7465 hsa-asg-7465_st2 5.5 7.76 6.37 6.736.61 0.91 100 4.55 4.57 hsa-miR-182 hsa-miR-182_st1 10.9 10.1 10.35 9.4210.21 0.63 100 8.42 8.10 hsa-asg-9681 hsa-asg-9681_st1 6.5 6.75 6.646.90 6.71 0.16 100 4.12 4.22 hsa-asg-7471 hsa-asg-7471_st1 8.0 8.50 8.158.02 8.19 0.21 100 5.32 5.84 hsa-asg-11883 hsa-asg-11883_st1 8.4 8.038.10 8.30 8.21 0.18 100 4.97 5.73 hsa-asg-13205 hsa-asg-13205_st1 7.97.98 7.89 7.77 7.89 0.09 100 5.24 5.76 hsa-asg-14150 hsa-asg-14150_st17.5 7.47 7.89 7.47 7.60 0.20 75 5.45 6.15 hsa-cand247 hsa-cand247_st15.4 6.49 5.99 5.21 5.79 0.57 75 3.34 2.98 hsa-miR-654 hsa-asg-33_st1 7.26.04 5.89 7.03 6.54 0.67 50 3.81 3.11 hsa-miR-654 hsa-miR-654_st1 6.95.75 5.45 7.01 6.30 0.81 50 3.86 4.57 hsa-asg-2924 hsa-asg-2924_st1 5.46.70 6.31 5.81 6.06 0.57 25 3.77 3.68 hsa-asg-12227 hsa-asg-12227_st16.0 5.42 4.58 4.39 5.10 0.75 25 2.91 2.32 hsa-asg-10106hsa-asg-10106_st1 6.2 3.76 5.62 8.16 5.96 1.82 75 3.04 2.85 hsa-miR-130ahsa-cand144_st1 11.5 12.2 11.78 11.89 11.88 0.30 100 9.86 9.98hsa-miR-130a hsa-miR-130a_st1 11.4 12.1 11.79 11.67 11.77 0.31 100 9.959.59 hsa-miR-374 hsa-miR-374_st2 6.2 7.29 6.82 7.06 6.85 0.44 100 4.844.44 hsa-miR-199a hsa-miR-199a_st2 11.0 12.4 11.62 11.48 11.64 0.59 10010.04 10.07 hsa-miR-370 hsa-miR-370_st1 7.1 6.56 6.45 7.07 6.81 0.36 753.34 4.67 hsa-miR-582 hsa-miR-582_st1 4.2 6.95 6.98 6.73 6.24 1.31 753.67 4.91 hsa-miR-365 hsa-cand708_st1 6.3 6.57 5.94 7.17 6.52 0.51 1004.28 4.64 hsa-miR-365 hsa-miR-365_st1 7.0 7.28 6.79 8.10 7.31 0.56 1004.99 5.57 hsa-miR-365 hsa-asg-13156_st1 7.0 7.33 6.71 7.63 7.17 0.40 1004.35 5.84 hsa-miR-124a hsa-miR-124a_st1 7.8 7.21 5.72 5.91 6.68 1.04 1005.60 4.22 hsa-miR-124a hsa-cand79_st1 7.5 6.81 6.13 5.70 6.54 0.79 504.39 4.64 hsa-asg-12964 hsa-asg-12964_st2 4.7 5.65 5.37 6.10 5.46 0.5925 2.25 3.47 hsa-asg-279 hsa-asg-279_st2 7.1 8.28 7.61 7.09 7.53 0.55 505.61 5.96 hsa-asg-3822 hsa-asg-3822_st2 5.4 5.42 4.89 4.86 5.15 0.32 253.04 2.98 hsa-asg-11715 hsa-asg-11715_st2 5.8 4.95 5.03 5.05 5.22 0.4325 2.78 2.85 hsa-asg-13304 hsa-asg-13304_st2 7.9 8.47 8.05 7.57 8.020.37 100 6.04 6.50 hsa-miR-184 hsa-miR-184_st1 6.9 7.12 6.97 6.37 6.860.33 100 4.87 4.86 hsa-miR-27a hsa-miR-27a_st2 12.1 12.3 12.32 12.0712.23 0.14 100 10.49 10.77 hsa-asg-924 hsa-asg-924_st1 7.6 6.22 7.135.70 6.67 0.86 50 5.04 3.78 hsa-asg-3376 hsa-asg-3376_st1 6.6 6.18 6.766.01 6.40 0.36 100 4.31 3.78 hsa-miR-361 hsa-miR-361_st2 12.2 12.7 12.6512.74 12.60 0.24 100 10.69 11.37 hsa-asg-14233 hsa-asg-14233_st1 10.510.8 10.44 10.94 10.70 0.24 100 8.28 8.80 hsa-asg-8174 hsa-asg-8174_st15.7 6.00 5.76 5.15 5.66 0.36 75 2.91 3.35 hsa-asg-2837 hsa-asg-2837_st23.7 5.96 5.85 5.31 5.21 1.02 50 3.72 2.98 hsa-asg-10674hsa-asg-10674_st1 7.3 7.61 6.93 7.35 7.32 0.28 100 4.76 4.75hsa-asg-14146 hsa-asg-14146_st1 7.2 7.26 7.11 6.22 6.97 0.51 100 4.735.32 hsa-miR-376b hsa-miR-376b_st1 5.9 3.89 4.80 5.18 4.95 0.85 50 2.712.19 hsa-miR-211 hsa-miR-211_st1 7.1 5.35 3.47 6.15 5.52 1.54 50 3.723.47 hsa-miR-154* hsa-miR-154-_st2 7.0 6.29 6.53 6.52 6.60 0.32 100 4.675.36 hsa-asg-259 hsa-asg-259_st2 5.5 5.76 5.99 5.28 5.64 0.31 25 3.863.24 hsa-asg-1596 hsa-asg-1596_st2 6.2 6.04 5.95 5.50 5.93 0.31 25 4.243.24 hsa-asg-13646 hsa-asg-13646_st1 9.2 8.59 8.40 8.70 8.74 0.38 1006.92 7.78 hsa-asg-14175 hsa-asg-14175_st1 6.5 6.87 6.62 7.38 6.85 0.39100 4.49 5.70 hsa-asg-4439 hsa-asg-4439_st2 7.5 5.07 5.03 5.15 5.70 1.2425 3.67 3.58 hsa-miR-99a hsa-miR-99a_st2 12.9 13.2 13.21 13.09 13.140.13 100 11.03 12.19 hsa-miR-99a hsa-cand160_st2 12.9 13.0 13.04 12.9412.98 0.08 100 11.36 12.46 hsa-miR-499 hsa-miR-499_st1 4.6 6.82 5.666.36 5.87 0.95 50 3.22 5.15 hsa-miR-499 hsa-miR12_st1 5.5 7.24 5.31 5.996.02 0.86 75 3.77 4.86 hsa-asg-3145 hsa-asg-3145_st1 6.2 6.83 6.24 7.316.67 0.50 75 4.20 5.06 hsa-miR-504 hsa-miR-504_st1 5.5 6.43 5.25 5.845.77 0.50 25 3.86 3.47 hsa-miR-489 hsa-miR-489_st2 6.9 6.51 7.21 6.926.90 0.29 100 5.45 5.24 hsa-asg-12139 hsa-asg-12139_st2 5.7 4.98 4.685.01 5.11 0.46 25 2.97 3.35 hsa-miR-149 hsa-miR-149_st2 6.4 6.24 5.956.74 6.34 0.33 75 3.45 5.11 hsa-asg-9198 hsa-asg-9198_st1 5.2 3.23 5.513.84 4.45 1.10 25 2.91 2.72 hsa-asg-9980 hsa-asg-9980_st1 6.4 5.33 5.885.31 5.73 0.52 50 2.91 4.14 hsa-miR-409-5p hsa-miR-409-5p_st1 8.0 7.397.37 8.08 7.72 0.39 100 5.81 5.28 hsa-asg-13705 hsa-asg-13705_st1 5.65.38 5.03 4.94 5.24 0.31 25 3.10 2.72 hsa-asg-13270 hsa-asg-13270_st25.4 6.68 5.64 4.94 5.67 0.74 50 4.00 4.14 hsa-asg-2027 hsa-asg-2027_st14.4 5.58 5.79 5.01 5.20 0.62 25 3.67 2.19 hsa-asg-360 hsa-asg-360_st25.7 6.12 6.26 5.12 5.80 0.51 25 4.20 3.97 hsa-miR-148b hsa-miR-148b_st29.7 10.2 10.21 9.76 9.99 0.28 100 9.00 9.14 hsa-asg-13011hsa-asg-13011_st2 5.9 6.21 5.39 5.77 5.84 0.35 75 4.49 3.78 hsa-miR-576hsa-miR-576_st2 6.2 3.61 5.47 5.18 5.13 1.11 25 2.84 2.98 hsa-asg-11361hsa-asg-11361_st2 5.1 4.83 5.51 5.05 5.15 0.29 50 3.34 2.98 hsa-miR-148ahsa-miR-148a_st1 11.9 11.2 11.80 11.55 11.63 0.32 100 10.21 10.99hsa-cand207 hsa-cand207_st1 5.5 4.75 5.72 5.97 5.50 0.53 75 4.00 3.24hsa-miR-34b hsa-miR-34b_st2 6.3 6.86 6.68 5.95 6.45 0.40 100 5.04 4.44hsa-asg-13258 hsa-asg-13258_st2 9.8 9.28 8.82 9.26 9.30 0.42 100 7.648.26 hsa-asg-2301 hsa-asg-2301_st2 7.3 7.99 7.50 8.25 7.78 0.41 100 6.076.27 hsa-miR-421 hsa-miR-421-3p_st1 9.2 9.68 9.72 9.86 9.63 0.27 1007.97 8.53 hsa-miR-421 hsa-asg-5033_st2 8.2 9.02 8.86 9.10 8.81 0.38 1007.14 7.53 hsa-asg-8344 hsa-asg-8344_st2 5.1 5.82 5.85 5.37 5.55 0.34 503.22 4.57 hsa-miR-10a hsa-miR-10a_st1 12.3 12.2 12.00 12.08 12.16 0.17100 10.73 11.47 hsa-asg-2365 hsa-asg-2365_st1 6.5 7.26 7.04 6.19 6.760.48 100 5.35 5.50 hsa-asg-10247 hsa-asg-10247_st1 5.4 5.58 5.18 4.555.18 0.45 25 3.10 3.11 hsa-asg-10626 hsa-asg-10626_st2 6.4 5.66 5.885.70 5.92 0.36 100 4.58 4.51 hsa-asg-13997 hsa-asg-13997_st2 5.5 3.515.99 5.45 5.11 1.10 75 2.71 3.11 hsa-miR-493-3p hsa-miR-493-3p_st1 6.65.63 6.44 5.98 6.18 0.46 50 4.79 4.14 hsa-miR-28 hsa-miR-28_st1 11.511.4 11.26 11.63 11.47 0.15 100 10.20 10.44 hsa-miR-340 hsa-miR-340_st15.3 6.14 6.57 6.50 6.14 0.56 50 4.49 5.36 hsa-miR-135b hsa-cand78_st14.8 6.08 4.06 5.37 5.09 0.85 25 4.04 2.98 hsa-asg-10830hsa-asg-10830_st2 5.1 5.44 5.08 4.73 5.11 0.29 25 3.40 3.97 hsa-miR-585hsa-miR-585_st1 5.4 5.55 4.36 5.50 5.21 0.57 25 3.16 3.24 hsa-asg-14138hsa-asg-14138_st2 6.5 6.84 5.77 6.77 6.48 0.49 75 5.51 4.96hsa-asg-11199 hsa-asg-11199_st2 12.9 12.6 12.19 12.60 12.60 0.33 10011.55 11.67 hsa-miR-151 hsa-miR-151_st2 10.8 10.8 10.45 10.69 10.70 0.18100 9.51 9.56 hsa-asg-8428 hsa-asg-8428_st2 5.8 4.29 5.22 4.50 4.96 0.7125 3.22 3.47 hsa-asg-4219 hsa-asg-4219_st1 5.1 5.37 4.97 4.69 5.04 0.2825 3.04 3.47 hsa-miR-324-3p hsa-miR-324-3p_st1 8.3 9.23 8.69 8.94 8.800.38 25 7.28 7.58 hsa-miR-422a hsa-miR-422a_st1 9.1 9.56 9.65 9.46 9.440.24 100 7.31 8.59 hsa-asg-2967 hsa-asg-2967_st2 5.1 5.76 5.44 5.15 5.380.28 25 3.56 4.06 hsa-miR-34a hsa-miR-34a_st1 9.4 9.90 9.78 9.51 9.660.21 100 8.60 9.04 hsa-miR-100 hsa-miR-100_st2 13.0 13.2 13.20 13.1313.17 0.07 100 11.57 12.33 hsa-miR-100 hsa-cand375_st1 12.8 12.9 12.8712.79 12.85 0.07 100 11.65 12.30 hsa-miR-30e-3p hsa-miR-30e-3p_st1 8.48.84 8.96 8.43 8.66 0.28 100 7.46 8.19 hsa-asg-8878 hsa-asg-8878_st210.3 10.7 9.94 11.14 10.54 0.51 100 9.33 9.97 hsa-miR-422bhsa-miR-422b_st2 10.6 11.2 11.15 10.91 10.97 0.27 100 9.09 10.11hsa-miR-542-3p hsa-miR-542-3p_st1 7.8 7.24 7.82 7.32 7.55 0.31 100 6.035.90 hsa-asg-10336 hsa-asg-10336_st1 5.6 5.50 5.13 4.97 5.31 0.32 253.95 4.37 hsa-miR-561 hsa-miR-561_st1 5.0 3.61 6.31 4.17 4.78 1.18 253.10 3.24 hsa-miR-141 hsa-miR-141_st1 7.6 5.96 5.35 5.68 6.16 1.03 754.64 5.28 hsa-asg-3967 hsa-asg-3967_st2 8.0 8.37 8.15 8.30 8.21 0.16 1006.90 7.18 hsa-asg-280 hsa-asg-280_st1 5.2 4.88 4.97 4.34 4.86 0.38 253.22 3.24 hsa-asg-548 hsa-asg-548_st1 5.0 5.58 4.15 4.86 4.91 0.59 253.45 3.88 hsa-miR-628 hsa-miR-628_st2 8.1 7.99 8.05 8.80 8.25 0.38 1006.87 7.26 hsa-miR-452* hsa-miR-452-_st1 5.8 4.86 3.86 4.17 4.69 0.89 253.10 2.98 hsa-miR-193a hsa-miR-193a_st1 5.2 5.98 5.79 6.17 5.80 0.39 1004.39 3.97 hsa-cand26 hsa-cand26_st2 6.8 6.74 6.37 7.81 6.93 0.62 1005.28 5.92 hsa-cand720 hsa-cand720_st1 6.8 6.28 6.81 7.01 6.74 0.32 1004.79 5.83 hsa-asg-5715 hsa-asg-5715_st2 6.2 6.53 6.08 6.23 6.28 0.19 754.73 5.79 hsa-miR-331 hsa-asg-14205_st1 7.4 7.94 7.92 8.24 7.90 0.31 1006.37 7.04 hsa-miR-331 hsa-miR-331_st2 8.7 9.16 9.04 9.45 9.10 0.29 1007.95 8.77 hsa-asg-10391 hsa-asg-10391_st2 6.7 6.59 6.85 5.12 6.32 0.8175 4.73 4.94 hsa-asg-11380 hsa-asg-11380_st2 5.1 3.80 5.88 4.11 4.740.96 25 2.97 3.47 hsa-miR-324-5p hsa-miR-324-5p_st1 8.8 9.61 9.11 9.189.18 0.33 100 7.44 8.03 hsa-asg-6758 hsa-asg-6758_st1 5.2 4.91 4.86 4.284.82 0.39 25 4.00 2.98 hsa-asg-8380 hsa-asg-8380_st2 4.8 5.82 6.33 4.975.49 0.70 25 3.86 4.91 hsa-cand330 hsa-cand330_st2 5.8 4.93 4.68 5.685.29 0.57 50 4.35 4.37 hsa-asg-13279 hsa-asg-13279_st1 7.6 7.85 7.718.11 7.84 0.20 100 6.65 7.16 hsa-asg-11181 hsa-asg-11181_st1 5.8 6.976.43 6.86 6.53 0.50 50 5.04 5.41 hsa-miR-338 hsa-miR-338_st1 4.9 6.174.74 6.03 5.48 0.72 75 4.04 4.30 hsa-miR-186 hsa-miR-186_st1 7.3 7.887.63 7.83 7.67 0.26 100 6.60 7.17 hsa-asg-3811 hsa-asg-3811_st1 11.312.0 11.88 11.92 11.79 0.34 100 10.68 11.14 hsa-asg-13406hsa-asg-13406_st2 4.6 5.33 5.59 4.28 4.96 0.60 25 3.40 3.88hsa-asg-12465 hsa-asg-12465_st1 5.5 5.54 5.31 5.74 5.53 0.18 25 4.554.57 hsa-asg-3902 hsa-asg-3902_st1 5.3 4.86 4.80 4.94 4.99 0.26 25 3.284.06 hsa-asg-11877 hsa-asg-11877_st1 6.8 4.70 4.83 5.82 5.54 0.99 254.20 3.78 hsa-miR-143 hsa-miR-143_st2 13.2 13.1 13.01 13.04 13.11 0.10100 12.27 12.46 hsa-asg-3998 hsa-asg-3998_st1 8.5 9.01 9.20 8.71 8.870.29 100 7.22 8.34 hsa-asg-10278 hsa-asg-10278_st2 4.3 5.33 5.33 5.325.09 0.47 75 3.91 4.10 hsa-asg-4122 hsa-asg-4122_st2 5.7 7.21 6.90 5.556.35 0.82 100 5.08 5.50 hsa-asg-10113 hsa-asg-10113_st1 6.1 4.98 5.394.90 5.35 0.56 25 3.34 4.06 hsa-miR-128b hsa-miR-128b_st1 10.7 10.510.60 10.95 10.71 0.20 100 10.02 10.11 hsa-asg-9723 hsa-asg-9723_st2 4.93.29 5.66 4.04 4.49 1.04 50 2.84 3.24 hsa-miR-128a hsa-miR-128a_st2 10.210.0 10.23 10.48 10.24 0.18 100 9.46 9.69 hsa-miR-31 hsa-miR-31_st2 12.711.7 12.03 11.12 11.92 0.69 100 11.34 10.17 hsa-asg-14048hsa-asg-14048_st1 5.7 5.44 5.31 5.74 5.56 0.23 50 4.58 4.06hsa-asg-11906 hsa-asg-11906_st1 5.0 6.42 5.33 5.79 5.64 0.60 50 3.814.64 hsa-asg-9473 hsa-asg-9473_st2 7.0 6.83 7.98 6.78 7.15 0.56 100 5.176.63 hsa-miR-30d hsa-miR-30d_st1 12.9 13.2 13.06 13.01 13.05 0.11 10012.27 12.40 hsa-asg-12083 hsa-asg-12083_st1 6.3 6.28 5.51 5.93 6.03 0.4075 4.87 4.51 hsa-miR-30a-5p hsa-miR-30a-5p_st2 12.2 12.5 12.35 12.5112.42 0.14 100 11.56 11.94 hsa-miR-425-3p hsa-miR-425_st2 6.9 7.70 6.687.06 7.11 0.43 75 5.98 6.96 hsa-asg-12346 hsa-asg-12346_st2 7.3 8.237.88 8.50 7.98 0.51 75 6.78 7.13 hsa-miR-98 hsa-miR-98_st1 11.5 11.811.49 11.71 11.64 0.14 100 10.51 11.17 hsa-asg-5304 hsa-asg-5304_st110.4 10.6 10.71 10.87 10.65 0.19 100 9.51 10.56 hsa-asg-11285hsa-asg-11285_st2 5.3 5.23 5.62 5.31 5.38 0.17 50 4.49 4.06 hsa-asg-153hsa-asg-153_st2 5.5 4.61 4.55 4.17 4.72 0.58 25 2.32 4.06 hsa-miR-212hsa-miR-212_st2 7.4 6.93 5.86 6.76 6.74 0.64 75 6.14 5.50 hsa-asg-13135hsa-asg-13135_st1 6.6 6.59 6.22 7.81 6.83 0.69 100 5.83 6.02hsa-asg-13308 hsa-asg-13308_st2 10.3 10.5 10.34 10.79 10.50 0.21 1009.47 10.39 hsa-asg-4542 hsa-asg-4542_st2 5.8 5.91 5.22 5.81 5.69 0.32 754.70 4.81 hsa-asg-8266 hsa-asg-8266_st2 5.9 5.60 5.51 4.69 5.45 0.54 504.31 4.44 hsa-asg-9848 hsa-asg-9848_st1 5.3 5.37 5.03 6.60 5.59 0.69 253.77 4.64 hsa-asg-5873 hsa-asg-5873_st2 5.5 6.72 6.85 6.28 6.36 0.58 1004.84 5.95 hsa-asg-7803 hsa-asg-7803_st2 4.8 5.88 4.15 5.70 5.15 0.80 504.00 4.30 hsa-asg-3038 hsa-asg-3038_st2 6.3 6.68 6.08 5.91 6.25 0.33 755.19 4.96 hsa-miR-320 hsa-miR-320_st1 12.0 12.3 12.27 12.42 12.28 0.17100 11.35 11.67 hsa-miR-598 hsa-miR-598_st2 6.7 7.29 6.35 7.19 6.90 0.43100 5.76 6.55 hsa-miR-30e-5p hsa-miR-30e-5p_st2 10.0 10.7 10.29 10.3410.35 0.26 100 9.26 10.26 hsa-asg-13661 hsa-asg-13661_st1 7.3 6.05 5.516.48 6.34 0.77 50 4.94 5.55 hsa-let-7e hsa-let-7e_st1 13.0 12.9 12.8313.24 13.03 0.17 100 12.02 12.57 hsa-asg-1399 hsa-asg-1399_st1 5.6 5.095.08 5.79 5.39 0.36 50 4.92 4.06 hsa-asg-12403 hsa-asg-12403_st2 5.66.27 5.08 6.39 5.84 0.61 75 5.04 5.19 hsa-asg-1782 hsa-asg-1782_st2 4.54.55 3.86 4.39 4.34 0.32 25 2.71 3.88 hsa-asg-8476 hsa-asg-8476_st1 7.47.85 7.61 7.75 7.65 0.19 75 5.64 7.01 hsa-miR-197 hsa-miR-197_st1 10.29.70 9.81 10.48 10.07 0.37 100 9.71 9.36 hsa-miR-24 hsa-miR-24_st1 13.613.6 13.55 13.52 13.60 0.07 100 12.87 13.03 hsa-asg-5973hsa-asg-5973_st1 5.7 6.17 6.31 6.19 6.11 0.23 100 5.68 4.91 hsa-asg-5740hsa-asg-5740_st2 5.7 5.35 6.00 5.45 5.64 0.30 25 4.31 5.15 hsa-miR-376a*hsa-miR-376a-_st2 5.6 5.42 5.69 5.55 5.59 0.13 50 5.04 4.81 hsa-asg-3614hsa-asg-3614_st1 6.2 4.86 4.92 5.05 5.27 0.67 25 4.12 4.26 hsa-miR-641hsa-miR-641_st1 5.6 3.76 4.32 4.17 4.47 0.81 25 3.22 3.35 hsa-asg-2596hsa-asg-2596_st2 4.4 5.47 5.44 6.03 5.35 0.64 25 4.45 4.86 hsa-asg-2992hsa-asg-2992_st1 5.2 6.64 6.10 6.01 6.00 0.56 25 4.61 5.04hsa-miR-30a-3p hsa-miR-30a-3p_st1 8.9 8.63 8.91 9.56 9.00 0.39 100 8.198.70 hsa-asg-3604 hsa-asg-3604_st1 6.1 6.57 6.41 6.05 6.31 0.23 100 5.615.95 hsa-miR-454-3p hsa-asg-11541_st2 8.7 8.94 8.30 8.87 8.71 0.29 1008.05 8.30 hsa-asg-8737 hsa-asg-8737_st1 8.9 8.79 8.58 9.11 8.86 0.23 757.74 7.71 hsa-asg-8354 hsa-asg-8354_st2 4.7 4.70 4.06 5.81 4.82 0.73 253.72 3.97 hsa-asg-12998 hsa-asg-12998_st1 4.4 4.88 5.91 4.94 5.03 0.6325 3.77 4.14 hsa-asg-9815 hsa-asg-9815_st2 6.2 6.66 6.50 6.32 6.43 0.1975 5.48 5.73 hsa-asg-5053 hsa-asg-5053_st1 6.5 5.94 6.73 6.61 6.46 0.35100 5.76 5.57 hsa-asg-13905 hsa-asg-13905_st1 5.4 5.37 4.74 3.91 4.870.72 25 3.72 4.44 hsa-asg-11397 hsa-asg-11397_st1 5.1 4.83 3.76 4.904.66 0.62 25 3.34 3.88 hsa-let-7b hsa-let-7b_st2 14.3 14.4 14.50 14.2414.37 0.12 100 13.9 14.0 hsa-miR-510 hsa-miR-510_st2 5.5 5.44 5.69 5.375.51 0.14 50 4.58 4.91 hsa-asg-14015 hsa-asg-14015_st1 6.3 5.00 6.316.01 5.91 0.62 25 4.79 5.34 LNneg vs NCX LNneg Log2 Fold Micro RNA 3 4Mean SD % ttest Diff change hsa-miR-205 2.65 3.57 2.98 0.75 0 2.11E−04−8.69 413.8 hsa-miR-368 3.32 3.48 3.84 0.52 0 1.40E−07 −7.55 187.2hsa-miR-203 3.59 4.15 4.55 0.84 0 1.25E−04 −7.42 171.5 hsa-miR-495 3.122.94 3.00 0.22 0 8.71E−09 −7.11 138.2 hsa-miR-375 4.05 2.47 3.65 0.97 03.19E−05 −6.81 112.3 hsa-miR-487b 2.71 3.92 3.81 0.78 0 7.75E−06 −6.71104.8 hsa-asg-5021 3.12 2.82 3.31 0.52 0 1.24E−06 −6.60 97.3hsa-miR-200a 3.83 3.48 4.51 1.04 25 4.23E−05 −6.33 80.6 hsa-miR-299-5p2.71 3.38 3.40 0.55 0 5.39E−07 −6.29 78.3 hsa-miR-493-5p 2.38 3.27 3.280.72 0 2.61E−06 −6.25 76.2 hsa-asg-13254 3.76 3.11 3.70 0.57 0 3.03E−06−6.17 72.2 hsa-miR-200b 5.92 6.17 6.62 0.80 100 1.41E−05 −6.01 64.3hsa-miR-133b 4.96 3.57 4.32 0.65 0 9.41E−05 −6.01 64.2 hsa-miR-187 2.762.01 2.75 0.84 0 1.98E−05 −5.97 62.7 hsa-miR-133a 5.51 3.96 5.05 0.97 01.69E−04 −5.94 61.4 hsa-miR-200c 7.04 6.42 7.40 0.83 100 1.59E−05 −5.7553.7 hsa-miR-411 2.92 2.88 2.98 0.29 0 1.26E−07 −5.72 52.5 hsa-asg-96964.37 3.80 5.21 1.36 25 1.82E−04 −5.65 50.1 hsa-miR-127 5.48 5.52 5.760.30 25 4.67E−07 −5.65 50.0 hsa-miR-432 2.81 4.67 3.81 0.78 0 2.13E−05−5.64 49.9 hsa-miR-424 3.91 2.71 4.34 1.31 25 1.85E−04 −5.49 45.1hsa-asg-14153 2.71 2.59 2.73 0.17 0 4.57E−07 −5.40 42.2 hsa-miR-455 3.503.43 3.69 1.07 0 1.70E−04 −5.36 41.1 hsa-miR-503 5.42 5.75 5.87 0.51 251.49E−06 −5.36 41.0 hsa-miR-503 6.06 6.23 6.31 0.40 75 7.07E−07 −4.8528.8 hsa-asg-14176 4.22 2.59 4.26 1.22 0 2.40E−04 −5.00 32.1 hsa-miR-1542.49 3.22 2.99 0.38 0 5.09E−06 −4.90 29.9 hsa-miR-381 2.92 2.77 2.920.67 0 1.10E−05 −4.86 29.0 hsa-miR-409-3p 3.12 3.16 3.10 0.23 0 4.59E−06−4.80 27.9 hsa-miR-199b 6.54 6.50 6.71 0.63 100 9.91E−06 −4.71 26.1hsa-miR-134 4.42 4.22 4.49 0.23 0 8.49E−07 −4.66 25.3 hsa-miR-204 5.133.43 5.07 1.29 25 4.92E−04 −4.64 24.9 hsa-miR-376a 3.83 3.27 3.69 0.30 02.76E−06 −4.64 24.9 hsa-miR-376a 3.83 3.27 3.69 0.30 0 2.76E−06 −4.6424.9 hsa-miR-376a 2.92 3.38 3.47 0.50 0 4.93E−05 −3.99 15.9 hsa-asg-76374.09 3.05 3.75 0.59 0 3.28E−05 −4.60 24.3 hsa-miR-189 3.17 3.67 3.840.63 0 8.41E−06 −4.59 24.1 hsa-miR-429 3.59 3.38 3.33 0.21 0 1.05E−04−4.57 23.7 hsa-miR-450 3.02 3.27 3.24 0.25 0 5.73E−06 −4.45 21.8hsa-miR-450 3.41 3.76 3.45 0.44 0 7.77E−04 −3.41 10.6 hsa-miR-1 8.015.75 7.45 1.20 100 3.80E−04 −4.41 21.2 hsa-miR-494 5.10 6.09 5.50 0.4350 1.78E−05 −4.38 20.8 hsa-miR-494 5.74 6.05 5.67 0.30 75 1.64E−05 −3.309.9 hsa-miR-369-5p 2.60 3.57 3.00 0.44 0 1.18E−05 −4.32 19.9hsa-miR-369-5p 2.49 3.16 3.03 0.36 0 7.96E−05 −3.62 12.3 hsa-miR-1326.00 4.42 6.09 1.23 25 5.37E−04 −4.30 19.7 hsa-asg-13097 3.76 3.84 3.710.11 0 5.42E−07 −4.22 18.7 hsa-miR-379 6.26 6.20 6.18 0.11 75 2.28E−06−4.20 18.4 hsa-miR-224 5.67 5.82 6.75 1.22 50 6.69E−04 −4.15 17.7hsa-miR-224 5.81 6.49 7.00 1.08 100 4.19E−04 −4.06 16.7 hsa-miR-196b6.75 5.43 6.83 1.07 100 3.20E−04 −4.14 17.6 hsa-cand206 2.60 2.59 2.850.31 0 1.12E−06 −4.14 17.6 hsa-miR-539 2.38 2.47 2.51 0.14 0 2.13E−05−4.13 17.6 hsa-miR-539 3.22 3.05 3.17 0.40 0 7.97E−05 −3.01 8.1hsa-asg-5617 2.49 3.92 3.19 0.61 0 1.74E−05 −4.13 17.6 hsa-miR-382 5.086.41 5.94 0.59 25 4.17E−05 −4.12 17.4 hsa-miR202 3.22 3.05 3.21 0.27 01.05E−03 −4.09 17.0 hsa-miR-9* 4.37 4.00 4.86 0.83 25 1.03E−04 −3.8914.9 hsa-miR-542-5p 4.53 3.62 3.80 0.66 0 3.02E−05 −3.86 14.6hsa-asg-13404 2.97 3.27 3.17 0.43 0 2.71E−03 −3.82 14.1 hsa-asg-131662.92 3.05 3.52 0.62 0 7.79E−05 −3.79 13.8 hsa-miR-218 7.05 6.47 7.430.82 100 1.02E−04 −3.78 13.8 hsa-miR157 7.29 6.15 7.90 1.46 75 2.35E−03−3.70 13.0 hsa-miR-9 2.60 3.92 3.52 0.62 0 4.21E−04 −3.63 12.4 hsa-miR-93.12 3.16 3.15 0.16 0 4.15E−04 −2.85 7.2 hsa-miR-329 3.41 2.47 3.21 0.630 4.23E−05 −3.61 12.2 hsa-miR-329 3.83 3.05 3.15 0.48 0 1.51E−05 −3.5311.5 hsa-miR-329 3.02 4.00 3.46 0.41 0 1.74E−05 −3.40 10.5 hsa-miR-4523.67 5.36 5.01 0.90 0 2.72E−04 −3.61 12.2 hsa-asg-562 8.97 7.63 9.351.34 100 1.72E−03 −3.59 12.1 hsa-miR-193b 6.26 6.66 7.25 0.93 504.01E−04 −3.54 11.6 hsa-asg-10883 4.79 5.55 5.90 0.97 25 9.64E−04 −3.5311.5 hsa-asg-9687 3.76 3.16 3.96 0.64 0 8.41E−05 −3.50 11.3hsa-miR-485-5p 2.87 3.27 3.02 0.56 0 1.54E−04 −3.46 11.0 hsa-miR-6053.02 2.53 2.66 0.34 0 1.30E−05 −3.40 10.6 hsa-asg-14134 3.41 4.15 4.360.75 0 1.41E−04 −3.38 10.4 hsa-asg-8411 3.76 3.67 3.89 0.46 0 8.39E−05−3.31 9.9 hsa-asg-13297 3.22 3.32 2.98 0.39 0 1.36E−02 −3.31 9.9hsa-asg-14230 3.50 3.05 3.54 0.41 0 6.08E−06 −3.25 9.5 hsa-asg-132843.55 3.38 3.60 0.29 0 2.50E−05 −3.23 9.4 hsa-miR-433 3.80 3.76 3.53 0.450 2.28E−04 −3.21 9.3 hsa-asg-7472 2.12 3.67 3.10 0.69 0 1.69E−04 −3.189.0 hsa-miR-214 8.56 7.98 8.72 0.61 100 1.07E−04 −3.17 9.0 hsa-asg-102022.12 2.82 3.05 1.12 0 2.69E−03 −3.09 8.5 hsa-miR-574 8.02 7.16 8.18 0.83100 4.87E−04 −3.08 8.5 hsa-miR-487a 3.50 2.24 3.17 0.62 0 6.77E−05 −3.078.4 hsa-miR-487a 4.53 4.00 4.27 0.25 0 6.99E−05 −2.46 5.5 hsa-asg-141723.02 3.76 3.80 0.63 0 1.28E−04 −3.07 8.4 hsa-asg-10131 3.50 2.71 3.040.40 0 2.06E−05 −3.06 8.3 hsa-miR-485-3p 3.59 3.80 3.77 0.22 0 1.18E−05−3.05 8.3 hsa-miR-485-3p 3.67 4.12 4.02 0.30 0 4.21E−05 −2.56 5.9hsa-miR-27b 10.47 9.46 10.6 0.91 100 5.85E−04 −3.04 8.2 hsa-miR-183 4.315.77 5.60 0.95 25 2.16E−03 −2.92 7.6 hsa-miR-200a* 3.67 2.77 3.27 0.40 03.15E−04 −2.92 7.6 hsa-miR-99b 8.61 8.42 9.41 1.04 50 1.46E−03 −2.91 7.5hsa-miR-99b 8.83 8.43 9.54 1.09 75 2.09E−03 −2.84 7.2 hsa-miR-410 2.173.16 2.97 0.58 0 7.50E−04 −2.90 7.5 hsa-miR-497 5.27 6.05 6.49 1.12 254.20E−03 −2.90 7.5 hsa-miR-497 6.29 7.02 7.43 0.98 50 2.77E−03 −2.75 6.7hsa-miR154 3.67 3.84 4.24 0.61 0 1.30E−04 −2.90 7.4 hsa-asg-1296 4.122.94 3.35 0.53 0 1.37E−04 −2.86 7.3 hsa-miR-152 9.52 8.66 9.86 1.00 1001.37E−03 −2.85 7.2 hsa-miR-299-3p 2.49 3.96 3.12 0.64 0 6.24E−04 −2.847.2 hsa-miR-299-3p 3.22 4.00 3.47 0.45 0 9.09E−05 −2.27 4.8 hsa-asg-74653.02 2.94 3.77 0.91 0 4.57E−03 −2.84 7.1 hsa-miR-182 5.76 7.23 7.38 1.19100 5.61E−03 −2.83 7.1 hsa-asg-9681 3.83 3.48 3.91 0.33 0 5.21E−06 −2.796.9 hsa-asg-7471 5.42 5.05 5.41 0.33 0 7.89E−06 −2.78 6.9 hsa-asg-118835.91 5.15 5.44 0.45 0 2.73E−05 −2.77 6.8 hsa-asg-13205 4.92 4.70 5.150.46 0 2.36E−05 −2.73 6.6 hsa-asg-14150 3.98 3.92 4.88 1.10 0 2.83E−03−2.73 6.6 hsa-cand247 3.02 2.94 3.07 0.18 0 9.58E−05 −2.72 6.6hsa-miR-654 4.02 4.35 3.82 0.52 0 7.01E−04 −2.72 6.6 hsa-miR-654 3.833.48 3.94 0.46 0 2.32E−03 −2.36 5.1 hsa-asg-2924 3.59 2.35 3.35 0.67 08.16E−04 −2.71 6.5 hsa-asg-12227 2.12 2.35 2.43 0.34 0 6.31E−04 −2.676.4 hsa-asg-10106 3.91 3.43 3.31 0.47 0 3.03E−02 −2.65 6.3 hsa-miR-130a8.95 8.16 9.24 0.85 100 1.10E−03 −2.64 6.3 hsa-miR-130a 8.76 8.21 9.130.79 100 7.73E−04 −2.64 6.2 hsa-miR-374 3.91 3.84 4.26 0.47 0 2.03E−04−2.60 6.0 hsa-miR-199a 8.37 7.75 9.06 1.18 100 7.81E−03 −2.58 6.0hsa-miR-370 4.61 4.42 4.26 0.62 0 3.79E−04 −2.56 5.9 hsa-miR-582 2.813.38 3.69 0.89 0 1.82E−02 −2.54 5.8 hsa-miR-365 4.25 2.82 4.00 0.80 01.81E−03 −2.52 5.7 hsa-miR-365 4.94 4.48 5.00 0.45 0 6.69E−04 −2.32 5.0hsa-miR-365 5.45 4.08 4.93 0.85 25 3.08E−03 −2.24 4.7 hsa-miR-124a 3.023.80 4.16 1.08 0 1.50E−02 −2.52 5.7 hsa-miR-124a 3.46 4.15 4.16 0.51 02.30E−03 −2.38 5.2 hsa-asg-12964 2.54 3.48 2.94 0.63 0 1.10E−03 −2.525.7 hsa-asg-279 4.42 4.15 5.04 0.88 0 3.07E−03 −2.49 5.6 hsa-asg-38222.12 2.59 2.68 0.42 0 8.87E−05 −2.47 5.5 hsa-asg-11715 3.22 2.18 2.760.43 0 1.92E−04 −2.46 5.5 hsa-asg-13304 5.04 4.65 5.56 0.86 25 1.91E−03−2.46 5.5 hsa-miR-184 3.22 4.67 4.41 0.79 0 1.26E−03 −2.45 5.5hsa-miR-27a 9.25 8.77 9.82 0.96 100 2.53E−03 −2.41 5.3 hsa-asg-924 4.184.00 4.25 0.55 0 3.29E−03 −2.41 5.3 hsa-asg-3376 4.25 3.62 3.99 0.34 07.01E−05 −2.41 5.3 hsa-miR-361 9.63 9.09 10.2 1.03 100 3.83E−03 −2.415.3 hsa-asg-14233 8.59 7.53 8.30 0.56 100 2.17E−04 −2.40 5.3hsa-asg-8174 3.98 2.88 3.28 0.51 0 2.78E−04 −2.38 5.2 hsa-asg-2837 2.492.29 2.87 0.63 0 8.13E−03 −2.34 5.1 hsa-asg-10674 5.47 4.98 4.99 0.33 04.04E−05 −2.33 5.0 hsa-asg-14146 4.63 3.92 4.65 0.57 0 9.04E−04 −2.325.0 hsa-miR-376b 2.81 2.94 2.66 0.33 0 2.39E−03 −2.29 4.9 hsa-miR-2113.63 2.12 3.24 0.75 0 3.73E−02 −2.28 4.9 hsa-miR-154* 3.67 3.76 4.370.80 0 2.06E−03 −2.23 4.7 hsa-asg-259 3.50 3.05 3.41 0.35 0 7.45E−05−2.23 4.7 hsa-asg-1596 3.87 3.48 3.71 0.44 0 1.71E−04 −2.22 4.7hsa-asg-13646 5.18 6.32 6.55 1.09 75 9.11E−03 −2.19 4.6 hsa-asg-141754.05 4.42 4.66 0.72 0 1.72E−03 −2.18 4.5 hsa-asg-4439 4.25 2.59 3.520.69 0 2.18E−02 −2.18 4.5 hsa-miR-99a 10.59 10.1 10.9 0.88 100 2.85E−03−2.15 4.5 hsa-miR-99a 10.83 10.3 11.2 0.92 100 9.19E−03 −1.74 3.3hsa-miR-499 3.12 3.38 3.72 0.96 0 1.88E−02 −2.15 4.4 hsa-miR-499 3.833.48 3.98 0.61 0 8.28E−03 −2.03 4.1 hsa-asg-3145 5.45 3.38 4.52 0.93 06.59E−03 −2.14 4.4 hsa-miR-504 3.22 4.00 3.64 0.36 0 4.63E−04 −2.13 4.4hsa-miR-489 4.58 3.84 4.78 0.72 0 1.57E−03 −2.13 4.4 hsa-asg-12139 2.812.94 3.02 0.23 0 1.93E−04 −2.09 4.2 hsa-miR-149 3.67 4.80 4.26 0.82 03.26E−03 −2.08 4.2 hsa-asg-9198 1.97 1.96 2.39 0.50 0 1.40E−02 −2.06 4.2hsa-asg-9980 3.59 4.04 3.67 0.56 0 1.72E−03 −2.06 4.2 hsa-miR-409-5p5.64 5.93 5.66 0.28 25 1.45E−04 −2.06 4.2 hsa-asg-13705 3.98 2.94 3.190.55 0 6.45E−04 −2.05 4.1 hsa-asg-13270 2.87 3.48 3.62 0.58 0 4.73E−03−2.04 4.1 hsa-asg-2027 3.12 3.67 3.16 0.70 0 4.71E−03 −2.04 4.1hsa-asg-360 3.22 3.67 3.77 0.42 0 8.72E−04 −2.04 4.1 hsa-miR-148b 7.156.55 7.96 1.31 100 2.27E−02 −2.03 4.1 hsa-asg-13011 3.91 3.05 3.81 0.590 1.02E−03 −2.03 4.1 hsa-miR-576 3.02 3.57 3.11 0.32 0 1.27E−02 −2.024.1 hsa-asg-11361 2.92 3.27 3.13 0.21 0 2.73E−05 −2.02 4.0 hsa-miR-148a8.93 8.31 9.61 1.21 100 1.83E−02 −2.02 4.0 hsa-cand207 3.32 3.43 3.490.34 0 7.06E−04 −2.00 4.0 hsa-miR-34b 5.10 3.27 4.46 0.85 0 5.38E−03−1.99 4.0 hsa-asg-13258 6.41 7.00 7.33 0.80 100 4.72E−03 −1.98 3.9hsa-asg-2301 5.51 5.43 5.82 0.42 100 5.19E−04 −1.96 3.9 hsa-miR-421 7.077.13 7.68 0.70 100 2.01E−03 −1.95 3.9 hsa-miR-421 6.56 6.40 6.91 0.52100 1.04E−03 −1.91 3.8 hsa-asg-8344 3.02 3.57 3.60 0.69 0 2.29E−03 −1.953.9 hsa-miR-10a 9.69 8.97 10.2 1.10 100 1.30E−02 −1.95 3.9 hsa-asg-23654.34 4.08 4.82 0.71 0 4.02E−03 −1.95 3.9 hsa-asg-10247 3.59 3.16 3.240.23 0 2.71E−04 −1.94 3.8 hsa-asg-10626 3.76 3.11 3.99 0.70 0 2.64E−03−1.93 3.8 hsa-asg-13997 3.67 3.27 3.19 0.40 0 1.67E−02 −1.92 3.8hsa-miR-493-3p 3.12 5.02 4.27 0.85 0 7.56E−03 −1.91 3.8 hsa-miR-28 9.008.63 9.57 0.89 100 5.60E−03 −1.90 3.7 hsa-miR-340 4.18 2.94 4.24 1.00 01.64E−02 −1.89 3.7 hsa-miR-135b 3.12 2.71 3.21 0.58 0 1.08E−02 −1.88 3.7hsa-asg-10830 2.81 2.77 3.24 0.57 0 1.09E−03 −1.87 3.7 hsa-miR-585 3.803.16 3.34 0.31 0 1.16E−03 −1.87 3.6 hsa-asg-14138 3.67 4.48 4.66 0.78 07.46E−03 −1.82 3.5 hsa-asg-11199 10.11 9.80 10.7 0.96 100 1.20E−02 −1.813.5 hsa-miR-151 7.89 8.61 8.89 0.80 100 4.54E−03 −1.81 3.5 hsa-asg-84283.02 2.94 3.16 0.24 0 2.95E−03 −1.80 3.5 hsa-asg-4219 3.59 2.88 3.240.34 0 1.89E−04 −1.79 3.5 hsa-miR-324-3p 6.29 6.95 7.03 0.55 0 1.85E−03−1.77 3.4 hsa-miR-422a 7.20 7.59 7.67 0.63 100 1.97E−03 −1.77 3.4hsa-asg-2967 3.22 3.67 3.63 0.34 0 2.16E−04 −1.76 3.4 hsa-miR-34a 6.827.21 7.92 1.07 100 1.85E−02 −1.75 3.4 hsa-miR-100 11.06 10.7 11.4 0.69100 2.43E−03 −1.74 3.3 hsa-miR-100 11.11 10.8 11.4 0.63 100 5.10E−03−1.37 2.6 hsa-miR-30e-3p 6.10 6.00 6.93 1.07 100 2.05E−02 −1.72 3.3hsa-asg-8878 8.21 7.85 8.84 0.98 100 2.16E−02 −1.70 3.3 hsa-miR-422b8.75 9.17 9.28 0.58 100 1.88E−03 −1.69 3.2 hsa-miR-542-3p 6.00 5.52 5.860.23 75 1.33E−04 −1.69 3.2 hsa-asg-10336 3.02 3.16 3.63 0.64 0 3.30E−03−1.69 3.2 hsa-miR-561 3.22 2.82 3.10 0.19 0 3.01E−02 −1.68 3.2hsa-miR-141 4.12 3.88 4.48 0.62 0 3.11E−02 −1.68 3.2 hsa-asg-3967 6.405.65 6.53 0.67 75 2.75E−03 −1.68 3.2 hsa-asg-280 3.12 3.16 3.19 0.05 01.18E−04 −1.67 3.2 hsa-asg-548 2.71 2.94 3.25 0.53 0 5.70E−03 −1.66 3.2hsa-miR-628 6.30 5.93 6.59 0.59 100 3.26E−03 −1.66 3.1 hsa-miR-452* 3.023.05 3.04 0.05 0 9.98E−03 −1.65 3.1 hsa-miR-193a 4.42 3.88 4.17 0.28 04.73E−04 −1.64 3.1 hsa-cand26 5.62 4.39 5.30 0.66 0 1.13E−02 −1.63 3.1hsa-cand720 5.35 4.48 5.11 0.60 0 3.01E−03 −1.63 3.1 hsa-asg-5715 4.683.48 4.67 0.94 0 1.54E−02 −1.61 3.1 hsa-miR-331 5.83 5.94 6.29 0.55 252.24E−03 −1.60 3.0 hsa-miR-331 6.48 6.86 7.51 1.04 100 2.64E−02 −1.583.0 hsa-asg-10391 4.79 4.42 4.72 0.22 0 8.86E−03 −1.60 3.0 hsa-asg-113803.02 3.16 3.16 0.22 0 1.84E−02 −1.58 3.0 hsa-miR-324-5p 7.23 7.71 7.600.34 100 5.76E−04 −1.57 3.0 hsa-asg-6758 3.50 2.53 3.25 0.64 0 5.71E−03−1.57 3.0 hsa-asg-8380 3.67 3.38 3.96 0.67 0 1.95E−02 −1.54 2.9hsa-cand330 2.71 3.67 3.77 0.78 0 2.05E−02 −1.51 2.9 hsa-asg-13279 5.585.92 6.33 0.71 50 6.44E−03 −1.51 2.9 hsa-asg-11181 5.41 4.22 5.02 0.56 06.95E−03 −1.51 2.9 hsa-miR-338 3.98 3.57 3.97 0.30 0 8.30E−03 −1.51 2.8hsa-miR-186 5.51 5.36 6.16 0.87 75 1.58E−02 −1.51 2.8 hsa-asg-3811 10.339.15 10.3 0.85 100 1.84E−02 −1.47 2.8 hsa-asg-13406 2.92 3.80 3.50 0.440 7.74E−03 −1.46 2.8 hsa-asg-12465 3.91 3.27 4.08 0.62 0 4.00E−03 −1.462.7 hsa-asg-3902 3.41 3.43 3.55 0.35 0 5.58E−04 −1.45 2.7 hsa-asg-118774.12 4.29 4.10 0.22 0 2.91E−02 −1.45 2.7 hsa-miR-143 11.23 10.8 11.60.80 100 1.28E−02 −1.42 2.7 hsa-asg-3998 7.10 7.17 7.46 0.59 1005.05E−03 −1.42 2.7 hsa-asg-10278 3.72 3.00 3.68 0.48 25 5.82E−03 −1.412.7 hsa-asg-4122 4.94 4.22 4.94 0.53 0 2.83E−02 −1.41 2.7 hsa-asg-101134.68 3.67 3.94 0.58 0 1.25E−02 −1.41 2.7 hsa-miR-128b 8.52 8.57 9.310.88 100 2.05E−02 −1.41 2.6 hsa-asg-9723 3.59 2.71 3.09 0.40 0 4.60E−02−1.40 2.6 hsa-miR-128a 8.18 8.13 8.86 0.83 100 1.71E−02 −1.38 2.6hsa-miR-31 9.90 10.7 10.5 0.64 100 2.62E−02 −1.38 2.6 hsa-asg-14048 3.594.51 4.18 0.46 0 1.67E−03 −1.38 2.6 hsa-asg-11906 4.34 4.29 4.27 0.34 07.39E−03 −1.37 2.6 hsa-asg-9473 4.94 6.41 5.79 0.85 50 3.78E−02 −1.362.6 hsa-miR-30d 11.28 10.8 11.7 0.75 100 1.21E−02 −1.35 2.6hsa-asg-12083 5.54 3.80 4.68 0.72 0 1.69E−02 −1.35 2.6 hsa-miR-30a-5p10.52 10.2 11.0 0.80 100 1.63E−02 −1.35 2.5 hsa-miR-425-3p 5.00 5.115.76 0.91 50 3.70E−02 −1.34 2.5 hsa-asg-12346 6.70 5.94 6.64 0.50 09.46E−03 −1.34 2.5 hsa-miR-98 10.09 9.44 10.3 0.73 100 1.12E−02 −1.342.5 hsa-asg-5304 8.42 8.80 9.32 0.94 100 3.22E−02 −1.33 2.5hsa-asg-11285 3.98 3.67 4.05 0.34 0 4.18E−04 −1.33 2.5 hsa-asg-153 3.913.27 3.39 0.79 0 3.56E−02 −1.33 2.5 hsa-miR-212 5.06 4.98 5.42 0.53 01.97E−02 −1.32 2.5 hsa-asg-13135 5.51 4.72 5.52 0.57 0 2.67E−02 −1.302.5 hsa-asg-13308 8.26 8.68 9.20 0.94 100 3.58E−02 −1.30 2.5hsa-asg-4542 4.58 3.53 4.40 0.59 0 8.62E−03 −1.29 2.4 hsa-asg-8266 3.834.04 4.16 0.27 0 5.52E−03 −1.29 2.4 hsa-asg-9848 4.18 4.65 4.31 0.42 01.92E−02 −1.28 2.4 hsa-asg-5873 5.30 4.22 5.08 0.73 25 3.37E−02 −1.282.4 hsa-asg-7803 3.22 3.96 3.87 0.46 0 3.26E−02 −1.28 2.4 hsa-asg-30385.15 4.65 4.99 0.25 0 9.03E−04 −1.26 2.4 hsa-miR-320 10.46 10.6 11.00.57 100 5.70E−03 −1.25 2.4 hsa-miR-598 5.45 4.85 5.65 0.71 25 2.39E−02−1.25 2.4 hsa-miR-30e-5p 8.15 8.76 9.11 0.89 100 3.72E−02 −1.24 2.4hsa-asg-13661 4.79 5.15 5.11 0.33 0 2.54E−02 −1.23 2.4 hsa-let-7e 11.8310.8 11.8 0.71 100 1.65E−02 −1.20 2.3 hsa-asg-1399 3.83 3.96 4.19 0.49 07.79E−03 −1.19 2.3 hsa-asg-12403 3.91 4.48 4.66 0.58 0 3.08E−02 −1.192.3 hsa-asg-1782 3.22 2.82 3.16 0.53 0 8.92E−03 −1.18 2.3 hsa-asg-84767.05 6.23 6.48 0.67 0 1.55E−02 −1.17 2.3 hsa-miR-197 8.77 7.76 8.90 0.85100 4.57E−02 −1.17 2.2 hsa-miR-24 11.95 11.8 12.4 0.60 100 8.35E−03−1.17 2.2 hsa-asg-5973 4.90 4.35 4.96 0.54 25 7.81E−03 −1.15 2.2hsa-asg-5740 4.75 3.76 4.49 0.60 0 1.39E−02 −1.15 2.2 hsa-miR-376a* 3.834.08 4.44 0.58 0 8.06E−03 −1.15 2.2 hsa-asg-3614 3.95 4.22 4.14 0.14 01.59E−02 −1.14 2.2 hsa-miR-641 3.50 3.27 3.34 0.12 0 3.28E−02 −1.13 2.2hsa-asg-2596 3.41 4.19 4.23 0.61 0 4.48E−02 −1.12 2.2 hsa-asg-2992 5.494.42 4.89 0.48 0 2.37E−02 −1.11 2.2 hsa-miR-30a-3p 7.33 7.35 7.89 0.67100 2.91E−02 −1.11 2.2 hsa-asg-3604 5.24 4.00 5.20 0.85 50 4.59E−02−1.11 2.2 hsa-miR-454-3p 7.07 7.03 7.61 0.66 100 2.21E−02 −1.10 2.1hsa-asg-8737 7.61 8.06 7.78 0.19 0 3.62E−04 −1.09 2.1 hsa-asg-8354 3.413.88 3.75 0.25 0 3.16E−02 −1.07 2.1 hsa-asg-12998 3.59 4.35 3.96 0.35 02.44E−02 −1.07 2.1 hsa-asg-9815 4.86 5.36 5.36 0.37 0 2.08E−03 −1.07 2.1hsa-asg-5053 5.54 4.70 5.39 0.47 50 1.12E−02 −1.06 2.1 hsa-asg-139053.59 3.53 3.82 0.42 0 4.50E−02 −1.05 2.1 hsa-asg-11397 3.76 3.48 3.610.25 0 1.98E−02 −1.05 2.1 hsa-let-7b 13.0 12.3 13.3 0.82 100 4.62E−02−1.03 2.0 hsa-miR-510 4.31 4.12 4.48 0.35 0 1.54E−03 −1.03 2.0hsa-asg-14015 4.88 4.54 4.89 0.33 0 2.74E−02 −1.03 2.0 SD, standarddeviation; %, percentage of samples expressing miRNA above backgroundlevels; Log2 Diff, difference in VSN-transformed expression between NCXand LNneg samples.

Example 3 miRNAs Differentially Expressed Between Cancer-positive andCancer-negative Inguinal Lymph Nodes from Cervical Cancer Patients

To identify miRNAs that may be useful markers for metastasis of cervicalcancer to inguinal lymph nodes, the inventors identified miRNAs that areexpressed at significantly higher levels in cancer-positive inguinallymph nodes from cervical cancer patients than in cancer-negativeinguinal lymph nodes.

miRNA expression was determined for four cancer-positive lymph nodes(LNpos1-4) and four cancer-negative lymph nodes (LNneg1-4) isolated fromeight different cervical cancer patients (described in Example 1, Table3). miRNAs were purified from formalin-fixed, paraffin-embedded (FFPE)lymph nodes as described in Example 1 and analyzed with microarrays asdescribed in Example 2.

A total of 126 miRNAs (39 verified, 87 predicted) exhibited significantdifferential expression between cancer-negative lymph nodes (LNneg) andcancer-positive lymph nodes (LNpos), (t-test (LNpos vs LNneg)<0.05 andlog 2-Diff (LNpos vs LNneg)≦−1 (Table 5)). Among the verified miRNAs,the average expression level of hsa-miR-205 was more than 1.000-foldlower in LNneg samples than in LNpos samples; the average expressionlevels of three miRNAs (hsa-miR-203, -200b, and -200a) were between 100-and 1.000-fold lower in LNneg samples; the average expression levels oftwo miRNAs (hsa-miR-429 and -200c) were between 50- and 100-fold lowerin LNneg samples; the average expression levels of six miRNAs(hsa-miR-224, -141, -200a*, -183, -605, and -182) were between 10- and50-fold lower in LNneg samples; and the average expression levels ofeight miRNAs (hsa-miR-9, -493-5p, -9*, -495, -576, -368, -452, and -189)were between 5- and 10-fold lower in LNneg samples than in LNpossamples. Among the 87 predicted miRNAs, the average expression levels oftwo (hsa-asg-14153_st2, and -7637_st2) were at least 45-fold lower inLNneg samples than in LNpos samples; the average expression levels oftwo other miRNAs (hsa-asg-2919_st1, and -12227_st1) were between 10- and45-fold lower in LNneg samples; and the average expression levels of 14miRNAs (hsa-asg-11715_st2, -11160_st2, -5119_st1, -1296_st1, -10131_st2,-14176_st1, -8174_st1, -2785_st2, -4978_st2, -9198_st1, -8428_st2,-9112_st2, -1596_st2, -12139-st2) were between 5- and 10-fold lower inLNneg samples than in LNpos samples.

TABLE 5 MicroRNAs Differentially Expressed Between Cancer-PositiveInguinal Lymph Node Samples (LNpos) and Cancer-Negative Inguinal LymphNode Samples (LNneg). LNpos LNneg Micro RNA Probe ID 1 2 3 4 Mean SD % 12 hsa-miR-205 hsa-miR-205_st1 13.8 13.2 13.1 13.2 13.36 0.32 100 3.612.06 hsa-miR-203 hsa-miR-203_st2 13.5 13.2 12.9 11.1 12.73 1.08 100 4.945.50 hsa-miR-200b hsa-miR-200b_st1 13.6 13.3 13.1 13.5 13.42 0.20 1006.68 7.72 hsa-miR-200a hsa-miR-200a_st2 11.61 0.7 11.1 11.5 11.26 0.43100 4.99 5.73 hsa-asg-14153 hsa-asg-14153_st2 9.33 8.44 8.83 9.56 9.040.50 100 2.97 2.65 hsa-miR-429 hsa-miR-429_st1 9.19 8.39 9.96 9.73 9.320.70 100 3.22 3.11 hsa-miR-200c hsa-miR-200c_st1 13.5 13.0 13.1 13.113.19 0.24 100 7.89 8.26 hsa-asg-7637 hsa-asg-7637_st2 9.45 8.87 9.159.99 9.37 0.48 100 3.51 4.37 hsa-miR-224 hsa-miR-224_st2 11.4 11.2 11.211.3 11.35 0.10 100 7.29 8.21 hsa-miR-224 hsa-asg-14247_st1 11.5 11.511.4 11.5 11.53 0.05 100 7.41 8.28 hsa-miR-141 hsa-miR-141_st1 9.72 7.998.86 8.55 8.78 0.72 100 4.64 5.28 hsa-miR-200a* hsa-miR-200a-_st2 8.086.62 7.62 7.59 7.48 0.61 100 3.16 3.47 hsa-miR-183 hsa-miR-183_st1 9.918.71 9.49 9.85 9.49 0.55 100 6.58 5.76 hsa-asg-2919 hsa-asg-2919_st18.25 6.55 6.54 5.60 6.73 1.10 75 2.97 2.45 hsa-asg-12227hsa-asg-12227_st1 7.19 4.29 6.50 6.63 6.15 1.28 75 2.91 2.32 hsa-miR-605hsa-miR-605_st1 6.71 5.79 6.28 6.09 6.22 0.38 75 2.25 2.85 hsa-miR-182hsa-miR-182_st1 11.0 10.0 10.7 11.1 10.75 0.47 100 8.42 8.10hsa-asg-11715 hsa-asg-11715_st2 6.65 6.23 5.56 5.80 6.06 0.48 100 2.782.85 hsa-asg-11160 hsa-asg-11160_st2 6.27 6.04 5.55 6.04 5.97 0.30 502.91 2.45 hsa-miR-9 hsa-miR-9_st2 4.82 6.90 6.93 7.78 6.61 1.26 75 3.773.78 hsa-miR-9 hsa-cand90_st2 4.51 5.52 6.10 6.83 5.74 0.98 50 2.97 3.35hsa-miR-493-5p hsa-miR-493-5p_st1 7.45 5.05 7.02 5.87 6.35 1.09 75 3.344.14 hsa-asg-5119 hsa-asg-5119_st1 6.47 5.97 5.41 5.96 5.95 0.43 75 3.342.45 hsa-asg-1296 hsa-asg-1296_st1 6.98 5.46 6.61 6.48 6.38 0.65 75 3.223.11 hsa-asg-10131 hsa-asg-10131_st2 6.82 6.18 5.17 6.05 6.05 0.68 1002.71 3.24 hsa-asg-14176 hsa-asg-14176_st1 9.20 6.64 5.17 7.76 7.19 1.7175 4.76 5.47 hsa-asg-8174 hsa-asg-8174_st1 5.95 6.61 5.79 6.41 6.19 0.3975 2.91 3.35 hsa-miR-9* hsa-miR-9-_st2 6.19 8.00 7.59 8.60 7.60 1.02 1005.26 5.81 hsa-miR-495 hsa-miR-495_st1 6.58 4.42 6.42 5.53 5.74 1.00 503.22 2.72 hsa-miR-576 hsa-miR-576_st2 6.37 6.54 6.03 4.36 5.82 1.00 752.84 2.98 hsa-asg-2785 hsa-asg-2785_st2 5.63 6.36 8.20 6.55 6.69 1.08 504.12 3.47 hsa-asg-4978 hsa-asg-4978_st2 5.30 5.93 4.58 6.04 5.46 0.67 502.58 3.35 hsa-miR-368 hsa-miR-368_st2 7.55 4.83 7.40 6.27 6.51 1.26 754.35 4.22 hsa-asg-9198 hsa-asg-9198_st1 6.12 5.34 5.37 3.31 5.03 1.20 502.91 2.72 hsa-miR-452 hsa-miR-452_st1 7.53 7.45 7.96 7.68 7.65 0.22 1005.64 5.36 hsa-asg-8428 hsa-asg-8428_st2 6.56 6.21 5.76 4.30 5.71 0.99 753.22 3.47 hsa-asg-9112 hsa-asg-9112_st2 7.76 7.84 10.2 8.42 8.57 1.16100 6.21 5.47 hsa-miR-189 hsa-miR-189_st1 6.90 6.61 5.94 5.71 6.29 0.5675 3.81 4.70 hsa-asg-1596 hsa-asg-1596_st2 6.60 6.06 5.83 6.09 6.14 0.3350 4.24 3.24 hsa-asg-12139 hsa-asg-12139_st2 5.60 6.27 4.24 5.26 5.340.84 25 2.97 3.35 hsa-miR-422a hsa-miR-422a_st1 10.2 9.42 10.1 9.92 9.950.38 100 7.31 8.59 hsa-miR-96 hsa-miR-96_st1 6.06 4.83 5.78 5.69 5.590.53 75 3.45 3.24 hsa-asg-7471 hsa-asg-7471_st1 8.18 8.00 7.33 7.15 7.670.50 100 5.32 5.84 hsa-asg-10154 hsa-asg-10154_st1 6.07 6.89 9.12 7.247.33 1.29 25 4.73 4.78 hsa-asg-3711 hsa-asg-3711_st2 6.76 6.05 6.68 7.226.68 0.48 75 4.49 4.70 hsa-asg-12729 hsa-asg-12729_st1 7.57 7.95 10.28.07 8.46 1.21 100 5.93 6.27 hsa-asg-11361 hsa-asg-11361_st2 5.58 5.644.95 5.20 5.34 0.33 75 3.34 2.98 hsa-asg-9980 hsa-asg-9980_st1 6.08 5.016.29 5.89 5.82 0.56 75 2.91 4.14 hsa-asg-5021 hsa-asg-5021_st1 6.42 3.695.78 5.84 5.43 1.19 50 4.04 3.24 hsa-miR-27b hsa-miR-27b_st1 13.2 12.712.7 12.3 12.78 0.38 100 11.2 11.4 hsa-miR-199b hsa-miR-199b_st2 9.367.82 9.35 8.73 8.82 0.73 100 6.16 7.61 hsa-asg-924 hsa-asg-924_st1 6.636.71 5.09 7.00 6.36 0.86 75 5.04 3.78 hsa-asg-10247 hsa-asg-10247_st15.60 4.96 5.55 5.23 5.34 0.30 25 3.10 3.11 hsa-asg-3998 hsa-asg-3998_st19.84 9.03 9.74 9.59 9.55 0.36 100 7.22 8.34 hsa-asg-548 hsa-asg-548_st15.52 5.77 4.24 5.71 5.31 0.72 25 3.45 3.88 hsa-miR-561 hsa-miR-561_st15.23 6.27 4.72 4.42 5.16 0.81 25 3.10 3.24 hsa-miR-422b hsa-miR-422b_st211.51 0.8 11.5 11.2 11.30 0.32 100 9.09 10.1 hsa-miR-127 hsa-miR-127_st18.40 6.92 8.04 7.67 7.76 0.63 100 6.07 5.95 hsa-asg-13908hsa-asg-13908_st2 6.06 4.35 5.27 5.64 5.33 0.73 75 2.97 3.97hsa-asg-13705 hsa-asg-13705_st1 5.55 4.79 5.06 5.13 5.13 0.31 25 3.102.72 hsa-asg-12762 hsa-asg-12762_st2 6.53 5.72 5.71 6.36 6.08 0.43 1003.22 3.68 hsa-asg-13997 hsa-asg-13997_st2 5.58 5.69 5.03 4.18 5.12 0.6975 2.71 3.11 hsa-miR-432 hsa-miR-432_st2 6.50 5.01 5.73 5.71 5.74 0.6150 4.08 3.68 hsa-miR-494 hsa-miR-494_st1 7.86 7.01 7.63 7.12 7.40 0.41100 5.33 5.47 hsa-miR-494 hsa-miR61_st2 7.17 6.45 6.89 7.21 6.93 0.35100 5.51 5.36 hsa-miR154 hsa-miR154_st1 5.95 5.82 6.74 6.02 6.13 0.41100 4.42 5.01 hsa-asg-8079 hsa-asg-8079_st1 5.94 5.74 6.32 5.67 5.920.29 25 3.86 3.52 hsa-miR-210 hsa-miR-210_st1 9.60 8.95 9.02 8.90 9.120.33 100 7.20 7.81 hsa-asg-13770 hsa-asg-13770_st1 5.46 5.16 7.73 5.345.92 1.21 25 3.86 4.30 hsa-asg-13254 hsa-asg-13254_st1 6.23 3.69 6.195.93 5.51 1.22 75 4.45 3.47 hsa-asg-54 hsa-asg-54_st2 5.43 4.23 5.214.85 4.93 0.52 25 2.51 2.85 hsa-asg-2967 hsa-asg-2967_st2 5.84 5.46 5.195.02 5.38 0.36 50 3.56 4.06 hsa-asg-128 hsa-asg-128_st2 6.02 4.88 5.704.81 5.35 0.60 25 2.78 4.37 hsa-cand271 hsa-cand271_st2 4.91 5.14 6.695.64 5.60 0.79 25 4.28 2.85 hsa-asg-14146 hsa-asg-14146_st1 5.71 5.585.97 7.96 6.31 1.12 75 4.73 5.32 hsa-miR-376a hsa-miR-376a_st2 6.19 4.535.63 4.98 5.33 0.73 50 3.67 3.97 hsa-asg-9723 hsa-asg-9723_st2 5.33 5.164.54 3.90 4.73 0.65 50 2.84 3.24 hsa-asg-11969 hsa-asg-11969_st1 8.177.19 7.31 7.71 7.59 0.44 100 5.76 5.65 hsa-asg-10577 hsa-asg-10577_st17.00 7.04 8.97 7.43 7.61 0.93 100 5.90 6.02 hsa-asg-5998hsa-asg-5998_st1 6.31 6.45 8.44 6.61 6.95 1.00 75 5.30 5.17 hsa-miR-34bhsa-miR-34b_st2 5.91 6.43 5.49 6.39 6.06 0.45 100 5.04 4.44 hsa-asg-8450hsa-asg-8450_st1 6.77 5.96 6.82 6.71 6.56 0.40 75 4.99 4.44 hsa-asg-8266hsa-asg-8266_st2 6.21 6.27 4.96 5.42 5.71 0.63 50 4.31 4.44 hsa-cand138hsa-cand138_st1 4.39 3.78 6.19 3.49 4.46 1.21 25 2.91 2.85 hsa-asg-9405hsa-asg-9405_st1 6.69 7.03 8.05 6.61 7.10 0.66 100 5.39 5.60hsa-miR-299-5p hsa-miR-299-5p_st2 6.12 3.94 4.85 4.67 4.90 0.90 25 4.043.47 hsa-asg-1929 hsa-asg-1929_st1 4.27 4.23 4.69 4.58 4.44 0.23 25 3.343.11 hsa-asg-9702 hsa-asg-9702_st2 5.04 4.16 5.34 3.97 4.63 0.66 25 3.223.24 hsa-asg-7033 hsa-asg-7033_st1 6.37 6.18 6.85 5.73 6.28 0.46 1004.87 4.44 hsa-miR-130b hsa-miR-130b_st1 9.33 9.00 9.68 9.65 9.41 0.32100 8.39 8.14 hsa-miR-27a hsa-miR-27a_st2 11.7 11.0 11.3 10.9 11.28 0.34100 10.4 10.7 hsa-asg-9186 hsa-asg-9186_st1 5.66 5.65 7.32 5.73 6.090.82 25 4.67 4.51 hsa-asg-1399 hsa-asg-1399_st1 5.99 6.27 4.40 5.61 5.570.82 25 4.92 4.06 hsa-asg-11381 hsa-asg-11381_st2 6.23 6.74 6.65 7.016.66 0.32 100 4.87 5.06 hsa-miR-31 hsa-miR-31_st2 11.9 12.3 11.2 12.011.90 0.44 100 11.3 10.1 hsa-asg-6180 hsa-asg-6180_st1 7.13 7.25 7.837.78 7.50 0.36 100 6.63 6.14 hsa-asg-3383 hsa-asg-3383_st1 7.70 7.689.30 7.98 8.16 0.77 100 6.75 6.90 hsa-asg-13258 hsa-asg-13258_st2 9.277.83 8.31 9.06 8.62 0.67 100 7.64 8.26 hsa-asg-11877 hsa-asg-11877_st15.11 5.03 6.26 5.13 5.38 0.59 25 4.20 3.78 hsa-asg-14048hsa-asg-14048_st1 5.69 5.40 5.50 5.26 5.46 0.18 25 4.58 4.06hsa-asg-14233 hsa-asg-14233_st1 9.98 9.59 9.55 9.17 9.57 0.33 100 8.288.80 hsa-asg-3884 hsa-asg-3884_st1 5.66 4.74 5.43 5.97 5.45 0.52 25 3.953.47 hsa-miR-382 hsa-miR-382_st1 7.88 6.12 7.35 7.39 7.19 0.75 75 6.246.05 hsa-asg-6788 hsa-asg-6788_st1 7.11 6.75 8.38 6.47 7.18 0.84 75 5.935.32 hsa-asg-14237 hsa-asg-14237_st2 7.77 6.85 7.11 6.59 7.08 0.51 1006.23 5.84 hsa-asg-14177 hsa-asg-14177_st1 3.99 4.02 5.03 3.01 4.01 0.8225 2.71 2.59 hsa-miR-134 hsa-miR-134_st2 6.32 5.07 6.07 5.33 5.70 0.5925 4.79 4.51 hsa-asg-14137 hsa-asg-14137_st1 6.27 6.42 7.33 6.77 6.700.47 100 5.50 4.64 hsa-asg-4223 hsa-asg-4223_st2 8.26 7.96 8.54 8.738.37 0.33 100 7.90 7.13 hsa-asg-6341 hsa-asg-6341_st2 9.67 9.12 10.29.78 9.70 0.45 100 8.86 8.14 hsa-asg-13205 hsa-asg-13205_st1 6.94 6.196.92 5.26 6.33 0.79 75 5.24 5.76 hsa-asg-9729 hsa-asg-9729_st2 7.16 6.745.90 6.83 6.66 0.53 100 5.94 6.00 hsa-asg-13278 hsa-asg-13278_st2 10.19.60 10.0 9.99 9.95 0.25 100 9.36 8.71 hsa-asg-10199 hsa-asg-10199_st16.66 5.72 7.14 6.48 6.50 0.59 50 5.33 5.34 hsa-asg-9126 hsa-asg-9126_st25.84 5.35 6.53 5.23 5.74 0.59 25 4.42 4.51 hsa-asg-7568 hsa-asg-7568_st16.78 6.40 6.91 6.76 6.71 0.22 75 6.03 4.84 hsa-asg-13841 hsa-asg-13841_st2 7.08 6.79 7.12 7.08 7.02 0.15 100 6.21 5.36 hsa-asg-11285hsa-asg-11285_st2 5.79 5.91 4.69 4.30 5.17 0.80 50 4.49 4.06hsa-asg-4339 hsa-asg-4339_st2 6.88 5.83 6.78 6.89 6.60 0.51 75 5.98 5.11hsa-asg-12146 hsa-asg-12146_st2 6.17 5.84 5.88 5.60 5.87 0.23 25 4.495.04 hsa-asg-9146 hsa-asg-9146_st1 8.69 8.67 9.55 8.96 8.97 0.41 25 8.137.55 hsa-asg-12376 hsa-asg-12376_st2 5.76 5.55 6.92 5.98 6.05 0.60 254.87 4.37 hsa-asg-542 hsa-asg-542_st1 8.14 8.20 9.29 8.11 8.44 0.57 1007.65 6.89 hsa-asg-8003 hsa-asg-8003_st2 6.50 6.89 7.09 6.77 6.81 0.25100 5.35 6.00 hsa-asg-9227 hsa-asg-9227_st1 7.18 6.96 8.42 6.70 7.310.76 100 6.47 6.21 hsa-cand720 hsa-cand720_st1 6.58 5.86 6.29 5.82 6.140.36 50 4.79 5.83 hsa-asg-2858 hsa-asg-2858_st1 7.10 6.58 6.93 7.23 6.960.28 100 6.14 5.24 hsa-miR-93 hsa-miR-93_st2 11.41 1.11 1.21 1.01 1.220.17 100 10.6 10.5 hsa-asg-3631 hsa-asg-3631_st1 7.76 7.83 8.31 7.507.85 0.34 75 6.63 6.86 LNneg vs LNpos LNneg Log2 Fold Micro RNA 3 4 MeanSD % Ttest Diff change hsa-miR-205 2.65 3.57 2.98 0.75 0 2.48E−07 −10.381332 hsa-miR-203 3.59 4.15 4.55 0.84 0 2.12E−05 −8.18 290.1 hsa-miR-200b5.92 6.17 6.62 0.80 100 3.11E−06 −6.80 111.6 hsa-miR-200a 3.83 3.48 4.511.04 25 2.01E−05 −6.76 108.1 hsa-asg-14153 2.71 2.59 2.73 0.17 03.59E−07 −6.31 79.2 hsa-miR-429 3.59 3.38 3.33 0.21 0 3.19E−06 −5.9963.7 hsa-miR-200c 7.04 6.42 7.40 0.83 100 1.08E−05 −5.79 55.3hsa-asg-7637 4.09 3.05 3.75 0.59 0 6.02E−06 −5.61 48.9 hsa-miR-224 5.675.82 6.75 1.22 50 2.89E−04 −4.60 24.3 hsa-miR-224 5.81 6.49 7.00 1.08100 1.55E−04 −4.54 23.2 hsa-miR-141 4.12 3.88 4.48 0.62 0 1.03E−04 −4.3019.7 hsa-miR-200a* 3.67 2.77 3.27 0.40 0 2.54E−05 −4.21 18.5 hsa-miR-1834.31 5.77 5.60 0.95 25 3.96E−04 −3.88 14.8 hsa-asg-2919 3.50 2.71 2.910.45 0 6.72E−04 −3.82 14.2 hsa-asg-12227 2.12 2.35 2.43 0.34 0 1.33E−03−3.73 13.2 hsa-miR-605 3.02 2.53 2.66 0.34 0 8.95E−06 −3.55 11.7hsa-miR-182 5.76 7.23 7.38 1.19 100 1.87E−03 −3.37 10.3 hsa-asg-117153.22 2.18 2.76 0.43 0 5.11E−05 −3.30 9.9 hsa-asg-11160 2.87 2.71 2.730.21 0 2.02E−06 −3.24 9.4 hsa-miR-9 2.60 3.92 3.52 0.62 0 4.55E−03 −3.098.5 hsa-miR-9 3.12 3.16 3.15 0.16 0 1.97E−03 −2.59 6.0 hsa-miR-493-5p2.38 3.27 3.28 0.72 0 3.39E−03 −3.06 8.4 hsa-asg-5119 3.59 2.24 2.900.66 0 2.49E−04 −3.05 8.3 hsa-asg-1296 4.12 2.94 3.35 0.53 0 3.46E−04−3.03 8.2 hsa-asg-10131 3.50 2.71 3.04 0.40 0 2.59E−04 −3.01 8.1hsa-asg-14176 4.22 2.59 4.26 1.22 0 3.15E−02 −2.94 7.6 hsa-asg-8174 3.982.88 3.28 0.51 0 1.03E−04 −2.91 7.5 hsa-miR-9* 4.37 4.00 4.86 0.83 255.95E−03 −2.74 6.7 hsa-miR-495 3.12 2.94 3.00 0.22 0 1.72E−03 −2.74 6.7hsa-miR-576 3.02 3.57 3.11 0.32 0 2.06E−03 −2.72 6.6 hsa-asg-2785 4.314.00 3.98 0.36 0 3.16E−03 −2.71 6.5 hsa-asg-4978 2.28 2.82 2.76 0.46 05.61E−04 −2.70 6.5 hsa-miR-368 3.32 3.48 3.84 0.52 0 7.76E−03 −2.67 6.4hsa-asg-9198 1.97 1.96 2.39 0.50 0 6.65E−03 −2.65 6.3 hsa-miR-452 3.675.36 5.01 0.90 0 1.26E−03 −2.64 6.2 hsa-asg-8428 3.02 2.94 3.16 0.24 02.49E−03 −2.54 5.8 hsa-asg-9112 6.29 6.41 6.09 0.42 50 6.97E−03 −2.475.6 hsa-miR-189 3.17 3.67 3.84 0.63 0 1.15E−03 −2.45 5.5 hsa-asg-15963.87 3.48 3.71 0.44 0 1.13E−04 −2.44 5.4 hsa-asg-12139 2.81 2.94 3.020.23 0 1.81E−03 −2.32 5.0 hsa-miR-422a 7.20 7.59 7.67 0.63 100 8.43E−04−2.28 4.8 hsa-miR-96 3.32 3.27 3.32 0.09 0 1.49E−04 −2.27 4.8hsa-asg-7471 5.42 5.05 5.41 0.33 0 2.89E−04 −2.26 4.8 hsa-asg-10154 5.135.64 5.07 0.42 0 1.58E−02 −2.26 4.8 hsa-asg-3711 4.25 4.35 4.45 0.19 01.36E−04 −2.23 4.7 hsa-asg-12729 6.26 6.49 6.24 0.23 75 1.12E−02 −2.224.7 hsa-asg-11361 2.92 3.27 3.13 0.21 0 2.71E−05 −2.21 4.6 hsa-asg-99803.59 4.04 3.67 0.56 0 1.67E−03 −2.15 4.4 hsa-asg-5021 3.12 2.82 3.310.52 0 1.71E−02 −2.13 4.4 hsa-miR-27b 10.4 9.46 10.6 0.91 100 5.10E−03−2.11 4.3 hsa-miR-199b 6.54 6.50 6.71 0.63 100 4.63E−03 −2.11 4.3hsa-asg-924 4.18 4.00 4.25 0.55 0 6.20E−03 −2.10 4.3 hsa-asg-10247 3.593.16 3.24 0.23 0 3.30E−05 −2.10 4.3 hsa-asg-3998 7.10 7.17 7.46 0.59 1009.28E−04 −2.09 4.3 hsa-asg-548 2.71 2.94 3.25 0.53 0 3.54E−03 −2.07 4.2hsa-miR-561 3.22 2.82 3.10 0.19 0 2.59E−03 −2.06 4.2 hsa-miR-422b 8.759.17 9.28 0.58 100 9.07E−04 −2.02 4.1 hsa-miR-127 5.48 5.52 5.76 0.30 251.24E−03 −2.00 4.0 hsa-asg-13908 3.32 3.05 3.33 0.45 0 3.41E−03 −2.004.0 hsa-asg-13705 3.98 2.94 3.19 0.55 0 8.76E−04 −1.94 3.8 hsa-asg-127624.73 4.94 4.14 0.82 0 5.88E−03 −1.94 3.8 hsa-asg-13997 3.67 3.27 3.190.40 0 2.90E−03 −1.93 3.8 hsa-miR-432 2.81 4.67 3.81 0.78 0 8.09E−03−1.92 3.8 hsa-miR-494 5.10 6.09 5.50 0.43 50 6.35E−04 −1.91 3.8hsa-miR-494 5.74 6.05 5.67 0.30 75 1.56E−03 −1.27 2.4 hsa-miR154 3.673.84 4.24 0.61 0 2.13E−03 −1.89 3.7 hsa-asg-8079 4.15 4.59 4.03 0.45 04.20E−04 −1.89 3.7 hsa-miR-210 6.58 7.37 7.24 0.51 100 8.09E−04 −1.883.7 hsa-asg-13770 4.12 3.92 4.05 0.20 0 2.25E−02 −1.87 3.7 hsa-asg-132543.76 3.11 3.70 0.57 0 3.57E−02 −1.81 3.5 hsa-asg-54 3.91 3.22 3.12 0.600 3.93E−03 −1.80 3.5 hsa-asg-2967 3.22 3.67 3.63 0.34 0 4.06E−04 −1.753.4 hsa-asg-128 3.67 3.62 3.61 0.65 0 7.73E−03 −1.74 3.3 hsa-cand2714.18 4.22 3.88 0.69 0 1.72E−02 −1.71 3.3 hsa-asg-14146 4.63 3.92 4.650.57 0 3.85E−02 −1.66 3.1 hsa-miR-376a 3.83 3.27 3.69 0.30 0 5.92E−03−1.65 3.1 hsa-asg-9723 3.59 2.71 3.09 0.40 0 5.14E−03 −1.64 3.1hsa-asg-11969 6.01 6.42 5.96 0.34 25 1.11E−03 −1.63 3.1 hsa-asg-105775.94 6.09 5.99 0.09 50 1.31E−02 −1.62 3.1 hsa-asg-5998 5.41 5.49 5.340.14 0 1.88E−02 −1.61 3.1 hsa-miR-34b 5.10 3.27 4.46 0.85 0 1.58E−02−1.59 3.0 hsa-asg-8450 5.48 5.07 4.99 0.43 0 1.75E−03 −1.57 3.0hsa-asg-8266 3.83 4.04 4.16 0.27 0 4.08E−03 −1.56 2.9 hsa-cand138 2.603.32 2.92 0.30 0 4.81E−02 −1.54 2.9 hsa-asg-9405 5.58 5.75 5.58 0.15 04.22E−03 −1.52 2.9 hsa-miR-299-5p 2.71 3.38 3.40 0.55 0 2.97E−02 −1.502.8 hsa-asg-1929 2.49 2.82 2.94 0.37 0 4.43E−04 −1.50 2.8 hsa-asg-97023.41 2.71 3.14 0.30 0 6.62E−03 −1.48 2.8 hsa-asg-7033 5.10 4.82 4.810.27 0 1.52E−03 −1.48 2.8 hsa-miR-130b 7.53 7.73 7.95 0.39 100 1.14E−03−1.46 2.8 hsa-miR-27a 9.25 8.77 9.82 0.96 100 2.88E−02 −1.46 2.8hsa-asg-9186 4.77 4.80 4.69 0.13 0 1.50E−02 −1.40 2.6 hsa-asg-1399 3.833.96 4.19 0.49 0 2.87E−02 −1.37 2.6 hsa-asg-11381 5.59 5.65 5.29 0.39 01.64E−03 −1.37 2.6 hsa-miR-31 9.90 10.76 10.5 0.64 100 1.30E−02 −1.352.6 hsa-asg-6180 5.72 6.13 6.15 0.37 75 2.08E−03 −1.34 2.5 hsa-asg-33837.09 6.59 6.83 0.21 100 1.56E−02 −1.33 2.5 hsa-asg-13258 6.41 7.00 7.330.80 100 4.76E−02 −1.29 2.5 hsa-asg-11877 4.12 4.29 4.10 0.22 0 6.37E−03−1.28 2.4 hsa-asg-14048 3.59 4.51 4.18 0.46 0 2.02E−03 −1.28 2.4hsa-asg-14233 8.59 7.53 8.30 0.56 100 7.84E−03 −1.27 2.4 hsa-asg-38845.24 4.08 4.18 0.75 0 3.24E−02 −1.26 2.4 hsa-miR-382 5.08 6.41 5.94 0.5925 4.10E−02 −1.24 2.4 hsa-asg-6788 5.96 6.55 5.94 0.50 25 4.53E−02 −1.242.4 hsa-asg-14237 6.30 5.07 5.86 0.57 75 1.83E−02 −1.22 2.3hsa-asg-14177 2.60 3.27 2.79 0.32 0 3.33E−02 −1.22 2.3 hsa-miR-134 4.424.22 4.49 0.23 0 9.10E−03 −1.21 2.3 hsa-asg-14137 5.82 6.05 5.50 0.62 02.21E−02 −1.19 2.3 hsa-asg-4223 7.69 6.01 7.18 0.85 50 4.00E−02 −1.192.3 hsa-asg-6341 8.71 8.32 8.51 0.33 25 5.56E−03 −1.19 2.3 hsa-asg-132054.92 4.70 5.15 0.46 0 4.23E−02 −1.18 2.3 hsa-asg-9729 5.10 4.92 5.490.56 0 2.36E−02 −1.17 2.2 hsa-asg-13278 8.90 8.17 8.79 0.49 50 5.57E−03−1.16 2.2 hsa-asg-10199 5.18 5.49 5.34 0.13 0 8.53E−03 −1.16 2.2hsa-asg-9126 4.37 5.02 4.58 0.30 0 1.29E−02 −1.16 2.2 hsa-asg-7568 6.145.22 5.56 0.63 25 1.34E−02 −1.16 2.2 hsa-asg-13841 6.93 4.98 5.87 0.8725 4.10E−02 −1.15 2.2 hsa-asg-11285 3.98 3.67 4.05 0.34 0 4.15E−02 −1.122.2 hsa-asg-4339 5.64 5.22 5.49 0.40 0 1.45E−02 −1.11 2.2 hsa-asg-121464.34 5.33 4.80 0.46 0 6.12E−03 −1.07 2.1 hsa-asg-9146 8.05 7.91 7.910.26 0 4.67E−03 −1.06 2.1 hsa-asg-12376 5.41 5.33 4.99 0.48 0 3.33E−02−1.06 2.1 hsa-asg-542 7.32 7.66 7.38 0.36 100 2.06E−02 −1.06 2.1hsa-asg-8003 5.95 5.72 5.76 0.29 0 1.56E−03 −1.06 2.1 hsa-asg-9227 6.236.17 6.27 0.14 75 3.59E−02 −1.04 2.1 hsa-cand720 5.35 4.48 5.11 0.60 02.64E−02 −1.03 2.0 hsa-asg-2858 5.67 6.69 5.93 0.63 25 2.46E−02 −1.022.0 hsa-miR-93 9.68 9.94 10.2 0.46 100 5.74E−03 −1.02 2.0 hsa-asg-36316.60 7.23 6.83 0.29 0 3.82E−03 −1.02 2.0 SD, standard deviation; %,percentage of samples expressing miRNA above background levels; Log2Diff, difference in VSN-transformed expression between LNpos and LNnegsamples.

Example 4 Common miRNAs Differentially Expressed Between Normal CervixSamples And Cancer-negative Inguinal Lymph Nodes and BetweenCancer-positive and Cancer-negative Inguinal Lymph Nodes

The inventors determined the common miRNAs that are differentiallyexpressed between normal cervix (NCX) samples and cancer-negativeinguinal lymph nodes (LNneg) and between cancer-positive (LNpos) andcancer-negative (LNneg) inguinal lymph nodes. For this analysis,differentially expressed miRNAs were identified as having a p-value<0.05 and fold-change>2. These miRNAs represent particularly usefulbiomarkers for monitoring cervical cancer metastasis to inguinal lymphnodes.

The inventors found a total of 71 miRNAs to be differentially expressedbetween both NCX and LNneg samples and between LNpos and LNneg sample(Table 6.). Among these, the average expression levels of 26 miRNAs wereat least 5-fold lower in LNneg samples than their average expressionlevels in either LNpos samples or NCX samples, corresponding to 18verified miRNAs (hsa-miR-182, -183, -189, -200a, -200a*, -200b, -200c,-203, -205, -224, -368, -429, -452, -493-5p, -495, -605, -9, and -9*)and 8 predicted miRNAs (hsa-asg-8174_st1, -7637_st2, -14176_st1,-14153_st2, -1296_st1, -12227_st1, -11715_st2, and -10131_st2).

TABLE 6 miRNAs differentially expressed between both normal cervixsamples and cancer-negative inguinal lymph nodes and betweencancer-positive and cancer-negative inguinal lymph nodes. Fold ChangeFold Change miR Name NCX vs LNneg LNpos vs LNneg hsa-miR-205 413.81332.5 hsa-miR-368 187.2 6.4 hsa-miR-203 171.5 290.1 hsa-miR-495 138.26.7 hsa-asg-5021 97.3 4.4 hsa-miR-200a 80.6 108.1 hsa-miR-299-5p 78.32.8 hsa-miR-493-5p 76.2 8.4 hsa-asg-13254 72.2 3.5 hsa-miR-200b 64.3111.6 hsa-miR-200c 53.7 55.3 hsa-miR-127 50.0 4.0 hsa-miR-432 49.9 3.8hsa-asg-14153 42.2 79.2 hsa-asg-14176 32.1 7.6 hsa-miR-199b 26.1 4.3hsa-miR-134 25.3 2.3 hsa-miR-376a 24.9 3.1 hsa-asg-7637 24.3 48.9hsa-miR-189 24.1 5.5 hsa-miR-429 23.7 63.7 hsa-miR-494 20.8 3.8hsa-miR-224 17.7 24.3 hsa-miR-382 17.4 2.4 hsa-miR-9* 14.9 6.7 hsa-miR-912.4 8.5 hsa-miR-452 12.2 6.2 hsa-miR-605 10.6 11.7 hsa-asg-10131 8.38.1 hsa-miR-27b 8.2 4.3 hsa-miR-200a* 7.6 18.5 hsa-miR-183 7.6 14.8hsa-miR154 7.4 3.7 hsa-asg-1296 7.3 8.2 hsa-miR-182 7.1 10.3hsa-asg-7471 6.9 4.8 hsa-asg-13205 6.6 2.3 hsa-asg-12227 6.4 13.2hsa-asg-11715 5.5 9.9 hsa-asg-924 5.3 4.3 hsa-asg-14233 5.3 2.4hsa-miR-27a 5.3 2.8 hsa-asg-8174 5.2 7.5 hsa-asg-14146 5.0 3.1hsa-asg-1596 4.7 5.4 hsa-asg-9980 4.2 4.4 hsa-asg-9198 4.2 6.3hsa-asg-12139 4.2 5.0 hsa-asg-13705 4.1 3.8 hsa-miR-576 4.1 6.6hsa-asg-11361 4.0 4.6 hsa-miR-34b 4.0 3.0 hsa-asg-13258 3.9 2.5hsa-asg-13997 3.8 2.3 hsa-asg-10247 3.8 4.3 hsa-asg-8428 3.5 5.8hsa-asg-2967 3.4 3.4 hsa-miR-422a 3.4 4.8 hsa-asg-548 3.2 4.2hsa-miR-561 3.2 4.2 hsa-miR-422b 3.2 4.1 hsa-miR-141 3.2 19.7hsa-cand720 3.1 2.0 hsa-asg-3998 2.7 4.3 hsa-asg-11877 2.7 2.4hsa-asg-9723 2.6 3.1 hsa-asg-14048 2.6 2.4 hsa-miR-31 2.6 2.6hsa-asg-11285 2.5 2.2 hsa-asg-8266 2.4 2.9 hsa-asg-1399 2.3 2.6

Example 5 Ort-PCR Verification of Differentially Expressed Micro-RNAS inCancer-positive and Cancer-Negative Lymph Nodes from Cervical CancerPatients

The inventors also evaluated the differential expression of selectedmiRNAs in LNpos and LNneg samples (described in Example 1, Table 3) byqRT-PCR. qRT-PCR reactions were performed using TaqMan® MicroRNA Assays(Applied Biosystems; Foster City, Calif., USA) specific for eleven ofthe most differentially expressed miRNAs between LNpos and LNneg samplesand between NCX vs LNneg samples (hsa-miR-205, -203, -429, -200a, -200c,-141, -183, -182, -189, -200b and -224). Assays were performed accordingto the manufacturer's instructions with 15 ng of input total RNA fromone of eight samples (LNpos 1-4, LNneg 1-4) and were incubated in a7900HT Fast Real-Time PCR system (Applied Biosystems, Foster City,Calif., USA). Initial data analysis was done using the 7500 Fast SystemSDS V2.3 software (Applied Biosystems). miR-16, whose expression leveldid not change between the different samples, was used fornormalization.

Results of the qRT-PCR assays revealed that differences in expressionlevels of the 11 miRNAs, between LNpos and LNneg samples were conservedand were very similar to those observed by microarray analysis (FIGS.1A-1B).

These data demonstrate that both qRT-PCR and microarray analysis areeffective methods for quantifying miRNA expression when evaluatingmetastasis of cervical cancer to inguinal lymph nodes.

Example 6 ORT-PCR Verification of Differentially Expressed Micro-RNAS byAnalysis of Additional Cancer-positive and Cancer-negative Lymph Nodesfrom Cervical Cancer Patients

A second set of FFPE tissue samples, distinct from those described inTable 3 above, was purchased from ProteoGenex. Samples were preparedfrom eight cancer-positive (LNpos5-12) and four cancer-negative(LNneg5-8) lymph nodes that were obtained from twelve different patientswith cervical squamous cell carcinoma (cervical cancer) (Table 7). Apathology report and hematoxilin-eosin (H&E)-stained slide wereavailable for each sample. H&E-stained slides were reviewed by apathologist at Asuragen to confirm lymph node statuses as cancercell-positive or cancer cell-negative.

TABLE 7 Patient and tissue sample pathology information for lymph nodesfrom cervical cancer patients. Lymph nodes from four patients werecancer cell-negative (LNneg), and lymph nodes from eight patients werecancer cell-positive (LNpos). TNM Stage AJCC Patient (Sobin and StageSample Age (yr) Cancer Cervix Tumor Wittekind, (Greene, Lymph Node IDEthnicity Diagnosis Histological Diagnosis 2002) 2002) Metastases LNneg524 normal squamous cell carcinoma T1a2N0M0 IA2 0/4 Caucasian moderatelydifferentiated LNneg6 44 normal squamous cell carcinoma T1b1N0M0 IB1 0/10 Caucasian moderately differentiated LNneg7 44 normal squamous cellcarcinoma T1b1N0M0 IB1 0/6 Caucasian moderately differentiated LNneg5 58normal squamous cell carcinoma T1b1N0M0 IB1 0/8 Caucasian poorlydifferentiated LNpos5 31 metastatic squamous cell carcinoma T1b1N1M0IIIB 2/5 Caucasian moderately differentiated LNpos6 40 metastaticsquamous cell carcinoma T1b1N1M0 IIIB 1/5 Caucasian moderatelydifferentiated LNpos7 21 metastatic squamous cell carcinoma T1b1N1M0IIIB 2/9 Caucasian poorly differentiated LNpos8 56 metastatic squamouscell carcinoma T1b1N1M0 IIIB 3/3 Caucasian moderately differentiatedLNpos9 72 metastatic squamous cell carcinoma T1b1N1M0 IIIB 2/8 Caucasianpoorly differentiated LNpos10 25 metastatic squamous cell carcinomaT2bN1M1 IVB 2/7 & ovary Caucasian moderately differentiated LNpos11 32metastatic squamous cell carcinoma T1b1N1M0 IIIB  3/10 Caucasian poorlydifferentiated LNpos12 58 metastatic squamous cell carcinoma T2bN1M0IIIB 2/4 Caucasian moderately differentiated SCC, squamous cellcarcinoma.

The differential expression of selected miRNAs was evaluated inLNpos5-12 and samples by qRT-PCR. qRT-PCR reactions were performed usingTaqMan® MicroRNA Assays (Applied Biosystems) specific for eleven miRNAs(hsa-miR-205, -203, -429, -200a, -200c, -141, -183, -182, -189, -200b,and -224) that are among those most differentially expressed betweenLNpos1-4 and LNneg1-4 samples and between NCX vs LNneg1-4 samples (Table6). Assays were performed with 15 ng of input total RNA from one oftwelve samples (LNpos 5-12, LNneg 5-8), and data were analyzed asdescribed above in Example 5.

Differential expression was confirmed for seven of the eleven miRNAstested (FIG. 2B). The seven miRNAs (miR-205, -203, -429, -200b, -200a,-200c, and -141) were expressed at levels that were at least 100-foldlower in the cancer-negative lymph node samples (LNneg5-8) than in thecancer-positive lymph node samples (LNpos5-12) and correspond to thesame miRNAs that were identified as most differentially-expressed byqRT-PCR between lymph node samples LNpos1-4 and LNneg1-4 (FIG. 2A, FIG.2D). Differential expression of four of the miRNAs was not observed byqRT-PCR quantification with this set of lymph node samples (LNpos5-12and LNneg5-8). These four miRNAs were the least differentially-expressedbetween lymph nodes LNpos1-4 and LNneg1-4, with fold changes varyingbetween 10- and 35-fold (FIG. 2A, FIG. 2D).

Quantification of these miRNAs by qRT-PCR in an independent set of lymphnodes from cervical cancer patients validates their differentialexpression in cancer-positive and cancer-negative lymph nodes. The sevenmiRNAs (miR-205, -203, -429, -200b, -200a, -200c, and -141) representuseful markers for the identification of cervical tumor cells in lymphnodes of cervical cancer patients (FIG. 2C).

Example 7 Identification of Classifiers for Cancer-positive Lymph Nodesin Patients with Metastatic Cervical Carcinoma

The inventors have demonstrated that miRNA quantification and expressionanalysis can differentiate cancer-positive and cancer-negative lymphnodes from the inguinal region of cervical cancer patients. To furtherdemonstrate the utility of specific miRNA markers as diagnosticanalytes, the inventors quantified specific combinations of miRNAs byqRT-PCR to determine if any such combinations enhanced the distinctionbetween cancer-positive and cancer-negative lymph nodes from cancerpatients. qRT-PCR assays were performed as described in Example 5 above.For miRNA combinations quantified by qRT-PCR, raw Ct values from thequantification of individual miRNAs were added then the Ct value ofmiR-16 was subtracted. For miRNA combinations quantified by microarrayexpression analysis, ΔH values for each individual miRNA were added. Theinventors determined that quantification of several miRNA combinations(miR-205+miR-429; miR-205+miR-203; miR-429+miR-203;miR-425+miR-203+miR-205) enhances the distinction between cancerpositive and cancer-negative lymph nodes from cervical cancer patients(FIG. 3).

These combinations serve as non-limiting examples. Combinations ofqRT-PCR or microarray data for other microRNAs will serve to enhance thedistinction between cancer-positive and cancer-negative lymph nodes.Combinations may include subtraction of average Ct values. In that case,if an even number of miRNAs is present in the combination, thesubtraction of Ct values in a combination eliminates the need tonormalize the data.

Example 8 Detection of RNA from Cervical Cancer Cells in a Background ofCancer-negative Lymph Node RNA

Routine, histological examination of lymph nodes for the presence ofcancer metastasis utilizes light microscopic examination offormalin-fixed, paraffin-embedded (FFPE) lymph node tissue that has beenstained with hematoxylin and eosin (H&E). The sensitivity of thistechnique for detecting cancer is approximately 1 cancer cell per 100normal cells.

First, the inventors evaluated the sensitivity of detection of cervicalcancer miRNA markers using qRT-PCR. Sensitivities of detection weredetermined for two synthetic miRNAs (hsa-miR-203 and hsa-miR-205) thatwere shown by microarray analysis to be overexpressed by ≧1,000 fold(>10 ΔCt) in cancer-positive lymph nodes (LNpos) when compared withtheir expression in cancer-negative lymph nodes (LNneg). SyntheticmiRNAs (0-10⁸ copies) were mixed with 15 ng of total RNA from acancer-negative lymph node, and qRT-PCR reactions specific for eachmiRNA were performed using TaqMan® MicroRNA Assays (Applied Biosystems).The results of these analyses (FIG. 4) indicate that the limit ofdetection of miR-205 and miR-203 by qRT-PCR in this assay of theinvention is ˜1 cancer cell per ˜10,000 cancer-negative lymph nodecells.

Next, the inventors determined the sensitivities of detection forhsa-miR-205, -429, -200a, -200c, and -141 following the serial 10-folddilution (1:10; 1:100; 1:1,000; 1:10,000, 1:100,000, and 1:1,000,000) oftotal RNA from cervical cancer cells (TUM) into a background of totalRNA (15 ng) from a cancer-negative lymph node (LNneg). qRT-PCR reactionsspecific for the each indicated miRNA were performed using TaqMan®MicroRNA Assays (Applied Biosystems). A ΔCt of at least 3.3 (10-fold)from that for the LNneg sample was required for a difference in Ctvalues to be considered statistically significant. Results of theseanalyses (FIG. 5A) indicate that the sensitivity of detection was the1:100 dilution for miR-205, -429, and -200a and the 1:10 dilution formiR-200c and -141.

Finally, the inventors determined the sensitivities of detection forhsa-miR-205, -429, -200a, -200c, and -141 following the serial 10-folddilution (1:10; 1:100; 1:1,000; 1:10,000, 1:100,000) of total RNA from acancer-positive lymph node, containing ˜70% cancerous cells, into abackground of total RNA (15 ng) from a cancer-negative lymph node. (FIG.5B). Results of these analyses indicate that the sensitivity ofdetection was the 1:100 dilution for mir-205 and miR-429 and the 1:10dilution for miR-200a, -200c, and -141.

Example 9 Melanoma Samples and RNA Isolation

Three melanoma (MEL1-3) (Table 8), four cancer-positive lymph node(LNpos1A and LNpos2B-4B), and seven cancer-negative lymph node(LNneg1-7) (Table 9) frozen tissue samples were purchased fromProteoGenex (ProteoGenex, Inc.; Culver City, Calif., USA). Melanomasamples were from the chest or shoulder regions of patients, and ten ofeleven lymph nodes were axillary, to match the origin of the tumors. Allthe samples were from different patients. Total RNA isolation from themelanoma and lymph node tissue samples was performed using the mirVana™miRNA Isolation Kit (Ambion; cat. no. AM1560), following themanufacturer's protocol. Purified total RNA from all samples wasquantified using a Nanodrop® ND-1000 (Nanoprop Technologies).

An independent set of three melanoma (MEL4-6) and four cancer-positivelymph node (LNpos5-8) formalin-fixed, paraffin-embedded (FFPE) tissuesamples were purchased from ProteoGenex (ProteoGenex, Inc.; Culver City,Calif., USA). Total RNA isolation from these samples was performed usingthe RecoverAll™ Total Nucleic Isolation Kit (cat. No. AM1975; Ambion,Inc.; Austin, Tex., USA) as described above in Example 1. Purified totalRNA was quantified using a Nanodrop® ND-1000 (Nanodrop Technologies;Wilmington, Del., USA). A hematoxilin-eosin (H&E)-stained slide fromeach sample was reviewed by a pathologist at Asuragen who confirmed thepresence of cancer cells in the lymph nodes.

TABLE 8 Patient and tissue pathology information for melanoma tissuesamples. TNM Stage (Sobin and Wittekind, 2002); Clark's Level (Clark etal., 1969); Breslow Micro-staging (Breslow, 1970). MEL1-3 were providedas frozen biopsies. MEL4-6 were provided as FFPE samples. Patient Age(yr) Clinical Breslow Sex Histological TNM Stage LN Clark's Micro-Sample Ethnicity Diagnosis Stage Group Mets Level Staging MEL1 70pigmented T3aN0M0 IIA No IV 4 mm Male melanoma, epithelia Caucasian celltype, left shoulder MEL2 47 pigmented T4bN3M0 III 7/9 V 20 mm  Malemelanoma, Caucasian epithelioid cell type, anterior chest wall MEL3 48poorly pigmented T4bN2M0 III 2/9 II 8 mm Female melanoma, spindleCaucasian cell type, scapula MEL4 17 melanoma, T4aN0M0 IIB No V 6 mmFemale epithelioid cell type Caucasian MEL5 37 poorly pigmented T4bN1M0IIB 1/9 V 11 mm  Female melanoma, Caucasian epithelioid cell type,shoulder MEL6 67 poorly pigmented, T4bN0M0 IIC No IV 7 mm Femalemalignant nodular Caucasian carcinoma,, epithelioid type, shoulder LNMets, number of lymph nodes with cancer metastasis/total number of lymphnodes taken.

TABLE 9 Patient and tissue pathology information for lymph node samples(cancer-negative and cancer-positive) from melanoma cancer patients.LNpos1A, LNpos2-4B, and LNneg1-7 samples were provided as frozenbiopsies. LNpos5-8 samples were provided as FFPE samples. TNM Stage(Sobin and Wittekind, 2002); Clark's Level (Clark et al., 1969); BreslowMicro-staging (Breslow, 1970). Patient Sample # Age (yr) Melanoma GroupSex Cancer Histological TNM Clinical Stage Breslow Micro- Node EthnicityDiagnosis Diagnosis Stage Group LN Mets Clark's Level Staging LNneg1 66Normal melanoma, T4bN0M0 IIB 0/7 III 20 mm Axillary Male epithelioid &spindle Caucasian cell type LNneg2 71 Normal pigmented melanoma, T4bN0M0IIB 0/3 IV  7 mm Axillary Male epithelioid cell type Caucasian LNneg3 50Normal non-pigmented T4bN0M0 IIB 0/8 III-IV  5 mm Axillary Femalemelanoma, spindle cell Caucasian type LNneg4 67 Normal melanoma,epithelioid T4bN2M0 III  6/10 NA NA Inguinal Female & spindle cell typeCaucasian LNneg5 47 Normal poorly pigmented T4bN2M0 III 2/6 III 15 mmAxillary Male melanoma, epithelioid Caucasian cell type LNneg6 35 Normalmelanoma, epithelioid T4bN2M0 III 2/6 V 45 mm Axillary Male & spindlecell type Caucasian LNneg7 64 Normal pigmented melanoma, T4bN2M0 III 2/7NA NA Axillary Female epithelioid cell type Caucasian LNpos1A 59Melanoma melanoma, epithelioid T4bN2M0 III 7/8 V 30 mm Axillary Femalecell type Caucasian LNpos2B 80 Melanoma pigmented melanoma, NA CUP  2/10NA NA Axillary Female spindle cell type Caucasian LNpos3B 56 Melanomapigmented melanoma, NA recurrent 3/5 NA NA Axillary Male epithelioid &spindle Caucasian cell type LNpos4B 68 Melanoma pigmented melanoma, NArecurrent  3/16 NA NA Axillary Female epithelioid cell type CaucasianLNpos5 75 Melanoma poorly pigmented T4bN2M0 IIIB  2/15 V 22 mm Femalemelanoma, epithelioid Caucasian cell type LNpos6 47 Melanoma pigmentedmelanoma, T4bN2M0 IIIB 7/9 V 20 mm Female epithelioid cell typeCaucasian LNpos7 67 Melanoma poorly pigmented T4bN2M0 IIC No IV  7 mmFemale melanoma, epithelioid Caucasian cell type LNpos8 62 Melanomamelanoma, T4bNIM0 III 1/9 N/A N/A Female epithelioid cell type Caucasian(regressed) CUP, cancer of unknown primary origin; recurrent, recurrenceof melanoma; NA, not available; LN Mets, number of lymph nodes withcancer metastasis/total number of lymph nodes taken.

Example 10 Identification of miRNAs Differentially Expressed BetweenMelanoma Samples and Cancer-negative Lymph Node Samples

To identify miRNAs that represent useful markers for metastasis ofmelanoma cancers to lymph nodes, the inventors identified miRNAs thatare expressed at significantly higher levels in melanoma samples frompatients with melanoma than in cancer-negative lymph nodes from patientswith melanoma.

miRNA expression was determined for two melanoma samples (MEL1, MEL3,described in Example 9 and Table 8 above) and seven cancer-negativelymph nodes (LNneg1-7, described in Example 9 and Table 9 above). Foreach tissue sample, microRNA-containing fractions were purified from 10μg of total RNA using a flashPAGE™ Fractionation Apparatus (Ambion) andanalyzed with microarrays as described above in Example 2.

The inventors identified 90 human miRNAs (34 verified and 56 predicted)with average expression levels that were significantly higher in the twomelanoma (MEL) samples than in cancer-negative lymph nodes (LNneg) (log2Diff(MEL vs LNneg)≧1 and p-value<0.05) (Table 10). Among the verifiedmiRNAs, the average expression level of hsa-miR-205 was 260-fold higherin MEL samples than in LNneg; the average expression levels of fourmiRNAs (hsa-miR-203, -211, -509, and -513) were between 60- and 160-foldhigher in MEL samples than in LNneg samples; four miRNAs (hsa-miR-200c,-132, -506, and -508) had average expression levels between 10- and15-fold higher in MEL than in LNneg samples, and 11 miRNAs had averageexpression levels between 5- and 10-fold higher in MEL samples (Table10). In addition, 19 miRNAs had average expression levels that werelower in MEL samples than in LNneg samples. Among these, one (hsa-miR-1)was expressed at a level at least 5-fold lower in MEL samples (Table10). Among the predicted miRNAs, one (hsa-asg-5638-st2) was expressed ata level more than 100-fold higher in MEL samples compared to LNnegsamples, and seven miRNAs (hsa-asg-11338_st2, -5630_st2, -13997_st2,-8428_st2, -9186_st1, -1296_st1, and -9723_st2) had average expressionlevels between 5- and 25-fold higher in MEL samples (Table 10).

TABLE 10 MicroRNAs Differentially Expressed Between Melanoma Samples(MEL) and Cancer-Negative Lymph Node Samples (LNneg) from MelanomaPatients. Micro Probe MEL LNneg RNA ID 1 3 Mean SD % 1 2 3 hsa-miR-hsa-miR- 13.32 11.35 12.33 1.40 100 5.03 3.70 4.67 205 205_st1 hsa-miR-hsa-miR- 13.82 12.43 13.13 0.98 100 4.37 5.90 5.84 203 203_st2 hsa-miR-hsa-miR- 10.72 10.18 10.45 0.38 100 3.20 5.75 2.62 211 211_st1 hsa-miR-hsa-miR- 11.61 13.17 12.39 1.11 100 6.09 8.04 6.00 509 509_st2 hsa-miR-hsa-miR- 10.22 12.33 11.28 1.49 100 3.01 7.12 5.52 513 513_st1 hsa-miR-hsa-miR- 12.67 10.60 11.64 1.47 100 8.90 7.29 7.04 200c 200c_st1hsa-miR- hsa-miR- 9.33 9.05 9.19 0.20 100 6.62 4.36 4.55 132 132_st1hsa-miR- hsa-miR- 8.90 11.22 10.06 1.64 100 6.15 7.11 6.59 506 506_st2hsa-miR- hsa-miR- 9.31 10.88 10.09 1.11 100 6.06 7.31 7.14 508 508_st1hsa-miR- hsa-miR- 8.58 10.58 9.58 1.41 100 5.47 6.35 6.90 183 183_st1hsa-miR- hsa-miR- 11.29 8.88 10.08 1.71 100 6.34 6.10 6.86 200b 200b_st1hsa-miR- hsa-miR- 6.95 9.35 8.15 1.69 100 4.62 5.83 5.85 510 510_st2hsa-miR- hsa-miR- 7.37 4.43 5.90 2.08 50 2.59 2.44 3.59 561 561_st1hsa-miR- hsa-miR- 7.49 7.26 7.38 0.16 100 3.64 3.70 3.26 493-5p493-5p_st1 hsa-miR- hsa-miR- 5.44 6.62 6.03 0.83 50 3.01 3.06 2.89 514514_st1 hsa-miR- hsa-miR- 8.59 8.32 8.45 0.19 100 4.80 5.52 5.44 584584_st2 hsa-miR-9 hsa-miR- 5.84 8.06 6.95 1.57 100 3.64 4.12 3.59 9_st2hsa-miR- hsa-miR- 6.71 4.23 5.47 1.76 50 2.80 2.44 3.26 576 576_st2hsa-miR-9* hsa-miR-9-_st2 6.94 9.80 8.37 2.02 100 5.62 5.46 5.15hsa-miR-96 hsa-miR- 5.62 6.89 6.26 0.89 50 3.64 4.74 2.76 96_st1hsa-miR- hsa-miR- 10.08 11.77 10.92 1.19 100 7.90 8.91 8.60 182 182_st1hsa-miR- hsa-miR- 5.60 4.43 5.01 0.82 0 3.48 3.06 2.95 605 605_st1hsa-miR- hsa-miR- 9.12 9.25 9.19 0.09 100 7.78 6.55 6.63 421 421-3p_st1hsa-miR- hsa-miR- 5.71 6.41 6.06 0.50 50 2.80 4.84 3.74 432 432_st2hsa-miR- hsa-miR- 5.86 3.23 4.55 1.87 0 3.01 3.06 2.62 429 429_st1hsa-miR- hsa-asg- 4.53 4.61 4.57 0.06 0 3.20 2.69 2.76 592 4400_st1hsa-miR- hsa-miR- 6.70 5.64 6.17 0.75 100 4.26 4.84 4.28 495 495_st1hsa-miR- hsa-miR- 5.19 4.69 4.94 0.35 0 2.90 2.82 4.01 187 187_st1hsa-miR- hsa-miR- 7.91 7.64 7.77 0.20 100 6.09 5.99 6.13 382 382_st1hsa-miR- hsa-miR- 8.86 9.76 9.31 0.64 100 7.72 7.95 8.14 34a 34a_st1hsa-miR- hsa-miR- 6.15 5.39 5.77 0.54 50 4.01 4.68 4.61 376a 376a_st2hsa-miR- hsa-miR- 8.98 9.21 9.10 0.16 100 7.31 7.99 8.32 193b 193b_st1hsa-miR- hsa-miR- 11.08 11.50 11.29 0.30 100 10.51 10.21 10.58 222222_st2 hsa-miR- hsa- 5.74 5.98 5.86 0.17 50 5.03 4.84 4.42 365cand708_st1 hsa-miR- hsa-miR- 7.57 6.77 7.17 0.56 100 6.09 6.52 6.84 494494_st1 hsa-miR- hsa-miR- 12.18 11.88 12.03 0.21 100 10.84 11.49 11.3827a 27a_st2 hsa-miR- hsa-miR- 7.04 6.95 6.99 0.07 100 5.93 6.35 6.09 379379_st1 hsa-miR- hsa-miR- 13.13 12.40 12.77 0.52 100 11.98 12.08 12.0527b 27b_st1 hsa-miR-21 hsa-miR- 13.86 13.68 13.77 0.13 100 13.12 13.6513.21 21_st2 hsa-miR-24 hsa-miR- 13.74 13.62 13.68 0.08 100 12.70 13.2613.20 24_st1 hsa-miR- hsa-miR- 13.74 13.62 13.68 0.09 100 13.34 13.1113.25 23b 23b_st1 hsa-miR- hsa-miR- 12.85 13.24 13.04 0.28 100 12.8012.57 12.65 221 221_st1 hsa-let-7e hsa-let- 12.45 12.79 12.62 0.23 10012.00 12.23 12.19 7e_st1 hsa-miR- hsa-miR- 11.91 12.01 11.96 0.07 10011.74 11.66 11.37 30b 30b_st2 hsa-miR- hsa-miR- 5.23 5.32 5.28 0.07 04.92 4.68 5.11 618 618_st2 hsa-miR- hsa-miR- 13.95 13.94 13.95 0.01 10013.63 13.50 13.74 23a 23a_st2 hsa-miR- hsa-miR- 12.59 12.36 12.48 0.17100 12.63 12.97 13.05 199a* 199a-_st1 hsa-miR- hsa-miR- 12.82 12.1412.48 0.48 100 13.04 13.35 13.14 145 145_st1 hsa-miR- hsa- 11.04 10.9010.97 0.10 100 10.96 11.55 11.65 99a cand160_st2 hsa-miR- hsa-miR- 12.6211.48 12.05 0.81 100 12.73 13.02 12.80 195 195_st2 hsa-miR- hsa-asg-6.77 6.19 6.48 0.41 50 7.82 7.13 6.86 331 14205_st1 hsa-miR- hsa-miR-12.03 11.24 11.63 0.56 100 12.03 12.78 12.72 143 143_st2 hsa-miR-hsa-miR- 9.62 8.44 9.03 0.84 100 9.52 10.13 10.16 199a 199a_st2 hsa-miR-hsa-miR- 11.60 10.60 11.10 0.71 100 12.15 12.35 12.36 10b 10b_st2hsa-miR- hsa-miR- 5.54 5.34 5.44 0.14 0 7.07 6.62 6.44 339 339_st1hsa-miR-7 hsa-miR- 8.41 8.58 8.50 0.12 100 10.25 9.66 9.09 7_st1hsa-miR- hsa-miR- 10.46 10.28 10.37 0.13 100 11.90 11.78 11.44 148a148a_st1 hsa-miR- hsa-miR- 9.95 8.90 9.43 0.74 100 11.25 11.01 10.82 29b29b_st1 hsa-miR- hsa-miR- 12.96 11.27 12.12 1.19 100 13.38 13.73 13.84342 342_st1 hsa-miR- hsa- 6.65 5.48 6.06 0.83 0 7.19 7.68 7.66 497miR43_st1 hsa-miR- hsa-miR- 5.19 5.59 5.39 0.28 0 6.29 6.90 6.91 215215_st1 hsa-miR- hsa-miR- 8.33 9.67 9.00 0.95 100 10.24 10.16 10.60 192192_st2 hsa-miR-17- hsa-miR-17- 4.99 5.01 5.00 0.02 0 6.93 6.98 6.53 3p3p_st1 hsa-miR-95 hsa-miR- 8.49 5.91 7.20 1.82 100 8.68 8.53 8.61 95_st1hsa-miR- hsa-miR- 7.55 6.46 7.00 0.77 100 8.78 8.27 8.79 505 505_st1hsa-miR- hsa-miR- 5.57 4.75 5.16 0.58 0 8.05 6.96 6.71 598 598_st2hsa-miR- hsa-miR- 8.52 8.33 8.43 0.13 100 11.18 10.41 10.48 29c 29c_st2hsa-miR- hsa-miR- 3.97 2.35 3.16 1.15 0 3.72 5.36 4.83 133b 133b_st2hsa-miR- hsa-miR- 4.70 3.23 3.96 1.04 0 5.14 6.04 6.16 133a 133a_st1hsa-miR-1 hsa-miR- 7.28 5.75 6.51 1.08 100 8.27 9.62 8.72 1_st1 hsa-asg-hsa-asg- 9.47 11.07 10.27 1.13 100 3.20 5.22 3.02 5638 5638_st2 hsa-asg-hsa-asg- 7.15 9.46 8.30 1.63 100 4.32 3.88 3.54 11338 11338_st2 hsa-asg-hsa-asg- 6.83 9.45 8.14 1.85 100 6.46 4.19 3.02 5630 5630_st2 hsa-asg-hsa-asg- 6.97 4.49 5.73 1.75 50 3.01 2.57 3.49 13997 13997_st2 hsa-asg-hsa-asg- 6.75 4.72 5.74 1.44 100 3.01 3.06 2.76 8428 8428_st2 hsa-asg-hsa-asg- 7.60 8.05 7.82 0.32 100 4.26 6.07 5.35 9186 9186_st1 hsa-asg-hsa-asg- 6.52 4.64 5.58 1.33 50 3.20 2.94 2.62 1296 1296_st1 hsa-asg-hsa-asg- 5.74 5.05 5.39 0.48 100 3.01 3.29 2.36 9723 9723_st2 hsa-asg-hsa-asg- 6.09 4.55 5.32 1.09 50 2.80 2.69 3.59 11380 11380_st2 hsa-asg-hsa-asg- 4.47 6.30 5.39 1.30 50 3.11 3.40 3.08 2261 2261_st1 hsa-asg-hsa-asg- 8.07 9.09 8.58 0.72 100 5.74 6.92 7.20 1519 1519_st1 hsa-asg-hsa-asg- 5.91 4.43 5.17 1.05 50 3.11 3.06 4.13 1794 1794_st1 hsa-asg-hsa-asg- 6.25 6.91 6.58 0.47 50 4.48 4.51 4.49 12271 12271_st1 hsa-asg-hsa-asg- 5.44 7.37 6.41 1.37 50 3.80 3.70 4.39 14208 14208_st1 hsa-asg-hsa-asg- 4.53 6.90 5.71 1.68 50 3.72 3.70 3.14 8486 8486_st2 hsa-asg-hsa-asg- 10.46 10.75 10.61 0.20 100 8.43 8.50 9.42 5640 5640_st1hsa-cand317 hsa- 6.62 4.61 5.61 1.42 50 3.48 4.19 2.76 cand317_st1hsa-asg- hsa-asg- 4.59 5.46 5.02 0.62 50 3.48 3.88 3.02 2335 2335_st1hsa-asg- hsa-asg- 5.79 3.55 4.67 1.58 50 3.30 3.29 3.02 1587 1587_st1hsa-asg- hsa-asg- 5.62 6.32 5.97 0.49 50 4.26 3.83 4.83 4803 4803_st2hsa-asg- hsa-asg- 10.94 10.80 10.87 0.10 100 9.51 8.48 9.66 1327813278_st2 hsa-asg-497 hsa-asg- 7.46 7.19 7.33 0.19 100 5.71 5.67 5.80497_st2 hsa-asg- hsa-asg- 5.37 6.00 5.69 0.44 0 3.11 4.22 5.02 97969796_st1 hsa-asg- hsa-asg- 6.39 4.55 5.47 1.30 50 4.58 3.29 3.93 1003110031_st1 hsa-asg- hsa-asg- 6.00 5.55 5.78 0.32 50 4.37 4.33 3.74 99809980_st1 hsa-asg- hsa-asg- 4.94 6.89 5.91 1.37 50 4.32 4.26 4.46 1245312453_st2 hsa-asg- hsa-asg- 7.71 5.70 6.70 1.42 50 5.07 4.79 5.45 1148911489_st1 hsa-asg- hsa-asg- 4.90 4.72 4.81 0.12 0 2.90 3.18 3.38 1136111361_st2 hsa-asg- hsa-asg- 7.18 6.67 6.93 0.36 100 5.14 5.02 5.40 1280712807_st2 hsa-asg- hsa-asg- 6.44 6.14 6.29 0.22 100 4.71 4.57 4.83 75117511_st2 hsa-asg- hsa-asg- 6.93 5.21 6.07 1.21 50 4.84 4.88 5.07 97629762_st1 hsa-asg- hsa-asg- 7.39 8.00 7.69 0.43 50 5.62 6.44 6.84 1237212372_st2 hsa-asg- hsa-asg- 9.22 8.68 8.95 0.38 100 8.20 6.66 8.11 42234223_st2 hsa-asg- hsa-asg- 7.02 6.29 6.65 0.52 100 5.98 5.56 5.61 1140211402_st1 hsa-asg- hsa-asg- 7.79 7.46 7.62 0.23 100 6.52 6.33 6.46 86338633_st1 hsa-asg- hsa-asg- 7.21 6.62 6.91 0.42 100 5.23 6.18 5.94 47804780_st1 hsa-asg- hsa-asg- 6.98 6.99 6.98 0.01 100 5.47 5.84 5.79 91179117_st1 hsa-asg- hsa-asg- 6.19 5.29 5.74 0.64 50 4.84 4.04 4.21 13621362_st2 hsa-asg- hsa-asg- 10.75 10.40 10.57 0.25 100 8.77 9.78 9.0914233 14233_st1 hsa-asg- hsa-asg- 13.14 12.47 12.80 0.47 100 12.38 11.4111.96 13309 13309_st2 hsa-asg- hsa-asg- 8.42 7.09 7.76 0.94 100 6.866.57 6.69 13841 13841_st2 hsa-asg- hsa-asg- 7.42 7.26 7.34 0.11 100 5.786.08 6.22 14001 14001_st1 hsa-asg- hsa-asg- 5.65 5.64 5.65 0.01 50 4.585.14 4.80 12320 12320_st2 hsa-asg- hsa-asg- 8.14 7.07 7.61 0.76 100 6.356.73 6.63 9227 9227_st1 hsa-asg- hsa-asg- 7.30 7.00 7.15 0.21 100 5.976.18 6.36 7922 7922_st2 hsa-asg- hsa-asg- 9.19 9.35 9.27 0.11 100 7.768.44 8.32 9261 9261_st1 hsa-cand732 hsa- 7.47 8.16 7.82 0.49 100 6.446.34 6.99 cand732_st2 hsa-asg- hsa-asg- 6.05 6.04 6.05 0.00 50 5.30 5.064.72 13924 13924_st1 hsa-asg- hsa-asg- 5.84 5.46 5.65 0.27 0 4.14 4.974.83 2889 2889_st2 hsa-asg- hsa-asg- 6.79 7.43 7.11 0.45 100 5.74 5.906.09 12381 12381_st1 hsa-asg- hsa-asg- 7.36 7.75 7.56 0.28 100 6.53 6.646.31 8003 8003_st2 hsa-asg- hsa-asg- 6.51 5.64 6.08 0.61 100 4.80 5.184.64 7262 7262_st1 hsa-asg- hsa-asg- 10.31 11.71 11.01 0.99 100 9.659.98 10.04 11850 11850_st1 hsa-asg- hsa-asg- 6.60 6.87 6.74 0.19 1005.93 5.87 5.70 7027 7027_st2 hsa-asg- hsa-asg- 8.58 9.10 8.84 0.37 1007.89 7.38 8.02 12937 12937_st2 hsa-asg-345 hsa-asg- 5.60 5.75 5.68 0.1150 4.84 4.74 5.02 345_st1 hsa-asg- hsa-asg- 6.63 6.13 6.38 0.35 100 6.345.26 5.38 4969 4969_st2 hsa-asg- hsa-asg- 6.92 5.88 6.40 0.73 50 5.175.64 5.64 3798 3798_st1 hsa-asg- hsa-asg- 6.89 7.41 7.15 0.37 100 5.916.08 5.97 4703 4703_st2 hsa-asg- hsa-asg- 4.59 3.55 4.07 0.73 0 3.482.19 2.95 8792 8792_st1 hsa-asg- hsa-asg- 5.89 6.10 5.99 0.15 50 4.585.18 5.61 4460 4460_st2 hsa-asg- hsa-asg- 7.83 8.30 8.06 0.33 100 6.577.31 7.25 4026 4026_st1 hsa-cand421 hsa- 11.03 12.08 11.56 0.75 10010.27 10.91 10.45 cand421_st1 hsa-asg- hsa-asg- 8.43 8.61 8.52 0.13 1006.97 7.90 7.97 3670 3670_st1 hsa-asg- hsa-asg- 7.83 8.38 8.10 0.39 1007.00 7.33 7.12 9130 9130_st2 hsa-asg- hsa-asg- 5.65 6.97 6.31 0.93 505.57 5.43 5.85 7783 7783_st1 hsa-asg- hsa-asg- 5.79 6.48 6.13 0.49 505.10 5.85 4.98 3944 3944_st1 hsa-asg- hsa-asg- 5.74 5.83 5.78 0.07 504.53 5.14 4.88 13798 13798_st1 hsa-asg- hsa-asg- 8.85 9.09 8.97 0.16 1007.58 7.87 8.40 6870 6870_st1 hsa-asg- hsa-asg- 5.41 6.00 5.70 0.42 504.20 4.93 4.72 2460 2460_st1 hsa-asg- hsa-asg- 4.70 5.86 5.28 0.82 504.32 4.74 4.61 11267 11267_st2 hsa-asg- hsa-asg- 6.48 6.11 6.29 0.26 1005.23 5.43 5.31 3604 3604_st1 hsa-asg- hsa-asg- 6.93 6.77 6.85 0.11 1005.98 5.99 5.73 4296 4296_st1 hsa-asg- hsa-asg- 8.56 9.33 8.95 0.54 1007.81 8.41 8.26 8866 8866_st1 hsa-asg- hsa-asg- 5.86 5.36 5.61 0.36 1004.67 5.06 4.93 12471 12471_st1 hsa-asg- hsa-asg- 8.55 7.89 8.22 0.46 1007.07 7.29 7.77 11515 11515_st2 hsa-asg- hsa-asg- 6.21 6.81 6.51 0.42 505.71 5.61 6.20 7252 7252_st1 hsa-asg- hsa-asg- 6.52 6.52 6.52 0.00 1006.31 5.64 6.14 11458 11458_st2 hsa-asg- hsa-asg- 9.17 9.63 9.40 0.33 508.38 8.93 8.47 13453 13453_st1 hsa-asg- hsa-asg- 9.98 10.07 10.02 0.07100 9.11 9.16 9.23 3722 3722_st1 hsa-asg- hsa-asg- 11.02 12.01 11.520.69 100 10.84 10.88 10.76 3992 3992_st2 hsa-asg- hsa-asg- 6.75 7.247.00 0.35 100 6.54 6.44 6.23 4373 4373_st1 hsa-asg- hsa-asg- 7.74 7.747.74 0.00 100 6.91 7.02 7.01 9480 9480_st1 hsa-asg- hsa-asg- 12.87 13.6013.24 0.52 100 12.55 12.77 12.46 7939 7939_st1 hsa-asg- hsa-asg- 6.976.76 6.86 0.15 100 6.16 6.12 6.47 4448 4448_st1 hsa-asg- hsa-asg- 6.627.06 6.84 0.31 100 6.41 6.12 6.50 1338 1338_st2 hsa-asg- hsa-asg- 8.118.21 8.16 0.07 100 7.33 7.29 7.45 11617 11617_st2 hsa-asg-954 hsa-asg-9.97 9.90 9.93 0.05 100 9.28 9.11 9.34 954_st1 hsa-asg- hsa-asg- 6.496.76 6.63 0.19 100 6.04 6.16 5.64 2381 2381_st2 hsa-asg- hsa-asg- 5.896.04 5.97 0.11 50 5.87 5.02 5.40 2697 2697_st1 hsa-asg- hsa-asg- 6.156.34 6.25 0.13 50 5.60 5.52 5.84 2365 2365_st1 hsa-asg- hsa-asg- 5.375.98 5.68 0.43 50 5.00 5.02 5.09 9795 9795_st2 hsa-asg- hsa-asg- 5.305.75 5.53 0.32 50 4.48 4.88 4.67 12457 12457_st2 hsa-asg- hsa-asg- 6.366.35 6.35 0.01 100 5.52 5.83 5.94 2500 2500_st1 hsa-asg- hsa-asg- 6.947.85 7.40 0.64 100 6.68 6.97 7.02 8882 8882_st1 hsa-asg- hsa-asg- 6.035.83 5.93 0.14 100 5.36 5.10 5.11 5973 5973_st1 hsa-asg- hsa-asg- 6.436.80 6.61 0.26 100 5.93 6.59 5.98 6616 6616_st1 hsa-asg- hsa-asg- 6.676.97 6.82 0.21 100 6.42 6.33 6.32 13294 13294_st2 hsa-asg- hsa-asg- 5.475.49 5.48 0.01 100 5.00 4.93 4.35 9521 9521_st2 hsa-asg- hsa-asg- 8.949.21 9.08 0.19 100 8.48 8.25 8.53 7450 7450_st1 hsa-asg- hsa-asg- 4.905.17 5.04 0.20 50 4.53 4.33 4.42 4030 4030_st2 hsa-asg- hsa-asg- 7.277.69 7.48 0.30 100 6.64 6.89 7.15 12406 12406_st1 hsa-asg- hsa-asg- 6.136.11 6.12 0.02 50 5.67 5.90 5.76 12885 12885_st1 hsa-cand873 hsa- 5.865.93 5.90 0.05 100 5.41 5.29 5.02 cand873_st1 hsa-asg- hsa-asg- 13.5114.05 13.78 0.38 100 13.37 13.40 13.27 14201 14201_st2 hsa-asg- hsa-asg-10.48 11.07 10.78 0.42 100 10.50 10.37 10.00 8568 8568_st1 hsa-asg-hsa-asg- 7.88 8.39 8.13 0.36 100 7.57 7.54 7.68 3239 3239_st2 hsa-asg-hsa-asg- 7.66 7.57 7.62 0.07 100 7.19 7.01 7.12 4984 4984_st1 hsa-asg-hsa-asg- 6.32 6.13 6.23 0.14 100 5.98 5.64 5.80 2093 2093_st1hsa-asg-733 hsa-asg- 6.38 6.41 6.39 0.02 100 5.82 6.28 6.01 733_st1hsa-asg-89 hsa-asg- 6.52 6.58 6.55 0.04 100 6.00 6.26 6.17 89_st2hsa-asg- hsa-asg- 6.19 6.04 6.12 0.11 100 5.78 5.56 5.89 12816 12816_st1hsa-asg- hsa-asg- 5.89 5.79 5.84 0.07 0 5.27 5.70 5.61 11057 11057_st1hsa-asg- hsa-asg- 6.67 6.46 6.57 0.15 100 6.11 6.30 6.17 6305 6305_st1hsa-asg- hsa-asg- 5.86 6.03 5.95 0.12 50 5.95 5.59 5.49 4587 4587_st1hsa-asg- hsa-asg- 6.03 6.13 6.08 0.07 50 5.85 6.02 5.92 2483 2483_st2hsa-asg-758 hsa-asg- 6.25 6.20 6.23 0.04 50 6.51 6.71 6.34 758_st1hsa-asg- hsa-asg- 6.34 6.29 6.31 0.04 100 6.38 6.59 6.82 7410 7410_st1hsa-asg- hsa-asg- 9.87 10.06 9.97 0.14 100 10.37 10.21 10.17 28992899_st2 hsa-asg- hsa-asg- 9.14 8.83 8.99 0.21 50 9.10 9.21 9.44 76287628_st2 hsa-asg- hsa-asg- 10.36 10.48 10.42 0.09 100 10.76 10.52 10.9614034 14034_st1 hsa-asg- hsa-asg- 9.65 9.45 9.55 0.14 100 9.69 9.77 9.898253 8253_st2 hsa-asg- hsa-asg- 4.59 4.55 4.57 0.02 0 4.76 5.18 4.724426 4426_st2 hsa-asg- hsa-asg- 6.59 6.14 6.36 0.32 50 6.52 6.73 6.788655 8655_st1 hsa-asg- hsa-asg- 10.98 11.15 11.07 0.12 100 11.43 11.3011.51 10632 10632_st1 hsa-asg-974 hsa-asg- 6.60 6.60 6.60 0.00 100 6.717.06 7.30 974_st2 hsa-asg- hsa-asg- 6.15 6.02 6.09 0.09 0 6.32 6.46 6.607218 7218_st1 hsa-asg-206 hsa-asg- 9.59 9.13 9.36 0.33 50 9.82 9.68 9.76206_st2 hsa-asg- hsa-asg- 7.44 7.52 7.48 0.06 0 7.64 7.74 8.17 35653565_st2 hsa-cand559 hsa- 6.88 7.08 6.98 0.14 100 7.13 7.31 7.70cand559_st1 hsa-asg- hsa-asg- 8.00 7.96 7.98 0.03 100 8.67 8.09 8.481809 1809_st1 hsa-asg- hsa-asg- 8.37 8.28 8.32 0.07 100 8.63 8.61 8.744020 4020_st2 hsa-asg- hsa-asg- 7.95 7.76 7.86 0.14 100 8.02 8.21 8.4210392 10392_st1 hsa-asg- hsa-asg- 6.07 6.15 6.11 0.06 0 6.32 6.60 6.499501 9501_st1 hsa-asg-976 hsa-asg- 6.27 6.18 6.22 0.06 50 6.29 6.82 6.71976_st2 hsa-asg- hsa-asg- 9.27 9.72 9.49 0.32 100 9.97 9.92 9.87 57495749_st2 hsa-asg- hsa-asg- 8.63 9.06 8.84 0.30 100 9.43 9.12 9.14 86318631_st1 hsa-asg- hsa-asg- 8.92 8.89 8.90 0.02 50 9.32 9.13 9.24 70517051_st1 hsa-asg- hsa-asg- 5.30 5.95 5.63 0.46 50 6.16 5.78 6.06 1050610506_st2 hsa-asg-507 hsa-asg- 2.58 2.61 2.60 0.02 0 3.11 2.82 2.89507_st1 hsa-asg- hsa-asg- 7.66 7.96 7.81 0.22 100 8.11 8.16 8.00 60186018_st2 hsa-asg- hsa-asg- 9.21 9.01 9.11 0.14 50 9.41 9.43 9.71 32403240_st1 hsa-asg-504 hsa-asg- 8.27 8.53 8.40 0.18 100 8.67 8.78 8.93504_st2 hsa-cand110 hsa- 10.26 10.37 10.31 0.08 100 10.77 10.67 10.67cand110_st2 hsa-asg- hsa-asg- 11.18 10.96 11.07 0.16 100 11.45 11.5011.40 3811 3811_st1 hsa-asg- hsa-asg- 10.24 10.74 10.49 0.35 100 11.0310.89 10.72 6398 6398_st2 hsa-asg- hsa-asg- 9.32 9.57 9.45 0.17 100 9.719.86 10.00 4731 4731_st1 hsa-asg- hsa-asg- 9.98 10.17 10.08 0.13 10010.70 10.28 10.39 7096 7096_st2 hsa-asg- hsa-asg- 8.90 8.81 8.85 0.07100 9.11 9.35 9.13 6367 6367_st1 hsa-asg- hsa-asg- 9.33 8.94 9.13 0.2850 9.32 9.64 9.52 3456 3456_st2 hsa-asg- hsa-asg- 6.21 6.28 6.24 0.05100 6.31 6.80 6.71 1641 1641_st1 hsa-asg- hsa-asg- 9.41 9.82 9.61 0.29100 10.16 9.86 9.98 2980 2980_st2 hsa-asg- hsa-asg- 8.10 8.57 8.34 0.34100 8.96 8.78 8.70 4828 4828_st1 hsa-asg- hsa-asg- 8.72 8.84 8.78 0.09100 9.13 9.19 9.42 9266 9266_st1 hsa-asg- hsa-asg- 10.39 10.26 10.320.09 100 10.63 10.81 10.78 3260 3260_st2 hsa-asg- hsa-asg- 7.87 7.587.72 0.21 100 7.91 8.32 8.19 6802 6802_st1 hsa-asg- hsa-asg- 5.57 5.395.48 0.12 0 5.76 6.18 5.84 7579 7579_st2 hsa-asg- hsa-asg- 3.71 3.963.84 0.17 0 4.08 4.63 4.42 12857 12857_st1 hsa-asg- hsa-asg- 7.98 8.028.00 0.02 100 8.17 8.41 8.26 8421 8421_st1 hsa-cand583 hsa- 5.62 5.215.42 0.29 0 6.20 5.85 5.61 cand583_st1 hsa-asg- hsa-asg- 8.51 8.81 8.660.21 100 9.32 8.93 9.13 1123 1123_st1 hsa-asg- hsa-asg- 9.18 8.72 8.950.33 100 9.30 9.46 9.43 11946 11946_st1 hsa-asg- hsa-asg- 6.56 6.87 6.710.22 100 6.96 7.36 7.26 12953 12953_st2 hsa-asg- hsa-asg- 8.19 7.78 7.990.29 100 8.23 8.32 8.52 10639 10639_st1 hsa-asg- hsa-asg- 7.84 7.49 7.660.25 100 7.93 7.85 8.20 3345 3345_st1 hsa-asg- hsa-asg- 7.20 7.26 7.230.04 100 7.65 7.81 7.75 3230 3230_st1 hsa-cand441 hsa- 7.25 6.85 7.050.28 0 7.18 7.74 7.51 cand441_st2 hsa-asg- hsa-asg- 10.32 9.87 10.090.32 100 10.62 10.36 10.48 3892 3892_st2 hsa-asg- hsa-asg- 6.71 6.476.59 0.17 100 7.01 7.18 7.12 7326 7326_st1 hsa-asg- hsa-asg- 6.73 7.126.92 0.28 100 7.31 7.29 7.58 1174 1174_st2 hsa-asg- hsa-asg- 6.52 6.646.58 0.09 0 6.90 7.24 7.57 3290 3290_st1 hsa-asg- hsa-asg- 7.93 7.497.71 0.31 50 8.12 8.40 8.21 12441 12441_st1 hsa-asg- hsa-asg- 7.20 7.247.22 0.03 100 7.68 7.82 7.40 4502 4502_st1 hsa-asg- hsa-asg- 8.77 8.908.84 0.09 50 9.38 9.41 9.31 11758 11758_st2 hsa-asg- hsa-asg- 6.73 6.446.58 0.21 100 6.80 7.05 7.29 4759 4759_st1 hsa-asg- hsa-asg- 7.10 7.097.09 0.01 0 7.35 7.37 7.65 3808 3808_st1 hsa-asg- hsa-asg- 7.58 7.737.66 0.10 100 7.99 8.37 8.11 11249 11249_st2 hsa-asg- hsa-asg- 8.98 9.379.18 0.28 100 9.76 9.63 9.44 6728 6728_st2 hsa-asg- hsa-asg- 7.56 6.897.23 0.47 100 7.80 7.71 7.68 2891 2891_st1 hsa-asg- hsa-asg- 4.70 4.164.43 0.38 0 4.80 4.74 4.83 14186 14186_st2 hsa-cand735 hsa- 7.49 7.477.48 0.01 100 7.93 8.02 7.94 cand735_st2 hsa-asg- hsa-asg- 4.90 5.094.99 0.14 0 5.52 5.70 5.73 8391 8391_st1 hsa-asg- hsa-asg- 5.86 6.105.98 0.17 0 6.32 6.56 6.56 1617 1617_st2 hsa-asg- hsa-asg- 8.13 8.108.11 0.02 100 8.81 8.50 8.73 2626 2626_st1 hsa-asg- hsa-asg- 6.60 6.126.36 0.34 50 6.79 6.76 6.95 11252 11252_st2 hsa-asg- hsa-asg- 4.75 4.494.62 0.18 0 5.17 5.36 5.81 11585 11585_st2 hsa-asg- hsa-asg- 7.14 6.897.01 0.18 100 7.57 7.64 7.76 10575 10575_st1 hsa-asg- hsa-asg- 6.81 6.806.81 0.00 100 7.27 7.18 7.19 14066 14066_st2 hsa-asg- hsa-asg- 7.98 7.877.92 0.08 50 8.22 8.27 8.37 11729 11729_st2 hsa-asg- hsa-asg- 6.97 7.437.20 0.33 100 7.83 7.73 7.43 11521 11521_st1 hsa-asg- hsa-asg- 8.03 8.648.33 0.43 100 9.11 8.89 8.82 12581 12581_st1 hsa-asg- hsa-asg- 8.82 8.728.77 0.07 50 9.35 9.21 9.40 13079 13079_st2 hsa-asg- hsa-asg- 4.41 4.964.68 0.39 0 5.14 5.52 5.33 7569 7569_st1 hsa-asg- hsa-asg- 8.56 8.098.32 0.34 100 8.83 9.16 8.82 3462 3462_st2 hsa-asg- hsa-asg- 6.75 6.016.38 0.53 50 6.57 6.97 7.38 8227 8227_st2 hsa-asg-508 hsa-asg- 5.94 5.785.86 0.11 50 6.23 5.99 6.52 508_st2 hsa-asg- hsa-asg- 7.51 6.80 7.160.50 100 7.57 7.73 7.66 2580 2580_st1 hsa-asg- hsa-asg- 7.13 6.82 6.980.22 100 7.04 7.56 7.87 7687 7687_st2 hsa-asg-688 hsa-asg- 6.92 7.217.06 0.21 100 7.55 7.65 7.68 688_st2 hsa-asg- hsa-asg- 7.43 7.34 7.390.06 100 7.59 8.05 8.10 3746 3746_st1 hsa-asg- hsa-asg- 4.59 5.21 4.900.44 0 5.30 5.10 5.73 2041 2041_st1 hsa-asg-819 hsa-asg- 7.40 6.85 7.120.39 100 7.52 7.25 7.91 819_st1 hsa-asg- hsa-asg- 4.27 4.49 4.38 0.15 04.88 4.79 5.21 4200 4200_st1 hsa-asg- hsa-asg- 6.78 6.32 6.55 0.32 1007.05 7.32 7.53 4469 4469_st1 hsa-asg- hsa-asg- 4.53 4.49 4.51 0.03 04.71 5.14 5.25 10183 10183_st1 hsa-cand523 hsa- 5.57 5.34 5.45 0.16 05.74 6.06 6.03 cand523_st1 hsa-asg- hsa-asg- 6.17 5.36 5.77 0.58 0 6.006.57 6.33 8393 8393_st1 hsa-asg- hsa-asg- 6.03 5.98 6.00 0.04 50 6.646.82 6.96 9729 9729_st2 hsa-asg- hsa-asg- 5.68 6.19 5.94 0.36 0 6.616.46 6.75 6675 6675_st1 hsa-asg- hsa-asg- 7.48 7.40 7.44 0.06 100 7.998.18 7.69 1813 1813_st2 hsa-asg- hsa-asg- 5.79 5.63 5.71 0.11 0 6.356.35 6.39 8562 8562_st1 hsa-asg- hsa-asg- 6.74 6.82 6.78 0.05 100 6.747.67 7.77 11479 11479_st2 hsa-asg- hsa-asg- 8.60 8.51 8.55 0.06 0 9.119.03 8.99 5379 5379_st2 hsa-asg- hsa-asg- 5.60 5.52 5.56 0.05 0 5.915.87 6.71 11563 11563_st2 hsa-asg- hsa-asg- 6.64 6.39 6.52 0.18 100 7.217.20 7.17 2131 2131_st2 hsa-asg- hsa-asg- 6.39 5.66 6.03 0.52 50 6.646.68 7.01 5289 5289_st2 hsa-asg- hsa-asg- 6.05 5.83 5.94 0.15 0 6.396.78 7.06 8565 8565_st1 hsa-asg- hsa-asg- 5.07 5.05 5.06 0.01 0 5.575.52 5.75 4599 4599_st1 hsa-asg- hsa-asg- 5.41 5.43 5.42 0.01 0 5.646.15 6.57 3340 3340_st2 hsa-asg- hsa-asg- 5.41 5.97 5.69 0.39 0 6.396.28 6.30 3694 3694_st1 hsa-asg- hsa-asg- 11.92 11.41 11.66 0.36 10012.17 12.52 12.61 9530 9530_st2 hsa-asg- hsa-asg- 7.46 7.04 7.25 0.29100 7.58 8.08 8.06 5085 5085_st2 hsa-asg- hsa-asg- 6.29 6.00 6.14 0.20 06.56 7.00 6.96 2588 2588_st1 hsa-asg- hsa-asg- 6.48 6.15 6.31 0.23 1006.78 7.18 7.10 5061 5061_st1 hsa-asg- hsa-asg- 7.43 7.40 7.42 0.02 08.36 7.91 7.99 11155 11155_st1 hsa-asg- hsa-asg- 3.52 3.05 3.28 0.33 04.08 3.88 4.05 8672 8672_st1 hsa-asg- hsa-asg- 6.56 5.99 6.27 0.40 506.63 7.00 7.14 1576 1576_st1 hsa-asg- hsa-asg- 5.81 5.36 5.59 0.32 05.55 6.17 6.49 7730 7730_st2 hsa-asg- hsa-asg- 9.32 8.80 9.06 0.37 5010.04 9.84 9.80 12247 12247_st2 hsa-asg- hsa-asg- 6.49 6.55 6.52 0.04100 6.86 7.10 7.00 9511 9511_st2 hsa-asg- hsa-asg- 5.19 5.39 5.29 0.14 05.95 6.14 5.93 8959 8959_st2 hsa-asg-797 hsa-asg- 4.75 4.43 4.59 0.22 05.33 5.36 5.13 797_st2 hsa-asg-271 hsa-asg- 3.71 3.74 3.73 0.02 0 4.204.51 4.28 271_st1 hsa-asg- hsa-asg- 11.20 10.32 10.76 0.62 100 11.2111.69 11.59 2750 2750_st2 hsa-asg- hsa-asg- 5.84 5.31 5.57 0.38 0 6.066.37 6.60 2693 2693_st1 hsa-asg-53 hsa-asg- 9.00 9.21 9.11 0.15 100 9.819.77 9.80 53_st2 hsa-asg- hsa-asg- 8.23 9.03 8.63 0.56 100 9.13 9.169.36 5032 5032_st2 hsa-asg- hsa-asg- 6.79 6.22 6.51 0.41 50 7.17 7.197.20 6892 6892_st2 hsa-asg- hsa-asg- 6.11 6.38 6.25 0.19 50 6.77 7.106.68 1911 1911_st1 hsa-asg- hsa-asg- 5.54 6.44 5.99 0.64 50 6.53 6.126.78 8675 8675_st1 hsa-asg-809 hsa-asg- 5.30 5.55 5.43 0.18 0 5.62 6.266.23 809_st2 hsa-asg- hsa-asg- 7.56 6.67 7.12 0.63 100 7.64 7.81 7.9211893 11893_st2 hsa-asg- hsa-asg- 7.69 6.65 7.17 0.74 100 7.67 8.01 7.841861 1861_st1 hsa-asg- hsa-asg- 5.37 5.46 5.42 0.06 50 6.46 5.93 6.576651 6651_st2 hsa-asg-471 hsa-asg- 7.27 7.54 7.41 0.19 0 8.03 8.17 8.19471_st1 hsa-asg- hsa-asg- 5.27 5.03 5.15 0.17 0 5.62 6.28 6.43 1086110861_st2 hsa-asg- hsa-asg- 7.88 7.63 7.75 0.18 100 8.30 8.52 8.63 43104310_st1 hsa-asg- hsa-asg- 7.18 7.33 7.25 0.10 100 7.40 8.27 8.68 71537153_st2 hsa-asg- hsa-asg- 4.64 5.21 4.93 0.40 0 5.60 5.75 5.95 68796879_st1 hsa-asg- hsa-asg- 5.07 5.38 5.22 0.22 0 5.89 6.31 5.98 73527352_st1 hsa-asg- hsa-asg- 6.60 6.65 6.63 0.04 100 7.41 7.98 7.34 1277512775_st1 hsa-asg- hsa-asg- 7.30 6.85 7.08 0.32 100 7.93 7.89 8.08 1361313613_st2 hsa-asg- hsa-asg- 1.61 2.23 1.92 0.43 0 3.11 2.31 2.82 97489748_st1 hsa-asg- hsa-asg- 6.36 5.77 6.06 0.42 50 6.71 6.93 6.77 76407640_st2 hsa-asg- hsa-asg- 5.94 6.20 6.07 0.19 0 6.96 6.55 6.82 1097110971_st2 hsa-asg- hsa-asg- 8.17 8.80 8.49 0.44 0 9.33 9.32 8.96 60926092_st2 hsa-asg-813 hsa-asg- 4.64 4.61 4.63 0.02 0 5.30 5.46 5.81813_st2 hsa-asg- hsa-asg- 6.78 6.74 6.76 0.03 100 7.41 7.55 7.76 64376437_st1 hsa-asg- hsa-asg- 7.38 7.47 7.43 0.06 100 8.25 8.49 8.30 1294612946_st1 hsa-asg- hsa-asg- 7.69 7.66 7.67 0.02 100 8.50 8.40 8.33 69486948_st2 hsa-asg- hsa-asg- 7.08 6.56 6.82 0.37 0 7.72 7.21 7.84 1148611486_st1 hsa-cand711 hsa- 9.10 9.13 9.11 0.02 50 9.89 9.84 9.99cand711_st1 hsa-cand588 hsa- 5.91 5.66 5.79 0.18 0 6.93 6.16 6.88cand588_st1 hsa-asg-555 hsa-asg- 6.78 5.89 6.34 0.63 50 6.58 7.15 7.10555_st1 hsa-asg- hsa-asg- 6.94 6.90 6.92 0.02 0 7.80 7.41 7.52 61496149_st2 hsa-asg- hsa-asg- 7.29 6.47 6.88 0.58 0 7.41 7.78 7.54 99209920_st1 hsa-asg- hsa-asg- 6.71 5.85 6.28 0.61 50 7.14 7.04 7.25 13911391_st1 hsa-asg- hsa-asg- 5.98 6.38 6.18 0.28 50 7.03 6.43 6.89 31503150_st1 hsa-asg- hsa-asg- 6.41 5.83 6.12 0.41 50 6.79 6.89 7.57 1382913829_st2 hsa-asg- hsa-asg- 6.09 5.71 5.90 0.27 0 5.98 6.73 6.64 93829382_st2 hsa-cand855 hsa- 5.68 6.13 5.91 0.32 0 6.18 6.83 6.90cand855_st1 hsa-asg- hsa-asg- 7.05 7.15 7.10 0.07 100 7.91 7.99 7.9610716 10716_st1 hsa-asg- hsa-asg- 7.96 7.83 7.90 0.10 100 8.49 9.03 8.8011698 11698_st2 hsa-cand902 hsa- 5.27 5.11 5.19 0.11 0 5.55 6.08 6.34cand902_st1 hsa-asg- hsa-asg- 8.37 8.10 8.24 0.19 100 9.23 9.50 9.1412919 12919_st2 hsa-asg- hsa-asg- 4.41 4.43 4.42 0.02 0 5.38 5.22 4.984261 4261_st1 hsa-asg- hsa-asg- 6.19 6.41 6.30 0.15 50 7.28 7.20 7.201153 1153_st1 hsa-asg- hsa-asg- 6.00 5.01 5.51 0.70 0 6.37 6.18 6.723841 3841_st2 hsa-asg- hsa-asg- 10.83 10.20 10.52 0.45 100 10.96 11.7711.58 3231 3231_st2 hsa-asg-225 hsa-asg- 4.85 4.26 4.56 0.41 0 5.27 5.145.09 225_st1 hsa-asg- hsa-asg- 7.26 6.73 6.99 0.37 100 7.74 7.52 7.952017 2017_st2 hsa-asg- hsa-asg- 6.48 6.32 6.40 0.11 100 7.20 6.99 7.156623 6623_st2 hsa-asg- hsa-asg- 4.94 4.87 4.91 0.05 0 5.69 5.85 5.529526 9526_st2 hsa-asg- hsa-asg- 4.99 4.92 4.95 0.05 0 5.41 6.03 6.503014 3014_st2 hsa-asg- hsa-asg- 6.97 7.01 6.99 0.03 100 7.21 8.21 8.4812957 12957_st1 hsa-asg- hsa-asg- 4.05 3.55 3.80 0.36 0 4.67 5.06 5.112564 2564_st2 hsa-asg- hsa-asg- 4.34 5.23 4.79 0.63 0 5.80 5.88 5.7010473 10473_st1 hsa-asg- hsa-asg- 2.58 2.99 2.79 0.29 0 3.30 3.79 3.088303 8303_st2 hsa-asg- hsa-asg- 5.03 5.55 5.29 0.37 50 6.06 6.48 6.9111310 11310_st2 hsa-asg- hsa-asg- 5.91 5.79 5.85 0.08 0 6.63 7.49 6.516831 6831_st2 hsa-asg- hsa-asg- 7.15 6.72 6.94 0.30 0 7.19 8.37 8.5011373 11373_st1 hsa-asg- hsa-asg- 6.00 6.18 6.09 0.12 0 6.80 6.90 7.496005 6005_st1 hsa-asg- hsa-asg- 8.41 9.02 8.72 0.43 0 9.52 9.57 9.594654 4654_st1 hsa-asg- hsa-asg- 7.73 7.35 7.54 0.27 100 7.94 8.32 9.269403 9403_st2 hsa-asg- hsa-asg- 7.75 7.64 7.70 0.07 100 8.93 8.38 8.317893 7893_st2 hsa-asg- hsa-asg- 7.48 6.81 7.15 0.48 100 7.51 8.29 8.6213000 13000_st1 hsa-asg- hsa-asg- 6.00 5.86 5.93 0.10 50 6.32 7.51 7.2310883 10883_st1 hsa-asg- hsa-asg- 4.75 5.38 5.06 0.44 0 5.89 5.83 5.931689 1689_st2 hsa-asg- hsa-asg- 6.57 5.59 6.08 0.70 50 6.49 7.32 7.529412 9412_st2 hsa-asg- hsa-asg- 7.97 7.34 7.66 0.45 100 8.57 8.51 8.533271 3271_st1 hsa-asg- hsa-asg- 6.71 5.95 6.33 0.54 50 7.40 7.21 7.7513559 13559_st2 hsa-asg- hsa-asg- 3.80 3.65 3.73 0.11 0 4.26 4.51 4.5811898 11898_st2 hsa-asg- hsa-asg- 3.31 3.55 3.43 0.17 0 4.08 4.39 4.323081 3081_st1 hsa-asg- hsa-asg- 6.19 5.25 5.72 0.67 0 6.66 6.68 6.902858 2858_st1 hsa-asg-278 hsa-asg- 4.64 5.31 4.97 0.47 0 5.07 6.30 5.91278_st1 hsa-asg- hsa-asg- 7.67 7.92 7.80 0.18 100 8.63 8.64 8.76 54235423_st2 hsa-asg- hsa-asg- 4.27 4.67 4.47 0.28 0 5.27 5.56 5.85 92579257_st2 hsa-asg- hsa-asg- 6.15 5.71 5.93 0.31 50 6.44 7.32 7.10 1019710197_st2 hsa-asg- hsa-asg- 7.16 6.81 6.98 0.25 100 8.52 8.24 8.04 65076507_st2 hsa-asg- hsa-asg- 5.84 5.11 5.48 0.51 0 6.52 6.59 6.91 90159015_st1 hsa-asg- hsa-asg- 1.72 1.98 1.85 0.18 0 3.11 2.69 3.38 54605460_st2 hsa-asg- hsa-asg- 8.95 9.11 9.03 0.12 100 10.96 10.10 9.6513308 13308_st2 hsa-asg- hsa-asg- 4.27 4.99 4.63 0.50 0 5.93 5.59 5.834440 4440_st1 hsa-asg- hsa-asg- 6.41 5.52 5.97 0.63 100 7.01 7.15 7.051375 1375_st1 hsa-asg- hsa-asg- 6.17 4.96 5.57 0.85 0 6.64 6.43 6.998763 8763_st2 hsa-asg- hsa-asg- 8.47 8.68 8.58 0.15 100 9.33 9.90 10.512872 2872_st2 hsa-asg- hsa-asg- 6.63 6.63 6.63 0.00 100 7.45 8.42 8.2012460 12460_st2 hsa-asg- hsa-asg- 5.03 5.43 5.23 0.28 0 6.18 5.74 6.728138 8138_st2 hsa-asg- hsa-asg- 9.09 9.11 9.10 0.01 100 11.02 10.13 9.945304 5304_st1 hsa-asg- hsa-asg- 5.89 4.49 5.19 0.99 0 6.20 6.03 6.454971 4971_st1 hsa-asg- hsa-asg- 5.91 6.38 6.15 0.33 50 7.20 7.06 7.399343 9343_st1 hsa-asg- hsa-asg- 5.07 5.17 5.12 0.07 0 6.02 6.24 6.388271 8271_st2 hsa-asg- hsa-asg- 7.43 6.67 7.05 0.54 100 8.64 8.29 8.5613260 13260_st1 hsa-asg- hsa-asg- 4.34 4.23 4.29 0.08 0 5.41 5.90 4.5512105 12105_st2 hsa-asg- hsa-asg- 3.20 2.42 2.81 0.55 0 3.56 4.97 3.887685 7685_st2 hsa-asg- hsa-asg- 5.79 5.55 5.67 0.17 0 7.37 6.62 6.683360 3360_st2 hsa-asg- hsa-asg- 5.37 4.19 4.78 0.83 0 5.36 6.42 6.505576 5576_st1 hsa-asg- hsa-asg- 5.27 4.04 4.66 0.87 0 5.49 6.28 5.854843 4843_st2 hsa-asg- hsa-asg- 5.27 4.49 4.88 0.55 0 5.71 6.22 7.157009 7009_st1 hsa-asg- hsa-asg- 3.08 4.40 3.74 0.93 0 4.76 5.14 5.478928 8928_st2 hsa-asg- hsa-asg- 3.97 4.72 4.35 0.53 0 5.07 5.49 5.5812777 12777_st2 hsa-asg- hsa-asg- 5.23 4.61 4.92 0.44 0 5.71 6.55 7.097069 7069_st1 hsa-cand965 hsa- 6.64 5.19 5.92 1.02 0 7.25 7.94 7.66cand965_st1 hsa-asg- hsa-asg- 5.27 4.08 4.67 0.84 0 5.80 6.12 6.29 1369313693_st2 hsa-asg- hsa-asg- 5.03 4.26 4.65 0.54 0 6.06 6.18 6.03 83298329_st1 hsa-asg- hsa-asg- 5.65 4.96 5.31 0.49 0 5.89 6.67 6.69 35973597_st2 hsa-asg- hsa-asg- 5.30 3.92 4.61 0.98 0 5.55 6.43 6.33 98529852_st1 hsa-asg- hsa-asg- 3.31 2.61 2.96 0.49 0 4.53 4.39 3.97 31073107_st1 hsa-asg- hsa-asg- 10.81 10.39 10.60 0.30 100 12.93 11.30 12.1114245 14245_st1 hsa-asg- hsa-asg- 5.07 4.08 4.58 0.70 0 5.85 6.08 6.316949 6949_st1 hsa-asg- hsa-asg- 4.41 3.65 4.03 0.54 0 5.64 5.29 5.497319 7319_st2 hsa-asg- hsa-asg- 5.34 4.23 4.78 0.78 0 6.75 5.82 6.669022 9022_st2 hsa-asg- hsa-asg- 4.80 4.89 4.85 0.07 0 6.58 5.93 6.3912246 12246_st2 hsa-asg- hsa-asg- 5.84 5.91 5.87 0.05 0 6.44 8.01 8.098917 8917_st2 hsa-asg- hsa-asg- 4.94 5.55 5.25 0.43 0 7.74 7.02 7.0913244 13244_st1 hsa-cand345 hsa- 4.75 4.23 4.49 0.37 0 6.31 5.64 5.72cand345_st1 hsa-asg- hsa-asg- 4.99 6.40 5.69 1.00 50 7.42 7.02 8.5712702 12702_st1 hsa-asg- hsa-asg- 5.68 4.67 5.17 0.72 0 7.14 7.01 6.7313230 13230_st2 hsa-asg- hsa-asg- 4.59 5.70 5.14 0.79 50 7.71 7.34 6.6112902 12902_st1 hsa-asg- hsa-asg- 6.00 4.92 5.46 0.77 0 7.44 6.96 7.2613237 13237_st1 hsa-asg- hsa-asg- 5.54 5.21 5.37 0.23 0 5.85 7.63 7.944002 4002_st1 hsa-asg-21 hsa-asg- 5.11 3.45 4.28 1.18 0 5.67 5.83 6.8921_st2 hsa-asg- hsa-asg- 6.21 5.46 5.84 0.53 0 7.38 7.72 8.38 1014910149_st1 hsa-asg- hsa-asg- 6.39 3.88 5.14 1.78 50 6.51 7.95 6.78 53035303_st1 hsa-asg- hsa-asg- 5.47 3.34 4.41 1.51 0 6.16 6.85 7.05 1066610666_st1 hsa-asg-335 hsa-asg- 5.86 6.02 5.94 0.11 50 6.63 8.54 8.65335_st1 hsa-asg- hsa-asg- 3.97 4.49 4.23 0.37 0 5.38 6.36 6.71 62506250_st2 hsa-asg- hsa-asg- 4.27 3.92 4.10 0.25 0 5.44 6.38 6.78 82088208_st1 hsa-asg- hsa-asg- 5.65 5.83 5.74 0.13 50 6.32 8.35 8.75 1038710387_st1 hsa-asg- hsa-asg- 7.17 7.07 7.12 0.07 100 9.94 8.87 8.61 1324813248_st1 hsa-asg- hsa-asg- 4.34 2.74 3.54 1.13 0 4.48 6.12 6.27 1207912079_st1 hsa-asg- hsa-asg- 5.91 4.96 5.44 0.67 50 7.15 7.23 7.47 45574557_st2 hsa-asg- hsa-asg- 8.00 5.49 6.75 1.78 50 8.27 9.59 9.28 1393713937_st1 hsa-miR165 hsa- 4.20 2.55 3.38 1.17 0 4.80 5.14 5.47miR165_st1 hsa-asg- hsa-asg- 4.80 4.00 4.40 0.56 0 4.92 7.43 7.55 86828682_st2 hsa-asg- hsa-asg- 4.20 2.68 3.44 1.08 0 5.07 5.95 6.52 64136413_st2 hsa-asg- hsa-asg- 5.07 3.55 4.31 1.08 0 6.15 6.40 7.12 1330413304_st2 hsa-asg- hsa-asg- 2.84 4.43 3.63 1.13 0 4.37 6.59 6.40 42054205_st2 hsa-asg-870 hsa-asg- 3.97 3.39 3.68 0.41 0 4.37 6.85 7.49870_st1 hsa-asg- hsa-asg- 8.77 6.85 7.81 1.35 50 9.87 10.31 10.51 70747074_st1 hsa-asg- hsa-asg- 6.43 3.45 4.94 2.11 50 8.09 7.42 7.28 1327913279_st1 hsa-asg- hsa-asg- 9.47 11.07 10.27 1.13 100 3.20 5.22 3.025638 5638_st2 hsa-asg- hsa-asg- 7.15 9.46 8.30 1.63 100 4.32 3.88 3.5411338 11338_st2 hsa-asg- hsa-asg- 6.83 9.45 8.14 1.85 100 6.46 4.19 3.025630 5630_st2 hsa-asg- hsa-asg- 6.97 4.49 5.73 1.75 50 3.01 2.57 3.4913997 13997_st2 hsa-asg- hsa-asg- 6.75 4.72 5.74 1.44 100 3.01 3.06 2.768428 8428_st2 hsa-asg- hsa-asg- 7.60 8.05 7.82 0.32 100 4.26 6.07 5.359186 9186_st1 hsa-asg- hsa-asg- 6.52 4.64 5.58 1.33 50 3.20 2.94 2.621296 1296_st1 hsa-asg- hsa-asg- 5.74 5.05 5.39 0.48 100 3.01 3.29 2.369723 9723_st2 hsa-asg- hsa-asg- 6.09 4.55 5.32 1.09 50 2.80 2.69 3.5911380 11380_st2 hsa-asg- hsa-asg- 4.47 6.30 5.39 1.30 50 3.11 3.40 3.082261 2261_st1 hsa-asg- hsa-asg- 8.07 9.09 8.58 0.72 100 5.74 6.92 7.201519 1519_st1 hsa-asg- hsa-asg- 5.91 4.43 5.17 1.05 50 3.11 3.06 4.131794 1794_st1 hsa-asg- hsa-asg- 6.25 6.91 6.58 0.47 50 4.48 4.51 4.4912271 12271_st1 hsa-asg- hsa-asg- 5.44 7.37 6.41 1.37 50 3.80 3.70 4.3914208 14208_st1 hsa-asg- hsa-asg- 4.53 6.90 5.71 1.68 50 3.72 3.70 3.148486 8486_st2 hsa-asg- hsa-asg- 10.46 10.75 10.61 0.20 100 8.43 8.509.42 5640 5640_st1 hsa-cand317 hsa- 6.62 4.61 5.61 1.42 50 3.48 4.192.76 cand317_st1 hsa-asg- hsa-asg- 4.59 5.46 5.02 0.62 50 3.48 3.88 3.022335 2335_st1 hsa-asg- hsa-asg- 5.79 3.55 4.67 1.58 50 3.30 3.29 3.021587 1587_st1 hsa-asg- hsa-asg- 5.62 6.32 5.97 0.49 50 4.26 3.83 4.834803 4803_st2 hsa-asg- hsa-asg- 10.94 10.80 10.87 0.10 100 9.51 8.489.66 13278 13278_st2 hsa-asg-497 hsa-asg- 7.46 7.19 7.33 0.19 100 5.715.67 5.80 497_st2 hsa-asg- hsa-asg- 5.37 6.00 5.69 0.44 0 3.11 4.22 5.029796 9796_st1 hsa-asg- hsa-asg- 6.39 4.55 5.47 1.30 50 4.58 3.29 3.9310031 10031_st1 hsa-asg- hsa-asg- 6.00 5.55 5.78 0.32 50 4.37 4.33 3.749980 9980_st1 hsa-asg- hsa-asg- 4.94 6.89 5.91 1.37 50 4.32 4.26 4.4612453 12453_st2 hsa-asg- hsa-asg- 7.71 5.70 6.70 1.42 50 5.07 4.79 5.4511489 11489_st1 hsa-asg- hsa-asg- 4.90 4.72 4.81 0.12 0 2.90 3.18 3.3811361 11361_st2 hsa-asg- hsa-asg- 7.18 6.67 6.93 0.36 100 5.14 5.02 5.4012807 12807_st2 hsa-asg- hsa-asg- 6.44 6.14 6.29 0.22 100 4.71 4.57 4.837511 7511_st2 hsa-asg- hsa-asg- 6.93 5.21 6.07 1.21 50 4.84 4.88 5.079762 9762_st1 hsa-asg- hsa-asg- 7.39 8.00 7.69 0.43 50 5.62 6.44 6.8412372 12372_st2 hsa-asg- hsa-asg- 9.22 8.68 8.95 0.38 100 8.20 6.66 8.114223 4223_st2 hsa-asg- hsa-asg- 7.02 6.29 6.65 0.52 100 5.98 5.56 5.6111402 11402_st1 hsa-asg- hsa-asg- 7.79 7.46 7.62 0.23 100 6.52 6.33 6.468633 8633_st1 hsa-asg- hsa-asg- 7.21 6.62 6.91 0.42 100 5.23 6.18 5.944780 4780_st1 hsa-asg- hsa-asg- 6.98 6.99 6.98 0.01 100 5.47 5.84 5.799117 9117_st1 hsa-asg- hsa-asg- 6.19 5.29 5.74 0.64 50 4.84 4.04 4.211362 1362_st2 hsa-asg- hsa-asg- 10.75 10.40 10.57 0.25 100 8.77 9.789.09 14233 14233_st1 hsa-asg- hsa-asg- 13.14 12.47 12.80 0.47 100 12.3811.41 11.96 13309 13309_st2 hsa-asg- hsa-asg- 8.42 7.09 7.76 0.94 1006.86 6.57 6.69 13841 13841_st2 hsa-asg- hsa-asg- 7.42 7.26 7.34 0.11 1005.78 6.08 6.22 14001 14001_st1 hsa-asg- hsa-asg- 5.65 5.64 5.65 0.01 504.58 5.14 4.80 12320 12320_st2 hsa-asg- hsa-asg- 8.14 7.07 7.61 0.76 1006.35 6.73 6.63 9227 9227_st1 hsa-asg- hsa-asg- 7.30 7.00 7.15 0.21 1005.97 6.18 6.36 7922 7922_st2 hsa-asg- hsa-asg- 9.19 9.35 9.27 0.11 1007.76 8.44 8.32 9261 9261_st1 hsa-cand732 hsa- 7.47 8.16 7.82 0.49 1006.44 6.34 6.99 cand732_st2 hsa-asg- hsa-asg- 6.05 6.04 6.05 0.00 50 5.305.06 4.72 13924 13924_st1 hsa-asg- hsa-asg- 5.84 5.46 5.65 0.27 0 4.144.97 4.83 2889 2889_st2 hsa-asg- hsa-asg- 6.79 7.43 7.11 0.45 100 5.745.90 6.09 12381 12381_st1 hsa-asg- hsa-asg- 7.36 7.75 7.56 0.28 100 6.536.64 6.31 8003 8003_st2 hsa-asg- hsa-asg- 6.51 5.64 6.08 0.61 100 4.805.18 4.64 7262 7262_st1 hsa-asg- hsa-asg- 10.31 11.71 11.01 0.99 1009.65 9.98 10.04 11850 11850_st1 hsa-asg- hsa-asg- 6.60 6.87 6.74 0.19100 5.93 5.87 5.70 7027 7027_st2 hsa-asg- hsa-asg- 8.58 9.10 8.84 0.37100 7.89 7.38 8.02 12937 12937_st2 hsa-asg-345 hsa-asg- 5.60 5.75 5.680.11 50 4.84 4.74 5.02 345_st1 hsa-asg- hsa-asg- 6.63 6.13 6.38 0.35 1006.34 5.26 5.38 4969 4969_st2 hsa-asg- hsa-asg- 6.92 5.88 6.40 0.73 505.17 5.64 5.64 3798 3798_st1 hsa-asg- hsa-asg- 6.89 7.41 7.15 0.37 1005.91 6.08 5.97 4703 4703_st2 hsa-asg- hsa-asg- 4.59 3.55 4.07 0.73 03.48 2.19 2.95 8792 8792_st1 hsa-asg- hsa-asg- 5.89 6.10 5.99 0.15 504.58 5.18 5.61 4460 4460_st2 hsa-asg- hsa-asg- 7.83 8.30 8.06 0.33 1006.57 7.31 7.25 4026 4026_st1 hsa-cand421 hsa- 11.03 12.08 11.56 0.75 10010.27 10.91 10.45 cand421_st1 hsa-asg- hsa-asg- 8.43 8.61 8.52 0.13 1006.97 7.90 7.97 3670 3670_st1 hsa-asg- hsa-asg- 7.83 8.38 8.10 0.39 1007.00 7.33 7.12 9130 9130_st2 hsa-asg- hsa-asg- 5.65 6.97 6.31 0.93 505.57 5.43 5.85 7783 7783_st1 hsa-asg- hsa-asg- 5.79 6.48 6.13 0.49 505.10 5.85 4.98 3944 3944_st1 hsa-asg- hsa-asg- 5.74 5.83 5.78 0.07 504.53 5.14 4.88 13798 13798_st1 hsa-asg- hsa-asg- 8.85 9.09 8.97 0.16 1007.58 7.87 8.40 6870 6870_st1 hsa-asg- hsa-asg- 5.41 6.00 5.70 0.42 504.20 4.93 4.72 2460 2460_st1 hsa-asg- hsa-asg- 4.70 5.86 5.28 0.82 504.32 4.74 4.61 11267 11267_st2 hsa-asg- hsa-asg- 6.48 6.11 6.29 0.26 1005.23 5.43 5.31 3604 3604_st1 hsa-asg- hsa-asg- 6.93 6.77 6.85 0.11 1005.98 5.99 5.73 4296 4296_st1 hsa-asg- hsa-asg- 8.56 9.33 8.95 0.54 1007.81 8.41 8.26 8866 8866_st1 hsa-asg- hsa-asg- 5.86 5.36 5.61 0.36 1004.67 5.06 4.93 12471 12471_st1 hsa-asg- hsa-asg- 8.55 7.89 8.22 0.46 1007.07 7.29 7.77 11515 11515_st2 hsa-asg- hsa-asg- 6.21 6.81 6.51 0.42 505.71 5.61 6.20 7252 7252_st1 hsa-asg- hsa-asg- 6.52 6.52 6.52 0.00 1006.31 5.64 6.14 11458 11458_st2 hsa-asg- hsa-asg- 9.17 9.63 9.40 0.33 508.38 8.93 8.47 13453 13453_st1 hsa-asg- hsa-asg- 9.98 10.07 10.02 0.07100 9.11 9.16 9.23 3722 3722_st1 hsa-asg- hsa-asg- 11.02 12.01 11.520.69 100 10.84 10.88 10.76 3992 3992_st2 hsa-asg- hsa-asg- 6.75 7.247.00 0.35 100 6.54 6.44 6.23 4373 4373_st1 hsa-asg- hsa-asg- 7.74 7.747.74 0.00 100 6.91 7.02 7.01 9480 9480_st1 hsa-asg- hsa-asg- 12.87 13.6013.24 0.52 100 12.55 12.77 12.46 7939 7939_st1 hsa-asg- hsa-asg- 6.976.76 6.86 0.15 100 6.16 6.12 6.47 4448 4448_st1 hsa-asg- hsa-asg- 6.627.06 6.84 0.31 100 6.41 6.12 6.50 1338 1338_st2 hsa-asg- hsa-asg- 8.118.21 8.16 0.07 100 7.33 7.29 7.45 11617 11617_st2 hsa-asg-954 hsa-asg-9.97 9.90 9.93 0.05 100 9.28 9.11 9.34 954_st1 hsa-asg- hsa-asg- 6.496.76 6.63 0.19 100 6.04 6.16 5.64 2381 2381_st2 hsa-asg- hsa-asg- 5.896.04 5.97 0.11 50 5.87 5.02 5.40 2697 2697_st1 hsa-asg- hsa-asg- 6.156.34 6.25 0.13 50 5.60 5.52 5.84 2365 2365_st1 hsa-asg- hsa-asg- 5.375.98 5.68 0.43 50 5.00 5.02 5.09 9795 9795_st2 hsa-asg- hsa-asg- 5.305.75 5.53 0.32 50 4.48 4.88 4.67 12457 12457_st2 hsa-asg- hsa-asg- 6.366.35 6.35 0.01 100 5.52 5.83 5.94 2500 2500_st1 hsa-asg- hsa-asg- 6.947.85 7.40 0.64 100 6.68 6.97 7.02 8882 8882_st1 hsa-asg- hsa-asg- 6.035.83 5.93 0.14 100 5.36 5.10 5.11 5973 5973_st1 hsa-asg- hsa-asg- 6.436.80 6.61 0.26 100 5.93 6.59 5.98 6616 6616_st1 hsa-asg- hsa-asg- 6.676.97 6.82 0.21 100 6.42 6.33 6.32 13294 13294_st2 hsa-asg- hsa-asg- 5.475.49 5.48 0.01 100 5.00 4.93 4.35 9521 9521_st2 hsa-asg- hsa-asg- 8.949.21 9.08 0.19 100 8.48 8.25 8.53 7450 7450_st1 hsa-asg- hsa-asg- 4.905.17 5.04 0.20 50 4.53 4.33 4.42 4030 4030_st2 hsa-asg- hsa-asg- 7.277.69 7.48 0.30 100 6.64 6.89 7.15 12406 12406_st1 hsa-asg- hsa-asg- 6.136.11 6.12 0.02 50 5.67 5.90 5.76 12885 12885_st1 hsa-cand873 hsa- 5.865.93 5.90 0.05 100 5.41 5.29 5.02 cand873_st1 hsa-asg- hsa-asg- 13.5114.05 13.78 0.38 100 13.37 13.40 13.27 14201 14201_st2 hsa-asg- hsa-asg-10.48 11.07 10.78 0.42 100 10.50 10.37 10.00 8568 8568_st1 hsa-asg-hsa-asg- 7.88 8.39 8.13 0.36 100 7.57 7.54 7.68 3239 3239_st2 hsa-asg-hsa-asg- 7.66 7.57 7.62 0.07 100 7.19 7.01 7.12 4984 4984_st1 hsa-asg-hsa-asg- 6.32 6.13 6.23 0.14 100 5.98 5.64 5.80 2093 2093_st1 hsa-asg-6.38 6.41 6.39 0.02 100 5.82 6.28 6.01 hsa-asg-733 733_st1 hsa-asg-89hsa-asg- 6.52 6.58 6.55 0.04 100 6.00 6.26 6.17 89_st2 hsa-asg- hsa-asg-6.19 6.04 6.12 0.11 100 5.78 5.56 5.89 12816 12816_st1 hsa-asg- hsa-asg-5.89 5.79 5.84 0.07 0 5.27 5.70 5.61 11057 11057_st1 hsa-asg- hsa-asg-6.67 6.46 6.57 0.15 100 6.11 6.30 6.17 6305 6305_st1 hsa-asg- hsa-asg-5.86 6.03 5.95 0.12 50 5.95 5.59 5.49 4587 4587_st1 hsa-asg- hsa-asg-6.03 6.13 6.08 0.07 50 5.85 6.02 5.92 2483 2483_st2 hsa-asg-758 hsa-asg-6.25 6.20 6.23 0.04 50 6.51 6.71 6.34 758_st1 hsa-asg- hsa-asg- 6.346.29 6.31 0.04 100 6.38 6.59 6.82 7410 7410_st1 hsa-asg- hsa-asg- 9.8710.06 9.97 0.14 100 10.37 10.21 10.17 2899 2899_st2 hsa-asg- hsa-asg-9.14 8.83 8.99 0.21 50 9.10 9.21 9.44 7628 7628_st2 hsa-asg- hsa-asg-10.36 10.48 10.42 0.09 100 10.76 10.52 10.96 14034 14034_st1 hsa-asg-hsa-asg- 9.65 9.45 9.55 0.14 100 9.69 9.77 9.89 8253 8253_st2 hsa-asg-hsa-asg- 4.59 4.55 4.57 0.02 0 4.76 5.18 4.72 4426 4426_st2 hsa-asg-hsa-asg- 6.59 6.14 6.36 0.32 50 6.52 6.73 6.78 8655 8655_st1 hsa-asg-hsa-asg- 10.98 11.15 11.07 0.12 100 11.43 11.30 11.51 10632 10632_st1hsa-asg-974 hsa-asg- 6.60 6.60 6.60 0.00 100 6.71 7.06 7.30 974_st2hsa-asg- hsa-asg- 6.15 6.02 6.09 0.09 0 6.32 6.46 6.60 7218 7218_st1hsa-asg-206 hsa-asg- 9.59 9.13 9.36 0.33 50 9.82 9.68 9.76 206_st2hsa-asg- hsa-asg- 7.44 7.52 7.48 0.06 0 7.64 7.74 8.17 3565 3565_st2hsa-cand559 hsa- 6.88 7.08 6.98 0.14 100 7.13 7.31 7.70 cand559_st1hsa-asg- hsa-asg- 8.00 7.96 7.98 0.03 100 8.67 8.09 8.48 1809 1809_st1hsa-asg- hsa-asg- 8.37 8.28 8.32 0.07 100 8.63 8.61 8.74 4020 4020_st2hsa-asg- hsa-asg- 7.95 7.76 7.86 0.14 100 8.02 8.21 8.42 10392 10392_st1hsa-asg- hsa-asg- 6.07 6.15 6.11 0.06 0 6.32 6.60 6.49 9501 9501_st1hsa-asg-976 hsa-asg- 6.27 6.18 6.22 0.06 50 6.29 6.82 6.71 976_st2hsa-asg- hsa-asg- 9.27 9.72 9.49 0.32 100 9.97 9.92 9.87 5749 5749_st2hsa-asg- hsa-asg- 8.63 9.06 8.84 0.30 100 9.43 9.12 9.14 8631 8631_st1hsa-asg- hsa-asg- 8.92 8.89 8.90 0.02 50 9.32 9.13 9.24 7051 7051_st1hsa-asg- hsa-asg- 5.30 5.95 5.63 0.46 50 6.16 5.78 6.06 10506 10506_st2hsa-asg-507 hsa-asg- 2.58 2.61 2.60 0.02 0 3.11 2.82 2.89 507_st1hsa-asg- hsa-asg- 7.66 7.96 7.81 0.22 100 8.11 8.16 8.00 6018 6018_st2hsa-asg- hsa-asg- 9.21 9.01 9.11 0.14 50 9.41 9.43 9.71 3240 3240_st1hsa-asg-504 hsa-asg- 8.27 8.53 8.40 0.18 100 8.67 8.78 8.93 504_st2hsa-cand110 hsa- 10.26 10.37 10.31 0.08 100 10.77 10.67 10.67cand110_st2 hsa-asg- hsa-asg- 11.18 10.96 11.07 0.16 100 11.45 11.5011.40 3811 3811_st1 hsa-asg- hsa-asg- 10.24 10.74 10.49 0.35 100 11.0310.89 10.72 6398 6398_st2 hsa-asg- hsa-asg- 9.32 9.57 9.45 0.17 100 9.719.86 10.00 4731 4731_st1 hsa-asg- hsa-asg- 9.98 10.17 10.08 0.13 10010.70 10.28 10.39 7096 7096_st2 hsa-asg- hsa-asg- 8.90 8.81 8.85 0.07100 9.11 9.35 9.13 6367 6367_st1 hsa-asg- hsa-asg- 9.33 8.94 9.13 0.2850 9.32 9.64 9.52 3456 3456_st2 hsa-asg- hsa-asg- 6.21 6.28 6.24 0.05100 6.31 6.80 6.71 1641 1641_st1 hsa-asg- hsa-asg- 9.41 9.82 9.61 0.29100 10.16 9.86 9.98 2980 2980_st2 hsa-asg- hsa-asg- 8.10 8.57 8.34 0.34100 8.96 8.78 8.70 4828 4828_st1 hsa-asg- hsa-asg- 8.72 8.84 8.78 0.09100 9.13 9.19 9.42 9266 9266_st1 hsa-asg- hsa-asg- 10.39 10.26 10.320.09 100 10.63 10.81 10.78 3260 3260_st2 hsa-asg- hsa-asg- 7.87 7.587.72 0.21 100 7.91 8.32 8.19 6802 6802_st1 hsa-asg- hsa-asg- 5.57 5.395.48 0.12 0 5.76 6.18 5.84 7579 7579_st2 hsa-asg- hsa-asg- 3.71 3.963.84 0.17 0 4.08 4.63 4.42 12857 12857_st1 hsa-asg- hsa-asg- 7.98 8.028.00 0.02 100 8.17 8.41 8.26 8421 8421_st1 hsa-cand583 hsa- 5.62 5.215.42 0.29 0 6.20 5.85 5.61 cand583_st1 hsa-asg- hsa-asg- 8.51 8.81 8.660.21 100 9.32 8.93 9.13 1123 1123_st1 hsa-asg- hsa-asg- 9.18 8.72 8.950.33 100 9.30 9.46 9.43 11946 11946_st1 hsa-asg- hsa-asg- 6.56 6.87 6.710.22 100 6.96 7.36 7.26 12953 12953_st2 hsa-asg- hsa-asg- 8.19 7.78 7.990.29 100 8.23 8.32 8.52 10639 10639_st1 hsa-asg- hsa-asg- 7.84 7.49 7.660.25 100 7.93 7.85 8.20 3345 3345_st1 hsa-asg- hsa-asg- 7.20 7.26 7.230.04 100 7.65 7.81 7.75 3230 3230_st1 hsa-cand441 hsa- 7.25 6.85 7.050.28 0 7.18 7.74 7.51 cand441_st2 hsa-asg- hsa-asg- 10.32 9.87 10.090.32 100 10.62 10.36 10.48 3892 3892_st2 hsa-asg- hsa-asg- 6.71 6.476.59 0.17 100 7.01 7.18 7.12 7326 7326_st1 hsa-asg- hsa-asg- 6.73 7.126.92 0.28 100 7.31 7.29 7.58 1174 1174_st2 hsa-asg- hsa-asg- 6.52 6.646.58 0.09 0 6.90 7.24 7.57 3290 3290_st1 hsa-asg- hsa-asg- 7.93 7.497.71 0.31 50 8.12 8.40 8.21 12441 12441_st1 hsa-asg- hsa-asg- 7.20 7.247.22 0.03 100 7.68 7.82 7.40 4502 4502_st1 hsa-asg- hsa-asg- 8.77 8.908.84 0.09 50 9.38 9.41 9.31 11758 11758_st2 hsa-asg- hsa-asg- 6.73 6.446.58 0.21 100 6.80 7.05 7.29 4759 4759_st1 hsa-asg- hsa-asg- 7.10 7.097.09 0.01 0 7.35 7.37 7.65 3808 3808_st1 hsa-asg- hsa-asg- 7.58 7.737.66 0.10 100 7.99 8.37 8.11 11249 11249_st2 hsa-asg- hsa-asg- 8.98 9.379.18 0.28 100 9.76 9.63 9.44 6728 6728_st2 hsa-asg- hsa-asg- 7.56 6.897.23 0.47 100 7.80 7.71 7.68 2891 2891_st1 hsa-asg- hsa-asg- 4.70 4.164.43 0.38 0 4.80 4.74 4.83 14186 14186_st2 hsa-cand735 hsa- 7.49 7.477.48 0.01 100 7.93 8.02 7.94 cand735_st2 hsa-asg- hsa-asg- 4.90 5.094.99 0.14 0 5.52 5.70 5.73 8391 8391_st1 hsa-asg- hsa-asg- 5.86 6.105.98 0.17 0 6.32 6.56 6.56 1617 1617_st2 hsa-asg- hsa-asg- 8.13 8.108.11 0.02 100 8.81 8.50 8.73 2626 2626_st1 hsa-asg- hsa-asg- 6.60 6.126.36 0.34 50 6.79 6.76 6.95 11252 11252_st2 hsa-asg- hsa-asg- 4.75 4.494.62 0.18 0 5.17 5.36 5.81 11585 11585_st2 hsa-asg- hsa-asg- 7.14 6.897.01 0.18 100 7.57 7.64 7.76 10575 10575_st1 hsa-asg- hsa-asg- 6.81 6.806.81 0.00 100 7.27 7.18 7.19 14066 14066_st2 hsa-asg- hsa-asg- 7.98 7.877.92 0.08 50 8.22 8.27 8.37 11729 11729_st2 hsa-asg- hsa-asg- 6.97 7.437.20 0.33 100 7.83 7.73 7.43 11521 11521_st1 hsa-asg- hsa-asg- 8.03 8.648.33 0.43 100 9.11 8.89 8.82 12581 12581_st1 hsa-asg- hsa-asg- 8.82 8.728.77 0.07 50 9.35 9.21 9.40 13079 13079_st2 hsa-asg- hsa-asg- 4.41 4.964.68 0.39 0 5.14 5.52 5.33 7569 7569_st1 hsa-asg- hsa-asg- 8.56 8.098.32 0.34 100 8.83 9.16 8.82 3462 3462_st2 hsa-asg- hsa-asg- 6.75 6.016.38 0.53 50 6.57 6.97 7.38 8227 8227_st2 hsa-asg-508 hsa-asg- 5.94 5.785.86 0.11 50 6.23 5.99 6.52 508_st2 hsa-asg- hsa-asg- 7.51 6.80 7.160.50 100 7.57 7.73 7.66 2580 2580_st1 hsa-asg- hsa-asg- 7.13 6.82 6.980.22 100 7.04 7.56 7.87 7687 7687_st2 hsa-asg-688 hsa-asg- 6.92 7.217.06 0.21 100 7.55 7.65 7.68 688_st2 hsa-asg- hsa-asg- 7.43 7.34 7.390.06 100 7.59 8.05 8.10 3746 3746_st1 hsa-asg- hsa-asg- 4.59 5.21 4.900.44 0 5.30 5.10 5.73 2041 2041_st1 hsa-asg-819 hsa-asg- 7.40 6.85 7.120.39 100 7.52 7.25 7.91 819_st1 hsa-asg- hsa-asg- 4.27 4.49 4.38 0.15 04.88 4.79 5.21 4200 4200_st1 hsa-asg- hsa-asg- 6.78 6.32 6.55 0.32 1007.05 7.32 7.53 4469 4469_st1 hsa-asg- hsa-asg- 4.53 4.49 4.51 0.03 04.71 5.14 5.25 10183 10183_st1 hsa-cand523 hsa- 5.57 5.34 5.45 0.16 05.74 6.06 6.03 cand523_st1 hsa-asg- hsa-asg- 6.17 5.36 5.77 0.58 0 6.006.57 6.33 8393 8393_st1 hsa-asg- hsa-asg- 6.03 5.98 6.00 0.04 50 6.646.82 6.96 9729 9729_st2 hsa-asg- hsa-asg- 5.68 6.19 5.94 0.36 0 6.616.46 6.75 6675 6675_st1 hsa-asg- hsa-asg- 7.48 7.40 7.44 0.06 100 7.998.18 7.69 1813 1813_st2 hsa-asg- hsa-asg- 5.79 5.63 5.71 0.11 0 6.356.35 6.39 8562 8562_st1 hsa-asg- hsa-asg- 6.74 6.82 6.78 0.05 100 6.747.67 7.77 11479 11479_st2 hsa-asg- hsa-asg- 8.60 8.51 8.55 0.06 0 9.119.03 8.99 5379 5379_st2 hsa-asg- hsa-asg- 5.60 5.52 5.56 0.05 0 5.915.87 6.71 11563 11563_st2 hsa-asg- hsa-asg- 6.64 6.39 6.52 0.18 100 7.217.20 7.17 2131 2131_st2 hsa-asg- hsa-asg- 6.39 5.66 6.03 0.52 50 6.646.68 7.01 5289 5289_st2 hsa-asg- hsa-asg- 6.05 5.83 5.94 0.15 0 6.396.78 7.06 8565 8565_st1 hsa-asg- hsa-asg- 5.07 5.05 5.06 0.01 0 5.575.52 5.75 4599 4599_st1 hsa-asg- hsa-asg- 5.41 5.43 5.42 0.01 0 5.646.15 6.57 3340 3340_st2 hsa-asg- hsa-asg- 5.41 5.97 5.69 0.39 0 6.396.28 6.30 3694 3694_st1 hsa-asg- hsa-asg- 11.92 11.41 11.66 0.36 10012.17 12.52 12.61 9530 9530_st2 hsa-asg- hsa-asg- 7.46 7.04 7.25 0.29100 7.58 8.08 8.06 5085 5085_st2 hsa-asg- hsa-asg- 6.29 6.00 6.14 0.20 06.56 7.00 6.96 2588 2588_st1 hsa-asg- hsa-asg- 6.48 6.15 6.31 0.23 1006.78 7.18 7.10 5061 5061_st1 hsa-asg- hsa-asg- 7.43 7.40 7.42 0.02 08.36 7.91 7.99 11155 11155_st1 hsa-asg- hsa-asg- 3.52 3.05 3.28 0.33 04.08 3.88 4.05 8672 8672_st1 hsa-asg- hsa-asg- 6.56 5.99 6.27 0.40 506.63 7.00 7.14 1576 1576_st1 hsa-asg- hsa-asg- 5.81 5.36 5.59 0.32 05.55 6.17 6.49 7730 7730_st2 hsa-asg- hsa-asg- 9.32 8.80 9.06 0.37 5010.04 9.84 9.80 12247 12247_st2 hsa-asg- hsa-asg- 6.49 6.55 6.52 0.04100 6.86 7.10 7.00 9511 9511_st2 hsa-asg- hsa-asg- 5.19 5.39 5.29 0.14 05.95 6.14 5.93 8959 8959_st2 hsa-asg-797 hsa-asg- 4.75 4.43 4.59 0.22 05.33 5.36 5.13 797_st2 hsa-asg-271 hsa-asg- 3.71 3.74 3.73 0.02 0 4.204.51 4.28 271_st1 hsa-asg- hsa-asg- 11.20 10.32 10.76 0.62 100 11.2111.69 11.59 2750 2750_st2 hsa-asg- hsa-asg- 5.84 5.31 5.57 0.38 0 6.066.37 6.60 2693 2693_st1 hsa-asg-53 hsa-asg- 9.00 9.21 9.11 0.15 100 9.819.77 9.80 53_st2 hsa-asg- hsa-asg- 8.23 9.03 8.63 0.56 100 9.13 9.169.36 5032 5032_st2 hsa-asg- hsa-asg- 6.79 6.22 6.51 0.41 50 7.17 7.197.20 6892 6892_st2 hsa-asg- hsa-asg- 6.11 6.38 6.25 0.19 50 6.77 7.106.68 1911 1911_st1 hsa-asg- hsa-asg- 5.54 6.44 5.99 0.64 50 6.53 6.126.78 8675 8675_st1 hsa-asg-809 hsa-asg- 5.30 5.55 5.43 0.18 0 5.62 6.266.23 809_st2 hsa-asg- hsa-asg- 7.56 6.67 7.12 0.63 100 7.64 7.81 7.9211893 11893_st2 hsa-asg- hsa-asg- 7.69 6.65 7.17 0.74 100 7.67 8.01 7.841861 1861_st1 hsa-asg- hsa-asg- 5.37 5.46 5.42 0.06 50 6.46 5.93 6.576651 6651_st2 hsa-asg-471 hsa-asg- 7.27 7.54 7.41 0.19 0 8.03 8.17 8.19471_st1 hsa-asg- hsa-asg- 5.27 5.03 5.15 0.17 0 5.62 6.28 6.43 1086110861_st2 hsa-asg- hsa-asg- 7.88 7.63 7.75 0.18 100 8.30 8.52 8.63 43104310_st1 hsa-asg- hsa-asg- 7.18 7.33 7.25 0.10 100 7.40 8.27 8.68 71537153_st2 hsa-asg- hsa-asg- 4.64 5.21 4.93 0.40 0 5.60 5.75 5.95 68796879_st1 hsa-asg- hsa-asg- 5.07 5.38 5.22 0.22 0 5.89 6.31 5.98 73527352_st1 hsa-asg- hsa-asg- 6.60 6.65 6.63 0.04 100 7.41 7.98 7.34 1277512775_st1 hsa-asg- hsa-asg- 7.30 6.85 7.08 0.32 100 7.93 7.89 8.08 1361313613_st2 hsa-asg- hsa-asg- 1.61 2.23 1.92 0.43 0 3.11 2.31 2.82 97489748_st1 hsa-asg- hsa-asg- 6.36 5.77 6.06 0.42 50 6.71 6.93 6.77 76407640_st2 hsa-asg- hsa-asg- 5.94 6.20 6.07 0.19 0 6.96 6.55 6.82 1097110971_st2 hsa-asg- hsa-asg- 8.17 8.80 8.49 0.44 0 9.33 9.32 8.96 60926092_st2 hsa-asg-813 hsa-asg- 4.64 4.61 4.63 0.02 0 5.30 5.46 5.81813_st2 hsa-asg- hsa-asg- 6.78 6.74 6.76 0.03 100 7.41 7.55 7.76 64376437_st1 hsa-asg- hsa-asg- 7.38 7.47 7.43 0.06 100 8.25 8.49 8.30 1294612946_st1 hsa-asg- hsa-asg- 7.69 7.66 7.67 0.02 100 8.50 8.40 8.33 69486948_st2 hsa-asg- hsa-asg- 7.08 6.56 6.82 0.37 0 7.72 7.21 7.84 1148611486_st1 hsa-cand711 hsa- 9.10 9.13 9.11 0.02 50 9.89 9.84 9.99cand711_st1 hsa-cand588 hsa- 5.91 5.66 5.79 0.18 0 6.93 6.16 6.88cand588_st1 hsa-asg-555 hsa-asg- 6.78 5.89 6.34 0.63 50 6.58 7.15 7.10555_st1 hsa-asg- hsa-asg- 6.94 6.90 6.92 0.02 0 7.80 7.41 7.52 61496149_st2 hsa-asg- hsa-asg- 7.29 6.47 6.88 0.58 0 7.41 7.78 7.54 99209920_st1 hsa-asg- hsa-asg- 6.71 5.85 6.28 0.61 50 7.14 7.04 7.25 13911391_st1 hsa-asg- hsa-asg- 5.98 6.38 6.18 0.28 50 7.03 6.43 6.89 31503150_st1 hsa-asg- hsa-asg- 6.41 5.83 6.12 0.41 50 6.79 6.89 7.57 1382913829_st2 hsa-asg- hsa-asg- 6.09 5.71 5.90 0.27 0 5.98 6.73 6.64 93829382_st2 hsa-cand855 hsa- 5.68 6.13 5.91 0.32 0 6.18 6.83 6.90cand855_st1 hsa-asg- hsa-asg- 7.05 7.15 7.10 0.07 100 7.91 7.99 7.9610716 10716_st1 hsa-asg- hsa-asg- 7.96 7.83 7.90 0.10 100 8.49 9.03 8.8011698 11698_st2 hsa-cand902 hsa- 5.27 5.11 5.19 0.11 0 5.55 6.08 6.34cand902_st1 hsa-asg- hsa-asg- 8.37 8.10 8.24 0.19 100 9.23 9.50 9.1412919 12919_st2 hsa-asg- hsa-asg- 4.41 4.43 4.42 0.02 0 5.38 5.22 4.984261 4261_st1 hsa-asg- hsa-asg- 6.19 6.41 6.30 0.15 50 7.28 7.20 7.201153 1153_st1 hsa-asg- hsa-asg- 6.00 5.01 5.51 0.70 0 6.37 6.18 6.723841 3841_st2 hsa-asg- hsa-asg- 10.83 10.20 10.52 0.45 100 10.96 11.7711.58 3231 3231_st2 hsa-asg-225 hsa-asg- 4.85 4.26 4.56 0.41 0 5.27 5.145.09 225_st1 hsa-asg- hsa-asg- 7.26 6.73 6.99 0.37 100 7.74 7.52 7.952017 2017_st2 hsa-asg- hsa-asg- 6.48 6.32 6.40 0.11 100 7.20 6.99 7.156623 6623_st2 hsa-asg- hsa-asg- 4.94 4.87 4.91 0.05 0 5.69 5.85 5.529526 9526_st2 hsa-asg- hsa-asg- 4.99 4.92 4.95 0.05 0 5.41 6.03 6.503014 3014_st2 hsa-asg- hsa-asg- 6.97 7.01 6.99 0.03 100 7.21 8.21 8.4812957 12957_st1 hsa-asg- hsa-asg- 4.05 3.55 3.80 0.36 0 4.67 5.06 5.112564 2564_st2 hsa-asg- hsa-asg- 4.34 5.23 4.79 0.63 0 5.80 5.88 5.7010473 10473_st1 hsa-asg- hsa-asg- 2.58 2.99 2.79 0.29 0 3.30 3.79 3.088303 8303_st2 hsa-asg- hsa-asg- 5.03 5.55 5.29 0.37 50 6.06 6.48 6.9111310 11310_st2 hsa-asg- hsa-asg- 5.91 5.79 5.85 0.08 0 6.63 7.49 6.516831 6831_st2 hsa-asg- hsa-asg- 7.15 6.72 6.94 0.30 0 7.19 8.37 8.5011373 11373_st1 hsa-asg- hsa-asg- 6.00 6.18 6.09 0.12 0 6.80 6.90 7.496005 6005_st1 hsa-asg- hsa-asg- 8.41 9.02 8.72 0.43 0 9.52 9.57 9.594654 4654_st1 hsa-asg- hsa-asg- 7.73 7.35 7.54 0.27 100 7.94 8.32 9.269403 9403_st2 hsa-asg- hsa-asg- 7.75 7.64 7.70 0.07 100 8.93 8.38 8.317893 7893_st2 hsa-asg- hsa-asg- 7.48 6.81 7.15 0.48 100 7.51 8.29 8.6213000 13000_st1 hsa-asg- hsa-asg- 6.00 5.86 5.93 0.10 50 6.32 7.51 7.2310883 10883_st1 hsa-asg- hsa-asg- 4.75 5.38 5.06 0.44 0 5.89 5.83 5.931689 1689_st2 hsa-asg- hsa-asg- 6.57 5.59 6.08 0.70 50 6.49 7.32 7.529412 9412_st2 hsa-asg- hsa-asg- 7.97 7.34 7.66 0.45 100 8.57 8.51 8.533271 3271_st1 hsa-asg- hsa-asg- 6.71 5.95 6.33 0.54 50 7.40 7.21 7.7513559 13559_st2 hsa-asg- hsa-asg- 3.80 3.65 3.73 0.11 0 4.26 4.51 4.5811898 11898_st2 hsa-asg- hsa-asg- 3.31 3.55 3.43 0.17 0 4.08 4.39 4.323081 3081_st1 hsa-asg- hsa-asg- 6.19 5.25 5.72 0.67 0 6.66 6.68 6.902858 2858_st1 hsa-asg-278 hsa-asg- 4.64 5.31 4.97 0.47 0 5.07 6.30 5.91278_st1 hsa-asg- hsa-asg- 7.67 7.92 7.80 0.18 100 8.63 8.64 8.76 54235423_st2 hsa-asg- hsa-asg- 4.27 4.67 4.47 0.28 0 5.27 5.56 5.85 92579257_st2 hsa-asg- hsa-asg- 6.15 5.71 5.93 0.31 50 6.44 7.32 7.10 1019710197_st2 hsa-asg- hsa-asg- 7.16 6.81 6.98 0.25 100 8.52 8.24 8.04 65076507_st2 hsa-asg- hsa-asg- 5.84 5.11 5.48 0.51 0 6.52 6.59 6.91 90159015_st1 hsa-asg- hsa-asg- 1.72 1.98 1.85 0.18 0 3.11 2.69 3.38 54605460_st2 hsa-asg- hsa-asg- 8.95 9.11 9.03 0.12 100 10.96 10.10 9.6513308 13308_st2 hsa-asg- hsa-asg- 4.27 4.99 4.63 0.50 0 5.93 5.59 5.834440 4440_st1 hsa-asg- hsa-asg- 6.41 5.52 5.97 0.63 100 7.01 7.15 7.051375 1375_st1 hsa-asg- hsa-asg- 6.17 4.96 5.57 0.85 0 6.64 6.43 6.998763 8763_st2 hsa-asg- hsa-asg- 8.47 8.68 8.58 0.15 100 9.33 9.90 10.512872 2872_st2 hsa-asg- hsa-asg- 6.63 6.63 6.63 0.00 100 7.45 8.42 8.2012460 12460_st2 hsa-asg- hsa-asg- 5.03 5.43 5.23 0.28 0 6.18 5.74 6.728138 8138_st2 hsa-asg- hsa-asg- 9.09 9.11 9.10 0.01 100 11.02 10.13 9.945304 5304_st1 hsa-asg- hsa-asg- 5.89 4.49 5.19 0.99 0 6.20 6.03 6.454971 4971_st1 hsa-asg- hsa-asg- 5.91 6.38 6.15 0.33 50 7.20 7.06 7.399343 9343_st1 hsa-asg- hsa-asg- 5.07 5.17 5.12 0.07 0 6.02 6.24 6.388271 8271_st2 hsa-asg- hsa-asg- 7.43 6.67 7.05 0.54 100 8.64 8.29 8.5613260 13260_st1 hsa-asg- hsa-asg- 4.34 4.23 4.29 0.08 0 5.41 5.90 4.5512105 12105_st2 hsa-asg- hsa-asg- 3.20 2.42 2.81 0.55 0 3.56 4.97 3.887685 7685_st2 hsa-asg- hsa-asg- 5.79 5.55 5.67 0.17 0 7.37 6.62 6.683360 3360_st2 hsa-asg- hsa-asg- 5.37 4.19 4.78 0.83 0 5.36 6.42 6.505576 5576_st1 hsa-asg- hsa-asg- 5.27 4.04 4.66 0.87 0 5.49 6.28 5.854843 4843_st2 hsa-asg- hsa-asg- 5.27 4.49 4.88 0.55 0 5.71 6.22 7.157009 7009_st1 hsa-asg- hsa-asg- 3.08 4.40 3.74 0.93 0 4.76 5.14 5.478928 8928_st2 hsa-asg- hsa-asg- 3.97 4.72 4.35 0.53 0 5.07 5.49 5.5812777 12777_st2 hsa-asg- hsa-asg- 5.23 4.61 4.92 0.44 0 5.71 6.55 7.097069 7069_st1 hsa-cand965 hsa- 6.64 5.19 5.92 1.02 0 7.25 7.94 7.66cand965_st1 hsa-asg- hsa-asg- 5.27 4.08 4.67 0.84 0 5.80 6.12 6.29 1369313693_st2 hsa-asg- hsa-asg- 5.03 4.26 4.65 0.54 0 6.06 6.18 6.03 83298329_st1 hsa-asg- hsa-asg- 5.65 4.96 5.31 0.49 0 5.89 6.67 6.69 35973597_st2 hsa-asg- hsa-asg- 5.30 3.92 4.61 0.98 0 5.55 6.43 6.33 98529852_st1 hsa-asg- hsa-asg- 3.31 2.61 2.96 0.49 0 4.53 4.39 3.97 31073107_st1 hsa-asg- hsa-asg- 10.81 10.39 10.60 0.30 100 12.93 11.30 12.1114245 14245_st1 hsa-asg- hsa-asg- 5.07 4.08 4.58 0.70 0 5.85 6.08 6.316949 6949_st1 hsa-asg- hsa-asg- 4.41 3.65 4.03 0.54 0 5.64 5.29 5.497319 7319_st2 hsa-asg- hsa-asg- 5.34 4.23 4.78 0.78 0 6.75 5.82 6.669022 9022_st2 hsa-asg- hsa-asg- 4.80 4.89 4.85 0.07 0 6.58 5.93 6.3912246 12246_st2 hsa-asg- hsa-asg- 5.84 5.91 5.87 0.05 0 6.44 8.01 8.098917 8917_st2 hsa-asg- hsa-asg- 4.94 5.55 5.25 0.43 0 7.74 7.02 7.0913244 13244_st1 hsa-cand345 hsa- 4.75 4.23 4.49 0.37 0 6.31 5.64 5.72cand345_st1 hsa-asg- hsa-asg- 4.99 6.40 5.69 1.00 50 7.42 7.02 8.5712702 12702_st1 hsa-asg- hsa-asg- 5.68 4.67 5.17 0.72 0 7.14 7.01 6.7313230 13230_st2 hsa-asg- hsa-asg- 4.59 5.70 5.14 0.79 50 7.71 7.34 6.6112902 12902_st1 hsa-asg- hsa-asg- 6.00 4.92 5.46 0.77 0 7.44 6.96 7.2613237 13237_st1 hsa-asg- hsa-asg- 5.54 5.21 5.37 0.23 0 5.85 7.63 7.944002 4002_st1 hsa-asg-21 hsa-asg- 5.11 3.45 4.28 1.18 0 5.67 5.83 6.8921_st2 hsa-asg- hsa-asg- 6.21 5.46 5.84 0.53 0 7.38 7.72 8.38 1014910149_st1 hsa-asg- hsa-asg- 6.39 3.88 5.14 1.78 50 6.51 7.95 6.78 53035303_st1 hsa-asg- hsa-asg- 5.47 3.34 4.41 1.51 0 6.16 6.85 7.05 1066610666_st1 hsa-asg-335 hsa-asg- 5.86 6.02 5.94 0.11 50 6.63 8.54 8.65335_st1 hsa-asg- hsa-asg- 3.97 4.49 4.23 0.37 0 5.38 6.36 6.71 62506250_st2 hsa-asg- hsa-asg- 4.27 3.92 4.10 0.25 0 5.44 6.38 6.78 82088208_st1 hsa-asg- hsa-asg- 5.65 5.83 5.74 0.13 50 6.32 8.35 8.75 1038710387_st1 hsa-asg- hsa-asg- 7.17 7.07 7.12 0.07 100 9.94 8.87 8.61 1324813248_st1 hsa-asg- hsa-asg- 4.34 2.74 3.54 1.13 0 4.48 6.12 6.27 1207912079_st1 hsa-asg- hsa-asg- 5.91 4.96 5.44 0.67 50 7.15 7.23 7.47 45574557_st2 hsa-asg- hsa-asg- 8.00 5.49 6.75 1.78 50 8.27 9.59 9.28 1393713937_st1 hsa-miR165 hsa- 4.20 2.55 3.38 1.17 0 4.80 5.14 5.47miR165_st1 hsa-asg- hsa-asg- 4.80 4.00 4.40 0.56 0 4.92 7.43 7.55 86828682_st2 hsa-asg- hsa-asg- 4.20 2.68 3.44 1.08 0 5.07 5.95 6.52 64136413_st2 hsa-asg- hsa-asg- 5.07 3.55 4.31 1.08 0 6.15 6.40 7.12 1330413304_st2 hsa-asg- hsa-asg- 2.84 4.43 3.63 1.13 0 4.37 6.59 6.40 42054205_st2 hsa-asg-870 hsa-asg- 3.97 3.39 3.68 0.41 0 4.37 6.85 7.49870_st1 hsa-asg- hsa-asg- 8.77 6.85 7.81 1.35 50 9.87 10.31 10.51 70747074_st1 hsa-asg- hsa-asg- 6.43 3.45 4.94 2.11 50 8.09 7.42 7.28 1327913279_st1 hsa-miR- hsa-asg- 10.04 9.23 9.64 0.57 100 11.33 11.04 10.7329b 14229_st2 hsa-miR-9 hsa- 4.41 7.16 5.79 1.95 50 3.01 2.94 3.88cand90_st2 hsa-miR- hsa-asg- 8.45 8.63 8.54 0.12 100 7.10 6.48 6.07 4215033_st2 MEL vs LNneg Micro LNneg Log2 RNA 4 5 6 7 Mean SD % ttest DiffFC hsa-miR- 4.15 3.26 5.06 4.23 4.30 0.67 0 5.46E−06 8.04 262.3 205hsa-miR- 5.94 5.88 6.58 6.38 5.84 0.71 29 6.15E−06 7.28 155.8 203hsa-miR- 4.64 5.22 4.00 3.05 4.07 1.18 0 1.75E−04 6.38 83.3 211 hsa-miR-5.45 7.04 6.25 6.22 6.44 0.84 86 6.84E−05 5.95 61.8 509 hsa-miR- 5.215.40 5.47 5.75 5.35 1.22 14 6.16E−04 5.93 60.8 513 hsa-miR- 8.69 6.857.87 7.98 7.80 0.79 100 1.26E−03 3.83 14.3 200c hsa-miR- 7.38 5.36 4.765.89 5.56 1.13 0 3.55E−03 3.63 12.4 132 hsa-miR- 6.01 6.77 6.66 6.086.48 0.41 100 4.74E−04 3.58 12.0 506 hsa-miR- 6.49 6.65 6.49 6.15 6.610.47 100 1.78E−04 3.48 11.2 508 hsa-miR- 6.94 6.34 6.12 6.04 6.31 0.5186 7.09E−04 3.27 9.7 183 hsa-miR- 7.11 6.78 7.40 8.10 6.95 0.67 1003.27E−03 3.13 8.8 200b hsa-miR- 4.88 5.04 4.33 4.66 5.03 0.60 142.46E−03 3.12 8.7 510 hsa-miR- 2.52 3.03 2.75 2.72 2.81 0.40 0 2.96E−033.09 8.5 561 hsa-miR- 4.75 3.77 5.49 5.40 4.29 0.91 29 2.63E−03 3.09 8.5493-5p hsa-miR- 2.91 3.03 2.98 3.05 2.99 0.07 0 6.98E−06 3.04 8.2 514hsa-miR- 5.12 5.71 5.61 6.12 5.47 0.42 14 3.38E−05 2.98 7.9 584hsa-miR-9 5.25 3.86 4.00 4.78 4.18 0.62 14 4.02E−03 2.77 6.8 hsa-miR-3.18 2.91 2.28 3.20 2.87 0.39 0 3.56E−03 2.60 6.1 576 hsa-miR-9* 7.585.14 5.78 6.50 5.89 0.88 43 2.76E−02 2.48 5.6 hsa-miR-96 4.18 3.86 3.764.18 3.87 0.61 0 2.76E−03 2.38 5.2 hsa-miR- 9.24 8.03 8.90 8.80 8.620.49 100 2.90E−03 2.30 4.9 182 hsa-miR- 3.05 2.34 3.40 3.31 3.08 0.38 01.41E−03 1.93 3.8 605 hsa-miR- 8.46 7.14 7.29 8.29 7.45 0.76 1001.76E−02 1.74 3.3 421 hsa-miR- 4.51 5.16 5.06 4.86 4.42 0.86 0 4.10E−021.64 3.1 432 hsa-miR- 3.00 2.66 2.86 3.62 2.98 0.33 0 3.85E−02 1.57 3.0429 hsa-miR- 3.35 3.26 2.86 3.42 3.08 0.30 0 2.84E−04 1.49 2.8 592hsa-miR- 5.04 4.92 5.44 5.38 4.88 0.47 29 1.71E−02 1.29 2.5 495 hsa-miR-4.42 3.94 3.49 4.15 3.68 0.62 0 3.21E−02 1.27 2.4 187 hsa-miR- 7.03 6.426.79 7.17 6.52 0.48 57 1.01E−02 1.26 2.4 382 hsa-miR- 7.89 7.98 8.368.64 8.10 0.31 100 5.13E−03 1.21 2.3 34a hsa-miR- 4.39 4.88 5.02 5.054.66 0.37 0 1.05E−02 1.11 2.2 376a hsa-miR- 7.68 8.30 8.26 8.19 8.010.38 71 6.72E−03 1.09 2.1 193b hsa-miR- 9.83 9.79 9.82 11.16 10.27 0.51100 3.45E−02 1.02 2.0 222 hsa-miR- 4.79 5.40 4.61 5.00 4.87 0.31 04.39E−03 0.99 2.0 365 hsa-miR- 5.51 6.04 6.23 6.24 6.21 0.41 1002.94E−02 0.96 1.9 494 hsa-miR- 11.39 11.23 11.48 11.78 11.37 0.29 1002.07E−02 0.66 1.6 27a hsa-miR- 6.24 6.90 6.33 6.60 6.35 0.32 86 3.09E−020.65 1.6 379 hsa-miR- 12.20 12.27 12.17 12.32 12.15 0.12 100 1.14E−020.62 1.5 27b hsa-miR-21 13.29 13.27 13.26 13.27 13.30 0.17 100 7.85E−030.48 1.4 hsa-miR-24 13.12 13.42 13.39 13.35 13.21 0.25 100 3.90E−02 0.471.4 hsa-miR- 13.46 13.32 13.05 13.12 13.24 0.15 100 5.58E−03 0.44 1.423b hsa-miR- 12.42 12.40 12.48 12.91 12.61 0.20 100 3.53E−02 0.44 1.4221 hsa-let-7e 12.26 12.38 12.02 12.29 12.20 0.14 100 1.19E−02 0.42 1.3hsa-miR- 11.57 11.39 11.43 11.69 11.55 0.15 100 9.67E−03 0.41 1.3 30bhsa-miR- 4.66 5.14 4.98 5.00 4.93 0.19 14 4.31E−02 0.35 1.3 618 hsa-miR-13.78 13.78 13.43 13.49 13.62 0.15 100 2.11E−02 0.33 1.3 23a hsa-miR-12.71 13.05 12.71 12.78 12.84 0.18 100 3.48E−02 −0.37 1.3 199a* hsa-miR-13.38 13.41 13.00 12.96 13.18 0.19 100 1.06E−02 −0.70 1.6 145 hsa-miR-11.70 12.21 11.60 12.05 11.67 0.40 100 4.88E−02 −0.71 1.6 99a hsa-miR-12.94 13.05 12.65 12.85 12.86 0.15 100 1.94E−02 −0.81 1.8 195 hsa-miR-7.09 7.44 7.43 8.10 7.41 0.44 100 3.15E−02 −0.93 1.9 331 hsa-miR- 12.6813.13 12.70 12.76 12.68 0.33 100 9.51E−03 −1.05 2.1 143 hsa-miR- 10.1010.47 10.32 10.53 10.17 0.33 100 1.43E−02 −1.15 2.2 199a hsa-miR- 12.3812.55 11.96 12.37 12.30 0.19 100 2.23E−03 −1.20 2.3 10b hsa-miR- 5.866.72 6.94 7.16 6.69 0.44 86 6.99E−03 −1.25 2.4 339 hsa-miR-7 9.57 9.1510.45 10.74 9.84 0.64 100 2.62E−02 −1.35 2.5 hsa-miR- 12.16 11.36 11.9411.75 11.76 0.28 100 3.17E−04 −1.39 2.6 148a hsa-miR- 10.99 10.46 10.3311.06 10.85 0.34 100 3.80E−03 −1.42 2.7 29b hsa-miR- 13.30 13.59 13.5413.49 13.55 0.19 100 7.65E−03 −1.44 2.7 342 hsa-miR- 7.25 7.91 7.69 7.887.61 0.28 57 2.19E−03 −1.55 2.9 497 hsa-miR- 6.94 6.44 7.29 8.01 6.970.57 100 7.98E−03 −1.58 3.0 215 hsa-miR- 10.21 10.04 11.30 11.59 10.590.61 100 2.13E−02 −1.59 3.0 192 hsa-miR-17- 6.07 6.07 7.18 7.83 6.800.63 86 6.29E−03 −1.80 3.5 3p hsa-miR-95 9.72 8.44 9.12 10.06 9.02 0.64100 4.05E−02 −1.83 3.5 hsa-miR- 9.11 8.75 8.76 9.53 8.86 0.39 1001.53E−03 −1.85 3.6 505 hsa-miR- 6.71 6.13 7.22 7.99 7.11 0.70 869.43E−03 −1.95 3.9 598 hsa-miR- 9.85 9.82 10.27 10.95 10.42 0.51 1001.20E−03 −1.99 4.0 29c hsa-miR- 5.97 6.11 5.66 5.24 5.27 0.81 141.91E−02 −2.11 4.3 133b hsa-miR- 6.62 7.16 5.90 6.30 6.19 0.63 715.46E−03 −2.23 4.7 133a hsa-miR-1 10.70 10.19 9.58 10.00 9.58 0.84 1003.32E−03 −3.07 8.4 hsa-asg- 2.95 3.48 3.59 3.49 3.56 0.77 0 2.06E−056.71 104.3 5638 hsa-asg- 3.70 4.47 3.68 4.15 3.96 0.36 0 1.13E−04 4.3420.3 11338 hsa-asg- 3.70 2.91 4.51 3.49 4.04 1.22 14 6.21E−03 4.10 17.25630 hsa-asg- 2.81 2.78 2.63 2.47 2.82 0.34 0 1.66E−03 2.91 7.5 13997hsa-asg- 3.27 3.68 2.28 2.72 2.97 0.44 0 1.45E−03 2.77 6.8 8428 hsa-asg-5.21 5.12 5.06 5.21 5.18 0.53 0 3.29E−04 2.64 6.2 9186 hsa-asg- 3.232.91 3.19 2.97 3.01 0.22 0 5.93E−04 2.57 5.9 1296 hsa-asg- 2.52 3.152.98 2.81 2.87 0.34 0 5.24E−05 2.52 5.7 9723 hsa-asg- 3.09 3.37 3.302.81 3.09 0.34 0 1.05E−03 2.23 4.7 11380 hsa-asg- 3.14 3.09 3.19 4.003.29 0.33 0 2.75E−03 2.10 4.3 2261 hsa-asg- 6.03 7.32 6.38 5.77 6.480.67 100 6.21E−03 2.10 4.3 1519 hsa-asg- 3.18 3.37 2.40 2.30 3.08 0.62 07.11E−03 2.09 4.3 1794 hsa-asg- 4.32 4.17 4.90 4.97 4.55 0.29 0 9.72E−052.03 4.1 12271 hsa-asg- 4.21 3.98 5.14 5.46 4.38 0.67 0 1.68E−02 2.034.1 14208 hsa-asg- 4.29 4.25 3.49 3.86 3.78 0.40 0 1.35E−02 1.93 3.88486 hsa-asg- 8.38 8.23 8.84 9.13 8.70 0.44 14 7.16E−04 1.90 3.7 5640hsa-cand317 3.88 3.48 4.61 4.91 3.90 0.74 14 4.34E−02 1.71 3.3 hsa-asg-3.52 3.15 2.63 3.56 3.32 0.41 0 2.08E−03 1.71 3.3 2335 hsa-asg- 2.223.03 3.09 2.81 2.96 0.37 0 1.77E−02 1.70 3.3 1587 hsa-asg- 4.09 5.412.86 5.11 4.34 0.86 0 4.25E−02 1.63 3.1 4803 hsa-asg- 9.37 8.64 9.1110.21 9.28 0.60 43 9.15E−03 1.59 3.0 13278 hsa-asg-497 5.64 6.69 5.315.62 5.78 0.43 57 2.03E−03 1.55 2.9 hsa-asg- 4.15 4.47 3.09 5.08 4.160.81 0 4.31E−02 1.52 2.9 9796 hsa-asg- 3.48 4.14 3.49 4.89 3.97 0.60 03.98E−02 1.50 2.8 10031 hsa-asg- 4.73 3.90 4.07 4.83 4.28 0.41 02.10E−03 1.50 2.8 9980 hsa-asg- 4.51 4.53 4.07 4.79 4.42 0.23 0 1.29E−021.49 2.8 12453 hsa-asg- 5.52 5.81 5.28 4.81 5.25 0.38 0 2.53E−02 1.462.7 11489 hsa-asg- 3.81 3.48 3.49 3.28 3.36 0.28 0 2.61E−04 1.45 2.711361 hsa-asg- 6.29 5.45 6.12 5.04 5.49 0.52 29 8.72E−03 1.43 2.7 12807hsa-asg- 6.14 4.31 5.14 4.71 4.92 0.59 14 1.76E−02 1.38 2.6 7511hsa-asg- 4.82 4.44 4.33 4.67 4.72 0.26 0 1.41E−02 1.35 2.5 9762 hsa-asg-6.82 6.39 5.78 6.93 6.40 0.52 0 1.63E−02 1.29 2.4 12372 hsa-asg- 7.737.15 7.34 8.45 7.66 0.64 43 3.47E−02 1.29 2.4 4223 hsa-asg- 5.93 5.014.71 5.08 5.41 0.48 43 1.60E−02 1.24 2.4 11402 hsa-asg- 6.60 6.15 5.966.66 6.38 0.25 71 4.50E−04 1.24 2.4 8633 hsa-asg- 5.05 6.19 5.61 5.745.71 0.44 0 1.11E−02 1.21 2.3 4780 hsa-asg- 6.41 5.66 6.18 5.47 5.830.35 0 3.21E−03 1.15 2.2 9117 hsa-asg- 4.62 5.36 4.71 4.44 4.60 0.44 141.97E−02 1.14 2.2 1362 hsa-asg- 9.36 9.85 9.37 9.86 9.44 0.42 1009.27E−03 1.13 2.2 14233 hsa-asg- 12.13 11.26 11.31 11.44 11.70 0.45 1001.89E−02 1.10 2.1 13309 hsa-asg- 7.28 6.38 5.94 6.86 6.65 0.42 713.49E−02 1.10 2.1 13841 hsa-asg- 6.27 7.18 6.38 5.73 6.23 0.48 431.83E−02 1.10 2.1 14001 hsa-asg- 3.97 4.47 5.17 4.00 4.59 0.49 02.24E−02 1.06 2.1 12320 hsa-asg- 6.47 6.61 6.00 7.06 6.55 0.33 1001.63E−02 1.05 2.1 9227 hsa-asg- 5.79 6.11 6.05 6.36 6.12 0.21 434.61E−04 1.04 2.1 7922 hsa-asg- 8.69 8.11 8.21 8.21 8.25 0.29 1002.10E−03 1.02 2.0 9261 hsa-cand732 7.38 6.50 6.81 7.13 6.80 0.39 571.66E−02 1.02 2.0 hsa-asg- 5.18 5.18 4.94 4.81 5.03 0.21 0 3.23E−04 1.022.0 13924 hsa-asg- 4.64 4.10 4.14 5.59 4.63 0.55 0 4.52E−02 1.02 2.02889 hsa-asg- 6.53 6.15 5.83 6.42 6.10 0.30 29 5.93E−03 1.02 2.0 12381hsa-asg- 6.98 6.39 7.03 5.92 6.54 0.39 86 1.15E−02 1.02 2.0 8003hsa-asg- 5.08 5.08 5.31 5.34 5.06 0.26 0 6.75E−03 1.01 2.0 7262 hsa-asg-10.26 10.37 9.46 10.36 10.02 0.35 100 4.04E−02 0.99 2.0 11850 hsa-asg-6.01 5.27 5.81 5.74 5.76 0.24 0 1.22E−03 0.98 2.0 7027 hsa-asg- 7.967.77 7.71 8.37 7.87 0.30 71 6.10E−03 0.97 2.0 12937 hsa-asg-345 4.584.44 4.76 4.56 4.71 0.19 0 3.29E−04 0.97 2.0 hsa-asg- 5.46 5.40 4.985.11 5.42 0.44 43 2.66E−02 0.96 1.9 4969 hsa-asg- 5.65 5.73 4.98 5.265.44 0.30 0 1.79E−02 0.96 1.9 3798 hsa-asg- 6.56 6.00 6.94 5.95 6.200.40 43 1.88E−02 0.95 1.9 4703 hsa-asg- 3.14 3.32 3.30 3.46 3.12 0.45 04.96E−02 0.95 1.9 8792 hsa-asg- 5.35 4.97 5.06 4.60 5.05 0.38 0 1.27E−020.94 1.9 4460 hsa-asg- 7.24 7.11 6.96 7.43 7.12 0.29 0 5.18E−03 0.94 1.94026 hsa-cand421 10.62 10.83 10.39 10.88 10.62 0.26 100 1.61E−02 0.931.9 hsa-asg- 7.79 7.34 7.21 7.93 7.59 0.41 100 1.88E−02 0.93 1.9 3670hsa-asg- 7.49 7.14 7.29 7.16 7.22 0.16 100 1.22E−03 0.88 1.8 9130hsa-asg- 5.40 5.54 5.28 4.95 5.43 0.28 29 4.00E−02 0.88 1.8 7783hsa-asg- 5.22 5.81 5.02 4.94 5.27 0.39 43 3.35E−02 0.86 1.8 3944hsa-asg- 5.00 5.40 4.94 4.60 4.93 0.30 0 6.39E−03 0.86 1.8 13798hsa-asg- 8.33 8.04 8.15 8.43 8.11 0.31 100 8.28E−03 0.86 1.8 6870hsa-asg- 4.93 5.14 5.37 4.67 4.85 0.37 14 2.70E−02 0.85 1.8 2460hsa-asg- 4.18 4.17 4.21 4.81 4.43 0.28 14 3.48E−02 0.84 1.8 11267hsa-asg- 5.49 6.08 5.31 5.38 5.46 0.29 43 8.12E−03 0.83 1.8 3604hsa-asg- 5.86 6.47 6.47 5.82 6.05 0.30 43 9.81E−03 0.80 1.7 4296hsa-asg- 8.80 8.21 7.77 8.07 8.19 0.35 100 4.51E−02 0.76 1.7 8866hsa-asg- 4.97 5.14 4.56 4.81 4.88 0.21 100 5.92E−03 0.73 1.7 12471hsa-asg- 7.39 7.30 7.70 7.93 7.49 0.31 100 3.18E−02 0.72 1.7 11515hsa-asg- 5.59 6.03 5.92 5.52 5.80 0.26 71 1.70E−02 0.71 1.6 7252hsa-asg- 6.02 5.16 5.83 5.67 5.82 0.38 57 4.23E−02 0.70 1.6 11458hsa-asg- 9.03 8.98 8.45 8.74 8.71 0.28 0 1.98E−02 0.69 1.6 13453hsa-asg- 9.58 9.30 9.81 9.23 9.35 0.25 100 9.03E−03 0.68 1.6 3722hsa-asg- 10.93 10.94 10.45 11.11 10.84 0.20 100 3.58E−02 0.67 1.6 3992hsa-asg- 6.49 6.57 5.96 6.12 6.34 0.23 14 1.39E−02 0.66 1.6 4373hsa-asg- 7.22 7.00 6.92 7.50 7.08 0.21 100 4.17E−03 0.66 1.6 9480hsa-asg- 12.68 12.56 12.44 12.69 12.59 0.12 100 9.16E−03 0.65 1.6 7939hsa-asg- 5.69 6.54 6.30 6.25 6.22 0.28 100 1.86E−02 0.64 1.6 4448hsa-asg- 6.42 5.81 5.94 6.19 6.20 0.26 71 2.18E−02 0.64 1.6 1338hsa-asg- 8.09 7.62 7.25 7.70 7.53 0.30 100 2.54E−02 0.63 1.5 11617hsa-asg-954 9.66 9.68 9.05 9.09 9.31 0.26 100 1.62E−02 0.62 1.5 hsa-asg-5.85 6.15 5.63 6.58 6.01 0.33 86 4.50E−02 0.62 1.5 2381 hsa-asg- 4.985.45 5.34 5.39 5.35 0.30 14 2.72E−02 0.62 1.5 2697 hsa-asg- 5.35 5.755.83 5.70 5.66 0.18 0 3.79E−03 0.59 1.5 2365 hsa-asg- 5.21 5.20 5.214.89 5.09 0.12 0 7.55E−03 0.59 1.5 9795 hsa-asg- 5.25 5.22 4.90 5.214.94 0.30 14 4.64E−02 0.59 1.5 12457 hsa-asg- 6.02 6.21 5.47 5.40 5.770.31 14 3.91E−02 0.58 1.5 2500 hsa-asg- 6.84 6.79 6.76 6.83 6.84 0.12100 3.51E−02 0.56 1.5 8882 hsa-asg- 5.65 5.64 5.34 5.42 5.37 0.22 571.38E−02 0.55 1.5 5973 hsa-asg- 5.82 6.23 5.96 5.99 6.07 0.26 863.62E−02 0.54 1.5 6616 hsa-asg- 6.47 6.12 6.14 6.22 6.29 0.14 862.95E−03 0.53 1.4 13294 hsa-asg- 5.16 4.92 5.14 5.19 4.96 0.29 574.22E−02 0.53 1.4 9521 hsa-asg- 8.92 8.46 8.57 8.69 8.56 0.21 1001.63E−02 0.52 1.4 7450 hsa-asg- 4.39 5.01 4.61 4.40 4.53 0.23 142.83E−02 0.51 1.4 4030 hsa-asg- 7.43 6.96 6.86 6.90 6.98 0.25 1004.49E−02 0.50 1.4 12406 hsa-asg- 5.65 5.46 5.52 5.53 5.64 0.15 574.11E−03 0.48 1.4 12885 hsa-cand873 5.54 5.88 5.47 5.33 5.42 0.26 434.38E−02 0.48 1.4 hsa-asg- 13.31 13.25 13.23 13.29 13.30 0.06 1006.79E−03 0.47 1.4 14201 hsa-asg- 10.47 10.19 10.43 10.26 10.32 0.18 1004.15E−02 0.46 1.4 8568 hsa-asg- 8.01 7.49 7.88 7.61 7.68 0.19 573.96E−02 0.45 1.4 3239 hsa-asg- 7.39 7.25 7.18 7.03 7.17 0.13 1002.58E−03 0.45 1.4 4984 hsa-asg- 5.77 5.99 5.71 5.62 5.79 0.15 577.62E−03 0.44 1.4 2093 hsa-asg-733 5.95 6.06 5.92 5.70 5.96 0.18 1001.61E−02 0.43 1.3 hsa-asg-89 6.20 6.37 6.20 5.86 6.15 0.17 100 1.60E−020.40 1.3 hsa-asg- 5.80 5.83 5.74 5.49 5.73 0.15 100 1.12E−02 0.39 1.312816 hsa-asg- 5.21 5.49 5.44 5.44 5.45 0.18 0 2.11E−02 0.39 1.3 11057hsa-asg- 6.34 6.35 6.08 6.07 6.20 0.12 100 9.20E−03 0.36 1.3 6305hsa-asg- 5.52 5.64 5.58 5.33 5.59 0.19 14 3.93E−02 0.36 1.3 4587hsa-asg- 6.02 5.83 5.96 5.84 5.92 0.08 100 4.34E−02 0.16 1.1 2483hsa-asg-758 6.66 6.55 6.28 6.54 6.51 0.16 100 4.22E−02 −0.29 1.2hsa-asg- 6.55 6.54 6.75 6.65 6.61 0.14 100 2.89E−02 −0.30 1.2 7410hsa-asg- 10.56 10.07 10.35 10.32 10.30 0.16 100 3.48E−02 −0.33 1.3 2899hsa-asg- 9.43 9.22 9.31 9.60 9.33 0.17 29 4.56E−02 −0.35 1.3 7628hsa-asg- 10.85 10.63 10.84 10.82 10.77 0.15 100 1.74E−02 −0.35 1.3 14034hsa-asg- 10.07 9.70 10.10 10.17 9.91 0.20 100 4.83E−02 −0.37 1.3 8253hsa-asg- 4.97 5.14 4.90 4.97 4.95 0.17 14 2.20E−02 −0.38 1.3 4426hsa-asg- 7.04 6.74 6.66 6.77 6.75 0.16 100 3.73E−02 −0.38 1.3 8655hsa-asg- 11.69 11.27 11.38 11.58 11.45 0.15 100 1.44E−02 −0.39 1.3 10632hsa-asg-974 6.87 6.87 6.86 7.24 6.99 0.22 100 4.65E−02 −0.39 1.3hsa-asg- 6.59 6.29 6.27 6.81 6.48 0.20 29 3.65E−02 −0.39 1.3 7218hsa-asg-206 9.93 9.51 9.64 9.98 9.76 0.16 100 3.84E−02 −0.40 1.3hsa-asg- 8.12 7.98 7.84 7.71 7.88 0.21 100 3.39E−02 −0.41 1.3 3565hsa-cand559 7.23 7.44 7.52 7.40 7.39 0.19 100 2.71E−02 −0.41 1.3hsa-asg- 8.43 8.28 8.16 8.65 8.40 0.23 100 4.36E−02 −0.42 1.3 1809hsa-asg- 9.02 8.49 8.85 8.86 8.74 0.18 100 1.81E−02 −0.42 1.3 4020hsa-asg- 8.42 8.16 8.21 8.48 8.27 0.17 100 1.56E−02 −0.42 1.3 10392hsa-asg- 6.63 6.35 6.50 6.82 6.53 0.17 43 1.43E−02 −0.42 1.3 9501hsa-asg-976 6.74 6.94 6.41 6.63 6.65 0.23 100 4.09E−02 −0.43 1.3hsa-asg- 10.13 9.79 9.88 9.88 9.92 0.11 100 1.15E−02 −0.43 1.3 5749hsa-asg- 9.42 9.34 9.11 9.38 9.28 0.15 100 1.85E−02 −0.43 1.4 8631hsa-asg- 9.57 9.28 9.24 9.59 9.34 0.17 14 1.23E−02 −0.44 1.4 7051hsa-asg- 6.02 6.00 6.23 6.30 6.08 0.17 57 4.92E−02 −0.45 1.4 10506hsa-asg-507 3.27 2.78 3.19 3.28 3.05 0.21 0 2.51E−02 −0.45 1.4 hsa-asg-8.67 8.16 8.33 8.40 8.26 0.23 100 3.98E−02 −0.45 1.4 6018 hsa-asg- 9.729.42 9.46 9.83 9.57 0.18 57 1.33E−02 −0.46 1.4 3240 hsa-asg-504 9.058.87 8.78 8.93 8.86 0.13 100 3.83E−03 −0.46 1.4 hsa-cand110 11.04 10.6810.64 10.96 10.78 0.16 100 6.77E−03 −0.46 1.4 hsa-asg- 11.62 11.80 11.1211.86 11.54 0.25 100 4.83E−02 −0.46 1.4 3811 hsa-asg- 11.11 10.76 11.1910.96 10.95 0.17 100 2.69E−02 −0.46 1.4 6398 hsa-asg- 10.13 9.69 9.9610.03 9.91 0.17 100 1.04E−02 −0.47 1.4 4731 hsa-asg- 10.72 10.41 10.6110.73 10.55 0.18 100 1.28E−02 −0.47 1.4 7096 hsa-asg- 9.63 9.31 9.419.41 9.34 0.18 100 9.17E−03 −0.48 1.4 6367 hsa-asg- 9.86 9.46 9.58 9.949.62 0.22 86 3.31E−02 −0.48 1.4 3456 hsa-asg- 7.00 6.75 6.70 6.84 6.730.21 100 1.84E−02 −0.49 1.4 1641 hsa-asg- 10.38 9.87 10.29 10.21 10.110.21 100 2.78E−02 −0.49 1.4 2980 hsa-asg- 9.10 8.69 8.59 8.97 8.83 0.19100 2.43E−02 −0.49 1.4 4828 hsa-asg- 9.49 9.23 9.02 9.46 9.28 0.18 1008.39E−03 −0.50 1.4 9266 hsa-asg- 11.05 10.69 10.91 10.90 10.82 0.14 1002.61E−03 −0.50 1.4 3260 hsa-asg- 8.46 8.25 8.00 8.43 8.22 0.21 1001.97E−02 −0.50 1.4 6802 hsa-asg- 5.59 6.00 6.20 6.35 5.99 0.27 294.20E−02 −0.51 1.4 7579 hsa-asg- 4.09 4.56 4.64 4.00 4.35 0.28 574.99E−02 −0.51 1.4 12857 hsa-asg- 8.72 8.73 8.52 8.76 8.51 0.24 1002.36E−02 −0.51 1.4 8421 hsa-cand583 6.19 6.06 5.90 5.74 5.94 0.22 292.82E−02 −0.52 1.4 hsa-asg- 9.41 9.05 9.07 9.35 9.18 0.18 100 1.04E−02−0.52 1.4 1123 hsa-asg- 9.57 9.19 9.93 9.42 9.47 0.23 100 3.53E−02 −0.521.4 11946 hsa-asg- 7.69 7.23 7.02 7.15 7.24 0.24 100 3.03E−02 −0.52 1.412953 hsa-asg- 8.98 8.33 8.45 8.74 8.51 0.27 100 4.69E−02 −0.52 1.410639 hsa-asg- 8.33 8.27 8.20 8.53 8.19 0.23 100 2.70E−02 −0.53 1.4 3345hsa-asg- 7.86 7.83 7.38 8.02 7.76 0.20 100 9.44E−03 −0.53 1.4 3230hsa-cand441 7.87 7.60 7.33 7.86 7.58 0.26 29 4.16E−02 −0.53 1.4 hsa-asg-10.74 10.45 10.91 10.81 10.63 0.20 100 2.06E−02 −0.53 1.4 3892 hsa-asg-7.27 6.84 7.04 7.41 7.12 0.18 100 8.27E−03 −0.53 1.4 7326 hsa-asg- 7.417.56 7.22 7.87 7.46 0.23 100 2.38E−02 −0.54 1.5 1174 hsa-asg- 6.85 7.016.85 7.40 7.12 0.29 14 4.14E−02 −0.54 1.5 3290 hsa-asg- 8.55 8.31 7.928.30 8.26 0.20 100 1.76E−02 −0.55 1.5 12441 hsa-asg- 8.20 7.85 7.40 8.127.78 0.32 100 4.82E−02 −0.56 1.5 4502 hsa-asg- 9.63 9.10 9.53 9.42 9.400.17 14 3.42E−03 −0.56 1.5 11758 hsa-asg- 7.26 7.29 6.83 7.51 7.15 0.26100 2.84E−02 −0.57 1.5 4759 hsa-asg- 7.92 7.56 7.86 7.91 7.66 0.24 431.68E−02 −0.57 1.5 3808 hsa-asg- 8.64 8.11 7.94 8.42 8.23 0.26 1002.15E−02 −0.57 1.5 11249 hsa-asg- 10.02 9.77 9.75 9.91 9.75 0.19 1009.17E−03 −0.58 1.5 6728 hsa-asg- 8.03 7.89 7.42 8.11 7.81 0.23 1003.67E−02 −0.58 1.5 2891 hsa-asg- 4.93 5.14 5.55 5.05 5.01 0.28 294.49E−02 −0.58 1.5 14186 hsa-cand735 8.20 7.89 8.31 8.15 8.06 0.16 1001.68E−03 −0.58 1.5 hsa-asg- 5.43 5.59 5.28 5.81 5.58 0.19 14 4.99E−03−0.58 1.5 8391 hsa-asg- 6.94 6.75 6.10 6.71 6.56 0.28 100 2.92E−02 −0.581.5 1617 hsa-asg- 8.86 8.60 8.49 8.91 8.70 0.17 100 2.38E−03 −0.59 1.52626 hsa-asg- 7.19 6.83 6.67 7.47 6.95 0.28 100 3.91E−02 −0.59 1.5 11252hsa-asg- 5.00 5.06 4.81 5.29 5.21 0.32 29 4.64E−02 −0.59 1.5 11585hsa-asg- 7.79 7.31 7.36 7.82 7.61 0.21 100 8.13E−03 −0.59 1.5 10575hsa-asg- 7.73 7.44 7.29 7.69 7.40 0.23 100 1.02E−02 −0.59 1.5 14066hsa-asg- 8.72 8.36 8.81 8.90 8.52 0.28 100 2.43E−02 −0.60 1.5 11729hsa-asg- 8.00 8.25 7.58 7.74 7.80 0.27 100 3.23E−02 −0.60 1.5 11521hsa-asg- 9.05 8.72 8.96 9.05 8.94 0.14 100 8.23E−03 −0.61 1.5 12581hsa-asg- 9.73 9.22 9.47 9.31 9.38 0.18 29 2.49E−03 −0.61 1.5 13079hsa-asg- 5.05 5.54 4.90 5.62 5.30 0.28 14 3.63E−02 −0.61 1.5 7569hsa-asg- 9.22 8.86 8.71 8.98 8.94 0.19 100 9.37E−03 −0.62 1.5 3462hsa-asg- 7.07 6.84 6.93 7.25 7.00 0.27 100 4.53E−02 −0.62 1.5 8227hsa-asg-508 6.71 6.64 6.35 6.92 6.48 0.31 86 3.32E−02 −0.62 1.5 hsa-asg-7.97 7.72 7.47 8.34 7.78 0.29 100 4.94E−02 −0.62 1.5 2580 hsa-asg- 7.647.68 7.56 7.85 7.60 0.28 100 2.31E−02 −0.63 1.5 7687 hsa-asg-688 7.917.40 8.14 7.54 7.69 0.25 100 1.49E−02 −0.63 1.5 hsa-asg- 8.25 8.21 7.688.28 8.02 0.28 100 1.79E−02 −0.63 1.6 3746 hsa-asg- 5.68 5.33 5.66 5.955.54 0.30 14 4.42E−02 −0.64 1.6 2041 hsa-asg-819 8.17 7.75 7.65 8.077.76 0.32 100 4.78E−02 −0.64 1.6 hsa-asg- 5.08 5.45 4.66 5.07 5.02 0.270 1.64E−02 −0.64 1.6 4200 hsa-asg- 6.76 7.17 7.08 7.45 7.19 0.26 1002.23E−02 −0.64 1.6 4469 hsa-asg- 5.29 5.04 5.02 5.67 5.16 0.30 292.09E−02 −0.65 1.6 10183 hsa-cand523 6.46 6.15 6.12 6.18 6.11 0.22 435.92E−03 −0.65 1.6 hsa-asg- 6.44 6.76 6.28 6.57 6.42 0.24 43 3.54E−02−0.65 1.6 8393 hsa-asg- 6.37 6.69 6.70 6.41 6.66 0.21 100 4.15E−03 −0.661.6 9729 hsa-asg- 6.70 6.46 6.33 6.83 6.59 0.18 43 6.64E−03 −0.66 1.66675 hsa-asg- 8.37 8.11 8.23 8.18 8.11 0.22 100 4.42E−03 −0.66 1.6 1813hsa-asg- 6.57 6.24 5.98 6.75 6.38 0.24 71 8.03E−03 −0.67 1.6 8562hsa-asg- 7.46 7.71 7.43 7.38 7.45 0.35 100 3.60E−02 −0.67 1.6 11479hsa-asg- 9.64 9.49 8.79 9.50 9.22 0.32 14 2.54E−02 −0.67 1.6 5379hsa-asg- 6.34 6.16 6.25 6.45 6.24 0.30 14 1.80E−02 −0.68 1.6 11563hsa-asg- 7.55 7.05 6.97 7.26 7.20 0.18 100 2.32E−03 −0.68 1.6 2131hsa-asg- 6.87 6.46 6.44 6.87 6.71 0.22 100 1.89E−02 −0.69 1.6 5289hsa-asg- 6.47 6.45 6.25 6.98 6.63 0.31 43 2.33E−02 −0.69 1.6 8565hsa-asg- 6.09 5.92 5.78 5.65 5.76 0.20 57 2.21E−03 −0.69 1.6 4599hsa-asg- 6.26 5.77 5.83 6.61 6.12 0.39 29 4.43E−02 −0.70 1.6 3340hsa-asg- 6.56 6.17 6.75 6.27 6.39 0.20 71 7.84E−03 −0.70 1.6 3694hsa-asg- 12.36 12.02 12.29 12.59 12.37 0.22 100 9.45E−03 −0.70 1.6 9530hsa-asg- 8.21 8.11 7.37 8.26 7.95 0.34 100 3.43E−02 −0.70 1.6 5085hsa-asg- 6.83 6.56 7.22 6.82 6.85 0.24 71 7.23E−03 −0.71 1.6 2588hsa-asg- 6.93 7.18 6.82 7.21 7.03 0.18 100 2.20E−03 −0.71 1.6 5061hsa-asg- 8.41 8.05 7.85 8.36 8.13 0.24 100 4.88E−03 −0.72 1.6 11155hsa-asg- 3.97 4.10 4.24 3.72 4.01 0.17 14 2.90E−03 −0.72 1.6 8672hsa-asg- 7.38 7.25 6.46 7.11 7.00 0.33 100 3.40E−02 −0.72 1.7 1576hsa-asg- 6.62 6.35 6.43 6.59 6.31 0.37 29 4.16E−02 −0.72 1.7 7730hsa-asg- 9.92 9.61 9.48 9.80 9.78 0.19 86 4.74E−03 −0.73 1.7 12247hsa-asg- 7.72 7.52 7.04 7.49 7.25 0.33 100 2.01E−02 −0.73 1.7 9511hsa-asg- 6.41 6.43 5.55 5.73 6.02 0.33 71 2.24E−02 −0.73 1.7 8959hsa-asg-797 5.43 5.38 5.14 5.48 5.32 0.14 14 5.40E−04 −0.73 1.7hsa-asg-271 4.53 4.56 4.21 4.91 4.46 0.25 14 5.85E−03 −0.73 1.7 hsa-asg-11.61 11.16 11.31 11.87 11.49 0.27 100 3.14E−02 −0.73 1.7 2750 hsa-asg-5.84 6.63 6.66 6.01 6.31 0.34 86 3.14E−02 −0.74 1.7 2693 hsa-asg-5310.14 9.70 9.68 10.04 9.85 0.17 100 9.81E−04 −0.74 1.7 hsa-asg- 9.529.33 9.65 9.45 9.37 0.19 100 1.20E−02 −0.74 1.7 5032 hsa-asg- 7.51 7.237.06 7.41 7.25 0.16 100 3.18E−03 −0.74 1.7 6892 hsa-asg- 7.79 6.85 7.006.75 6.99 0.38 100 3.64E−02 −0.75 1.7 1911 hsa-asg- 7.13 6.79 6.72 7.046.73 0.33 86 4.95E−02 −0.75 1.7 8675 hsa-asg-809 6.51 6.49 5.76 6.386.18 0.35 57 2.63E−02 −0.75 1.7 hsa-asg- 8.03 7.57 7.81 8.30 7.87 0.25100 2.50E−02 −0.75 1.7 11893 hsa-asg- 8.18 8.03 7.48 8.27 7.93 0.28 1004.33E−02 −0.75 1.7 1861 hsa-asg- 6.00 6.13 6.08 6.14 6.19 0.24 1003.52E−03 −0.77 1.7 6651 hsa-asg-471 8.22 8.20 8.26 8.17 8.18 0.07 1002.36E−05 −0.77 1.7 hsa-asg- 5.59 6.32 5.55 5.65 5.92 0.40 14 3.65E−02−0.77 1.7 10861 hsa-asg- 8.44 8.83 8.12 8.89 8.53 0.28 100 8.08E−03−0.78 1.7 4310 hsa-asg- 7.97 8.26 7.75 8.05 8.06 0.41 100 3.48E−02 −0.801.7 7153 hsa-asg- 5.48 5.71 5.49 6.18 5.74 0.26 43 8.95E−03 −0.81 1.86879 hsa-asg- 5.90 5.88 5.66 6.63 6.04 0.33 14 1.45E−02 −0.81 1.8 7352hsa-asg- 7.54 7.35 7.24 7.28 7.45 0.25 100 3.40E−03 −0.82 1.8 12775hsa-asg- 8.06 8.07 7.04 8.28 7.91 0.40 100 3.40E−02 −0.83 1.8 13613hsa-asg- 2.27 2.59 2.75 3.39 2.75 0.40 0 3.92E−02 −0.83 1.8 9748hsa-asg- 6.88 7.06 6.84 7.05 6.89 0.13 100 1.29E−03 −0.83 1.8 7640hsa-asg- 7.41 6.54 6.90 7.16 6.90 0.31 100 1.01E−02 −0.84 1.8 10971hsa-asg- 9.64 8.99 9.85 9.19 9.33 0.33 14 1.95E−02 −0.84 1.8 6092hsa-asg-813 5.40 5.18 4.90 6.22 5.47 0.43 14 3.47E−02 −0.84 1.8 hsa-asg-7.79 7.54 7.17 7.98 7.60 0.27 100 3.99E−03 −0.84 1.8 6437 hsa-asg- 8.508.10 8.12 8.15 8.27 0.17 100 2.71E−04 −0.84 1.8 12946 hsa-asg- 8.90 8.288.40 8.89 8.53 0.26 100 3.02E−03 −0.85 1.8 6948 hsa-asg- 7.97 7.23 7.518.28 7.68 0.39 43 2.85E−02 −0.86 1.8 11486 hsa-cand711 10.23 9.89 9.9010.09 9.98 0.14 100 6.98E−05 −0.86 1.8 hsa-cand588 6.47 6.23 6.52 7.436.66 0.45 86 3.57E−02 −0.88 1.8 hsa-asg-555 7.31 7.21 7.52 7.62 7.210.34 100 2.71E−02 −0.88 1.8 hsa-asg- 7.73 7.47 8.56 8.09 7.80 0.41 432.37E−02 −0.88 1.8 6149 hsa-asg- 8.09 7.91 7.21 8.35 7.76 0.40 433.83E−02 −0.88 1.8 9920 hsa-asg- 7.44 6.84 6.84 7.56 7.16 0.28 1001.60E−02 −0.88 1.8 1391 hsa-asg- 7.39 7.38 6.75 7.57 7.06 0.41 1002.63E−02 −0.88 1.8 3150 hsa-asg- 7.02 7.19 6.25 7.30 7.00 0.42 863.49E−02 −0.88 1.8 13829 hsa-asg- 7.26 7.18 6.44 7.26 6.79 0.48 864.66E−02 −0.88 1.8 9382 hsa-cand855 7.29 6.56 6.86 6.91 6.79 0.34 711.42E−02 −0.88 1.8 hsa-asg- 8.40 7.98 7.22 8.52 7.99 0.42 100 2.42E−02−0.89 1.9 10716 hsa-asg- 9.09 8.81 8.38 8.97 8.80 0.27 100 3.11E−03−0.90 1.9 11698 hsa-cand902 6.04 6.44 5.81 6.41 6.09 0.33 29 8.47E−03−0.90 1.9 hsa-asg- 9.36 8.84 8.89 9.14 9.16 0.24 100 1.60E−03 −0.92 1.912919 hsa-asg- 5.97 4.73 5.44 5.66 5.34 0.42 14 2.03E−02 −0.92 1.9 4261hsa-asg- 7.20 7.05 7.54 7.10 7.22 0.16 100 1.72E−04 −0.92 1.9 1153hsa-asg- 6.37 6.30 6.16 6.91 6.43 0.28 71 1.75E−02 −0.92 1.9 3841hsa-asg- 11.47 11.22 11.49 11.61 11.44 0.27 100 6.61E−03 −0.92 1.9 3231hsa-asg-225 5.62 5.40 5.81 6.06 5.48 0.36 14 1.67E−02 −0.93 1.9 hsa-asg-8.14 8.20 7.51 8.38 7.92 0.34 100 1.26E−02 −0.93 1.9 2017 hsa-asg- 7.597.31 7.03 8.04 7.33 0.37 100 1.23E−02 −0.93 1.9 6623 hsa-asg- 6.20 5.865.96 5.75 5.83 0.21 29 6.69E−04 −0.93 1.9 9526 hsa-asg- 6.45 5.24 5.815.74 5.88 0.48 14 3.57E−02 −0.93 1.9 3014 hsa-asg- 7.36 8.29 7.94 8.007.93 0.48 100 3.31E−02 −0.94 1.9 12957 hsa-asg- 4.62 5.01 4.00 4.81 4.760.38 14 1.68E−02 −0.95 1.9 2564 hsa-asg- 5.84 5.33 5.78 5.92 5.75 0.2029 5.26E−03 −0.96 2.0 10473 hsa-asg- 4.09 4.14 3.68 4.20 3.75 0.43 02.32E−02 −0.97 2.0 8303 hsa-asg- 5.58 6.30 6.32 6.18 6.26 0.40 1001.94E−02 −0.97 2.0 11310 hsa-asg- 7.03 6.70 6.68 6.74 6.83 0.33 1005.73E−03 −0.97 2.0 6831 hsa-asg- 7.41 8.23 7.88 7.80 7.91 0.49 713.60E−02 −0.97 2.0 11373 hsa-asg- 6.69 7.48 7.00 7.31 7.10 0.33 1004.64E−03 −1.00 2.0 6005 hsa-asg- 10.16 9.78 9.73 9.76 9.73 0.22 861.76E−03 −1.01 2.0 4654 hsa-asg- 8.59 8.50 8.40 8.84 8.55 0.42 1001.56E−02 −1.01 2.0 9403 hsa-asg- 9.08 8.61 8.72 8.94 8.71 0.30 1002.51E−03 −1.01 2.0 7893 hsa-asg- 7.76 8.15 9.01 8.03 8.19 0.51 1003.54E−02 −1.05 2.1 13000 hsa-asg- 6.34 7.03 7.09 7.35 6.98 0.47 1002.02E−02 −1.05 2.1 10883 hsa-asg- 6.19 6.11 6.37 6.57 6.12 0.27 293.24E−03 −1.06 2.1 1689 hsa-asg- 7.21 7.45 6.33 7.66 7.14 0.52 714.62E−02 −1.06 2.1 9412 hsa-asg- 9.06 8.59 9.13 8.71 8.73 0.26 1002.57E−03 −1.07 2.1 3271 hsa-asg- 7.92 7.41 6.44 7.75 7.41 0.49 863.10E−02 −1.08 2.1 13559 hsa-asg- 4.77 4.75 5.31 5.52 4.81 0.45 141.38E−02 −1.09 2.1 11898 hsa-asg- 4.15 4.90 4.39 5.46 4.53 0.49 01.97E−02 −1.10 2.1 3081 hsa-asg- 7.29 6.62 6.20 7.41 6.82 0.42 712.08E−02 −1.10 2.1 2858 hsa-asg-278 6.33 6.52 5.76 6.70 6.08 0.55 433.82E−02 −1.11 2.2 hsa-asg- 9.23 9.06 8.97 9.07 8.91 0.23 100 4.65E−04−1.11 2.2 5423 hsa-asg- 5.08 5.77 5.66 5.90 5.58 0.31 29 2.50E−03 −1.112.2 9257 hsa-asg- 7.31 6.58 6.82 7.81 7.05 0.48 100 1.85E−02 −1.12 2.210197 hsa-asg- 8.49 7.73 7.72 8.15 8.13 0.32 100 2.69E−03 −1.14 2.2 6507hsa-asg- 6.64 6.24 6.27 7.16 6.62 0.33 86 5.68E−03 −1.14 2.2 9015hsa-asg- 3.00 3.03 3.30 2.47 3.00 0.32 0 2.23E−03 −1.15 2.2 5460hsa-asg- 10.06 10.04 9.91 10.57 10.19 0.44 100 9.65E−03 −1.16 2.2 13308hsa-asg- 6.30 5.56 5.28 6.05 5.79 0.34 29 5.93E−03 −1.16 2.2 4440hsa-asg- 7.26 7.20 6.71 7.54 7.13 0.26 100 3.42E−03 −1.16 2.2 1375hsa-asg- 7.11 6.74 6.28 6.97 6.74 0.31 71 1.16E−02 −1.17 2.3 8763hsa-asg- 8.90 10.21 9.61 9.90 9.76 0.54 100 2.17E−02 −1.19 2.3 2872hsa-asg- 7.41 8.16 7.48 7.69 7.83 0.42 100 6.30E−03 −1.20 2.3 12460hsa-asg- 6.64 6.59 6.28 6.91 6.44 0.40 29 5.62E−03 −1.21 2.3 8138hsa-asg- 10.21 10.17 10.09 10.64 10.31 0.38 100 3.56E−03 −1.21 2.3 5304hsa-asg- 6.63 6.25 6.49 6.79 6.41 0.26 14 1.14E−02 −1.21 2.3 4971hsa-asg- 7.85 7.27 7.45 7.45 7.38 0.25 100 6.72E−04 −1.23 2.4 9343hsa-asg- 6.19 6.52 6.21 6.96 6.36 0.31 71 1.00E−03 −1.24 2.4 8271hsa-asg- 7.70 8.18 8.07 8.66 8.30 0.35 100 4.90E−03 −1.25 2.4 13260hsa-asg- 6.17 6.19 5.63 4.94 5.54 0.62 14 2.98E−02 −1.26 2.4 12105hsa-asg- 4.21 4.68 3.03 4.10 4.06 0.66 29 4.45E−02 −1.26 2.4 7685hsa-asg- 6.53 6.61 7.48 7.28 6.94 0.42 100 4.87E−03 −1.27 2.4 3360hsa-asg- 5.45 5.95 6.49 6.27 6.06 0.49 57 2.32E−02 −1.28 2.4 5576hsa-asg- 6.34 5.88 5.40 6.34 5.94 0.40 29 1.37E−02 −1.29 2.4 4843hsa-asg- 5.77 6.28 5.90 6.18 6.17 0.49 29 1.41E−02 −1.29 2.4 7009hsa-asg- 4.98 4.83 5.34 4.76 5.04 0.29 0 7.84E−03 −1.30 2.5 8928hsa-asg- 5.90 5.78 5.55 6.29 5.67 0.38 14 4.72E−03 −1.32 2.5 12777hsa-asg- 5.29 6.38 6.35 6.54 6.27 0.59 43 2.15E−02 −1.35 2.6 7069hsa-cand965 5.89 7.57 7.23 7.51 7.29 0.66 29 4.98E−02 −1.38 2.6 hsa-asg-5.92 5.95 6.08 6.24 6.06 0.18 43 1.88E−03 −1.38 2.6 13693 hsa-asg- 6.406.00 5.14 6.55 6.05 0.45 43 7.16E−03 −1.40 2.6 8329 hsa-asg- 7.21 6.856.38 7.46 6.74 0.52 71 1.05E−02 −1.43 2.7 3597 hsa-asg- 5.99 6.19 6.055.82 6.05 0.30 57 6.21E−03 −1.44 2.7 9852 hsa-asg- 4.29 5.06 4.45 4.184.41 0.34 0 1.68E−03 −1.45 2.7 3107 hsa-asg- 12.09 11.62 12.09 12.3412.07 0.52 100 7.47E−03 −1.47 2.8 14245 hsa-asg- 5.55 6.37 5.92 6.276.05 0.30 29 1.95E−03 −1.47 2.8 6949 hsa-asg- 5.51 5.67 5.34 5.86 5.540.20 57 2.22E−04 −1.52 2.9 7319 hsa-asg- 6.32 6.22 5.63 6.72 6.30 0.4543 7.44E−03 −1.52 2.9 9022 hsa-asg- 6.52 6.80 5.94 6.66 6.40 0.34 715.08E−04 −1.56 2.9 12246 hsa-asg- 7.13 7.51 7.37 7.66 7.46 0.56 866.76E−03 −1.58 3.0 8917 hsa-asg- 6.60 6.87 6.20 6.42 6.85 0.51 1005.18E−03 −1.60 3.0 13244 hsa-cand345 6.17 6.16 5.85 6.83 6.10 0.41 711.58E−03 −1.61 3.0 hsa-asg- 5.99 7.07 7.51 7.63 7.32 0.78 100 4.16E−02−1.62 3.1 12702 hsa-asg- 6.78 6.78 6.67 6.76 6.84 0.17 100 2.98E−04−1.67 3.2 13230 hsa-asg- 6.74 6.97 5.90 6.60 6.84 0.58 100 1.10E−02−1.69 3.2 12902 hsa-asg- 6.50 7.57 6.95 7.53 7.17 0.39 100 2.48E−03−1.71 3.3 13237 hsa-asg- 6.01 7.72 7.23 7.51 7.13 0.85 86 2.80E−02 −1.753.4 4002 hsa-asg-21 4.64 6.59 6.30 6.40 6.05 0.75 86 3.18E−02 −1.77 3.4hsa-asg- 6.80 7.78 7.50 7.81 7.62 0.48 86 2.61E−03 −1.79 3.5 10149hsa-asg- 7.20 7.17 6.77 6.40 6.97 0.53 100 2.85E−02 −1.83 3.6 5303hsa-asg- 4.64 6.80 5.76 6.43 6.24 0.83 71 4.84E−02 −1.84 3.6 10666hsa-asg-335 6.22 8.78 7.94 8.01 7.82 1.01 100 4.11E−02 −1.88 3.7hsa-asg- 5.40 7.00 6.32 6.33 6.21 0.61 29 3.93E−03 −1.98 3.9 6250hsa-asg- 4.84 6.07 5.90 7.15 6.08 0.78 29 1.18E−02 −1.98 4.0 8208hsa-asg- 6.14 8.68 8.01 8.23 7.78 1.09 100 4.01E−02 −2.04 4.1 10387hsa-asg- 9.18 8.89 8.86 10.09 9.20 0.58 100 1.86E−03 −2.09 4.2 13248hsa-asg- 4.58 6.46 6.44 5.31 5.67 0.87 71 2.27E−02 −2.12 4.4 12079hsa-asg- 7.60 8.20 7.32 7.98 7.57 0.40 100 5.68E−04 −2.13 4.4 4557hsa-asg- 8.05 8.75 8.88 9.64 8.92 0.62 100 1.81E−02 −2.17 4.5 13937hsa-miR165 6.70 5.83 5.14 5.80 5.55 0.63 14 7.52E−03 −2.18 4.5 hsa-asg-5.81 6.86 6.68 7.27 6.65 0.96 86 1.84E−02 −2.24 4.7 8682 hsa-asg- 4.736.11 5.83 5.74 5.71 0.61 29 4.97E−03 −2.27 4.8 6413 hsa-asg- 6.29 6.826.28 7.15 6.60 0.42 86 1.45E−03 −2.29 4.9 13304 hsa-asg- 4.46 6.75 6.286.63 5.93 1.04 71 3.02E−02 −2.29 4.9 4205 hsa-asg-870 5.18 6.42 6.056.50 6.12 1.05 43 1.74E−02 −2.44 5.4 hsa-asg- 9.80 10.34 10.32 10.7610.27 0.34 100 1.37E−03 −2.46 5.5 7074 hsa-asg- 7.36 7.25 6.90 7.66 7.420.37 100 9.08E−03 −2.48 5.6 13279 hsa-asg- 2.95 3.48 3.59 3.49 3.56 0.770 2.06E−05 6.71 104.3 5638 hsa-asg- 3.70 4.47 3.68 4.15 3.96 0.36 01.13E−04 4.34 20.3 11338 hsa-asg- 3.70 2.91 4.51 3.49 4.04 1.22 146.21E−03 4.10 17.2 5630 hsa-asg- 2.81 2.78 2.63 2.47 2.82 0.34 01.66E−03 2.91 7.5 13997 hsa-asg- 3.27 3.68 2.28 2.72 2.97 0.44 01.45E−03 2.77 6.8 8428 hsa-asg- 5.21 5.12 5.06 5.21 5.18 0.53 0 3.29E−042.64 6.2 9186 hsa-asg- 3.23 2.91 3.19 2.97 3.01 0.22 0 5.93E−04 2.57 5.91296 hsa-asg- 2.52 3.15 2.98 2.81 2.87 0.34 0 5.24E−05 2.52 5.7 9723hsa-asg- 3.09 3.37 3.30 2.81 3.09 0.34 0 1.05E−03 2.23 4.7 11380hsa-asg- 3.14 3.09 3.19 4.00 3.29 0.33 0 2.75E−03 2.10 4.3 2261 hsa-asg-6.03 7.32 6.38 5.77 6.48 0.67 100 6.21E−03 2.10 4.3 1519 hsa-asg- 3.183.37 2.40 2.30 3.08 0.62 0 7.11E−03 2.09 4.3 1794 hsa-asg- 4.32 4.174.90 4.97 4.55 0.29 0 9.72E−05 2.03 4.1 12271 hsa-asg- 4.21 3.98 5.145.46 4.38 0.67 0 1.68E−02 2.03 4.1 14208 hsa-asg- 4.29 4.25 3.49 3.863.78 0.40 0 1.35E−02 1.93 3.8 8486 hsa-asg- 8.38 8.23 8.84 9.13 8.700.44 14 7.16E−04 1.90 3.7 5640 hsa-cand317 3.88 3.48 4.61 4.91 3.90 0.7414 4.34E−02 1.71 3.3 hsa-asg- 3.52 3.15 2.63 3.56 3.32 0.41 0 2.08E−031.71 3.3 2335 hsa-asg- 2.22 3.03 3.09 2.81 2.96 0.37 0 1.77E−02 1.70 3.31587 hsa-asg- 4.09 5.41 2.86 5.11 4.34 0.86 0 4.25E−02 1.63 3.1 4803hsa-asg- 9.37 8.64 9.11 10.21 9.28 0.60 43 9.15E−03 1.59 3.0 13278hsa-asg-497 5.64 6.69 5.31 5.62 5.78 0.43 57 2.03E−03 1.55 2.9 hsa-asg-4.15 4.47 3.09 5.08 4.16 0.81 0 4.31E−02 1.52 2.9 9796 hsa-asg- 3.484.14 3.49 4.89 3.97 0.60 0 3.98E−02 1.50 2.8 10031 hsa-asg- 4.73 3.904.07 4.83 4.28 0.41 0 2.10E−03 1.50 2.8 9980 hsa-asg- 4.51 4.53 4.074.79 4.42 0.23 0 1.29E−02 1.49 2.8 12453 hsa-asg- 5.52 5.81 5.28 4.815.25 0.38 0 2.53E−02 1.46 2.7 11489 hsa-asg- 3.81 3.48 3.49 3.28 3.360.28 0 2.61E−04 1.45 2.7 11361 hsa-asg- 6.29 5.45 6.12 5.04 5.49 0.52 298.72E−03 1.43 2.7 12807 hsa-asg- 6.14 4.31 5.14 4.71 4.92 0.59 141.76E−02 1.38 2.6 7511 hsa-asg- 4.82 4.44 4.33 4.67 4.72 0.26 0 1.41E−021.35 2.5 9762 hsa-asg- 6.82 6.39 5.78 6.93 6.40 0.52 0 1.63E−02 1.29 2.412372 hsa-asg- 7.73 7.15 7.34 8.45 7.66 0.64 43 3.47E−02 1.29 2.4 4223hsa-asg- 5.93 5.01 4.71 5.08 5.41 0.48 43 1.60E−02 1.24 2.4 11402hsa-asg- 6.60 6.15 5.96 6.66 6.38 0.25 71 4.50E−04 1.24 2.4 8633hsa-asg- 5.05 6.19 5.61 5.74 5.71 0.44 0 1.11E−02 1.21 2.3 4780 hsa-asg-6.41 5.66 6.18 5.47 5.83 0.35 0 3.21E−03 1.15 2.2 9117 hsa-asg- 4.625.36 4.71 4.44 4.60 0.44 14 1.97E−02 1.14 2.2 1362 hsa-asg- 9.36 9.859.37 9.86 9.44 0.42 100 9.27E−03 1.13 2.2 14233 hsa-asg- 12.13 11.2611.31 11.44 11.70 0.45 100 1.89E−02 1.10 2.1 13309 hsa-asg- 7.28 6.385.94 6.86 6.65 0.42 71 3.49E−02 1.10 2.1 13841 hsa-asg- 6.27 7.18 6.385.73 6.23 0.48 43 1.83E−02 1.10 2.1 14001 hsa-asg- 3.97 4.47 5.17 4.004.59 0.49 0 2.24E−02 1.06 2.1 12320 hsa-asg- 6.47 6.61 6.00 7.06 6.550.33 100 1.63E−02 1.05 2.1 9227 hsa-asg- 5.79 6.11 6.05 6.36 6.12 0.2143 4.61E−04 1.04 2.1 7922 hsa-asg- 8.69 8.11 8.21 8.21 8.25 0.29 1002.10E−03 1.02 2.0 9261 hsa-cand732 7.38 6.50 6.81 7.13 6.80 0.39 571.66E−02 1.02 2.0 hsa-asg- 5.18 5.18 4.94 4.81 5.03 0.21 0 3.23E−04 1.022.0 13924 hsa-asg- 4.64 4.10 4.14 5.59 4.63 0.55 0 4.52E−02 1.02 2.02889 hsa-asg- 6.53 6.15 5.83 6.42 6.10 0.30 29 5.93E−03 1.02 2.0 12381hsa-asg- 6.98 6.39 7.03 5.92 6.54 0.39 86 1.15E−02 1.02 2.0 8003hsa-asg- 5.08 5.08 5.31 5.34 5.06 0.26 0 6.75E−03 1.01 2.0 7262 hsa-asg-10.26 10.37 9.46 10.36 10.02 0.35 100 4.04E−02 0.99 2.0 11850 hsa-asg-6.01 5.27 5.81 5.74 5.76 0.24 0 1.22E−03 0.98 2.0 7027 hsa-asg- 7.967.77 7.71 8.37 7.87 0.30 71 6.10E−03 0.97 2.0 12937 hsa-asg-345 4.584.44 4.76 4.56 4.71 0.19 0 3.29E−04 0.97 2.0 hsa-asg- 5.46 5.40 4.985.11 5.42 0.44 43 2.66E−02 0.96 1.9 4969 hsa-asg- 5.65 5.73 4.98 5.265.44 0.30 0 1.79E−02 0.96 1.9 3798 hsa-asg- 6.56 6.00 6.94 5.95 6.200.40 43 1.88E−02 0.95 1.9 4703 hsa-asg- 3.14 3.32 3.30 3.46 3.12 0.45 04.96E−02 0.95 1.9 8792 hsa-asg- 5.35 4.97 5.06 4.60 5.05 0.38 0 1.27E−020.94 1.9 4460 hsa-asg- 7.24 7.11 6.96 7.43 7.12 0.29 0 5.18E−03 0.94 1.94026 hsa-cand421 10.62 10.83 10.39 10.88 10.62 0.26 100 1.61E−02 0.931.9 hsa-asg- 7.79 7.34 7.21 7.93 7.59 0.41 100 1.88E−02 0.93 1.9 3670hsa-asg- 7.49 7.14 7.29 7.16 7.22 0.16 100 1.22E−03 0.88 1.8 9130hsa-asg- 5.40 5.54 5.28 4.95 5.43 0.28 29 4.00E−02 0.88 1.8 7783hsa-asg- 5.22 5.81 5.02 4.94 5.27 0.39 43 3.35E−02 0.86 1.8 3944hsa-asg- 5.00 5.40 4.94 4.60 4.93 0.30 0 6.39E−03 0.86 1.8 13798hsa-asg- 8.33 8.04 8.15 8.43 8.11 0.31 100 8.28E−03 0.86 1.8 6870hsa-asg- 4.93 5.14 5.37 4.67 4.85 0.37 14 2.70E−02 0.85 1.8 2460hsa-asg- 4.18 4.17 4.21 4.81 4.43 0.28 14 3.48E−02 0.84 1.8 11267hsa-asg- 5.49 6.08 5.31 5.38 5.46 0.29 43 8.12E−03 0.83 1.8 3604hsa-asg- 5.86 6.47 6.47 5.82 6.05 0.30 43 9.81E−03 0.80 1.7 4296hsa-asg- 8.80 8.21 7.77 8.07 8.19 0.35 100 4.51E−02 0.76 1.7 8866hsa-asg- 4.97 5.14 4.56 4.81 4.88 0.21 100 5.92E−03 0.73 1.7 12471hsa-asg- 7.39 7.30 7.70 7.93 7.49 0.31 100 3.18E−02 0.72 1.7 11515hsa-asg- 5.59 6.03 5.92 5.52 5.80 0.26 71 1.70E−02 0.71 1.6 7252hsa-asg- 6.02 5.16 5.83 5.67 5.82 0.38 57 4.23E−02 0.70 1.6 11458hsa-asg- 9.03 8.98 8.45 8.74 8.71 0.28 0 1.98E−02 0.69 1.6 13453hsa-asg- 9.58 9.30 9.81 9.23 9.35 0.25 100 9.03E−03 0.68 1.6 3722hsa-asg- 10.93 10.94 10.45 11.11 10.84 0.20 100 3.58E−02 0.67 1.6 3992hsa-asg- 6.49 6.57 5.96 6.12 6.34 0.23 14 1.39E−02 0.66 1.6 4373hsa-asg- 7.22 7.00 6.92 7.50 7.08 0.21 100 4.17E−03 0.66 1.6 9480hsa-asg- 12.68 12.56 12.44 12.69 12.59 0.12 100 9.16E−03 0.65 1.6 7939hsa-asg- 5.69 6.54 6.30 6.25 6.22 0.28 100 1.86E−02 0.64 1.6 4448hsa-asg- 6.42 5.81 5.94 6.19 6.20 0.26 71 2.18E−02 0.64 1.6 1338hsa-asg- 8.09 7.62 7.25 7.70 7.53 0.30 100 2.54E−02 0.63 1.5 11617hsa-asg-954 9.66 9.68 9.05 9.09 9.31 0.26 100 1.62E−02 0.62 1.5 hsa-asg-5.85 6.15 5.63 6.58 6.01 0.33 86 4.50E−02 0.62 1.5 2381 hsa-asg- 4.985.45 5.34 5.39 5.35 0.30 14 2.72E−02 0.62 1.5 2697 hsa-asg- 5.35 5.755.83 5.70 5.66 0.18 0 3.79E−03 0.59 1.5 2365 hsa-asg- 5.21 5.20 5.214.89 5.09 0.12 0 7.55E−03 0.59 1.5 9795 hsa-asg- 5.25 5.22 4.90 5.214.94 0.30 14 4.64E−02 0.59 1.5 12457 hsa-asg- 6.02 6.21 5.47 5.40 5.770.31 14 3.91E−02 0.58 1.5 2500 hsa-asg- 6.84 6.79 6.76 6.83 6.84 0.12100 3.51E−02 0.56 1.5 8882 hsa-asg- 5.65 5.64 5.34 5.42 5.37 0.22 571.38E−02 0.55 1.5 5973 hsa-asg- 5.82 6.23 5.96 5.99 6.07 0.26 863.62E−02 0.54 1.5 6616 hsa-asg- 6.47 6.12 6.14 6.22 6.29 0.14 862.95E−03 0.53 1.4 13294 hsa-asg- 5.16 4.92 5.14 5.19 4.96 0.29 574.22E−02 0.53 1.4 9521 hsa-asg- 8.92 8.46 8.57 8.69 8.56 0.21 1001.63E−02 0.52 1.4 7450 hsa-asg- 4.39 5.01 4.61 4.40 4.53 0.23 142.83E−02 0.51 1.4 4030 hsa-asg- 7.43 6.96 6.86 6.90 6.98 0.25 1004.49E−02 0.50 1.4 12406 hsa-asg- 5.65 5.46 5.52 5.53 5.64 0.15 574.11E−03 0.48 1.4 12885 hsa-cand873 5.54 5.88 5.47 5.33 5.42 0.26 434.38E−02 0.48 1.4 hsa-asg- 13.31 13.25 13.23 13.29 13.30 0.06 1006.79E−03 0.47 1.4 14201 hsa-asg- 10.47 10.19 10.43 10.26 10.32 0.18 1004.15E−02 0.46 1.4 8568 hsa-asg- 8.01 7.49 7.88 7.61 7.68 0.19 573.96E−02 0.45 1.4 3239 hsa-asg- 7.39 7.25 7.18 7.03 7.17 0.13 1002.58E−03 0.45 1.4 4984 hsa-asg- 5.77 5.99 5.71 5.62 5.79 0.15 577.62E−03 0.44 1.4 2093 5.95 6.06 5.92 5.70 5.96 0.18 100 1.61E−02 0.431.3 hsa-asg-733 hsa-asg-89 6.20 6.37 6.20 5.86 6.15 0.17 100 1.60E−020.40 1.3 hsa-asg- 5.80 5.83 5.74 5.49 5.73 0.15 100 1.12E−02 0.39 1.312816 hsa-asg- 5.21 5.49 5.44 5.44 5.45 0.18 0 2.11E−02 0.39 1.3 11057hsa-asg- 6.34 6.35 6.08 6.07 6.20 0.12 100 9.20E−03 0.36 1.3 6305hsa-asg- 5.52 5.64 5.58 5.33 5.59 0.19 14 3.93E−02 0.36 1.3 4587hsa-asg- 6.02 5.83 5.96 5.84 5.92 0.08 100 4.34E−02 0.16 1.1 2483hsa-asg-758 6.66 6.55 6.28 6.54 6.51 0.16 100 4.22E−02 −0.29 1.2hsa-asg- 6.55 6.54 6.75 6.65 6.61 0.14 100 2.89E−02 −0.30 1.2 7410hsa-asg- 10.56 10.07 10.35 10.32 10.30 0.16 100 3.48E−02 −0.33 1.3 2899hsa-asg- 9.43 9.22 9.31 9.60 9.33 0.17 29 4.56E−02 −0.35 1.3 7628hsa-asg- 10.85 10.63 10.84 10.82 10.77 0.15 100 1.74E−02 −0.35 1.3 14034hsa-asg- 10.07 9.70 10.10 10.17 9.91 0.20 100 4.83E−02 −0.37 1.3 8253hsa-asg- 4.97 5.14 4.90 4.97 4.95 0.17 14 2.20E−02 −0.38 1.3 4426hsa-asg- 7.04 6.74 6.66 6.77 6.75 0.16 100 3.73E−02 −0.38 1.3 8655hsa-asg- 11.69 11.27 11.38 11.58 11.45 0.15 100 1.44E−02 −0.39 1.3 10632hsa-asg-974 6.87 6.87 6.86 7.24 6.99 0.22 100 4.65E−02 −0.39 1.3hsa-asg- 6.59 6.29 6.27 6.81 6.48 0.20 29 3.65E−02 −0.39 1.3 7218hsa-asg-206 9.93 9.51 9.64 9.98 9.76 0.16 100 3.84E−02 −0.40 1.3hsa-asg- 8.12 7.98 7.84 7.71 7.88 0.21 100 3.39E−02 −0.41 1.3 3565hsa-cand559 7.23 7.44 7.52 7.40 7.39 0.19 100 2.71E−02 −0.41 1.3hsa-asg- 8.43 8.28 8.16 8.65 8.40 0.23 100 4.36E−02 −0.42 1.3 1809hsa-asg- 9.02 8.49 8.85 8.86 8.74 0.18 100 1.81E−02 −0.42 1.3 4020hsa-asg- 8.42 8.16 8.21 8.48 8.27 0.17 100 1.56E−02 −0.42 1.3 10392hsa-asg- 6.63 6.35 6.50 6.82 6.53 0.17 43 1.43E−02 −0.42 1.3 9501hsa-asg-976 6.74 6.94 6.41 6.63 6.65 0.23 100 4.09E−02 −0.43 1.3hsa-asg- 10.13 9.79 9.88 9.88 9.92 0.11 100 1.15E−02 −0.43 1.3 5749hsa-asg- 9.42 9.34 9.11 9.38 9.28 0.15 100 1.85E−02 −0.43 1.4 8631hsa-asg- 9.57 9.28 9.24 9.59 9.34 0.17 14 1.23E−02 −0.44 1.4 7051hsa-asg- 6.02 6.00 6.23 6.30 6.08 0.17 57 4.92E−02 −0.45 1.4 10506hsa-asg-507 3.27 2.78 3.19 3.28 3.05 0.21 0 2.51E−02 −0.45 1.4 hsa-asg-8.67 8.16 8.33 8.40 8.26 0.23 100 3.98E−02 −0.45 1.4 6018 hsa-asg- 9.729.42 9.46 9.83 9.57 0.18 57 1.33E−02 −0.46 1.4 3240 hsa-asg-504 9.058.87 8.78 8.93 8.86 0.13 100 3.83E−03 −0.46 1.4 hsa-cand110 11.04 10.6810.64 10.96 10.78 0.16 100 6.77E−03 −0.46 1.4 hsa-asg- 11.62 11.80 11.1211.86 11.54 0.25 100 4.83E−02 −0.46 1.4 3811 hsa-asg- 11.11 10.76 11.1910.96 10.95 0.17 100 2.69E−02 −0.46 1.4 6398 hsa-asg- 10.13 9.69 9.9610.03 9.91 0.17 100 1.04E−02 −0.47 1.4 4731 hsa-asg- 10.72 10.41 10.6110.73 10.55 0.18 100 1.28E−02 −0.47 1.4 7096 hsa-asg- 9.63 9.31 9.419.41 9.34 0.18 100 9.17E−03 −0.48 1.4 6367 hsa-asg- 9.86 9.46 9.58 9.949.62 0.22 86 3.31E−02 −0.48 1.4 3456 hsa-asg- 7.00 6.75 6.70 6.84 6.730.21 100 1.84E−02 −0.49 1.4 1641 hsa-asg- 10.38 9.87 10.29 10.21 10.110.21 100 2.78E−02 −0.49 1.4 2980 hsa-asg- 9.10 8.69 8.59 8.97 8.83 0.19100 2.43E−02 −0.49 1.4 4828 hsa-asg- 9.49 9.23 9.02 9.46 9.28 0.18 1008.39E−03 −0.50 1.4 9266 hsa-asg- 11.05 10.69 10.91 10.90 10.82 0.14 1002.61E−03 −0.50 1.4 3260 hsa-asg- 8.46 8.25 8.00 8.43 8.22 0.21 1001.97E−02 −0.50 1.4 6802 hsa-asg- 5.59 6.00 6.20 6.35 5.99 0.27 294.20E−02 −0.51 1.4 7579 hsa-asg- 4.09 4.56 4.64 4.00 4.35 0.28 574.99E−02 −0.51 1.4 12857 hsa-asg- 8.72 8.73 8.52 8.76 8.51 0.24 1002.36E−02 −0.51 1.4 8421 hsa-cand583 6.19 6.06 5.90 5.74 5.94 0.22 292.82E−02 −0.52 1.4 hsa-asg- 9.41 9.05 9.07 9.35 9.18 0.18 100 1.04E−02−0.52 1.4 1123 hsa-asg- 9.57 9.19 9.93 9.42 9.47 0.23 100 3.53E−02 −0.521.4 11946 hsa-asg- 7.69 7.23 7.02 7.15 7.24 0.24 100 3.03E−02 −0.52 1.412953 hsa-asg- 8.98 8.33 8.45 8.74 8.51 0.27 100 4.69E−02 −0.52 1.410639 hsa-asg- 8.33 8.27 8.20 8.53 8.19 0.23 100 2.70E−02 −0.53 1.4 3345hsa-asg- 7.86 7.83 7.38 8.02 7.76 0.20 100 9.44E−03 −0.53 1.4 3230hsa-cand441 7.87 7.60 7.33 7.86 7.58 0.26 29 4.16E−02 −0.53 1.4 hsa-asg-10.74 10.45 10.91 10.81 10.63 0.20 100 2.06E−02 −0.53 1.4 3892 hsa-asg-7.27 6.84 7.04 7.41 7.12 0.18 100 8.27E−03 −0.53 1.4 7326 hsa-asg- 7.417.56 7.22 7.87 7.46 0.23 100 2.38E−02 −0.54 1.5 1174 hsa-asg- 6.85 7.016.85 7.40 7.12 0.29 14 4.14E−02 −0.54 1.5 3290 hsa-asg- 8.55 8.31 7.928.30 8.26 0.20 100 1.76E−02 −0.55 1.5 12441 hsa-asg- 8.20 7.85 7.40 8.127.78 0.32 100 4.82E−02 −0.56 1.5 4502 hsa-asg- 9.63 9.10 9.53 9.42 9.400.17 14 3.42E−03 −0.56 1.5 11758 hsa-asg- 7.26 7.29 6.83 7.51 7.15 0.26100 2.84E−02 −0.57 1.5 4759 hsa-asg- 7.92 7.56 7.86 7.91 7.66 0.24 431.68E−02 −0.57 1.5 3808 hsa-asg- 8.64 8.11 7.94 8.42 8.23 0.26 1002.15E−02 −0.57 1.5 11249 hsa-asg- 10.02 9.77 9.75 9.91 9.75 0.19 1009.17E−03 −0.58 1.5 6728 hsa-asg- 8.03 7.89 7.42 8.11 7.81 0.23 1003.67E−02 −0.58 1.5 2891 hsa-asg- 4.93 5.14 5.55 5.05 5.01 0.28 294.49E−02 −0.58 1.5 14186 hsa-cand735 8.20 7.89 8.31 8.15 8.06 0.16 1001.68E−03 −0.58 1.5 hsa-asg- 5.43 5.59 5.28 5.81 5.58 0.19 14 4.99E−03−0.58 1.5 8391 hsa-asg- 6.94 6.75 6.10 6.71 6.56 0.28 100 2.92E−02 −0.581.5 1617 hsa-asg- 8.86 8.60 8.49 8.91 8.70 0.17 100 2.38E−03 −0.59 1.52626 hsa-asg- 7.19 6.83 6.67 7.47 6.95 0.28 100 3.91E−02 −0.59 1.5 11252hsa-asg- 5.00 5.06 4.81 5.29 5.21 0.32 29 4.64E−02 −0.59 1.5 11585hsa-asg- 7.79 7.31 7.36 7.82 7.61 0.21 100 8.13E−03 −0.59 1.5 10575hsa-asg- 7.73 7.44 7.29 7.69 7.40 0.23 100 1.02E−02 −0.59 1.5 14066hsa-asg- 8.72 8.36 8.81 8.90 8.52 0.28 100 2.43E−02 −0.60 1.5 11729hsa-asg- 8.00 8.25 7.58 7.74 7.80 0.27 100 3.23E−02 −0.60 1.5 11521hsa-asg- 9.05 8.72 8.96 9.05 8.94 0.14 100 8.23E−03 −0.61 1.5 12581hsa-asg- 9.73 9.22 9.47 9.31 9.38 0.18 29 2.49E−03 −0.61 1.5 13079hsa-asg- 5.05 5.54 4.90 5.62 5.30 0.28 14 3.63E−02 −0.61 1.5 7569hsa-asg- 9.22 8.86 8.71 8.98 8.94 0.19 100 9.37E−03 −0.62 1.5 3462hsa-asg- 7.07 6.84 6.93 7.25 7.00 0.27 100 4.53E−02 −0.62 1.5 8227hsa-asg-508 6.71 6.64 6.35 6.92 6.48 0.31 86 3.32E−02 −0.62 1.5 hsa-asg-7.97 7.72 7.47 8.34 7.78 0.29 100 4.94E−02 −0.62 1.5 2580 hsa-asg- 7.647.68 7.56 7.85 7.60 0.28 100 2.31E−02 −0.63 1.5 7687 hsa-asg-688 7.917.40 8.14 7.54 7.69 0.25 100 1.49E−02 −0.63 1.5 hsa-asg- 8.25 8.21 7.688.28 8.02 0.28 100 1.79E−02 −0.63 1.6 3746 hsa-asg- 5.68 5.33 5.66 5.955.54 0.30 14 4.42E−02 −0.64 1.6 2041 hsa-asg-819 8.17 7.75 7.65 8.077.76 0.32 100 4.78E−02 −0.64 1.6 hsa-asg- 5.08 5.45 4.66 5.07 5.02 0.270 1.64E−02 −0.64 1.6 4200 hsa-asg- 6.76 7.17 7.08 7.45 7.19 0.26 1002.23E−02 −0.64 1.6 4469 hsa-asg- 5.29 5.04 5.02 5.67 5.16 0.30 292.09E−02 −0.65 1.6 10183 hsa-cand523 6.46 6.15 6.12 6.18 6.11 0.22 435.92E−03 −0.65 1.6 hsa-asg- 6.44 6.76 6.28 6.57 6.42 0.24 43 3.54E−02−0.65 1.6 8393 hsa-asg- 6.37 6.69 6.70 6.41 6.66 0.21 100 4.15E−03 −0.661.6 9729 hsa-asg- 6.70 6.46 6.33 6.83 6.59 0.18 43 6.64E−03 −0.66 1.66675 hsa-asg- 8.37 8.11 8.23 8.18 8.11 0.22 100 4.42E−03 −0.66 1.6 1813hsa-asg- 6.57 6.24 5.98 6.75 6.38 0.24 71 8.03E−03 −0.67 1.6 8562hsa-asg- 7.46 7.71 7.43 7.38 7.45 0.35 100 3.60E−02 −0.67 1.6 11479hsa-asg- 9.64 9.49 8.79 9.50 9.22 0.32 14 2.54E−02 −0.67 1.6 5379hsa-asg- 6.34 6.16 6.25 6.45 6.24 0.30 14 1.80E−02 −0.68 1.6 11563hsa-asg- 7.55 7.05 6.97 7.26 7.20 0.18 100 2.32E−03 −0.68 1.6 2131hsa-asg- 6.87 6.46 6.44 6.87 6.71 0.22 100 1.89E−02 −0.69 1.6 5289hsa-asg- 6.47 6.45 6.25 6.98 6.63 0.31 43 2.33E−02 −0.69 1.6 8565hsa-asg- 6.09 5.92 5.78 5.65 5.76 0.20 57 2.21E−03 −0.69 1.6 4599hsa-asg- 6.26 5.77 5.83 6.61 6.12 0.39 29 4.43E−02 −0.70 1.6 3340hsa-asg- 6.56 6.17 6.75 6.27 6.39 0.20 71 7.84E−03 −0.70 1.6 3694hsa-asg- 12.36 12.02 12.29 12.59 12.37 0.22 100 9.45E−03 −0.70 1.6 9530hsa-asg- 8.21 8.11 7.37 8.26 7.95 0.34 100 3.43E−02 −0.70 1.6 5085hsa-asg- 6.83 6.56 7.22 6.82 6.85 0.24 71 7.23E−03 −0.71 1.6 2588hsa-asg- 6.93 7.18 6.82 7.21 7.03 0.18 100 2.20E−03 −0.71 1.6 5061hsa-asg- 8.41 8.05 7.85 8.36 8.13 0.24 100 4.88E−03 −0.72 1.6 11155hsa-asg- 3.97 4.10 4.24 3.72 4.01 0.17 14 2.90E−03 −0.72 1.6 8672hsa-asg- 7.38 7.25 6.46 7.11 7.00 0.33 100 3.40E−02 −0.72 1.7 1576hsa-asg- 6.62 6.35 6.43 6.59 6.31 0.37 29 4.16E−02 −0.72 1.7 7730hsa-asg- 9.92 9.61 9.48 9.80 9.78 0.19 86 4.74E−03 −0.73 1.7 12247hsa-asg- 7.72 7.52 7.04 7.49 7.25 0.33 100 2.01E−02 −0.73 1.7 9511hsa-asg- 6.41 6.43 5.55 5.73 6.02 0.33 71 2.24E−02 −0.73 1.7 8959hsa-asg-797 5.43 5.38 5.14 5.48 5.32 0.14 14 5.40E−04 −0.73 1.7hsa-asg-271 4.53 4.56 4.21 4.91 4.46 0.25 14 5.85E−03 −0.73 1.7 hsa-asg-11.61 11.16 11.31 11.87 11.49 0.27 100 3.14E−02 −0.73 1.7 2750 hsa-asg-5.84 6.63 6.66 6.01 6.31 0.34 86 3.14E−02 −0.74 1.7 2693 hsa-asg-5310.14 9.70 9.68 10.04 9.85 0.17 100 9.81E−04 −0.74 1.7 hsa-asg- 9.529.33 9.65 9.45 9.37 0.19 100 1.20E−02 −0.74 1.7 5032 hsa-asg- 7.51 7.237.06 7.41 7.25 0.16 100 3.18E−03 −0.74 1.7 6892 hsa-asg- 7.79 6.85 7.006.75 6.99 0.38 100 3.64E−02 −0.75 1.7 1911 hsa-asg- 7.13 6.79 6.72 7.046.73 0.33 86 4.95E−02 −0.75 1.7 8675 hsa-asg-809 6.51 6.49 5.76 6.386.18 0.35 57 2.63E−02 −0.75 1.7 hsa-asg- 8.03 7.57 7.81 8.30 7.87 0.25100 2.50E−02 −0.75 1.7 11893 hsa-asg- 8.18 8.03 7.48 8.27 7.93 0.28 1004.33E−02 −0.75 1.7 1861 hsa-asg- 6.00 6.13 6.08 6.14 6.19 0.24 1003.52E−03 −0.77 1.7 6651 hsa-asg-471 8.22 8.20 8.26 8.17 8.18 0.07 1002.36E−05 −0.77 1.7 hsa-asg- 5.59 6.32 5.55 5.65 5.92 0.40 14 3.65E−02−0.77 1.7 10861 hsa-asg- 8.44 8.83 8.12 8.89 8.53 0.28 100 8.08E−03−0.78 1.7 4310 hsa-asg- 7.97 8.26 7.75 8.05 8.06 0.41 100 3.48E−02 −0.801.7 7153 hsa-asg- 5.48 5.71 5.49 6.18 5.74 0.26 43 8.95E−03 −0.81 1.86879 hsa-asg- 5.90 5.88 5.66 6.63 6.04 0.33 14 1.45E−02 −0.81 1.8 7352hsa-asg- 7.54 7.35 7.24 7.28 7.45 0.25 100 3.40E−03 −0.82 1.8 12775hsa-asg- 8.06 8.07 7.04 8.28 7.91 0.40 100 3.40E−02 −0.83 1.8 13613hsa-asg- 2.27 2.59 2.75 3.39 2.75 0.40 0 3.92E−02 −0.83 1.8 9748hsa-asg- 6.88 7.06 6.84 7.05 6.89 0.13 100 1.29E−03 −0.83 1.8 7640hsa-asg- 7.41 6.54 6.90 7.16 6.90 0.31 100 1.01E−02 −0.84 1.8 10971hsa-asg- 9.64 8.99 9.85 9.19 9.33 0.33 14 1.95E−02 −0.84 1.8 6092hsa-asg-813 5.40 5.18 4.90 6.22 5.47 0.43 14 3.47E−02 −0.84 1.8 hsa-asg-7.79 7.54 7.17 7.98 7.60 0.27 100 3.99E−03 −0.84 1.8 6437 hsa-asg- 8.508.10 8.12 8.15 8.27 0.17 100 2.71E−04 −0.84 1.8 12946 hsa-asg- 8.90 8.288.40 8.89 8.53 0.26 100 3.02E−03 −0.85 1.8 6948 hsa-asg- 7.97 7.23 7.518.28 7.68 0.39 43 2.85E−02 −0.86 1.8 11486 hsa-cand711 10.23 9.89 9.9010.09 9.98 0.14 100 6.98E−05 −0.86 1.8 hsa-cand588 6.47 6.23 6.52 7.436.66 0.45 86 3.57E−02 −0.88 1.8 hsa-asg-555 7.31 7.21 7.52 7.62 7.210.34 100 2.71E−02 −0.88 1.8 hsa-asg- 7.73 7.47 8.56 8.09 7.80 0.41 432.37E−02 −0.88 1.8 6149 hsa-asg- 8.09 7.91 7.21 8.35 7.76 0.40 433.83E−02 −0.88 1.8 9920 hsa-asg- 7.44 6.84 6.84 7.56 7.16 0.28 1001.60E−02 −0.88 1.8 1391 hsa-asg- 7.39 7.38 6.75 7.57 7.06 0.41 1002.63E−02 −0.88 1.8 3150 hsa-asg- 7.02 7.19 6.25 7.30 7.00 0.42 863.49E−02 −0.88 1.8 13829 hsa-asg- 7.26 7.18 6.44 7.26 6.79 0.48 864.66E−02 −0.88 1.8 9382 hsa-cand855 7.29 6.56 6.86 6.91 6.79 0.34 711.42E−02 −0.88 1.8 hsa-asg- 8.40 7.98 7.22 8.52 7.99 0.42 100 2.42E−02−0.89 1.9 10716 hsa-asg- 9.09 8.81 8.38 8.97 8.80 0.27 100 3.11E−03−0.90 1.9 11698 hsa-cand902 6.04 6.44 5.81 6.41 6.09 0.33 29 8.47E−03−0.90 1.9 hsa-asg- 9.36 8.84 8.89 9.14 9.16 0.24 100 1.60E−03 −0.92 1.912919 hsa-asg- 5.97 4.73 5.44 5.66 5.34 0.42 14 2.03E−02 −0.92 1.9 4261hsa-asg- 7.20 7.05 7.54 7.10 7.22 0.16 100 1.72E−04 −0.92 1.9 1153hsa-asg- 6.37 6.30 6.16 6.91 6.43 0.28 71 1.75E−02 −0.92 1.9 3841hsa-asg- 11.47 11.22 11.49 11.61 11.44 0.27 100 6.61E−03 −0.92 1.9 3231hsa-asg-225 5.62 5.40 5.81 6.06 5.48 0.36 14 1.67E−02 −0.93 1.9 hsa-asg-8.14 8.20 7.51 8.38 7.92 0.34 100 1.26E−02 −0.93 1.9 2017 hsa-asg- 7.597.31 7.03 8.04 7.33 0.37 100 1.23E−02 −0.93 1.9 6623 hsa-asg- 6.20 5.865.96 5.75 5.83 0.21 29 6.69E−04 −0.93 1.9 9526 hsa-asg- 6.45 5.24 5.815.74 5.88 0.48 14 3.57E−02 −0.93 1.9 3014 hsa-asg- 7.36 8.29 7.94 8.007.93 0.48 100 3.31E−02 −0.94 1.9 12957 hsa-asg- 4.62 5.01 4.00 4.81 4.760.38 14 1.68E−02 −0.95 1.9 2564 hsa-asg- 5.84 5.33 5.78 5.92 5.75 0.2029 5.26E−03 −0.96 2.0 10473 hsa-asg- 4.09 4.14 3.68 4.20 3.75 0.43 02.32E−02 −0.97 2.0 8303 hsa-asg- 5.58 6.30 6.32 6.18 6.26 0.40 1001.94E−02 −0.97 2.0 11310 hsa-asg- 7.03 6.70 6.68 6.74 6.83 0.33 1005.73E−03 −0.97 2.0 6831 hsa-asg- 7.41 8.23 7.88 7.80 7.91 0.49 713.60E−02 −0.97 2.0 11373 hsa-asg- 6.69 7.48 7.00 7.31 7.10 0.33 1004.64E−03 −1.00 2.0 6005 hsa-asg- 10.16 9.78 9.73 9.76 9.73 0.22 861.76E−03 −1.01 2.0 4654 hsa-asg- 8.59 8.50 8.40 8.84 8.55 0.42 1001.56E−02 −1.01 2.0 9403 hsa-asg- 9.08 8.61 8.72 8.94 8.71 0.30 1002.51E−03 −1.01 2.0 7893 hsa-asg- 7.76 8.15 9.01 8.03 8.19 0.51 1003.54E−02 −1.05 2.1 13000 hsa-asg- 6.34 7.03 7.09 7.35 6.98 0.47 1002.02E−02 −1.05 2.1 10883 hsa-asg- 6.19 6.11 6.37 6.57 6.12 0.27 293.24E−03 −1.06 2.1 1689 hsa-asg- 7.21 7.45 6.33 7.66 7.14 0.52 714.62E−02 −1.06 2.1 9412 hsa-asg- 9.06 8.59 9.13 8.71 8.73 0.26 1002.57E−03 −1.07 2.1 3271 hsa-asg- 7.92 7.41 6.44 7.75 7.41 0.49 863.10E−02 −1.08 2.1 13559 hsa-asg- 4.77 4.75 5.31 5.52 4.81 0.45 141.38E−02 −1.09 2.1 11898 hsa-asg- 4.15 4.90 4.39 5.46 4.53 0.49 01.97E−02 −1.10 2.1 3081 hsa-asg- 7.29 6.62 6.20 7.41 6.82 0.42 712.08E−02 −1.10 2.1 2858 hsa-asg-278 6.33 6.52 5.76 6.70 6.08 0.55 433.82E−02 −1.11 2.2 hsa-asg- 9.23 9.06 8.97 9.07 8.91 0.23 100 4.65E−04−1.11 2.2 5423 hsa-asg- 5.08 5.77 5.66 5.90 5.58 0.31 29 2.50E−03 −1.112.2 9257 hsa-asg- 7.31 6.58 6.82 7.81 7.05 0.48 100 1.85E−02 −1.12 2.210197 hsa-asg- 8.49 7.73 7.72 8.15 8.13 0.32 100 2.69E−03 −1.14 2.2 6507hsa-asg- 6.64 6.24 6.27 7.16 6.62 0.33 86 5.68E−03 −1.14 2.2 9015hsa-asg- 3.00 3.03 3.30 2.47 3.00 0.32 0 2.23E−03 −1.15 2.2 5460hsa-asg- 10.06 10.04 9.91 10.57 10.19 0.44 100 9.65E−03 −1.16 2.2 13308hsa-asg- 6.30 5.56 5.28 6.05 5.79 0.34 29 5.93E−03 −1.16 2.2 4440hsa-asg- 7.26 7.20 6.71 7.54 7.13 0.26 100 3.42E−03 −1.16 2.2 1375hsa-asg- 7.11 6.74 6.28 6.97 6.74 0.31 71 1.16E−02 −1.17 2.3 8763hsa-asg- 8.90 10.21 9.61 9.90 9.76 0.54 100 2.17E−02 −1.19 2.3 2872hsa-asg- 7.41 8.16 7.48 7.69 7.83 0.42 100 6.30E−03 −1.20 2.3 12460hsa-asg- 6.64 6.59 6.28 6.91 6.44 0.40 29 5.62E−03 −1.21 2.3 8138hsa-asg- 10.21 10.17 10.09 10.64 10.31 0.38 100 3.56E−03 −1.21 2.3 5304hsa-asg- 6.63 6.25 6.49 6.79 6.41 0.26 14 1.14E−02 −1.21 2.3 4971hsa-asg- 7.85 7.27 7.45 7.45 7.38 0.25 100 6.72E−04 −1.23 2.4 9343hsa-asg- 6.19 6.52 6.21 6.96 6.36 0.31 71 1.00E−03 −1.24 2.4 8271hsa-asg- 7.70 8.18 8.07 8.66 8.30 0.35 100 4.90E−03 −1.25 2.4 13260hsa-asg- 6.17 6.19 5.63 4.94 5.54 0.62 14 2.98E−02 −1.26 2.4 12105hsa-asg- 4.21 4.68 3.03 4.10 4.06 0.66 29 4.45E−02 −1.26 2.4 7685hsa-asg- 6.53 6.61 7.48 7.28 6.94 0.42 100 4.87E−03 −1.27 2.4 3360hsa-asg- 5.45 5.95 6.49 6.27 6.06 0.49 57 2.32E−02 −1.28 2.4 5576hsa-asg- 6.34 5.88 5.40 6.34 5.94 0.40 29 1.37E−02 −1.29 2.4 4843hsa-asg- 5.77 6.28 5.90 6.18 6.17 0.49 29 1.41E−02 −1.29 2.4 7009hsa-asg- 4.98 4.83 5.34 4.76 5.04 0.29 0 7.84E−03 −1.30 2.5 8928hsa-asg- 5.90 5.78 5.55 6.29 5.67 0.38 14 4.72E−03 −1.32 2.5 12777hsa-asg- 5.29 6.38 6.35 6.54 6.27 0.59 43 2.15E−02 −1.35 2.6 7069hsa-cand965 5.89 7.57 7.23 7.51 7.29 0.66 29 4.98E−02 −1.38 2.6 hsa-asg-5.92 5.95 6.08 6.24 6.06 0.18 43 1.88E−03 −1.38 2.6 13693 hsa-asg- 6.406.00 5.14 6.55 6.05 0.45 43 7.16E−03 −1.40 2.6 8329 hsa-asg- 7.21 6.856.38 7.46 6.74 0.52 71 1.05E−02 −1.43 2.7 3597 hsa-asg- 5.99 6.19 6.055.82 6.05 0.30 57 6.21E−03 −1.44 2.7 9852 hsa-asg- 4.29 5.06 4.45 4.184.41 0.34 0 1.68E−03 −1.45 2.7 3107 hsa-asg- 12.09 11.62 12.09 12.3412.07 0.52 100 7.47E−03 −1.47 2.8 14245 hsa-asg- 5.55 6.37 5.92 6.276.05 0.30 29 1.95E−03 −1.47 2.8 6949 hsa-asg- 5.51 5.67 5.34 5.86 5.540.20 57 2.22E−04 −1.52 2.9 7319 hsa-asg- 6.32 6.22 5.63 6.72 6.30 0.4543 7.44E−03 −1.52 2.9 9022 hsa-asg- 6.52 6.80 5.94 6.66 6.40 0.34 715.08E−04 −1.56 2.9 12246 hsa-asg- 7.13 7.51 7.37 7.66 7.46 0.56 866.76E−03 −1.58 3.0 8917 hsa-asg- 6.60 6.87 6.20 6.42 6.85 0.51 1005.18E−03 −1.60 3.0 13244 hsa-cand345 6.17 6.16 5.85 6.83 6.10 0.41 711.58E−03 −1.61 3.0 hsa-asg- 5.99 7.07 7.51 7.63 7.32 0.78 100 4.16E−02−1.62 3.1 12702 hsa-asg- 6.78 6.78 6.67 6.76 6.84 0.17 100 2.98E−04−1.67 3.2 13230 hsa-asg- 6.74 6.97 5.90 6.60 6.84 0.58 100 1.10E−02−1.69 3.2 12902 hsa-asg- 6.50 7.57 6.95 7.53 7.17 0.39 100 2.48E−03−1.71 3.3 13237 hsa-asg- 6.01 7.72 7.23 7.51 7.13 0.85 86 2.80E−02 −1.753.4 4002 hsa-asg-21 4.64 6.59 6.30 6.40 6.05 0.75 86 3.18E−02 −1.77 3.4hsa-asg- 6.80 7.78 7.50 7.81 7.62 0.48 86 2.61E−03 −1.79 3.5 10149hsa-asg- 7.20 7.17 6.77 6.40 6.97 0.53 100 2.85E−02 −1.83 3.6 5303hsa-asg- 4.64 6.80 5.76 6.43 6.24 0.83 71 4.84E−02 −1.84 3.6 10666hsa-asg-335 6.22 8.78 7.94 8.01 7.82 1.01 100 4.11E−02 −1.88 3.7hsa-asg- 5.40 7.00 6.32 6.33 6.21 0.61 29 3.93E−03 −1.98 3.9 6250hsa-asg- 4.84 6.07 5.90 7.15 6.08 0.78 29 1.18E−02 −1.98 4.0 8208hsa-asg- 6.14 8.68 8.01 8.23 7.78 1.09 100 4.01E−02 −2.04 4.1 10387hsa-asg- 9.18 8.89 8.86 10.09 9.20 0.58 100 1.86E−03 −2.09 4.2 13248hsa-asg- 4.58 6.46 6.44 5.31 5.67 0.87 71 2.27E−02 −2.12 4.4 12079hsa-asg- 7.60 8.20 7.32 7.98 7.57 0.40 100 5.68E−04 −2.13 4.4 4557hsa-asg- 8.05 8.75 8.88 9.64 8.92 0.62 100 1.81E−02 −2.17 4.5 13937hsa-miR165 6.70 5.83 5.14 5.80 5.55 0.63 14 7.52E−03 −2.18 4.5 hsa-asg-5.81 6.86 6.68 7.27 6.65 0.96 86 1.84E−02 −2.24 4.7 8682 hsa-asg- 4.736.11 5.83 5.74 5.71 0.61 29 4.97E−03 −2.27 4.8 6413 hsa-asg- 6.29 6.826.28 7.15 6.60 0.42 86 1.45E−03 −2.29 4.9 13304 hsa-asg- 4.46 6.75 6.286.63 5.93 1.04 71 3.02E−02 −2.29 4.9 4205 hsa-asg-870 5.18 6.42 6.056.50 6.12 1.05 43 1.74E−02 −2.44 5.4 hsa-asg- 9.80 10.34 10.32 10.7610.27 0.34 100 1.37E−03 −2.46 5.5 7074 hsa-asg- 7.36 7.25 6.90 7.66 7.420.37 100 9.08E−03 −2.48 5.6 13279 hsa-miR- 10.97 10.63 10.28 11.16 10.880.36 100 5.79E−03 −1.24 2.4 29b hsa-miR-9 3.74 3.48 4.00 4.74 3.68 0.620 2.64E−02 2.10 4.3 hsa-miR- 7.54 6.20 6.64 7.66 6.81 0.63 86 7.95E−031.73 3.3 421 SD, standard deviation; %, percentage of samples expressingmiRNA above background levels; Log2 Diff, difference in VSN-transformedexpression between MEL and LNneg samples.

Example 11 miRNAs Differentially Expressed between Cancer-positive andCancer-negative Lymph Nodes from Melanoma Patients

To identify miRNAs that are useful markers for metastasis of melanoma tolymph nodes, the inventors identified miRNAs that are expressed atsignificantly higher levels in cancer-positive lymph nodes (LNpos,frozen biopsy specimens, Table 9) than in cancer-negative lymph nodes(LNneg1-7, frozen biopsy specimens, Table 9), from melanoma patients.miRNAs were purified and expression was analyzed with microarrays asdescribed above in Example 2. miRNA expression profiles were establishedfor three positive lymph nodes (LNpos1A, LNpos2B, LNpos3B) and comparedwith expression profiles from the seven cancer-negative lymph nodes(LNneg1-7) and expression profiles from two melanoma (MEL1, MEL3)samples. Pearson correlation analyses indicated that the correlationbetween arrays was higher between the LNpos and the MEL samples thanbetween the LNpos and LNneg samples (data not shown). Further analysisalso revealed significant differences between the miRNA expressionprofiles of one positive lymph node (LNpos1A) and those of the two otherpositive lymph nodes (LNpos2B and 3B) for a subset of miRNAs (data notshown). Therefore, data were reanalyzed with the positive lymph nodesseparated into two distinct groups for the analysis (LNposA for LNpos1Aand LNposB for LNpos2B and 3B). Under these new conditions, hierarchicalclustering analysis, as well as principal component analysis on thesignificantly differentially expressed miRNAs between the four differentgroups of samples confirmed the heterogeneity between LNposA and LNposB(FIG. 6). The existence of two subgroups within the positive lymph nodesamples was confirmed by further real time RT-PCR analysis. The LNpos1Asample was from a patient with a primary melanoma and regionalmetastasis, but no distant metastasis. The melanoma was invasive throughthe reticular dermis into the subcutaneous tissue (Clark's level V), andseven of eight lymph nodes from the patient were cancer-positive. TheLNpos3B sample was from a patient with recurrent melanoma, and theLNpos2B sample was from a patient with a cancer of unknown primaryorigin (CUP). As the histopathological reports of the primary tumorswere very similar in all cases (Table 9), these data suggest thatrecurrent melanoma and CUP differ from first-step metastatic tumors atthe molecular level.

As a consequence of the heterogeneity within the positive lymph nodes,comparisons of miRNA expression between cancer-positive andcancer-negative lymph nodes were made separately with the two LNpossubgroups, LNposA (Table 11) and LNposB (Table 12).

Seventy miRNAs (36 verified and 34 predicted) were determined to havesignificantly higher average expression levels in LNpos1A sample ascompared to their average expression levels in LNneg samples (log 2-Diff(LNpos1A vs LNneg)≧1 and t-test (LNpos1A vs LNneg)<0.05) (Table 11).Among the verified miRNAs, hsa-miR-211 and -375 were expressed between90- and 140-fold higher in LNpos1A than in LNneg; seven miRNAs(hsa-miR-203, -200b, -200c, -509, -513, -429, and -767-5p) wereexpressed at levels between 20- and 50-fold higher in LNpos1A than inLNneg; and 15 miRNAs had average expression levels between 5- and20-fold higher in LNpos1A compared to LNneg (Table 11). In addition, 14miRNAs had average expression levels between 2- and 5-fold lower inLNpos1A than in LNneg. Among the predicted miRNAs, three(hsa-asg-5638_st2, -1296_st1, -7637_st2) were expressed at levels atleast 10-fold higher in LNpos1A than in LNneg; and four miRNAs(hsa-asg-4803_st2, -11338_st2, -1519_st1, and -14208-st1) were expressedat levels between 5- and 10-fold higher in LNpos1A than in LNneg samples(Table 11).

TABLE 11 MicroRNAs Differentially Expressed Between Cancer-PositiveLymph Node Group A Sample (LNpos1A) and Cancer-Negative Lymph NodeSamples (LNneg1-7) from Melanoma Patients. Probe LNpos LNneg MicroRNA ID1A % 1 2 3 4 5 6 7 hsa-miR-211 hsa-miR-211_st1 11.1 100 3.20 5.75 2.624.64 5.22 4.00 3.05 hsa-miR-375 hsa-miR-375_st1 10.7 100 3.87 5.12 3.264.79 4.10 3.84 4.87 hsa-miR-203 hsa-miR-203_st2 11.4 100 4.37 5.90 5.845.94 5.88 6.58 6.38 hsa-miR-200b has-miR-200b_st1 12.4 100 6.34 6.106.86 7.11 6.78 7.40 8.10 hsa-miR-200c hsa-miR-200c_st1 13.1 100 8.907.29 7.04 8.69 6.85 7.87 7.98 hsa-miR-509 hsa-miR-509_st2 11.6 100 6.098.04 6.00 5.45 7.04 6.25 6.22 hsa-miR-513 hsa-miR-513_st1 10.2 100 3.017.12 5.52 5.21 5.40 5.47 5.75 hsa-miR-429 hsa-miR-429_st1 7.46 100 3.013.06 2.62 3.00 2.66 2.86 3.62 hsa-miR-767-5p hsa-asg-558_st1 7.44 1002.69 3.70 2.76 2.76 2.66 2.40 3.81 hsa-miR-187 hsa-miR-187_st1 7.81 1002.90 2.82 4.01 4.42 3.94 3.49 4.15 hsa-miR-592 hsa-miR-592_st1 6.89 1002.80 3.40 3.14 3.52 2.27 2.51 3.28 hsa-miR-204 hsa-miR-204_st2 9.77 1003.80 6.02 5.29 7.03 6.43 6.10 6.45 hsa-miR-105 hsa-miR-105_st1 6.86 1003.01 3.29 3.59 3.63 2.78 4.27 4.27 hsa-miR-132 hsa-miR-132_st1 8.84 1006.62 4.36 4.55 7.38 5.36 4.76 5.89 hsa-miR-200a hsa-miR-200a_st2 8.76100 4.84 5.36 4.88 6.08 5.59 5.31 6.40 hsa-miR-605 hsa-miR-605_st1 6.32100 3.48 3.06 2.95 3.05 2.34 3.40 3.31 hsa-miR-215 hsa-miR-215_st1 9.98100 6.29 6.90 6.91 6.94 6.44 7.29 8.01 hsa-miR-183 hsa-miR-183_st1 9.26100 5.47 6.35 6.90 6.94 6.34 6.12 6.04 hsa-miR-196b hsa-miR-196b_st210.0 100 6.69 7.00 6.23 9.13 7.24 6.67 7.32 hsa-miR-508 hsa-miR-508_st19.50 100 6.06 7.31 7.14 6.49 6.65 6.49 6.15 hsa-miR-493-5phsa-miR-493-5p_st1 7.10 100 3.64 3.70 3.26 4.75 3.77 5.49 5.40hsa-miR-194 hsa-miR-194_st2 12.2 100 8.75 9.43 9.39 9.35 9.29 10.3 10.5hsa-miR-421 hsa-miR-421-3p_st1 10.08 100 7.78 6.55 6.63 8.46 7.14 7.298.29 hsa-miR-192 hsa-miR-192_st2 13.1 100 10.2 10.1 10.6 10.2 10.0 11.311.5 hsa-miR-1 hsa-miR-1_st1 11.8 100 8.27 9.62 8.72 10.7 10.1 9.58 10.0hsa-miR-506 hsa-miR-506_st2 8.70 100 6.15 7.11 6.59 6.01 6.77 6.66 6.08hsa-miR-584 hsa-miR-584_st2 7.67 100 4.80 5.52 5.44 5.12 5.71 5.61 6.12hsa-miR-522 hsa-miR-522_st2 6.53 100 4.48 3.96 3.59 5.07 4.44 5.17 3.62hsa-miR-510 hsa-miR-510_st2 7.15 100 4.62 5.83 5.85 4.88 5.04 4.33 4.66hsa-miR-576 hsa-miR-576_st2 4.91 0 2.80 2.44 3.26 3.18 2.91 2.28 3.20hsa-miR-495 hsa-miR-495_st1 6.77 100 4.26 4.84 4.28 5.04 4.92 5.44 5.38hsa-miR-182 hsa-miR-182_st1 10.3 100 7.90 8.91 8.60 9.24 8.03 8.90 8.80hsa-miR-514 hsa-miR-514_st1 4.58 0 3.01 3.06 2.89 2.91 3.03 2.98 3.05hsa-miR-450 hsa-miR-450_st2 5.41 0 3.30 4.39 3.02 4.49 3.86 3.59 4.32hsa-miR-382 hsa-miR-382_st1 7.90 100 6.09 5.99 6.13 7.03 6.42 6.79 7.17hsa-miR-10a hsa-miR-10a_st1 12.1 100 10.6 11.0 10.6 11.5 11.3 11.0 11.5hsa-miR-30d hsa-miR-30d_st1 13.1 100 11.8 12.3 12.2 12.4 12.2 12.4 12.6hsa-miR-30a-5p hsa-miR-30a-5p_st2 12.4 100 11.2 11.6 11.6 11.7 11.6 11.712.1 hsa-miR-27b hsa-miR-27b_st1 12.8 100 11.9 12.0 12.0 12.2 12.2 12.112.3 hsa-miR-23b hsa-miR-23b_st1 13.7 100 13.3 13.1 13.2 13.4 13.3 13.013.1 hsa-miR-21 hsa-miR-21_st2 13.7 100 13.1 13.6 13.2 13.2 13.2 13.213.2 hsa-let-7e hsa-let-7e_st1 12.6 100 12.0 12.2 12.1 12.2 12.3 12.012.2 hsa-miR-155 hsa-miR-155_st1 13.2 100 13.9 13.8 13.8 13.6 13.6 13.513.6 hsa-miR-195 hsa-miR-195_st2 12.1 100 12.7 13.0 12.8 12.9 13.0 12.612.8 hsa-miR-564 hsa-miR-564_st2 5.83 0 7.17 6.77 6.58 7.56 6.76 6.606.85 hsa-miR-34a hsa-miR-34a_st1 6.99 100 7.72 7.95 8.14 7.89 7.98 8.368.64 hsa-miR-22 hsa-miR-22_st1 10.3 100 10.6 11.6 11.7 11.2 11.7 11.811.9 hsa-miR-15a hsa-miR-15a_st1 10.4 100 12.1 11.7 11.4 11.6 11.2 11.512.1 hsa-miR-342 hsa-miR-342_st1 12.2 100 13.3 13.7 13.8 13.3 13.5 13.513.4 hsa-miR-186 hsa-miR-186_st1 6.33 100 7.38 7.96 7.26 7.76 7.96 7.438.71 hsa-miR-142-3p hsa-miR-142-3p_st2 3.34 0 5.20 5.14 5.11 4.97 4.785.06 6.15 hsa-miR-486 hsa-miR-486_st2 6.10 0 8.19 7.39 8.20 8.08 7.238.54 8.29 hsa-miR-29b hsa-miR-29b_st1 8.95 100 11.2 11.0 10.8 10.9 10.410.3 11.0 hsa-miR-29c hsa-miR-29c_st2 8.50 100 11.1 10.4 10.4 9.85 9.8210.2 10.9 hsa-miR-199a hsa-miR-199a_st2 8.21 100 9.52 10.1 10.1 10.110.4 10.3 10.5 hsa-miR-497 hsa-miR43_st1 5.55 0 7.19 7.68 7.66 7.25 7.917.69 7.88 hsa-miR-598 hsa-miR-598_st2 4.95 0 8.05 6.96 6.71 6.71 6.137.22 7.99 hsa-asg-5638 hsa-asg-5638_st2 8.00 100 3.20 5.22 3.02 2.953.48 3.59 3.49 hsa-asg-1296 hsa-asg-1296_st1 6.41 0 3.20 2.94 2.62 3.232.91 3.19 2.97 hsa-asg-7637 hsa-asg-7637_st2 7.25 100 3.56 3.79 3.694.32 3.68 3.76 4.32 hsa-asg-4803 hsa-asg-4803_st2 7.48 100 4.26 3.834.83 4.09 5.41 2.86 5.11 hsa-asg-11338 hsa-asg-11338_st2 6.79 100 4.323.88 3.54 3.70 4.47 3.68 4.15 hsa-asg-1519 hsa-asg-1519_st1 9.22 1005.74 6.92 7.20 6.03 7.32 6.38 5.77 hsa-asg-14208 hsa-asg-14208_st1 7.10100 3.80 3.70 4.39 4.21 3.98 5.14 5.46 hsa-asg-9514 hsa-asg-9514_st15.25 0 3.01 3.18 3.02 3.09 2.40 3.49 3.13 hsa-asg-247 hsa-asg-247_st17.54 100 5.17 5.48 5.27 5.52 4.95 5.40 5.66 hsa-asg-11361hsa-asg-11361_st2 5.44 100 2.90 3.18 3.38 3.81 3.48 3.49 3.28hsa-asg-1268 hsa-asg-1268_st1 6.20 100 4.08 3.96 3.20 4.51 4.06 4.814.92 hsa-asg-9980 hsa-asg-9980_st1 6.24 100 4.37 4.33 3.74 4.73 3.904.07 4.83 hsa-asg-9987 hsa-asg-9987_st2 7.30 100 5.74 5.29 5.35 5.135.33 4.81 6.05 hsa-asg-2381 hsa-asg-2381_st2 7.86 100 6.04 6.16 5.645.85 6.15 5.63 6.58 hsa-asg-3711 hsa-asg-3711_st2 7.17 100 4.43 5.775.58 6.02 5.24 5.06 5.70 hsa-cand660 hsa-cand660_st1 4.52 0 2.90 2.313.38 3.09 2.78 2.51 2.81 hsa-asg-5998 hsa-asg-5998_st1 8.01 100 6.285.83 6.86 6.29 5.83 6.10 7.41 hsa-asg-4460 hsa-asg-4460_st2 6.56 1004.58 5.18 5.61 5.35 4.97 5.06 4.60 hsa-asg-9723 hsa-asg-9723_st2 4.35 03.01 3.29 2.36 2.52 3.15 2.98 2.81 hsa-asg-6457 hsa-asg-6457_st2 6.46100 4.20 4.63 5.64 5.05 5.36 5.37 4.84 hsa-asg-2261 hsa-asg-2261_st14.70 0 3.11 3.40 3.08 3.14 3.09 3.19 4.00 hsa-asg-8428 hsa-asg-8428_st24.35 0 3.01 3.06 2.76 3.27 3.68 2.28 2.72 hsa-asg-11380hsa-asg-11380_st2 4.47 0 2.80 2.69 3.59 3.09 3.37 3.30 2.81 hsa-asg-7423hsa-asg-7423_st2 7.08 100 4.92 5.49 6.07 5.82 5.79 5.47 6.52hsa-asg-1616 hsa-asg-1616_st2 8.94 100 8.00 7.07 7.79 7.12 7.46 7.807.89 hsa-asg-3341 hsa-asg-3341_st1 5.81 100 4.53 4.93 4.49 4.24 5.144.66 4.32 hsa-asg-12666 hsa-asg-12666_st2 6.55 100 5.03 5.40 6.03 5.295.16 4.98 5.69 hsa-asg-7471 hsa-asg-7471_st1 7.19 100 6.37 6.04 5.696.01 6.01 5.90 6.17 hsa-asg-4401 hsa-asg-4401_st1 7.20 100 6.06 5.806.17 6.38 6.75 5.63 6.20 hsa-asg-2500 hsa-asg-2500_st1 6.81 100 5.525.83 5.94 6.02 6.21 5.47 5.40 hsa-asg-1124 hsa-asg-1124_st2 5.38 1004.20 4.68 4.35 4.00 4.68 4.51 4.08 hsa-asg-13798 hsa-asg-13798_st1 5.92100 4.53 5.14 4.88 5.00 5.40 4.94 4.60 hsa-asg-3798 hsa-asg-3798_st16.41 100 5.17 5.64 5.64 5.65 5.73 4.98 5.26 hsa-asg-13997hsa-asg-13997_st2 3.79 0 3.01 2.57 3.49 2.81 2.78 2.63 2.47 hsa-asg-4477hsa-asg-4477_st2 5.78 100 4.58 5.40 4.67 4.71 4.68 4.71 5.05hsa-asg-14186 hsa-asg-14186_st2 5.85 100 4.80 4.74 4.83 4.93 5.14 5.555.05 hsa-asg-11579 hsa-asg-11579_st2 6.40 100 5.10 5.73 5.76 5.91 5.785.44 5.26 hsa-asg-12271 hsa-asg-12271_st1 5.31 0 4.48 4.51 4.49 4.324.17 4.90 4.97 hsa-asg-6616 hsa-asg-6616_st1 6.83 100 5.93 6.59 5.985.82 6.23 5.96 5.99 hsa-asg-1301 hsa-asg-1301_st1 5.25 100 4.71 4.884.42 4.49 4.51 4.51 4.08 hsa-asg-841 hsa-asg-841_st1 5.59 0 4.88 4.845.05 4.92 4.95 4.51 4.95 hsa-asg-4033 hsa-asg-4033_st2 6.85 100 6.496.30 6.19 6.06 6.00 5.85 6.14 hsa-asg-4030 hsa-asg-4030_st2 5.18 0 4.534.33 4.42 4.39 5.01 4.61 4.40 hsa-asg-13924 hsa-asg-13924_st1 5.66 05.30 5.06 4.72 5.18 5.18 4.94 4.81 hsa-asg-2093 hsa-asg-2093_st1 6.37100 5.98 5.64 5.80 5.77 5.99 5.71 5.62 hsa-asg-6838 hsa-asg-6838_st16.57 0 6.00 6.25 6.27 5.97 6.05 5.96 6.33 hsa-asg-6951 hsa-asg-6951_st27.76 100 7.27 7.36 7.25 7.31 7.59 7.37 7.42 hsa-asg-8882hsa-asg-8882_st1 7.23 100 6.68 6.97 7.02 6.84 6.79 6.76 6.83hsa-asg-7271 hsa-asg-7271_st1 5.57 0 5.36 5.10 5.21 5.26 5.20 5.21 5.22hsa-asg-13348 hsa-asg-13348_st2 6.84 100 6.37 6.50 6.65 6.63 6.61 6.626.48 hsa-asg-9386 hsa-asg-9386_st2 9.26 100 9.53 9.37 9.52 9.63 9.609.58 9.74 hsa-asg-504 hsa-asg-504_st2 8.49 100 8.67 8.78 8.93 9.05 8.878.78 8.93 hsa-asg-9185 hsa-asg-9185_st2 7.89 100 8.29 8.33 8.28 8.298.08 8.21 8.36 hsa-asg-10207 hsa-asg-10207_st2 6.02 0 6.49 6.49 6.496.17 6.31 6.49 6.40 hsa-cand109 hsa-cand109_st1 6.79 100 7.04 7.23 7.207.26 7.12 7.35 7.13 hsa-cand428 hsa-cand428_st2 6.06 0 6.75 6.58 6.766.68 6.41 6.40 6.34 hsa-asg-8391 hsa-asg-8391_st1 5.07 0 5.52 5.70 5.735.43 5.59 5.28 5.81 hsa-asg-4596 hsa-asg-4596_st2 5.98 0 6.46 6.65 6.306.32 6.34 6.68 6.72 hsa-asg-797 hsa-asg-797_st2 4.80 0 5.33 5.36 5.135.43 5.38 5.14 5.48 hsa-asg-1236 hsa-asg-1236_st1 5.50 0 6.02 6.12 5.995.82 6.17 5.88 6.15 hsa-asg-6371 hsa-asg-6371_st2 8.39 100 8.78 8.848.81 9.39 9.00 8.87 8.93 hsa-asg-8322 hsa-asg-8322_st2 2.22 0 2.90 2.692.76 2.91 2.66 2.86 2.64 hsa-asg-9271 hsa-asg-9271_st2 7.35 100 7.997.90 7.51 8.07 7.91 8.04 8.13 hsa-asg-3830 hsa-asg-3830_st2 4.23 0 5.074.88 4.67 4.97 4.78 5.21 4.64 hsa-asg-4814 hsa-asg-4814_st1 5.63 0 6.086.20 6.36 6.50 6.67 5.94 6.46 hsa-asg-826 hsa-asg-826_st1 7.70 100 7.998.31 8.57 8.61 8.48 8.25 8.51 hsa-asg-9883 hsa-asg-9883_st1 6.06 0 6.466.66 7.13 6.75 7.04 6.63 6.84 hsa-cand523 hsa-cand523_st1 5.38 0 5.746.06 6.03 6.46 6.15 6.12 6.18 hsa-asg-8641 hsa-asg-8641_st1 4.29 0 4.804.88 5.15 5.29 5.14 4.71 5.33 hsa-asg-11155 hsa-asg-11155_st1 7.36 08.36 7.91 7.99 8.41 8.05 7.85 8.36 hsa-asg-12885 hsa-asg-12885_st1 4.860 5.67 5.90 5.76 5.65 5.46 5.52 5.53 hsa-asg-11521 hsa-asg-11521_st16.98 100 7.83 7.73 7.43 8.00 8.25 7.58 7.74 hsa-asg-4200hsa-asg-4200_st1 4.20 0 4.88 4.79 5.21 5.08 5.45 4.66 5.07 hsa-asg-9257hsa-asg-9257_st2 4.75 0 5.27 5.56 5.85 5.08 5.77 5.66 5.90 hsa-asg-795hsa-asg-795_st2 4.93 0 5.85 6.20 5.93 5.45 5.82 5.63 5.50 hsa-asg-5712hsa-asg-5712_st2 4.73 0 5.14 5.40 5.91 5.40 5.70 5.90 5.54 hsa-asg-4691hsa-asg-4691_st1 5.18 0 6.15 6.38 5.70 5.86 5.94 6.05 6.15 hsa-asg-5061hsa-asg-5061_st1 6.16 0 6.78 7.18 7.10 6.93 7.18 6.82 7.21 hsa-asg-1689hsa-asg-1689_st2 5.14 0 5.89 5.83 5.93 6.19 6.11 6.37 6.57 hsa-cand190hsa-cand190_st1 4.02 0 4.62 5.22 5.31 5.36 4.51 5.02 5.02 hsa-asg-13881hsa-asg-13881_st1 4.41 0 4.84 5.70 5.64 5.21 5.59 5.55 5.33 hsa-asg-9852hsa-asg-9852_st1 4.95 0 5.55 6.43 6.33 5.99 6.19 6.05 5.82 hsa-asg-4557hsa-asg-4557_st2 6.46 100 7.15 7.23 7.47 7.60 8.20 7.32 7.98hsa-asg-13230 hsa-asg-13230_st2 5.71 0 7.14 7.01 6.73 6.78 6.78 6.676.76 hsa-asg-6247 hsa-asg-6247_st1 8.70 100 9.73 9.90 10.1 9.91 9.249.80 102 hsa-asg-12460 hsa-asg-12460_st2 6.68 100 7.45 8.42 8.20 7.418.16 7.48 7.69 hsa-asg-13693 hsa-asg-13693_st2 4.82 0 5.80 6.12 6.295.92 5.95 6.08 6.24 hsa-miR157 hsa-miR157_st2 7.67 100 8.88 8.62 8.898.88 8.24 9.27 9.72 hsa-asg-3360 hsa-asg-3360_st2 5.63 0 7.37 6.62 6.686.53 6.61 7.48 7.28 hsa-asg-7009 hsa-asg-7009_st1 4.86 0 5.71 6.22 7.155.77 6.28 5.90 6.18 hsa-asg-11310 hsa-asg-11310_st2 4.95 0 6.06 6.486.91 5.58 6.30 6.32 6.18 hsa-asg-6949 hsa-asg-6949_st1 4.73 0 5.85 6.086.31 5.55 6.37 5.92 6.27 hsa-asg-6717 hsa-asg-6717_st2 5.61 0 6.20 7.257.51 6.52 7.25 6.72 7.24 hsa-asg-562 hsa-asg-562_st1 8.69 100 10.1 9.5510.2 10.1 9.74 10.3 10.7 hsa-asg-13248 hsa-asg-13248_st1 7.68 100 9.948.87 8.61 9.18 8.89 8.86 10.0 hsa-asg-13260 hsa-asg-13260_st1 6.77 1008.64 8.29 8.56 7.70 8.18 8.07 8.66 hsa-asg-6879 hsa-asg-6879_st1 4.20 05.60 5.75 5.95 5.48 5.71 5.49 6.18 hsa-asg-10883 hsa-asg-10883_st1 5.110 6.32 7.51 7.23 6.34 7.03 7.09 7.35 hsa-asg-10861 hsa-asg-10861_st23.95 0 5.62 6.28 6.43 5.59 6.32 5.55 5.65 hsa-asg-13937hsa-asg-13937_st1 6.80 100 8.27 9.59 9.28 8.05 8.75 8.88 9.64hsa-asg-7532 hsa-asg-7532_st2 3.49 0 4.62 5.64 6.39 4.84 6.51 6.05 5.81hsa-asg-13304 hsa-asg-13304_st2 4.29 0 6.15 6.40 7.12 6.29 6.82 6.287.15 hsa-asg-7074 hsa-asg-7074_st1 7.89 100 9.87 10.3 10.5 9.80 10.310.3 10.7 hsa-asg-13279 hsa-asg-13279_st1 4.77 0 8.09 7.42 7.28 7.367.25 6.90 7.66 hsa-asg-13237 hsa-asg-13237_st1 3.75 0 7.44 6.96 7.266.50 7.57 6.95 7.53 hsa-asg-870 hsa-asg-870_st1 1.58 0 4.37 6.85 7.495.18 6.42 6.05 6.50 hsa-miR-29b hsa-asg-14229_st2 9.02 100 11.3 11.010.7 10.9 10.6 10.2 11.1 hsa-miR-421 hsa-asg-5033_st2 9.32 100 7.09 6.486.07 7.53 6.19 6.64 7.66 hsa-miR-592 hsa-asg-4400_st1 6.78 100 3.20 2.692.75 3.35 3.26 2.86 3.42 LNneg LNpos1A vs LNneg MicroRNA Mean SD % ttestLog2Diff FC hsa-miR-211 4.07 1.18 0.0 1.36E−03 7.09 136 hsa-miR-375 4.270.68 0.0 1.04E−04 6.52 91 hsa-miR-203 5.84 0.71 28. 2.99E−04 5.64 50hsa-miR-200b 6.95 0.67 100 2.42E−04 5.54 46 hsa-miR-200c 7.80 0.79 1007.19E−04 5.38 41 hsa-miR-509 6.44 0.84 85. 1.21E−03 5.18 36 hsa-miR-5135.35 1.22 14. 9.74E−03 4.85 28 hsa-miR-429 2.98 0.33 0.0 1.52E−05 4.4922 hsa-miR-767-5p 2.97 0.55 0.0 2.71E−04 4.47 22 hsa-miR-187 3.68 0.620.0 8.09E−04 4.13 17 hsa-miR-592 2.99 0.47 0.0 2.41E−04 3.90 15hsa-miR-204 5.87 1.06 71. 1.36E−02 3.90 14 hsa-miR-105 3.55 0.58 0.01.73E−03 3.31 9.9 hsa-miR-132 5.56 1.13 0.0 3.50E−02 3.28 9.7hsa-miR-200a 5.49 0.58 57. 1.91E−03 3.26 9.6 hsa-miR-605 3.08 0.38 0.02.19E−04 3.24 9.4 hsa-miR-215 6.97 0.57 100 2.59E−03 3.01 8.0hsa-miR-183 6.31 0.51 85. 1.64E−03 2.95 7.7 hsa-miR-196b 7.18 0.94 1002.72E−02 2.91 7.5 hsa-miR-508 6.61 0.47 100 1.20E−03 2.89 7.4hsa-miR-493-5p 4.29 0.91 28 2.79E−02 2.81 7.0 hsa-miR-194 9.59 0.64 1007.59E−03 2.69 6.4 hsa-miR-421 7.45 0.76 100 1.75E−02 2.63 6.2hsa-miR-192 10.59 0.61 100 8.46E−03 2.53 5.8 hsa-miR-1 9.58 0.84 1004.74E−02 2.24 4.7 hsa-miR-506 6.48 0.41 100 2.37E−03 2.22 4.7hsa-miR-584 5.47 0.42 14 2.81E−03 2.20 4.6 hsa-miR-522 4.33 0.64 0.01.86E−02 2.20 4.6 hsa-miR-510 5.03 0.60 14 1.58E−02 2.12 4.3 hsa-miR-5762.87 0.39 0.0 2.68E−03 2.04 4.1 hsa-miR-495 4.88 0.47 28 9.44E−03 1.893.7 hsa-miR-182 8.62 0.49 100 1.58E−02 1.75 3.4 hsa-miR-514 2.99 0.070.0 5.89E−07 1.59 3.0 hsa-miR-450 3.85 0.58 0.0 4.45E−02 1.56 2.9hsa-miR-382 6.52 0.48 57 3.56E−02 1.38 2.6 hsa-miR-10a 11.10 0.37 1004.28E−02 1.02 2.0 hsa-miR-30d 12.31 0.27 100 2.87E−02 0.82 1.8hsa-miR-30a-5p 11.71 0.25 100 2.63E−02 0.79 1.7 hsa-miR-27b 12.15 0.12100 2.21E−03 0.66 1.6 hsa-miR-23b 13.24 0.15 100 1.71E−02 0.51 1.4hsa-miR-21 13.30 0.17 100 3.92E−02 0.47 1.4 hsa-let-7e 12.20 0.14 1002.64E−02 0.44 1.4 hsa-miR-155 13.72 0.14 100 1.54E−02 −0.52 1.4hsa-miR-195 12.86 0.15 100 4.25E−03 −0.71 1.6 hsa-miR-564 6.90 0.35 1002.89E−02 −1.07 2.1 hsa-miR-34a 8.10 0.31 100 1.59E−02 −1.11 2.2hsa-miR-22 11.56 0.47 100 4.77E−02 −1.24 2.4 hsa-miR-15a 11.71 0.35 1001.46E−02 −1.25 2.4 hsa-miR-342 13.55 0.19 100 6.27E−04 −1.31 2.5hsa-miR-186 7.78 0.50 100 3.42E−02 −1.45 2.7 hsa-miR-142-3p 5.20 0.44 147.51E−03 −1.86 3.6 hsa-miR-486 7.99 0.49 100 1.10E−02 −1.89 3.7hsa-miR-29b 10.85 0.34 100 1.86E−03 −1.90 3.7 hsa-miR-29c 10.42 0.51 1001.24E−02 −1.92 3.8 hsa-miR-199a 10.17 0.33 100 1.49E−03 −1.96 3.9hsa-miR-497 7.61 0.28 57 5.08E−04 −2.06 4.2 hsa-miR-598 7.11 0.70 852.84E−02 −2.16 4.5 hsa-asg-5638 3.56 0.77 0.0 1.68E−03 4.43 21hsa-asg-1296 3.01 0.22 0.0 6.35E−06 3.40 10 hsa-asg-7637 3.87 0.31 0.05.29E−05 3.38 10 hsa-asg-4803 4.34 0.86 0.0 1.45E−02 3.14 8.8hsa-asg-11338 3.96 0.36 0.0 3.02E−04 2.83 7.1 hsa-asg-1519 6.48 0.67 1008.73E−03 2.74 6.7 hsa-asg-14208 4.38 0.67 0.0 9.15E−03 2.72 6.6hsa-asg-9514 3.05 0.33 0.0 7.63E−04 2.20 4.6 hsa-asg-247 5.35 0.24 0.01.46E−04 2.19 4.6 hsa-asg-11361 3.36 0.28 0.0 4.74E−04 2.08 4.2hsa-asg-1268 4.22 0.59 0.0 1.96E−02 1.98 4.0 hsa-asg-9980 4.28 0.41 0.04.02E−03 1.96 3.9 hsa-asg-9987 5.39 0.40 28 4.49E−03 1.91 3.8hsa-asg-2381 6.01 0.33 85 1.97E−03 1.86 3.6 hsa-asg-3711 5.40 0.54 0.02.16E−02 1.77 3.4 hsa-cand660 2.83 0.35 0.0 4.09E−03 1.70 3.2hsa-asg-5998 6.37 0.58 71 3.70E−02 1.64 3.1 hsa-asg-4460 5.05 0.38 0.09.70E−03 1.51 2.8 hsa-asg-9723 2.87 0.34 0.0 6.18E−03 1.48 2.8hsa-asg-6457 5.01 0.50 0.0 3.53E−02 1.44 2.7 hsa-asg-2261 3.29 0.33 0.07.40E−03 1.42 2.7 hsa-asg-8428 2.97 0.44 0.0 2.66E−02 1.39 2.6hsa-asg-11380 3.09 0.34 0.0 9.30E−03 1.38 2.6 hsa-asg-7423 5.73 0.51 28.4.58E−02 1.36 2.6 hsa-asg-1616 7.59 0.38 100 1.54E−02 1.35 2.5hsa-asg-3341 4.61 0.32 0.0 1.38E−02 1.19 2.3 hsa-asg-12666 5.37 0.38 282.60E−02 1.18 2.3 hsa-asg-7471 6.03 0.21 71 2.11E−03 1.16 2.2hsa-asg-4401 6.14 0.37 100 3.60E−02 1.06 2.1 hsa-asg-2500 5.77 0.31 142.01E−02 1.04 2.1 hsa-asg-1124 4.36 0.28 0.0 1.35E−02 1.02 2.0hsa-asg-13798 4.93 0.30 0.0 2.11E−02 0.99 2.0 hsa-asg-3798 5.44 0.30 0.02.19E−02 0.97 2.0 hsa-asg-13997 2.82 0.34 0.0 3.81E−02 0.97 2.0hsa-asg-4477 4.83 0.29 0.0 2.22E−02 0.96 1.9 hsa-asg-14186 5.01 0.28 282.97E−02 0.85 1.8 hsa-asg-11579 5.57 0.30 85 4.34E−02 0.83 1.8hsa-asg-12271 4.55 0.29 0.0 4.74E−02 0.77 1.7 hsa-asg-6616 6.07 0.26 853.45E−02 0.76 1.7 hsa-asg-1301 4.51 0.25 0.0 3.35E−02 0.73 1.7hsa-asg-841 4.87 0.17 0.0 7.60E−03 0.73 1.7 hsa-asg-4033 6.15 0.21 571.97E−02 0.70 1.6 hsa-asg-4030 4.53 0.23 14 4.10E−02 0.65 1.6hsa-asg-13924 5.03 0.21 0.0 3.03E−02 0.63 1.6 hsa-asg-2093 5.79 0.15 571.07E−02 0.58 1.5 hsa-asg-6838 6.12 0.16 0.0 3.54E−02 0.45 1.4hsa-asg-6951 7.37 0.12 100 1.97E−02 0.39 1.3 hsa-asg-8882 6.84 0.12 1002.17E−02 0.39 1.3 hsa-asg-7271 5.22 0.08 14 5.51E−03 0.34 1.3hsa-asg-13348 6.55 0.11 100 4.45E−02 0.29 1.2 hsa-asg-9386 9.57 0.11 1004.82E−02 −0.30 1.2 hsa-asg-504 8.86 0.13 100 3.38E−02 −0.37 1.3hsa-asg-9185 8.26 0.09 100 9.53E−03 −0.38 1.3 hsa-asg-10207 6.41 0.12 282.90E−02 −0.38 1.3 hsa-cand109 7.19 0.10 100 1.07E−02 −0.40 1.3hsa-cand428 6.56 0.18 42 3.94E−02 −0.50 1.4 hsa-asg-8391 5.58 0.19 144.29E−02 −0.51 1.4 hsa-asg-4596 6.50 0.19 85 4.19E−02 −0.51 1.4hsa-asg-797 5.32 0.14 14 1.13E−02 −0.52 1.4 hsa-asg-1236 6.02 0.13 421.08E−02 −0.52 1.4 hsa-asg-6371 8.95 0.21 100 4.73E−02 −0.55 1.5hsa-asg-8322 2.77 0.12 0.0 4.27E−03 −0.56 1.5 hsa-asg-9271 7.94 0.20 1003.54E−02 −0.59 1.5 hsa-asg-3830 4.89 0.21 28 2.58E−02 −0.66 1.6hsa-asg-4814 6.31 0.25 71 4.69E−02 −0.68 1.6 hsa-asg-826 8.39 0.22 1002.61E−02 −0.69 1.6 hsa-asg-9883 6.79 0.23 57 2.79E−02 −0.72 1.6hsa-cand523 6.11 0.22 42 1.97E−02 −0.73 1.7 hsa-asg-8641 5.04 0.24 0.02.81E−02 −0.75 1.7 hsa-asg-11155 8.13 0.24 100 2.32E−02 −0.77 1.7hsa-asg-12885 5.64 0.15 57 3.22E−03 −0.78 1.7 hsa-asg-11521 7.80 0.27100 3.08E−02 −0.82 1.8 hsa-asg-4200 5.02 0.27 0.0 2.73E−02 −0.82 1.8hsa-asg-9257 5.58 0.31 28 4.38E−02 −0.83 1.8 hsa-asg-795 5.77 0.26 572.44E−02 −0.84 1.8 hsa-asg-5712 5.57 0.28 0.0 3.25E−02 −0.84 1.8hsa-asg-4691 6.03 0.22 0.0 1.10E−02 −0.85 1.8 hsa-asg-5061 7.03 0.18 1004.21E−03 −0.87 1.8 hsa-asg-1689 6.12 0.27 28 1.49E−02 −0.98 2.0hsa-cand190 5.01 0.33 14 3.20E−02 −0.99 2.0 hsa-asg-13881 5.41 0.31 0.02.24E−02 −1.00 2.0 hsa-asg-9852 6.05 0.30 57 1.46E−02 −1.10 2.1hsa-asg-4557 7.57 0.40 100 3.95E−02 −1.11 2.2 hsa-asg-13230 6.84 0.17100 8.05E−04 −1.13 2.2 hsa-asg-6247 9.85 0.32 100 1.51E−02 −1.15 2.2hsa-asg-12460 7.83 0.42 100 4.25E−02 −1.15 2.2 hsa-asg-13693 6.06 0.1842 6.03E−04 −1.24 2.4 hsa-miR157 8.93 0.47 100 4.56E−02 −1.26 2.4hsa-asg-3360 6.94 0.42 100 2.62E−02 −1.30 2.5 hsa-asg-7009 6.17 0.49 284.56E−02 −1.31 2.5 hsa-asg-11310 6.26 0.40 100 2.30E−02 −1.31 2.5hsa-asg-6949 6.05 0.30 28 5.91E−03 −1.32 2.5 hsa-asg-6717 6.96 0.48 713.92E−02 −1.35 2.5 hsa-asg-562 10.13 0.39 100 1.41E−02 −1.44 2.7hsa-asg-13248 9.20 0.58 100 4.88E−02 −1.52 2.9 hsa-asg-13260 8.30 0.35100 6.54E−03 −1.53 2.9 hsa-asg-6879 5.74 0.26 42. 1.35E−03 −1.54 2.9hsa-asg-10883 6.98 0.47 100 9.81E−03 −1.88 3.7 hsa-asg-10861 5.92 0.4014 3.53E−03 −1.97 3.9 hsa-asg-13937 8.92 0.62 100 1.86E−02 −2.12 4.4hsa-asg-7532 5.70 0.73 57 2.93E−02 −2.21 4.6 hsa-asg-13304 6.60 0.42 852.16E−03 −2.31 5.0 hsa-asg-7074 10.27 0.34 100 5.77E−04 −2.38 5.2hsa-asg-13279 7.42 0.37 100 5.49E−04 −2.65 6.3 hsa-asg-13237 7.17 0.39100 1.78E−04 −3.42 10 hsa-asg-870 6.12 1.05 42 6.67E−03 −4.54 23hsa-miR-29b 10.87 0.36 100 2.79E−03 −1.85 3.6 hsa-miR-421 6.81 0.63 859.87E−03 2.51 5.7 hsa-miR-592 3.07 0.30 0.0 2.50E−05 3.71 13 SD,standard deviation; %, percentage of samples expressing miRNA abovebackground levels; Log2Diff, difference in VSN-transformed expressionbetween LNpos1A and LNneg samples.

A comparison of miRNA expression between LNneg and LNposB (LNpos2B andLN pos3B) samples is shown in Table 12. Thirty six human miRNAs (13verified and 23 predicted) were determined to have significantly higheraverage expression levels in LNposB samples than in LNneg (log2Diff(LNposB vs LNneg)≧1, t-test(LNposB vs LNneg)<0.05). Among theverified miRNAs, three (hsa-miR-211, -513, and -509) were expressed atlevels between 25- and 52-fold higher in LNposB than in LNneg, and fourmiRNAs (hsa-miR-584, -204, -132, and -506) were expressed at levelsbetween 5- and 8-fold higher in LNposB samples. In addition, 85 miRNAswere expressed at levels at least 2-fold lower in LNposB samples. Amongthese, five miRNAs (hsa-miR-31, -1, -335, -85, and -192) had averageexpression levels between 15- and 25-fold lower in LNposB samples thanin LNneg samples (Table 12). Among the 23 differentially expressedpredicted miRNAs, hsa-asg-5638_st2 was expressed at levels at least18-fold higher in LNposB than in LNneg, and four miRNAs(hsa-asg-5630_st2, -11338_st2, -9186_st1, and -1946_st1) were expressedat levels between 5- and 8-fold higher in LNposB than in LNneg samples(Table 12).

TABLE 12 MicroRNAs Differentially Expressed Between Cancer-PositiveLymph Node Group B Samples (LNpos2B, -3B, and -4B) and Cancer-NegativeLymph Node Samples (LNneg1-7) from Melanoma Patients. Probe LNposB LNnegMicroRNA ID 2 3 Mean SD % 1 2 3 4 5 6 7 hsa-miR-211 hsa-miR-211_st110.07 9.48 9.77 0.41 100 3.2 5.8 2.6 4.6 5.2 4.0 3.0 hsa-miR-513hsa-miR-513_st1 10.97 9.54 10.25 1.02 100 3.0 7.1 5.5 5.2 5.4 5.5 5.8hsa-miR-509 hsa-miR-509_st2 11.96 10.54 11.25 1.01 100 6.1 8.0 6.0 5.57.0 6.3 6.2 hsa-miR-584 hsa-miR-584_st2 8.42 8.37 8.40 0.04 100 4.8 5.55.4 5.1 5.7 5.6 6.1 hsa-miR-204 hsa-miR-204_st2 7.20 9.89 8.55 1.90 1003.8 6.0 5.3 7.0 6.4 6.1 6.4 hsa-miR-132 hsa-miR-132_st1 8.48 7.63 8.060.61 50 6.6 4.4 4.5 7.4 5.4 4.8 5.9 hsa-miR-506 hsa-miR-506_st2 9.538.41 8.97 0.79 100 6.1 7.1 6.6 6.0 6.8 6.7 6.1 hsa-miR-508hsa-miR-508_st1 9.19 8.30 8.75 0.62 100 6.1 7.3 7.1 6.5 6.7 6.5 6.2hsa-miR-767-5p hsa-asg-558_st1 5.75 4.42 5.09 0.94 0 2.7 3.7 2.8 2.8 2.72.4 3.8 hsa-miR-105 hsa-miR-105_st1 6.09 4.57 5.33 1.08 0 3.0 3.3 3.63.6 2.8 4.3 4.3 hsa-miR-450 hsa-miR-450_st2 4.52 5.79 5.16 0.90 50 3.34.4 3.0 4.5 3.9 3.6 4.3 hsa-miR-514 hsa-miR-514_st1 4.40 3.98 4.19 0.300 3.0 3.1 2.9 2.9 3.0 3.0 3.0 hsa-miR-503 hsa-miR-503_st1 7.57 7.87 7.720.21 100 5.5 6.4 6.4 7.1 6.4 6.8 7.2 hsa-miR-542-3p hsa-miR-542-3p_st16.48 6.69 6.59 0.15 100 5.4 5.8 6.0 6.1 5.6 5.6 6.2 hsa-miR-638hsa-miR-638_st2 12.81 13.14 12.97 0.24 100 12.7 12.7 12.7 12.8 12.8 12.712.7 hsa-miR-26b hsa-miR-26b_st1 11.91 12.00 11.96 0.06 100 12.4 12.712.6 12.3 12.5 12.1 12.4 hsa-miR-221 hsa-miR-221_st1 12.46 11.19 11.820.90 100 12.8 12.6 12.7 12.4 12.4 12.5 12.9 hsa-let-7i hsa-let-7i_st112.60 12.49 12.54 0.08 100 13.6 13.5 13.8 13.3 13.5 13.3 13.5 hsa-let-7bhsa-let-7b_st2 12.79 12.69 12.74 0.07 100 13.5 13.7 13.9 13.6 14.1 13.513.5 hsa-miR-125b hsa-miR-125b_st1 11.22 12.43 11.83 0.86 100 12.7 12.812.8 12.8 13.2 12.5 12.7 hsa-miR-20a hsa-miR-20a_st2 10.66 11.58 11.120.65 100 12.5 12.1 11.7 12.0 11.6 12.4 12.7 hsa-miR-93 hsa-miR-93_st210.10 9.63 9.86 0.33 100 11.0 10.7 10.7 10.5 10.5 11.4 11.6 hsa-miR-141hsa-miR-141_st1 3.78 3.98 3.88 0.14 0 5.1 4.8 4.2 5.2 5.0 4.5 5.6hsa-miR-128b hsa-miR-128b_st1 9.53 9.86 9.70 0.23 100 11.3 10.6 10.410.7 10.6 10.9 11.3 hsa-miR-652 hsa-miR-652_st1 8.15 7.62 7.88 0.37 508.2 9.1 9.0 8.6 9.1 9.3 9.7 hsa-miR-27a hsa-miR-27a_st2 10.16 10.3410.25 0.13 100 10.8 11.5 11.4 11.4 11.2 11.5 11.8 hsa-miR-574hsa-miR-574_st1 6.95 7.16 7.06 0.14 100 7.6 7.9 8.7 7.3 8.7 8.6 8.5hsa-miR-146b hsa-miR-146b_st1 11.40 11.61 11.50 0.15 100 11.7 12.9 12.912.6 12.9 12.7 12.8 hsa-miR-299-5p hsa-miR-299-5p_st2 3.02 3.63 3.330.43 0 3.8 4.3 3.9 4.9 5.0 4.5 4.9 hsa-miR-214 hsa-miR-214_st1 7.18 7.517.35 0.23 0 7.7 8.5 8.6 8.3 9.3 8.6 8.5 hsa-miR-128a hsa-miR-128a_st29.10 9.49 9.30 0.27 100 11.0 10.3 10.0 10.5 10.2 10.5 10.9 hsa-miR-185hsa-miR-185_st2 9.01 8.45 8.73 0.39 100 9.5 9.6 9.8 9.5 9.7 10.6 10.8hsa-miR-29a hsa-miR-29a_st2 11.92 10.80 11.36 0.79 100 12.7 12.8 13.112.3 12.6 12.3 12.6 hsa-miR-365 hsa-miR-365_st1 4.64 5.08 4.86 0.31 05.3 5.9 6.3 6.6 6.6 5.5 6.6 hsa-miR-628 hsa-miR-628_st2 6.02 5.86 5.940.11 50 7.7 6.6 6.4 7.7 6.8 7.3 7.9 hsa-miR-24 hsa-miR-24_st1 11.9711.89 11.93 0.05 100 12.7 13.3 13.2 13.1 13.4 13.4 13.4 hsa-miR-191hsa-miR-191_st2 12.13 11.97 12.05 0.11 100 13.0 13.4 13.6 13.2 13.5 13.413.4 hsa-miR-339 hsa-miR-339_st1 5.42 5.37 5.40 0.03 0 7.1 6.6 6.4 5.96.7 6.9 7.2 hsa-miR-551b hsa-miR-551b_st1 2.64 2.80 2.72 0.11 0 3.8 3.43.8 3.9 4.3 4.7 4.3 hsa-miR-30a-3p hsa-miR-30a-3p_st1 6.67 8.10 7.381.01 100 7.9 9.2 8.9 8.8 9.4 8.0 8.7 hsa-miR-103 hsa-miR-103_st1 11.6711.40 11.54 0.19 100 12.6 12.8 13.0 12.5 12.8 13.1 13.1 hsa-miR-130bhsa-miR-130b_st1 6.29 7.28 6.78 0.70 100 7.5 8.0 8.5 7.4 7.8 8.6 9.0hsa-miR-107 hsa-miR-107_st2 11.55 11.28 11.41 0.19 100 12.5 12.6 12.912.5 12.7 12.9 13.1 hsa-miR-27b hsa-miR-27b_st1 10.40 11.22 10.81 0.58100 12.0 12.1 12.1 12.2 12.3 12.2 12.3 hsa-miR-376a hsa-miR-376a_st22.38 4.13 3.26 1.24 0 4.0 4.7 4.6 4.4 4.9 5.0 5.1 hsa-miR-142-5phsa-miR-142-5p_st2 4.58 5.35 4.96 0.54 0 5.7 6.3 6.6 5.9 7.2 6.0 6.8hsa-miR-140 hsa-miR-140_st2 5.42 6.50 5.96 0.77 50 8.1 7.1 6.5 7.5 7.17.4 8.2 hsa-miR-205 hsa-miR-205_st1 2.12 3.51 2.81 0.98 0 5.0 3.7 4.74.2 3.3 5.1 4.2 hsa-miR-100 hsa-miR-100_st2 10.15 11.14 10.64 0.70 10011.5 12.2 12.1 12.4 12.8 11.8 12.2 hsa-miR-489 hsa-miR-489_st2 3.68 4.023.85 0.24 0 4.7 5.0 5.0 5.8 5.3 5.2 6.4 hsa-miR-10b hsa-miR-10b_st210.25 11.29 10.77 0.74 100 12.2 12.3 12.4 12.4 12.5 12.0 12.4hsa-miR-106b hsa-miR-106b_st2 8.78 8.72 8.75 0.05 100 10.8 10.0 9.9 9.89.4 10.7 11.3 hsa-miR-320 hsa-miR-320_st1 9.33 8.80 9.07 0.37 100 10.210.6 10.8 10.0 11.2 10.9 10.9 hsa-miR-200b hsa-miR-200b_st1 5.24 5.455.34 0.15 50 6.3 6.1 6.9 7.1 6.8 7.4 8.1 hsa-miR-19b hsa-miR-19b_st26.36 7.34 6.85 0.69 100 8.6 8.4 8.5 7.6 8.2 8.9 9.3 hsa-miR-199bhsa-miR-199b_st2 6.23 7.84 7.03 1.13 100 8.1 8.8 8.1 8.4 9.2 8.9 9.3hsa-miR-148a hsa-miR-148a_st1 9.15 10.89 10.02 1.23 100 11.9 11.8 11.412.2 11.4 11.9 11.8 hsa-miR-189 hsa-miR-189_st1 2.51 4.57 3.54 1.45 05.2 5.4 5.0 5.2 5.6 4.7 6.0 hsa-miR-155 hsa-miR-155_st1 13.60 10.3311.96 2.32 100 13.9 13.8 13.9 13.6 13.6 13.6 13.6 hsa-miR-200chsa-miR-200c_st1 6.68 5.41 6.05 0.90 50 8.9 7.3 7.0 8.7 6.9 7.9 8.0hsa-miR-203 hsa-miR-203_st2 4.58 3.58 4.08 0.71 0 4.4 5.9 5.8 5.9 5.96.6 6.4 hsa-miR-425-5p hsa-asg-14166_st2 8.77 8.96 8.86 0.13 100 10.110.5 10.4 10.3 10.4 11.1 11.5 hsa-miR-197 hsa-miR-197_st1 8.80 7.08 7.941.21 100 9.5 9.3 9.7 9.3 9.7 10.2 10.2 hsa-miR-505 hsa-miR-505_st1 7.326.79 7.05 0.38 100 8.8 8.3 8.8 9.1 8.8 8.8 9.5 hsa-miR-101hsa-miR-101_st1 5.16 5.95 5.55 0.55 50 8.4 6.9 6.7 6.9 6.5 7.6 8.5hsa-miR-126 hsa-miR-126_st2 10.47 11.17 10.82 0.49 100 12.1 12.9 12.912.7 12.9 12.4 12.7 hsa-miR-186 hsa-miR-186_st1 5.16 6.69 5.93 1.08 507.4 8.0 7.3 7.8 8.0 7.4 8.7 hsa-miR-145 hsa-miR-145_st1 10.87 11.7811.33 0.64 100 13.0 13.4 13.1 13.4 13.4 13.0 13.0 hsa-miR-30e-3phsa-miR-30e-3p_st1 6.94 6.60 6.77 0.24 100 8.2 9.0 8.9 8.9 8.9 8.1 8.9hsa-miR-497 hsa-miR43_st1 5.35 5.80 5.57 0.31 0 7.2 7.7 7.7 7.3 7.9 7.77.9 hsa-miR-15a hsa-miR-15a_st1 9.11 10.19 9.65 0.76 100 12.1 11.8 11.411.7 11.3 11.5 12.2 hsa-miR-342 hsa-miR-342_st1 12.16 10.81 11.48 0.96100 13.4 13.7 13.8 13.3 13.6 13.5 13.5 hsa-miR-142-3p hsa-miR-142-3p_st22.77 3.48 3.13 0.50 0 5.2 5.1 5.1 5.0 4.8 5.1 6.2 hsa-miR-486hsa-miR-486_st2 6.17 5.63 5.90 0.38 0 8.2 7.4 8.2 8.1 7.2 8.5 8.3hsa-miR-22 hsa-miR-22_st1 9.66 9.24 9.45 0.30 100 10.6 11.6 11.8 11.311.8 11.9 12.0 hsa-miR-199a* hsa-miR-199a-_st1 10.51 10.89 10.70 0.27100 12.6 13.0 13.1 12.7 13.1 12.7 12.8 hsa-miR-484 hsa-miR-484_st2 6.476.02 6.24 0.32 0 8.4 8.2 8.2 8.2 8.2 8.7 9.0 hsa-miR-194 hsa-miR-194_st27.23 7.54 7.39 0.21 100 8.7 9.4 9.4 9.4 9.3 10.4 10.6 hsa-miR-331hsa-miR-331_st2 6.13 6.73 6.43 0.42 50 9.4 8.4 7.9 8.4 8.3 8.6 9.5hsa-miR-223 hsa-miR-223_st1 9.03 9.24 9.14 0.15 100 10.1 11.9 11.5 11.611.7 11.2 11.7 hsa-miR-150 hsa-miR-150_st1 12.40 9.22 10.81 2.25 10013.4 13.4 13.0 13.3 13.2 12.6 13.0 hsa-miR-224 hsa-miR-224_st2 6.09 6.486.28 0.28 100 7.1 8.3 8.3 8.8 9.6 9.2 9.1 hsa-miR-625 hsa-miR-625_st17.43 6.22 6.82 0.86 100 10.4 8.5 8.9 9.0 8.4 9.5 9.6 hsa-miR-598hsa-miR-598_st2 5.45 3.81 4.63 1.16 0 8.0 7.0 6.7 6.7 6.1 7.2 8.0hsa-miR-139 hsa-miR-139_st2 7.15 6.63 6.89 0.37 100 8.9 9.2 9.2 9.7 10.39.3 9.4 hsa-miR-133a hsa-miR-133a_st1 3.87 3.27 3.57 0.42 0 5.1 6.0 6.26.6 7.2 5.9 6.3 hsa-miR-133b hsa-miR-133b_st2 1.76 3.51 2.63 1.24 0 3.75.4 4.8 6.0 6.1 5.7 5.2 hsa-miR-629 hsa-miR-629_st1 4.99 2.83 3.91 1.530 8.2 5.7 5.8 6.9 5.8 6.7 6.9 hsa-miR-99a hsa-miR-99a_st2 7.73 9.49 8.611.25 100 10.5 11.3 11.3 11.5 11.9 11.3 11.8 hsa-miR-29b hsa-miR-29b_st18.45 7.72 8.09 0.52 100 11.3 11.0 10.8 11.0 10.5 10.3 11.1 hsa-miR-195hsa-miR-195_st2 9.62 10.58 10.10 0.68 100 12.7 13.0 12.8 12.9 13.0 12.712.8 hsa-miR-452 hsa-miR-452_st1 3.15 3.48 3.31 0.24 0 4.0 5.7 6.4 5.47.1 7.2 7.1 hsa-miR-215 hsa-miR-215_st1 3.48 4.72 4.10 0.87 0 6.3 6.96.9 6.9 6.4 7.3 8.0 hsa-miR-29c hsa-miR-29c_st2 8.12 6.96 7.54 0.82 10011.2 10.4 10.5 9.8 9.8 10.3 10.9 hsa-miR-17-3p hsa-miR-17-3p_st1 3.684.13 3.91 0.32 0 6.9 7.0 6.5 6.1 6.1 7.2 7.8 hsa-miR-199ahsa-miR-199a_st2 6.71 7.81 7.26 0.78 100 9.5 10.1 10.2 10.1 10.5 10.310.5 hsa-miR-7 hsa-miR-7_st1 6.70 6.94 6.82 0.17 100 10.3 9.7 9.1 9.69.2 10.5 10.7 hsa-miR-218 hsa-miR-218_st2 5.83 5.93 5.88 0.07 50 8.1 8.68.5 9.4 9.2 9.0 9.9 hsa-miR-425-3p hsa-miR-425_st2 3.02 3.70 3.36 0.48 05.8 6.6 6.2 5.9 6.4 7.2 7.3 hsa-miR-143 hsa-miR-143_st2 9.02 10.00 9.510.69 100 12.0 12.8 12.7 12.7 13.1 12.7 12.8 hsa-miR-192 hsa-miR-192_st26.99 6.17 6.58 0.58 100 10.2 10.2 10.6 10.2 10.0 11.3 11.6 hsa-miR-95hsa-miR-95_st1 5.49 4.24 4.86 0.88 0 8.7 8.5 8.6 9.7 8.4 9.1 10.1hsa-miR-335 hsa-miR-335_st1 5.08 5.18 5.13 0.07 0 8.3 9.4 9.0 9.2 9.79.6 10.2 hsa-miR-1 hsa-miR-1_st1 5.12 5.40 5.26 0.20 50 8.3 9.6 8.7 10.710.2 9.6 10.0 hsa-miR-31 hsa-miR-31_st2 5.83 5.88 5.86 0.04 50 7.2 10.810.5 10.3 10.5 12.2 12.0 hsa-asg-5638 hsa-asg-5638_st2 8.00 7.67 7.840.24 100 3.2 5.2 3.0 3.0 3.5 3.6 3.5 hsa-asg-5630 hsa-asg-5630_st2 7.876.45 7.16 1.00 100 6.5 4.2 3.0 3.7 2.9 4.5 3.5 hsa-asg-11338hsa-asg-11338_st2 6.88 6.40 6.64 0.34 100 4.3 3.9 3.5 3.7 4.5 3.7 4.2hsa-asg-9186 hsa-asg-9186_st1 5.83 9.76 7.79 2.78 50 4.3 6.1 5.3 5.2 5.15.1 5.2 hsa-asg-1946 hsa-asg-1946_st1 7.26 3.04 5.15 2.99 50 1.9 2.9 2.52.6 3.0 3.1 3.0 hsa-asg-14190 hsa-asg-14190_st2 6.38 3.21 4.80 2.24 502.1 2.9 3.0 3.4 2.0 3.0 3.2 hsa-asg-11966 hsa-asg-11966_st1 5.61 5.255.43 0.25 0 3.3 3.4 3.3 5.2 4.1 2.2 2.8 hsa-asg-12271 hsa-asg-12271_st15.58 7.43 6.51 1.31 50 4.5 4.5 4.5 4.3 4.2 4.9 5.0 hsa-asg-381hsa-asg-381_st1 6.34 3.40 4.87 2.08 50 2.3 4.0 2.8 3.0 2.9 2.0 3.6hsa-asg-4051 hsa-asg-4051_st1 6.29 3.10 4.69 2.26 50 2.6 2.8 2.6 2.3 2.93.0 3.4 hsa-asg-8322 hsa-asg-8322_st2 6.38 2.86 4.62 2.49 50 2.9 2.7 2.82.9 2.7 2.9 2.6 hsa-asg-12810 hsa-asg-12810_st1 6.19 3.04 4.62 2.23 503.4 2.4 2.2 2.9 2.8 2.6 3.4 hsa-asg-11238 hsa-asg-11238_st2 4.46 6.075.26 1.14 50 2.3 4.0 3.4 4.4 4.1 3.6 3.6 hsa-asg-10031 hsa-asg-10031_st14.46 6.44 5.45 1.40 50 4.6 3.3 3.9 3.5 4.1 3.5 4.9 hsa-asg-9858hsa-asg-9858_st2 8.37 7.09 7.73 0.90 100 5.7 6.4 6.3 6.7 6.3 6.5 6.6hsa-asg-12381 hsa-asg-12381_st1 6.45 8.25 7.35 1.28 50 5.7 5.9 6.1 6.56.1 5.8 6.4 hsa-asg-2261 hsa-asg-2261_st1 4.95 4.06 4.50 0.63 0 3.1 3.43.1 3.1 3.1 3.2 4.0 hsa-asg-8633 hsa-asg-8633_st1 6.89 8.27 7.58 0.97100 6.5 6.3 6.5 6.6 6.1 6.0 6.7 hsa-asg-9903 hsa-asg-9903_st2 5.61 5.575.59 0.03 50 4.4 4.1 4.5 5.2 4.3 3.3 4.9 hsa-asg-13893 hsa-asg-13893_st25.88 7.69 6.79 1.28 50 5.3 5.9 5.7 5.8 5.4 5.6 5.6 hsa-asg-4780hsa-asg-4780_st1 7.44 6.26 6.85 0.83 100 5.2 6.2 5.9 5.1 6.2 5.6 5.7hsa-asg-11850 hsa-asg-11850_st1 10.27 12.03 11.15 1.25 100 9.7 10.0 10.010.3 10.4 9.5 10.4 hsa-asg-8882 hsa-asg-8882_st1 6.92 8.89 7.91 1.40 1006.7 7.0 7.0 6.8 6.8 6.8 6.8 hsa-asg-4088 hsa-asg-4088_st2 5.45 5.49 5.470.03 50 4.5 4.0 4.4 4.9 5.1 3.7 4.7 hsa-asg-12086 hsa-asg-12086_st2 6.845.28 6.06 1.11 50 4.8 5.4 5.1 5.3 5.4 5.0 4.8 hsa-asg-8600hsa-asg-8600_st2 6.15 6.10 6.13 0.04 100 5.4 5.5 5.2 4.5 5.5 4.9 5.1hsa-asg-8700 hsa-asg-8700_st1 5.78 6.43 6.11 0.46 50 4.8 5.9 5.0 5.0 5.15.2 5.1 hsa-asg-8693 hsa-asg-8693_st1 5.81 6.88 6.34 0.76 50 5.3 5.7 5.55.8 4.8 5.2 5.5 hsa-asg-9130 hsa-asg-9130_st2 7.33 8.86 8.09 1.08 1007.0 7.3 7.1 7.5 7.1 7.3 7.2 hsa-asg-12880 hsa-asg-12880_st1 8.02 9.288.65 0.90 100 7.9 7.8 7.7 8.1 8.0 7.7 7.8 hsa-cand660 hsa-cand660_st13.37 3.85 3.61 0.34 0 2.9 2.3 3.4 3.1 2.8 2.5 2.8 hsa-asg-7252hsa-asg-7252_st1 6.97 6.14 6.55 0.59 100 5.7 5.6 6.2 5.6 6.0 5.9 5.5hsa-asg-10881 hsa-asg-10881_st1 6.52 6.36 6.44 0.11 100 5.0 5.9 5.7 5.86.1 6.0 5.8 hsa-asg-3266 hsa-asg-3266_st2 7.45 7.26 7.35 0.14 100 6.86.5 6.2 7.0 7.1 6.3 6.9 hsa-asg-11906 hsa-asg-11906_st1 5.55 5.94 5.740.28 50 5.2 5.4 5.2 5.4 5.1 4.7 4.9 hsa-asg-10815 hsa-asg-10815_st1 6.296.72 6.51 0.31 50 5.4 6.0 6.3 6.1 6.0 5.6 6.0 hsa-asg-2388hsa-asg-2388_st2 5.08 5.52 5.30 0.32 50 4.7 4.9 4.9 4.9 4.8 4.1 4.6hsa-cand913 hsa-cand913_st1 12.99 13.29 13.14 0.21 100 12.8 12.7 12.612.7 12.5 12.4 12.6 hsa-cand526 hsa-cand526_st1 5.45 4.89 5.17 0.40 504.7 4.6 4.4 4.8 4.9 4.6 4.7 hsa-asg-4217 hsa-asg-4217_st2 6.75 7.13 6.940.27 100 6.1 6.6 6.7 6.2 6.4 6.4 6.7 hsa-asg-12775 hsa-asg-12775_st18.08 7.77 7.93 0.22 100 7.4 8.0 7.3 7.5 7.3 7.2 7.3 hsa-asg-13294hsa-asg-13294_st2 6.53 6.82 6.68 0.21 100 6.4 6.3 6.3 6.5 6.1 6.1 6.2hsa-asg-1913 hsa-asg-1913_st1 5.91 5.81 5.86 0.06 0 5.7 5.6 5.7 5.8 5.45.6 5.5 hsa-asg-2826 hsa-asg-2826_st1 6.33 6.52 6.42 0.14 50 6.7 6.7 6.76.6 6.6 6.5 6.7 hsa-asg-14025 hsa-asg-14025_st1 6.34 6.30 6.32 0.03 06.4 6.6 6.6 6.9 6.7 6.7 6.6 hsa-asg-6951 hsa-asg-6951_st2 6.79 7.11 6.950.22 50 7.3 7.4 7.3 7.3 7.6 7.4 7.4 hsa-cand950 hsa-cand950_st2 5.836.50 6.16 0.47 50 6.6 6.5 6.7 6.7 6.5 6.7 6.4 hsa-asg-5032hsa-asg-5032_st2 8.67 9.15 8.91 0.34 100 9.1 9.2 9.4 9.5 9.3 9.7 9.5hsa-asg-8672 hsa-asg-8672_st1 3.87 3.21 3.54 0.46 0 4.1 3.9 4.1 4.0 4.14.2 3.7 hsa-asg-7565 hsa-asg-7565_st2 5.31 4.55 4.93 0.54 0 5.6 5.6 5.65.3 5.5 5.2 5.3 hsa-asg-1153 hsa-asg-1153_st1 6.72 6.64 6.68 0.06 50 7.37.2 7.2 7.2 7.0 7.5 7.1 hsa-asg-7569 hsa-asg-7569_st1 4.90 4.61 4.750.20 0 5.1 5.5 5.3 5.1 5.5 4.9 5.6 hsa-asg-6638 hsa-asg-6638_st2 5.164.93 5.05 0.16 0 5.8 5.8 5.8 5.4 5.8 5.5 5.2 hsa-cand109 hsa-cand109_st16.23 7.01 6.62 0.55 50 7.0 7.2 7.2 7.3 7.1 7.4 7.1 hsa-asg-1631hsa-asg-1631_st1 4.70 4.87 4.78 0.12 0 5.2 4.9 5.5 5.5 5.3 5.4 5.8hsa-asg-13143 hsa-asg-13143_st2 12.67 12.51 12.59 0.12 100 12.9 13.413.7 12.8 13.2 13.0 13.2 hsa-asg-4114 hsa-asg-4114_st2 4.85 4.79 4.820.04 0 5.2 5.9 5.4 5.1 5.2 5.6 5.5 hsa-cand407 hsa-cand407_st1 5.12 4.444.78 0.48 0 5.4 5.2 5.3 5.7 5.5 5.1 5.2 hsa-asg-7869 hsa-asg-7869_st15.86 5.33 5.59 0.37 50 6.3 5.8 5.9 6.2 6.6 6.3 6.1 hsa-asg-795hsa-asg-795_st2 4.80 5.54 5.17 0.52 50 5.8 6.2 5.9 5.5 5.8 5.6 5.5hsa-asg-826 hsa-asg-826_st1 7.40 8.16 7.78 0.54 100 8.0 8.3 8.6 8.6 8.58.3 8.5 hsa-asg-948 hsa-asg-948_st1 5.45 5.08 5.27 0.26 0 6.0 5.6 5.95.9 6.4 5.6 5.8 hsa-asg-1236 hsa-asg-1236_st1 5.04 5.76 5.40 0.51 0 6.06.1 6.0 5.8 6.2 5.9 6.2 hsa-cand458 hsa-cand458_st2 4.75 5.33 5.04 0.410 5.6 6.0 5.8 5.4 6.0 5.6 5.3 hsa-asg-10619 hsa-asg-10619_st1 5.55 5.045.30 0.36 0 6.3 6.2 6.0 6.0 5.4 5.8 5.9 hsa-cand583 hsa-cand583_st1 4.955.63 5.29 0.49 0 6.2 5.9 5.6 6.2 6.1 5.9 5.7 hsa-asg-58 hsa-asg-58_st14.40 4.24 4.32 0.11 0 5.2 5.2 4.8 4.7 4.7 5.0 5.3 hsa-asg-9815hsa-asg-9815_st2 5.67 5.85 5.76 0.13 0 6.1 6.3 6.7 6.2 6.4 6.6 6.6hsa-asg-2615 hsa-asg-2615_st2 8.31 8.90 8.61 0.42 100 9.0 9.4 9.2 9.69.1 9.1 9.7 hsa-asg-1391 hsa-asg-1391_st1 5.98 6.95 6.46 0.69 50 7.1 7.07.3 7.4 6.8 6.8 7.6 hsa-asg-4433 hsa-asg-4433_st1 4.46 4.44 4.45 0.02 05.2 5.5 5.3 4.9 5.0 5.4 4.8 hsa-asg-1545 hsa-asg-1545_st1 4.90 5.33 5.110.30 0 5.9 6.0 5.9 5.5 6.1 5.7 5.7 hsa-asg-10575 hsa-asg-10575_st1 6.337.45 6.89 0.80 100 7.6 7.6 7.8 7.8 7.3 7.4 7.8 hsa-asg-2134hsa-asg-2134_st2 3.68 4.44 4.06 0.53 0 4.4 5.1 4.8 5.1 4.8 4.5 4.7hsa-asg-1599 hsa-asg-1599_st1 4.19 4.61 4.40 0.30 0 5.0 4.9 5.4 5.5 4.94.9 5.3 hsa-asg-555 hsa-asg-555_st1 6.15 6.82 6.49 0.47 50 6.6 7.2 7.17.3 7.2 7.5 7.6 hsa-asg-1941 hsa-asg-1941_st2 4.58 3.53 4.06 0.74 0 4.65.3 4.8 4.8 4.8 4.6 4.7 hsa-asg-2843 hsa-asg-2843_st1 4.19 4.67 4.430.34 0 5.2 5.3 4.8 5.2 5.3 5.5 5.0 hsa-asg-11867 hsa-asg-11867_st1 4.194.40 4.30 0.15 0 5.5 4.7 5.1 4.9 5.1 5.1 4.9 hsa-asg-9739hsa-asg-9739_st1 4.64 4.84 4.74 0.14 50 5.1 5.2 5.8 5.4 5.6 5.3 5.9hsa-asg-11329 hsa-asg-11329_st1 5.04 3.94 4.49 0.78 0 5.4 5.5 5.0 5.45.4 4.9 5.0 hsa-asg-4196 hsa-asg-4196_st2 5.28 5.70 5.49 0.30 0 6.1 6.66.2 6.0 6.5 6.2 6.2 hsa-asg-9597 hsa-asg-9597_st2 4.33 4.20 4.27 0.09 05.7 5.0 5.1 4.8 5.6 4.6 4.7 hsa-asg-2041 hsa-asg-2041_st1 4.75 4.73 4.740.01 0 5.3 5.1 5.7 5.7 5.3 5.7 6.0 hsa-asg-10914 hsa-asg-10914_st1 3.684.31 4.00 0.44 0 4.6 4.8 4.5 4.7 4.9 5.2 4.9 hsa-asg-8598hsa-asg-8598_st2 4.99 5.35 5.17 0.25 0 5.9 6.1 6.4 5.4 6.1 6.0 6.0hsa-asg-4587 hsa-asg-4587_st1 4.80 4.74 4.77 0.04 0 5.9 5.6 5.5 5.5 5.65.6 5.3 hsa-asg-7643 hsa-asg-7643_st2 7.43 6.99 7.21 0.31 0 7.8 7.9 7.97.4 8.0 8.5 8.7 hsa-asg-2693 hsa-asg-2693_st1 5.67 5.31 5.49 0.25 0 6.16.4 6.6 5.8 6.6 6.7 6.0 hsa-asg-6651 hsa-asg-6651_st2 5.49 5.22 5.350.19 0 6.5 5.9 6.6 6.0 6.1 6.1 6.1 hsa-asg-8675 hsa-asg-8675_st1 5.526.28 5.90 0.54 0 6.5 6.1 6.8 7.1 6.8 6.7 7.0 hsa-asg-5576hsa-asg-5576_st1 5.20 5.19 5.20 0.01 0 5.4 6.4 6.5 5.5 6.0 6.5 6.3hsa-asg-8477 hsa-asg-8477_st1 6.36 7.20 6.78 0.60 100 8.0 7.9 7.6 7.87.2 7.3 7.8 hsa-asg-6838 hsa-asg-6838_st1 5.12 5.37 5.25 0.18 0 6.0 6.36.3 6.0 6.1 6.0 6.3 hsa-asg-2989 hsa-asg-2989_st1 4.11 3.98 4.05 0.10 04.3 4.8 5.2 5.5 4.9 4.7 5.2 hsa-asg-10796 hsa-asg-10796_st1 5.52 4.705.11 0.57 0 6.1 6.2 6.2 5.8 6.1 5.8 5.8 hsa-asg-6005 hsa-asg-6005_st15.64 6.78 6.21 0.80 50 6.8 6.9 7.5 6.7 7.5 7.0 7.3 hsa-asg-9562hsa-asg-9562_st2 4.40 4.67 4.53 0.19 0 5.6 5.9 5.2 5.5 6.1 4.8 5.0hsa-asg-12049 hsa-asg-12049_st2 5.91 6.91 6.41 0.71 50 7.3 7.3 7.7 7.36.8 7.2 7.6 hsa-asg-1362 hsa-asg-1362_st2 3.87 3.53 3.70 0.24 0 4.8 4.04.2 4.6 5.4 4.7 4.4 hsa-asg-9004 hsa-asg-9004_st1 6.84 7.44 7.14 0.42100 8.0 7.8 7.8 8.4 8.0 8.5 7.9 hsa-asg-593 hsa-asg-593_st2 3.68 3.583.63 0.07 0 4.9 4.6 4.0 4.4 4.6 4.7 4.6 hsa-asg-11181 hsa-asg-11181_st14.58 5.49 5.03 0.64 0 6.0 5.8 5.1 6.1 6.3 5.9 6.5 hsa-asg-6634hsa-asg-6634_st2 3.37 4.31 3.84 0.66 0 4.8 5.1 5.2 4.7 4.9 3.8 4.9hsa-asg-3340 hsa-asg-3340_st2 4.64 5.73 5.19 0.77 0 5.6 6.2 6.6 6.3 5.85.8 6.6 hsa-asg-10506 hsa-asg-10506_st2 4.64 5.65 5.14 0.71 0 6.2 5.86.1 6.0 6.0 6.2 6.3 hsa-asg-11380 hsa-asg-11380_st2 1.43 2.86 2.15 1.010 2.8 2.7 3.6 3.1 3.4 3.3 2.8 hsa-cand902 hsa-cand902_st1 4.52 5.77 5.150.88 0 5.5 6.1 6.3 6.0 6.4 5.8 6.4 hsa-asg-12460 hsa-asg-12460_st2 6.796.95 6.87 0.11 100 7.4 8.4 8.2 7.4 8.2 7.5 7.7 hsa-asg-8271hsa-asg-8271_st2 5.16 5.59 5.38 0.30 0 6.0 6.2 6.4 6.2 6.5 6.2 7.0hsa-asg-14186 hsa-asg-14186_st2 3.37 4.64 4.01 0.89 0 4.8 4.7 4.8 4.95.1 5.6 5.1 hsa-asg-10197 hsa-asg-10197_st2 5.67 6.43 6.05 0.54 50 6.47.3 7.1 7.3 6.6 6.8 7.8 hsa-asg-4599 hsa-asg-4599_st1 4.04 5.43 4.730.99 50 5.6 5.5 5.7 6.1 5.9 5.8 5.7 hsa-asg-3231 hsa-asg-3231_st2 10.0410.79 10.42 0.53 100 11.0 11.8 11.6 11.5 11.2 11.5 11.6 hsa-asg-12996hsa-asg-12996_st1 5.52 5.98 5.75 0.33 50 6.7 6.3 6.7 7.1 6.7 6.8 7.2hsa-asg-10041 hsa-asg-10041_st1 3.68 4.40 4.04 0.51 0 5.5 5.4 4.4 4.75.0 5.3 5.3 hsa-asg-4515 hsa-asg-4515_st1 3.78 4.47 4.12 0.49 0 4.9 5.14.7 4.9 5.2 5.5 5.9 hsa-asg-7471 hsa-asg-7471_st1 4.99 4.96 4.97 0.02 06.4 6.0 5.7 6.0 6.0 5.9 6.2 hsa-asg-14189 hsa-asg-14189_st2 11.06 10.8410.95 0.15 100 11.8 12.3 12.4 11.7 11.7 12.2 12.1 hsa-asg-9530hsa-asg-9530_st2 10.78 11.83 11.31 0.74 100 12.2 12.5 12.6 12.4 12.012.3 12.6 hsa-asg-2046 hsa-asg-2046_st2 3.15 4.24 3.69 0.77 0 4.5 5.05.2 4.9 4.8 4.1 4.8 hsa-asg-5467 hsa-asg-5467_st1 4.80 3.85 4.33 0.67 05.4 5.9 5.7 5.3 5.6 4.3 5.7 hsa-asg-11096 hsa-asg-11096_st1 4.26 5.524.89 0.89 0 6.0 5.9 6.0 5.7 5.9 5.7 6.6 hsa-miR246 hsa-miR246_st2 4.265.00 4.63 0.52 0 5.9 5.6 5.3 5.6 5.8 5.2 6.6 hsa-asg-1261hsa-asg-1261_st1 3.87 5.16 4.52 0.92 0 5.1 5.8 5.6 6.0 5.4 5.8 5.6hsa-asg-10183 hsa-asg-10183_st1 3.58 4.53 4.05 0.67 0 4.7 5.1 5.3 5.35.0 5.0 5.7 hsa-asg-9729 hsa-asg-9729_st2 5.75 5.35 5.55 0.29 50 6.6 6.87.0 6.4 6.7 6.7 6.4 hsa-cand588 hsa-cand588_st1 5.55 5.49 5.52 0.04 06.9 6.2 6.9 6.5 6.2 6.5 7.4 hsa-asg-3509 hsa-asg-3509_st1 4.40 5.42 4.910.72 0 5.4 6.6 6.6 5.8 5.9 5.4 6.6 hsa-asg-6949 hsa-asg-6949_st1 4.585.23 4.91 0.46 0 5.8 6.1 6.3 5.5 6.4 5.9 6.3 hsa-asg-2353hsa-asg-2353_st1 3.26 3.90 3.58 0.45 0 5.1 4.4 4.3 4.8 5.3 4.2 5.0hsa-miR-594 hsa-miR-594_st2 7.32 7.23 7.28 0.06 0 8.2 7.3 9.5 8.5 8.58.5 8.5 hsa-asg-6761 hsa-asg-6761_st1 3.37 4.47 3.92 0.77 0 4.9 4.9 5.44.6 5.6 5.0 5.2 hsa-asg-11373 hsa-asg-11373_st1 6.92 6.59 6.75 0.23 07.2 8.4 8.5 7.4 8.2 7.9 7.8 hsa-cand190 hsa-cand190_st1 3.68 4.02 3.850.24 0 4.6 5.2 5.3 5.4 4.5 5.0 5.0 hsa-asg-9403 hsa-asg-9403_st2 6.917.88 7.39 0.68 100 7.9 8.3 9.3 8.6 8.5 8.4 8.8 hsa-asg-7275hsa-asg-7275_st2 3.37 4.89 4.13 1.07 50 5.4 5.0 5.7 5.5 5.6 5.3 4.7hsa-asg-6880 hsa-asg-6880_st1 3.87 5.56 4.71 1.20 0 6.5 5.8 5.5 5.8 5.55.9 6.3 hsa-asg-11893 hsa-asg-11893_st2 6.43 6.95 6.69 0.36 100 7.6 7.87.9 8.0 7.6 7.8 8.3 hsa-asg-271 hsa-asg-271_st1 2.77 3.77 3.27 0.70 04.2 4.5 4.3 4.5 4.6 4.2 4.9 hsa-asg-8917 hsa-asg-8917_st2 5.78 6.75 6.260.69 50 6.4 8.0 8.1 7.1 7.5 7.4 7.7 hsa-asg-2273 hsa-asg-2273_st1 3.783.94 3.86 0.11 0 4.7 4.7 5.4 5.8 6.0 4.2 4.6 hsa-asg-11310hsa-asg-11310_st2 5.20 4.91 5.06 0.20 0 6.1 6.5 6.9 5.6 6.3 6.3 6.2hsa-asg-4924 hsa-asg-4924_st2 4.26 4.06 4.16 0.14 0 5.8 4.9 5.1 5.7 5.45.0 5.7 hsa-asg-2750 hsa-asg-2750_st2 9.29 11.25 10.27 1.38 100 11.211.7 11.6 11.6 11.2 11.3 11.9 hsa-asg-7009 hsa-asg-7009_st1 4.19 5.694.94 1.06 0 5.7 6.2 7.2 5.8 6.3 5.9 6.2 hsa-asg-1305 hsa-asg-1305_st24.26 4.79 4.53 0.38 0 5.1 6.3 5.6 6.3 5.3 5.3 6.3 hsa-asg-7688hsa-asg-7688_st1 4.85 5.43 5.14 0.41 50 6.4 6.0 6.5 6.2 6.4 6.3 6.8hsa-asg-4469 hsa-asg-4469_st1 5.31 6.58 5.95 0.89 50 7.1 7.3 7.5 6.8 7.27.1 7.5 hsa-miR257 hsa-miR257_st1 7.57 8.30 7.93 0.52 100 9.7 9.0 8.29.5 8.7 9.2 9.9 hsa-asg-6457 hsa-asg-6457_st2 3.37 4.13 3.75 0.54 0 4.24.6 5.6 5.1 5.4 5.4 4.8 hsa-asg-14197_st1 hsa-asg-14197_st1 8.23 8.548.39 0.22 100 9.2 10.2 10.3 9.8 9.4 8.7 10.0 hsa-asg-12666hsa-asg-12666_st2 3.78 4.40 4.09 0.44 0 5.0 5.4 6.0 5.3 5.2 5.0 5.7hsa-asg-6357 hsa-asg-6357_st1 3.78 5.50 4.64 1.22 0 6.5 5.5 6.2 5.7 5.95.2 6.4 hsa-asg-2824 hsa-asg-2824_st2 4.70 4.50 4.60 0.14 0 6.1 6.3 6.84.8 5.8 5.7 5.7 hsa-asg-12339 hsa-asg-12339_st1 5.04 6.16 5.60 0.80 506.6 7.0 6.3 7.0 6.6 6.9 8.0 hsa-asg-12765 hsa-asg-12765_st2 3.78 4.103.94 0.22 0 4.6 4.8 4.9 5.6 6.0 5.9 4.6 hsa-asg-7319 hsa-asg-7319_st23.87 4.61 4.24 0.53 0 5.6 5.3 5.5 5.5 5.7 5.3 5.9 hsa-asg-3829hsa-asg-3829_st1 2.51 4.17 3.34 1.17 0 4.0 4.6 4.7 4.8 5.4 4.8 4.4hsa-asg-3070 hsa-asg-3070_st2 3.68 4.20 3.94 0.37 0 4.6 5.1 5.9 5.6 5.85.4 5.0 hsa-asg-2343 hsa-asg-2343_st2 5.45 3.32 4.39 1.51 0 6.1 5.6 6.15.5 6.2 5.3 5.6 hsa-asg-8018 hsa-asg-8018_st1 3.78 4.00 3.89 0.16 0 6.14.9 5.3 5.0 5.1 5.3 5.2 hsa-asg-1268 hsa-asg-1268_st1 2.51 3.10 2.810.41 0 4.1 4.0 3.2 4.5 4.1 4.8 4.9 hsa-asg-13693 hsa-asg-13693_st2 4.754.54 4.64 0.15 0 5.8 6.1 6.3 5.9 6.0 6.1 6.2 hsa-asg-7532hsa-asg-7532_st2 4.19 4.37 4.28 0.13 0 4.6 5.6 6.4 4.8 6.5 6.0 5.8hsa-asg-925 hsa-asg-925_st1 6.36 5.06 5.71 0.92 50 8.1 7.0 7.1 7.1 6.27.0 7.8 hsa-asg-3712 hsa-asg-3712_st1 4.46 6.23 5.35 1.25 0 5.7 7.4 7.56.6 7.0 6.7 6.9 hsa-asg-12346 hsa-asg-12346_st2 5.67 5.76 5.71 0.06 06.4 7.4 7.2 6.7 7.9 7.2 7.6 hsa-asg-14233 hsa-asg-14233_st1 7.55 8.377.96 0.58 100 8.8 9.8 9.1 9.4 9.9 9.4 9.9 hsa-asg-13976hsa-asg-13976_st2 4.33 4.39 4.36 0.04 0 5.6 5.8 6.4 5.7 6.0 5.8 5.7hsa-asg-14150 hsa-asg-14150_st1 5.42 5.53 5.48 0.08 0 6.0 7.1 7.0 6.57.2 7.5 7.7 hsa-asg-6717 hsa-asg-6717_st2 5.42 5.47 5.44 0.04 0 6.2 7.37.5 6.5 7.3 6.7 7.2 hsa-asg-10981 hsa-asg-10981_st1 3.48 4.17 3.83 0.490 5.2 5.5 5.8 5.7 6.0 4.8 4.3 hsa-asg-6247 hsa-asg-6247_st1 7.97 8.698.33 0.51 100 9.7 9.9 10.2 9.9 9.2 9.8 10.2 hsa-asg-7782hsa-asg-7782_st2 4.95 5.66 5.30 0.51 0 5.6 7.5 7.5 6.9 6.9 6.0 7.4hsa-asg-2365 hsa-asg-2365_st1 3.78 4.39 4.08 0.43 0 5.6 5.5 5.8 5.3 5.85.8 5.7 hsa-asg-11896 hsa-asg-11896_st2 9.72 11.92 10.82 1.56 100 12.312.6 12.7 12.4 11.9 12.2 12.7 hsa-asg-3854 hsa-asg-3854_st1 4.11 4.244.18 0.09 0 5.9 6.1 6.1 5.5 5.7 5.6 5.6 hsa-asg-9508 hsa-asg-9508_st18.74 8.94 8.84 0.14 0 9.4 10.9 11.1 9.9 10.3 10.7 10.8 hsa-asg-13646hsa-asg-13646_st1 5.91 5.77 5.84 0.10 0 7.4 7.3 6.9 7.4 7.4 7.5 8.5hsa-asg-12702 hsa-asg-12702_st1 6.56 4.77 5.67 1.27 50 7.4 7.0 8.6 6.07.1 7.5 7.6 hsa-cand345 hsa-cand345_st1 4.33 4.55 4.44 0.16 0 6.3 5.65.7 6.2 6.2 5.9 6.8 hsa-asg-6413 hsa-asg-6413_st2 4.19 3.85 4.02 0.24 05.1 5.9 6.5 4.7 6.1 5.8 5.7 hsa-asg-9852 hsa-asg-9852_st1 5.12 3.60 4.361.07 0 5.5 6.4 6.3 6.0 6.2 6.0 5.8 hsa-asg-10473 hsa-asg-10473_st1 3.954.17 4.06 0.15 0 5.8 5.9 5.7 5.8 5.3 5.8 5.9 hsa-asg-12105hsa-asg-12105_st2 3.02 4.65 3.84 1.15 0 5.4 5.9 4.5 6.2 6.2 5.6 4.9hsa-asg-2872 hsa-asg-2872_st2 8.15 7.95 8.05 0.14 100 9.3 9.9 10.5 8.910.2 9.6 9.9 hsa-asg-8617 hsa-asg-8617_st1 4.19 5.65 4.92 1.03 0 5.4 7.07.2 6.8 6.7 6.3 7.2 hsa-asg-797 hsa-asg-797_st2 3.37 3.81 3.59 0.31 05.3 5.4 5.1 5.4 5.4 5.1 5.5 hsa-asg-7149 hsa-asg-7149_st2 4.58 4.57 4.580.01 0 5.6 6.6 6.6 5.2 7.0 6.4 6.8 hsa-asg-12973 hsa-asg-12973_st2 4.264.34 4.30 0.06 0 5.6 6.1 6.5 5.8 6.1 6.1 6.4 hsa-asg-12902hsa-asg-12902_st1 5.75 4.34 5.05 1.00 0 7.7 7.3 6.6 6.7 7.0 5.9 6.6hsa-asg-13097 hsa-asg-13097_st1 3.48 2.98 3.23 0.35 0 3.4 4.2 5.3 4.85.8 5.8 6.2 hsa-asg-10861 hsa-asg-10861_st2 3.87 4.29 4.08 0.30 0 5.66.3 6.4 5.6 6.3 5.6 5.7 hsa-asg-8682 hsa-asg-8682_st2 4.40 5.16 4.780.54 50 4.9 7.4 7.6 5.8 6.9 6.7 7.3 hsa-asg-13205 hsa-asg-13205_st1 4.955.36 5.15 0.29 0 5.5 7.3 7.0 6.4 7.7 7.5 7.8 hsa-cand768 hsa-cand768_st19.07 9.92 9.49 0.60 0 11.1 11.7 11.5 11.8 10.7 10.8 12.0 hsa-asg-12759hsa-asg-12759_st2 2.64 3.81 3.23 0.83 0 4.6 5.7 5.8 3.7 5.8 4.6 5.8hsa-asg-7080 hsa-asg-7080_st1 2.00 3.63 2.81 1.15 0 4.1 4.7 5.0 4.9 5.14.4 5.0 hsa-asg-3360 hsa-asg-3360_st2 4.58 5.44 5.01 0.61 0 7.4 6.6 6.76.5 6.6 7.5 7.3 hsa-asg-10149 hsa-asg-10149_st1 5.91 5.44 5.67 0.33 07.4 7.7 8.4 6.8 7.8 7.5 7.8 hsa-asg-11612 hsa-asg-11612_st1 3.58 4.083.83 0.35 0 5.4 6.0 5.7 6.4 5.6 4.7 6.6 hsa-asg-6138 hsa-asg-6138_st13.15 3.53 3.34 0.27 0 4.5 5.9 5.6 5.1 5.1 5.5 5.6 hsa-asg-13244hsa-asg-13244_st1 5.31 4.39 4.85 0.65 0 7.7 7.0 7.1 6.6 6.9 6.2 6.4hsa-cand965 hsa-cand965_st1 5.08 5.50 5.29 0.30 0 7.2 7.9 7.7 5.9 7.67.2 7.5 hsa-asg-13230 hsa-asg-13230_st2 5.31 4.34 4.83 0.69 0 7.1 7.06.7 6.8 6.8 6.7 6.8 hsa-asg-14245 hsa-asg-14245_st1 10.60 9.49 10.040.78 100 12.9 11.3 12.1 12.1 11.6 12.1 12.3 hsa-asg-10263hsa-asg-10263_st2 2.77 4.40 3.59 1.15 0 5.7 5.3 5.0 5.9 5.3 6.4 5.9hsa-asg-21 hsa-asg-21_st2 3.87 4.10 3.98 0.16 0 5.7 5.8 6.9 4.6 6.6 6.36.4 hsa-asg-12923 hsa-asg-12923_st1 3.37 4.17 3.77 0.56 0 5.0 6.2 7.04.5 6.5 5.9 6.0 hsa-asg-4002 hsa-asg-4002_st1 4.11 5.96 5.04 1.31 50 5.87.6 7.9 6.0 7.7 7.2 7.5 hsa-asg-9298 hsa-asg-9298_st2 1.88 2.86 2.370.70 0 3.4 4.7 5.2 2.6 4.7 4.8 5.9 hsa-asg-13258 hsa-asg-13258_st2 6.025.45 5.74 0.40 0 7.8 7.7 7.7 7.6 7.5 7.8 8.8 hsa-asg-13260hsa-asg-13260_st1 6.62 5.72 6.17 0.64 50 8.6 8.3 8.6 7.7 8.2 8.1 8.7hsa-asg-14170 hsa-asg-14170_st1 3.26 5.16 4.21 1.35 0 6.5 6.7 6.1 7.46.9 5.5 5.5 hsa-asg-242 hsa-asg-242_st2 2.64 4.22 3.43 1.12 0 3.3 5.75.6 6.7 6.7 6.3 5.4 hsa-asg-870 hsa-asg-870_st1 4.40 3.30 3.85 0.78 04.4 6.8 7.5 5.2 6.4 6.0 6.5 hsa-asg-4557 hsa-asg-4557_st2 4.75 5.83 5.290.76 50 7.1 7.2 7.5 7.6 8.2 7.3 8.0 hsa-asg-5303 hsa-asg-5303_st1 3.955.42 4.69 1.04 0 6.5 8.0 6.8 7.2 7.2 6.8 6.4 hsa-asg-8208hsa-asg-8208_st1 2.64 4.90 3.77 1.60 0 5.4 6.4 6.8 4.8 6.1 5.9 7.1hsa-miR157 hsa-miR157_st2 6.98 6.24 6.61 0.52 100 8.9 8.6 8.9 8.9 8.29.3 9.7 hsa-asg-818 hsa-asg-818_st1 2.00 3.98 2.99 1.40 0 4.7 5.3 4.86.8 5.2 3.8 7.0 hsa-asg-13248 hsa-asg-13248_st1 6.94 6.52 6.73 0.30 1009.9 8.9 8.6 9.2 8.9 8.9 10.1 hsa-asg-562 hsa-asg-562_st1 8.05 7.05 7.550.70 100 10.1 9.6 10.2 10.2 9.7 10.3 10.8 hsa-asg-13937hsa-asg-13937_st1 5.35 7.30 6.33 1.38 50 8.3 9.6 9.3 8.0 8.7 8.9 9.6hsa-asg-13304 hsa-asg-13304_st2 3.26 4.62 3.94 0.96 0 6.1 6.4 7.1 6.36.8 6.3 7.2 hsa-asg-13354 hsa-asg-13354_st1 5.86 6.09 5.97 0.17 0 8.58.6 8.9 9.2 7.9 7.6 10.0 hsa-asg-5304 hsa-asg-5304_st1 7.67 7.58 7.630.06 50 11.0 10.1 9.9 10.2 10.2 10.1 10.6 hsa-miR154 hsa-miR154_st1 2.123.32 2.72 0.85 0 3.8 5.0 5.5 5.2 6.2 5.8 6.5 hsa-asg-10883hsa-asg-10883_st1 3.87 4.68 4.27 0.57 0 6.3 7.5 7.2 6.3 7.0 7.1 7.4hsa-asg-10387 hsa-asg-10387_st1 4.11 5.98 5.05 1.32 50 6.3 8.4 8.7 6.18.7 8.0 8.2 hsa-asg-10676 hsa-asg-10676_st1 3.78 3.43 3.60 0.25 0 4.86.6 5.9 7.1 5.8 6.6 8.0 hsa-asg-7074 hsa-asg-7074_st1 8.69 6.17 7.431.78 50 9.9 10.3 10.5 9.8 10.3 10.3 10.8 hsa-asg-13308 hsa-asg-13308_st27.09 7.50 7.30 0.29 0 11.0 10.1 9.6 10.1 10.0 9.9 10.6 hsa-asg-10580hsa-asg-10580_st1 4.58 4.81 4.69 0.16 0 7.6 7.5 7.7 8.3 7.1 6.0 9.0hsa-asg-14160 hsa-asg-14160_st1 2.77 2.14 2.46 0.45 0 5.6 5.5 5.0 4.06.0 6.0 5.8 hsa-asg-4205 hsa-asg-4205_st2 2.90 2.95 2.93 0.04 0 4.4 6.66.4 4.5 6.8 6.3 6.6 hsa-asg-10666 hsa-asg-10666_st1 2.51 3.96 3.24 1.020 6.2 6.8 7.1 4.6 6.8 5.8 6.4 hsa-asg-13279 hsa-asg-13279_st1 4.40 4.114.26 0.20 0 8.1 7.4 7.3 7.4 7.2 6.9 7.7 hsa-asg-14133 hsa-asg-14133_st23.26 5.71 4.49 1.73 0 8.8 6.5 6.7 8.7 6.7 7.8 8.7 hsa-asg-13237hsa-asg-13237_st1 3.48 3.63 3.55 0.10 0 7.4 7.0 7.3 6.5 7.6 7.0 7.5hsa-miR-100 hsa-cand375_st1 10.31 11.30 10.81 0.70 100 11.7 12.2 12.112.4 12.8 12.0 12.3 hsa-miR-126 hsa-cand497_st1 10.16 10.99 10.57 0.59100 12.2 12.8 12.8 12.4 12.6 12.3 12.5 hsa-miR-140 hsa-asg-14239_st15.72 6.47 6.10 0.53 50 8.4 7.9 6.6 7.7 7.1 7.6 8.6 hsa-miR-199a*hsa-asg-14140_st1 10.49 10.90 10.70 0.29 100 12.6 13.0 13.0 12.7 13.012.7 12.8 hsa-miR-224 hsa-asg-14247_st1 6.65 7.09 6.87 0.31 100 7.7 8.68.6 9.1 9.7 9.3 9.2 hsa-miR-29b hsa-asg-14229_st2 8.62 7.70 8.16 0.65100 11.3 11.0 10.7 11.0 10.6 10.3 11.2 hsa-miR-331 hsa-asg-14205_st14.52 5.29 4.90 0.54 0 7.8 7.1 6.9 7.1 7.4 7.4 8.1 hsa-miR-365hsa-cand708_st1 3.58 3.81 3.70 0.16 0 5.0 4.8 4.4 4.8 5.4 4.6 5.0hsa-miR-365 hsa-asg-13156_st1 4.26 5.18 4.72 0.65 0 4.9 6.5 6.1 6.3 6.75.0 6.5 hsa-miR-376a hsa-asg-10049_st1 3.68 3.43 3.56 0.18 0 3.6 4.4 4.44.3 5.0 5.4 5.1 hsa-miR-421 hsa-asg-5033_st2 7.27 7.77 7.52 0.35 100 7.16.5 6.1 7.5 6.2 6.6 7.7 hsa-miR-503 hsa-miR141_st1 8.15 8.02 8.09 0.09100 6.6 6.3 6.8 7.8 6.6 7.7 7.5 hsa-miR-551b hsa-miR167_st2 3.37 2.482.93 0.63 0 3.7 4.0 4.0 2.6 4.3 3.5 4.3 hsa-miR-592 hsa-asg-4400_st13.02 4.72 3.87 1.20 0 3.2 2.7 2.8 3.4 3.3 2.9 3.4 LNneg LNposB vs LNnegMicroRNA Mean SD % ttest Log2Diff FC hsa-miR-211 4.1 1.18 0.0 3.53E−045.70 52.1 hsa-miR-513 5.4 1.22 14.3 1.33E−03 4.90 29.9 hsa-miR-509 6.40.84 85.7 2.32E−04 4.81 28.0 hsa-miR-584 5.5 0.42 14.3 3.45E−05 2.92 7.6hsa-miR-204 5.9 1.06 71.4 2.85E−02 2.67 6.4 hsa-miR-132 5.6 1.13 0.02.30E−02 2.50 5.6 hsa-miR-506 6.5 0.41 100.0 3.73E−04 2.49 5.6hsa-miR-508 6.6 0.47 100.0 1.04E−03 2.13 4.4 hsa-miR-767-5p 3.0 0.55 0.03.80E−03 2.12 4.3 hsa-miR-105 3.5 0.58 0.0 1.31E−02 1.78 3.4 hsa-miR-4503.9 0.58 0.0 3.64E−02 1.31 2.5 hsa-miR-514 3.0 0.07 0.0 7.72E−06 1.202.3 hsa-miR-503 6.6 0.59 42.9 3.59E−02 1.15 2.2 hsa-miR-542-3p 5.8 0.2871.4 8.85E−03 0.77 1.7 hsa-miR-638 12.7 0.07 100.0 2.33E−02 0.26 1.2hsa-miR-26b 12.4 0.21 100.0 2.12E−02 −0.46 1.4 hsa-miR-221 12.6 0.20100.0 3.87E−02 −0.78 1.7 hsa-let-7i 13.5 0.17 100.0 1.72E−04 −0.95 1.9hsa-let-7b 13.7 0.25 100.0 1.30E−03 −0.96 1.9 hsa-miR-125b 12.8 0.20100.0 1.46E−02 −0.96 2.0 hsa-miR-20a 12.1 0.41 100.0 2.76E−02 −1.00 2.0hsa-miR-93 10.9 0.42 100.0 1.57E−02 −1.04 2.1 hsa-miR-141 4.9 0.44 14.31.61E−02 −1.05 2.1 hsa-miR-128b 10.8 0.36 100.0 5.23E−03 −1.11 2.2hsa-miR-652 9.0 0.47 100.0 1.85E−02 −1.11 2.2 hsa-miR-27a 11.4 0.29100.0 1.31E−03 −1.12 2.2 hsa-miR-574 8.2 0.57 100.0 3.16E−02 −1.14 2.2hsa-miR-146b 12.6 0.42 100.0 8.81E−03 −1.15 2.2 hsa-miR-299-5p 4.5 0.4914.3 2.15E−02 −1.15 2.2 hsa-miR-214 8.5 0.46 100.0 1.22E−02 −1.17 2.2hsa-miR-128a 10.5 0.37 100.0 4.11E−03 −1.19 2.3 hsa-miR-185 9.9 0.53100.0 2.08E−02 −1.22 2.3 hsa-miR-29a 12.6 0.27 100.0 4.99E−03 −1.26 2.4hsa-miR-365 6.1 0.55 71.4 1.93E−02 −1.26 2.4 hsa-miR-628 7.2 0.59 100.02.38E−02 −1.26 2.4 hsa-miR-24 13.2 0.25 100.0 2.46E−04 −1.27 2.4hsa-miR-191 13.3 0.21 100.0 8.65E−05 −1.29 2.4 hsa-miR-339 6.7 0.44 85.75.70E−03 −1.29 2.5 hsa-miR-551b 4.0 0.43 0.0 4.58E−03 −1.31 2.5hsa-miR-30a-3p 8.7 0.56 100.0 3.86E−02 −1.32 2.5 hsa-miR-103 12.9 0.24100.0 2.16E−04 −1.32 2.5 hsa-miR-130b 8.1 0.58 100.0 2.78E−02 −1.33 2.5hsa-miR-107 12.8 0.24 100.0 1.69E−04 −1.34 2.5 hsa-miR-27b 12.2 0.12100.0 2.45E−04 −1.34 2.5 hsa-miR-376a 4.7 0.37 0.0 1.94E−02 −1.41 2.7hsa-miR-142-5p 6.4 0.54 100.0 1.35E−02 −1.41 2.7 hsa-miR-140 7.4 0.60100.0 2.40E−02 −1.45 2.7 hsa-miR-205 4.3 0.67 0.0 3.78E−02 −1.48 2.8hsa-miR-100 12.1 0.43 100.0 5.64E−03 −1.49 2.8 hsa-miR-489 5.3 0.59 14.31.18E−02 −1.49 2.8 hsa-miR-10b 12.3 0.19 100.0 6.73E−04 −1.53 2.9hsa-miR-106b 10.3 0.67 100.0 1.76E−02 −1.54 2.9 hsa-miR-320 10.6 0.41100.0 1.95E−03 −1.57 3.0 hsa-miR-200b 7.0 0.67 100.0 1.43E−02 −1.61 3.1hsa-miR-19b 8.5 0.52 100.0 7.33E−03 −1.65 3.1 hsa-miR-199b 8.7 0.49100.0 1.25E−02 −1.67 3.2 hsa-miR-148a 11.8 0.28 100.0 4.62E−03 −1.74 3.3hsa-miR-189 5.3 0.44 14.3 1.49E−02 −1.75 3.4 hsa-miR-155 13.7 0.14 100.04.28E−02 −1.76 3.4 hsa-miR-200c 7.8 0.79 100.0 3.03E−02 −1.76 3.4hsa-miR-203 5.8 0.71 28.6 1.73E−02 −1.76 3.4 hsa-miR-425-5p 10.6 0.50100.0 2.14E−03 −1.77 3.4 hsa-miR-197 9.7 0.37 100.0 6.33E−03 −1.77 3.4hsa-miR-505 8.9 0.39 100.0 6.33E−04 −1.80 3.5 hsa-miR-101 7.4 0.82 100.02.30E−02 −1.83 3.6 hsa-miR-126 12.7 0.32 100.0 3.08E−04 −1.85 3.6hsa-miR-186 7.8 0.50 100.0 7.09E−03 −1.86 3.6 hsa-miR-145 13.2 0.19100.0 1.14E−04 −1.86 3.6 hsa-miR-30e-3p 8.7 0.39 100.0 3.32E−04 −1.923.8 hsa-miR-497 7.6 0.28 57.1 4.94E−05 −2.04 4.1 hsa-miR-15a 11.7 0.35100.0 5.61E−04 −2.06 4.2 hsa-miR-342 13.6 0.19 100.0 3.53E−04 −2.07 4.2hsa-miR-142-3p 5.2 0.44 14.3 6.95E−04 −2.08 4.2 hsa-miR-486 8.0 0.49100.0 9.10E−04 −2.08 4.2 hsa-miR-22 11.6 0.47 100.0 6.05E−04 −2.11 4.3hsa-miR-199a* 12.8 0.18 100.0 2.43E−06 −2.15 4.4 hsa-miR-484 8.4 0.31100.0 5.59E−05 −2.17 4.5 hsa-miR-194 9.6 0.64 100.0 2.44E−03 −2.20 4.6hsa-miR-331 8.6 0.58 100.0 1.76E−03 −2.21 4.6 hsa-miR-223 11.4 0.61100.0 1.74E−03 −2.24 4.7 hsa-miR-150 13.1 0.30 100.0 1.45E−02 −2.32 5.0hsa-miR-224 8.6 0.83 100.0 6.75E−03 −2.35 5.1 hsa-miR-625 9.2 0.68 100.04.30E−03 −2.36 5.1 hsa-miR-598 7.1 0.70 85.7 5.66E−03 −2.48 5.6hsa-miR-139 9.4 0.45 100.0 1.84E−04 −2.53 5.8 hsa-miR-133a 6.2 0.63 71.49.79E−04 −2.62 6.1 hsa-miR-133b 5.3 0.81 14.3 7.44E−03 −2.64 6.2hsa-miR-629 6.6 0.91 57.1 1.44E−02 −2.65 6.3 hsa-miR-99a 11.4 0.46 100.01.01E−03 −2.74 6.7 hsa-miR-29b 10.8 0.34 100.0 3.23E−05 −2.76 6.8hsa-miR-195 12.9 0.15 100.0 7.00E−06 −2.76 6.8 hsa-miR-452 6.1 1.18 57.11.48E−02 −2.81 7.0 hsa-miR-215 7.0 0.57 100.0 6.95E−04 −2.87 7.3hsa-miR-29c 10.4 0.51 100.0 3.80E−04 −2.88 7.4 hsa-miR-17-3p 6.8 0.6385.7 5.15E−04 −2.89 7.4 hsa-miR-199a 10.2 0.33 100.0 5.92E−05 −2.92 7.5hsa-miR-7 9.8 0.64 100.0 4.08E−04 −3.03 8.2 hsa-miR-218 9.0 0.59 100.02.23E−04 −3.07 8.4 hsa-miR-425-3p 6.5 0.59 42.9 2.67E−04 −3.13 8.7hsa-miR-143 12.7 0.33 100.0 2.34E−05 −3.17 9.0 hsa-miR-192 10.6 0.61100.0 7.77E−05 −4.01 16.1 hsa-miR-95 9.0 0.64 100.0 1.21E−04 −4.16 17.9hsa-miR-335 9.3 0.61 100.0 3.59E−05 −4.21 18.5 hsa-miR-1 9.6 0.84 100.02.32E−04 −4.32 20.0 hsa-miR-31 10.5 1.63 100.0 6.41E−03 −4.64 25.0hsa-asg-5638 3.6 0.77 0.0 1.47E−04 4.27 19.3 hsa-asg-5630 4.0 1.22 14.31.36E−02 3.12 8.7 hsa-asg-11338 4.0 0.36 0.0 3.15E−05 2.68 6.4hsa-asg-9186 5.2 0.53 0.0 2.61E−02 2.61 6.1 hsa-asg-1946 2.7 0.44 0.03.89E−02 2.44 5.4 hsa-asg-14190 2.8 0.55 0.0 4.05E−02 1.99 4.0hsa-asg-11966 3.5 0.96 0.0 2.90E−02 1.97 3.9 hsa-asg-12271 4.5 0.29 0.03.39E−03 1.96 3.9 hsa-asg-381 2.9 0.67 0.0 4.67E−02 1.94 3.8hsa-asg-4051 2.8 0.33 0.0 3.49E−02 1.90 3.7 hsa-asg-8322 2.8 0.12 0.04.54E−02 1.85 3.6 hsa-asg-12810 2.8 0.45 0.0 4.95E−02 1.79 3.5hsa-asg-11238 3.6 0.69 0.0 3.18E−02 1.65 3.1 hsa-asg-10031 4.0 0.60 0.04.73E−02 1.48 2.8 hsa-asg-9858 6.3 0.33 14.3 7.08E−03 1.39 2.6hsa-asg-12381 6.1 0.30 28.6 2.59E−02 1.26 2.4 hsa-asg-2261 3.3 0.33 0.06.01E−03 1.21 2.3 hsa-asg-8633 6.4 0.25 71.4 1.10E−02 1.20 2.3hsa-asg-9903 4.4 0.62 14.3 3.43E−02 1.20 2.3 hsa-asg-13893 5.6 0.20 0.02.48E−02 1.18 2.3 hsa-asg-4780 5.7 0.44 0.0 2.82E−02 1.14 2.2hsa-asg-11850 10.0 0.35 100.0 4.26E−02 1.13 2.2 hsa-asg-8882 6.8 0.12100.0 4.30E−02 1.07 2.1 hsa-asg-4088 4.5 0.50 0.0 3.17E−02 1.00 2.0hsa-asg-12086 5.1 0.26 28.6 4.21E−02 0.96 1.9 hsa-asg-8600 5.2 0.36 0.09.60E−03 0.95 1.9 hsa-asg-8700 5.2 0.32 0.0 1.15E−02 0.94 1.9hsa-asg-8693 5.4 0.34 0.0 2.82E−02 0.94 1.9 hsa-asg-9130 7.2 0.16 100.04.04E−02 0.88 1.8 hsa-asg-12880 7.9 0.14 71.4 2.97E−02 0.79 1.7hsa-cand660 2.8 0.35 0.0 2.65E−02 0.79 1.7 hsa-asg-7252 5.8 0.26 71.42.28E−02 0.75 1.7 hsa-asg-10881 5.8 0.35 57.1 3.40E−02 0.68 1.6hsa-asg-3266 6.7 0.34 100.0 3.39E−02 0.68 1.6 hsa-asg-11906 5.1 0.27 0.02.28E−02 0.64 1.6 hsa-asg-10815 5.9 0.30 14.3 4.31E−02 0.60 1.5hsa-asg-2388 4.7 0.28 0.0 3.94E−02 0.58 1.5 hsa-cand913 12.6 0.14 100.03.47E−03 0.53 1.4 hsa-cand526 4.7 0.16 0.0 1.98E−02 0.51 1.4hsa-asg-4217 6.4 0.26 85.7 4.58E−02 0.51 1.4 hsa-asg-12775 7.4 0.25100.0 4.70E−02 0.48 1.4 hsa-asg-13294 6.3 0.14 85.7 1.38E−02 0.39 1.3hsa-asg-1913 5.6 0.12 14.3 2.67E−02 0.25 1.2 hsa-asg-2826 6.7 0.09 100.01.67E−02 −0.24 1.2 hsa-asg-14025 6.6 0.16 85.7 4.06E−02 −0.30 1.2hsa-asg-6951 7.4 0.12 100.0 6.69E−03 −0.42 1.3 hsa-cand950 6.6 0.13100.0 4.46E−02 −0.42 1.3 hsa-asg-5032 9.4 0.19 100.0 3.29E−02 −0.46 1.4hsa-asg-8672 4.0 0.17 14.3 4.29E−02 −0.46 1.4 hsa-asg-7565 5.5 0.15 28.63.18E−02 −0.53 1.4 hsa-asg-1153 7.2 0.16 100.0 2.74E−03 −0.54 1.5hsa-asg-7569 5.3 0.28 14.3 3.89E−02 −0.54 1.5 hsa-asg-6638 5.6 0.24 85.72.28E−02 −0.55 1.5 hsa-cand109 7.2 0.10 100.0 1.72E−02 −0.57 1.5hsa-asg-1631 5.4 0.30 42.9 3.39E−02 −0.59 1.5 hsa-asg-13143 13.2 0.31100.0 3.78E−02 −0.59 1.5 hsa-asg-4114 5.4 0.29 42.9 2.80E−02 −0.59 1.5hsa-cand407 5.4 0.23 14.3 3.33E−02 −0.59 1.5 hsa-asg-7869 6.2 0.29 100.04.30E−02 −0.60 1.5 hsa-asg-795 5.8 0.26 57.1 4.91E−02 −0.60 1.5hsa-asg-826 8.4 0.22 100.0 3.26E−02 −0.61 1.5 hsa-asg-948 5.9 0.28 57.12.89E−02 −0.62 1.5 hsa-asg-1236 6.0 0.13 42.9 1.21E−02 −0.62 1.5hsa-cand458 5.7 0.29 71.4 4.19E−02 −0.63 1.5 hsa-asg-10619 5.9 0.29 85.73.24E−02 −0.64 1.6 hsa-cand583 5.9 0.22 28.6 2.26E−02 −0.65 1.6hsa-asg-58 5.0 0.27 14.3 1.26E−02 −0.67 1.6 hsa-asg-9815 6.4 0.24 28.67.96E−03 −0.67 1.6 hsa-asg-2615 9.3 0.26 100.0 2.03E−02 −0.69 1.6hsa-asg-1391 7.2 0.28 100.0 4.98E−02 −0.70 1.6 hsa-asg-4433 5.1 0.2728.6 9.87E−03 −0.70 1.6 hsa-asg-1545 5.8 0.18 28.6 3.69E−03 −0.71 1.6hsa-asg-10575 7.6 0.21 100.0 4.09E−02 −0.72 1.6 hsa-asg-2134 4.8 0.2714.3 2.65E−02 −0.72 1.7 hsa-asg-1599 5.1 0.27 14.3 1.23E−02 −0.73 1.7hsa-asg-555 7.2 0.34 100.0 3.98E−02 −0.73 1.7 hsa-asg-1941 4.8 0.22 14.33.41E−02 −0.73 1.7 hsa-asg-2843 5.2 0.22 28.6 6.63E−03 −0.74 1.7hsa-asg-11867 5.0 0.23 14.3 4.34E−03 −0.74 1.7 hsa-asg-9739 5.5 0.29100.0 1.26E−02 −0.74 1.7 hsa-asg-11329 5.2 0.23 14.3 3.43E−02 −0.76 1.7hsa-asg-4196 6.3 0.23 71.4 5.35E−03 −0.77 1.7 hsa-asg-9597 5.1 0.45 28.64.84E−02 −0.79 1.7 hsa-asg-2041 5.5 0.30 14.3 8.94E−03 −0.80 1.7hsa-asg-10914 4.8 0.23 28.6 8.01E−03 −0.80 1.7 hsa-asg-8598 6.0 0.3157.1 1.24E−02 −0.80 1.7 hsa-asg-4587 5.6 0.19 14.3 6.47E−04 −0.81 1.8hsa-asg-7643 8.0 0.43 85.7 4.56E−02 −0.81 1.8 hsa-asg-2693 6.3 0.34 85.71.64E−02 −0.82 1.8 hsa-asg-6651 6.2 0.24 100.0 2.90E−03 −0.83 1.8hsa-asg-8675 6.7 0.33 85.7 2.63E−02 −0.83 1.8 hsa-asg-5576 6.1 0.49 57.14.83E−02 −0.86 1.8 hsa-asg-8477 7.6 0.31 100.0 2.03E−02 −0.87 1.8hsa-asg-6838 6.1 0.16 0.0 2.47E−04 −0.87 1.8 hsa-asg-2989 4.9 0.40 14.32.10E−02 −0.87 1.8 hsa-asg-10796 6.0 0.21 28.6 6.59E−03 −0.89 1.9hsa-asg-6005 7.1 0.33 100.0 3.61E−02 −0.89 1.9 hsa-asg-9562 5.4 0.4742.9 3.95E−02 −0.90 1.9 hsa-asg-12049 7.3 0.28 100.0 1.85E−02 −0.91 1.9hsa-asg-1362 4.6 0.44 14.3 2.93E−02 −0.91 1.9 hsa-asg-9004 8.0 0.30100.0 9.65E−03 −0.91 1.9 hsa-asg-593 4.5 0.28 28.6 3.57E−03 −0.91 1.9hsa-asg-11181 6.0 0.42 14.3 4.20E−02 −0.92 1.9 hsa-asg-6634 4.8 0.44 0.04.70E−02 −0.92 1.9 hsa-asg-3340 6.1 0.39 28.6 3.95E−02 −0.93 1.9hsa-asg-10506 6.1 0.17 57.1 7.50E−03 −0.94 1.9 hsa-asg-11380 3.1 0.340.0 4.85E−02 −0.95 1.9 hsa-cand902 6.1 0.33 28.6 3.52E−02 −0.95 1.9hsa-asg-12460 7.8 0.42 100.0 1.85E−02 −0.96 1.9 hsa-asg-8271 6.4 0.3171.4 5.17E−03 −0.98 2.0 hsa-asg-14186 5.0 0.28 28.6 2.20E−02 −1.00 2.0hsa-asg-10197 7.1 0.48 100.0 3.64E−02 −1.01 2.0 hsa-asg-4599 5.8 0.2057.1 1.81E−02 −1.02 2.0 hsa-asg-3231 11.4 0.27 100.0 5.35E−03 −1.02 2.0hsa-asg-12996 6.8 0.29 100.0 3.58E−03 −1.03 2.0 hsa-asg-10041 5.1 0.4057.1 1.80E−02 −1.03 2.0 hsa-asg-4515 5.2 0.41 14.3 1.87E−02 −1.03 2.0hsa-asg-7471 6.0 0.21 71.4 2.76E−04 −1.05 2.1 hsa-asg-14189 12.0 0.29100.0 2.02E−03 −1.05 2.1 hsa-asg-9530 12.4 0.22 100.0 6.67E−03 −1.06 2.1hsa-asg-2046 4.8 0.34 0.0 1.69E−02 −1.07 2.1 hsa-asg-5467 5.4 0.51 85.73.97E−02 −1.08 2.1 hsa-asg-11096 6.0 0.31 14.3 1.81E−02 −1.09 2.1hsa-miR246 5.7 0.46 71.4 2.27E−02 −1.10 2.1 hsa-asg-1261 5.6 0.27 14.31.49E−02 −1.11 2.2 hsa-asg-10183 5.2 0.30 28.6 7.50E−03 −1.11 2.2hsa-asg-9729 6.7 0.21 100.0 4.49E−04 −1.11 2.2 hsa-cand588 6.7 0.45 85.71.12E−02 −1.14 2.2 hsa-asg-3509 6.0 0.55 42.9 4.26E−02 −1.14 2.2hsa-asg-6949 6.0 0.30 28.6 3.22E−03 −1.14 2.2 hsa-asg-2353 4.7 0.42 14.31.24E−02 −1.14 2.2 hsa-miR-594 8.4 0.65 85.7 4.75E−02 −1.15 2.2hsa-asg-6761 5.1 0.33 71.4 1.15E−02 −1.15 2.2 hsa-asg-11373 7.9 0.4971.4 1.69E−02 −1.16 2.2 hsa-cand190 5.0 0.33 14.3 2.75E−03 −1.16 2.2hsa-asg-9403 8.6 0.42 100.0 1.69E−02 −1.16 2.2 hsa-asg-7275 5.3 0.3371.4 2.46E−02 −1.16 2.2 hsa-asg-6880 5.9 0.37 0.0 3.68E−02 −1.17 2.2hsa-asg-11893 7.9 0.25 100.0 8.95E−04 −1.18 2.3 hsa-asg-271 4.5 0.2514.3 4.07E−03 −1.19 2.3 hsa-asg-8917 7.5 0.56 85.7 3.76E−02 −1.19 2.3hsa-asg-2273 5.1 0.68 28.6 4.91E−02 −1.20 2.3 hsa-asg-11310 6.3 0.40100.0 5.69E−03 −1.20 2.3 hsa-asg-4924 5.4 0.37 42.9 3.25E−03 −1.22 2.3hsa-asg-2750 11.5 0.27 100.0 3.36E−02 −1.22 2.3 hsa-asg-7009 6.2 0.4928.6 3.82E−02 −1.23 2.4 hsa-asg-1305 5.8 0.53 57.1 1.94E−02 −1.24 2.4hsa-asg-7688 6.4 0.25 85.7 8.22E−04 −1.24 2.4 hsa-asg-4469 7.2 0.26100.0 7.37E−03 −1.25 2.4 hsa-miR257 9.2 0.59 100.0 3.10E−02 −1.25 2.4hsa-asg-6457 5.0 0.50 0.0 1.67E−02 −1.26 2.4 hsa-asg-14197_st1 9.7 0.56100.0 1.96E−02 −1.27 2.4 hsa-asg-12666 5.4 0.38 28.6 4.46E−03 −1.28 2.4hsa-asg-6357 5.9 0.49 28.6 4.22E−02 −1.28 2.4 hsa-asg-2824 5.9 0.61 71.42.48E−02 −1.29 2.4 hsa-asg-12339 6.9 0.54 100.0 2.79E−02 −1.29 2.4hsa-asg-12765 5.2 0.62 14.3 2.79E−02 −1.29 2.5 hsa-asg-7319 5.5 0.2057.1 5.22E−04 −1.30 2.5 hsa-asg-3829 4.7 0.42 14.3 2.59E−02 −1.34 2.5hsa-asg-3070 5.3 0.46 14.3 6.36E−03 −1.39 2.6 hsa-asg-2343 5.8 0.36100.0 3.38E−02 −1.39 2.6 hsa-asg-8018 5.3 0.40 14.3 2.41E−03 −1.41 2.7hsa-asg-1268 4.2 0.59 0.0 1.69E−02 −1.41 2.7 hsa-asg-13693 6.1 0.18 42.91.88E−05 −1.41 2.7 hsa-asg-7532 5.7 0.73 57.1 3.46E−02 −1.42 2.7hsa-asg-925 7.2 0.63 100.0 3.20E−02 −1.46 2.8 hsa-asg-3712 6.8 0.60 85.74.08E−02 −1.47 2.8 hsa-asg-12346 7.2 0.50 14.3 5.61E−03 −1.47 2.8hsa-asg-14233 9.4 0.42 100.0 4.31E−03 −1.48 2.8 hsa-asg-13976 5.9 0.2714.3 1.52E−04 −1.49 2.8 hsa-asg-14150 7.0 0.61 14.3 1.26E−02 −1.51 2.8hsa-asg-6717 7.0 0.48 71.4 3.83E−03 −1.51 2.8 hsa-asg-10981 5.3 0.6142.9 1.53E−02 −1.51 2.9 hsa-asg-6247 9.8 0.32 100.0 1.06E−03 −1.52 2.9hsa-asg-7782 6.8 0.76 71.4 3.51E−02 −1.53 2.9 hsa-asg-2365 5.7 0.18 0.06.53E−05 −1.57 3.0 hsa-asg-11896 12.4 0.29 100.0 1.83E−02 −1.58 3.0hsa-asg-3854 5.8 0.23 85.7 3.98E−05 −1.60 3.0 hsa-asg-9508 10.4 0.6085.7 9.09E−03 −1.60 3.0 hsa-asg-13646 7.5 0.48 100.0 2.60E−03 −1.64 3.1hsa-asg-12702 7.3 0.78 100.0 4.87E−02 −1.65 3.1 hsa-cand345 6.1 0.4171.4 1.00E−03 −1.65 3.1 hsa-asg-6413 5.7 0.61 28.6 8.23E−03 −1.68 3.2hsa-asg-9852 6.1 0.30 57.1 3.71E−03 −1.69 3.2 hsa-asg-10473 5.8 0.2028.6 1.22E−05 −1.69 3.2 hsa-asg-12105 5.5 0.62 14.3 2.15E−02 −1.70 3.3hsa-asg-2872 9.8 0.54 100.0 3.76E−03 −1.72 3.3 hsa-asg-8617 6.6 0.6585.7 2.06E−02 −1.72 3.3 hsa-asg-797 5.3 0.14 14.3 4.69E−06 −1.73 3.3hsa-asg-7149 6.3 0.65 71.4 8.91E−03 −1.74 3.3 hsa-asg-12973 6.1 0.3285.7 1.38E−04 −1.78 3.4 hsa-asg-12902 6.8 0.58 100.0 1.15E−02 −1.79 3.5hsa-asg-13097 5.1 1.00 14.3 4.47E−02 −1.83 3.6 hsa-asg-10861 5.9 0.4014.3 5.59E−04 −1.84 3.6 hsa-asg-8682 6.6 0.96 85.7 3.84E−02 −1.86 3.6hsa-asg-13205 7.0 0.81 85.7 1.81E−02 −1.87 3.7 hsa-cand768 11.4 0.53100.0 3.34E−03 −1.87 3.7 hsa-asg-12759 5.1 0.83 57.1 2.37E−02 −1.91 3.8hsa-asg-7080 4.7 0.38 14.3 3.60E−03 −1.92 3.8 hsa-asg-3360 6.9 0.42100.0 1.04E−03 −1.93 3.8 hsa-asg-10149 7.6 0.48 85.7 1.18E−03 −1.95 3.9hsa-asg-11612 5.8 0.66 28.6 5.76E−03 −1.97 3.9 hsa-asg-6138 5.3 0.4728.6 9.12E−04 −1.99 4.0 hsa-asg-13244 6.8 0.51 100.0 2.26E−03 −2.00 4.0hsa-cand965 7.3 0.66 28.6 5.20E−03 −2.00 4.0 hsa-asg-13230 6.8 0.17100.0 7.47E−05 −2.01 4.0 hsa-asg-14245 12.1 0.52 100.0 2.83E−03 −2.034.1 hsa-asg-10263 5.6 0.47 57.1 4.23E−03 −2.05 4.2 hsa-asg-21 6.0 0.7585.7 7.60E−03 −2.06 4.2 hsa-asg-12923 5.9 0.86 57.1 1.56E−02 −2.09 4.3hsa-asg-4002 7.1 0.85 85.7 2.63E−02 −2.09 4.3 hsa-asg-9298 4.5 1.10 14.34.22E−02 −2.10 4.3 hsa-asg-13258 7.9 0.43 100.0 4.64E−04 −2.12 4.3hsa-asg-13260 8.3 0.35 100.0 3.21E−04 −2.13 4.4 hsa-asg-14170 6.4 0.7357.1 1.58E−02 −2.15 4.4 hsa-asg-242 5.7 1.17 42.9 4.82E−02 −2.23 4.7hsa-asg-870 6.1 1.05 42.9 2.66E−02 −2.27 4.8 hsa-asg-4557 7.6 0.40 100.04.94E−04 −2.28 4.8 hsa-asg-5303 7.0 0.53 100.0 2.67E−03 −2.28 4.9hsa-asg-8208 6.1 0.78 28.6 1.86E−02 −2.31 4.9 hsa-miR157 8.9 0.47 100.05.04E−04 −2.32 5.0 hsa-asg-818 5.4 1.17 28.6 4.36E−02 −2.38 5.2hsa-asg-13248 9.2 0.58 100.0 7.91E−04 −2.47 5.5 hsa-asg-562 10.1 0.39100.0 1.88E−04 −2.58 6.0 hsa-asg-13937 8.9 0.62 100.0 4.17E−03 −2.60 6.0hsa-asg-13304 6.6 0.42 85.7 4.40E−04 −2.66 6.3 hsa-asg-13354 8.6 0.80100.0 2.77E−03 −2.67 6.4 hsa-asg-5304 10.3 0.38 100.0 2.95E−05 −2.69 6.4hsa-miR154 5.4 0.89 42.9 6.40E−03 −2.71 6.5 hsa-asg-10883 7.0 0.47 100.02.24E−04 −2.71 6.5 hsa-asg-10387 7.8 1.09 100.0 1.92E−02 −2.74 6.7hsa-asg-10676 6.4 1.03 57.1 7.98E−03 −2.81 7.0 hsa-asg-7074 10.3 0.34100.0 2.02E−03 −2.84 7.2 hsa-asg-13308 10.2 0.44 100.0 5.90E−05 −2.897.4 hsa-asg-10580 7.6 0.93 85.7 4.11E−03 −2.91 7.5 hsa-asg-14160 5.40.72 0.0 1.05E−03 −2.96 7.8 hsa-asg-4205 5.9 1.04 71.4 6.08E−03 −3.008.0 hsa-asg-10666 6.2 0.83 71.4 3.38E−03 −3.01 8.0 hsa-asg-13279 7.40.37 100.0 9.99E−06 −3.17 9.0 hsa-asg-14133 7.7 1.05 100.0 1.12E−02−3.21 9.2 hsa-asg-13237 7.2 0.39 100.0 5.10E−06 −3.62 12.3 hsa-miR-10012.2 0.36 100.0 4.68E−03 −1.40 2.6 hsa-miR-126 12.5 0.24 100.0 1.24E−04−1.93 3.8 hsa-miR-140 7.7 0.70 100.0 2.14E−02 −1.61 3.1 hsa-miR-199a*12.8 0.19 100.0 3.79E−06 −2.13 4.4 hsa-miR-224 8.9 0.67 100.0 5.27E−03−2.03 4.1 hsa-miR-29b 10.9 0.36 100.0 7.70E−05 −2.71 6.6 hsa-miR-331 7.40.44 100.0 2.30E−04 −2.50 5.7 hsa-miR-365 4.9 0.31 0.0 1.73E−03 −1.172.3 hsa-miR-365 6.0 0.73 71.4 6.18E−02 −1.28 2.4 hsa-miR-376a 4.6 0.600.0 5.33E−02 −1.04 2.1 hsa-miR-421 6.8 0.63 85.7 1.84E−01 0.71 1.6hsa-miR-503 7.1 0.60 71.4 5.61E−02 1.03 2.0 hsa-miR-551b 3.8 0.62 0.01.31E−01 −0.85 1.8 hsa-miR-592 3.1 0.30 0.0 1.05E−01 0.79 1.7 SD,standard deviation; %, percentage of samples expressing miRNA abovebackground levels; Log2Diff, difference in VSN-transformed expressionbetween LNposB and LNneg samples.

Example 12 miRNAs Differentially Expressed Between Melanoma Samples andLNneg and between LNpos and LNnet from Melanoma Patients

The inventors identified the common miRNAs that are differentiallyexpressed between melanoma (MEL) samples and cancer-negative lymph nodes(LNneg) and between cancer-positive (LNpos) and cancer-negative (LNneg)lymph nodes from melanoma patients. For this analysis, differentiallyexpressed miRNAs were identified as having a p-value <0.05 andfold-change >2. These miRNAs represent particularly useful biomarkersfor monitoring melanoma metastasis to lymph nodes.

The inventors found 21 miRNAs to be differentially expressed betweenboth MEL and LNneg samples (Table 10) and between LNpos1A and LNnegsamples (Table 11). The miRNAs include hsa-miR-211, -203, -200b, -200c,-509, -513, -429, -187, -132, -605, -183, -508, -493-5p, 421-3p, -506,-584, -576, -495, -182, -514, and -382). Among these, four miRNAs(hsa-miR-203, -211, -509, and -513) had average expression levelsbetween 20- and 160-fold lower in LNneg samples compared to either MEL(Table 10) or LNpos1A samples (Table 11). Among the predicted miRNAs,the inventors identified three (hsa-asg-5638-st2, -11338_st2, and-1296-st1) that were expressed at levels between 5- and 110-fold lowerin LNneg samples compared to either MEL (Table 10) or LNpos1A samples(Table 11).

The inventors found that eight verified miRNAs (hsa-miR-211, -513, -509,-584, -132, -506, -508, and -514) were expressed at lower levels inLNneg samples compared to either MEL (Table 10) or LNposB (Table 12)samples. Among these, three miRNAs (hsa-miR-211, -513, and -509) hadaverage expression levels between 20- and 85-fold lower in LNneg samplesthan in MEL (Table 10) or LNposB (Table 12) samples. Among the predictedmiRNAs, eleven (hsa-asg-5638_st2, 5630_st2, -11338_st2, -9186_st1,-12271_st1, -10031_st1, -12381_st1, -2261_st1, -8633_st1, -4780-st1, and-11850_st1) were expressed at lower levels in LNneg samples compared toMEL (Table 10) or LNposB (Table 12) samples. Among these, four miRNAs(hsa-asg-5638_st2, -5630_st2, -11338_st2, and -9186 st1) had averageexpression levels between 5- and 100-fold lower in LNneg samples than inMEL (Table 10) or LNposB (Table 12) samples.

Together, the inventors found a total of eight verified miRNAs(hsa-miR-211, -513, -509, -584, -132, -506, -508, and -514) that wereexpressed at lower levels in LNneg samples compared to either MEL (Table10), LNpos1A (Table 11), or LNposB (Table 12) samples. Among these, fourmiRNAs (hsa-miR-211, -513, -509, and -132) had average expression levelsbetween 5- and 140-fold lower in LNneg samples than in MEL, LNpos1A, orLNposB samples. The inventors also identified two predicted miRNAs(hsa-asg5638_st2, and -11338_st2) that had average expression levelsbetween 5- and 25-fold lower in LNneg samples than in either MEL,LNpos1A, or LNposB samples.

Example 13 qRT-PCR Verification of Differentially Expressed Micro-RNAsin Melanoma Tissues and Lymph Nodes from Melanoma Patients

The inventors also evaluated the differential expression of 13 selectedmiRNAs in MEL, LNpos, and LNneg frozen biopsy samples (Table 8, Table 9)by qRT-PCR. qRT-PCR reactions were performed using TaqMan® MicroRNAAssays (Applied Biosystems; Foster City, Calif., USA) specific forthirteen of the most differentially expressed miRNAs betweenLNposA/LNposB and LNneg samples and/or between MEL and LNneg samples(hsa-miR-205, -203, -211, -509, -200c, -506, -508, -200b, -514, -584,-429, -592, and -375). Assays were performed according to themanufacturer's instructions with 15 ng of input total RNA from one of 12samples (MEL1, MEL 3, LNneg1-7, LNpos1A, LNpos2B, LNpos3B) (Table 8,Table 9) and were incubated in a 7900HT Fast Real-Time PCR system(Applied Biosystems, Foster City, Calif., USA). Initial data analysiswas done using a 7500 Fast System SDS V2.3 software (AppliedBiosystems). miR-16, whose expression level did not change between thedifferent samples, was used for normalization. Results of qRT-PCRexpression analysis for the thirteen miRNAs were compared with thoseobtained from microarray expression analysis (FIGS. 7-9).

qRT-PCR quantification confirmed the differential expression of tenmiRNAs (hsa-miR-205, -203, -211, -200c, -508, -509, -584, -514, -200b,and -506) between MEL and LNneg samples (FIGS. 7A-7C). These results areconsistent with the results obtained after array analysis (FIGS. 7A-7C).

Upon qRT-PCR analysis of the thirteen miRNAs in LNpos1A and LNnegsamples, twelve were found to be expressed at significantly lower levelsin the LNneg samples and one (hsa-miR-205) was not. These resultscorrespond to those observed with microarray quantification (FIGS.8A-8C).

Upon qRT-PCR analysis of the thirteen miRNAs in LNposB and LNnegsamples, six (hsa-miR-211, -509, -506, -508, -514, and -584) were foundto be expressed at significantly lower levels in the LNneg samples(FIGS. 9A-9C). As observed for the LNpos1A and LNneg samples, miR-205was not found to be expressed at significantly different levels betweenLNposB and LNneg samples. As observed with microarray analysis, miR-429,-592, and -375 were not expressed at significantly different levels inLNposB and LNneg samples when quantified by qRT-PCR. This is in contrastto what was observed for these miRNAs in the LNposA and LNneg samples.In addition, three miRNAs, miR-203, -200b, and -200c, were not founddifferentially expressed between LNposB and LNneg samples, althoughthese miRNAs were found differentially expressed between LNposA andLNneg samples (FIGS. 8A-8C and FIGS. 9A-9C). These observations suggestthat qRT-PCR quantification of miR-200c, -203, -200b and -375 is auseful assay for distinguishing LNposA group lymph nodes from LNposBgroup lymph nodes.

These data demonstrate that both qRT-PCR and microarray analysis areeffective methods for quantifying miRNA expression when evaluatingmetastasis of melanoma to lymph nodes.

Example 14 MicroRNAs Differentially Expressed between Melanoma Samplesand LNpos from Melanoma Patients

Cancer cells present in lymph nodes are considered to be cells arisingfrom a primary tumor, which have metastasized to that node. In the caseof melanoma, a positive lymph node is defined by the AJCC as onecontaining an aggregate of melanoma cells 2 mm or greater in size. It isexpected that miRNA expression in a primary melanoma tumor and inmetastatic inguinal lymph nodes share similarities. However, miRNAexpression differences may also exist as the consequence of geneticalterations linked to the cancer cells' ability to metastasize. Thedifferences in miRNA expression between the primary melanoma and themetastatic melanoma cells in lymph nodes represent clinicallysignificant surrogate markers for the propensity of a primary lesion tometastasize. Such differentially expressed miRNAs also represent targetsfor replacement therapy. To identify any such differences, the inventorscompared miRNA expression between three primary melanoma tumors (MEL)and cancer-positive lymph nodes (LNposA, LNposB), using frozen biopsyspecimens (Table 8, Table 9).

The inventors identified 8 miRNAs (2 verified, 6 predicted) that wereexpressed at significantly lower levels in the LNpos1A sample than inthe MEL samples (p-value<0.05) with at least a 2-fold-change inexpression level (log 2Diff (MEL vs LNpos1A)≧1) (Table 13).Interestingly, hsa-miR-205 was not found significantly differentiallyexpressed by array analysis despite a differential expression of atleast 182-fold between MEL samples and the LNpos1A sample. This resultmay be due to the presence of only one sample in the LNposA group andthe result of a lack of power of the analysis. qRT-PCR analysis,however, confirmed the differential expression between the LNpos1Asample and the MEL samples (FIG. 8A-8C). In addition, a total of 14miRNAs (5 verified and 9 predicted) were identified that were expressedat lower levels in the MEL samples compared to the LNpos1A sample (Table13). Among these, four miRNAs (hsa-miR-215, -592, -518c*, and -522) hadaverage levels of expression between 5- and 25-fold lower in the MELsamples compared to the LNpos1A sample (Table 13).

The inventors also identified 55 miRNAs (29 verified, 26 predicted) withsignificant differential expression between melanoma samples and theLNposB group of cancer-positive lymph nodes (p-value<0.05 and log 2Diff(MEL vs LNposB)≧1) (Table 14). Among the verified miRNAs, two(hsa-miR-205 and -203) had average expression levels at least 500-foldhigher in MEL samples than in LNposB samples and five miRNAs(hsa-miR-31, -200c, -218, -223, and -493-5p) had average expressionlevels between 5- and 60-fold higher in MEL than in LNposB samples(Table 14). Among the 26 predicted miRNAs, only one (hsa-asg-14160-st1)was expressed at least 10-fold higher in MEL samples than in LNposBsamples (Table 14). No verified miRNA was found expressed at a lowerlevel in MEL samples compared to LNposB samples. Only three predictedmiRNAs had average expression levels lower by 2-3-fold in MEL than inLNposB samples (Table 14).

TABLE 13 MicroRNAs Differentially Expressed Between Cancer-PositiveLymph Node Group A Sample (LNpos1A) and Melanoma Samples (MEL). MEL MELvs LNpos1A MicroRNA Probe ID 1 3 Mean SD % LNpos1A LNpos1A % ttestLog2Diff FC hsa-miR-181a* hsa-miR-213_st1 6.17 6.25 6.21 0.05 50 3.58 01.58E−02 2.63 6.2 hsa-asg-9117 hsa-asg-9117_st1 6.98 6.99 6.98 0.01 1005.35 0 5.15E−03 1.64 3.1 hsa-miR-22 hsa-miR-22_st1 11.70 11.79 11.750.06 100 10.32 100 3.35E−02 1.43 2.7 hsa-asg-13881 hsa-asg-13881_st15.86 5.74 5.80 0.09 0 4.41 0 4.87E−02 1.39 2.6 hsa-asg-14175hsa-asg-14175_st1 7.58 7.53 7.55 0.03 100 6.21 100 1.94E−02 1.34 2.5hsa-asg-12885 hsa-asg-12885_st1 6.13 6.11 6.12 0.02 50 4.86 0 1.06E−021.26 2.4 hsa-asg-9128 hsa-asg-9128_st1 5.11 5.05 5.08 0.04 0 4.02 03.23E−02 1.06 2.1 hsa-asg-11458 hsa-asg-11458_st2 6.52 6.52 6.52 0.00100 5.51 0 2.72E−04 1.01 2.0 hsa-asg-3846 hsa-asg-3846_st2 3.89 3.833.86 0.04 0 2.91 0 3.40E−02 0.95 1.9 hsa-asg-954 hsa-asg-954_st1 9.979.90 9.93 0.05 100 9.05 100 4.20E−02 0.88 1.8 hsa-miR-424hsa-miR-424_st1 6.29 6.36 6.32 0.05 100 5.44 0 4.40E−02 0.88 1.8hsa-asg-917 hsa-asg-917_st2 6.00 5.98 5.99 0.02 50 5.20 0 1.92E−02 0.791.7 hsa-asg-6020 hsa-asg-6020_st1 6.77 6.76 6.77 0.00 100 6.00 03.06E−03 0.76 1.7 hsa-asg-11196 hsa-asg-11196_st1 3.62 3.65 3.63 0.02 02.91 0 2.25E−02 0.72 1.6 hsa-asg-8700 hsa-asg-8700_st1 5.57 5.51 5.540.04 0 4.86 0 4.72E−02 0.67 1.6 hsa-asg-12320 hsa-asg-12320_st2 5.655.64 5.65 0.01 50 5.01 0 8.46E−03 0.64 1.6 hsa-cand720 hsa-cand720_st15.98 6.01 6.00 0.02 0 5.38 0 2.57E−02 0.62 1.5 hsa-asg-593hsa-asg-593_st2 4.59 4.61 4.60 0.02 0 4.02 0 2.28E−02 0.58 1.5hsa-asg-11958 hsa-asg-11958_st1 8.16 8.16 8.16 0.01 100 7.60 1008.64E−03 0.56 1.5 hsa-asg-733 hsa-asg-733_st1 6.38 6.41 6.39 0.02 1005.88 100 3.58E−02 0.52 1.4 hsa-asg-4486 hsa-asg-4486_st2 6.38 6.38 6.380.00 100 5.88 100 9.31E−04 0.50 1.4 hsa-asg-9521 hsa-asg-9521_st2 5.475.49 5.48 0.01 100 5.03 0 2.35E−02 0.45 1.4 hsa-asg-9185hsa-asg-9185_st2 8.32 8.33 8.32 0.01 100 7.89 100 1.75E−02 0.44 1.4hsa-asg-8305 hsa-asg-8305_st2 6.82 6.82 6.82 0.00 100 6.40 100 4.72E−030.42 1.3 hsa-asg-8815 hsa-asg-8815_st2 6.03 6.00 6.01 0.02 50 5.59 03.56E−02 0.42 1.3 hsa-asg-2989 hsa-asg-2989_st1 5.07 5.05 5.06 0.01 04.65 0 2.82E−02 0.41 1.3 hsa-asg-13924 hsa-asg-13924_st1 6.05 6.04 6.050.00 50 5.66 0 6.25E−03 0.39 1.3 hsa-asg-8600 hsa-asg-8600_st2 5.62 5.615.62 0.01 50 5.30 0 1.76E−02 0.32 1.2 hsa-asg-3550 hsa-asg-3550_st2 5.965.98 5.97 0.01 50 5.76 0 4.67E−02 0.21 1.2 hsa-asg-9480 hsa-asg-9480_st17.74 7.74 7.74 0.00 100 7.55 100 7.53E−03 0.19 1.1 hsa-asg-11623hsa-asg-11623_st1 9.21 9.21 9.21 0.00 50 9.11 0 8.71E−05 0.10 1.1hsa-let-7b hsa-let-7b_st2 13.69 13.69 13.69 0.00 100 13.63 100 5.54E−030.06 1.0 hsa-asg-12460 hsa-asg-12460_st2 6.63 6.63 6.63 0.00 100 6.68100 1.60E−02 −0.05 1.0 hsa-miR-98 hsa-miR-98_st1 11.73 11.73 11.73 0.00100 11.80 100 4.94E−03 −0.07 1.1 hsa-asg-11293 hsa-asg-11293_st1 6.386.38 6.38 0.00 100 6.46 100 5.32E−03 −0.09 1.1 hsa-asg-11626hsa-asg-11626_st1 6.83 6.83 6.83 0.00 100 6.95 100 1.66E−02 −0.12 1.1hsa-asg-3352 hsa-asg-3352_st1 5.81 5.81 5.81 0.01 50 5.94 100 3.13E−02−0.13 1.1 hsa-asg-4023 hsa-asg-4023_st2 5.91 5.92 5.92 0.00 0 6.06 02.19E−02 −0.15 1.1 hsa-let-7a hsa-let-7a_st2 14.19 14.18 14.18 0.00 10014.34 100 2.00E−02 −0.15 1.1 hsa-asg-13306 hsa-asg-13306_st2 8.90 8.908.90 0.01 100 9.06 100 2.64E−02 −0.16 1.1 hsa-asg-974 hsa-asg-974_st26.60 6.60 6.60 0.00 100 6.77 100 3.83E−03 −0.16 1.1 hsa-asg-7701hsa-asg-7701_st2 5.60 5.59 5.59 0.01 0 5.76 0 3.48E−02 −0.17 1.1hsa-asg-3588 hsa-asg-3588_st1 8.22 8.22 8.22 0.01 100 8.42 100 2.17E−02−0.20 1.1 hsa-asg-2251 hsa-asg-2251_st1 5.37 5.36 5.37 0.01 0 5.59 03.53E−02 −0.23 1.2 hsa-asg-11844 hsa-asg-11844_st2 5.11 5.09 5.10 0.01 05.35 0 4.53E−02 −0.24 1.2 hsa-asg-3340 hsa-asg-3340_st2 5.41 5.43 5.420.01 0 5.66 0 4.44E−02 −0.24 1.2 hsa-miR131 hsa-miR131_st1 5.50 5.525.51 0.01 0 5.76 0 4.31E−02 −0.25 1.2 hsa-asg-10821 hsa-asg-10821_st25.41 5.39 5.40 0.01 0 5.66 0 2.97E−02 −0.26 1.2 hsa-asg-8351hsa-asg-8351_st1 4.94 4.92 4.93 0.02 0 5.20 0 4.97E−02 −0.27 1.2hsa-asg-12169 hsa-asg-12169_st2 6.38 6.38 6.38 0.00 100 6.65 1001.73E−03 −0.27 1.2 hsa-asg-2541 hsa-asg-2541_st1 6.57 6.55 6.56 0.02 1006.84 100 4.41E−02 −0.28 1.2 hsa-asg-9181 hsa-asg-9181_st1 8.97 8.99 8.980.01 50 9.36 0 2.63E−02 −0.37 1.3 hsa-asg-10619 hsa-asg-10619_st1 6.036.00 6.01 0.02 100 6.40 100 3.90E−02 −0.38 1.3 hsa-asg-2626hsa-asg-2626_st1 8.13 8.10 8.11 0.02 100 8.51 100 4.54E−02 −0.40 1.3hsa-asg-8421 hsa-asg-8421_st1 7.98 8.02 8.00 0.02 100 8.42 100 4.47E−02−0.42 1.3 hsa-asg-507 hsa-asg-507_st1 2.58 2.61 2.60 0.02 0 3.03 03.67E−02 −0.43 1.3 hsa-asg-10183 hsa-asg-10183_st1 4.53 4.49 4.51 0.03 04.97 0 4.28E−02 −0.46 1.4 hsa-asg-2500 hsa-asg-2500_st1 6.36 6.35 6.350.01 100 6.81 100 1.19E−02 −0.46 1.4 hsa-cand735 hsa-cand735_st2 7.497.47 7.48 0.01 100 7.95 100 2.09E−02 −0.47 1.4 hsa-asg-14015hsa-asg-14015_st1 5.62 5.60 5.61 0.02 0 6.10 0 2.81E−02 −0.49 1.4hsa-asg-4426 hsa-asg-4426_st2 4.59 4.55 4.57 0.02 0 5.07 0 3.74E−02−0.50 1.4 hsa-miR-519a hsa-miR-519a_st1 5.54 5.52 5.53 0.01 0 6.04 1001.26E−02 −0.51 1.4 hsa-asg-6437 hsa-asg-6437_st1 6.78 6.74 6.76 0.03 1007.34 100 4.22E−02 −0.58 1.5 hsa-asg-14066 hsa-asg-14066_st2 6.81 6.806.81 0.00 100 7.41 100 3.44E−03 −0.60 1.5 hsa-asg-14038hsa-asg-14038_st1 6.44 6.45 6.44 0.01 0 7.05 0 1.49E−02 −0.61 1.5hsa-asg-4148 hsa-asg-4148_st1 2.58 2.61 2.60 0.02 0 3.24 0 2.45E−02−0.64 1.6 hsa-asg-531 hsa-asg-531_st1 2.58 2.61 2.60 0.02 0 3.24 02.45E−02 −0.64 1.6 hsa-asg-4502 hsa-asg-4502_st1 7.20 7.24 7.22 0.03 1007.87 100 3.74E−02 −0.65 1.6 hsa-asg-3808 hsa-asg-3808_st1 7.10 7.09 7.090.01 0 7.77 100 1.36E−02 −0.68 1.6 hsa-cand711 hsa-cand711_st1 9.10 9.139.11 0.02 50 9.79 100 2.48E−02 −0.68 1.6 hsa-asg-8917 hsa-asg-8917_st25.84 5.91 5.87 0.05 0 6.63 0 4.89E−02 −0.75 1.7 hsa-asg-6948hsa-asg-6948_st2 7.69 7.66 7.67 0.02 100 8.46 100 1.72E−02 −0.79 1.7hsa-asg-6149 hsa-asg-6149_st2 6.94 6.90 6.92 0.02 0 7.73 100 2.19E−02−0.81 1.8 hsa-miR198 hsa-miR198_st1 4.90 4.87 4.88 0.02 0 5.70 01.70E−02 −0.82 1.8 hsa-asg-12775 hsa-asg-12775_st1 6.60 6.65 6.63 0.04100 7.45 100 3.53E−02 −0.82 1.8 hsa-asg-13135 hsa-asg-13135_st1 5.345.32 5.33 0.01 0 6.19 100 9.87E−03 −0.85 1.8 hsa-asg-5304hsa-asg-5304_st1 9.09 9.11 9.10 0.01 100 9.96 100 1.03E−02 −0.86 1.8hsa-asg-312 hsa-asg-312_st2 5.65 5.67 5.66 0.01 100 6.53 100 1.19E−02−0.87 1.8 hsa-asg-9526 hsa-asg-9526_st2 4.94 4.87 4.91 0.05 0 5.78 04.47E−02 −0.88 1.8 hsa-asg-3138 hsa-asg-3138_st2 4.59 4.55 4.57 0.02 05.48 0 2.04E−02 −0.91 1.9 hsa-asg-1399 hsa-asg-1399_st1 4.80 4.87 4.830.05 0 5.80 0 4.09E−02 −0.96 1.9 hsa-asg-4599 hsa-asg-4599_st1 5.07 5.055.06 0.01 0 6.03 0 1.19E−02 −0.97 2.0 hsa-asg-7510 hsa-asg-7510_st1 8.628.63 8.62 0.00 100 9.60 100 3.59E−03 −0.98 2.0 hsa-asg-271hsa-asg-271_st1 3.71 3.74 3.73 0.02 0 4.73 0 1.59E−02 −1.00 2.0hsa-asg-2824 hsa-asg-2824_st2 4.85 4.87 4.86 0.02 0 5.86 0 1.22E−02−1.01 2.0 hsa-asg-3941 hsa-asg-3941_st2 5.96 5.89 5.93 0.05 0 7.00 1003.33E−02 −1.07 2.1 hsa-asg-3341 hsa-asg-3341_st1 4.75 4.72 4.73 0.02 05.81 100 1.49E−02 −1.07 2.1 hsa-asg-3832 hsa-asg-3832_st1 4.59 4.61 4.600.02 0 5.71 0 1.18E−02 −1.11 2.2 hsa-asg-10752 hsa-asg-10752_st1 5.155.09 5.12 0.04 0 6.38 100 2.64E−02 −1.26 2.4 hsa-asg-12376hsa-asg-12376_st2 5.30 5.43 5.37 0.09 0 6.87 100 4.51E−02 −1.50 2.8hsa-asg-12246 hsa-asg-12246_st2 4.80 4.89 4.85 0.07 0 6.53 100 3.12E−02−1.68 3.2 hsa-asg-9577 hsa-asg-9577_st1 5.41 5.27 5.34 0.10 0 7.09 1004.34E−02 −1.75 3.4 hsa-asg-12105 hsa-asg-12105_st2 4.34 4.23 4.29 0.08 06.14 0 3.31E−02 −1.85 3.6 hsa-miR-7 hsa-miR-7_st1 8.41 8.58 8.50 0.12100 10.60 100 4.35E−02 −2.10 4.3 hsa-miR-522 hsa-miR-522_st2 3.89 3.653.77 0.17 0 6.53 100 4.85E−02 −2.76 6.8 hsa-miR-518c* hsa-miR-518c-_st23.52 3.45 3.48 0.05 0 6.34 100 1.42E−02 −2.86 7.2 hsa-miR-592hsa-miR-592_st1 3.89 3.65 3.77 0.17 0 6.89 100 4.29E−02 −3.12 8.7hsa-miR-215 hsa-miR-215_st1 5.19 5.59 5.39 0.28 0 9.98 100 4.71E−02−4.59 24.0 hsa-miR-592 hsa-asg-4400_st1 4.53 4.61 4.57 0.06 0 6.79 1002.03E−02 −2.22 4.7 SD, standard deviation; %, percentage of samplesexpressing miRNA above background levels; Log2Diff, difference inVSN-transformed expression between LNpos1A and MEL samples.

TABLE 14 MicroRNAs Differentially Expressed Between Cancer-PositiveLymph Node Group B Samples (LNpos2B, -3B, and -4B) and Melanoma Samples(MEL). MEL LNpos MEL vs LNposB MicroRNA Probe ID 1 3 Mean SD % 2B 3BMean SD % ttest Log2Diff FC) hsa-miR-205 hsa-miR-205_st1 13.32 11.3512.33 1.40 100 2.12 3.51 2.81 0.98 0 1.56E−02 9.52 734.2 hsa-miR-203hsa-miR-203_st2 13.82 12.43 13.13 0.98 100 4.58 3.58 4.08 0.71 08.86E−03 9.04 527.7 hsa-miR-31 hsa-miR-31_st2 12.58 10.88 11.73 1.20 1005.83 5.88 5.86 0.04 50 2.03E−02 5.87 58.5 hsa-miR-200c hsa-miR-200c_st112.67 10.60 11.64 1.47 100 6.68 5.41 6.05 0.90 50 4.42E−02 5.59 48.1hsa-miR-218 hsa-miR-218_st2 8.60 9.75 9.17 0.82 100 5.83 5.93 5.88 0.0750 2.97E−02 3.29 9.8 hsa-miR-223 hsa-miR-223_st1 12.01 11.48 11.75 0.37100 9.03 9.24 9.14 0.15 100 1.18E−02 2.61 6.1 hsa-miR-493-5phsa-miR-493-5p_st1 7.49 7.26 7.38 0.16 100 4.75 4.93 4.84 0.13 03.37E−03 2.53 5.8 hsa-miR-22 hsa-miR-22_st1 11.70 11.79 11.75 0.06 1009.66 9.24 9.45 0.30 100 8.84E−03 2.30 4.9 hsa-miR-425-5phsa-asg-14166_st2 10.59 10.91 10.75 0.23 100 8.77 8.96 8.86 0.13 1009.75E−03 1.89 3.7 hsa-miR-484 hsa-miR-484_st2 7.82 8.42 8.12 0.43 1006.47 6.02 6.24 0.32 0 3.75E−02 1.88 3.7 hsa-miR-127 hsa-miR-127_st1 7.637.11 7.37 0.36 100 5.35 5.72 5.53 0.26 0 2.85E−02 1.84 3.6 hsa-miR-27ahsa-miR-27a_st2 12.18 11.88 12.03 0.21 100 10.16 10.34 10.25 0.13 1009.57E−03 1.78 3.4 hsa-miR-199a* hsa-miR-199a-_st1 12.59 12.36 12.48 0.17100 10.51 10.89 10.70 0.27 100 1.56E−02 1.78 3.4 hsa-miR-24hsa-miR-24_st1 13.74 13.62 13.68 0.08 100 11.97 11.89 11.93 0.05 1001.50E−03 1.75 3.4 hsa-miR-139 hsa-miR-139_st2 8.68 8.59 8.63 0.06 1007.15 6.63 6.89 0.37 100 2.25E−02 1.74 3.3 hsa-miR-365 hsa-miR-365_st16.34 6.81 6.58 0.33 100 4.64 5.08 4.86 0.31 0 3.38E−02 1.71 3.3hsa-miR-7 hsa-miR-7_st1 8.41 8.58 8.50 0.12 100 6.70 6.94 6.82 0.17 1007.55E−03 1.68 3.2 hsa-miR-181a* hsa-miR-213_st1 6.17 6.25 6.21 0.05 504.40 4.74 4.57 0.24 0 1.14E−02 1.64 3.1 hsa-miR-565 hsa-miR-565_st1 7.847.44 7.64 0.28 50 5.95 6.18 6.07 0.16 0 2.06E−02 1.57 3.0 hsa-miR-652hsa-miR-652_st1 9.33 9.38 9.36 0.03 100 8.15 7.62 7.88 0.37 50 3.09E−021.48 2.8 hsa-miR-107 hsa-miR-107_st2 12.74 12.89 12.82 0.11 100 11.5511.28 11.41 0.19 100 1.15E−02 1.40 2.6 hsa-miR-103 hsa-miR-103_st1 12.7912.92 12.86 0.09 100 11.67 11.40 11.54 0.19 100 1.25E−02 1.32 2.5hsa-miR-93 hsa-miR-93_st2 10.97 11.23 11.10 0.18 100 10.10 9.63 9.860.33 100 4.35E−02 1.24 2.4 hsa-miR-324-5p hsa-miR-324-5p_st1 8.42 8.838.62 0.29 100 7.47 7.32 7.39 0.10 100 2.93E−02 1.23 2.3 hsa-miR-152hsa-miR-152_st1 11.10 11.39 11.24 0.20 100 10.07 9.97 10.02 0.07 1001.48E−02 1.22 2.3 hsa-miR-191 hsa-miR-191_st2 13.16 13.38 13.27 0.15 10012.13 11.97 12.05 0.11 100 1.17E−02 1.22 2.3 hsa-miR-17-3phsa-miR-17-3p_st1 4.99 5.01 5.00 0.02 0 3.68 4.13 3.91 0.32 0 4.01E−021.09 2.1 hsa-miR-128b hsa-miR-128b_st1 10.77 10.64 10.71 0.09 100 9.539.86 9.70 0.23 100 2.93E−02 1.01 2.0 hsa-miR-128a hsa-miR-128a_st2 10.3610.22 10.29 0.10 100 9.10 9.49 9.30 0.27 100 4.06E−02 0.99 2.0hsa-let-7b hsa-let-7b_st2 13.69 13.69 13.69 0.00 100 12.79 12.69 12.740.07 100 2.73E−03 0.95 1.9 hsa-miR-214 hsa-miR-214_st1 8.20 8.16 8.180.03 100 7.18 7.51 7.35 0.23 0 3.64E−02 0.83 1.8 hsa-let-7ihsa-let-7i_st1 13.38 13.32 13.35 0.04 100 12.60 12.49 12.54 0.08 1005.81E−03 0.81 1.7 hsa-miR-16 hsa-miR-16_st2 13.40 13.19 13.29 0.15 10012.75 12.63 12.69 0.08 100 3.81E−02 0.60 1.5 hsa-asg-14160hsa-asg-14160_st1 6.21 6.43 6.32 0.15 50 2.77 2.14 2.46 0.45 0 7.41E−033.86 14.6 hsa-asg-13205 hsa-asg-13205_st1 7.87 8.59 8.23 0.51 100 4.955.36 5.15 0.29 0 1.78E−02 3.08 8.5 hsa-asg-13258 hsa-asg-13258_st2 8.928.11 8.52 0.57 100 6.02 5.45 5.74 0.40 0 3.04E−02 2.78 6.9 hsa-asg-14233hsa-asg-14233_st1 10.75 10.40 10.57 0.25 100 7.55 8.37 7.96 0.58 1002.82E−02 2.61 6.1 hsa-asg-13254 hsa-asg-13254_st1 6.05 5.39 5.72 0.46 02.77 3.53 3.15 0.54 0 3.60E−02 2.57 5.9 hsa-asg-14150 hsa-asg-14150_st17.84 8.25 8.04 0.29 100 5.42 5.53 5.48 0.08 0 6.92E−03 2.57 5.9hsa-asg-9723 hsa-asg-9723_st2 5.74 5.05 5.39 0.48 100 3.37 2.61 2.990.54 0 4.27E−02 2.40 5.3 hsa-asg-13646 hsa-asg-13646_st1 8.51 7.77 8.140.53 100 5.91 5.77 5.84 0.10 0 2.61E−02 2.30 4.9 hsa-asg-2365hsa-asg-2365_st1 6.15 6.34 6.25 0.13 50 3.78 4.39 4.08 0.43 0 2.12E−022.16 4.5 hsa-asg-2353 hsa-asg-2353_st1 5.74 5.21 5.47 0.37 0 3.26 3.903.58 0.45 0 4.40E−02 1.90 3.7 hsa-asg-13308 hsa-asg-13308_st2 8.95 9.119.03 0.12 100 7.09 7.50 7.30 0.29 0 1.58E−02 1.73 3.3 hsa-asg-3854hsa-asg-3854_st1 5.71 5.75 5.73 0.03 0 4.11 4.24 4.18 0.09 0 1.84E−031.56 2.9 hsa-asg-5304 hsa-asg-5304_st1 9.09 9.11 9.10 0.01 100 7.67 7.587.63 0.06 50 9.56E−04 1.48 2.8 hsa-asg-8018 hsa-asg-8018_st1 5.41 5.055.23 0.25 0 3.78 4.00 3.89 0.16 0 2.36E−02 1.34 2.5 hsa-asg-12973hsa-asg-12973_st2 5.79 5.46 5.62 0.23 50 4.26 4.34 4.30 0.06 0 1.59E−021.32 2.5 hsa-asg-7688 hsa-asg-7688_st1 6.43 6.38 6.40 0.04 100 4.85 5.435.14 0.41 50 4.94E−02 1.26 2.4 hsa-asg-10913 hsa-asg-10913_st1 6.85 7.016.93 0.12 100 5.75 5.72 5.73 0.02 0 5.19E−03 1.20 2.3 hsa-asg-9980hsa-asg-9980_st1 6.00 5.55 5.78 0.32 50 4.46 4.72 4.59 0.18 0 4.42E−021.19 2.3 hsa-asg-4587 hsa-asg-4587_st1 5.86 6.03 5.95 0.12 50 4.80 4.744.77 0.04 0 5.69E−03 1.18 2.3 hsa-asg-13976 hsa-asg-13976_st2 5.23 5.735.48 0.35 0 4.33 4.39 4.36 0.04 0 4.65E−02 1.12 2.2 hsa-asg-11361hsa-asg-11361_st2 4.90 4.72 4.81 0.12 0 3.58 3.81 3.70 0.16 0 1.63E−021.11 2.2 hsa-asg-10861 hsa-asg-10861_st2 5.27 5.03 5.15 0.17 0 3.87 4.294.08 0.30 0 4.79E−02 1.07 2.1 hsa-asg-2073 hsa-asg-2073_st1 5.62 5.295.46 0.24 0 4.52 4.34 4.43 0.13 0 3.32E−02 1.02 2.0 hsa-asg-2989hsa-asg-2989_st1 5.07 5.05 5.06 0.01 0 4.11 3.98 4.05 0.10 0 4.51E−031.02 2.0 hsa-asg-11458 hsa-asg-11458_st2 6.52 6.52 6.52 0.00 100 5.615.45 5.53 0.12 50 6.66E−03 1.00 2.0 hsa-asg-593 hsa-asg-593_st2 4.594.61 4.60 0.02 0 3.68 3.58 3.63 0.07 0 2.95E−03 0.97 2.0 hsa-asg-11196hsa-asg-11196_st1 3.62 3.65 3.63 0.02 0 2.64 2.74 2.69 0.07 0 2.65E−030.94 1.9 hsa-asg-6717 hsa-asg-6717_st2 6.52 6.25 6.39 0.20 50 5.42 5.475.44 0.04 0 2.15E−02 0.94 1.9 hsa-asg-4924 hsa-asg-4924_st2 4.94 5.255.10 0.22 0 4.26 4.06 4.16 0.14 0 3.70E−02 0.94 1.9 hsa-asg-361hsa-asg-361_st1 6.54 6.49 6.52 0.03 100 5.72 5.49 5.61 0.16 50 1.64E−020.91 1.9 hsa-asg-13370 hsa-asg-13370_st2 5.98 6.09 6.03 0.07 50 5.045.35 5.19 0.22 0 3.59E−02 0.84 1.8 hsa-asg-9741 hsa-asg-9741_st2 6.096.00 6.05 0.06 50 5.35 5.11 5.23 0.17 0 2.46E−02 0.82 1.8 hsa-asg-4433hsa-asg-4433_st1 5.15 5.25 5.20 0.07 0 4.46 4.44 4.45 0.02 0 4.45E−030.75 1.7 hsa-asg-14146 hsa-asg-14146_st1 5.30 5.61 5.46 0.22 0 4.75 4.774.76 0.01 0 4.59E−02 0.70 1.6 hsa-asg-2096 hsa-asg-2096_st1 3.89 3.743.82 0.11 0 3.15 3.16 3.15 0.01 0 1.23E−02 0.67 1.6 hsa-asg-9469hsa-asg-9469_st1 6.25 6.29 6.27 0.03 100 5.61 5.61 5.61 0.00 50 7.37E−040.66 1.6 hsa-asg-360 hsa-asg-360_st2 4.75 4.64 4.69 0.08 0 4.04 4.134.08 0.07 0 1.42E−02 0.61 1.5 hsa-asg-2094 hsa-asg-2094_st1 6.32 6.046.18 0.20 100 5.58 5.57 5.57 0.01 50 4.89E−02 0.61 1.5 hsa-asg-12179hsa-asg-12179_st1 5.96 6.00 5.98 0.03 50 5.52 5.27 5.39 0.18 0 4.36E−020.59 1.5 hsa-asg-58 hsa-asg-58_st1 4.80 4.96 4.88 0.12 0 4.40 4.24 4.320.11 0 3.87E−02 0.56 1.5 hsa-asg-641 hsa-asg-641_st2 6.34 6.15 6.25 0.14100 5.75 5.67 5.71 0.06 50 3.60E−02 0.53 1.4 hsa-miR163 hsa-miR163_st16.41 6.46 6.44 0.04 100 5.88 5.92 5.90 0.03 100 3.69E−03 0.53 1.4hsa-asg-11883 hsa-asg-11883_st1 6.77 6.56 6.66 0.15 100 6.13 6.14 6.140.01 50 3.87E−02 0.52 1.4 hsa-asg-7542 hsa-asg-7542_st2 6.32 6.17 6.250.11 50 5.78 5.75 5.76 0.02 50 2.53E−02 0.48 1.4 hsa-asg-3263hsa-asg-3263_st1 5.46 5.31 5.38 0.11 0 4.85 4.99 4.92 0.10 0 4.62E−020.46 1.4 hsa-asg-4114 hsa-asg-4114_st2 5.19 5.36 5.28 0.12 0 4.85 4.794.82 0.04 0 3.56E−02 0.45 1.4 hsa-asg-10172 hsa-asg-10172_st2 6.54 6.656.60 0.08 100 6.17 6.21 6.19 0.02 100 2.06E−02 0.41 1.3 hsa-asg-13143hsa-asg-13143_st2 12.95 13.00 12.98 0.04 100 12.67 12.51 12.59 0.12 1004.82E−02 0.39 1.3 hsa-asg-6926 hsa-asg-6926_st2 6.13 6.03 6.08 0.07 1005.78 5.76 5.77 0.01 0 2.51E−02 0.31 1.2 hsa-asg-844 hsa-asg-844_st1 3.523.45 3.48 0.05 0 3.26 3.21 3.24 0.03 0 3.07E−02 0.25 1.2 hsa-asg-10276hsa-asg-10276_st1 3.89 3.92 3.91 0.02 0 4.11 4.20 4.16 0.06 0 3.31E−02−0.25 1.2 hsa-miR145 hsa-miR145_st1 5.60 5.55 5.58 0.03 50 5.91 5.815.86 0.06 50 2.96E−02 −0.28 1.2 hsa-asg-3688 hsa-asg-3688_st2 5.07 5.095.08 0.01 0 5.39 5.41 5.40 0.02 50 3.17E−03 −0.32 1.2 hsa-asg-10862hsa-asg-10862_st2 5.30 5.39 5.35 0.06 100 5.70 5.88 5.79 0.13 1004.87E−02 −0.44 1.4 hsa-asg-8600 hsa-asg-8600_st2 5.62 5.61 5.62 0.01 506.15 6.10 6.13 0.04 100 2.92E−03 −0.51 1.4 hsa-asg-9515 hsa-asg-9515_st14.70 4.77 4.73 0.05 0 5.45 5.22 5.34 0.16 50 3.83E−02 −0.60 1.5hsa-asg-11758 hsa-asg-11758_st2 8.77 8.90 8.84 0.09 50 9.38 9.62 9.500.17 50 3.96E−02 −0.66 1.6 hsa-asg-9577 hsa-asg-9577_st1 5.41 5.27 5.340.10 0 5.98 6.12 6.05 0.10 0 1.94E−02 −0.71 1.6 hsa-asg-12376hsa-asg-12376_st2 5.30 5.43 5.37 0.09 0 6.15 6.02 6.09 0.09 50 1.55E−02−0.72 1.6 hsa-asg-6831 hsa-asg-6831_st2 5.91 5.79 5.85 0.08 0 6.53 6.826.68 0.20 50 3.43E−02 −0.82 1.8 hsa-asg-11698 hsa-asg-11698_st2 7.967.83 7.90 0.10 100 8.59 8.93 8.76 0.24 100 4.28E−02 −0.87 1.8hsa-asg-4854 hsa-asg-4854_st2 4.90 5.01 4.95 0.08 0 5.93 5.83 5.88 0.0750 6.71E−03 −0.93 1.9 hsa-asg-12919 hsa-asg-12919_st2 8.37 8.10 8.240.19 100 9.18 9.40 9.29 0.16 100 2.66E−02 −1.05 2.1 hsa-asg-12775hsa-asg-12775_st1 6.60 6.65 6.63 0.04 100 8.08 7.77 7.93 0.22 1001.41E−02 −1.30 2.5 hsa-asg-9903 hsa-asg-9903_st2 3.80 4.00 3.90 0.14 05.61 5.57 5.59 0.03 50 3.55E−03 −1.69 3.2 hsa-miR-199a*hsa-asg-14140_st1 12.66 12.43 12.55 0.16 100 10.49 10.90 10.70 0.29 1001.59E−02 1.85 3.6 hsa-miR-365 hsa-cand708_st1 5.74 5.98 5.86 0.17 503.58 3.81 3.70 0.16 0 5.82E−03 2.16 4.5 hsa-miR-365 hsa-asg-13156_st16.38 6.80 6.59 0.30 100 4.26 5.18 4.72 0.65 0 6.66E−02 1.86 3.6 SD,standard deviation; %, percentage of samples expressing miRNA abovebackground levels; Log2Diff, difference in VSN-transformed expressionbetween LNposB and MEL samples.

Example 15 Colon Carcinoma Samples and RNA Isolation

FirstChoice® human total RNA from nine normal (cancer-negative) lymphnode (cat#AM7894) and four normal colon (cat# AM7986) specimens waspurchased from Ambion (Ambion, Inc). Seven cancer-positive lymph nodesfrom colon cancer patients were purchased from ProteoGenex (ProteoGenex,Inc.) (Table 15). Total RNA isolation was performed, using the mirVanamiRNA Isolation Kit (Ambion, Inc.), following the manufacturer'sprotocol. Purified total RNA was quantified using a Nanodrop® ND-1000(Nanodrop Technologies).

TABLE 15 Patient and tissue pathology information for sevencancer-positive lymph node samples from patients with metastatic coloncancer. TNM Stage (Sobin and Wittekind, 2002). Age Overall Lymph(yr)/Sex, TNM Stage Node Sample Ethnicity Histological Diagnosis StageGroup Metastases LNpos4 55/Male adenocarcinoma moderately to poorlyT3N2M0 III 6/8 lymph Caucasian differentiated nodes LNpos5 64/Maleadenocarcinoma moderately T3N1M1 IV 2/6 lymph Caucasian differentiatednodes LNpos6 74/Male adenocarcinoma moderately T3N2M0 III 4/6 lymphCaucasian differentiated nodes LNpos7 73/Male adenocarcinoma moderatelyto poorly T3N1M0 III 2/6 lymph Caucasian differentiated nodes LNpos859/Female adenocarcinoma well to poorly T3N1M0 III 3/5 lymph Caucasiandifferentiated nodes LNpos9 75/Female adenocarcinoma moderately T3N2M0III 4/4 lymph Caucasian differentiated nodes LNpos12 67/Femaleadenocarcinoma well to moderately T3N2M1 IV 4/4 lymph Caucasiandifferentiated nodes

Example 16 miRNAs Differentally Expressed Between Normal Colon Tissueand Cancer-negative Lymph Nodes

To identify miRNAs that may be useful markers for metastasis of coloncancer to lymph nodes, the inventors identified miRNAs that areexpressed at significantly higher levels in normal colon tissue samplesthan in cancer-negative lymph node samples.

miRNAs that are differentially expressed between normal colon (NCo)samples and cancer-negative lymph node (LNneg) samples, both describedabove in Example 15, were determined using one of two microarrayplatforms: mirVana™ miRNA Bioarrays V1.0 (Ambion) or the DiscovArray™miRNA Expression Service (Asuragen). The DiscovArray™ miRNA ExpressionService uses the miRChip V1.0 (described in Example 2 above).

mirVana Bioarrays. The mirVana V1.0 Bioarrays contain 377 individualmiRNA probes, including 281 human miRNAs from Sanger database (V.6), 33novel human miRNAs (Ambi-miR5), and 63 mouse or rat miRNAs from themiRBase Sequence Database (microma.sanger.ac.uk) (Griffiths-Jones etal., 2006). miRNA expression analysis was performed as describedpreviously (Shingara et al., 2005), with the following modifications.microRNA-containing fractions were purified from 10 μg of total RNA,using a flashPAGE™ Fractionation Apparatus (Ambion). Purified small RNAswere labeled with Cy5 (GE Healthcare Life Sciences, Piscataway, N.J.,USA) and hybridized to microarrays according to the manufacturer'sinstructions. Following hybridization, the arrays were scanned using theAxon® GenePix 400B scanner and associated GenePix software (MolecularDevices Corporation, Sunnyvale, Calif.).

mirVana Bioarray data processing. Raw array data were normalized withthe variance stabilization method (VSN) (Huber et al., 2002). VSN is aglobal normalization process that stabilizes the variance evenly acrossthe entire range of expression and utilizes calibration of signalfollowed by transformation of data to a generalized natural logarithmicspace in lieu of the traditional logarithm base 2 transformation.Absolute values and differences in VSN-transformed expression aredenoted by H and ΔH, respectively, and were used for all subsequent dataanalyses. Differences in normalized expression values between samples(ΔH) can be transformed to a generalized fold-change via exponentiationbase e. These values will exhibit a compression for small differences inexpression. For each array, the minimum observable threshold wasdetermined by examining the foreground minus background medianintensities for ‘EMPTY’ spots. The minimum threshold was defined as 5%symmetric trimmed mean plus 2 standard deviations across all ‘EMPTY’spots on an individual array. For an overview of miRNA array processingand analysis, see Davison et al., (2006).

Additional data analyses. A one-way analysis of variance (ANOVA) modelwas used to test the hypothesis that there was no difference inexpression between groups of samples for each miRNA on the array.Pair-wise comparisons for differentially expressed miRNAs identified byANOVA were then performed to measure relative differences. For each pairof sample types (normal colon and lymph nodes), a two-sample t-testassuming equal variance was carried out for every miRNA and multiplicitycorrection was included to control the false discovery rate at 0.05%(Davison et al., 2006).

miRChip Microarrays. microRNA purification and labeling and miRChipexpression analysis were performed as described above in Example 2.

In a preliminary study, miRNA expression was determined for three normalcolon (NCo) samples and three cancer-negative lymph node (LNneg)samples, using the mirVana Bioarray platform. The inventors identified20 miRNAs whose average expression levels in the three cancer-negativelymph node samples were significantly lower than their averageexpression levels in the normal colon samples (p-value<0.05; foldchange≧5) (Table Co2). Among these, six miRNAs (hsa-miR-200b, -200a,-200c, -141, -215, and -375) had average expression levels that were atleast 100-fold lower in the cancer-negative lymph node sample. SevenmiRNAs (hsa-miR-192, -194, -429, -203, -187, and -1 and ambi-miR-7072)had average expression levels that were between 10- and 100-fold lowerin the cancer-negative lymph node samples. Seven other miRNAs hadaverage expression levels that were between 5- and 10-fold lower incancer-negative lymph node samples.

TABLE 16 miRNAs identified with mirVana Bioarrays as beingdifferentially expressed between normal colon (NCo) and cancer-negativelymph nodes (LNneg). ΔH NCo p-value Mean Mean vs Fold NCo vs microRNA %NCo % LNneg NCo LNneg LNneg Change LNneg hsa-miR-200b 100 33 9.47 3.146.33 561 1.69E−06 hsa-miR-200a 100 0 8.26 2.02 6.23 509 2.83E−04hsa-miR-200c 100 100 9.70 4.75 4.95 142 6.41E−05 hsa-miR-141 100 33 8.053.13 4.93 138 9.94E−04 hsa-miR-215 100 67 8.25 3.38 4.87 130 8.07E−03hsa-miR-375 100 0 7.00 2.26 4.73 114 1.81E−04 hsa-miR-192 100 100 10.385.78 4.60 99 7.28E−04 hsa-miR-194 100 67 10.25 6.17 4.08 59 2.46E−03hsa-miR-429 100 0 7.26 3.29 3.97 53 1.35E−05 hsa-miR-203 100 0 6.79 3.293.49 33 8.15E−05 hsa-miR-187 100 100 5.65 2.28 3.37 29 7.41E−04ambi-miR-7072 100 33 4.82 2.11 2.71 15 1.05E−03 hsa-miR-1 100 100 8.375.79 2.58 13 6.87E−03 hsa-miR-133a 67 0 8.94 6.88 2.05 8 2.40E−03hsa-miR-9 67 0 3.96 1.99 1.97 7 2.10E−02 hsa-miR-137 100 100 3.90 1.981.92 7 9.29E−03 hsa-miR-10a 100 100 9.56 7.72 1.84 6 8.60E−04hsa-miR-422a 100 100 7.95 6.37 1.57 5 6.78E−04 hsa-miR-124a 100 0 4.142.58 1.57 5 8.34E−03 hsa-miR-422b 100 100 9.00 7.44 1.57 5 1.93E−04

The miRNAs identified as differentially expressed between normal colonsamples and cancer-negative lymph nodes represent useful biomarkers forindicating the presence of metastatic colon cancer cells in lymph nodes.

Independently from this experiment, the inventors also performed miRNAexpression profiling and comparison with four stage III (T3N2MX) coloncancer samples and four normal colon samples, using the same microarrayplatform. The results revealed that none of the miRNAs, identified asdifferentially expressed between these normal colon and colon cancersamples were among the miRNAs most differentially expressed betweencancer-negative lymph nodes and colon cancer-positive lymph nodes (datanot shown). This observation suggests that, at least in the case ofcolon cancer, the most differentially expressed miRNAs between normalcolon and normal lymph node samples are also representative of the mostdifferentially expressed miRNAs between cancer-positive lymph nodes andcancer-negative lymph nodes.

Next, using the miRChip microarray, the inventors determined miRNAexpression for four normal colon samples and nine cancer-negative lymphnodes (including the three normal colon samples and the threecancer-negative lymph nodes analyzed on mirVana Bioarrays in thepreliminary study described immediately above).

The inventors identified 200 miRNAs whose average expression levels weresignificantly different between the normal colon samples (NCo) and thecancer-negative lymph node samples (LNneg) (t-test (LNneg vs NCo)<0.05)(Table 17). Fifty of those miRNAs (21 verified, 29 predicted) hadaverage expression levels that were at least 5-fold lower in thecancer-negative lymph node samples than in the normal colon samples(LNneg vs NCo p-value<0.05; log 2Diff NCo vs LNneg≧2.35). Among theverified miRNAs, hsa-miR-375 and -429 had average expression levels thatwere at least 100-fold lower in cancer-negative lymph node samples.Hsa-miR-215 and -200a had average expression levels that were between50- and 70-fold lower in cancer-negative lymph node samples. Ten miRNAs(hsa-miR-200b, -200c, -141, -194, -203, -200a*, 605, -192, -187, and-133b) had average expression levels that were 10- to 50-fold lower incancer-negative lymph node samples. Seven miRNAs (hsa-miR-133a, -338,-378, -9, -487b, -495, and -582) had average expression levels that werebetween 5- and 10-fold lower in cancer-negative lymph node samples.Among the predicted miRNAs, seven (hsa-asg-14153_st2, -14182_st1,-7637_st2, 11715_st2, -12227_st1, -924_st1 and -10131_st2) had averageexpression levels that were between 10- and 53-fold lower incancer-negative lymph node samples than in normal colon samples.Twenty-two predicted miRNAs had average expression levels that werebetween 5- and 10-fold lower in cancer-negative lymph node samples thanin normal colon samples.

TABLE 17 MicroRNAs Differentially Expressed Between Normal Colon Samples(NCo) and Cancer-Negative Lymph Node Samples (LNneg). NCo LNneg MicroRNAProbe ID 1 2 3 4 Mean SD % 1 2 3 4 5 6 7 8 9 Mean SD % ttest log2DiffFold hsa-miR-375 hsa-miR-375_st1 12.4 13.4 11.5 13.6 12.7 0.97 100 6.485.40 6.75 3.46 3.57 4.22 1.61 5.17 6.87 4.84 1.78 0 4.88E−06 7.92 241.8hsa-miR-429 hsa-miR-429_st1 11.0 11.1 9.87 10.4 10.6 0.60 100 4.03 2.294.23 2.80 3.02 3.30 2.91 3.08 5.88 3.51 1.07 0 9.57E−08 7.11 137.9hsa-miR-215 hsa-miR-215_st1 11.9 13.3 10.7 13.1 12.3 1.20 100 7.48 6.276.23 7.17 6.05 5.34 6.12 5.65 5.72 6.23 0.70 33 1.49E−07 6.07 67.3hsa-miR-200a hsa-miR-200a_st2 12.0 12.6 11.0 12.3 12.0 0.71 100 6.795.54 7.01 6.23 5.80 5.10 4.67 5.32 8.26 6.08 1.12 67 1.06E−06 5.94 61.4hsa-asg-14153 hsa-asg- 9.4 9.10 8.35 9.45 9.09 0.52 75 3.68 2.91 3.653.60 3.02 3.30 1.77 3.39 4.91 3.36 0.83 0 7.05E−08 5.73 53.0 14153_st2hsa-miR- hsa-miR- 13.7 14.0 13.3 14.0 13.8 0.34 100 8.14 8.24 9.42 7.207.32 7.35 7.12 8.18 10.9 8.21 1.25 100 3.07E−06 5.61 48.9 200b 200b_st1hsa-asg- hsa-asg- 8.5 8.62 5.81 7.60 7.65 1.32 75 3.10 1.76 2.60 2.523.24 2.48 3.38 2.56 1.52 2.57 0.63 0 1.00E−06 5.08 33.8 14182 14182_st1hsa-asg- hsa-asg- 9.4 9.29 8.73 9.53 9.24 0.36 100 4.24 3.16 5.01 4.034.48 4.55 3.88 4.21 5.76 4.37 0.73 0 7.60E−08 4.87 29.3 7637 7637_st2hsa-miR- hsa-miR- 14.0 14.6 13.6 14.5 14.2 0.46 100 10.1 9.29 10.8 8.688.64 8.61 8.59 9.41 12.0 9.57 1.20 100 1.35E−05 4.66 25.3 200c 200c_st1hsa-miR-141 hsa-miR- 9.0 10.6 7.64 9.23 9.16 1.24 100 5.60 4.34 5.464.96 4.92 3.44 4.45 4.39 5.05 4.73 0.66 0 3.31E−06 4.42 21.5 141_st1hsa-miR- hsa-miR- 13.3 14.9 12.5 13.9 13.7 1.00 100 10.3 9.81 9.43 9.859.44 8.82 9.17 8.74 9.53 9.46 0.51 100 4.87E−07 4.26 19.2 194 194_st2hsa-miR- hsa-miR- 11.8 10.8 11.6 11.2 11.4 0.42 100 7.84 6.34 7.73 8.746.95 5.90 5.80 6.60 9.84 7.31 1.36 78 1.20E−04 4.10 17.2 203 203_st2hsa-miR-200a* hsa-miR-200a-_st2 8.0 7.78 6.67 7.35 7.46 0.60 100 3.683.46 2.84 4.46 3.24 2.48 2.74 3.78 3.51 3.36 0.61 0 2.18E−07 4.10 17.2hsa-miR- hsa-miR- 7.0 7.43 6.85 7.26 7.14 0.25 100 3.26 2.83 3.65 1.522.45 2.83 3.52 3.66 4.98 3.19 0.96 0 6.92E−06 3.95 15.5 605 605_st1hsa-asg- hsa-asg- 6.3 6.91 6.10 5.76 6.29 0.49 75 2.57 3.00 2.84 2.242.78 2.48 1.24 2.19 3.95 2.59 0.73 0 1.72E−06 3.70 13.0 11715 11715_st2hsa-asg- hsa-asg- 7.0 6.58 5.06 6.71 6.34 0.87 50 2.38 1.59 2.84 1.962.62 2.66 3.38 3.24 3.35 2.67 0.62 0 2.74E−06 3.67 12.7 12227 12227_st1hsa-asg- hsa-asg- 5.3 10.8 4.52 9.82 7.62 3.17 50 5.20 4.79 3.97 4.993.31 3.37 2.74 3.39 4.07 3.98 0.86 0 6.52E−03 3.63 12.4 924 924_st1hsa-miR- hsa-miR- 13.5 14.2 13.0 14.1 13.7 0.53 100 11.3 10.3 10.6 10.510.0 9.19 9.94 9.33 10.1 10.1 0.66 100 1.18E−06 3.60 12.1 192 192_st2hsa-miR- hsa-miR- 6.4 9.31 4.82 9.47 7.52 2.26 75 5.30 3.39 3.38 4.333.80 3.93 3.65 3.96 3.74 3.94 0.59 0 7.08E−04 3.58 12.0 187 187_st1hsa-miR- hsa-miR- 11.4 9.69 10.3 10.9 10.5 0.74 100 7.88 6.67 5.01 10.67.95 6.69 7.83 5.01 5.88 7.07 1.78 67 3.28E−03 3.52 11.5 133b 133b_st2hsa-asg- hsa-asg- 6.3 7.69 6.89 7.67 7.16 0.64 100 3.92 3.72 4.10 3.153.17 3.82 3.23 3.78 5.59 3.83 0.75 0 9.42E−06 3.32 10.0 10131 10131_st2hsa-miR- hsa-miR-133a_st1 12.0 10.5 11.1 11.6 11.3 0.64 100 8.29 8.156.45 11.3 8.69 7.61 8.83 6.78 6.96 8.12 1.47 89 1.53E−03 3.25 9.5 133ahsa-asg- hsa-asg- 6.4 8.04 6.16 7.67 7.07 0.92 100 3.68 3.16 5.34 3.073.17 3.37 3.77 4.47 4.68 3.86 0.80 0 5.12E−05 3.21 9.3 1296 1296_st1hsa-asg- hsa-asg- 6.3 6.74 5.77 6.62 6.37 0.43 75 3.10 3.31 2.60 2.622.70 3.15 3.52 3.24 4.19 3.16 0.50 0 2.82E−07 3.21 9.2 5119 5119_st1hsa-asg- hsa-asg- 6.5 6.68 5.39 6.64 6.31 0.62 75 3.41 2.83 4.57 2.432.86 3.00 0.98 3.78 4.50 3.15 1.10 0 2.56E−04 3.16 9.0 10247 10247_st1hsa-asg- hsa-asg- 5.9 5.61 5.45 6.82 5.95 0.61 75 3.41 3.00 3.28 2.982.37 2.83 2.21 2.74 4.07 2.99 0.56 0 3.35E−06 2.97 7.8 12139 12139_st2hsa-asg- hsa-asg-3822_st2 6.0 6.73 5.63 6.68 6.27 0.53 50 2.75 3.59 4.232.71 3.10 2.83 2.21 3.53 5.29 3.36 0.94 0 1.33E−04 2.91 7.5 3822hsa-asg- hsa-asg- 6.5 6.35 5.54 6.10 6.13 0.44 100 2.75 3.16 4.23 3.152.20 3.44 3.23 3.08 4.30 3.28 0.66 0 8.39E−06 2.85 7.2 13908 13908_st2hsa-asg- hsa-asg-9980_st1 6.3 7.71 7.20 7.77 7.26 0.65 100 5.71 4.974.57 2.43 3.96 3.44 4.45 5.61 5.17 4.48 1.07 11 5.70E−04 2.78 6.9 9980hsa-asg- hsa-asg- 6.4 7.55 6.26 7.69 7.00 0.73 100 5.60 4.06 5.01 4.693.96 1.96 3.65 4.63 4.50 4.23 1.03 0 5.44E−04 2.77 6.8 14208 14208_st1hsa-asg- hsa-asg- 5.8 5.65 5.26 5.86 5.66 0.29 75 2.38 1.76 4.35 2.983.31 3.00 0.98 3.78 3.81 2.93 1.07 0 4.58E−04 2.74 6.7 13705 13705_st1hsa-asg- hsa-asg- 6.6 6.59 5.96 6.99 6.54 0.43 100 3.92 4.58 3.47 3.603.51 3.82 3.38 3.39 4.68 3.82 0.50 0 1.25E−06 2.73 6.6 8174 8174_st1hsa-asg- hsa-asg- 5.5 9.09 5.29 10.1 7.51 2.48 50 4.79 5.48 4.17 4.835.96 5.68 3.52 3.72 4.94 4.79 0.85 0 1.11E−02 2.72 6.6 4209 4209_st2hsa-miR- hsa-miR- 7.3 9.13 5.57 8.29 7.58 1.53 100 5.64 4.58 6.09 5.384.58 2.83 5.38 4.01 5.40 4.88 1.00 11 2.66E−03 2.70 6.5 338 338_st1 hsa-hsa- 6.1 6.72 5.32 5.96 6.03 0.57 50 3.68 2.65 3.47 3.60 2.94 3.30 3.522.74 4.07 3.33 0.47 0 2.17E−06 2.70 6.5 cand852 cand852_st2 hsa-asg-hsa-asg- 5.7 5.85 5.59 5.92 5.78 0.14 25 4.03 2.47 2.36 2.24 2.94 2.483.52 3.39 4.50 3.10 0.80 0 4.68E−05 2.68 6.4 4219 4219_st1 hsa-miR-hsa-miR- 7.5 8.10 6.55 7.42 7.41 0.65 75 5.25 4.94 5.37 6.89 5.97 2.836.77 4.16 1.42 4.85 1.80 22 1.99E−02 2.57 5.9 378 378_st1 hsa-miR-9hsa-miR-9_st2 8.1 5.32 5.96 6.44 6.46 1.20 100 4.03 4.06 3.47 3.86 4.192.83 4.09 3.90 4.68 3.90 0.51 0 1.68E−04 2.56 5.9 hsa-miR-9hsa-cand90_st2 7.4 5.21 4.52 5.83 5.75 1.25 25 4.14 3.72 3.28 3.60 3.452.83 3.23 2.37 4.19 3.42 0.58 0 6.36E−04 2.33 5.0 hsa-asg- hsa-asg- 6.46.44 6.11 6.50 6.37 0.18 75 3.41 3.72 3.97 3.67 3.51 3.30 3.08 4.11 5.643.82 0.75 0 4.15E−05 2.55 5.8 11160 11160_st2 hsa-asg- hsa-asg- 6.3 6.246.41 7.09 6.52 0.38 100 3.41 4.06 3.97 3.86 4.19 3.93 4.45 3.24 4.833.99 0.49 0 1.79E−06 2.53 5.8 11361 11361_st2 hsa- hsa- 5.2 6.25 5.586.04 5.79 0.44 25 3.55 2.47 3.81 3.92 3.38 1.63 4.19 3.08 3.35 3.26 0.790 1.05E−04 2.53 5.8 cand318 cand318_st1 hsa-asg- hsa-asg- 5.4 6.24 5.226.20 5.78 0.52 50 4.33 3.16 2.84 3.31 3.38 3.93 2.39 2.91 3.16 3.27 0.580 1.28E−05 2.52 5.7 5190 5190_st1 hsa-asg- hsa-asg- 5.6 5.21 5.32 6.465.67 0.56 50 2.38 3.46 3.47 2.43 2.70 2.66 3.38 3.24 4.68 3.15 0.72 07.25E−05 2.51 5.7 4978 4978_st2 hsa- hsa- 7.4 8.91 6.50 9.29 8.03 1.30100 6.64 6.07 6.56 4.62 4.24 5.25 5.89 4.63 5.95 5.54 0.89 22 1.79E−032.50 5.6 cand26 cand26_st2 hsa-miR- hsa-miR- 8.7 8.33 8.34 8.95 8.590.31 100 6.59 6.30 7.48 7.41 6.76 5.89 5.51 4.30 4.91 6.13 1.08 671.10E−03 2.47 5.5 487b 487b_st2 hsa-asg- hsa-asg- 5.7 6.58 5.20 6.355.96 0.62 25 4.03 2.83 3.97 3.39 3.75 2.83 3.88 3.66 3.16 3.50 0.47 06.90E−06 2.46 5.5 3469 3469_st2 hsa- hsa- 3.1 8.36 3.05 7.59 5.53 2.8450 4.03 3.31 2.36 3.73 1.87 2.31 4.37 2.56 3.16 3.08 0.86 0 3.13E−022.45 5.5 cand317 cand317_st1 hsa-miR-495 hsa-miR-495_st1 8.9 8.07 7.478.44 8.23 0.61 100 6.54 5.29 7.48 6.77 6.82 4.94 5.76 3.08 5.40 5.791.32 56 5.22E−03 2.44 5.4 hsa-miR-582 hsa-miR-582_st1 8.1 6.95 7.69 7.097.47 0.57 100 6.37 2.83 5.08 6.47 5.41 4.62 4.67 4.89 5.17 5.06 1.07 221.53E−03 2.41 5.3 hsa-asg- hsa-asg- 7.5 8.99 6.35 9.13 8.00 1.31 1006.15 6.12 5.76 5.43 5.31 5.25 5.76 5.01 6.09 5.65 0.42 22 3.72E−04 2.355.1 13135 13135_st1 hsa-miR-411 hsa-miR-411_st1 7.8 7.03 5.57 6.46 6.720.96 75 5.39 4.25 5.08 5.75 6.07 3.58 3.99 2.91 2.77 4.42 1.21 116.64E−03 2.30 4.9 hsa-miR- hsa-miR- 6.8 6.09 5.29 5.73 6.00 0.67 50 3.413.00 4.76 3.97 4.75 3.58 4.09 2.01 3.81 3.71 0.86 0 6.68E−04 2.29 4.9381 381_st2 hsa-asg- hsa-asg- 5.3 6.61 5.19 6.37 5.87 0.73 50 3.81 3.315.01 2.80 3.17 2.83 2.39 4.01 5.05 3.60 0.95 0 1.44E−03 2.27 4.8 280_st1280_st1 hsa-asg-1596 hsa-asg-1596_st2 6.8 7.41 6.88 7.05 7.04 0.26 1004.98 5.58 4.57 3.80 4.92 4.69 4.74 4.76 4.91 4.77 0.47 0 2.16E−06 2.274.8 hsa-asg- hsa-asg- 5.7 5.21 4.78 6.60 5.59 0.79 50 4.33 3.46 4.103.15 3.45 2.66 3.38 2.37 3.16 3.34 0.62 0 1.61E−04 2.25 4.7 1290712907_st2 hsa-asg- hsa-asg- 7.6 8.78 6.12 9.09 7.91 1.34 100 6.25 5.506.58 5.95 5.31 5.95 4.81 5.57 5.72 5.74 0.52 22 1.18E−03 2.17 4.5 1088110881_st1 hsa-miR-1 hsa-miR-1_st1 12.8 12.7 12.2 12.8 12.6 0.29 100 11.611.2 8.69 12.2 11.2 10.4 10.7 8.18 9.95 10.4 1.35 100 9.88E−03 2.16 4.5hsa-asg- hsa-asg- 8.1 7.24 6.46 7.41 7.33 0.71 100 5.71 4.91 7.51 6.576.42 4.08 5.20 3.66 2.77 5.20 1.52 22 2.41E−02 2.12 4.4 5021 5021_st1hsa-miR- hsa-miR- 10.0 14.4 9.18 13.6 11.8 2.60 100 10.9 10.2 9.46 10.69.21 9.07 8.33 9.20 10.5 9.74 0.88 100 4.67E−02 2.09 4.3 31 31_st2hsa-miR- hsa-miR- 8.4 7.72 8.08 7.63 7.96 0.36 100 6.60 6.25 5.46 7.746.68 5.05 5.55 4.11 5.45 5.88 1.06 44 3.24E−03 2.08 4.2 189 189_st1hsa-miR- hsa-miR- 9.8 9.40 7.61 8.56 8.84 0.97 100 7.01 6.97 8.21 8.588.20 5.73 6.24 4.55 5.59 6.79 1.38 67 2.18E−02 2.06 4.2 368 368_st2hsa-asg- hsa-asg- 5.4 6.12 5.42 5.96 5.73 0.36 75 3.68 2.65 4.10 4.083.45 2.66 3.99 3.39 5.11 3.68 0.77 0 3.92E−04 2.05 4.2 548 548_st1hsa-asg- hsa-asg- 7.2 7.55 7.02 7.83 7.40 0.36 100 6.60 5.14 5.40 5.435.18 5.10 5.42 4.55 5.34 5.35 0.54 11 2.95E−05 2.05 4.1 12666 12666_st2hsa-miR-9* hsa-miR-9-_st2 8.8 7.73 7.34 8.53 8.12 0.71 100 6.72 6.566.09 6.62 5.92 6.35 5.55 5.27 5.72 6.09 0.51 44 1.01E−04 2.04 4.1hsa-miR-493-5p hsa-miR-493- 7.5 6.47 8.24 6.77 7.25 0.79 100 4.92 4.347.40 6.69 6.83 4.22 4.99 2.56 5.11 5.23 1.52 33 3.12E−02 2.02 4.1 5p_st1hsa-miR- hsa-asg- 7.3 3.95 6.99 3.81 5.53 1.92 50 3.68 3.84 4.23 2.432.37 3.30 3.65 2.37 5.92 3.53 1.13 11 3.57E−02 2.00 4.0 592 4400_st1hsa-asg- hsa-asg- 4.4 5.52 3.48 5.66 4.78 1.02 50 3.26 3.31 3.28 2.713.02 2.48 2.03 2.28 2.77 2.79 0.46 0 4.12E−04 1.98 4.0 12107 12107_st1hsa-asg- hsa-asg- 11.6 11.3 10.9 11.0 11.2 0.29 100 9.14 9.03 9.21 9.919.27 8.84 9.82 9.28 8.97 9.28 0.37 100 1.30E−06 1.97 3.9 3998 3998_st1hsa-asg- hsa-asg- 7.6 7.98 7.94 7.94 7.87 0.17 25 7.62 6.85 4.23 6.076.96 7.03 4.77 5.17 4.40 5.90 1.28 0 1.24E−02 1.97 3.9 12815 12815_st2hsa-asg-6277 hsa-asg-6277_st2 8.7 7.74 9.19 8.30 8.50 0.62 100 7.41 7.435.60 6.46 6.99 7.49 5.55 6.87 5.08 6.54 0.92 67 2.76E−03 1.96 3.9hsa-asg- hsa-asg- 7.5 7.34 7.03 8.26 7.55 0.52 100 6.28 6.44 5.31 5.776.00 6.74 3.65 5.70 4.45 5.59 0.99 22 3.74E−03 1.96 3.9 14148 14148_st2hsa-miR- hsa-miR- 7.0 6.54 5.19 5.37 6.03 0.89 50 4.33 3.16 4.85 4.905.43 3.70 3.77 2.01 4.50 4.07 1.04 0 7.78E−03 1.96 3.9 154 154_st2hsa-asg- hsa-asg- 8.5 8.13 7.68 8.83 8.30 0.51 100 6.82 6.23 6.75 7.006.46 5.34 6.40 5.22 6.96 6.35 0.66 78 2.89E−04 1.95 3.9 14146 14146_st1hsa-miR-299-5p hsa-miR-299- 8.1 7.11 6.25 7.29 7.19 0.77 100 6.25 4.976.33 6.89 6.50 3.58 5.66 3.08 4.50 5.31 1.35 44 2.61E−02 1.89 3.7 5p_st2hsa-miR-7 hsa-miR-7_st1 10.5 11.7 10.1 11.5 11.0 0.78 100 9.97 8.68 10.29.12 8.69 8.18 9.27 9.10 8.96 9.13 0.64 100 7.34E−04 1.89 3.7 hsa- hsa-4.7 5.81 3.27 5.42 4.81 1.12 50 2.38 3.00 3.07 3.23 3.02 2.83 3.38 3.721.71 2.93 0.59 0 1.82E−03 1.88 3.7 cand825 cand825_st1 hsa-asg-3912hsa-asg-3912_st1 4.6 4.19 4.78 5.73 4.83 0.65 25 2.38 3.16 3.47 2.332.86 1.96 3.52 2.83 4.07 2.95 0.67 0 6.58E−04 1.88 3.7 hsa-miR- hsa-miR-5.5 5.52 4.15 5.92 5.29 0.78 25 5.15 3.46 3.81 3.60 3.45 2.13 2.57 2.743.81 3.41 0.88 0 3.79E−03 1.88 3.7 577 577_st1 hsa-miR- hsa-miR- 12.011.6 11.2 11.4 11.5 0.36 100 9.61 9.46 9.68 10.3 9.48 9.33 10.3 9.769.62 9.73 0.35 100 2.72E−06 1.86 3.6 422a 422a_st1 hsa-asg- hsa-asg- 5.96.15 6.14 6.23 6.12 0.11 100 5.30 3.84 4.57 4.69 4.06 4.31 4.45 2.914.19 4.26 0.66 0 1.84E−04 1.86 3.6 1268 1268_st1 hsa-miR- hsa-miR- 7.25.47 7.34 5.30 6.33 1.09 50 3.10 5.71 4.71 4.46 4.40 5.99 4.28 2.28 5.374.48 1.20 0 2.31E−02 1.85 3.6 654 654_st1 hsa-miR- hsa-asg- 6.4 5.325.36 5.28 5.60 0.57 25 2.38 3.72 4.35 4.28 4.95 3.00 4.28 3.39 3.67 3.780.79 0 1.66E−03 1.82 3.5 329 13589_st2 hsa-miR- hsa-asg- 6.7 5.47 4.825.69 5.69 0.81 50 4.65 3.46 4.76 3.97 4.78 4.03 4.60 3.24 4.07 4.17 0.570 2.38E−03 1.51 2.9 329 13204_st2 hsa-miR-329 hsa-miR-329_st1 6.6 4.964.63 5.50 5.43 0.88 25 4.33 3.59 5.15 3.86 5.10 3.44 3.38 3.90 3.35 4.010.70 0 9.72E−03 1.42 2.7 hsa-asg- hsa-asg- 8.0 7.30 6.36 7.26 7.25 0.69100 6.12 5.83 7.17 6.40 6.41 4.55 5.34 3.08 3.95 5.43 1.33 56 2.72E−021.82 3.5 13254 13254_st1 hsa-asg- hsa-asg- 11.1 7.52 11.4 8.25 9.60 2.00100 7.38 8.68 6.81 6.89 8.24 8.69 7.04 8.08 8.27 7.79 0.76 100 3.24E−021.81 3.5 8922 8922_st2 hsa-miR-127 hsa-miR-127_st1 9.5 8.74 7.80 9.318.84 0.76 100 7.33 6.03 8.47 8.04 8.19 6.20 7.20 6.02 5.93 7.05 1.03 561.02E−02 1.79 3.5 hsa-asg- hsa-asg- 4.7 4.96 4.22 5.62 4.88 0.58 75 2.753.46 1.88 3.31 3.45 2.66 3.23 3.24 3.95 3.10 0.60 0 4.06E−04 1.78 3.42485 2485_st2 hsa-asg-10775 hsa-asg- 7.1 7.28 6.49 7.35 7.06 0.39 1005.64 5.24 5.34 5.58 4.89 5.18 5.42 5.07 5.23 5.29 0.24 0 5.86E−07 1.773.4 10775_st2 hsa-asg-6642 hsa-asg-6642_st2 5.8 6.51 5.29 6.27 5.98 0.5325 4.58 4.34 4.93 4.42 4.28 4.08 3.52 3.53 4.30 4.22 0.46 0 7.53E−051.76 3.4 hsa-asg- hsa-asg- 10.6 9.77 10.8 10.2 10.3 0.48 100 9.35 9.767.61 8.73 9.25 9.68 7.70 8.16 7.34 8.62 0.94 100 5.09E−03 1.76 3.4 58105810_st2 hsa-miR- hsa-asg- 8.0 7.50 6.06 6.96 7.13 0.83 100 5.56 5.386.61 6.07 5.86 4.62 4.45 4.70 5.17 5.38 0.72 11 2.65E−03 1.75 3.4 376a10049_st1 hsa-miR- hsa-miR- 7.4 6.91 5.85 6.44 6.65 0.67 75 5.68 4.586.02 6.18 5.37 4.55 3.08 4.39 4.30 4.90 0.99 0 8.86E−03 1.75 3.4 376a376a_st2 hsa-miR-376a hsa-asg-14234_st1 6.5 5.89 5.29 4.96 5.67 0.69 503.92 4.79 3.97 4.54 5.37 4.22 3.52 4.63 2.56 4.17 0.82 0 8.65E−03 1.502.8 hsa-asg- hsa-asg- 6.4 6.59 6.70 6.56 6.57 0.10 100 4.98 5.42 4.104.19 5.55 3.82 5.20 5.69 4.59 4.84 0.69 0 4.64E−04 1.74 3.3 1404814048_st1 hsa-miR-18a hsa-miR-18a_st1 7.6 9.28 6.66 7.52 7.77 1.10 1006.30 6.48 7.62 6.48 5.05 4.66 6.29 6.20 5.40 6.05 0.89 44 1.18E−02 1.723.3 hsa-asg- hsa-asg- 10.7 9.89 11.0 10.4 10.5 0.48 100 9.73 9.81 7.939.00 9.47 9.59 8.13 8.34 7.31 8.81 0.91 100 5.03E−03 1.71 3.3 67286728_st2 hsa-miR- hsa-miR- 5.8 6.76 4.52 3.55 5.16 1.41 50 5.15 3.953.47 3.73 4.19 3.15 2.21 2.01 3.16 3.45 0.97 0 2.61E−02 1.71 3.3 19a19a_st1 hsa-asg- hsa-asg- 3.2 6.85 3.05 6.07 4.81 1.94 25 3.92 3.31 2.602.98 3.75 3.00 3.23 2.56 2.77 3.12 0.48 0 2.66E−02 1.68 3.2 92539253_st2 hsa-asg- hsa-asg- 7.6 7.61 7.23 7.23 7.43 0.23 50 5.92 6.625.60 6.49 5.31 6.18 3.65 6.02 5.97 5.75 0.89 0 3.85E−03 1.68 3.2 40154015_st2 hsa-asg- hsa-asg- 9.0 6.02 8.50 5.54 7.29 1.77 75 4.72 7.074.46 5.47 5.58 6.60 4.93 5.49 6.21 5.62 0.87 22 3.84E−02 1.68 3.2 74397439_st1 hsa-asg- hsa-asg- 7.4 6.89 6.28 6.58 6.81 0.51 100 5.39 5.245.08 5.64 5.08 4.69 5.58 4.89 4.68 5.14 0.35 0 2.66E−05 1.67 3.2 128128_st2 hsa-asg- hsa-asg- 9.3 7.98 9.05 8.53 8.72 0.60 100 8.16 8.005.65 7.70 7.86 8.17 6.53 6.40 5.20 7.07 1.15 67 2.16E−02 1.65 3.1 1217212172_st2 hsa-miR-124a hsa-miR-124a_st1 6.6 6.02 5.51 5.89 6.03 0.49 504.86 4.72 4.35 5.34 4.40 3.93 2.74 4.21 4.91 4.38 0.75 0 2.12E−03 1.643.1 hsa-miR- hsa- 6.2 5.45 4.89 6.25 5.71 0.67 25 4.14 4.34 4.35 4.994.24 2.83 2.91 4.01 5.34 4.13 0.83 0 6.55E−03 1.58 3.0 124a cand79_st1hsa-miR- hsa-miR- 13.0 12.9 12.6 12.7 12.8 0.20 100 11.1 11.0 11.2 11.811.2 10.8 11.5 11.2 11.0 11.2 0.30 100 7.30E−07 1.64 3.1 422b 422b_st2hsa-asg- hsa-asg- 6.0 7.05 7.17 6.48 6.69 0.51 75 5.35 5.88 4.17 4.934.58 5.66 4.45 5.30 5.14 5.05 0.57 0 4.44E−04 1.64 3.1 3614 3614_st1hsa-asg- hsa-asg- 5.6 5.37 4.52 4.49 5.01 0.60 25 4.33 3.84 4.46 4.194.01 3.30 2.03 2.19 2.34 3.41 0.98 0 1.22E−02 1.60 3.0 5617 5617_st1hsa-asg-3251 hsa-asg-3251_st1 5.3 6.35 4.63 6.60 5.72 0.92 50 4.79 4.654.57 3.46 4.10 4.47 3.52 3.66 3.81 4.12 0.52 0 1.82E−03 1.60 3.0hsa-asg- hsa-asg- 3.8 7.02 2.87 5.73 4.86 1.87 25 4.03 1.76 3.81 3.803.63 3.44 3.65 2.74 2.56 3.27 0.75 0 4.48E−02 1.59 3.0 9280 9280_st1hsa-asg-8902 hsa-asg-8902_st2 7.2 6.02 7.50 6.77 6.88 0.64 75 6.82 6.244.80 4.80 5.64 5.59 5.15 4.80 3.88 5.30 0.88 22 8.62E−03 1.57 3.0hsa-asg- hsa-asg- 4.0 5.92 5.04 5.73 5.18 0.85 25 3.92 4.42 3.28 2.983.24 4.62 2.91 4.39 2.77 3.62 0.73 0 5.87E−03 1.56 3.0 11688 11688_st1hsa-miR-410 hsa-miR-410_st1 5.7 4.74 4.34 4.13 4.74 0.71 25 3.10 3.002.12 4.28 3.17 3.44 3.77 2.56 3.16 3.18 0.63 0 2.20E−03 1.56 2.9hsa-asg- hsa-asg- 7.9 7.74 7.60 7.63 7.72 0.14 100 6.12 6.18 6.20 7.256.21 5.79 5.86 5.53 6.58 6.19 0.50 56 9.79E−05 1.53 2.9 7471 7471_st1hsa-asg- hsa-asg- 6.8 5.92 4.68 6.13 5.90 0.91 75 5.04 4.25 4.46 4.284.36 4.22 4.81 4.39 3.51 4.37 0.42 0 1.32E−03 1.53 2.9 1301 1301_st1hsa-asg- hsa-asg- 8.1 7.42 8.45 7.64 7.91 0.46 100 6.39 7.00 5.31 6.726.61 7.40 5.51 6.45 6.14 6.39 0.67 33 1.82E−03 1.51 2.9 9053 9053_st1hsa-miR- hsa-miR- 7.1 5.81 7.14 7.28 6.85 0.70 75 5.09 5.89 6.45 6.046.28 6.16 4.09 4.83 3.35 5.35 1.09 22 2.90E−02 1.50 2.8 432 432_st2 hsa-hsa- 9.0 8.42 9.58 8.66 8.92 0.50 100 7.96 8.53 6.18 7.45 7.89 8.52 6.517.08 6.74 7.43 0.86 89 8.60E−03 1.49 2.8 cand545 cand545_st1 hsa-miR-18bhsa-miR-18b_st1 7.5 8.89 6.52 7.40 7.58 0.98 100 6.70 6.07 7.22 6.525.60 5.18 6.32 5.41 6.09 6.12 0.65 44 8.30E−03 1.46 2.8 hsa-asg-hsa-asg- 6.0 4.70 4.31 4.90 5.00 0.76 25 4.14 3.46 3.47 4.86 3.96 3.302.39 2.19 4.19 3.55 0.86 0 1.48E−02 1.45 2.7 3910 3910_st2 hsa-asg-hsa-asg- 9.7 9.76 10.1 9.83 9.88 0.20 50 9.37 9.77 7.69 8.42 8.87 9.337.52 7.95 7.10 8.45 0.94 22 1.33E−02 1.43 2.7 1751 1751_st2 hsa-asg-hsa-asg- 9.6 9.30 9.84 9.69 9.61 0.23 0 8.93 9.21 6.75 8.01 8.64 9.386.92 8.06 7.80 8.19 0.94 11 1.37E−02 1.43 2.7 4869 4869_st1 hsa-asg-67hsa-asg-67_st2 5.3 5.77 4.00 6.99 5.53 1.23 75 5.09 3.84 4.57 2.98 3.804.13 4.28 4.11 4.19 4.11 0.58 0 1.38E−02 1.42 2.7 hsa-asg- hsa-asg- 10.59.96 10.6 9.92 10.2 0.39 100 9.45 9.92 7.79 9.19 9.80 9.64 8.28 8.367.33 8.86 0.94 100 1.64E−02 1.41 2.7 388 388_st1 hsa-miR- hsa-miR- 8.26.96 8.29 7.84 7.83 0.62 100 7.24 6.62 5.65 6.34 6.64 6.63 6.17 6.575.93 6.42 0.47 56 7.79E−04 1.41 2.7 566 566_st1 hsa-asg- hsa-asg- 6.75.09 6.26 5.66 5.93 0.71 50 4.14 4.06 2.36 5.75 5.00 4.76 4.87 4.76 5.054.53 0.95 0 2.40E−02 1.41 2.7 2027 2027_st1 hsa-miR-96 hsa-miR-96_st15.2 5.77 4.15 5.89 5.26 0.80 25 5.52 3.95 3.65 3.60 4.65 3.44 3.65 2.563.67 3.85 0.83 11 1.55E−02 1.41 2.6 hsa-asg- hsa-asg- 11.1 10.9 11.512.2 11.4 0.57 100 10.4 10.9 9.42 9.81 10.4 10.9 9.24 9.87 9.15 10.00.70 100 4.80E−03 1.40 2.6 9078 9078_st2 hsa-asg- hsa-asg- 9.1 9.05 9.389.36 9.25 0.16 100 8.97 8.76 6.70 8.31 8.74 8.71 7.10 7.37 5.97 7.851.09 89 2.97E−02 1.40 2.6 5423 5423_st2 hsa-asg- hsa-asg- 5.6 5.15 5.006.18 5.49 0.53 50 4.58 3.84 4.93 4.76 4.32 3.70 3.23 3.53 4.07 4.11 0.580 1.97E−03 1.38 2.6 1221 1221_st2 hsa-asg- hsa-asg- 8.3 7.41 8.21 7.767.93 0.43 100 7.28 7.85 5.49 6.82 7.44 7.25 6.12 5.43 5.32 6.55 0.98 562.27E−02 1.38 2.6 4116_st2 4116_st2 hsa-asg- hsa-asg- 10.1 10.0 10.310.1 10.1 0.16 100 9.52 9.94 7.67 8.96 9.51 9.77 8.30 8.24 7.24 8.790.97 33 1.92E−02 1.37 2.6 5379 5379_st2 hsa-asg- hsa-asg- 8.5 8.08 8.978.39 8.48 0.37 100 7.65 8.28 6.07 6.86 7.71 7.86 6.29 6.95 6.37 7.120.79 89 7.59E−03 1.37 2.6 11521 11521_st1 hsa-asg- hsa-asg- 9.3 9.379.50 9.11 9.34 0.16 100 8.79 8.62 6.92 8.26 8.22 8.17 7.34 8.26 7.197.97 0.66 100 2.12E−03 1.36 2.6 12925 12925_st1 hsa-miR-369-5phsa-miR-369- 6.1 6.02 4.08 5.23 5.38 0.97 50 4.14 3.31 5.01 4.90 4.893.15 3.52 3.24 4.07 4.03 0.76 0 1.91E−02 1.36 2.6 5p_st2 hsa-miR-hsa-miR- 9.1 8.68 7.60 8.47 8.46 0.63 100 7.04 6.87 7.63 7.47 7.54 7.076.96 6.67 6.71 7.11 0.36 100 3.95E−04 1.35 2.6 379 379_st1 hsa- hsa-10.4 10.3 10.9 10.3 10.5 0.29 100 10.0 10.0 8.14 9.43 9.78 9.92 8.318.96 7.78 9.16 0.89 44 1.40E−02 1.35 2.6 cand711 cand711_st1 hsa-miR-hsa-miR- 10.1 10.2 9.64 10.6 10.1 0.43 100 10.2 9.01 8.85 8.85 9.08 8.558.63 8.96 7.36 8.84 0.74 89 6.41E−03 1.34 2.5 132 132_st1 hsa-asg-hsa-asg- 7.0 5.32 5.45 5.66 5.86 0.79 25 5.04 3.95 5.40 5.47 4.65 4.694.19 2.74 4.59 4.52 0.84 0 2.06E−02 1.34 2.5 13166 13166_st2 hsa-asg-hsa-asg- 11.4 11.0 12.0 11.6 11.5 0.40 100 11.0 11.1 9.12 10.5 10.9 10.99.67 10.0 8.53 10.22 0.94 89 2.13E−02 1.33 2.5 6234 6234_st1 hsa-miR-183hsa-miR-183_st1 8.2 8.87 8.83 9.33 8.82 0.45 100 8.17 7.21 7.22 8.438.06 6.32 8.13 6.85 7.13 7.50 0.72 89 6.81E−03 1.31 2.5 hsa-asg-hsa-asg- 5.6 5.89 5.39 6.10 5.76 0.30 50 4.92 4.50 4.23 4.46 4.48 5.003.99 4.21 4.40 4.47 0.32 0 2.99E−05 1.30 2.5 10830 10830_st2 hsa-asg-hsa-asg- 8.3 6.65 9.14 8.34 8.11 1.05 100 7.48 7.62 6.26 6.77 7.18 7.006.14 7.10 5.84 6.82 0.62 67 1.64E−02 1.29 2.4 5326 5326_st2 hsa-asg-hsa-asg- 11.3 11.4 11.9 11.1 11.4 0.36 100 10.8 10.9 9.49 10.2 10.7 10.89.68 9.81 8.83 10.16 0.75 100 8.13E−03 1.29 2.4 7096 7096_st2 hsa-asg-hsa-asg- 9.6 7.78 9.24 7.87 8.64 0.95 100 7.87 8.13 6.25 6.87 7.60 7.816.96 7.87 7.06 7.38 0.62 89 1.49E−02 1.26 2.4 7070 7070_st1 hsa-asg-hsa-asg- 7.2 7.08 7.88 6.64 7.21 0.51 100 6.12 6.47 5.74 5.65 6.08 5.985.38 5.87 6.29 5.95 0.33 11 2.29E−04 1.26 2.4 1689 1689_st2 hsa-asg-hsa-asg- 6.5 5.09 6.56 5.13 5.84 0.85 25 4.79 5.64 2.48 4.42 4.78 5.204.74 4.66 4.63 4.59 0.87 0 3.50E−02 1.25 2.4 7226 7226_st1 hsa-asg-hsa-asg- 5.8 4.89 4.22 4.40 4.84 0.73 25 4.33 3.46 3.28 3.97 4.06 3.443.52 3.53 2.77 3.59 0.47 0 3.11E−03 1.24 2.4 12690 12690_st1 hsa-asg-53hsa-asg- 10.3 10.6 11.1 10.3 10.6 0.38 100 10.0 9.85 8.64 9.44 10.1 10.08.86 9.06 8.13 9.36 0.73 100 9.12E−03 1.24 2.4 53_st2 hsa-asg- hsa-asg-7.3 7.05 7.38 7.05 7.20 0.18 75 6.89 6.93 5.25 6.55 6.45 6.53 5.15 5.154.87 5.97 0.85 22 1.71E−02 1.23 2.3 1153 1153_st1 hsa-asg- hsa-asg- 11.010.8 11.7 10.8 11.0 0.42 100 10.5 10.5 8.59 9.98 10.7 10.6 9.33 9.908.62 9.87 0.84 89 2.05E−02 1.22 2.3 12136 12136_st2 hsa-asg- hsa-asg-10.9 10.3 11.2 10.5 10.7 0.42 100 9.98 10.4 8.69 9.59 10.3 10.2 8.869.42 8.38 9.55 0.77 67 1.35E−02 1.22 2.3 12289 12289_st1 hsa-asg-hsa-asg- 7.6 6.66 7.51 7.25 7.27 0.44 75 6.15 6.88 4.97 6.26 6.54 7.184.93 6.11 5.52 6.06 0.79 11 1.65E−02 1.21 2.3 1898 1898_st1 hsa-asg-hsa-asg- 6.2 5.97 5.29 6.08 5.89 0.41 25 5.30 4.50 4.10 4.86 5.15 4.354.99 4.30 4.59 4.68 0.41 0 4.85E−04 1.21 2.3 10336 10336_st1 hsa-miR-137hsa-miR-137_st1 6.0 3.95 4.28 3.81 4.53 1.06 25 3.26 3.00 3.97 2.62 3.023.93 2.74 3.90 3.51 3.33 0.52 0 1.65E−02 1.21 2.3 hsa-asg- hsa-asg- 11.110.5 11.4 10.8 11.0 0.37 100 10.7 10.6 9.11 9.71 10.1 10.4 9.08 9.608.63 9.80 0.76 89 1.30E−02 1.20 2.3 11514 11514_st1 hsa-asg- hsa-asg-9.4 9.51 9.72 9.67 9.59 0.12 100 9.15 9.69 7.02 8.67 9.00 9.31 7.70 7.867.14 8.39 0.99 100 3.75E−02 1.20 2.3 5189 5189_st2 hsa-asg- hsa-asg- 5.96.18 5.19 5.69 5.75 0.42 50 4.72 3.95 4.46 6.07 5.10 4.69 3.38 3.53 5.054.55 0.84 0 2.26E−02 1.20 2.3 8380 8380_st2 hsa-asg- hsa-asg- 8.0 7.488.20 7.81 7.87 0.31 100 6.92 7.90 6.04 6.68 6.50 7.31 6.29 6.42 6.106.68 0.60 56 3.71E−03 1.19 2.3 3150 3150_st1 hsa- hsa- 7.5 7.67 7.638.44 7.82 0.42 100 6.59 7.38 6.43 6.01 6.51 6.94 6.36 6.72 6.78 6.640.39 33 4.19E−04 1.18 2.3 miR203 miR203_st1 hsa-asg-511 hsa-asg-511_st16.4 7.15 5.48 6.43 6.37 0.68 75 5.78 4.72 5.94 5.21 4.58 4.91 5.66 5.364.59 5.20 0.52 0 5.81E−03 1.17 2.3 hsa-asg- hsa-asg- 11.2 10.9 11.5 11.411.2 0.24 100 11.1 10.6 9.55 10.1 10.7 10.7 9.58 9.90 8.65 10.1 0.78 1001.51E−02 1.17 2.2 6398 6398_st2 hsa-asg- hsa-asg- 7.8 7.88 7.58 7.917.81 0.15 100 7.37 7.65 5.90 7.03 7.42 7.58 5.80 5.49 5.59 6.65 0.93 563.33E−02 1.16 2.2 1375 1375_st1 hsa-asg- hsa-asg- 10.9 10.5 11.4 11.011.0 0.35 100 10.6 10.6 9.48 9.73 10.3 10.4 9.25 9.85 8.36 9.87 0.76 891.68E−02 1.15 2.2 9092 9092_st2 hsa-asg- hsa-asg- 7.2 6.45 8.29 7.227.30 0.76 100 6.64 6.80 6.07 5.58 6.48 6.85 5.76 5.63 5.61 6.16 0.54 339.22E−03 1.15 2.2 5735 5735_st2 hsa-miR- hsa-miR- 8.9 7.40 8.45 8.748.38 0.68 100 7.69 7.30 7.86 7.63 7.93 7.04 6.90 6.46 6.30 7.23 0.60 781.10E−02 1.14 2.2 382 382_st1 hsa-asg- hsa-asg- 9.1 8.86 9.19 8.77 8.990.21 100 8.59 8.83 6.61 8.28 8.59 8.30 7.23 7.30 6.89 7.85 0.83 442.28E−02 1.14 2.2 9920 9920_st1 hsa-asg- hsa-asg- 10.0 9.50 10.3 9.819.93 0.37 100 9.61 9.60 7.78 8.55 9.30 9.63 7.84 8.83 7.98 8.79 0.78 1001.97E−02 1.14 2.2 9266 9266_st1 hsa-asg- hsa-asg- 8.7 7.92 8.34 8.008.25 0.37 100 6.62 7.76 6.46 6.43 7.41 7.60 6.71 7.50 7.46 7.11 0.54 672.83E−03 1.14 2.2 14051 14051_st2 hsa-miR- hsa- 5.1 5.52 3.48 5.58 4.940.99 25 4.03 3.16 4.23 4.69 4.28 3.44 4.19 2.37 3.81 3.80 0.71 03.59E−02 1.14 2.2 135b cand78_st1 hsa-asg- hsa-asg- 8.2 7.67 8.90 8.018.20 0.52 100 7.76 7.99 6.00 6.97 7.55 7.49 6.32 6.92 6.61 7.07 0.68 671.36E−02 1.13 2.2 11847 11847_st1 hsa-asg- hsa-asg- 9.9 9.59 10.5 10.010.0 0.39 100 9.74 9.60 8.04 8.99 9.65 9.73 8.44 8.70 7.46 8.93 0.83 222.77E−02 1.12 2.2 4654 4654_st1 hsa-miR- hsa-miR- 12.8 12.8 12.1 13.012.7 0.40 100 12.5 11.4 11.7 12.2 11.8 11.0 11.8 10.5 11.3 11.6 0.61 1007.08E−03 1.12 2.2 10a 10a_st1 hsa-asg- hsa-asg- 8.2 8.81 8.78 8.44 8.570.27 50 8.25 8.36 6.36 7.73 8.16 8.44 6.54 7.19 6.06 7.45 0.94 334.29E−02 1.12 2.2 3933 3933_st1 hsa-asg- hsa-asg- 8.7 9.55 9.20 8.899.10 0.35 25 9.04 8.75 7.14 8.21 8.51 8.90 7.48 7.62 6.18 7.98 0.95 04.66E−02 1.12 2.2 4903 4903_st2 hsa- hsa- 8.0 7.13 7.97 7.72 7.73 0.43100 7.27 7.72 6.43 6.17 6.74 6.77 5.66 6.66 6.07 6.61 0.63 56 8.35E−031.12 2.2 cand173 cand173_st1 hsa-asg-8872 hsa-asg-8872_st1 9.0 7.90 9.149.22 8.82 0.62 100 8.38 8.46 7.42 7.49 7.92 8.36 6.83 7.60 6.84 7.700.63 89 1.27E−02 1.12 2.2 hsa-asg- hsa-asg- 9.9 9.55 10.1 9.91 9.89 0.2675 9.44 9.64 7.85 8.54 8.88 9.58 8.12 8.76 8.20 8.78 0.66 11 8.89E−031.11 2.2 11714 11714_st2 hsa-asg- hsa-asg- 5.6 6.22 5.47 5.76 5.77 0.3325 4.79 4.58 5.01 4.80 4.62 4.13 5.38 4.21 4.40 4.66 0.39 0 4.72E−041.11 2.2 5877 5877_st1 hsa-asg- hsa-asg- 11.3 10.8 10.4 11.4 11.0 0.44100 11.0 9.91 10.1 10.3 9.67 9.11 10.1 9.49 9.59 9.93 0.57 100 5.79E−031.11 2.2 13308 13308_st2 hsa-asg- hsa-asg- 7.2 6.52 7.03 7.24 7.00 0.34100 6.01 6.44 5.04 5.83 6.25 6.60 5.55 5.74 5.74 5.91 0.48 22 1.77E−031.09 2.1 6618 6618_st2 hsa-asg- hsa-asg- 8.4 7.02 7.77 6.66 7.47 0.79 757.14 7.24 5.01 6.02 6.14 6.65 5.66 7.09 6.51 6.38 0.75 44 3.67E−02 1.092.1 9574 9574_st2 hsa-asg- hsa-asg- 10.1 9.48 10.5 10.1 10.0 0.45 1009.75 9.83 8.00 9.41 9.73 9.65 8.36 8.68 7.56 9.00 0.86 100 4.06E−02 1.082.1 11112 11112_st2 hsa-asg-809 hsa-asg-809_st2 7.6 6.62 7.57 7.22 7.260.46 100 6.75 6.72 5.67 6.74 6.65 6.63 5.24 6.17 5.05 6.18 0.69 561.64E−02 1.08 2.1 hsa-asg- hsa-asg- 6.4 6.78 5.89 6.10 6.31 0.39 1005.71 5.33 5.08 5.34 6.46 4.94 4.53 4.89 4.83 5.23 0.57 22 6.20E−03 1.082.1 2967 2967_st2 hsa-asg-6068 hsa-asg- 7.0 6.79 6.68 6.96 6.86 0.16 1006.32 6.37 5.46 5.77 6.61 6.52 4.74 5.45 4.83 5.79 0.71 33 1.40E−02 1.082.1 6068_st2 hsa-asg- hsa-asg- 8.2 7.85 8.33 8.44 8.21 0.26 100 7.688.46 5.87 7.35 7.35 7.88 6.66 6.21 6.72 7.13 0.83 67 3.06E−02 1.07 2.13230 3230_st1 hsa-asg- hsa-asg- 10.1 8.30 10.1 9.73 9.58 0.87 100 9.259.17 7.88 8.10 8.93 8.83 7.75 8.24 8.43 8.51 0.56 100 2.06E−02 1.07 2.15032 5032_st2 hsa-miR-450 hsa-asg- 5.0 5.42 3.48 4.03 4.50 0.90 25 4.142.83 3.07 3.60 3.17 3.70 3.52 3.53 3.35 3.43 0.39 0 1.02E−02 1.07 2.114228_st2 hsa-asg-960 hsa-asg-960_st1 9.3 9.25 9.78 9.19 9.39 0.27 1008.88 9.40 6.85 8.60 8.90 9.05 7.65 7.96 7.66 8.33 0.84 100 3.31E−02 1.062.1 hsa- hsa- 3.9 6.47 4.15 5.73 5.07 1.23 25 4.41 4.16 4.23 4.46 4.283.30 4.45 3.08 3.81 4.02 0.51 0 4.51E−02 1.05 2.1 cand581 cand581_st1hsa-asg- hsa-asg- 10.1 8.96 10.3 9.98 9.84 0.61 100 8.99 9.90 8.06 8.779.12 9.87 7.78 8.47 8.23 8.80 0.75 100 3.30E−02 1.05 2.1 2615 2615_st2hsa-asg- hsa-asg- 9.0 8.87 9.20 9.24 9.10 0.17 100 9.01 8.97 7.13 8.038.83 8.64 7.35 7.35 7.19 8.05 0.81 100 3.05E−02 1.04 2.1 6948 6948_st2hsa-asg- hsa-asg- 11.4 11.0 10.4 11.4 11.1 0.47 100 11.1 9.92 10.2 10.69.89 9.29 10.1 9.60 9.63 10.0 0.57 100 8.58E−03 1.04 2.1 5304 5304_st1hsa-miR- hsa-miR-30e- 11.3 11.7 9.97 11.3 11.1 0.79 100 10.9 10.0 10.211.0 10.3 9.70 9.84 8.67 9.98 10.0 0.69 100 3.57E−02 1.04 2.1 30e-5p5p_st2 hsa-asg- hsa-asg- 10.9 10.5 11.5 10.8 10.9 0.44 100 10.6 10.79.04 9.81 10.6 10.6 9.28 10.0 8.64 9.94 0.80 100 3.53E−02 1.03 2.0 29062906_st2 hsa-asg- hsa-asg- 12.1 12.4 12.7 12.0 12.3 0.30 100 11.8 11.810.6 11.3 12.0 11.7 11.0 11.3 10.0 11.3 0.66 100 1.40E−02 1.03 2.0 71757175_st1 hsa-asg- hsa-asg- 10.6 9.37 10.3 9.56 9.98 0.61 100 9.08 9.858.18 9.01 9.67 9.31 8.33 8.87 8.33 8.96 0.60 100 1.62E−02 1.02 2.0 82548254_st2 hsa-asg- hsa-asg- 7.3 6.72 7.03 6.06 6.79 0.55 50 6.32 6.874.80 5.13 5.93 6.49 5.34 4.92 6.10 5.77 0.74 0 3.27E−02 1.02 2.0 72287228_st1 hsa-asg- hsa-asg- 5.0 5.77 4.46 6.07 5.34 0.72 50 5.04 4.504.23 5.19 3.57 3.82 3.99 4.39 4.19 4.32 0.53 0 1.49E−02 1.02 2.0 1307413074_st2 hsa-asg-8991 hsa-asg-8991_st2 7.7 7.72 8.65 7.29 7.86 0.57 1007.48 8.02 6.34 6.02 7.26 7.53 6.40 6.96 5.52 6.84 0.81 67 4.63E−02 1.022.0 hsa-asg-13661 hsa-asg- 6.8 6.59 5.87 7.07 6.59 0.52 75 6.17 5.973.89 5.45 6.00 5.73 5.38 5.84 5.78 5.58 0.68 0 2.37E−02 1.01 2.013661_st1 hsa-asg-1133 hsa-asg- 10.0 9.31 10.1 9.77 9.81 0.37 25 9.309.27 7.85 8.65 9.39 9.31 8.47 8.65 8.33 8.80 0.54 11 6.53E−03 1.01 2.01133_st2 hsa-asg-12941 hsa-asg- 9.3 10.7 10.0 8.85 9.75 0.83 100 9.509.03 7.54 8.99 9.12 8.36 8.66 9.53 7.94 8.74 0.68 100 4.16E−02 1.00 2.012941_st1 SD, standard deviation; %, percentage of samples expressingmiRNA above background levels; Log2Diff, difference in VSN-transformedexpression between NCo and LNneg samples.

A comparison of results from the two different microarray platformsrevealed that most of the miRNAs identified as differentially expressedwith the mirVana Bioarray platform were among the differentiallyexpressed miRNAs identified on the miRChip microarray platform andinclude hsa-miR-200b, -200a, -200c, -141, -215, -375, -192, -194, -429,-203, -187, 133a, and -9 (Table 16 and Table 17 above). Fold changevalues for these microRNAs differed between the two platforms,reflecting differences in the probes, samples, and data analysis tools.In contrast, seven miRNAs (hsa-miR-1, -137, -10a, -422a, -124a, and-422b and ambi-miR-7072-corresponding to hsa-asg-13254 on the miRChip),identified on the mirVana Bioarray platform as significantlyunder-expressed in LNneg samples (Table 16), were not found to besignificantly differentially expressed on the miRChip microarrayplatform. This is because the fold changes observed for these miRNAs onthe miRChip platform were less than the 5-fold cut-off used for thosedata. The inventors identified three additional miRNAs (hsa-miR-605,-200a* and -133b) that were reported as significantly differentiallyexpressed on the miRChip platform for which no probes were present onthe mirVana Bioarray platform.

Example 17 QRT-PCR Verification of Differentially Expressed Micro-RNAsin Lymph Nodes from Colon Cancer Patients

The inventors evaluated the differential expression of selected miRNAsin seven cancer-positive lymph nodes from colon cancer patients (LNpos)and in seven control cancer-negative lymph nodes (LNneg, Example 15) byqRT-PCR. The LNpos samples were from patients withhistologically-identified stage III colon cancer (T3N1M0 or T3N1M1-Table15). qRT-PCR reactions were performed using TaqMan® MicroRNA Assays(Applied Biosystems; Foster City, Calif., USA) specific for fifteen ofthe most differentially expressed miRNAs between normal colon (NCo) andcancer-negative lymph node (LNneg) samples (hsa-miR-1, -9, -141, -187,-192, -194, -200a, -200b, -200c, -203, -215, -375, -429, -566, and-605). Assays were performed according to the manufacturer'sinstructions with 15 ng of input total RNA from one of the fourteensamples and were incubated in a 7900HT Fast Real-Time PCR system(Applied Biosystems, Foster City, Calif., USA). Initial data analysiswas done using the 7500 Fast System SDS V2.3 software (AppliedBiosystems). miR-16, whose expression level did not change between thedifferent samples, was used for normalization. Equivalent t-tests wereperformed on the C_(t) values for individual qRT-PCR reaction withoutcorrection for multiple testing.

Significant differential expression between cancer-positive andcancer-negative lymph nodes was demonstrated by qRT-PCR quantificationfor ten of the fifteen miRNAs (miR-429, -200b, -141, -200a, -200c, -375,-203, -194, -192, -and -215) with fold changes varying between 760 fold(miR-429) and 77 fold (miR-215) (FIG. 10).

These data demonstrate that qRT-PCR quantification of selected miRNAs isan effective method for quantifying miRNA expression when evaluatingmetastasis of colon cancer to lymph nodes.

Example 18 Identification of Classifiers for Cancer-Positive Lymph Nodesin Patients with Metastatic Colon Cancer

The inventors have demonstrated that miRNA quantification and expressionanalysis can differentiate cancer-positive lymph nodes from patientswith colon cancer and control cancer-negative lymph nodes. To furtherdemonstrate the utility of specific miRNA markers as diagnosticanalytes, the inventors quantified specific combinations of miRNAs byqRT-PCR to determine if any such combinations enhanced the distinctionbetween cancer-positive and cancer-negative lymph nodes. qRT-PCR assayswere performed as described in Example 17 above. For miRNA combinationsquantified by qRT-PCR, normalized Ct values (miRNACt-miR-16Ct) from thequantification of individual miRNAs were added. The inventors determinedthat quantification of several miRNA combinations (miR-200b+miR-429;miR-200b+miR-203; miR-203+miR-429; miR-200b+miR-203+miR-429;miR-200a+miR-203+miR-429) enhances the distinction betweencancer-positive and cancer-negative lymph nodes (FIG. 11).

These combinations serve as non-limiting examples. Combinations ofqRT-PCR or microarray data for other microRNAs will serve to enhance thedistinction between cancer-positive and cancer-negative lymph nodes.Interestingly, some miRNA combinations, such as miR-203+miR-429, canalso be used as a classifier for the identification of metastatic lymphnodes in cervical cancer patients, in breast cancer patients, or in asubset of melanoma patients, underscoring the potential broad utility ofthese markers in cancer staging.

Example 19 miRNAs Differentally Expressed Between Normal Breast TissueAnd Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous breast cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal breasttissue than in cancer-negative lymph nodes.

Total RNA, purified from three separate normal human breast tissuesamples (FirstChoice® Human Breast Total RNA, cat. no. AM6952; Ambion,Austin, Tex., USA) and from nine normal human lymph node samples(FirstChoice® Human Lymph Node Total RNA, cat. no. AM7894; Ambion,Austin, Tex., USA) was purchased from Ambion. miRNA purification andlabeling and microarray expression analyses were performed as describedabove in Example 2.

The inventors identified 872 miRNAs (87 verified, 785 predicted) withsignificant differential expression between normal breast tissues (NBR)and normal lymph nodes (LNneg) (log 2Diff breast vs LNneg≧1 andp-value<0.05) (Table 18). The average expression level of hsa-miR-205was 196-fold higher in breast tissues compared with its averageexpression level in the normal lymph node samples. Among the verifiedmiRNAs, thirteen miRNAs (hsa-miR-429, -200b, -411, -200c, -517a, -200a,-432, -368, -551b, -539, -452*, -376a, and -487b) had average expressionlevels that were 10- to 30-fold higher in breast tissue samples, and 26miRNAs had average expression levels that were 5- to 10-fold higher inbreast tissue samples. Among the predicted miRNAs, seven(hsa-asg-4209-st2, -3983_st2, -14160_st1, -13922_st1, -4803_st2,-5021_st1, and miR154-st1) had average expression levels that werebetween 10- and 20-fold higher in breast tissues, and 76 miRNAs hadaverage expression levels that were between 5- and 10-fold higher inbreast tissues.

TABLE 18 MicroRNAs Differentially Expressed Between Normal BreastSamples (NBR) and Cancer-Negative Lymph Node Samples (LNneg) fromPatients. NBR LNneg MicroRNA Probe ID 1 2 3 Mean SD % 1 2 3 4 5 6 7 8 9Mean SD % ttest log2Diff Fold hsa-miR- hsa-miR- 12.7 11.7 12.5 12.360.51 100 2.93 5.91 5.64 2.03 3.59 6.11 3.40 5.32 7.76 4.74 1.85 334.4E−05 7.62 196.2 205 205_st1 hsa-miR- hsa-miR- 8.95 6.33 7.80 7.701.31 100 3.45 1.54 3.67 2.13 2.37 2.69 2.32 2.45 5.39 2.89 1.14 01.1E−04 4.81 28.0 429 429_st1 hsa-miR- hsa-miR- 12.9 11.2 12.2 12.140.87 100 7.62 7.80 8.90 6.69 6.82 6.87 6.51 7.65 10.4 7.70 1.26 1002.3E−04 4.44 21.7 200b 200b_st1 hsa-asg- hsa-asg- 8.25 8.96 7.97 8.390.51 100 4.24 5.01 3.60 4.30 5.45 5.18 2.92 3.13 4.43 4.25 0.89 02.0E−05 4.14 17.6 4209 4209_st2 hsa-asg- hsa-asg- 9.74 10.5 9.54 9.930.52 100 4.52 6.72 5.37 5.40 6.05 7.03 5.00 5.78 6.33 5.80 0.81 01.0E−05 4.13 17.6 3983 3983_st1 hsa-miR- hsa-miR- 8.66 6.90 7.83 7.800.88 100 4.85 3.74 4.54 5.23 5.56 2.99 3.40 2.27 2.10 3.85 1.27 115.9E−04 3.95 15.4 411 411_st1 hsa-miR- hsa-miR- 13.5 12.1 13.2 12.960.74 100 9.60 8.84 10.3 8.18 8.15 8.14 7.98 8.89 11.5 9.07 1.21 1004.1E−04 3.90 14.9 200c 200c_st1 hsa-asg- hsa-asg- 6.23 6.89 6.36 6.490.35 67 0.14 3.58 3.73 2.85 0.86 3.99 3.05 1.78 3.59 2.62 1.38 0 8.6E−043.87 14.6 14160 14160_st1 hsa-miR- hsa-miR- 8.48 7.40 7.84 7.91 0.55 1004.85 5.37 6.26 3.57 3.10 3.82 4.20 3.74 1.86 4.09 1.29 0 6.6E−04 3.8214.1 517a 517a_st1 hsa-miR- hsa-miR- 8.53 7.49 8.00 8.01 0.52 100 4.995.61 6.51 3.87 3.22 3.82 4.07 3.91 2.54 4.28 1.22 22 5.3E−04 3.72 13.2517a 517b_st1 hsa-miR- hsa-miR- 10.8 7.65 9.22 9.25 1.62 100 6.27 5.086.49 5.72 5.29 4.60 4.07 4.78 7.78 5.56 1.14 67 1.2E−03 3.69 12.9 200a200a_st2 hsa-asg- hsa-asg- 5.67 8.35 8.61 7.54 1.63 100 4.71 5.40 1.664.84 5.58 5.17 4.07 2.27 1.38 3.90 1.67 0 8.1E−03 3.64 12.5 1392213922_st1 hsa-miR- hsa-miR- 9.43 7.10 8.81 8.45 1.20 100 4.55 5.43 5.925.53 5.77 5.68 3.50 4.28 2.74 4.82 1.13 22 7.7E−04 3.62 12.3 432 432_st2hsa-asg- hsa-asg- 7.09 7.64 7.42 7.38 0.28 100 4.94 6.05 1.79 3.52 5.425.03 3.17 1.98 2.21 3.79 1.62 0 4.0E−03 3.59 12.1 4803 4803_st2 hsa-miR-hsa-miR- 10.3 9.19 9.98 9.84 0.58 100 6.49 6.52 7.70 8.08 7.70 5.24 5.643.99 5.09 6.27 1.39 67 1.8E−03 3.56 11.8 368 368_st2 hsa-miR- hsa-miR-6.96 7.94 8.58 7.83 0.82 100 4.81 4.86 4.87 4.76 4.00 2.69 4.14 2.934.40 4.16 0.83 0 5.8E−05 3.66 12.7 551b 551b_st1 hsa-miR- hsa- 7.19 7.928.52 7.88 0.66 100 5.25 4.43 3.79 5.85 3.39 4.03 4.82 3.54 4.32 4.380.81 22 5.4E−05 3.50 11.3 551b miR167_st2 hsa-asg- hsa-asg- 8.99 6.938.44 8.12 1.07 100 5.18 4.43 6.99 6.06 5.91 3.54 4.59 3.07 2.10 4.651.57 22 5.6E−03 3.47 11.1 5021 5021_st1 hsa-miR- hsa-miR- 8.41 6.96 8.147.84 0.77 100 5.14 4.08 5.49 5.67 4.85 4.03 2.48 3.83 3.77 4.37 1.01 03.1E−04 3.47 11.0 376a 376a_st2 hsa-miR- hsa-asg- 7.52 5.89 7.62 7.010.97 100 3.33 4.30 3.39 4.00 4.85 3.69 2.92 4.07 1.86 3.60 0.87 01.8E−04 3.41 10.6 376a 14234_st1 hsa-miR- hsa-asg- 8.79 7.11 8.65 8.180.93 100 5.03 4.91 6.09 5.56 5.35 4.10 3.85 4.14 4.67 4.85 0.74 118.1E−05 3.33 10.0 376a 10049_st1 hsa-miR- hsa-miR- 6.72 5.06 6.20 5.990.85 100 2.05 3.16 2.66 3.40 2.69 2.99 1.97 2.62 1.38 2.55 0.64 02.0E−05 3.45 10.9 539 539_st1 hsa-miR- hsa-miR- 6.78 7.22 7.59 7.20 0.40100 4.76 4.66 3.39 5.42 4.97 2.92 2.32 2.27 3.39 3.79 1.19 0 7.9E−043.41 10.6 452* 452-_st1 hsa- hsa- 8.50 8.75 9.25 8.83 0.38 100 6.80 6.004.98 7.76 6.35 4.31 6.07 3.07 4.16 5.50 1.48 56 3.7E−03 3.33 10.1 miR154miR154_st1 hsa-miR- hsa-miR- 9.83 7.58 9.39 8.93 1.19 100 6.06 5.84 6.976.90 6.25 5.40 4.90 3.74 4.40 5.61 1.10 67 1.2E−03 3.33 10.0 487b487b_st2 hsa- hsa- 5.99 7.90 7.79 7.23 1.07 67 6.04 4.27 2.05 4.84 5.975.09 1.08 3.38 3.01 3.97 1.72 11 1.2E−02 3.26 9.6 cand733 cand733_st1hsa-asg- hsa-asg- 9.10 9.15 9.58 9.28 0.26 100 7.66 6.59 4.47 8.11 7.314.95 6.96 3.91 4.32 6.03 1.61 56 7.2E−03 3.24 9.5 13097 13097_st1hsa-asg- hsa-asg- 9.06 7.01 8.31 8.13 1.04 100 5.60 5.37 6.65 5.89 5.904.03 4.73 2.45 3.39 4.89 1.36 56 3.9E−03 3.24 9.4 13254 13254_st1hsa-miR- hsa-miR- 9.73 9.68 10.4 9.94 0.41 100 7.92 6.71 6.74 8.78 7.675.74 7.42 4.41 5.39 6.75 1.38 67 3.2E−03 3.19 9.1 452 452_st1 hsa-asg-hsa-asg- 10.2 9.90 9.71 9.97 0.29 100 5.80 9.48 5.28 6.28 6.63 9.24 5.335.95 7.45 6.83 1.58 33 7.8E−03 3.14 8.8 10154 10154_st1 hsa-asg-hsa-asg- 9.18 10.2 9.12 9.51 0.63 100 6.02 7.47 5.43 6.29 6.40 7.09 5.616.39 6.67 6.38 0.65 67 2.7E−05 3.14 8.8 5728 5728_st2 hsa-miR- hsa- 6.264.33 5.43 5.34 0.97 67 2.61 2.54 1.39 2.42 1.83 1.88 3.50 1.68 2.10 2.220.63 0 6.1E−05 3.12 8.7 539 miR177_st1 hsa-asg- hsa-asg- 5.95 3.91 5.465.10 1.07 67 1.65 2.16 1.92 2.03 2.90 1.78 3.59 0.93 0.93 1.99 0.85 04.1E−04 3.12 8.7 7472 7472_st2 hsa-asg-20 hsa-asg- 4.32 6.75 6.61 5.891.36 67 3.93 3.96 1.02 3.52 3.85 3.54 1.42 2.36 1.50 2.79 1.21 0 3.8E−033.10 8.6 20_st1 hsa-asg- hsa-asg- 8.26 10.5 10.5 9.78 1.32 67 8.10 7.675.28 7.68 8.03 7.00 6.23 6.39 3.77 6.68 1.44 0 8.3E−03 3.09 8.5 59965996_st2 hsa-miR- hsa-miR- 8.32 10.7 10.7 9.92 1.39 33 8.21 7.70 3.917.79 8.20 6.93 6.29 7.20 5.39 6.84 1.44 0 8.9E−03 3.08 8.4 572 572_st1hsa-miR- hsa-miR- 7.53 5.49 6.68 6.57 1.02 100 3.76 2.54 4.30 4.36 4.913.13 3.17 1.29 3.97 3.49 1.10 0 1.7E−03 3.07 8.4 154 154_st2 hsa-asg-hsa-asg- 8.20 5.39 7.04 6.87 1.41 67 3.66 2.54 4.47 3.46 3.93 4.03 3.293.64 5.27 3.81 0.76 0 5.8E−04 3.07 8.4 7637 7637_st2 hsa-asg- hsa-asg-6.38 5.28 5.86 5.84 0.55 100 3.76 3.29 3.91 3.63 3.44 2.69 1.42 1.481.62 2.80 1.03 0 7.5E−04 3.04 8.2 5617 5617_st1 hsa-asg- hsa-asg- 9.109.19 9.47 9.25 0.19 100 7.62 6.37 4.47 7.82 7.31 5.81 7.02 5.04 4.546.22 1.32 56 3.1E−03 3.03 8.2 14134 14134_st1 hsa-asg- hsa-asg- 7.815.94 7.19 6.98 0.96 100 4.49 3.42 4.87 4.95 4.11 4.18 3.59 2.08 4.073.97 0.88 0 4.9E−04 3.01 8.0 13166 13166_st2 hsa-asg- hsa-asg- 6.94 6.897.17 7.00 0.15 100 3.45 5.54 3.14 3.28 3.77 5.15 3.59 3.69 4.57 4.020.86 0 1.7E−04 2.98 7.9 7721 7721_st1 hsa-miR- hsa-miR- 6.48 4.35 5.715.51 1.08 67 2.43 2.36 1.39 3.73 2.53 2.85 3.17 1.88 2.54 2.54 0.68 01.8E−04 2.97 7.8 410 410_st1 hsa-miR- hsa-miR- 8.71 5.39 7.36 7.15 1.67100 5.07 3.83 4.93 4.43 4.39 2.85 3.85 3.83 4.54 4.19 0.68 0 9.5E−042.96 7.8 141 141_st1 hsa-asg- hsa-asg- 7.52 7.41 6.99 7.31 0.28 100 3.336.46 3.79 4.12 4.53 6.95 1.08 4.63 4.60 4.39 1.71 22 1.7E−02 2.92 7.613403 13403_st2 hsa-miR- hsa-miR- 7.06 5.00 5.96 6.01 1.03 100 2.78 2.364.21 3.40 4.21 2.99 3.50 1.29 3.25 3.11 0.91 0 9.2E−04 2.90 7.5 381381_st2 hsa-asg- hsa-asg- 8.82 9.20 8.58 8.87 0.32 100 6.77 7.23 5.176.11 7.01 6.56 5.02 5.58 4.70 6.01 0.93 56 4.9E−04 2.86 7.2 66236623_st2 hsa-asg- hsa-asg- 6.96 7.02 6.54 6.84 0.26 100 2.78 7.06 0.793.28 3.28 6.80 3.40 3.49 5.02 3.99 1.99 22 3.7E−02 2.85 7.2 87118711_st1 hsa-asg- hsa-asg- 4.52 5.92 5.55 5.33 0.73 67 2.25 3.29 2.181.72 2.76 3.13 1.79 2.62 2.74 2.50 0.55 0 3.0E−05 2.83 7.1 5176 5176_st2hsa-asg- hsa-asg- 8.89 9.23 8.76 8.96 0.24 100 6.85 7.21 5.37 6.52 6.927.10 5.19 4.96 5.09 6.13 0.96 56 6.0E−04 2.83 7.1 1375 1375_st1 hsa-hsa-cand926_st2 6.52 7.33 6.12 6.66 0.61 33 4.31 4.27 2.96 4.23 4.003.82 2.48 4.55 3.92 3.84 0.68 0 8.5E−05 2.82 7.1 cand926 hsa-asg-hsa-asg- 8.70 10.2 10.6 9.84 1.01 67 8.03 7.79 5.04 7.50 8.30 6.93 6.697.60 5.39 7.03 1.15 44 3.6E−03 2.82 7.0 5205 5205_st1 hsa-asg- hsa-asg-7.26 6.95 6.62 6.94 0.32 100 2.78 6.91 3.91 4.46 4.39 7.62 1.79 3.322.10 4.14 2.01 22 4.1E−02 2.80 7.0 11424 11424_st1 hsa-miR- hsa-miR-10.0 8.33 9.51 9.31 0.90 100 6.81 5.57 7.95 7.53 7.69 5.72 6.59 5.495.44 6.53 1.02 56 1.8E−03 2.78 6.9 127 127_st1 hsa-miR- hsa-miR- 6.474.79 4.71 5.33 0.99 33 2.61 2.16 3.06 0.75 1.74 2.17 2.92 3.07 4.47 2.551.03 0 2.2E−03 2.78 6.9 605 605_st1 hsa-asg- hsa-asg- 6.54 6.89 6.876.77 0.20 100 4.17 5.30 3.79 4.00 4.97 4.75 1.60 2.93 4.47 4.00 1.14 112.2E−03 2.77 6.8 12947 12947_st1 hsa-asg- hsa-asg- 6.70 6.90 6.51 6.700.20 100 5.48 5.17 2.54 1.93 4.08 5.35 2.32 4.66 3.92 3.94 1.37 07.0E−03 2.77 6.8 13417 13417_st2 hsa-miR- hsa-miR- 10.2 8.47 9.34 9.360.89 100 6.52 6.42 7.11 6.97 7.04 6.59 6.35 6.14 6.23 6.60 0.36 1001.0E−05 2.76 6.8 379 379_st1 hsa-asg- hsa-asg- 6.98 5.17 6.34 6.16 0.9267 4.66 4.30 3.39 4.46 2.69 2.77 2.15 2.78 3.53 3.41 0.89 0 9.9E−04 2.756.7 924 924_st1 hsa-miR- hsa-miR- 8.36 6.67 7.44 7.49 0.85 100 5.73 4.495.80 6.38 5.99 2.99 5.05 2.45 3.97 4.76 1.39 44 1.0E−02 2.73 6.6 299-5p299-5p_st2 hsa-asg- hsa-asg- 10.1 10.1 9.64 10.00 0.31 100 8.49 8.906.16 7.50 7.67 8.35 5.87 6.05 6.44 7.27 1.17 56 3.0E−03 2.73 6.6 16991699_st2 hsa-asg- hsa-asg- 11.0 10.7 10.3 10.69 0.32 100 7.27 10.1 7.207.56 7.99 9.98 6.73 7.46 7.47 7.97 1.22 100 4.0E−03 2.71 6.6 1272912729_st1 hsa-asg- hsa-asg- 7.15 9.60 10.0 8.94 1.57 67 6.93 7.30 4.986.81 8.07 7.15 6.29 4.66 3.87 6.23 1.41 0 1.7E−02 2.71 6.6 9510 9510_st1hsa-asg- hsa-asg- 6.77 6.55 6.20 6.51 0.29 100 2.43 5.72 3.23 2.85 3.395.09 3.05 4.14 4.40 3.81 1.10 11 2.1E−03 2.70 6.5 8955 8955_st2 hsa-miR-hsa-miR- 8.68 6.95 8.00 7.88 0.87 100 5.11 5.78 5.99 5.85 5.70 4.21 4.734.31 5.02 5.19 0.68 0 2.2E−04 2.69 6.4 134 134_st2 hsa-asg- hsa-asg-8.49 8.85 8.62 8.65 0.18 100 6.77 7.55 5.09 5.80 6.85 6.60 5.09 5.194.78 5.97 0.99 56 1.1E−03 2.69 6.4 12996 12996_st1 hsa- hsa-cand503_st27.39 7.80 6.84 7.34 0.48 100 5.80 6.64 4.17 3.57 5.19 6.36 1.60 4.254.43 4.67 1.56 22 1.7E−02 2.68 6.4 cand503 hsa-asg- hsa-asg- 4.42 7.757.90 6.69 1.97 67 4.76 4.49 3.23 4.30 5.75 4.55 3.77 3.91 1.38 4.01 1.2111 1.6E−02 2.68 6.4 3081 3081_st1 hsa-asg- hsa-asg- 9.70 9.43 9.08 9.400.31 100 6.57 8.76 5.60 6.57 7.38 8.88 5.49 5.90 5.42 6.73 1.34 567.8E−03 2.67 6.4 14137 14137_st1 hsa-miR- hsa-miR- 11.9 11.4 11.6 11.690.25 100 9.40 9.08 9.04 10.6 10.3 8.25 9.05 6.55 8.85 9.03 1.19 1003.9E−03 2.67 6.3 199b 199b_st2 hsa-miR- hsa-asg- 6.15 4.90 5.55 5.530.62 67 2.43 3.02 3.91 2.77 2.53 2.07 2.32 3.64 3.09 2.87 0.61 0 6.8E−052.66 6.3 516-5p 13160_st1 hsa-asg- hsa-asg- 5.51 6.82 6.15 6.16 0.66 333.07 5.71 2.31 2.69 2.28 6.12 2.32 2.36 4.70 3.51 1.57 0 1.9E−02 2.666.3 6008 6008_st2 hsa-miR- hsa-miR- 6.49 7.34 6.93 6.92 0.43 100 4.714.46 4.84 6.39 5.46 2.17 6.16 3.59 0.63 4.27 1.88 22 4.0E−02 2.65 6.3378 378_st1 hsa-miR- hsa-miR- 8.97 6.65 8.08 7.90 1.17 100 6.02 4.816.97 6.26 6.32 4.43 5.16 2.45 4.89 5.26 1.35 56 1.2E−02 2.65 6.3 495495_st1 hsa-asg- hsa-asg- 7.79 8.92 9.04 8.58 0.69 100 6.40 7.41 3.466.18 7.07 6.93 5.92 5.20 4.92 5.94 1.24 67 6.4E−03 2.64 6.2 10201020_st2 hsa-miR- hsa-miR- 7.11 4.89 6.27 6.09 1.12 100 3.56 2.71 4.474.36 4.36 2.53 2.92 2.62 3.53 3.45 0.80 0 1.0E−03 2.64 6.2 369-5p369-5p_st2 hsa-miR- hsa-asg- 6.67 5.10 6.26 6.01 0.82 67 3.76 3.42 4.022.23 3.72 3.73 3.40 2.78 3.53 3.40 0.56 0 8.5E−05 2.61 6.1 369-5p1521_st1 hsa-miR- hsa-miR- 6.43 4.84 5.52 5.60 0.80 67 4.55 3.74 3.911.21 2.53 2.61 2.78 2.62 2.74 2.97 0.98 0 1.8E−03 2.63 6.2 516-5p516-5p_st1 hsa-asg- hsa-asg- 8.43 8.85 8.74 8.67 0.22 100 6.77 7.40 4.966.31 6.94 6.77 5.51 4.89 4.81 6.04 1.00 56 1.4E−03 2.63 6.2 41164116_st2 hsa-asg- hsa-asg- 8.16 7.87 7.70 7.91 0.24 100 4.37 7.42 4.124.84 4.85 7.60 2.78 5.19 6.37 5.28 1.58 33 1.9E−02 2.63 6.2 1377013770_st1 hsa-asg- hsa-asg- 6.04 8.11 8.07 7.41 1.19 100 5.98 5.12 3.235.27 6.03 5.05 4.54 3.91 3.97 4.79 0.95 44 2.9E−03 2.62 6.1 1307713077_st1 hsa-asg- hsa-asg- 9.22 9.36 9.13 9.24 0.11 100 7.34 7.90 5.306.60 7.15 7.33 5.35 6.34 6.26 6.62 0.90 78 6.6E−04 2.62 6.1 555 555_st1hsa-asg- hsa-asg- 10.9 10.5 10.5 10.65 0.22 100 7.09 10.3 7.00 7.93 8.2010.6 6.70 7.21 7.18 8.04 1.48 100 1.4E−02 2.61 6.1 9112 9112_st2hsa-asg- hsa-asg- 5.69 6.65 5.60 5.98 0.58 33 3.93 4.63 1.79 1.31 5.455.35 1.25 3.49 3.17 3.37 1.64 0 2.5E−02 2.60 6.1 13498 13498_st1hsa-asg- hsa-asg- 9.35 9.41 8.91 9.22 0.27 100 6.37 8.24 5.55 6.45 7.028.27 5.73 5.96 6.10 6.63 1.02 78 1.7E−03 2.59 6.0 5998 5998_st1 hsa-miR-hsa-miR- 6.94 6.21 6.37 6.51 0.38 67 2.43 5.25 4.17 3.91 3.85 5.50 3.681.58 4.87 3.92 1.27 0 7.0E−03 2.59 6.0 654 654_st1 hsa-miR- hsa-asg-6.88 5.80 6.42 6.37 0.54 100 3.21 6.25 5.09 4.52 4.45 5.20 4.59 4.213.65 4.58 0.89 11 9.1E−03 1.79 3.5 654 33_st1 hsa-miR- hsa-miR- 10.08.52 9.34 9.29 0.75 100 6.40 7.30 7.22 6.96 6.63 7.66 5.73 5.86 6.606.71 0.65 100 1.7E−04 2.58 6.0 494 494_st1 hsa-miR- hsa- 9.28 7.90 8.558.58 0.69 100 6.83 6.55 6.85 6.43 6.38 7.10 5.40 5.79 6.89 6.47 0.55 892.9E−04 2.11 4.3 494 miR61_st2 hsa-asg- hsa-asg- 7.60 7.93 6.79 7.440.58 100 4.09 6.15 3.73 5.02 5.12 6.20 2.92 5.06 5.55 4.87 1.10 223.5E−03 2.57 5.9 5005 5005_st2 hsa-asg- hsa-asg- 6.72 5.50 5.26 5.830.78 100 3.07 2.54 4.81 2.42 2.53 2.77 3.17 3.91 4.16 3.27 0.84 09.3E−04 2.56 5.9 1296 1296_st1 hsa- hsa- 7.62 8.05 7.45 7.71 0.31 1006.49 6.58 3.46 5.75 5.33 6.38 4.27 4.25 3.82 5.15 1.22 33 5.9E−03 2.565.9 miR207 miR207_st1 hsa-asg- hsa-asg- 8.97 9.47 8.89 9.11 0.32 1007.64 7.55 5.12 7.19 7.36 7.69 5.92 5.87 4.70 6.56 1.17 67 4.6E−03 2.555.9 12172 12172_st2 hsa-miR- hsa-miR- 10.4 8.24 9.34 9.32 1.08 100 7.335.89 7.21 8.24 6.45 5.41 5.19 6.07 9.36 6.79 1.37 78 1.6E−02 2.53 5.8203 203_st2 hsa-asg- hsa-asg- 5.34 4.39 4.68 4.80 0.49 67 1.65 0.95 3.792.33 2.69 2.35 0.35 3.20 3.25 2.29 1.12 0 4.3E−03 2.52 5.7 1370513705_st1 hsa-asg- hsa-asg- 8.60 9.62 8.40 8.87 0.66 100 6.69 7.02 5.356.60 7.31 6.94 5.92 5.83 5.64 6.37 0.69 67 2.6E−04 2.51 5.7 94349434_st2 hsa-asg- hsa-asg- 9.84 9.97 9.75 9.85 0.11 100 7.77 8.39 6.757.39 8.02 8.01 6.85 6.94 5.97 7.34 0.78 0 2.9E−04 2.51 5.7 1116511165_st1 hsa- hsa-cand138_st1 7.07 6.45 6.13 6.55 0.48 100 2.93 5.643.06 3.63 3.68 6.00 3.05 3.54 5.00 4.06 1.17 22 5.8E−03 2.49 5.6 cand138hsa-asg- hsa-asg-10630_st1 6.65 6.61 6.35 6.54 0.16 100 4.49 5.40 3.233.77 4.27 5.80 3.29 3.83 2.33 4.05 1.09 0 3.3E−03 2.49 5.6 10630hsa-asg- hsa-asg- 9.89 9.78 9.62 9.76 0.13 100 7.21 8.52 6.68 7.00 7.938.11 6.22 7.10 6.83 7.29 0.74 100 2.4E−04 2.47 5.6 3545 3545_st1hsa-asg- hsa-asg- 6.58 5.94 6.64 6.39 0.39 100 5.18 4.49 4.02 1.72 3.392.85 3.85 5.08 4.67 3.92 1.12 11 4.5E−03 2.47 5.6 9980 9980_st1 hsa-asg-hsa-asg- 8.06 8.18 7.65 7.96 0.27 100 6.89 6.21 4.26 5.61 5.88 6.16 4.595.20 4.70 5.50 0.88 11 8.9E−04 2.46 5.5 9201 9201_st2 hsa-miR- hsa-miR-10.0 8.04 9.43 9.18 1.04 100 7.17 6.85 7.35 7.13 7.43 6.56 6.29 5.935.81 6.72 0.61 78 4.3E−04 2.45 5.5 382 382_st1 hsa- hsa- 9.21 10.3 10.09.85 0.58 67 7.56 8.28 6.14 7.20 7.97 8.19 6.60 7.58 7.11 7.40 0.72 03.4E−04 2.45 5.5 miR112 miR112_st1 hsa-miR- hsa-miR- 7.87 8.08 8.52 8.160.33 100 6.96 5.82 5.71 6.66 5.54 4.84 5.51 5.11 5.23 5.71 0.70 331.9E−04 2.45 5.5 215 215_st1 hsa- hsa- 5.71 4.22 5.43 5.12 0.79 67 2.251.96 3.79 3.63 2.69 2.17 3.59 1.29 2.74 2.68 0.86 0 1.4E−03 2.44 5.4cand206 cand206_st1 hsa-asg-14163 hsa-asg- 7.40 8.81 9.03 8.41 0.89 1006.59 6.24 6.60 7.29 5.69 5.73 6.30 4.91 4.40 5.97 0.90 78 2.1E−03 2.445.4 14163_st2 hsa-asg- hsa-asg- 8.37 8.69 8.32 8.46 0.20 100 6.89 6.985.06 5.95 6.49 7.01 4.94 6.34 4.57 6.03 0.95 67 1.6E−03 2.43 5.4 62776277_st2 hsa-asg- hsa-asg- 11.1 11.2 10.9 11.11 0.17 100 9.39 10.1 8.178.70 8.96 9.66 7.51 7.93 7.66 8.69 0.94 44 1.5E−03 2.42 5.4 85428542_st2 hsa-asg- hsa-asg- 7.78 7.98 7.61 7.79 0.19 100 5.45 7.03 3.965.50 5.53 7.12 3.68 4.89 5.12 5.36 1.17 22 6.1E−03 2.42 5.4 1416414164_st2 hsa-asg- hsa-asg- 8.60 9.00 8.77 8.79 0.20 100 6.96 7.73 5.266.60 7.01 7.52 5.84 6.33 4.11 6.37 1.15 78 5.6E−03 2.42 5.3 48644864_st1 hsa-asg- hsa-asg- 8.50 10.3 10.7 9.86 1.20 100 7.95 8.62 5.857.75 8.67 8.30 6.83 7.02 6.03 7.45 1.06 78 7.7E−03 2.41 5.3 1235612356_st1 hsa-asg- hsa-asg- 8.46 8.04 8.12 8.21 0.23 100 5.18 8.00 4.585.43 5.79 7.67 4.27 4.98 6.24 5.79 1.30 22 1.1E−02 2.41 5.3 27852785_st2 hsa-cand22 hsa- 7.02 8.49 8.90 8.14 0.99 100 6.14 6.20 6.436.81 5.35 5.37 5.82 5.67 3.77 5.73 0.88 78 2.4E−03 2.41 5.3 cand22_st2hsa-asg- hsa-asg- 5.34 5.35 3.99 4.89 0.78 67 3.21 2.71 1.66 3.01 1.741.58 1.97 2.45 4.07 2.49 0.84 0 1.4E−03 2.40 5.3 12016 12016_st2hsa-miR- hsa-miR- 6.07 3.79 4.93 4.93 1.14 67 2.25 2.87 2.66 3.40 2.193.26 0.91 2.27 2.92 2.53 0.75 0 1.5E−03 2.40 5.3 487a 487a_st2 hsa-miR-hsa-asg- 6.26 5.45 5.82 5.85 0.40 100 4.09 3.64 3.91 3.40 3.89 3.78 3.683.74 3.65 3.75 0.20 0 2.1E−07 2.09 4.3 487a 6915_st1 hsa-asg- hsa-asg-5.65 4.31 5.48 5.14 0.73 67 2.05 3.02 3.67 2.03 2.45 2.17 1.60 2.93 4.782.75 0.99 0 3.4E−03 2.40 5.3 3822 3822_st2 hsa-asg- hsa-asg- 10.0 10.310.1 10.16 0.16 100 8.15 9.08 6.45 8.11 8.55 8.76 6.87 7.28 6.68 7.770.97 78 2.1E−03 2.39 5.2 13083 13083_st1 hsa-asg- hsa-asg- 9.64 10.09.54 9.73 0.24 100 8.08 8.38 6.09 7.77 8.09 7.82 6.62 6.78 6.41 7.340.86 44 9.4E−04 2.39 5.2 9920 9920_st1 hsa- hsa- 5.49 4.22 5.59 5.100.77 67 3.07 1.96 2.86 3.01 2.28 2.69 2.92 2.08 3.53 2.71 0.51 0 9.4E−052.39 5.2 cand852 cand852_st2 hsa-asg- hsa-asg- 5.34 4.31 5.09 4.91 0.5467 2.43 2.71 1.92 1.93 2.01 2.53 2.92 2.62 3.65 2.53 0.55 0 6.9E−05 2.395.2 5119 5119_st1 hsa-miR- hsa-miR- 11.4 11.7 11.9 11.72 0.22 100 10.99.25 9.49 10.8 10.1 8.36 10.8 6.81 7.36 9.33 1.54 100 2.7E−02 2.38 5.2224 224_st2 hsa-miR- hsa-asg- 11.6 11.7 11.9 11.81 0.16 100 11.0 9.309.68 10.9 10.3 8.59 11.0 7.00 7.45 9.49 1.53 100 2.9E−02 2.33 5.0 22414247_st1 hsa-asg- hsa-asg- 8.33 9.04 9.24 8.87 0.48 33 7.01 7.33 4.936.26 7.34 7.13 5.91 6.51 6.00 6.49 0.81 0 7.7E−04 2.38 5.2 8293 8293_st2hsa-miR- hsa-miR- 5.42 4.66 4.75 4.94 0.41 33 2.05 2.16 4.68 2.69 2.281.97 1.60 3.07 2.54 2.56 0.90 0 1.5E−03 2.38 5.2 518f* 518f-_st2hsa-asg- hsa-asg- 10.2 10.3 9.92 10.14 0.20 100 8.55 8.88 6.92 7.90 7.998.48 6.88 7.29 6.98 7.76 0.77 100 4.4E−04 2.38 5.2 7292 7292_st1hsa-asg- hsa-asg- 5.30 6.41 5.70 5.80 0.56 33 3.45 4.58 2.31 4.84 3.103.59 2.92 2.70 3.46 3.44 0.83 0 1.0E−03 2.37 5.2 7550 7550_st1 hsa-asg-hsa-asg- 7.81 8.51 8.39 8.24 0.38 100 5.87 6.55 4.78 6.21 6.10 6.92 4.905.92 5.64 5.88 0.70 33 2.7E−04 2.36 5.1 9053 9053_st1 hsa-asg- hsa-asg-7.45 8.01 7.80 7.75 0.28 100 5.29 7.06 5.04 5.08 5.33 5.98 4.38 5.155.42 5.41 0.74 33 4.1E−04 2.34 5.1 7704 7704_st2 hsa-miR- hsa-asg- 6.644.55 5.36 5.52 1.05 67 1.65 3.16 3.79 3.73 4.42 2.35 3.68 2.78 3.09 3.180.84 0 2.7E−03 2.33 5.0 329 13589_st2 hsa-asg- hsa-asg- 6.30 6.39 5.906.20 0.26 67 4.02 5.45 2.96 3.95 4.85 5.40 1.97 2.85 3.32 3.86 1.20 09.0E−03 2.33 5.0 2226 2226_st1 hsa-asg- hsa-asg- 10.2 10.5 10.0 10.270.22 100 8.86 9.33 7.17 7.92 8.37 8.86 6.91 7.43 6.61 7.94 0.97 222.4E−03 2.33 5.0 1751 1751_st2 hsa-asg- hsa-asg- 7.43 7.99 7.44 7.620.32 100 6.34 6.79 3.91 5.35 5.58 5.65 3.77 5.11 5.23 5.30 0.99 223.0E−03 2.32 5.0 11235 11235_st2 hsa-asg- hsa-asg- 6.68 7.07 6.48 6.740.30 100 5.11 5.32 5.30 5.02 5.43 4.55 3.59 2.70 2.83 4.43 1.10 05.6E−03 2.31 5.0 14015 14015_st1 hsa-asg- hsa-asg- 5.46 5.53 5.09 5.360.24 33 1.85 4.49 2.86 2.03 2.90 4.18 1.79 3.20 4.16 3.05 1.04 0 4.1E−032.31 5.0 13665 13665_st2 hsa-asg- hsa-asg- 8.88 9.10 8.80 8.93 0.16 1007.16 8.01 5.35 6.84 6.84 7.40 6.05 5.68 6.24 6.62 0.86 67 1.1E−03 2.314.9 3230 3230_st1 hsa-miR- hsa-miR- 6.91 7.19 7.10 7.07 0.14 100 5.295.92 2.54 5.43 5.14 5.90 3.77 4.71 4.20 4.77 1.10 0 5.8E−03 2.30 4.9 557557_st1 hsa-asg- hsa-asg- 8.34 7.05 7.95 7.78 0.66 100 6.06 5.72 5.286.77 6.26 5.43 5.46 4.21 4.16 5.48 0.87 44 2.0E−03 2.30 4.9 1417614176_st1 hsa-asg- hsa-asg- 6.92 6.96 7.32 7.07 0.22 100 6.30 5.78 4.264.26 5.12 5.09 4.54 4.25 3.32 4.77 0.90 22 1.7E−03 2.30 4.9 89028902_st2 hsa-asg- hsa-asg- 8.34 8.57 8.23 8.38 0.17 100 6.12 7.47 4.935.83 6.23 7.63 4.90 5.32 6.48 6.10 0.99 67 3.2E−03 2.28 4.9 1003010030_st2 hsa-asg- hsa-asg- 5.51 3.85 5.06 4.81 0.86 67 2.78 2.16 4.021.72 2.19 2.35 0.35 3.20 3.97 2.53 1.14 0 1.0E−02 2.28 4.9 1024710247_st1 hsa-asg- hsa-asg- 6.89 7.37 7.21 7.16 0.24 100 4.37 5.71 5.214.49 5.36 5.03 3.85 4.89 5.07 4.89 0.57 0 6.1E−05 2.27 4.8 1187711877_st1 hsa-asg- hsa-asg- 9.29 9.62 9.47 9.46 0.16 67 7.76 8.47 6.197.32 8.15 8.18 6.48 6.67 5.49 7.19 1.03 0 4.2E−03 2.27 4.8 1273 1273_st2hsa-asg- hsa-asg- 6.26 6.69 6.24 6.40 0.26 100 3.33 5.08 4.02 4.40 3.284.70 3.59 3.54 5.27 4.13 0.76 0 5.8E−04 2.27 4.8 3646 3646_st1 hsa-asg-hsa-asg- 6.68 6.65 6.65 6.66 0.02 100 4.94 5.67 3.23 4.33 4.74 5.54 3.503.74 3.87 4.40 0.88 11 1.5E−03 2.27 4.8 328 328_st1 hsa-asg- hsa-asg-6.20 4.35 5.99 5.52 1.01 67 3.33 3.16 3.54 2.51 2.53 3.26 2.64 3.20 5.093.25 0.78 0 2.2E−03 2.27 4.8 10131 10131_st2 hsa-asg- hsa-asg- 10.0 10.310.0 10.15 0.17 100 8.63 9.25 6.50 8.17 8.50 8.84 7.09 7.33 6.65 7.881.01 100 3.8E−03 2.26 4.8 5189 5189_st2 hsa-asg- hsa-asg- 8.59 9.07 8.568.74 0.29 100 6.86 7.13 5.69 6.75 7.36 7.65 5.29 5.83 5.88 6.49 0.84 671.2E−03 2.25 4.8 8683 8683_st1 hsa-asg- hsa-asg- 8.40 8.87 8.32 8.530.29 100 7.02 7.61 5.01 6.55 6.98 7.28 5.43 5.43 5.25 6.29 1.00 563.9E−03 2.25 4.7 13559 13559_st2 hsa-asg- hsa-asg- 7.87 7.76 7.67 7.760.10 100 4.81 8.13 3.54 5.08 5.33 8.28 4.82 4.91 4.89 5.53 1.59 224.0E−02 2.23 4.7 3988 3988_st2 hsa-asg- hsa-asg- 6.16 6.12 5.77 6.010.21 100 2.93 5.55 2.66 3.01 3.49 6.24 2.92 3.64 3.65 3.79 1.26 221.4E−02 2.23 4.7 4199 4199_st1 hsa-asg- hsa-asg- 9.79 10.0 9.84 9.910.16 100 8.41 8.76 6.23 7.51 8.14 8.90 6.31 7.53 7.32 7.68 0.97 113.3E−03 2.23 4.7 4869 4869_st1 hsa-miR-96 hsa-miR- 6.30 4.66 5.52 5.500.82 67 4.99 3.42 3.06 3.01 4.11 2.85 3.05 1.88 3.09 3.27 0.86 112.9E−03 2.22 4.7 96_st1 hsa-asg- hsa-asg- 5.42 6.31 5.95 5.89 0.45 1001.85 5.82 1.92 3.40 2.76 5.68 3.68 3.07 4.84 3.67 1.48 11 3.2E−02 2.224.7 8792 8792_st1 hsa-asg- hsa-asg- 6.13 6.09 6.31 6.18 0.12 100 3.075.49 3.06 3.77 4.14 5.24 3.93 2.45 4.47 3.96 1.01 0 4.3E−03 2.22 4.71797 1797_st2 hsa-asg- hsa-asg- 7.41 7.64 7.35 7.47 0.15 100 5.80 6.424.26 4.60 5.42 6.01 4.73 4.38 5.61 5.25 0.78 0 7.6E−04 2.22 4.7 72287228_st1 hsa-asg- hsa-asg- 9.92 10.3 9.89 10.04 0.24 100 8.37 8.96 6.338.10 8.40 8.58 7.04 7.43 7.18 7.82 0.86 100 1.6E−03 2.22 4.6 960 960_st1hsa-asg- hsa-asg- 6.53 6.87 6.61 6.67 0.18 100 4.43 5.77 4.12 3.63 5.055.30 3.50 3.74 4.54 4.45 0.79 22 9.0E−04 2.22 4.6 6813 6813_st2 hsa-asg-hsa-asg- 8.62 9.26 8.95 8.95 0.32 100 7.09 7.60 5.43 6.98 7.50 7.39 5.866.94 5.77 6.73 0.82 78 1.2E−03 2.22 4.6 8305 8305_st2 hsa-miR- hsa-miR-5.93 4.35 4.59 4.96 0.85 33 3.07 2.87 2.18 3.91 2.61 1.78 2.15 3.20 2.922.74 0.65 0 7.3E−04 2.22 4.6 200a* 200a-_st2 hsa-miR- hsa-miR- 7.12 3.965.88 5.65 1.59 67 3.76 3.02 4.61 3.28 4.58 2.85 2.78 3.32 2.74 3.44 0.730 6.3E−03 2.21 4.6 329 329_st1 hsa-miR- hsa-asg- 6.63 4.12 5.52 5.421.26 67 4.09 2.87 4.21 3.40 4.24 3.48 4.00 2.62 3.53 3.61 0.59 0 5.2E−031.82 3.5 329 13204_st2 hsa-asg- hsa-asg- 7.79 8.09 7.48 7.78 0.31 1005.32 6.90 4.43 5.37 5.27 6.38 5.25 5.71 5.55 5.57 0.71 22 4.7E−04 2.214.6 11940 11940_st1 hsa-miR- hsa-miR- 8.85 7.01 7.91 7.92 0.92 100 5.806.16 5.53 5.92 5.78 3.48 5.82 5.72 7.23 5.72 0.97 89 6.3E−03 2.21 4.6424 424_st1 hsa-miR- hsa-miR- 7.10 4.92 5.91 5.97 1.09 67 4.24 2.71 2.863.91 3.63 4.03 4.20 4.47 4.07 3.79 0.61 0 1.2E−03 2.18 4.5 450 450_st2hsa-miR- hsa-asg- 5.90 4.12 4.68 4.90 0.91 33 3.56 2.16 2.43 3.01 2.533.13 2.92 2.93 2.74 2.82 0.41 0 2.1E−04 2.08 4.2 450 14228_st2 hsa-asg-hsa-asg- 10.3 10.4 10.0 10.30 0.24 100 8.84 9.32 7.09 8.22 8.76 9.207.09 7.63 6.86 8.11 0.97 100 3.7E−03 2.18 4.5 5810 5810_st2 hsa-asg-hsa-asg- 7.21 7.59 7.56 7.45 0.21 100 5.80 5.91 4.93 5.25 6.11 6.04 4.144.91 4.32 5.27 0.74 33 6.4E−04 2.18 4.5 6068 6068_st2 hsa-asg- hsa-asg-10.6 10.7 10.6 10.66 0.07 100 9.15 9.59 7.72 8.56 8.81 8.90 7.84 7.847.91 8.48 0.68 33 3.1E−04 2.18 4.5 8541 8541_st1 hsa-miR- hsa-miR- 6.023.85 5.12 4.99 1.09 33 2.43 2.71 4.54 2.93 4.21 2.35 1.60 1.68 2.92 2.821.00 0 9.6E−03 2.17 4.5 433 433_st2 hsa-asg- hsa-asg- 5.83 6.63 6.236.23 0.40 100 3.85 5.04 3.91 3.63 4.39 3.95 1.79 4.58 5.42 4.06 1.04 06.3E−03 2.17 4.5 6076 6076_st2 hsa-asg- hsa-asg- 9.39 9.39 9.50 9.420.06 100 8.24 8.13 6.97 7.98 6.55 7.56 6.68 6.07 7.12 7.25 0.77 1007.8E−04 2.17 4.5 13646 13646_st1 hsa-asg- hsa-asg- 9.00 9.47 8.84 9.100.33 100 7.74 7.91 5.83 7.23 7.66 7.97 5.93 6.67 5.56 6.94 0.96 334.1E−03 2.16 4.5 3933 3933_st1 hsa-asg- hsa-asg- 6.24 6.30 6.15 6.230.07 67 4.31 5.21 3.14 3.87 3.22 5.27 3.17 4.76 3.71 4.07 0.85 0 1.7E−032.16 4.5 12526 12526_st1 hsa-asg- hsa-asg- 6.36 6.56 5.77 6.23 0.41 1002.25 5.79 3.67 3.15 3.85 5.65 3.93 3.91 4.47 4.07 1.12 11 1.0E−02 2.154.4 8424 8424_st2 hsa-asg- hsa-asg- 7.60 7.99 7.49 7.69 0.26 100 5.626.43 4.43 5.75 6.04 6.70 4.33 5.58 5.02 5.54 0.82 11 1.5E−03 2.15 4.41898 1898_st1 hsa-miR- hsa-miR- 5.88 4.51 4.83 5.07 0.72 33 3.56 2.715.04 2.69 1.55 2.99 3.17 2.62 2.10 2.94 0.98 0 6.4E−03 2.14 4.4 526c526c_st1 hsa-asg- hsa-asg- 9.75 9.96 9.32 9.68 0.33 100 8.50 8.52 6.617.52 8.33 8.17 6.75 6.82 6.70 7.55 0.84 100 1.8E−03 2.13 4.4 69486948_st2 hsa-asg- hsa-asg- 6.02 6.10 5.89 6.01 0.10 100 4.37 5.91 2.183.34 4.70 5.27 1.60 3.54 3.97 3.88 1.39 11 2.8E−02 2.13 4.4 24712471_st1 hsa-asg- hsa-asg- 9.83 9.82 10.2 9.97 0.26 100 9.05 8.95 7.038.66 7.51 8.06 7.19 6.23 7.94 7.85 0.95 100 3.9E−03 2.13 4.4 1325813258_st2 hsa-asg- hsa-asg- 3.52 5.93 6.59 5.35 1.61 33 3.21 4.33 3.313.28 3.49 2.92 2.15 2.36 3.92 3.22 0.69 0 7.2E−03 2.13 4.4 3743 3743_st1hsa-asg- hsa-asg- 5.03 7.68 7.99 6.90 1.62 67 5.18 5.62 4.34 5.21 5.435.05 3.50 4.44 4.20 4.78 0.69 0 7.5E−03 2.13 4.4 3009 3009_st1 hsa-asg-hsa-asg- 7.86 8.22 8.19 8.09 0.20 100 5.18 7.62 4.17 5.50 6.04 7.46 5.515.24 6.97 5.96 1.16 33 1.2E−02 2.13 4.4 9186 9186_st1 hsa-asg- hsa-asg-6.04 4.51 5.55 5.37 0.78 67 3.33 4.08 2.86 3.01 2.90 3.26 2.78 2.78 4.163.24 0.53 0 3.0E−04 2.13 4.4 8174 8174_st1 hsa-asg- hsa-asg- 8.38 9.7210.0 9.38 0.88 100 8.27 7.65 5.88 6.64 8.43 8.11 7.07 7.28 5.93 7.250.96 78 7.0E−03 2.12 4.4 1196 1196_st1 hsa-asg- hsa-asg- 6.85 7.13 6.616.86 0.26 100 5.07 6.19 3.73 4.19 5.54 5.17 3.68 3.78 5.33 4.74 0.92 113.2E−03 2.12 4.4 8079 8079_st1 hsa-asg- hsa-asg- 9.53 9.34 9.23 9.370.15 67 6.21 9.20 6.16 7.11 6.78 8.79 5.67 7.34 7.94 7.25 1.21 111.4E−02 2.12 4.4 6957 6957_st1 hsa- hsa- 6.70 7.38 7.04 7.04 0.34 1005.68 5.81 2.96 5.17 5.87 5.80 4.38 4.11 4.50 4.92 1.00 11 5.8E−03 2.124.3 cand902 cand902_st1 hsa-asg- hsa-asg- 10.5 10.3 10.2 10.38 0.16 1006.52 10.0 6.81 7.19 8.44 10.5 6.86 8.30 9.63 8.26 1.52 100 4.1E−02 2.124.3 3383 3383_st1 hsa-asg- hsa-asg- 8.90 9.07 8.79 8.92 0.14 100 6.857.86 5.72 6.82 7.10 7.84 6.18 6.12 6.82 6.81 0.73 89 7.2E−04 2.11 4.37654 7654_st2 hsa-asg- hsa-asg- 9.32 9.63 9.38 9.45 0.17 100 8.45 8.316.17 7.81 8.24 8.24 6.49 6.84 5.48 7.34 1.11 89 9.8E−03 2.11 4.3 54235423_st2 hsa-asg- hsa-asg- 6.02 6.49 5.93 6.15 0.30 100 4.02 5.55 3.393.77 3.85 5.20 2.92 3.99 3.65 4.04 0.83 11 1.9E−03 2.11 4.3 90999099_st2 hsa-miR- hsa-miR- 6.66 5.68 6.94 6.43 0.66 100 5.83 3.53 4.214.99 4.63 3.95 4.27 3.20 4.40 4.33 0.78 11 1.9E−03 2.09 4.3 154*154-_st2 hsa-miR- hsa-miR- 5.29 4.76 4.64 4.89 0.34 100 2.25 3.16 3.063.40 1.83 2.99 3.05 2.93 2.54 2.80 0.50 0 5.6E−05 2.09 4.3 520g 520g_st1hsa-asg- hsa-asg- 10.2 10.7 10.3 10.43 0.24 100 8.94 9.48 7.27 8.69 9.309.16 7.67 7.84 6.85 8.36 0.96 100 4.9E−03 2.08 4.2 388 388_st1 hsa-asg-hsa-asg- 6.46 5.97 6.50 6.31 0.30 100 4.43 5.12 4.02 3.21 4.39 4.18 4.144.21 4.40 4.23 0.50 0 5.2E−05 2.08 4.2 1596 1596_st2 hsa- hsa- 8.55 9.439.42 9.13 0.50 67 8.30 7.44 5.89 7.66 8.25 7.44 6.15 7.29 5.09 7.06 1.1044 1.1E−02 2.07 4.2 cand710 cand710_st2 hsa-miR- hsa-miR- 7.34 7.56 7.247.38 0.16 100 5.39 6.49 4.72 4.19 4.93 6.34 3.93 5.58 6.21 5.31 0.94 334.1E−03 2.07 4.2 612 612_st1 hsa-asg- hsa-asg- 8.98 9.28 8.83 9.03 0.2367 7.58 8.15 6.21 6.89 7.41 7.58 6.25 6.77 5.78 6.96 0.78 33 1.3E−032.07 4.2 9453 9453_st2 hsa-asg- hsa-asg- 7.75 8.01 7.90 7.89 0.13 1005.68 6.79 5.60 5.65 5.88 6.62 4.94 5.88 5.31 5.82 0.58 44 1.4E−04 2.074.2 12376 12376_st2 hsa-asg- hsa-asg- 5.31 5.92 5.31 5.51 0.35 33 3.764.37 3.79 3.34 4.00 4.37 2.78 2.08 2.54 3.45 0.82 0 2.0E−03 2.07 4.25889 5889_st2 hsa-asg- hsa-asg- 9.56 10.0 9.79 9.80 0.25 100 8.31 8.966.03 7.91 8.79 8.82 6.96 7.29 6.55 7.74 1.08 11 9.5E−03 2.06 4.2 34503450_st2 hsa-asg- hsa-asg- 6.47 8.99 9.50 8.32 1.62 33 6.42 6.94 4.616.58 7.81 7.05 6.37 5.29 5.23 6.25 1.02 11 2.4E−02 2.06 4.2 95129512_st1 hsa-asg- hsa-asg- 9.46 9.77 9.37 9.53 0.21 100 8.52 8.30 6.627.71 8.01 8.42 6.87 7.09 5.69 7.47 0.96 0 5.1E−03 2.06 4.2 4903 4903_st2hsa-asg- hsa-asg- 6.44 6.60 6.00 6.35 0.31 33 3.33 5.83 4.26 2.85 3.855.32 4.14 4.66 4.43 4.30 0.92 0 4.2E−03 2.05 4.1 6416 6416_st1 hsa-asg-hsa-asg- 10.5 10.5 10.3 10.46 0.12 100 7.62 10.1 7.19 7.75 9.04 10.07.35 8.01 8.52 8.41 1.11 100 1.1E−02 2.05 4.1 11515 11515_st2 hsa-asg-hsa-asg- 5.92 6.58 6.04 6.18 0.35 100 4.17 4.23 3.06 4.36 5.66 4.70 2.483.91 4.60 4.13 0.93 11 4.5E−03 2.05 4.1 5044 5044_st1 hsa-miR- hsa-miR-5.62 3.85 5.20 4.89 0.93 67 4.61 3.42 2.86 3.15 3.63 2.53 1.60 1.29 2.542.85 1.02 0 1.2E−02 2.04 4.1 19a 19a_st1 hsa-miR- hsa-miR- 5.07 3.024.93 4.34 1.14 67 1.85 2.16 4.12 3.34 2.69 1.01 2.78 2.53 0.21 2.30 1.180 2.5E−02 2.04 4.1 376b 376b_st1 hsa-asg- hsa-asg- 4.86 5.83 6.25 5.650.71 33 2.25 4.84 2.76 3.46 3.54 5.01 1.97 3.87 4.81 3.61 1.13 0 1.6E−022.04 4.1 8987 8987_st1 hsa-asg- hsa-asg- 9.68 9.99 9.75 9.80 0.17 1008.01 8.75 6.95 7.98 8.46 8.73 7.14 7.37 6.57 7.77 0.80 0 1.7E−03 2.034.1 11993 11993_st1 hsa-asg- hsa-asg- 10.7 10.9 10.9 10.91 0.11 100 9.139.90 7.66 8.85 9.90 9.86 8.18 8.63 7.86 8.88 0.88 44 3.1E−03 2.03 4.18688 8688_st1 hsa-asg- hsa-asg- 9.67 11.1 11.5 10.78 0.98 100 9.77 9.627.44 8.84 9.90 8.86 7.99 9.27 7.11 8.76 1.03 67 1.3E−02 2.02 4.1 62926292_st2 hsa-asg- hsa-asg- 6.81 7.60 7.68 7.36 0.48 100 5.54 6.40 3.465.78 6.01 6.71 3.59 4.93 5.64 5.34 1.15 22 1.6E−02 2.02 4.1 86308630_st2 hsa-asg- hsa-asg- 8.26 8.72 8.31 8.43 0.26 100 6.80 7.30 5.656.66 6.92 7.45 5.80 5.80 5.40 6.42 0.76 67 1.4E−03 2.01 4.0 93829382_st2 hsa-asg- hsa-asg- 6.02 3.02 4.37 4.47 1.50 33 2.93 2.16 3.391.61 2.11 2.53 2.78 2.78 1.86 2.46 0.57 0 5.0E−03 2.01 4.0 7295 7295_st2hsa-asg- hsa-asg- 10.3 10.5 10.0 10.30 0.23 100 8.48 9.45 7.54 8.27 8.629.39 7.17 7.95 7.75 8.29 0.79 100 1.7E−03 2.01 4.0 2615 2615_st2hsa-asg- hsa-asg- 8.64 9.37 8.87 8.96 0.37 100 7.36 7.93 6.27 6.86 7.297.72 6.18 6.58 6.43 6.96 0.64 100 5.2E−04 2.00 4.0 7823 7823_st1hsa-asg- hsa-asg- 7.48 8.49 7.71 7.89 0.53 100 5.78 7.43 4.72 5.37 6.717.43 4.86 5.09 5.61 5.89 1.05 33 1.1E−02 2.00 4.0 247 247_st1 hsa-asg-hsa-asg- 7.24 7.61 7.50 7.45 0.19 100 6.37 6.48 4.72 6.05 5.94 6.05 4.544.60 4.36 5.46 0.87 22 3.4E−03 1.99 4.0 1153 1153_st1 hsa-asg- hsa-asg-6.42 6.88 6.77 6.69 0.24 100 5.03 6.02 3.91 3.95 5.14 5.05 4.20 4.584.47 4.71 0.68 11 6.7E−04 1.99 4.0 748 748_st1 hsa-asg- hsa-asg- 6.826.10 6.42 6.45 0.36 100 4.61 4.95 5.67 3.77 3.10 3.54 4.86 4.83 4.844.46 0.82 0 2.6E−03 1.98 4.0 14209 14209_st1 hsa-asg- hsa-asg- 8.72 9.498.65 8.96 0.47 100 6.68 7.91 5.65 6.67 7.86 7.72 6.21 6.74 7.33 6.970.78 89 2.2E−03 1.98 4.0 1736 1736_st1 hsa-asg- hsa-asg- 7.08 7.82 8.017.64 0.49 100 6.42 6.57 4.43 5.37 6.43 6.39 4.82 5.17 5.33 5.66 0.81 442.7E−03 1.98 3.9 8138 8138_st2 hsa-asg- hsa-asg- 9.79 10.1 10.0 9.990.18 100 8.57 9.37 6.81 7.99 8.62 9.08 7.41 7.91 6.38 8.02 1.01 898.4E−03 1.98 3.9 11698 11698_st2 hsa-asg- hsa-asg- 9.03 9.34 8.84 9.070.25 100 7.34 8.24 6.24 6.81 7.45 7.73 6.07 7.05 6.93 7.10 0.69 1007.9E−04 1.98 3.9 6637 6637_st1 hsa-asg- hsa-asg- 8.58 8.91 8.78 8.760.16 100 6.91 7.87 5.76 6.85 7.60 7.46 5.98 6.57 6.03 6.78 0.76 781.5E−03 1.98 3.9 11962 11962_st1 hsa-asg- hsa-asg- 7.94 7.95 7.72 7.870.13 100 5.14 7.40 4.39 6.07 6.41 7.10 4.69 5.42 6.44 5.90 1.05 561.0E−02 1.97 3.9 9485 9485_st2 hsa-asg- hsa-asg- 7.33 9.02 9.44 8.591.12 100 6.64 7.77 5.06 6.88 7.77 6.98 6.69 5.98 5.80 6.62 0.89 781.0E−02 1.97 3.9 3597 3597_st2 hsa-miR- hsa-miR- 11.4 10.5 11.6 11.250.58 100 10.2 10.0 8.85 9.82 9.37 9.55 8.99 8.57 8.13 9.28 0.70 1001.4E−03 1.97 3.9 182 182_st1 hsa-asg- hsa-asg- 8.11 8.09 7.73 7.98 0.21100 6.32 7.05 5.06 5.89 6.31 7.01 5.29 5.92 5.29 6.01 0.73 44 1.1E−031.96 3.9 14136 14136_st2 hsa-asg- hsa-asg- 7.06 7.01 7.39 7.16 0.21 1005.70 6.17 5.12 5.53 5.14 5.25 4.54 4.44 4.87 5.19 0.55 0 1.6E−04 1.963.9 3711 3711_st2 hsa-asg- hsa-asg- 7.60 7.17 6.89 7.22 0.36 100 5.186.60 4.30 4.55 4.45 6.60 4.49 5.08 6.12 5.26 0.94 44 6.4E−03 1.96 3.93138 3138_st2 hsa-asg- hsa-asg- 4.81 6.18 5.95 5.64 0.73 67 3.66 5.523.23 3.08 4.27 4.84 1.42 4.07 3.09 3.69 1.19 11 2.4E−02 1.96 3.9 1015810158_st2 hsa-asg- hsa-asg- 8.30 8.74 8.38 8.47 0.23 100 5.96 7.81 5.575.71 8.62 7.10 6.14 5.99 5.78 6.52 1.08 67 1.2E−02 1.95 3.9 94059405_st1 hsa-asg- hsa-asg- 9.51 9.78 9.61 9.63 0.14 100 8.07 8.72 6.557.79 8.35 8.44 6.98 7.31 6.92 7.68 0.77 78 1.7E−03 1.95 3.9 1255612556_st1 hsa-miR- hsa-miR- 5.49 4.59 4.16 4.75 0.68 33 2.78 3.42 4.751.93 2.28 2.85 3.05 2.27 1.86 2.80 0.90 0 6.6E−03 1.95 3.9 526a 526a_st2hsa-asg- hsa-asg- 8.90 9.11 8.54 8.85 0.29 100 7.22 7.46 6.29 6.35 7.356.78 6.35 7.35 6.95 6.90 0.48 100 6.3E−05 1.95 3.9 773 773_st1 hsa-asg-hsa-asg- 7.65 8.51 8.40 8.19 0.47 100 6.04 7.19 5.01 5.83 6.74 6.89 5.715.96 6.76 6.24 0.70 67 1.2E−03 1.95 3.9 12381 12381_st1 hsa-asg-hsa-asg- 10.9 12.1 12.3 11.80 0.78 100 10.8 10.5 8.34 10.2 10.8 10.29.35 10.0 8.07 9.85 1.04 89 1.4E−02 1.95 3.9 1341 1341_st1hsa-miR-485-5p hsa-miR- 5.86 3.96 5.33 5.05 0.98 67 3.45 3.29 3.39 3.683.28 1.97 3.50 1.88 3.53 3.11 0.68 0 3.0E−03 1.95 3.9 485-5p_st2hsa-asg- hsa-asg- 9.16 9.85 9.73 9.58 0.37 100 8.26 8.79 6.42 7.90 8.408.35 7.26 7.14 6.19 7.63 0.92 100 6.1E−03 1.95 3.9 1861 1861_st1hsa-asg- hsa-asg- 10.2 10.3 10.0 10.23 0.14 100 9.01 9.50 7.15 8.46 9.019.29 7.69 7.72 6.75 8.29 0.99 33 8.2E−03 1.95 3.9 5379 5379_st2 hsa-miR-hsa-miR- 7.44 7.99 9.02 8.15 0.80 100 7.35 6.80 6.01 7.48 6.36 5.50 5.735.42 5.23 6.21 0.84 56 5.7E−03 1.94 3.8 489 489_st2 hsa-asg- hsa-asg-6.96 7.41 7.06 7.14 0.24 100 5.35 6.54 4.17 5.37 5.75 6.05 4.59 4.494.50 5.20 0.81 11 2.6E−03 1.94 3.8 3672 3672_st1 hsa-asg- hsa-asg- 5.816.12 5.52 5.82 0.30 100 3.07 6.08 2.66 3.57 2.61 6.37 2.92 4.21 3.393.88 1.42 22 4.6E−02 1.94 3.8 11799 11799_st1 hsa-asg- hsa-asg- 10.612.4 12.3 11.84 1.04 100 10.6 10.2 8.54 10.4 11.2 10.0 9.59 10.4 8.029.90 1.03 100 1.7E−02 1.94 3.8 11850 11850_st1 hsa-asg- hsa-asg- 6.216.94 6.28 6.48 0.40 100 5.11 5.68 3.14 5.21 5.35 4.93 4.64 4.38 2.444.54 1.08 11 1.4E−02 1.94 3.8 7981 7981_st1 hsa-asg- hsa-asg- 8.45 8.688.55 8.56 0.12 100 5.96 7.79 5.72 6.81 6.53 8.06 5.66 6.17 6.94 6.630.86 78 3.7E−03 1.93 3.8 6860 6860_st1 hsa-miR- hsa- 5.44 5.37 5.55 5.450.09 100 3.93 2.54 1.92 5.43 5.30 2.53 3.17 3.20 3.65 3.52 1.21 02.3E−02 1.93 3.8 193a cand178_st1 hsa-miR- hsa-miR- 6.32 6.41 6.42 6.380.06 100 5.73 4.76 3.06 5.53 6.52 4.49 5.88 4.35 3.65 4.88 1.13 334.9E−02 1.50 2.8 193a 193a_st1 hsa-asg- hsa-asg- 8.36 8.81 8.67 8.620.23 0 7.33 7.11 5.45 7.16 6.78 6.86 6.78 6.84 5.84 6.68 0.63 33 4.7E−041.93 3.8 872 872_st2 hsa-asg- hsa-asg- 9.84 10.3 10.2 10.15 0.28 1008.57 8.98 7.30 8.52 8.94 8.85 7.61 7.94 7.23 8.22 0.71 100 1.1E−03 1.933.8 1277 1277_st1 hsa-asg- hsa-asg- 6.43 6.63 6.34 6.47 0.15 100 4.996.05 3.73 3.87 5.19 5.73 3.68 5.43 2.21 4.54 1.24 11 2.6E−02 1.92 3.89071 9071_st1 hsa-asg- hsa-asg- 10.2 10.6 10.3 10.41 0.21 100 9.24 9.397.48 8.91 9.24 9.18 7.75 8.16 7.08 8.49 0.88 100 4.6E−03 1.92 3.8 1111211112_st2 hsa-asg- hsa-asg- 7.64 8.01 8.21 7.96 0.29 100 6.69 6.76 4.586.05 6.66 7.00 5.64 5.94 5.02 6.04 0.84 56 3.5E−03 1.92 3.8 62826282_st1 hsa-asg- hsa-asg- 9.35 9.65 9.17 9.39 0.24 100 8.28 8.62 6.977.10 7.77 8.46 6.51 7.21 6.29 7.47 0.85 0 3.8E−03 1.92 3.8 5163 5163_st1hsa-asg- hsa-asg- 5.86 5.73 5.88 5.82 0.08 100 2.93 6.18 3.67 3.95 3.496.04 1.97 3.54 3.39 3.91 1.37 11 4.1E−02 1.92 3.8 12343 12343_st1hsa-asg- hsa-asg- 5.34 6.01 5.57 5.64 0.34 100 4.02 5.19 2.66 4.40 4.682.77 4.20 2.08 3.53 3.72 1.04 0 1.2E−02 1.92 3.8 5741 5741_st2 hsa-asg-hsa-asg- 7.73 7.85 7.57 7.72 0.14 100 6.51 6.68 4.26 5.77 6.19 6.33 5.495.49 5.51 5.80 0.74 22 1.5E−03 1.91 3.8 1040 1040_st2 hsa-asg- hsa-asg-7.60 8.10 8.23 7.98 0.33 100 6.40 6.88 5.58 6.19 6.24 6.63 5.51 5.785.37 6.06 0.53 56 1.7E−04 1.91 3.8 1293 1293_st2 hsa-asg- hsa-asg- 11.111.3 11.0 11.18 0.15 100 9.79 10.1 8.16 9.48 10.1 10.1 8.64 8.97 7.819.26 0.91 89 5.5E−03 1.91 3.8 2195 2195_st1 hsa-asg- hsa-asg- 8.37 8.708.46 8.51 0.17 100 7.13 7.83 5.55 6.35 7.20 7.38 5.69 6.42 5.88 6.600.82 89 2.9E−03 1.91 3.8 11521 11521_st1 hsa- hsa- 8.65 9.12 8.69 8.820.26 100 7.44 8.08 5.65 6.94 7.39 8.04 5.90 6.55 6.26 6.92 0.89 895.2E−03 1.90 3.7 cand545 cand545_st1 hsa-asg- hsa-asg- 6.28 6.72 6.726.57 0.26 100 5.11 5.61 4.47 4.66 5.94 5.17 2.48 4.87 3.77 4.67 1.04 221.2E−02 1.90 3.7 841 841_st1 hsa-asg- hsa-asg- 8.03 8.06 8.83 8.31 0.45100 7.02 6.39 7.09 7.51 6.90 6.04 6.76 5.59 4.40 6.41 0.95 67 8.7E−031.90 3.7 13205 13205_st1 hsa-asg- hsa-asg- 7.65 8.08 8.06 7.93 0.24 1006.23 6.95 5.39 5.97 6.20 6.04 6.00 6.45 5.07 6.03 0.55 78 2.1E−04 1.893.7 10971 10971_st2 hsa-asg- hsa-asg- 5.42 3.37 3.92 4.24 1.06 33 2.782.36 2.66 2.33 1.64 2.17 1.60 2.08 3.53 2.35 0.59 0 2.6E−03 1.89 3.712139 12139_st2 hsa-asg- hsa-asg- 8.14 8.20 7.79 8.04 0.22 100 6.20 7.145.21 6.43 6.28 6.58 6.00 5.49 6.13 6.16 0.57 44 2.8E−04 1.88 3.7 23142314_st1 hsa-asg- hsa-asg- 9.02 9.41 9.10 9.18 0.20 100 7.53 8.18 6.157.58 7.94 8.12 6.38 6.75 7.03 7.29 0.75 89 1.9E−03 1.88 3.7 1268712687_st1 hsa-asg- hsa-asg- 8.07 9.40 9.22 8.90 0.72 67 8.10 7.49 5.537.17 7.78 7.18 6.38 7.87 5.66 7.02 0.95 33 1.1E−02 1.88 3.7 25352535_st2 hsa-asg- hsa-asg- 7.75 8.37 8.35 8.16 0.35 100 6.25 7.44 5.766.14 6.85 6.95 5.61 6.19 5.33 6.28 0.69 78 1.2E−03 1.88 3.7 76717671_st1 hsa-asg- hsa-asg- 8.72 8.90 8.64 8.75 0.14 100 7.18 7.78 5.656.79 7.38 7.53 6.58 6.78 6.21 6.88 0.67 89 8.9E−04 1.88 3.7 47864786_st1 hsa-asg- hsa-asg- 10.5 11.9 11.9 11.50 0.83 100 10.6 10.0 8.1410.0 10.9 9.89 9.20 10.1 7.56 9.62 1.12 100 2.5E−02 1.88 3.7 43924392_st1 hsa-asg- hsa-asg- 8.67 9.26 9.02 8.98 0.30 67 7.74 8.06 6.197.36 7.86 7.50 6.17 6.72 6.39 7.11 0.75 44 2.1E−03 1.87 3.7 79547954_st1 hsa-asg- hsa-asg- 9.71 10.1 9.64 9.85 0.30 100 8.20 9.32 6.967.64 8.53 9.24 6.70 7.97 7.22 7.97 0.94 100 8.1E−03 1.87 3.7 53785378_st2 hsa-asg- hsa-asg- 9.96 10.3 10.2 10.16 0.18 100 8.45 9.31 7.118.05 8.55 9.51 7.51 8.75 7.36 8.29 0.85 100 4.2E−03 1.87 3.7 34623462_st2 hsa-asg- hsa-asg- 9.22 9.26 8.94 9.14 0.18 100 7.64 8.08 6.566.92 7.74 7.49 6.24 6.70 8.09 7.27 0.68 100 1.0E−03 1.87 3.7 25802580_st1 hsa- hsa- 8.00 8.48 8.32 8.27 0.24 100 6.74 7.25 5.49 7.08 6.937.08 5.88 5.99 5.18 6.40 0.77 78 2.5E−03 1.87 3.6 cand266 cand266_st1hsa-asg- hsa-asg- 11.3 11.6 11.6 11.57 0.16 100 10.4 10.7 8.60 10.0 10.410.4 9.06 9.51 8.05 9.71 0.95 89 8.5E−03 1.86 3.6 6234 6234_st1 hsa-asg-hsa-asg- 7.98 8.24 7.76 7.99 0.24 100 4.85 7.01 5.74 5.83 5.99 6.54 5.496.68 7.06 6.13 0.74 56 1.9E−03 1.86 3.6 1140 1140_st2 hsa-asg- hsa-asg-6.38 6.52 6.24 6.38 0.14 100 4.81 5.42 3.60 4.40 4.04 5.17 3.85 4.764.63 4.52 0.60 0 4.3E−04 1.86 3.6 3614 3614_st1 hsa-asg- hsa-asg- 8.288.64 8.77 8.56 0.25 100 6.68 7.28 6.66 6.88 7.07 7.06 6.07 6.55 6.106.71 0.42 100 3.3E−05 1.86 3.6 11973 11973_st2 hsa-asg- hsa-asg- 6.476.04 6.50 6.34 0.26 67 5.62 5.68 2.96 5.39 5.57 4.03 3.59 2.70 4.81 4.481.19 11 2.6E−02 1.85 3.6 10391 10391_st2 hsa-asg- hsa-asg- 9.23 9.669.60 9.50 0.23 100 8.03 8.83 6.56 7.51 8.02 8.49 7.09 7.31 6.95 7.640.75 100 2.1E−03 1.85 3.6 3746 3746_st1 hsa-miR- hsa- 6.47 6.52 7.136.71 0.37 100 5.94 5.23 4.02 6.58 5.46 4.24 5.64 3.07 3.53 4.86 1.19 442.7E−02 1.85 3.6 365 cand708_st1 hsa-miR- hsa-miR- 7.69 7.62 7.76 7.690.07 100 7.27 5.64 5.53 7.63 6.38 5.40 6.66 4.78 4.78 6.01 1.03 562.1E−02 1.68 3.2 365 365_st1 hsa-miR- hsa-asg- 7.39 7.44 7.85 7.56 0.25100 7.33 5.87 6.09 7.37 6.38 5.46 6.63 4.78 4.67 6.06 0.98 56 2.9E−021.50 2.8 365 13156_st1 hsa-asg- hsa-asg- 8.28 8.59 8.35 8.41 0.16 1007.24 7.55 5.47 6.46 7.05 7.02 5.71 6.39 6.13 6.56 0.71 67 1.4E−03 1.853.6 11847 11847_st1 hsa-miR- hsa-miR- 5.44 3.82 5.12 4.79 0.86 33 2.433.42 3.39 2.93 3.44 2.77 3.29 1.88 2.92 2.94 0.52 0 1.0E−03 1.85 3.6299-3p 299-3p_st2 hsa-miR- hsa-asg- 5.42 3.72 4.16 4.43 0.88 33 3.663.42 3.23 1.93 2.45 1.97 1.60 3.20 3.53 2.78 0.79 0 1.1E−02 1.66 3.2299-3p 10378_st1 hsa-asg- hsa-asg- 9.96 10.2 9.47 9.90 0.41 100 8.288.79 6.24 8.51 9.52 8.93 7.30 7.86 7.08 8.06 1.03 89 1.4E−02 1.85 3.6914 914_st1 hsa-asg- hsa-asg- 7.71 8.04 7.75 7.83 0.18 100 6.46 7.185.14 5.71 5.90 7.08 5.29 5.79 5.39 5.99 0.75 44 2.2E−03 1.84 3.6 265265_st2 hsa-asg- hsa-asg- 9.02 8.87 8.60 8.83 0.21 100 6.10 8.52 6.246.84 7.18 8.37 5.73 6.75 7.21 6.99 0.96 100 9.5E−03 1.84 3.6 1195611956_st1 hsa-asg- hsa-asg- 8.65 9.05 8.56 8.76 0.26 100 7.38 7.86 6.177.36 7.80 7.35 6.36 6.23 5.80 6.92 0.78 89 3.0E−03 1.83 3.6 1162611626_st1 hsa-asg- hsa-asg- 9.77 9.97 9.79 9.84 0.11 100 8.22 9.25 7.268.00 8.25 9.10 7.24 7.66 7.13 8.01 0.78 100 2.8E−03 1.83 3.6 48284828_st1 hsa-asg- hsa-asg- 8.62 8.20 8.49 8.44 0.22 100 5.11 8.61 5.175.96 6.94 8.47 5.75 6.04 7.50 6.62 1.33 56 4.5E−02 1.82 3.5 43394339_st2 hsa-asg- hsa-asg- 5.90 6.42 5.59 5.97 0.42 100 4.43 4.95 4.543.91 3.85 5.37 3.77 3.44 3.09 4.15 0.73 0 2.4E−03 1.82 3.5 1011410114_st1 hsa-asg- hsa-asg- 10.1 10.2 10.0 10.14 0.07 100 8.66 9.61 7.297.84 8.41 9.61 7.01 8.17 8.25 8.32 0.90 22 7.0E−03 1.82 3.5 86248624_st2 hsa-asg- hsa-asg- 10.1 10.2 9.95 10.12 0.16 100 9.22 9.37 7.418.49 8.97 9.12 7.52 7.82 6.83 8.30 0.93 100 8.5E−03 1.82 3.5 67286728_st2 hsa-asg- hsa-asg- 5.26 3.52 4.22 4.33 0.88 33 1.65 2.87 2.861.72 2.01 1.97 2.78 2.62 4.16 2.52 0.79 0 7.0E−03 1.81 3.5 4978 4978_st2hsa-asg- hsa-asg- 6.15 6.22 5.66 6.01 0.30 33 3.93 4.40 3.85 4.26 4.364.75 2.92 4.03 5.25 4.20 0.64 0 9.8E−04 1.81 3.5 12598 12598_st1 hsa-hsa- 5.78 6.76 5.94 6.16 0.53 33 4.71 5.57 3.14 3.95 4.30 5.17 3.17 4.664.43 4.35 0.82 0 5.4E−03 1.81 3.5 miR219 miR219_st2 hsa-asg- hsa-asg-9.45 9.99 9.61 9.68 0.28 100 7.81 8.95 6.65 7.47 8.04 8.90 7.25 7.897.87 7.87 0.73 100 2.2E−03 1.81 3.5 5139 5139_st1 hsa-asg- hsa-asg- 4.985.48 5.73 5.40 0.38 67 3.33 4.55 3.06 3.87 2.69 4.28 2.64 3.91 3.97 3.590.69 0 1.6E−03 1.81 3.5 4872 4872_st1 hsa-asg- hsa-asg- 10.4 11.9 11.811.41 0.81 100 10.7 9.96 8.45 10.2 10.9 9.82 9.35 9.73 7.18 9.60 1.17100 3.4E−02 1.81 3.5 11237 11237_st1 hsa-asg- hsa-asg- 4.77 6.12 6.715.87 1.00 67 3.85 4.95 3.67 4.26 4.87 3.48 3.59 4.21 3.65 4.06 0.55 02.2E−03 1.81 3.5 3742 3742_st1 hsa- hsa- 9.54 10.1 10.2 9.96 0.37 1008.24 9.25 7.33 8.24 8.85 8.75 7.67 7.88 7.23 8.16 0.70 100 1.8E−03 1.803.5 cand805 cand805_st2 hsa-miR- hsa-miR- 8.93 7.84 9.60 8.79 0.89 1007.66 6.76 6.71 7.93 7.56 5.83 7.52 6.32 6.65 6.99 0.70 89 4.7E−03 1.793.5 183 183_st1 hsa-asg- hsa-asg- 9.37 8.98 9.06 9.13 0.21 100 6.75 8.896.76 7.19 7.30 9.05 5.64 6.89 7.59 7.34 1.07 89 1.8E−02 1.79 3.5 1057710577_st1 hsa-asg- hsa-asg- 7.05 7.81 6.79 7.22 0.53 100 4.90 6.53 4.815.68 5.94 6.42 4.33 5.04 5.18 5.43 0.76 33 3.8E−03 1.79 3.5 91289128_st1 hsa-asg- hsa-asg- 6.48 6.97 6.75 6.73 0.25 67 4.66 5.90 4.264.76 4.55 6.05 4.49 4.80 5.02 4.94 0.62 0 8.0E−04 1.79 3.5 8435 8435_st2hsa-asg- hsa-asg- 6.21 6.39 6.08 6.23 0.15 67 3.33 6.09 4.58 4.12 4.535.57 1.97 4.49 5.25 4.44 1.23 11 3.5E−02 1.79 3.5 9166 9166_st1 hsa-asg-hsa-asg- 8.37 8.84 8.68 8.63 0.24 100 7.04 8.02 5.85 6.75 7.38 7.59 5.946.44 6.56 6.84 0.74 89 2.4E−03 1.79 3.5 4759 4759_st1 hsa-asg- hsa-asg-7.47 8.27 8.00 7.91 0.41 100 6.35 7.03 4.75 6.59 7.21 6.68 5.19 6.255.09 6.13 0.90 67 8.5E−03 1.79 3.5 8271 8271_st2 hsa-asg- hsa-asg- 7.267.84 7.05 7.38 0.41 100 5.51 6.42 5.21 5.53 5.36 6.33 5.40 4.71 5.915.60 0.54 0 4.2E−04 1.78 3.4 9117 9117_st1 hsa-asg- hsa-asg- 11.3 11.411.1 11.31 0.14 100 9.93 10.5 8.91 9.30 9.94 10.4 8.63 9.34 8.67 9.520.72 100 2.1E−03 1.78 3.4 9078 9078_st2 hsa-asg- hsa-asg- 8.86 9.01 8.878.91 0.08 100 7.72 8.05 5.88 7.02 7.79 7.83 6.35 7.29 6.26 7.13 0.80 893.9E−03 1.78 3.4 8291 8291_st1 hsa-asg- hsa-asg- 5.62 6.58 7.00 6.400.71 67 5.03 5.57 4.17 4.49 4.45 5.25 4.94 3.69 4.02 4.62 0.62 0 1.8E−031.78 3.4 9009 9009_st1 hsa-asg- hsa-asg- 9.16 9.32 9.45 9.31 0.14 1007.86 8.14 6.65 7.37 8.07 7.51 7.11 7.45 7.65 7.53 0.47 100 9.3E−05 1.783.4 1576 1576_st1 hsa-asg- hsa-asg- 5.85 6.53 6.07 6.15 0.35 33 5.355.51 3.31 3.77 3.97 5.49 3.93 4.85 3.17 4.37 0.94 0 1.0E−02 1.78 3.413387 13387_st1 hsa-asg- hsa-asg- 8.02 8.53 7.94 8.16 0.32 100 6.79 7.785.39 5.83 6.54 7.17 5.40 6.57 5.98 6.38 0.81 67 4.6E−03 1.78 3.4 1033810338_st1 hsa-asg- hsa-asg- 9.21 9.62 9.23 9.35 0.23 100 7.74 8.92 6.757.42 7.75 8.56 6.82 7.67 6.57 7.58 0.80 78 4.2E−03 1.78 3.4 1244112441_st1 hsa-asg- hsa-asg- 10.7 11.0 10.7 10.82 0.20 100 9.47 10.0 8.189.09 9.85 9.74 8.25 8.89 7.90 9.04 0.79 67 3.8E−03 1.77 3.4 1228912289_st1 hsa-asg- hsa-asg- 7.93 8.10 7.80 7.94 0.15 100 6.40 7.45 5.516.18 5.99 6.83 5.69 5.89 5.61 6.17 0.63 56 9.0E−04 1.77 3.4 31503150_st1 hsa-asg- hsa-asg- 7.33 7.59 7.44 7.45 0.13 100 5.45 6.81 5.335.63 6.40 6.06 4.73 5.32 5.42 5.68 0.63 33 8.9E−04 1.77 3.4 54135413_st2 hsa-asg- hsa-asg- 9.19 9.20 8.50 8.96 0.40 100 7.86 8.02 6.906.99 7.42 7.88 6.22 7.07 6.36 7.19 0.66 89 1.5E−03 1.77 3.4 88728872_st1 hsa-miR- hsa-miR- 7.37 7.62 6.85 7.28 0.39 100 5.73 6.36 5.244.88 5.90 6.19 4.78 5.42 5.14 5.51 0.56 44 5.5E−04 1.77 3.4 498 498_st1hsa- hsa- 6.98 6.81 6.82 6.87 0.10 100 5.18 6.15 5.26 4.55 4.99 5.693.77 5.06 5.29 5.10 0.67 0 1.3E−03 1.77 3.4 cand630 cand630_st2 hsa-asg-hsa-asg- 9.98 10.3 9.93 10.09 0.24 100 8.74 9.49 7.41 8.22 8.79 9.267.59 7.94 7.44 8.32 0.78 100 3.8E−03 1.77 3.4 6371 6371_st2 hsa-asg-hsa-asg- 8.47 8.62 8.48 8.52 0.09 100 7.13 7.55 5.47 6.78 7.64 7.45 6.096.65 6.08 6.76 0.76 78 2.9E−03 1.76 3.4 13829 13829_st2 hsa-asg-hsa-asg- 5.60 6.39 5.89 5.96 0.40 100 4.09 5.19 2.86 3.40 4.58 4.89 3.774.28 4.73 4.20 0.75 11 3.4E−03 1.76 3.4 12564 12564_st1 hsa-asg-hsa-asg- 6.55 7.28 6.85 6.89 0.37 100 5.14 5.92 3.46 4.91 5.75 4.95 4.695.80 5.58 5.13 0.77 0 3.8E−03 1.76 3.4 3716 3716_st1 hsa-asg- hsa-asg-6.35 5.81 5.76 5.97 0.33 33 3.56 5.35 3.31 2.93 4.24 5.46 3.93 4.31 4.874.22 0.88 0 8.3E−03 1.76 3.4 12799 12799_st1 hsa-asg- hsa-asg- 7.16 7.487.28 7.31 0.16 100 5.03 6.47 4.61 5.28 5.73 6.62 4.94 5.67 5.63 5.550.67 22 1.4E−03 1.76 3.4 8641 8641_st1 hsa- hsa- 5.46 5.37 5.55 5.460.09 67 4.09 4.81 3.91 2.93 4.91 2.35 2.64 3.83 3.87 3.71 0.90 0 8.5E−031.75 3.4 cand360 cand360_st1 hsa-miR- hsa-asg- 7.98 7.89 7.79 7.88 0.09100 7.75 7.03 6.53 7.45 6.62 5.05 5.71 3.83 5.23 6.13 1.28 67 4.4E−021.75 3.4 140 14239_st1 hsa-miR- hsa-miR- 7.47 7.34 8.05 7.62 0.38 1007.73 6.16 6.37 7.22 6.63 5.50 4.78 4.78 5.27 6.05 1.05 44 3.2E−02 1.573.0 140 140_st2 hsa-asg- hsa-asg- 9.43 9.72 9.27 9.47 0.23 100 8.19 8.666.70 7.94 8.49 8.41 6.99 7.05 7.13 7.73 0.76 100 3.3E−03 1.75 3.4 1361313613_st2 hsa-asg- hsa-asg- 8.21 8.35 8.40 8.32 0.10 0 7.33 7.42 5.196.58 7.56 6.95 6.04 5.72 6.37 6.57 0.82 22 5.1E−03 1.74 3.4 175 175_st2hsa-asg- hsa-asg- 9.55 9.67 9.28 9.50 0.20 100 8.20 8.88 7.05 7.56 8.078.62 6.89 7.34 7.23 7.76 0.71 89 2.3E−03 1.74 3.3 6766 6766_st1 hsa-asg-hsa-asg- 7.19 6.88 6.65 6.91 0.27 100 4.66 6.79 3.96 4.91 4.87 6.57 4.075.20 5.48 5.17 0.99 22 1.5E−02 1.74 3.3 6202 6202_st2 hsa-asg- hsa-asg-8.19 8.63 8.38 8.40 0.22 100 6.65 7.75 5.21 6.64 7.46 7.73 6.02 6.266.26 6.66 0.85 89 6.8E−03 1.74 3.3 4070 4070_st2 hsa-asg- hsa-asg- 7.497.78 7.40 7.56 0.20 100 5.80 6.54 5.58 5.51 5.76 6.40 5.22 5.68 5.885.82 0.42 33 4.7E−05 1.74 3.3 5385 5385_st2 hsa-asg- hsa-asg- 7.14 7.306.46 6.97 0.44 100 6.16 5.88 4.58 4.33 5.80 6.69 4.20 4.11 5.33 5.230.96 33 1.4E−02 1.74 3.3 278 278_st1 hsa-miR- hsa-miR- 9.37 8.90 9.349.20 0.27 100 8.38 8.32 7.31 8.03 8.04 6.51 6.91 6.53 7.20 7.47 0.74 1003.1E−03 1.73 3.3 19b 19b_st2 hsa-asg- hsa-asg- 7.37 7.49 7.33 7.40 0.09100 5.96 6.55 4.54 5.68 6.04 6.31 5.12 5.29 5.49 5.66 0.63 44 9.3E−041.73 3.3 5208 5208_st1 hsa-asg- hsa-asg- 6.93 7.63 6.81 7.12 0.44 1005.48 6.13 5.12 5.30 5.10 6.20 4.82 4.98 5.40 5.39 0.48 0 2.7E−04 1.733.3 7027 7027_st2 hsa-asg- hsa-asg- 4.52 5.71 4.42 4.88 0.72 33 3.333.29 2.86 3.08 3.97 2.61 2.78 3.32 3.09 3.15 0.40 0 2.9E−04 1.73 3.32688 2688_st1 hsa-asg- hsa-asg- 6.97 7.11 6.91 7.00 0.11 100 5.35 6.184.61 4.88 5.73 5.89 4.78 5.26 4.73 5.27 0.56 22 4.5E−04 1.73 3.3 47034703_st2 hsa-asg- hsa-asg- 6.90 7.63 7.50 7.34 0.39 100 4.94 7.07 4.904.99 5.90 7.22 3.59 5.31 6.59 5.61 1.19 33 3.6E−02 1.73 3.3 1225312253_st2 hsa-miR- hsa-miR- 11.2 11.1 11.0 11.14 0.07 100 10.9 10.3 8.6910.0 9.47 9.34 9.58 7.33 9.03 9.42 1.03 100 1.8E−02 1.73 3.3 335 335_st1hsa-asg- hsa-asg- 8.20 8.50 8.14 8.28 0.19 100 6.58 7.69 5.09 6.54 7.187.29 5.94 6.46 6.20 6.55 0.78 78 4.2E−03 1.73 3.3 9238 9238_st1 hsa-asg-hsa-asg- 6.38 6.75 6.46 6.53 0.20 100 5.11 5.46 4.02 4.84 5.66 5.63 4.544.35 3.65 4.80 0.72 0 2.6E−03 1.73 3.3 4207 4207_st1 hsa-asg- hsa-asg-7.48 7.57 7.27 7.44 0.15 100 5.70 6.81 5.65 5.54 5.54 6.26 4.73 5.525.68 5.71 0.56 22 4.8E−04 1.72 3.3 8675 8675_st1 hsa-asg- hsa-asg- 8.979.27 9.10 9.11 0.15 100 7.28 8.53 5.47 7.03 8.25 8.67 6.58 7.10 7.587.39 1.02 89 1.7E−02 1.72 3.3 8221 8221_st2 hsa-asg- hsa-asg- 7.80 8.168.16 8.04 0.21 100 6.65 7.38 5.43 6.54 6.82 6.95 5.54 5.83 5.71 6.320.70 56 2.2E−03 1.72 3.3 3728 3728_st1 hsa-asg- hsa-asg- 9.27 9.84 9.449.52 0.30 100 7.47 9.02 5.98 7.51 9.08 8.86 7.01 7.49 7.76 7.80 1.03 891.9E−02 1.72 3.3 11572 11572_st2 hsa- hsa- 7.68 8.21 7.64 7.84 0.32 1006.06 6.94 5.91 5.50 6.00 6.46 5.75 6.19 6.30 6.12 0.42 33 7.3E−05 1.723.3 miR203 miR203_st1 hsa-asg- hsa-asg- 9.76 10.0 9.88 9.88 0.12 1008.58 9.00 7.30 8.04 8.85 9.00 7.45 8.12 7.15 8.16 0.74 100 2.9E−03 1.723.3 9248 9248_st2 hsa-asg- hsa-asg- 6.05 6.26 5.57 5.96 0.36 100 4.904.49 4.12 4.12 4.39 4.10 3.85 3.99 4.24 4.24 0.31 0 1.1E−05 1.72 3.311320 11320_st2 hsa-asg- hsa-asg- 9.04 9.32 9.12 9.16 0.14 100 7.52 8.186.66 7.34 8.01 7.83 6.60 7.58 7.32 7.45 0.55 100 3.8E−04 1.71 3.3 63396339_st1 hsa-asg- hsa-asg- 5.79 6.95 6.88 6.54 0.65 67 5.60 5.83 4.584.66 5.39 4.91 3.68 4.85 3.92 4.82 0.72 0 4.5E−03 1.71 3.3 2315 2315_st2hsa-asg- hsa-asg- 5.27 7.24 7.11 6.54 1.10 67 4.99 5.01 4.84 3.46 5.125.05 4.86 5.52 4.63 4.83 0.57 0 4.6E−03 1.71 3.3 3754 3754_st2 hsa-asg-hsa-asg- 6.64 6.11 6.15 6.30 0.29 33 4.94 6.19 1.79 4.55 4.21 5.66 3.934.98 5.16 4.60 1.26 0 4.8E−02 1.70 3.2 11457 11457_st2 hsa-asg- hsa-asg-5.99 6.25 6.32 6.19 0.17 33 4.55 5.73 3.60 4.19 5.15 5.13 3.40 4.55 4.114.49 0.76 11 3.9E−03 1.70 3.2 2193 2193_st1 hsa-asg- hsa-asg- 6.00 4.474.86 5.11 0.79 100 2.78 3.53 3.39 3.28 3.63 3.37 3.85 2.62 4.32 3.420.52 0 1.4E−03 1.69 3.2 11361 11361_st2 hsa-miR- hsa-miR- 7.55 7.90 7.367.60 0.27 100 6.73 6.17 5.12 5.83 6.14 6.15 5.56 6.04 5.44 5.91 0.48 562.0E−04 1.69 3.2 566 566_st1 hsa-asg- hsa-asg- 7.23 7.57 7.01 7.27 0.28100 5.60 6.48 4.96 5.54 6.06 6.32 4.33 5.49 5.40 5.58 0.67 22 2.0E−031.69 3.2 13598 13598_st1 hsa-asg- hsa-asg- 9.06 9.47 9.17 9.23 0.21 1008.02 8.36 6.10 7.65 8.39 8.46 6.99 7.26 6.64 7.54 0.85 89 7.7E−03 1.693.2 1240 1240_st1 hsa-asg- hsa-asg- 5.50 6.36 6.14 6.00 0.45 33 3.935.21 4.43 4.04 5.25 5.40 1.60 4.11 4.87 4.32 1.16 0 3.7E−02 1.69 3.213080 13080_st1 hsa-asg- hsa-asg- 8.79 10.1 10.2 9.74 0.83 100 9.00 8.687.46 8.25 8.89 8.41 7.19 7.78 6.83 8.06 0.77 100 9.2E−03 1.68 3.2 1152311523_st1 hsa-asg- hsa-asg- 4.98 5.35 4.59 4.98 0.38 33 4.17 4.30 3.393.01 2.45 4.93 2.15 2.93 2.33 3.29 0.98 0 1.7E−02 1.68 3.2 115 115_st1hsa-asg- hsa-asg- 8.58 8.78 8.40 8.59 0.19 100 6.86 7.57 6.93 7.11 7.117.65 6.71 5.80 6.45 6.91 0.56 89 6.0E−04 1.68 3.2 6129 6129_st2 hsa-asg-hsa-asg- 7.82 8.36 7.87 8.02 0.30 100 6.57 7.34 5.69 6.17 6.74 7.05 5.646.00 5.85 6.34 0.61 67 1.2E−03 1.68 3.2 8565 8565_st1 hsa-asg- hsa-asg-10.1 10.3 10.1 10.23 0.13 100 9.06 9.46 7.05 8.60 9.34 9.24 7.92 8.477.84 8.55 0.82 100 6.4E−03 1.68 3.2 7662 7662_st1 hsa-asg- hsa-asg- 5.174.02 4.83 4.67 0.59 67 3.21 2.71 4.47 2.13 2.53 2.17 1.79 3.44 4.54 3.001.00 0 2.2E−02 1.67 3.2 280 280_st1 hsa-asg- hsa-asg- 8.43 8.35 8.078.28 0.19 100 6.62 7.91 6.02 5.97 6.72 7.54 5.35 6.71 6.65 6.61 0.79 785.3E−03 1.67 3.2 13870 13870_st2 hsa-asg- hsa-asg- 10.6 10.8 10.6 10.720.10 100 9.79 9.90 7.99 9.19 9.84 9.79 8.39 9.16 7.37 9.05 0.93 891.2E−02 1.67 3.2 7453 7453_st1 hsa-asg- hsa-asg- 7.18 7.55 7.28 7.340.19 100 6.66 6.55 4.58 5.63 6.20 6.20 4.86 5.49 4.87 5.67 0.78 445.1E−03 1.67 3.2 12246 12246_st2 hsa-asg- hsa-asg- 6.87 7.59 7.55 7.340.40 100 6.27 6.57 4.43 5.88 6.17 6.22 5.51 5.55 4.43 5.67 0.78 566.0E−03 1.67 3.2 8329 8329_st1 hsa-asg- hsa-asg- 5.75 6.50 5.96 6.070.39 100 5.11 4.91 3.54 4.19 5.07 4.75 4.14 4.41 3.53 4.40 0.61 01.3E−03 1.67 3.2 1227 1227_st1 hsa-asg- hsa-asg- 7.38 7.72 7.63 7.580.18 100 6.16 6.79 4.90 5.93 6.36 7.17 5.09 5.46 5.33 5.91 0.78 565.2E−03 1.67 3.2 11977 11977_st1 hsa-asg- hsa-asg- 6.02 6.21 6.41 6.210.20 100 3.66 5.40 3.39 4.69 4.63 5.64 3.93 4.14 5.46 4.55 0.83 07.2E−03 1.67 3.2 14185 14185_st1 hsa-asg- hsa-asg- 6.77 7.38 7.23 7.130.32 100 4.31 6.45 4.96 5.40 5.10 6.51 4.64 6.02 5.80 5.47 0.78 225.7E−03 1.66 3.2 2616 2616_st1 hsa-asg- hsa-asg- 6.97 6.59 6.31 6.620.33 100 4.66 6.87 3.91 4.08 4.70 6.22 3.29 5.11 5.84 4.96 1.17 223.9E−02 1.66 3.2 7461 7461_st2 hsa-asg- hsa-asg- 7.31 7.71 6.95 7.320.38 100 6.23 6.27 5.14 6.23 6.14 6.15 4.64 5.64 4.54 5.67 0.71 563.7E−03 1.66 3.2 809 809_st2 hsa-asg- hsa-asg- 4.52 5.77 5.67 5.32 0.7067 4.24 3.64 1.92 4.08 4.58 4.89 3.05 3.32 3.25 3.66 0.90 0 1.6E−02 1.663.1 1270 1270_st2 hsa-asg- hsa-asg- 7.28 7.34 6.78 7.13 0.31 100 4.556.26 4.58 4.93 5.29 6.04 5.56 5.55 6.57 5.48 0.72 33 3.7E−03 1.65 3.16383 6383_st1 hsa-miR- hsa-miR- 6.22 7.18 6.37 6.59 0.51 100 5.51 4.914.61 4.81 5.70 5.09 3.77 5.22 4.84 4.94 0.56 22 1.1E−03 1.65 3.1 373*373-_st2 hsa-asg- hsa-asg- 8.11 9.28 7.99 8.46 0.71 100 7.15 7.06 5.287.31 8.20 7.17 6.33 6.55 6.27 6.81 0.82 89 1.1E−02 1.65 3.1 70637063_st1 hsa-asg- hsa-asg- 9.84 10.0 9.85 9.92 0.13 100 8.93 9.20 7.348.04 8.38 9.11 7.51 8.24 7.72 8.27 0.69 11 2.6E−03 1.65 3.1 1171411714_st2 hsa-asg- hsa-asg- 9.60 10.2 10.3 10.07 0.41 100 9.22 9.15 7.538.49 9.15 9.26 7.83 8.17 6.97 8.42 0.85 22 9.9E−03 1.65 3.1 46544654_st1 hsa-asg- hsa-asg- 7.50 8.33 7.60 7.81 0.45 100 6.22 7.15 4.966.21 6.66 7.41 5.43 6.00 5.48 6.17 0.81 56 8.4E−03 1.64 3.1 1198211982_st1 hsa-miR- hsa-miR- 8.03 6.27 7.57 7.29 0.91 100 5.14 5.04 6.676.09 6.11 5.17 5.92 5.45 5.27 5.65 0.57 44 3.5E−03 1.64 3.1 409-5p409-5p_st1 hsa-asg- hsa-asg- 8.22 9.15 9.25 8.87 0.57 100 7.95 7.98 5.887.27 8.06 7.86 6.79 7.20 6.10 7.23 0.83 78 1.0E−02 1.64 3.1 86388638_st1 hsa- hsa- 10.2 10.3 10.2 10.29 0.08 100 9.51 9.63 7.62 8.939.28 9.44 7.71 8.44 7.30 8.65 0.91 44 1.3E−02 1.64 3.1 cand711cand711_st1 hsa-asg- hsa-asg- 9.59 9.92 9.63 9.71 0.18 100 8.45 9.076.96 8.06 8.65 8.64 7.39 7.93 7.55 8.08 0.69 100 2.7E−03 1.63 3.1 826826_st1 hsa-asg- hsa-asg- 4.88 4.31 4.93 4.71 0.35 67 3.07 1.96 3.543.52 2.83 1.97 3.40 2.78 4.60 3.07 0.83 0 8.7E−03 1.63 3.1 548 548_st1hsa-asg- hsa-asg- 7.39 7.74 7.22 7.45 0.27 100 4.71 6.92 4.43 6.11 6.196.94 5.43 5.43 6.19 5.82 0.89 33 1.2E−02 1.63 3.1 9126 9126_st2 hsa-asg-hsa-asg- 5.62 5.83 5.67 5.71 0.11 100 3.45 5.64 3.06 4.00 3.28 4.49 3.684.41 4.73 4.08 0.82 11 7.7E−03 1.63 3.1 201 201_st2 hsa-asg- hsa-asg-9.45 9.99 9.36 9.60 0.34 100 7.66 8.87 6.73 7.75 8.75 8.82 7.35 7.818.03 7.97 0.73 100 4.4E−03 1.63 3.1 9867 9867_st2 hsa-asg- hsa-asg- 9.289.28 8.98 9.18 0.17 100 7.11 8.71 6.59 7.22 7.65 8.74 6.71 7.51 7.737.55 0.77 100 5.4E−03 1.63 3.1 11818 11818_st2 hsa-asg- hsa-asg- 7.988.39 8.09 8.15 0.22 100 6.16 8.26 6.15 5.96 6.31 8.06 6.07 6.00 5.776.53 0.94 56 1.6E−02 1.63 3.1 8548 8548_st1 hsa-asg- hsa-asg- 5.69 4.815.23 5.24 0.44 67 3.07 4.99 2.66 3.34 3.33 5.25 3.17 2.78 3.97 3.62 0.930 1.7E−02 1.62 3.1 3236 3236_st1 hsa-asg- hsa-asg- 7.39 7.97 7.06 7.470.46 100 6.32 6.69 4.90 5.17 6.46 6.73 5.19 5.76 5.44 5.85 0.71 444.5E−03 1.62 3.1 1911 1911_st1 hsa- hsa- 7.71 7.75 7.68 7.72 0.04 1006.75 7.27 5.91 5.65 6.24 6.29 5.05 6.14 5.58 6.10 0.66 56 2.0E−03 1.623.1 cand173 cand173_st1 hsa-asg- hsa-asg- 10.0 10.4 10.0 10.17 0.22 1008.95 9.67 7.70 8.60 9.03 9.53 7.87 8.01 7.68 8.56 0.78 100 6.2E−03 1.613.1 6367 6367_st1 hsa-asg- hsa-asg- 8.49 9.04 8.60 8.71 0.29 100 7.577.91 5.94 7.28 7.81 7.85 6.09 6.63 6.83 7.10 0.76 89 5.8E−03 1.61 3.111252 11252_st2 hsa-asg- hsa-asg- 9.94 10.0 9.68 9.87 0.17 100 8.06 9.327.83 7.72 8.40 9.35 7.31 8.76 7.65 8.27 0.74 22 4.7E−03 1.61 3.0 1119311193_st1 hsa-asg- hsa-asg- 5.92 5.86 4.93 5.57 0.55 67 3.45 5.37 3.063.95 4.00 4.75 2.32 4.21 4.54 3.96 0.92 0 1.8E−02 1.61 3.0 1176011760_st1 hsa-asg- hsa-asg- 9.93 10.5 10.2 10.22 0.29 100 8.18 9.56 7.378.18 9.40 9.45 7.83 8.06 9.52 8.62 0.85 100 1.1E−02 1.61 3.0 68706870_st1 hsa-asg- hsa-asg- 5.68 5.66 5.54 5.63 0.08 0 2.61 5.83 3.852.77 2.97 5.20 3.93 4.25 4.76 4.02 1.11 11 3.6E−02 1.61 3.0 14161416_st1 hsa-asg- hsa-asg- 6.96 7.89 7.76 7.54 0.50 100 5.92 6.72 5.435.43 6.44 6.53 5.49 6.15 5.29 5.93 0.55 44 1.1E−03 1.61 3.0 72937293_st1 hsa-asg- hsa-asg- 9.18 9.76 9.52 9.48 0.29 67 8.31 8.61 7.137.79 8.22 8.77 6.91 7.75 7.47 7.88 0.64 0 2.2E−03 1.60 3.0 2290 2290_st2hsa-asg- hsa-asg- 7.44 7.94 7.80 7.73 0.26 100 6.35 7.13 4.84 6.33 6.456.97 5.02 5.94 6.10 6.13 0.78 56 6.7E−03 1.60 3.0 8956 8956_st1 hsa-asg-hsa-asg- 4.42 5.30 5.06 4.93 0.46 67 4.37 3.29 2.86 4.81 3.16 2.85 2.323.07 3.25 3.33 0.78 0 8.0E−03 1.60 3.0 1619 1619_st2 hsa-asg- hsa-asg-7.72 7.79 7.44 7.65 0.19 100 6.39 6.71 5.26 6.03 6.76 6.35 5.35 6.085.64 6.06 0.55 56 7.4E−04 1.59 3.0 14025 14025_st1 hsa-asg- hsa-asg-9.85 10.0 9.73 9.86 0.14 100 8.34 9.16 7.56 7.88 8.40 9.24 7.68 8.787.46 8.28 0.68 11 2.9E−03 1.59 3.0 9150 9150_st1 hsa-asg- hsa-asg- 8.158.23 7.91 8.09 0.17 100 6.75 7.58 5.72 5.95 6.52 7.25 5.92 6.81 6.066.51 0.64 89 2.1E−03 1.59 3.0 11089 11089_st1 hsa-asg- hsa-asg- 6.256.54 6.49 6.43 0.16 33 4.37 6.05 3.85 4.15 5.07 5.76 4.69 4.76 4.87 4.840.71 0 4.0E−03 1.58 3.0 7043 7043_st2 hsa-asg- hsa-asg- 8.54 8.77 8.378.56 0.20 100 7.04 7.93 5.85 6.64 7.74 7.80 6.81 6.26 6.73 6.98 0.72 894.4E−03 1.58 3.0 9412 9412_st2 hsa-asg- hsa-asg- 7.81 7.78 7.48 7.690.18 100 5.32 7.35 5.30 5.25 6.40 7.47 5.46 5.27 7.12 6.11 0.98 442.2E−02 1.58 3.0 10199 10199_st1 hsa-miR- hsa-miR- 10.4 10.0 10.6 10.380.27 100 9.62 9.27 8.82 9.68 9.16 8.64 7.76 7.45 8.75 8.80 0.77 1006.7E−03 1.58 3.0 218 218_st2 hsa- hsa- 8.79 9.07 8.87 8.91 0.15 67 7.738.07 5.92 7.30 7.94 7.95 6.88 7.46 6.72 7.33 0.71 56 4.0E−03 1.58 3.0cand441 cand441_st2 hsa-asg- hsa-asg- 6.83 7.28 7.30 7.14 0.27 100 5.146.43 4.26 5.25 6.01 6.74 4.54 5.58 6.06 5.56 0.84 33 1.0E−02 1.58 3.01239 1239_st1 hsa-asg- hsa-asg- 8.39 8.95 8.78 8.70 0.29 100 7.41 8.455.88 7.35 7.66 8.16 6.12 6.92 6.21 7.13 0.91 89 1.7E−02 1.57 3.0 64376437_st1 hsa- hsa- 6.78 7.49 7.16 7.14 0.36 100 5.73 6.67 4.17 5.25 6.206.52 4.73 5.63 5.25 5.57 0.82 33 1.0E−02 1.57 3.0 cand484 cand484_st2hsa-asg- hsa-asg- 8.58 8.94 8.48 8.66 0.25 100 7.24 8.15 5.85 6.82 7.428.35 6.47 7.54 5.98 7.09 0.88 89 1.4E−02 1.57 3.0 9713 9713_st1 hsa-hsa- 6.73 7.08 6.53 6.78 0.28 100 4.85 5.82 4.54 5.35 4.70 5.93 4.385.56 5.75 5.21 0.60 11 1.5E−03 1.57 3.0 cand676 cand676_st2 hsa-asg-hsa-asg- 8.27 8.37 7.97 8.20 0.21 100 6.65 7.78 6.17 6.40 6.67 7.58 5.696.91 5.88 6.64 0.71 100 4.3E−03 1.57 3.0 9130 9130_st2 hsa-asg- hsa-asg-10.6 10.9 10.5 10.71 0.18 100 8.88 10.0 8.27 8.95 9.79 9.84 8.28 8.929.35 9.14 0.65 78 2.4E−03 1.57 3.0 377 377_st2 hsa-asg- hsa-asg- 6.427.09 6.80 6.77 0.34 100 6.32 5.04 3.91 5.10 6.23 4.65 5.05 6.32 4.245.21 0.90 33 1.7E−02 1.56 3.0 8280 8280_st2 hsa-asg- hsa-asg- 7.14 7.117.03 7.09 0.05 100 5.32 6.42 5.06 5.06 5.46 6.52 4.14 5.80 6.01 5.530.75 33 5.8E−03 1.56 2.9 114 114_st2 hsa- hsa- 5.56 6.20 5.84 5.87 0.32100 4.76 4.71 4.30 4.33 4.39 4.55 3.77 3.44 4.54 4.31 0.44 0 2.2E−041.56 2.9 cand239 cand239_st2 hsa-asg- hsa-asg- 5.17 5.75 5.43 5.45 0.29100 3.45 4.99 3.06 3.73 4.42 4.70 3.40 3.44 3.87 3.89 0.66 11 3.2E−031.56 2.9 3829 3829_st1 hsa-miR- hsa-miR- 6.00 3.48 2.64 4.04 1.75 333.33 2.87 2.43 2.60 3.22 1.29 2.64 2.62 1.38 2.49 0.72 0 4.3E−02 1.552.9 383 383_st1 hsa-asg- hsa-asg- 10.4 10.8 10.0 10.41 0.40 100 9.079.37 7.35 9.28 10.0 9.45 8.23 8.58 8.34 8.86 0.81 100 1.0E−02 1.55 2.93591 3591_st1 hsa-asg- hsa-asg- 5.82 6.58 6.48 6.29 0.41 67 5.14 4.523.85 4.79 4.89 4.43 3.68 5.60 5.74 4.74 0.71 0 5.3E−03 1.55 2.9 94929492_st1 hsa-asg- hsa-asg- 7.09 7.81 7.84 7.58 0.42 100 6.16 6.85 5.265.78 6.57 6.69 5.59 5.58 5.77 6.03 0.57 44 1.5E−03 1.55 2.9 11681168_st1 hsa-asg- hsa-asg- 7.14 7.25 6.75 7.05 0.27 100 4.71 6.60 3.855.27 6.00 7.27 5.29 5.27 5.25 5.50 1.01 22 2.8E−02 1.55 2.9 84508450_st1 hsa-asg- hsa-asg- 7.58 8.24 7.94 7.92 0.33 100 6.49 7.44 4.586.28 6.87 7.36 6.30 6.17 5.85 6.37 0.86 78 1.4E−02 1.55 2.9 72187218_st1 hsa-miR- hsa-miR- 5.00 3.20 4.11 4.10 0.90 33 2.61 1.75 2.662.93 3.44 1.97 3.29 3.00 1.38 2.56 0.71 0 1.1E−02 1.54 2.9 369-3p369-3p_st1 hsa-asg- hsa-asg- 6.71 7.41 6.75 6.96 0.40 100 5.89 6.45 4.175.37 5.90 6.27 4.38 5.68 4.63 5.42 0.83 0 1.2E−02 1.54 2.9 5272 5272_st2hsa-asg- hsa-asg- 6.49 7.05 6.74 6.76 0.28 67 5.85 6.15 5.30 5.12 4.704.73 4.73 4.80 5.58 5.22 0.54 0 9.3E−04 1.54 2.9 6961 6961_st2 hsa-asg-hsa-asg- 7.94 7.43 7.46 7.61 0.29 0 5.60 6.42 6.43 6.33 5.78 6.22 6.765.42 5.63 6.06 0.47 11 3.5E−04 1.54 2.9 8476 8476_st1 hsa-asg- hsa-asg-6.43 7.39 6.58 6.80 0.52 67 5.29 6.23 3.85 5.16 5.96 6.09 4.44 5.20 5.125.26 0.77 0 1.0E−02 1.54 2.9 9124 9124_st1 hsa-asg- hsa-asg- 7.09 7.767.08 7.31 0.39 100 6.80 6.91 4.43 5.78 6.24 6.73 4.98 5.68 4.36 5.771.00 44 2.9E−02 1.54 2.9 9577 9577_st1 hsa-asg- hsa-asg- 7.84 8.39 8.218.15 0.28 100 6.42 7.58 6.33 6.06 6.93 7.47 5.32 7.06 6.28 6.60 0.72 895.6E−03 1.54 2.9 5399 5399_st1 hsa-asg- hsa-asg- 7.38 8.04 7.51 7.640.35 100 5.89 7.10 5.30 6.16 6.68 7.29 5.40 5.98 5.12 6.10 0.78 449.2E−03 1.54 2.9 10815 10815_st1 hsa-asg- hsa-asg- 7.78 8.42 8.12 8.110.32 100 7.22 7.23 5.79 6.81 6.62 7.01 5.90 6.21 6.36 6.57 0.54 891.0E−03 1.54 2.9 14066 14066_st2 hsa-asg- hsa-asg- 8.55 9.35 9.47 9.120.50 100 8.15 8.15 5.74 7.66 8.37 7.69 7.27 8.33 6.92 7.59 0.85 891.5E−02 1.54 2.9 109 109_st1 hsa-asg- hsa-asg- 9.51 9.67 9.49 9.55 0.10100 8.95 8.90 7.30 7.84 8.41 8.62 6.90 8.10 7.18 8.02 0.76 100 7.2E−031.53 2.9 9625 9625_st2 hsa-asg- hsa-asg- 7.33 7.35 7.42 7.37 0.05 1005.85 6.91 5.98 5.32 5.63 6.32 4.49 6.47 5.53 5.83 0.71 44 4.6E−03 1.532.9 8003 8003_st2 hsa-miR-31 hsa-miR- 11.7 9.58 11.0 10.77 1.08 100 10.49.79 8.95 10.1 8.71 8.60 7.73 8.68 10.1 9.23 0.91 100 3.5E−02 1.53 2.931_st2 hsa-asg- hsa-asg- 5.17 5.95 5.41 5.51 0.40 67 3.85 4.86 3.79 3.573.59 4.93 3.17 4.07 3.97 3.98 0.58 0 1.9E−03 1.53 2.9 12271 12271_st1hsa-asg- hsa-asg- 7.94 8.45 8.15 8.18 0.26 0 6.06 7.50 5.53 6.41 7.507.38 6.23 6.85 6.37 6.65 0.70 22 4.8E−03 1.53 2.9 9165 9165_st1 hsa-asg-hsa-asg- 8.22 8.45 8.38 8.35 0.11 100 6.88 7.76 5.94 6.48 7.13 7.43 5.846.82 7.12 6.82 0.64 78 2.6E−03 1.53 2.9 4847 4847_st1 hsa-asg- hsa-asg-8.33 7.80 7.43 7.85 0.45 100 6.96 7.57 5.82 5.51 6.76 7.05 5.80 6.445.02 6.33 0.83 67 1.4E−02 1.53 2.9 8991 8991_st2 hsa-miR- hsa-miR- 10.110.0 10.0 10.08 0.06 100 9.33 9.03 8.26 9.31 8.40 9.13 7.93 7.39 8.198.55 0.68 100 3.7E−03 1.53 2.9 30a-3p 30a-3p_st1 hsa-asg- hsa-asg- 7.608.21 7.87 7.89 0.31 100 6.84 7.40 5.49 6.59 6.90 7.28 5.37 5.79 5.666.37 0.79 56 9.9E−03 1.53 2.9 12953 12953_st2 hsa-asg- hsa-asg- 6.917.46 6.94 7.10 0.31 100 5.29 6.33 5.39 5.54 6.03 6.45 4.86 5.60 4.705.58 0.61 22 2.2E−03 1.52 2.9 7336 7336_st1 hsa-asg- hsa-asg- 9.75 10.29.56 9.86 0.37 100 8.43 9.00 6.93 8.12 9.37 8.94 7.81 8.27 8.15 8.340.73 100 6.6E−03 1.52 2.9 3539 3539_st2 hsa-asg- hsa-asg- 6.07 5.75 5.985.93 0.16 100 5.03 5.49 3.06 3.46 5.42 5.27 2.78 4.87 4.32 4.41 1.06 03.6E−02 1.52 2.9 4261 4261_st1 hsa-asg- hsa-asg- 6.33 7.36 6.68 6.790.52 100 4.55 6.56 4.68 5.02 5.07 5.98 4.90 5.19 5.49 5.27 0.64 114.3E−03 1.52 2.9 1337 1337_st1 hsa- hsa- 5.84 7.44 7.50 6.93 0.94 675.51 6.13 4.96 4.71 5.17 6.07 5.69 5.31 5.12 5.41 0.49 0 3.6E−03 1.522.9 miR223 miR223_st2 hsa-asg- hsa-asg- 6.04 6.44 6.54 6.34 0.27 33 2.786.32 3.60 4.71 4.70 5.74 4.49 5.24 5.82 4.82 1.12 0 4.7E−02 1.52 2.94850 4850_st2 hsa-asg- hsa-asg- 9.87 10.1 10.1 10.07 0.17 100 9.63 9.437.58 8.40 9.30 8.97 7.96 8.90 6.78 8.55 0.95 100 2.3E−02 1.52 2.9 17261726_st1 hsa-asg- hsa-asg- 6.85 7.60 7.03 7.16 0.39 100 6.12 6.35 5.555.06 5.97 6.37 5.16 5.09 5.12 5.64 0.56 33 1.6E−03 1.52 2.9 57355735_st2 hsa- hsa- 4.92 6.05 6.24 5.74 0.72 100 4.76 5.04 3.54 4.71 5.154.18 2.64 3.99 3.97 4.22 0.80 11 1.6E−02 1.52 2.9 cand330 cand330_st2hsa-asg- hsa-asg- 7.03 7.50 7.03 7.19 0.27 100 5.62 6.45 4.84 5.67 6.646.23 4.90 5.46 5.25 5.67 0.65 11 3.2E−03 1.52 2.9 1187 1187_st2 hsa-asg-hsa-asg- 9.33 9.85 9.61 9.60 0.26 100 8.74 9.13 7.31 8.06 8.63 8.92 7.197.82 6.91 8.08 0.82 100 1.1E−02 1.52 2.9 12581 12581_st1 hsa-asg-hsa-asg- 5.58 5.51 4.75 5.28 0.46 67 3.85 4.55 4.02 3.15 3.39 4.03 4.072.62 4.24 3.77 0.60 0 2.8E−03 1.52 2.9 12760 12760_st2 hsa-asg- hsa-asg-10.7 11.1 10.8 10.93 0.23 100 9.76 10.2 7.85 9.77 10.6 10.1 9.03 9.318.10 9.42 0.95 78 2.3E−02 1.51 2.9 1192 1192_st1 hsa-asg- hsa-asg- 10.010.6 10.2 10.29 0.30 100 9.15 9.42 8.00 9.05 9.49 9.21 8.45 8.37 7.838.77 0.62 56 2.6E−03 1.51 2.9 6063 6063_st2 hsa-asg- hsa-asg- 5.69 6.296.75 6.24 0.53 33 4.99 4.97 4.72 4.81 5.48 5.03 4.73 3.49 4.36 4.73 0.550 2.0E−03 1.51 2.9 8893 8893_st1 hsa-asg- hsa-asg- 7.01 7.46 6.86 7.110.31 100 5.78 6.11 5.39 5.87 5.63 6.05 4.90 5.68 4.97 5.60 0.43 02.6E−04 1.51 2.8 1922 1922_st2 hsa-asg- hsa-asg- 7.08 7.01 6.63 6.900.24 100 5.48 5.99 4.50 5.32 5.74 6.11 4.94 5.20 5.25 5.39 0.51 226.6E−04 1.51 2.8 6618 6618_st2 hsa-asg- hsa-asg- 9.24 9.94 9.49 9.560.36 100 7.90 9.00 7.67 7.40 8.36 9.04 7.28 8.07 7.72 8.05 0.64 1003.3E−03 1.51 2.8 9261 9261_st1 hsa-asg- hsa-asg- 5.90 5.89 5.84 5.880.03 100 3.66 5.12 3.91 4.49 4.39 5.34 3.17 3.74 5.53 4.37 0.82 01.1E−02 1.51 2.8 2702 2702_st1 hsa- hsa- 7.15 7.45 7.19 7.26 0.16 1005.70 7.04 5.12 5.14 5.80 6.71 3.77 5.92 6.65 5.76 1.01 33 3.2E−02 1.502.8 cand523 cand523_st1 hsa-asg- hsa-asg- 10.2 10.3 10.0 10.21 0.13 1008.73 9.85 8.29 8.24 9.08 9.83 7.44 8.24 8.70 8.71 0.78 100 9.4E−03 1.502.8 8576 8576_st2 hsa-asg- hsa-asg- 9.56 9.88 9.46 9.63 0.22 100 8.478.99 6.70 8.24 9.27 9.00 7.37 7.92 7.23 8.13 0.90 100 1.9E−02 1.50 2.85085 5085_st2 hsa-asg- hsa-asg- 9.46 9.52 9.08 9.35 0.24 100 7.96 8.817.19 7.44 8.12 8.38 6.77 7.99 7.99 7.85 0.62 100 2.6E−03 1.50 2.8 1215012150_st1 hsa-asg- hsa-asg- 8.52 8.73 8.27 8.51 0.23 100 6.10 7.96 6.686.57 7.18 8.18 5.82 7.05 7.49 7.01 0.80 100 1.0E−02 1.50 2.8 36373637_st1 hsa-asg- hsa-asg- 4.92 5.72 4.90 5.18 0.47 33 3.45 4.61 2.664.26 3.68 4.43 3.40 3.20 3.39 3.67 0.64 0 4.1E−03 1.50 2.8 1281412814_st2 hsa-asg- hsa-asg- 10.1 10.6 10.3 10.37 0.24 100 9.33 9.66 7.848.80 9.87 9.43 8.59 8.78 7.59 8.88 0.79 56 1.0E−02 1.50 2.8 18601860_st1 hsa- hsa- 6.77 7.43 6.65 6.95 0.42 100 5.80 6.33 3.14 5.08 5.826.32 4.86 5.78 5.96 5.45 1.00 0 3.3E−02 1.50 2.8 cand197 cand197_st2hsa-asg- hsa-asg- 9.86 10.0 9.91 9.95 0.11 100 8.57 9.40 7.66 8.51 9.188.83 7.73 8.35 7.85 8.45 0.62 100 2.5E−03 1.50 2.8 8254 8254_st2hsa-asg- hsa-asg- 5.83 6.77 6.74 6.45 0.54 100 4.71 6.53 3.91 4.12 4.876.49 4.27 4.41 5.27 4.95 0.97 22 3.2E−02 1.49 2.8 8069 8069_st1 hsa-asg-hsa-asg- 10.9 11.0 10.7 10.92 0.14 100 10.1 10.3 8.52 9.31 10.1 10.18.67 9.49 8.16 9.43 0.82 100 1.2E−02 1.49 2.8 2906 2906_st2 hsa-asg-hsa-asg- 7.24 6.82 6.80 6.95 0.25 100 5.11 6.50 5.45 4.93 6.02 5.78 4.785.77 4.84 5.46 0.60 22 2.1E−03 1.49 2.8 11795 11795_st2 hsa-asg-hsa-asg- 8.20 8.29 8.07 8.19 0.11 100 6.97 7.32 6.17 6.81 7.15 7.07 5.906.37 6.51 6.70 0.48 100 4.3E−04 1.49 2.8 9511 9511_st2 hsa-asg- hsa-asg-7.42 8.08 8.04 7.85 0.37 100 7.02 7.47 5.12 6.58 6.78 7.09 5.54 6.425.20 6.36 0.87 67 1.8E−02 1.49 2.8 8763 8763_st2 hsa-asg- hsa-asg- 10.510.8 10.5 10.65 0.18 100 9.42 10.0 8.35 9.28 9.83 9.77 8.51 8.97 8.259.16 0.67 78 4.2E−03 1.49 2.8 9507 9507_st1 hsa-asg- hsa-asg- 7.89 8.938.12 8.31 0.55 100 6.80 7.36 5.41 6.90 7.59 7.33 6.38 6.67 6.99 6.820.65 89 5.3E−03 1.49 2.8 8173 8173_st1 hsa-asg- hsa-asg- 9.05 9.27 9.059.12 0.13 100 7.55 8.54 6.66 7.48 8.11 8.46 6.85 7.40 7.63 7.63 0.65 1003.3E−03 1.49 2.8 1893 1893_st2 hsa-asg- hsa-asg- 5.02 5.35 5.06 5.140.18 67 4.02 4.16 3.06 3.01 4.60 2.99 2.92 4.14 3.97 3.65 0.65 0 3.4E−031.49 2.8 12154 12154_st1 hsa-asg- hsa-asg- 4.16 5.86 5.65 5.22 0.93 674.49 4.37 2.66 3.82 4.21 4.55 3.93 3.74 1.86 3.74 0.91 0 3.4E−02 1.492.8 1203 1203_st1 hsa-asg- hsa-asg- 6.36 6.96 6.36 6.56 0.35 100 5.755.99 4.78 5.25 5.49 6.26 3.05 5.17 3.92 5.07 1.03 22 3.7E−02 1.49 2.814148 14148_st2 hsa-asg- hsa-asg- 6.57 8.00 8.02 7.53 0.83 100 6.46 6.645.06 6.14 7.07 6.29 5.43 6.54 4.81 6.05 0.77 67 1.7E−02 1.48 2.8 758758_st1 hsa-asg- hsa-asg- 8.40 9.13 8.47 8.67 0.40 100 6.39 7.91 6.266.96 7.62 7.91 6.29 7.49 7.85 7.19 0.72 100 7.6E−03 1.48 2.8 92279227_st1 hsa-asg- hsa-asg- 10.5 11.0 10.8 10.79 0.22 100 9.54 10.1 8.399.37 10.2 10.0 8.80 9.21 8.12 9.31 0.75 100 8.4E−03 1.48 2.8 92739273_st2 hsa-asg- hsa-asg- 9.81 9.90 9.57 9.76 0.17 100 9.10 9.15 7.268.05 8.80 9.16 7.24 8.31 7.49 8.28 0.81 100 1.2E−02 1.48 2.8 92669266_st1 hsa-asg- hsa-asg- 5.79 5.70 5.77 5.75 0.05 100 4.99 5.33 3.063.46 4.95 5.17 4.00 3.64 3.87 4.27 0.84 0 1.4E−02 1.48 2.8 4315 4315_st2hsa-miR- hsa-miR- 8.87 7.86 8.76 8.50 0.55 33 7.43 7.60 6.23 8.75 7.866.49 6.51 5.62 6.69 7.02 0.96 33 3.3E−02 1.48 2.8 497 497_st1 hsa-miR-hsa- 9.66 8.52 9.65 9.28 0.65 67 8.20 8.56 7.51 9.37 8.88 7.78 7.45 7.137.83 8.08 0.74 89 3.1E−02 1.20 2.3 497 miR43_st1 hsa-asg- hsa-asg- 10.310.7 10.2 10.46 0.27 100 8.33 10.2 7.59 8.59 9.57 10.3 7.88 8.75 9.608.98 0.99 100 3.3E−02 1.48 2.8 542 542_st1 hsa-asg- hsa-asg- 7.68 8.117.63 7.81 0.27 100 6.55 7.46 5.35 6.56 7.00 7.20 5.09 6.21 5.58 6.330.84 67 1.5E−02 1.47 2.8 7169 7169_st1 hsa-asg- hsa-asg- 8.82 9.05 9.078.98 0.14 100 7.46 8.59 6.44 7.10 7.91 8.07 6.80 7.71 7.50 7.51 0.66 1004.0E−03 1.47 2.8 5019 5019_st1 hsa-asg- hsa-asg- 8.07 8.22 7.72 8.000.26 100 7.11 7.19 6.67 5.45 6.65 6.93 5.66 6.72 6.41 6.53 0.61 782.5E−03 1.47 2.8 938 938_st2 hsa-asg- hsa-asg- 7.50 7.83 8.40 7.91 0.450 7.05 6.35 7.01 7.73 6.67 5.80 5.79 5.15 6.39 6.44 0.78 11 1.2E−02 1.472.8 14150 14150_st1 hsa-asg- hsa-asg- 8.25 8.48 8.32 8.35 0.12 100 6.717.85 5.72 7.17 7.23 7.65 6.11 6.71 6.80 6.88 0.68 89 4.9E−03 1.47 2.87224 7224_st2 hsa-asg- hsa-asg- 6.42 6.45 6.79 6.56 0.21 33 4.02 6.034.26 4.91 5.36 5.53 4.54 5.27 5.91 5.09 0.71 0 6.5E−03 1.46 2.8 48624862_st1 hsa-asg- hsa-asg- 10.7 11.1 10.7 10.90 0.25 100 9.42 10.2 8.199.24 10.3 10.1 8.71 9.04 9.72 9.44 0.73 89 7.9E−03 1.46 2.7 78597859_st1 hsa-asg- hsa-asg- 7.67 7.84 7.15 7.55 0.36 100 5.87 6.95 5.795.83 6.10 7.41 4.82 6.82 5.29 6.10 0.83 33 1.6E−02 1.46 2.7 68316831_st2 hsa-asg- hsa-asg- 8.15 8.29 8.09 8.18 0.10 100 6.97 7.58 5.716.54 7.03 7.69 5.90 6.59 6.46 6.72 0.68 89 4.7E−03 1.46 2.7 89578957_st2 hsa-asg- hsa-asg- 6.28 6.31 6.49 6.36 0.11 100 4.49 6.25 4.434.46 4.89 5.85 3.77 4.60 5.40 4.90 0.78 22 1.1E−02 1.46 2.7 1150611506_st1 hsa-asg- hsa-asg- 9.52 9.96 9.78 9.75 0.22 100 8.67 9.06 7.068.30 8.97 9.04 7.83 8.10 7.68 8.30 0.70 11 6.2E−03 1.45 2.7 77647764_st2 hsa-asg- hsa-asg- 7.19 7.97 8.11 7.76 0.50 100 6.57 6.82 6.175.64 6.77 6.07 5.84 6.74 6.19 6.31 0.43 44 6.2E−04 1.45 2.7 32913291_st2 hsa-asg- hsa-asg- 6.95 7.49 7.15 7.20 0.27 100 5.57 7.08 4.435.45 6.33 6.59 4.33 5.68 6.28 5.75 0.94 44 2.8E−02 1.45 2.7 94839483_st1 hsa-asg- hsa-asg- 7.57 7.85 7.85 7.76 0.16 100 6.96 7.18 5.746.27 6.67 6.52 5.54 6.57 5.35 6.31 0.64 67 3.7E−03 1.45 2.7 53265326_st2 hsa-asg- hsa-asg- 7.42 8.09 7.61 7.71 0.35 100 6.52 7.11 5.356.04 6.33 6.91 5.43 6.44 6.24 6.26 0.59 78 2.9E−03 1.44 2.7 48144814_st1 hsa-asg- hsa-asg- 9.95 10.2 9.82 10.00 0.21 100 8.53 9.45 7.488.85 9.25 9.34 7.76 8.38 8.01 8.56 0.72 100 7.5E−03 1.44 2.7 60226022_st2 hsa-asg- hsa-asg- 9.96 10.3 9.95 10.07 0.20 100 8.51 9.49 7.478.51 9.06 9.76 7.67 8.84 8.43 8.64 0.76 100 1.0E−02 1.43 2.7 21992199_st1 hsa-asg- hsa-asg- 8.92 9.15 9.03 9.03 0.12 100 7.33 8.56 6.467.48 8.18 8.50 6.84 7.38 7.65 7.60 0.71 100 7.3E−03 1.43 2.7 72007200_st2 hsa-asg- hsa-asg- 6.60 5.74 6.15 6.16 0.43 100 3.66 6.34 4.544.93 4.36 6.07 3.77 4.73 4.16 4.73 0.93 22 3.1E−02 1.43 2.7 44584458_st1 hsa-asg- hsa-asg- 7.10 7.82 7.58 7.50 0.37 100 6.16 7.05 4.906.44 6.74 6.82 5.16 5.52 5.82 6.07 0.76 56 1.2E−02 1.43 2.7 82558255_st2 hsa-asg- hsa-asg- 5.37 3.91 3.00 4.09 1.19 33 2.05 2.54 3.672.51 1.46 2.85 2.64 2.45 3.77 2.66 0.72 0 2.8E−02 1.43 2.7 1390813908_st2 hsa-asg- hsa-asg- 10.5 10.5 10.2 10.44 0.21 100 8.94 9.79 8.628.62 9.12 9.81 8.34 9.59 8.28 9.01 0.60 67 2.8E−03 1.43 2.7 814 814_st1hsa-asg- hsa-asg- 2.38 4.31 5.20 3.96 1.44 33 2.78 2.36 1.92 3.52 3.392.35 2.32 1.88 2.33 2.54 0.58 0 2.7E−02 1.43 2.7 4037 4037_st1 hsa-asg-hsa-asg- 9.42 9.88 9.75 9.68 0.24 100 8.50 9.51 7.11 8.05 8.61 8.94 7.077.99 8.55 8.26 0.80 100 1.4E−02 1.43 2.7 8866 8866_st1 hsa-asg- hsa-asg-9.03 9.62 9.11 9.25 0.32 100 7.74 8.73 6.83 7.65 8.46 8.47 6.96 7.787.82 7.83 0.65 100 5.3E−03 1.42 2.7 8623 8623_st1 hsa-asg- hsa-asg- 9.7110.0 9.39 9.71 0.31 100 8.55 8.92 7.54 8.09 8.78 8.96 7.63 8.30 7.798.29 0.55 100 1.9E−03 1.42 2.7 12014 12014_st2 hsa-asg- hsa-asg- 5.566.33 6.28 6.06 0.43 100 4.76 4.76 4.39 5.12 5.35 5.40 4.38 4.07 3.534.64 0.61 11 4.4E−03 1.42 2.7 13471 13471_st1 hsa-asg- hsa-asg- 6.116.35 6.28 6.25 0.12 100 4.49 6.12 3.91 3.95 5.45 5.80 4.14 4.73 4.894.83 0.81 11 1.4E−02 1.42 2.7 2149 2149_st1 hsa-asg- hsa-asg- 5.95 6.166.40 6.17 0.22 100 4.31 6.18 5.09 4.19 4.93 4.95 3.40 4.41 5.31 4.750.79 11 1.3E−02 1.42 2.7 11930 11930_st1 hsa-asg- hsa-asg- 8.18 8.498.23 8.30 0.17 100 7.02 7.84 4.84 6.66 7.54 7.05 6.60 6.52 7.82 6.880.92 89 2.7E−02 1.42 2.7 11945 11945_st1 hsa-asg- hsa-asg- 8.14 8.137.98 8.08 0.09 100 5.65 7.77 5.80 6.18 6.92 7.17 5.56 7.08 7.86 6.670.89 78 2.3E−02 1.42 2.7 4002 4002_st1 hsa-asg- hsa-asg- 8.49 8.62 8.348.48 0.14 100 6.37 8.00 6.65 6.52 6.87 7.80 6.59 7.32 7.49 7.07 0.60 1002.8E−03 1.42 2.7 9149 9149_st1 hsa-asg- hsa-asg- 6.53 6.66 6.05 6.410.32 67 5.14 6.07 4.26 4.79 4.89 5.52 3.50 5.13 5.71 5.00 0.78 111.3E−02 1.41 2.7 1727 1727_st2 hsa-asg- hsa-asg- 10.2 10.6 10.4 10.460.23 100 9.89 10.0 7.94 8.95 9.82 9.96 8.43 8.95 7.49 9.05 0.94 783.1E−02 1.41 2.7 6466 6466_st1 hsa-asg- hsa-asg- 7.00 6.80 6.76 6.850.13 100 6.16 5.85 4.72 5.27 6.19 6.09 4.69 5.37 4.63 5.44 0.66 04.9E−03 1.41 2.7 6675 6675_st1 hsa-asg- hsa-asg- 7.78 8.31 7.66 7.910.35 0 7.36 7.13 5.60 7.02 7.21 6.82 5.93 6.18 5.27 6.50 0.77 11 1.3E−021.41 2.7 5288 5288_st1 hsa-asg- hsa-asg- 9.56 10.4 10.3 10.13 0.50 1009.25 9.25 7.42 8.69 9.25 9.08 8.33 8.71 8.53 8.72 0.60 100 4.3E−03 1.412.7 10638 10638_st2 hsa-asg- hsa-asg- 4.03 5.19 4.68 4.63 0.58 33 3.933.53 2.66 3.82 3.93 2.85 3.50 2.27 2.54 3.22 0.65 0 7.7E−03 1.41 2.74283 4283_st2 hsa-asg- hsa-asg- 6.25 6.39 6.46 6.37 0.11 33 5.25 5.684.26 5.34 5.61 5.07 4.44 4.38 4.63 4.96 0.55 0 1.5E−03 1.41 2.7 35873587_st1 hsa-miR- hsa-miR- 8.86 7.03 8.24 8.04 0.93 100 6.89 7.22 7.376.61 6.11 6.50 6.41 6.62 6.01 6.64 0.46 78 4.8E−03 1.41 2.6 503 503_st1hsa-miR- hsa- 8.94 7.31 8.35 8.20 0.83 100 7.11 7.33 7.30 6.52 6.57 6.686.86 6.89 6.76 6.89 0.30 100 1.5E−03 1.31 2.5 503 miR141_st1 hsa-asg-hsa-asg- 6.04 6.44 6.00 6.16 0.24 33 5.35 5.92 4.34 4.84 4.47 5.27 3.934.66 4.02 4.76 0.66 11 5.5E−03 1.41 2.6 8193 8193_st2 hsa-asg- hsa-asg-9.76 10.0 9.66 9.81 0.17 100 8.49 9.10 7.26 8.32 9.07 8.97 7.92 8.547.94 8.40 0.62 100 3.5E−03 1.40 2.6 4020 4020_st2 hsa-asg- hsa-asg- 7.988.20 7.79 7.99 0.20 100 6.92 7.25 5.64 6.36 6.98 6.91 6.02 6.75 6.466.59 0.52 89 1.1E−03 1.40 2.6 7062 7062_st1 hsa-asg- hsa-asg- 6.83 7.026.44 6.76 0.29 100 5.57 6.26 3.96 5.69 5.53 6.20 3.85 5.49 5.68 5.360.87 0 2.3E−02 1.40 2.6 1157 1157_st1 hsa-asg- hsa-asg- 6.87 7.19 6.556.87 0.32 100 4.61 6.72 4.61 5.45 5.77 6.40 5.25 5.19 5.18 5.47 0.73 221.0E−02 1.40 2.6 12379 12379_st1 hsa-asg- hsa-asg- 9.53 10.0 9.52 9.690.28 100 8.08 8.80 7.14 8.05 8.52 9.22 7.87 8.73 8.19 8.29 0.61 1003.7E−03 1.40 2.6 11249 11249_st2 hsa-asg- hsa-asg- 9.52 10.3 10.5 10.140.54 100 9.63 8.99 7.52 9.06 9.73 8.45 8.37 9.19 7.69 8.74 0.79 1001.8E−02 1.40 2.6 5210 5210_st1 hsa-asg- hsa-asg- 11.9 12.0 11.8 11.940.08 100 10.9 11.3 9.81 10.5 11.2 11.0 9.78 10.2 9.91 10.54 0.63 1003.9E−03 1.40 2.6 8552 8552_st2 hsa- hsa- 6.84 6.39 6.49 6.57 0.24 1005.45 6.14 4.68 5.56 4.74 5.63 5.25 4.58 4.54 5.17 0.57 33 2.2E−03 1.402.6 cand283 cand283_st1 hsa-asg- hsa-asg- 6.67 6.97 6.48 6.71 0.25 1004.94 6.29 4.98 5.02 5.03 6.19 4.82 4.96 5.60 5.31 0.57 22 2.4E−03 1.392.6 2696 2696_st1 hsa-asg- hsa-asg- 6.00 5.30 5.48 5.60 0.36 100 3.764.86 3.23 3.28 4.74 4.89 3.93 4.52 4.60 4.20 0.66 0 6.8E−03 1.39 2.63296 3296_st1 hsa-asg- hsa-asg- 7.40 7.38 7.26 7.35 0.07 100 5.65 6.715.39 5.64 5.61 6.60 5.05 6.34 6.59 5.95 0.61 44 3.3E−03 1.39 2.6 49714971_st1 hsa-asg- hsa-asg- 10.1 10.0 9.80 10.01 0.18 100 8.64 9.48 8.148.07 8.63 9.57 7.89 8.69 8.47 8.62 0.59 100 2.8E−03 1.39 2.6 1152811528_st1 hsa- hsa- 6.24 6.96 6.69 6.63 0.36 100 5.68 5.76 5.30 5.175.43 5.49 4.73 4.85 4.76 5.24 0.39 11 2.9E−04 1.39 2.6 miR165 miR165_st1hsa- hsa- 7.03 7.62 6.98 7.21 0.35 100 6.49 6.74 5.47 5.96 6.52 6.434.73 5.76 4.28 5.82 0.86 44 2.3E−02 1.39 2.6 cand855 cand855_st1hsa-asg- hsa-asg- 7.11 8.00 7.96 7.69 0.50 100 6.52 6.97 5.47 6.19 6.986.96 5.92 5.85 5.82 6.30 0.58 67 4.1E−03 1.39 2.6 7388 7388_st1 hsa-asg-hsa-asg- 8.72 8.68 8.35 8.58 0.21 100 7.89 7.78 6.94 7.02 7.43 7.51 6.497.43 6.26 7.19 0.56 100 2.2E−03 1.39 2.6 6203 6203_st2 hsa- hsa- 5.235.59 5.28 5.37 0.19 0 3.56 5.61 2.86 3.46 4.21 4.60 3.29 3.64 4.60 3.980.85 11 2.1E−02 1.39 2.6 cand703 cand703_st2 hsa-asg- hsa-asg- 7.14 7.136.67 6.98 0.27 100 5.14 6.39 5.19 5.23 6.53 5.65 4.90 5.32 6.00 5.600.58 33 3.0E−03 1.39 2.6 10259 10259_st1 hsa-asg- hsa-asg- 6.57 6.486.29 6.45 0.14 100 4.85 6.30 3.73 4.79 5.12 6.09 3.85 5.37 5.44 5.060.88 22 2.5E−02 1.39 2.6 11895 11895_st1 hsa-asg- hsa-asg- 5.14 3.914.46 4.50 0.62 33 4.17 3.64 2.86 2.69 3.54 2.69 3.40 2.78 2.33 3.12 0.590 5.9E−03 1.38 2.6 11443 11443_st1 hsa-asg- hsa-asg- 10.0 10.5 10.310.32 0.24 100 8.91 9.87 8.07 8.69 9.41 9.19 8.34 9.08 8.85 8.94 0.54 781.9E−03 1.38 2.6 8639 8639_st2 hsa-asg- hsa-asg- 4.47 5.77 4.64 4.960.71 33 3.66 4.08 3.54 3.01 3.68 4.24 3.77 2.45 3.77 3.58 0.55 0 5.2E−031.38 2.6 1127 1127_st2 hsa- hsa- 6.22 6.62 6.11 6.32 0.27 33 4.94 5.994.50 5.23 5.03 5.69 4.00 4.18 4.87 4.94 0.65 0 6.0E−03 1.38 2.6 miR166miR166_st1 hsa-miR- hsa-miR- 6.68 5.80 6.25 6.24 0.44 67 4.66 5.28 4.725.06 4.72 5.40 4.33 4.49 5.12 4.86 0.37 0 2.9E−04 1.38 2.6 370 370_st1hsa-asg- hsa-asg- 6.00 5.85 5.63 5.83 0.19 100 4.76 4.95 3.54 3.95 4.005.27 3.93 4.07 5.60 4.45 0.71 0 9.1E−03 1.37 2.6 12094 12094_st1hsa-asg- hsa-asg- 8.26 8.82 8.27 8.45 0.32 100 7.39 7.90 6.27 6.53 7.107.59 6.54 6.96 7.49 7.08 0.55 100 2.7E−03 1.36 2.6 5691 5691_st2hsa-asg- hsa-asg- 8.53 8.80 8.60 8.64 0.14 100 6.86 8.23 6.29 6.39 7.758.21 6.43 7.56 7.79 7.28 0.79 100 1.6E−02 1.36 2.6 8922 8922_st2hsa-asg- hsa-asg- 6.43 6.61 6.21 6.42 0.20 67 5.65 5.51 3.31 4.93 5.495.24 4.78 5.31 5.29 5.05 0.71 0 9.7E−03 1.36 2.6 13661 13661_st1hsa-asg- hsa-asg- 6.30 6.79 6.30 6.46 0.28 100 5.29 6.01 3.73 5.42 5.806.00 4.33 5.13 4.20 5.10 0.83 22 2.2E−02 1.36 2.6 7222 7222_st2 hsa-asg-hsa-asg- 7.06 7.40 6.55 7.00 0.43 100 5.68 6.23 4.72 5.40 6.66 6.03 4.695.85 5.55 5.64 0.65 11 7.7E−03 1.36 2.6 9287 9287_st2 hsa-asg- hsa-asg-6.23 5.81 6.24 6.09 0.25 100 5.92 4.49 5.04 6.08 5.09 4.87 4.14 2.934.07 4.73 0.97 22 4.2E−02 1.36 2.6 8411 8411_st2 hsa-asg- hsa-asg- 5.315.72 5.09 5.37 0.32 33 3.85 4.61 3.39 3.34 4.39 5.37 2.78 4.63 3.77 4.010.80 0 1.9E−02 1.36 2.6 2267 2267_st2 hsa-asg- hsa-asg- 6.92 7.10 6.906.97 0.11 100 5.96 6.71 5.04 5.71 6.48 6.04 4.73 5.22 4.67 5.62 0.74 331.2E−02 1.36 2.6 7579 7579_st2 hsa-asg- hsa-asg- 8.38 9.11 8.45 8.650.41 33 7.03 8.09 6.03 7.13 8.02 7.82 6.78 7.23 7.50 7.29 0.65 447.8E−03 1.35 2.6 747 747_st1 hsa-asg- hsa-asg- 6.33 6.41 6.73 6.49 0.2133 5.29 6.11 4.26 4.66 5.30 5.69 4.73 5.13 5.07 5.14 0.56 0 2.5E−03 1.352.6 9615 9615_st1 hsa-asg- hsa-asg- 6.89 7.28 7.25 7.14 0.22 100 5.896.69 4.75 5.65 6.28 6.55 4.82 5.72 5.75 5.79 0.68 44 8.0E−03 1.35 2.64155 4155_st1 hsa-miR- hsa-miR- 6.23 6.69 6.88 6.60 0.33 100 4.85 6.424.68 5.73 5.61 6.08 3.40 4.41 6.12 5.25 0.99 22 4.7E−02 1.35 2.5 198198_st1 hsa-asg- hsa-asg- 8.73 8.17 8.59 8.50 0.29 100 7.64 7.18 7.348.22 8.03 6.58 6.97 5.74 6.63 7.15 0.78 89 1.6E−02 1.35 2.5 1088310883_st1 hsa-asg- hsa-asg- 6.70 7.04 6.44 6.73 0.30 100 5.14 5.92 5.394.60 4.99 5.74 4.54 5.76 6.36 5.38 0.62 0 5.1E−03 1.35 2.5 1421714217_st1 hsa-asg- hsa-asg- 5.58 5.53 5.26 5.46 0.17 33 4.71 4.81 3.793.34 4.99 5.05 2.32 3.83 4.16 4.11 0.90 0 3.2E−02 1.35 2.5 6631 6631_st1hsa-asg- hsa-asg- 5.99 6.86 6.18 6.34 0.46 100 5.68 4.95 4.81 5.30 5.335.13 5.12 4.78 3.87 5.00 0.51 11 2.3E−03 1.34 2.5 1399 1399_st1 hsa-asg-hsa-asg- 8.15 8.47 8.41 8.34 0.17 100 7.34 7.49 5.55 6.66 7.58 7.52 6.537.84 6.49 7.00 0.74 89 1.2E−02 1.34 2.5 9132 9132_st2 hsa-asg- hsa-asg-8.96 9.32 9.29 9.19 0.20 100 7.91 8.53 7.12 7.77 8.30 8.52 7.07 7.907.49 7.85 0.55 100 2.3E−03 1.34 2.5 4027 4027_st2 hsa-asg- hsa-asg- 7.857.75 7.82 7.81 0.05 100 6.71 7.09 5.14 6.71 7.27 7.19 6.33 6.32 5.426.47 0.75 78 1.3E−02 1.34 2.5 508 508_st2 hsa-asg- hsa-asg- 7.69 7.407.24 7.45 0.23 100 5.85 7.33 4.90 6.19 5.87 6.98 5.29 6.15 6.38 6.100.76 56 1.5E−02 1.34 2.5 8999 8999_st1 hsa-asg- hsa-asg- 6.12 6.90 6.836.62 0.43 67 6.39 5.28 4.17 5.28 5.85 5.09 5.16 5.58 4.70 5.28 0.64 117.5E−03 1.34 2.5 7578 7578_st2 hsa-asg-95 hsa-asg- 7.86 8.21 7.75 7.940.24 100 6.39 7.48 5.72 6.48 6.48 7.23 6.16 6.58 6.87 6.60 0.53 892.1E−03 1.34 2.5 95_st2 hsa-asg- hsa-asg- 6.19 6.59 6.52 6.43 0.21 1004.17 6.62 3.91 4.95 5.07 6.11 4.33 4.96 5.72 5.09 0.91 22 3.4E−02 1.342.5 7439 7439_st1 hsa-asg- hsa-asg- 7.95 8.44 8.37 8.25 0.27 100 6.427.87 6.44 6.54 7.36 7.78 6.16 6.58 7.10 6.92 0.63 100 6.0E−03 1.34 2.511619 11619_st1 hsa-asg- hsa-asg- 8.40 9.00 8.50 8.63 0.32 100 6.97 8.485.97 7.02 7.70 8.05 6.58 7.35 7.57 7.30 0.76 89 1.6E−02 1.33 2.5 1041410414_st1 hsa-asg- hsa-asg- 10.8 10.7 10.4 10.70 0.18 100 10.1 10.1 8.979.23 9.89 9.97 8.64 9.33 7.88 9.36 0.78 89 1.7E−02 1.33 2.5 90929092_st2 hsa-asg- hsa-asg- 9.06 9.42 8.93 9.14 0.25 100 8.09 8.51 6.687.80 8.73 8.79 7.24 7.54 6.83 7.80 0.79 100 1.9E−02 1.33 2.5 777 777_st1hsa-asg- hsa-asg- 8.30 8.52 8.52 8.45 0.12 100 6.79 8.29 6.40 6.69 7.407.74 6.63 6.74 7.36 7.11 0.62 100 5.0E−03 1.33 2.5 12406 12406_st1hsa-asg- hsa-asg- 8.09 8.70 8.74 8.51 0.37 100 7.22 7.75 5.94 7.29 7.777.85 6.57 7.31 6.91 7.18 0.63 89 6.5E−03 1.33 2.5 1919 1919_st1 hsa-asg-hsa-asg- 8.66 9.01 8.83 8.83 0.18 33 8.14 8.40 6.47 7.60 8.06 8.11 6.617.14 6.99 7.50 0.72 56 1.1E−02 1.33 2.5 11155 11155_st1 hsa-asg-hsa-asg- 8.99 9.21 8.77 8.99 0.22 100 7.52 8.66 7.20 7.14 7.79 8.70 7.127.77 7.02 7.66 0.64 100 6.5E−03 1.33 2.5 12946 12946_st1 hsa-asg-hsa-asg- 9.07 9.59 9.33 9.33 0.26 100 7.80 8.87 6.56 7.65 8.92 8.91 7.487.87 7.92 8.00 0.79 100 1.9E−02 1.33 2.5 12001 12001_st1 hsa-asg-hsa-asg- 6.72 6.68 6.62 6.68 0.05 100 5.32 6.14 4.81 5.78 5.15 5.76 4.735.42 5.00 5.35 0.48 11 9.0E−04 1.33 2.5 7215 7215_st2 hsa-asg- hsa-asg-5.62 6.42 5.96 6.00 0.40 100 4.99 5.37 4.02 4.33 5.53 5.60 4.07 4.523.65 4.67 0.72 0 1.3E−02 1.33 2.5 1172 1172_st2 hsa-asg- hsa-asg- 10.710.7 10.6 10.69 0.09 100 9.99 10.1 8.07 9.48 10.2 10.1 8.72 9.38 8.149.36 0.86 89 2.7E−02 1.33 2.5 12136 12136_st2 hsa-asg- hsa-asg- 8.078.25 7.86 8.06 0.19 0 6.73 8.24 6.39 6.13 6.86 7.69 5.58 6.99 6.02 6.740.84 11 2.4E−02 1.33 2.5 4026 4026_st1 hsa-asg- hsa-asg- 5.62 6.15 5.675.82 0.29 100 4.24 4.66 5.09 4.81 4.14 4.84 3.77 4.63 4.24 4.49 0.42 05.3E−04 1.32 2.5 8682 8682_st2 hsa-asg- hsa-asg- 10.1 10.4 10.1 10.260.20 100 9.13 9.56 9.24 8.61 8.88 9.05 8.30 9.01 8.70 8.94 0.37 1001.8E−04 1.32 2.5 3722 3722_st1 hsa-asg- hsa-asg- 9.29 9.50 9.40 9.400.10 100 8.45 8.97 6.52 8.09 8.61 8.95 7.37 8.26 7.55 8.08 0.81 1002.1E−02 1.32 2.5 7653 7653_st1 hsa-asg- hsa-asg- 7.27 7.77 7.12 7.390.34 100 6.06 7.05 5.39 5.68 6.20 6.89 5.16 6.81 5.40 6.07 0.72 561.3E−02 1.31 2.5 5289 5289_st2 hsa-asg- hsa-asg- 4.42 5.63 5.91 5.320.79 33 4.02 4.30 3.06 4.12 4.60 4.37 3.68 4.14 3.77 4.01 0.46 0 4.5E−031.31 2.5 12515 12515_st2 hsa-asg- hsa-asg- 8.17 8.30 7.81 8.09 0.25 1006.92 7.64 6.17 6.39 6.66 7.78 5.75 7.30 6.41 6.78 0.69 100 1.0E−02 1.312.5 12775 12775_st1 hsa-asg- hsa-asg- 7.66 7.77 7.85 7.76 0.10 100 6.257.11 5.24 6.42 7.23 7.22 5.46 6.60 6.51 6.45 0.72 78 1.2E−02 1.31 2.58257 8257_st1 hsa-asg- hsa-asg- 7.50 8.33 7.25 7.69 0.57 100 6.98 7.385.91 6.12 7.07 7.18 5.73 6.04 5.07 6.39 0.79 78 2.6E−02 1.31 2.5 93439343_st1 hsa-asg- hsa-asg- 6.01 6.66 6.38 6.35 0.33 67 5.22 5.78 4.344.93 5.32 5.60 4.00 4.76 5.44 5.04 0.59 11 5.0E−03 1.31 2.5 72787278_st1 hsa-asg- hsa-asg- 6.03 6.17 6.10 6.10 0.07 0 5.07 5.81 3.604.93 4.24 5.46 4.33 4.03 5.64 4.79 0.77 11 1.8E−02 1.31 2.5 70487048_st1 hsa-asg- hsa-asg- 8.67 9.44 8.90 9.00 0.39 100 7.30 8.65 6.707.47 8.17 8.59 7.08 7.39 7.95 7.70 0.68 100 1.1E−02 1.30 2.5 1229712297_st2 hsa-asg- hsa-asg- 9.05 9.54 9.52 9.37 0.27 100 8.18 9.05 6.897.97 8.57 8.41 7.62 8.36 7.56 8.07 0.64 100 7.6E−03 1.30 2.5 91859185_st2 hsa-asg- hsa-asg- 7.13 7.77 7.27 7.39 0.34 100 5.87 6.96 5.265.94 6.13 6.89 5.12 6.10 6.52 6.09 0.64 44 8.0E−03 1.30 2.5 67926792_st1 hsa-asg- hsa-asg- 9.89 10.1 9.82 9.94 0.15 100 8.67 9.47 7.478.39 8.77 9.28 7.92 8.72 9.05 8.64 0.64 44 6.7E−03 1.30 2.5 39523952_st1 hsa-asg- hsa-asg- 8.46 8.50 8.25 8.41 0.13 100 6.37 7.98 5.826.66 7.06 8.09 6.59 7.29 8.09 7.10 0.82 89 2.4E−02 1.30 2.5 35843584_st2 hsa-asg- hsa-asg- 7.11 7.43 6.69 7.08 0.37 100 4.90 6.96 5.515.47 5.75 6.74 4.69 5.87 6.13 5.78 0.76 22 1.8E−02 1.30 2.5 45234523_st2 hsa-asg- hsa-asg- 8.59 8.73 8.14 8.49 0.31 100 7.07 7.80 6.076.85 7.25 8.08 7.31 7.31 6.95 7.19 0.57 89 4.3E−03 1.30 2.5 17751775_st2 hsa-miR- hsa-miR- 10.2 10.8 11.2 10.77 0.48 100 9.78 9.28 9.209.98 9.13 9.06 9.64 9.52 9.66 9.47 0.32 100 2.8E−04 1.29 2.5 652 652_st1hsa-asg- hsa-asg- 8.71 6.84 7.76 7.77 0.94 100 7.43 6.36 6.67 7.04 6.266.27 6.63 6.55 5.09 6.48 0.64 89 2.1E−02 1.29 2.5 9696 9696_st1 hsa-asg-hsa-asg- 11.0 11.2 11.1 11.13 0.09 100 10.1 10.6 8.73 9.77 10.4 10.79.17 9.97 8.89 9.84 0.75 100 1.6E−02 1.29 2.4 9077 9077_st2 hsa-asg-hsa-asg- 6.16 6.47 6.68 6.44 0.26 33 5.32 6.01 4.72 5.53 4.45 5.76 4.904.71 4.92 5.15 0.53 0 2.7E−03 1.29 2.4 13047 13047_st2 hsa-miR- hsa-miR-6.90 7.42 7.57 7.30 0.35 100 5.39 6.35 5.35 5.81 6.39 6.64 5.25 5.807.06 6.01 0.64 44 8.3E−03 1.29 2.4 564 564_st2 hsa-asg- hsa-asg- 5.716.06 5.63 5.80 0.23 100 3.76 5.43 3.23 4.69 4.85 5.81 3.29 4.78 4.734.51 0.90 0 3.8E−02 1.29 2.4 9735 9735_st1 hsa-miR- hsa-miR- 6.91 7.677.55 7.38 0.41 100 6.42 6.72 6.06 6.22 5.80 6.66 5.59 5.77 5.53 6.090.45 100 1.3E−03 1.29 2.4 142-5p 142-5p_st2 hsa-asg- hsa-asg- 6.07 6.015.89 5.99 0.09 100 5.29 4.55 4.61 4.26 5.07 4.80 4.38 4.87 4.47 4.700.33 0 7.7E−05 1.29 2.4 12512 12512_st1 hsa- hsa- 4.37 4.79 4.93 4.700.29 67 4.61 4.43 2.66 3.40 2.69 2.69 2.78 3.20 4.24 3.41 0.81 0 2.4E−021.29 2.4 miR144 miR144_st2 hsa-asg- hsa-asg- 7.94 8.17 7.92 8.01 0.14100 6.30 7.92 6.29 6.53 6.76 8.07 5.84 6.21 6.61 6.73 0.77 100 1.8E−021.28 2.4 8633 8633_st1 hsa-asg- hsa-asg- 7.19 7.43 7.17 7.26 0.14 1005.32 7.09 5.21 5.54 5.76 6.62 4.82 6.58 6.87 5.98 0.82 44 2.6E−02 1.282.4 3691 3691_st1 hsa-asg- hsa-asg- 9.68 9.72 9.44 9.61 0.15 100 7.808.89 7.98 8.34 8.50 8.92 7.74 8.39 8.46 8.33 0.43 100 5.7E−04 1.28 2.42739 2739_st2 hsa-asg- hsa-asg- 4.98 5.27 5.38 5.21 0.21 67 3.76 4.553.23 3.77 3.72 4.91 3.93 3.99 3.53 3.93 0.51 0 2.1E−03 1.28 2.4 961961_st1 hsa-asg- hsa-asg- 9.26 9.68 9.15 9.36 0.28 100 8.76 8.57 7.497.93 8.92 8.49 7.38 7.99 7.22 8.08 0.63 100 7.6E−03 1.28 2.4 15731573_st2 hsa-asg- hsa-asg- 9.37 9.31 9.19 9.29 0.09 100 8.57 8.62 7.117.86 8.75 8.42 7.72 7.71 7.32 8.01 0.60 100 5.2E−03 1.28 2.4 32713271_st1 hsa- hsa- 5.42 3.44 4.64 4.50 0.99 33 2.61 4.16 3.67 2.51 3.683.69 2.64 2.78 3.25 3.22 0.60 0 2.0E−02 1.28 2.4 cand207 cand207_st1hsa-asg- hsa-asg- 8.25 8.04 7.92 8.07 0.16 100 6.18 8.26 7.22 6.15 6.377.93 5.69 6.28 7.08 6.79 0.88 78 3.5E−02 1.28 2.4 6180 6180_st1 hsa-asg-hsa-asg- 9.34 9.44 9.28 9.35 0.08 67 8.13 9.14 7.25 7.72 7.94 9.30 7.328.58 7.32 8.08 0.78 11 2.1E−02 1.27 2.4 7753 7753_st2 hsa-asg- hsa-asg-10.2 10.5 10.2 10.34 0.21 100 9.03 9.93 7.79 8.92 10.18 9.57 8.51 9.288.41 9.07 0.76 100 1.9E−02 1.27 2.4 11946 11946_st1 hsa-asg- hsa-asg-7.57 8.12 7.64 7.78 0.30 100 7.46 6.92 6.58 7.00 6.90 6.76 6.73 5.934.24 6.50 0.94 89 4.8E−02 1.27 2.4 14170 14170_st1 hsa-asg- hsa-asg-7.11 7.23 7.33 7.22 0.11 100 5.78 7.19 4.30 5.87 5.76 6.72 5.29 5.846.80 5.95 0.87 78 3.4E−02 1.27 2.4 4262 4262_st1 hsa-asg- hsa-asg- 7.627.56 7.19 7.46 0.24 100 5.92 7.17 4.90 6.10 6.96 6.72 5.43 6.42 6.066.19 0.73 67 1.6E−02 1.27 2.4 6189 6189_st2 hsa-miR- hsa-miR- 9.54 10.410.9 10.31 0.71 100 8.65 9.16 8.27 9.95 9.26 8.92 9.78 9.34 7.98 9.030.65 100 1.6E−02 1.27 2.4 486 486_st2 hsa-asg- hsa-asg- 10.1 10.3 9.9810.14 0.16 100 8.55 9.77 8.46 8.38 9.02 9.64 8.09 9.63 8.28 8.87 0.66100 9.0E−03 1.27 2.4 8163 8163_st1 hsa-asg- hsa-asg- 8.06 8.15 7.90 8.040.13 100 7.21 7.35 6.05 6.92 6.85 7.38 6.24 6.88 6.03 6.77 0.53 892.6E−03 1.27 2.4 12286 12286_st2 hsa-asg- hsa-asg- 9.70 10.1 9.67 9.830.25 100 8.51 9.23 7.30 8.59 9.51 9.36 7.83 8.48 8.25 8.56 0.73 221.6E−02 1.27 2.4 7044 7044_st1 hsa-asg- hsa-asg- 7.03 7.51 7.19 7.240.25 100 6.45 6.78 4.17 5.95 6.61 6.78 5.29 6.15 5.58 5.97 0.86 673.3E−02 1.27 2.4 3945 3945_st2 hsa-asg- hsa-asg- 7.65 7.67 7.48 7.600.10 100 6.59 7.20 5.58 5.78 6.90 6.78 5.51 6.78 5.82 6.33 0.65 568.2E−03 1.27 2.4 12424 12424_st2 hsa-asg- hsa-asg- 7.27 7.64 7.37 7.430.19 100 5.85 6.91 5.43 5.54 6.24 6.71 5.66 6.62 6.47 6.16 0.55 673.5E−03 1.27 2.4 8636 8636_st2 hsa-asg- hsa-asg- 7.87 8.21 7.76 7.950.23 100 7.13 7.55 5.09 6.57 7.74 7.29 6.05 6.60 6.09 6.68 0.85 893.2E−02 1.27 2.4 13041 13041_st1 hsa-asg- hsa-asg- 7.02 7.47 7.29 7.260.22 100 6.68 6.40 5.82 6.62 6.67 5.74 5.32 5.31 5.40 6.00 0.60 335.8E−03 1.27 2.4 8067 8067_st2 hsa-miR- hsa-miR- 7.79 8.13 7.23 7.720.45 0 6.20 7.38 4.98 6.22 7.00 6.98 5.50 6.57 7.24 6.45 0.81 11 3.0E−021.26 2.4 483 483-_st1 hsa-asg- hsa-asg- 8.68 9.07 8.61 8.79 0.25 1007.20 8.61 6.24 7.12 8.36 7.86 6.94 7.43 7.93 7.52 0.74 89 1.8E−02 1.262.4 4990 4990_st2 hsa-cand6 hsa- 7.96 8.42 8.19 8.19 0.23 100 7.04 7.846.10 6.91 7.20 7.41 6.29 6.45 7.13 6.93 0.56 89 4.1E−03 1.26 2.4cand6_st2 hsa-asg- hsa-asg- 8.54 8.81 8.58 8.64 0.15 0 7.92 8.13 6.517.17 7.90 7.81 6.76 7.38 6.87 7.38 0.59 44 5.0E−03 1.26 2.4 471 471_st1hsa-asg- hsa-asg- 7.70 7.96 7.35 7.67 0.31 100 6.32 7.55 5.82 6.03 6.447.08 5.05 6.69 6.72 6.41 0.73 67 1.8E−02 1.26 2.4 468 468_st1 hsa-asg-hsa-asg- 7.06 7.41 7.33 7.27 0.18 100 5.07 6.85 6.22 5.69 6.28 6.86 5.596.34 5.20 6.01 0.66 33 9.9E−03 1.26 2.4 3112 3112_st1 hsa- hsa- 6.326.84 6.58 6.58 0.26 100 5.45 6.05 5.47 3.87 5.38 6.06 4.69 5.76 5.205.32 0.69 22 1.3E−02 1.26 2.4 cand224 cand224_st1 hsa-asg- hsa-asg- 7.257.71 7.56 7.51 0.24 100 6.40 7.11 5.62 6.29 6.84 7.07 5.12 5.89 5.936.25 0.68 56 1.2E−02 1.26 2.4 887 887_st2 hsa-asg- hsa-asg- 10.3 10.710.8 10.66 0.24 100 9.91 10.2 8.44 9.65 10.3 9.93 8.80 9.41 7.96 9.410.83 100 3.1E−02 1.26 2.4 4731 4731_st1 hsa-asg- hsa-asg- 4.42 5.03 4.374.61 0.37 33 3.45 3.92 3.23 3.28 2.83 4.43 3.29 3.44 2.33 3.35 0.60 07.2E−03 1.25 2.4 2441 2441_st1 hsa-asg- hsa-asg- 9.02 9.73 9.11 9.290.39 33 8.14 8.82 6.98 8.08 8.63 8.69 7.15 8.00 7.80 8.03 0.65 0 1.1E−021.25 2.4 9003 9003_st2 hsa-miR- hsa-miR- 4.61 3.72 3.39 3.91 0.63 333.21 2.71 3.23 1.93 2.69 2.85 2.48 2.08 2.74 2.66 0.44 0 3.1E−03 1.252.4 520h 520h_st1 hsa-asg- hsa-asg- 8.87 8.93 8.54 8.78 0.21 100 7.808.50 6.59 7.27 7.48 8.06 6.95 7.72 7.38 7.53 0.57 100 4.9E−03 1.25 2.4819 819_st1 hsa-asg- hsa-asg- 5.66 4.51 5.12 5.10 0.57 67 4.31 3.74 4.394.58 3.33 3.48 2.78 3.95 4.07 3.85 0.57 0 8.4E−03 1.25 2.4 1423014230_st1 hsa-asg- hsa-asg- 8.19 8.69 8.43 8.44 0.25 100 7.37 8.05 6.227.22 7.51 7.76 6.46 7.07 7.03 7.19 0.58 100 5.5E−03 1.25 2.4 11741174_st2 hsa-asg- hsa-asg- 8.92 9.35 8.96 9.07 0.24 100 8.10 8.33 6.997.49 8.30 7.92 6.92 8.10 8.30 7.83 0.56 100 4.3E−03 1.25 2.4 33453345_st1 hsa-asg- hsa-asg- 7.56 7.95 7.63 7.71 0.21 100 5.83 7.51 5.825.63 6.63 7.14 5.61 6.98 7.06 6.47 0.75 67 1.9E−02 1.24 2.4 32453245_st1 hsa-asg- hsa-asg- 4.98 5.97 4.86 5.27 0.61 33 3.76 4.95 4.023.08 4.27 4.65 2.64 4.28 4.60 4.03 0.76 0 2.8E−02 1.24 2.4 3003 3003_st1hsa-asg-47 hsa-asg- 8.68 8.94 8.64 8.75 0.16 100 7.27 8.31 7.18 7.257.66 8.14 6.75 7.56 7.50 7.51 0.48 100 1.7E−03 1.24 2.4 47_st1 hsa- hsa-11.3 11.4 11.0 11.29 0.19 100 10.5 10.9 9.27 10.0 10.4 10.7 9.19 9.969.25 10.05 0.68 100 1.2E−02 1.24 2.4 cand110 cand110_st2 hsa-asg-hsa-asg- 10.7 10.8 9.93 10.53 0.52 100 10.2 10.2 8.59 9.21 9.68 10.08.47 9.08 8.15 9.29 0.78 89 2.9E−02 1.24 2.4 11514 11514_st1 hsa-asg-hsa-asg- 8.83 8.98 9.43 9.08 0.32 100 8.26 8.50 7.19 7.22 8.78 7.90 7.057.92 7.76 7.84 0.61 100 7.6E−03 1.24 2.4 4059 4059_st1 hsa-asg- hsa-asg-6.36 7.43 7.24 7.01 0.57 67 6.06 6.25 5.01 6.11 6.26 6.10 5.12 5.60 5.445.77 0.49 11 4.3E−03 1.24 2.4 7240 7240_st1 hsa-asg- hsa-asg- 7.64 7.807.39 7.61 0.21 100 5.29 7.36 6.01 5.90 6.56 7.65 5.16 6.79 6.66 6.380.86 67 3.7E−02 1.24 2.4 12949 12949_st1 hsa-asg- hsa-asg- 9.24 9.109.02 9.12 0.11 100 7.80 8.72 7.48 7.27 8.12 8.43 7.13 8.14 7.87 7.880.53 100 3.0E−03 1.23 2.4 5123 5123_st1 hsa-asg- hsa-asg- 8.56 9.09 8.668.77 0.28 100 7.37 8.43 6.31 7.42 8.05 8.58 6.96 7.71 6.99 7.54 0.74 892.0E−02 1.23 2.3 8460 8460_st2 hsa-asg- hsa-asg- 9.38 9.70 9.46 9.510.17 100 8.18 9.04 6.93 8.28 8.92 8.91 7.81 8.38 8.08 8.28 0.66 1001.1E−02 1.23 2.3 6802 6802_st1 hsa-asg- hsa-asg- 6.66 6.87 6.65 6.730.13 100 5.62 6.69 3.96 5.21 5.29 6.31 4.73 5.49 6.17 5.50 0.84 333.4E−02 1.23 2.3 11797 11797_st1 hsa-asg- hsa-asg- 8.68 8.77 8.70 8.720.05 100 7.32 8.36 6.79 7.06 7.23 7.98 7.08 7.70 7.89 7.49 0.52 1002.5E−03 1.23 2.3 11228 11228_st1 hsa-asg- hsa-asg- 8.10 8.45 7.95 8.170.25 100 8.14 7.38 6.22 6.59 7.32 7.26 6.39 7.19 5.96 6.94 0.69 1001.5E−02 1.23 2.3 7173 7173_st1 hsa-asg- hsa-asg- 5.86 6.55 6.39 6.270.36 100 5.51 6.04 3.79 5.16 5.24 5.70 4.00 5.19 4.73 5.04 0.75 222.3E−02 1.23 2.3 9321 9321_st1 hsa-miR- hsa-miR- 10.2 10.6 10.4 10.450.20 100 9.10 9.02 9.17 9.80 8.98 8.85 9.69 9.24 9.14 9.22 0.32 1001.0E−04 1.23 2.3 422a 422a_st1 hsa-asg- hsa-asg- 5.52 5.72 5.22 5.490.25 0 3.93 5.81 4.07 2.93 4.18 4.93 3.68 4.11 4.70 4.26 0.81 11 3.1E−021.23 2.3 4783 4783_st2 hsa-asg- hsa-asg- 8.08 7.99 7.70 7.93 0.20 1007.35 7.48 6.46 6.04 6.83 7.46 5.80 6.65 6.24 6.70 0.63 100 9.0E−03 1.232.3 688 688_st2 hsa-asg- hsa-asg- 9.78 9.65 9.75 9.73 0.07 100 8.82 9.327.62 8.26 8.82 9.12 7.64 8.80 8.11 8.50 0.62 22 7.9E−03 1.22 2.3 36643664_st1 hsa-asg- hsa-asg- 5.58 5.78 5.06 5.47 0.37 100 4.37 5.04 4.123.52 4.79 5.05 3.17 4.96 3.25 4.25 0.77 0 2.7E−02 1.22 2.3 1897 1897_st1hsa-asg- hsa-asg- 6.37 6.71 6.41 6.50 0.19 100 5.51 6.34 4.30 5.30 5.925.34 4.69 5.15 4.95 5.28 0.62 33 8.1E−03 1.22 2.3 551 551_st2 hsa-asg-hsa-asg- 5.86 5.80 5.15 5.60 0.40 67 4.02 5.72 3.54 3.21 4.45 4.97 3.855.22 4.47 4.38 0.82 11 3.5E−02 1.22 2.3 5228 5228_st2 hsa-asg- hsa-asg-9.49 9.81 9.51 9.60 0.18 100 8.44 8.69 7.34 8.32 9.34 8.54 7.91 9.267.65 8.39 0.68 100 1.3E−02 1.22 2.3 8421 8421_st1 hsa-asg- hsa-asg- 6.197.05 7.01 6.75 0.49 100 5.39 6.14 4.98 4.97 5.77 6.20 5.16 5.79 5.395.53 0.47 33 3.0E−03 1.22 2.3 3073 3073_st2 hsa-asg- hsa-asg- 8.56 9.278.63 8.82 0.39 100 7.87 8.27 6.05 7.60 8.53 8.12 6.87 7.51 7.61 7.600.76 89 2.6E−02 1.22 2.3 6021 6021_st2 hsa-asg- hsa-asg- 10.8 11.1 10.810.94 0.18 100 10.1 10.2 8.94 9.79 10.6 10.1 9.14 9.69 8.74 9.73 0.66100 1.2E−02 1.22 2.3 9503 9503_st2 hsa-asg- hsa-asg- 9.33 9.57 9.37 9.420.13 100 8.30 9.09 7.03 8.19 9.41 8.61 7.84 8.42 6.99 8.21 0.82 03.3E−02 1.21 2.3 746 746_st1 hsa-asg- hsa-asg- 7.61 7.87 7.83 7.77 0.14100 6.75 7.11 5.82 6.30 7.28 7.02 6.32 6.42 5.98 6.56 0.51 89 2.7E−031.21 2.3 5265 5265_st1 hsa-asg- hsa-asg- 9.86 10.3 9.98 10.07 0.26 1009.10 9.16 7.65 8.74 10.0 8.97 8.52 8.97 8.56 8.86 0.64 100 1.0E−02 1.212.3 1637 1637_st2 hsa-asg- hsa-asg- 8.81 9.40 8.96 9.06 0.31 100 7.728.58 6.29 7.51 8.98 8.34 7.01 7.98 8.18 7.84 0.82 89 3.5E−02 1.21 2.33167 3167_st1 hsa-asg- hsa-asg- 9.49 9.94 9.72 9.72 0.23 100 8.85 9.327.37 8.79 8.92 9.11 7.91 8.46 7.82 8.51 0.66 100 1.2E−02 1.21 2.3 504504_st2 hsa-asg- hsa-asg- 5.14 5.77 5.55 5.48 0.32 67 3.93 5.61 3.912.69 4.08 5.37 3.93 4.28 4.67 4.27 0.87 11 4.4E−02 1.21 2.3 1148311483_st1 hsa-asg- hsa-asg- 9.06 9.30 8.98 9.11 0.16 100 7.58 8.47 6.907.20 8.50 8.08 7.36 8.60 8.44 7.90 0.65 100 1.1E−02 1.21 2.3 90049004_st1 hsa-asg- hsa-asg- 9.13 9.39 9.12 9.21 0.16 100 8.74 8.73 7.367.60 8.43 8.35 7.14 7.72 7.95 8.00 0.59 100 6.5E−03 1.21 2.3 50325032_st2 hsa-asg- hsa-asg- 7.28 7.80 7.55 7.54 0.26 100 6.42 6.99 5.796.54 6.79 6.89 6.05 5.80 5.74 6.34 0.50 67 2.9E−03 1.21 2.3 11671167_st1 hsa-asg- hsa-asg- 5.71 5.30 4.96 5.32 0.37 67 3.76 4.76 3.674.00 4.14 4.89 3.17 4.52 4.16 4.12 0.55 0 5.8E−03 1.21 2.3 1289312893_st2 hsa-asg- hsa-asg- 8.69 8.77 8.53 8.66 0.12 100 7.55 8.32 6.067.03 8.19 8.10 6.93 7.52 7.43 7.46 0.72 89 1.8E−02 1.20 2.3 99749974_st1 hsa-asg- hsa-asg- 7.79 8.43 8.10 8.10 0.32 0 8.02 7.00 5.746.88 7.99 7.03 6.49 7.25 5.69 6.90 0.83 33 3.8E−02 1.20 2.3 1288012880_st1 hsa-asg- hsa-asg- 9.27 9.70 9.38 9.45 0.22 67 8.52 8.87 6.998.46 9.21 8.75 7.58 8.24 7.58 8.25 0.72 11 1.9E−02 1.20 2.3 33253325_st1 hsa-asg- hsa-asg- 9.76 10.1 10.0 9.97 0.20 100 8.63 8.58 8.709.41 8.77 8.37 9.21 8.76 8.49 8.77 0.34 100 1.9E−04 1.20 2.3 39983998_st1 hsa-asg- hsa-asg- 11.5 11.8 11.3 11.57 0.21 100 10.5 11.0 8.8310.5 11.6 10.8 9.86 10.2 9.76 10.37 0.82 100 3.5E−02 1.20 2.3 68006800_st1 hsa-asg- hsa-asg- 8.45 8.48 8.69 8.54 0.13 0 8.10 7.93 6.727.33 7.72 7.49 6.61 7.79 6.39 7.34 0.62 44 9.3E−03 1.20 2.3 1242112421_st1 hsa-asg- hsa-asg- 7.44 7.57 7.21 7.41 0.18 100 5.94 7.46 4.586.10 6.40 6.99 5.43 6.29 6.65 6.20 0.85 67 4.0E−02 1.20 2.3 73527352_st1 hsa-asg- hsa-asg- 9.38 9.71 9.56 9.55 0.17 100 7.78 9.28 7.437.92 9.11 9.27 7.66 8.02 8.69 8.35 0.74 100 2.1E−02 1.20 2.3 36703670_st1 hsa-asg- hsa-asg- 5.00 4.07 3.79 4.29 0.63 33 4.24 3.16 2.433.68 2.69 2.35 4.14 3.26 1.86 3.09 0.83 0 4.6E−02 1.20 2.3 1511 1511_st1hsa- hsa- 11.1 12.1 12.3 11.90 0.67 100 10.9 10.9 9.74 10.9 11.5 10.410.4 11.5 9.71 10.70 0.67 100 2.3E−02 1.20 2.3 cand421 cand421_st1hsa-asg- hsa-asg- 8.16 8.53 8.39 8.36 0.19 100 6.98 8.05 6.75 7.16 6.997.39 6.19 7.54 7.46 7.17 0.53 100 3.9E−03 1.19 2.3 5115 5115_st1hsa-asg- hsa-asg- 8.83 9.30 8.73 8.96 0.30 100 7.35 8.30 6.68 7.70 8.708.37 7.41 8.00 7.35 7.76 0.64 100 1.1E−02 1.19 2.3 6952 6952_st1hsa-asg- hsa-asg- 5.33 5.94 6.37 5.88 0.52 33 4.85 5.73 4.65 4.40 4.635.24 3.05 4.89 4.76 4.69 0.73 11 2.7E−02 1.19 2.3 2311 2311_st1 hsa-asg-hsa-asg- 6.64 6.86 6.52 6.67 0.17 100 5.35 6.30 3.85 4.97 6.28 5.93 4.905.66 6.13 5.49 0.81 44 3.4E−02 1.19 2.3 12867 12867_st2 hsa-miR-9*hsa-miR-9-_st2 7.82 5.45 7.01 6.76 1.20 100 6.20 6.10 5.57 6.11 5.415.87 4.94 4.73 5.23 5.57 0.54 44 3.2E−02 1.19 2.3 hsa-asg- hsa-asg- 4.655.35 5.31 5.10 0.39 67 4.49 3.83 4.30 4.36 4.39 4.49 2.48 3.64 3.25 3.920.69 0 1.9E−02 1.19 2.3 6352 6352_st2 hsa-asg- hsa-asg- 4.95 5.30 4.905.05 0.22 33 4.81 4.16 2.66 3.01 4.14 4.55 3.29 3.64 4.54 3.87 0.75 02.6E−02 1.18 2.3 8505 8505_st1 hsa-asg- hsa-asg- 9.64 9.40 9.02 9.350.31 33 8.65 8.85 7.59 7.87 8.57 8.56 7.39 8.20 7.82 8.17 0.52 0 4.3E−031.18 2.3 6092 6092_st2 hsa-asg- hsa-asg- 5.39 5.41 5.36 5.39 0.03 674.61 5.04 3.91 4.46 4.60 4.18 2.32 4.21 4.54 4.21 0.78 0 2.8E−02 1.182.3 5157 5157_st1 hsa-asg- hsa-asg- 8.22 8.53 8.52 8.42 0.18 100 7.068.05 5.89 6.98 8.40 7.98 6.11 7.13 7.58 7.24 0.86 89 4.4E−02 1.18 2.31888 1888_st1 hsa-asg- hsa-asg- 5.14 4.63 4.86 4.88 0.26 33 3.66 4.303.91 3.52 3.97 3.69 3.29 3.54 3.39 3.70 0.32 0 1.7E−04 1.18 2.3 1139711397_st1 hsa-asg- hsa-asg- 4.98 5.33 5.06 5.12 0.18 67 3.33 4.95 3.794.00 4.33 4.60 2.78 3.83 3.87 3.94 0.65 0 1.2E−02 1.18 2.3 1917 1917_st1hsa-asg- hsa-asg- 8.30 8.67 8.15 8.37 0.27 100 6.96 7.93 5.99 7.03 7.738.22 6.62 7.13 7.17 7.20 0.68 89 1.7E−02 1.18 2.3 9164 9164_st1 hsa-asg-hsa-asg- 8.27 8.43 7.80 8.17 0.33 0 7.33 7.51 6.23 6.94 7.76 7.34 6.297.64 5.89 6.99 0.69 33 1.9E−02 1.18 2.3 6149 6149_st2 hsa-asg- hsa-asg-5.27 5.92 5.68 5.63 0.33 0 4.66 5.83 3.73 4.19 4.47 4.03 4.14 4.11 4.924.45 0.63 11 1.2E−02 1.17 2.3 5880 5880_st1 hsa-asg- hsa-asg- 7.02 7.126.91 7.02 0.11 100 6.27 6.78 5.30 5.54 6.06 6.13 4.82 6.10 5.61 5.850.59 44 7.5E−03 1.17 2.2 13963 13963_st2 hsa-asg- hsa-asg- 9.55 9.809.66 9.67 0.13 100 8.91 9.13 7.46 8.66 9.81 8.77 8.03 8.53 7.24 8.510.81 100 3.6E−02 1.17 2.2 7450 7450_st1 hsa-asg- hsa-asg- 5.08 5.75 5.465.43 0.33 100 4.31 5.04 2.43 4.30 4.18 4.97 4.07 4.35 4.73 4.26 0.77 113.2E−02 1.17 2.2 12353 12353_st2 hsa-asg- hsa-asg- 9.97 9.98 9.69 9.880.17 100 8.63 9.48 8.34 8.27 8.76 9.60 7.96 9.25 8.14 8.71 0.60 449.2E−03 1.16 2.2 11758 11758_st2 hsa-asg- hsa-asg- 7.60 8.21 8.14 7.980.33 100 6.86 7.66 5.94 6.77 7.39 7.64 5.96 6.77 6.38 6.82 0.65 891.6E−02 1.16 2.2 1641 1641_st1 hsa-asg- hsa-asg- 4.92 3.20 4.11 4.070.86 33 2.43 3.29 3.54 3.28 2.69 1.97 3.50 2.62 2.92 2.92 0.53 0 1.7E−021.16 2.2 9702 9702_st2 hsa-asg- hsa-asg- 6.16 5.65 5.65 5.82 0.30 1004.31 5.58 3.79 4.81 4.00 5.27 3.85 4.52 5.81 4.66 0.75 11 3.0E−02 1.162.2 8444 8444_st2 hsa-asg- hsa-asg- 7.75 6.70 7.26 7.24 0.52 100 5.926.06 6.67 6.74 6.18 6.02 6.12 4.58 6.44 6.08 0.63 78 1.7E−02 1.16 2.211883 11883_st1 hsa-asg- hsa-asg- 8.72 9.35 8.89 8.99 0.32 100 7.69 8.466.52 7.99 9.10 8.38 7.37 7.63 7.36 7.83 0.76 100 3.1E−02 1.16 2.2 74267426_st1 hsa-asg- hsa-asg- 4.86 5.98 5.47 5.44 0.56 0 4.31 5.65 3.143.77 4.30 5.17 3.77 4.03 4.43 4.29 0.75 11 3.7E−02 1.15 2.2 202 202_st1hsa-asg- hsa-asg- 6.50 7.16 6.79 6.81 0.33 100 4.94 5.85 4.78 5.97 6.856.13 5.22 5.43 5.80 5.66 0.64 44 1.5E−02 1.15 2.2 12395 12395_st2hsa-asg- hsa-asg- 7.23 7.61 7.34 7.39 0.20 100 6.48 7.08 5.47 6.18 6.626.90 5.59 6.26 5.61 6.25 0.59 67 8.9E−03 1.15 2.2 10207 10207_st2hsa-asg- hsa-asg- 5.76 6.38 5.61 5.91 0.41 100 5.32 5.30 4.02 4.63 4.854.84 4.49 5.20 4.24 4.77 0.47 44 3.5E−03 1.15 2.2 7437 7437_st2 hsa-asg-hsa-asg- 6.89 7.22 7.08 7.06 0.17 100 6.30 6.61 5.17 5.51 5.89 6.83 5.226.02 5.74 5.92 0.58 33 8.6E−03 1.14 2.2 7294 7294_st2 hsa-asg- hsa-asg-5.08 5.72 4.96 5.25 0.40 100 4.49 4.76 2.86 4.08 4.63 3.95 3.50 4.354.40 4.11 0.61 11 1.3E−02 1.14 2.2 2395 2395_st1 hsa-asg- hsa-asg- 8.619.19 8.94 8.91 0.29 100 7.10 8.48 7.36 7.24 8.12 8.38 7.42 7.84 8.007.77 0.51 100 4.7E−03 1.14 2.2 11617 11617_st2 hsa-asg- hsa-asg- 8.959.23 9.03 9.07 0.15 100 7.83 8.73 6.79 7.67 8.08 8.47 7.39 7.93 8.487.93 0.60 100 1.0E−02 1.14 2.2 3999 3999_st1 hsa-asg- hsa-asg- 6.64 6.876.21 6.57 0.33 67 5.60 6.01 5.21 5.14 5.57 5.49 4.78 5.34 5.80 5.44 0.3711 8.2E−04 1.14 2.2 1689 1689_st2 hsa-asg- hsa-asg- 10.4 10.7 10.5 10.600.15 100 9.80 10.2 8.40 9.53 10.3 10.0 9.02 9.27 8.51 9.46 0.72 1002.4E−02 1.14 2.2 3456 3456_st2 hsa-asg- hsa-asg- 9.03 9.32 8.95 9.100.19 100 8.52 8.48 6.94 7.96 8.83 8.30 7.29 7.92 7.44 7.96 0.63 1001.3E−02 1.14 2.2 2672 2672_st1 hsa-asg- hsa-asg- 7.97 8.12 7.83 7.970.14 0 6.34 7.32 7.49 6.84 6.74 6.98 6.59 6.69 6.55 6.84 0.37 22 5.0E−041.14 2.2 2862 2862_st1 hsa-asg- hsa-asg- 8.02 8.55 7.72 8.09 0.42 1007.53 7.38 6.29 7.00 7.24 7.35 5.78 7.19 6.89 6.96 0.58 100 1.1E−02 1.132.2 10805 10805_st1 hsa-asg- hsa-asg- 8.80 9.37 9.24 9.14 0.30 100 8.158.69 7.14 7.74 8.29 8.32 7.61 8.46 7.68 8.01 0.49 100 4.3E−03 1.13 2.24031 4031_st2 hsa-asg- hsa-asg- 7.56 7.95 7.99 7.83 0.24 100 6.39 8.035.47 6.40 6.79 7.54 6.12 6.57 7.04 6.70 0.76 89 3.3E−02 1.13 2.2 98499849_st2 hsa- hsa- 4.69 3.79 4.11 4.19 0.46 33 2.61 3.53 3.06 3.15 3.333.59 1.79 3.99 2.54 3.07 0.67 0 2.2E−02 1.13 2.2 cand208 cand208_st1hsa-asg- hsa-asg- 7.93 8.03 7.63 7.86 0.21 100 6.08 7.76 5.99 6.58 7.077.32 6.07 6.88 6.83 6.73 0.61 78 1.2E−02 1.13 2.2 7221 7221_st1hsa-asg-88 hsa-asg- 11.0 11.3 11.0 11.14 0.15 100 10.3 10.7 8.78 9.8410.5 10.5 9.41 10.2 9.73 10.02 0.63 100 1.3E−02 1.13 2.2 88_st1 hsa-asg-hsa-asg- 8.43 8.61 8.40 8.48 0.11 100 7.01 8.30 6.55 6.92 8.24 7.76 6.837.19 7.35 7.35 0.62 100 1.2E−02 1.13 2.2 916 916_st1 hsa- hsa- 6.44 6.496.61 6.52 0.09 100 5.57 5.91 4.93 5.19 5.42 5.85 4.64 5.36 5.63 5.390.42 33 1.1E−03 1.13 2.2 cand753 cand753_st1 hsa-asg- hsa-asg- 6.25 6.476.53 6.42 0.15 33 5.39 6.40 4.34 5.64 5.24 5.38 4.14 4.85 6.24 5.29 0.770 3.3E−02 1.13 2.2 2563 2563_st2 hsa- hsa- 10.1 9.98 9.66 9.92 0.23 1009.18 9.26 8.63 8.62 9.36 9.32 8.07 8.59 8.09 8.79 0.51 44 4.7E−03 1.122.2 cand756 cand756_st1 hsa-asg- hsa-asg- 9.16 9.32 9.22 9.23 0.08 1007.97 8.85 6.83 8.01 8.70 8.51 7.51 8.48 8.12 8.11 0.64 100 1.4E−02 1.122.2 10716 10716_st1 hsa-asg- hsa-asg- 8.40 8.43 8.33 8.39 0.05 100 7.607.86 6.78 7.34 7.28 7.63 7.01 7.31 6.56 7.26 0.42 100 1.1E−03 1.12 2.27617 7617_st1 hsa-asg- hsa-asg- 10.8 10.8 10.6 10.78 0.14 100 10.3 10.48.97 9.73 10.2 10.4 9.07 9.28 8.35 9.66 0.77 100 3.4E−02 1.12 2.2 70967096_st2 hsa-asg- hsa-asg- 8.70 8.70 8.46 8.62 0.14 100 7.80 8.52 6.487.17 7.59 8.60 6.83 7.84 6.67 7.50 0.77 100 3.5E−02 1.12 2.2 65076507_st2 hsa-asg- hsa-asg- 8.64 8.72 8.31 8.56 0.22 100 7.23 8.44 6.107.33 7.93 7.92 6.86 7.30 7.82 7.44 0.69 89 2.3E−02 1.12 2.2 1019510195_st2 hsa-asg- hsa-asg- 4.98 6.75 6.77 6.17 1.03 67 4.76 5.30 5.144.63 5.24 5.63 4.82 5.11 4.84 5.05 0.32 0 1.1E−02 1.12 2.2 4100 4100_st1hsa-asg- hsa-asg- 6.19 6.60 6.24 6.34 0.22 100 5.07 6.44 4.47 4.76 5.565.75 4.90 5.15 4.95 5.23 0.60 22 1.1E−02 1.12 2.2 8990 8990_st1 hsa-asg-hsa-asg- 8.36 8.77 8.43 8.52 0.22 100 7.55 8.25 5.99 7.25 8.53 8.06 6.707.60 6.70 7.40 0.83 89 4.8E−02 1.12 2.2 6273 6273_st1 hsa-asg- hsa-asg-8.03 8.33 8.21 8.19 0.15 100 7.06 7.66 6.48 6.97 7.51 7.78 6.47 7.096.64 7.07 0.49 100 3.7E−03 1.12 2.2 8626 8626_st1 hsa-miR- hsa-miR- 12.411.8 12.5 12.31 0.37 100 12.1 11.7 10.99 12.2 11.6 11.1 10.8 9.75 10.511.20 0.79 100 4.3E−02 1.11 2.2 152 152_st1 hsa-asg- hsa-asg- 7.09 7.026.84 6.98 0.13 100 6.62 6.79 4.47 5.51 5.63 6.17 5.05 6.56 6.02 5.870.78 44 3.7E−02 1.11 2.2 9574 9574_st2 hsa-asg- hsa-asg- 6.48 7.06 6.616.72 0.31 67 5.35 6.19 5.30 5.40 5.70 5.81 5.35 5.31 6.06 5.61 0.34 05.9E−04 1.11 2.2 9167 9167_st1 hsa-asg- hsa-asg- 6.08 6.38 5.86 6.110.26 100 4.17 5.77 4.75 4.60 5.10 5.68 4.44 5.29 5.18 5.00 0.55 118.0E−03 1.11 2.2 7569 7569_st1 hsa-asg- hsa-asg- 8.00 8.45 8.15 8.200.23 100 7.49 7.92 6.37 6.95 8.00 7.68 6.19 6.88 6.35 7.09 0.70 1002.6E−02 1.11 2.2 10575 10575_st1 hsa-asg- hsa-asg- 9.29 9.37 9.21 9.290.08 100 8.47 9.07 7.13 8.05 8.71 8.76 7.39 8.25 7.79 8.18 0.65 1001.7E−02 1.11 2.2 10392 10392_st1 hsa-asg- hsa-asg- 6.56 6.79 6.70 6.680.11 100 5.83 5.84 4.47 5.42 6.11 6.20 4.69 6.35 5.35 5.58 0.66 561.9E−02 1.10 2.1 4126 4126_st2 hsa- hsa- 9.37 9.52 9.36 9.42 0.09 1008.28 9.05 7.48 8.11 8.51 8.95 7.81 8.32 8.34 8.32 0.50 100 4.0E−03 1.102.1 cand634 cand634_st1 hsa-asg- hsa-asg- 9.54 9.57 9.32 9.47 0.14 1008.22 8.95 7.75 8.20 9.06 8.80 7.64 8.59 8.17 8.38 0.51 100 4.9E−03 1.102.1 10639 10639_st1 hsa-asg- hsa-asg- 9.78 10.0 9.63 9.81 0.20 100 8.539.49 8.14 8.49 8.83 9.72 8.02 9.38 7.83 8.71 0.68 100 2.3E−02 1.10 2.112919 12919_st2 hsa-asg- hsa-asg- 6.04 6.77 6.69 6.50 0.40 67 4.94 6.304.65 5.35 5.54 6.19 4.69 5.46 5.51 5.40 0.59 22 1.3E−02 1.10 2.1 71307130_st1 hsa-asg- hsa-asg- 6.28 6.71 6.68 6.56 0.24 100 5.29 6.65 4.345.39 5.62 6.24 4.64 5.58 5.40 5.46 0.71 22 2.8E−02 1.10 2.1 11691169_st2 hsa-asg- hsa-asg- 7.96 8.06 7.64 7.89 0.22 100 6.27 7.60 5.826.57 7.25 7.49 6.36 6.84 6.90 6.79 0.59 89 1.2E−02 1.10 2.1 39413941_st2 hsa-asg-53 hsa-asg- 9.91 10.1 9.77 9.95 0.21 100 9.57 9.41 8.128.94 9.61 9.62 8.25 8.53 7.65 8.86 0.75 100 3.4E−02 1.10 2.1 53_st2hsa-asg- hsa-asg- 7.84 8.21 7.81 7.95 0.23 0 7.14 7.34 5.74 7.17 7.737.47 6.57 7.01 5.56 6.86 0.76 22 3.8E−02 1.09 2.1 3677 3677_st1 hsa-asg-hsa-asg- 8.68 8.89 8.73 8.77 0.11 100 7.96 8.67 7.07 7.98 8.26 8.25 6.627.17 7.10 7.67 0.70 100 2.6E−02 1.09 2.1 3947 3947_st1 hsa-asg- hsa-asg-10.6 10.7 10.6 10.70 0.07 100 10.6 10.1 9.03 9.64 10.2 10.2 8.97 9.388.17 9.61 0.79 100 4.4E−02 1.09 2.1 6398 6398_st2 hsa-asg- hsa-asg- 6.717.02 6.68 6.81 0.19 100 5.96 6.49 4.98 5.95 5.97 6.41 4.82 5.62 5.275.72 0.59 44 1.2E−02 1.09 2.1 9885 9885_st1 hsa-asg- hsa-asg- 4.21 5.004.83 4.68 0.41 33 3.93 4.86 3.23 3.63 3.85 3.48 1.97 4.14 3.25 3.59 0.790 4.9E−02 1.09 2.1 7327 7327_st1 hsa-asg- hsa-asg- 8.77 9.17 8.90 8.950.20 100 7.88 8.67 7.01 7.28 8.30 8.41 7.21 7.92 8.08 7.86 0.58 1001.1E−02 1.09 2.1 6018 6018_st2 hsa-asg- hsa-asg- 6.49 6.74 6.72 6.650.14 100 5.25 6.46 4.47 5.32 6.44 6.19 4.73 5.72 5.53 5.57 0.71 332.8E−02 1.08 2.1 8446 8446_st2 hsa-asg- hsa-asg- 7.68 7.84 7.87 7.800.10 100 6.55 7.62 6.24 6.23 6.85 7.29 6.02 6.66 6.95 6.71 0.52 1006.2E−03 1.08 2.1 2318 2318_st1 hsa-asg- hsa-asg- 4.88 5.78 5.63 5.430.48 67 4.49 5.08 4.21 3.87 3.68 5.17 4.00 3.74 4.89 4.35 0.58 0 1.6E−021.08 2.1 9560 9560_st2 hsa-asg- hsa-asg- 7.29 7.21 7.55 7.35 0.18 1006.57 6.36 6.43 7.06 6.10 6.13 5.59 5.93 6.25 6.27 0.42 89 1.6E−03 1.082.1 14175 14175_st1 hsa-asg- hsa-asg- 6.62 6.55 6.54 6.57 0.04 100 5.686.18 3.91 5.06 6.25 5.86 5.16 6.03 5.27 5.49 0.74 44 3.3E−02 1.08 2.1331 331_st2 hsa-asg- hsa-asg- 9.09 9.20 9.01 9.10 0.10 100 8.15 8.646.92 8.08 8.88 8.21 7.44 8.11 7.74 8.02 0.59 100 1.2E−02 1.08 2.1 94799479_st2 hsa-asg- hsa-asg- 6.79 7.36 7.25 7.13 0.30 100 6.46 6.98 5.125.85 6.89 6.79 5.12 5.80 5.51 6.06 0.74 44 3.8E−02 1.08 2.1 85468546_st1 hsa-asg- hsa-asg- 6.14 6.38 6.00 6.17 0.19 33 4.37 5.99 4.654.58 5.25 5.75 5.12 5.17 4.97 5.09 0.53 11 7.5E−03 1.08 2.1 72147214_st1 hsa- hsa- 8.26 8.80 8.48 8.51 0.27 100 7.58 8.13 6.44 7.29 8.068.05 6.91 7.46 7.03 7.44 0.58 100 1.2E−02 1.08 2.1 cand559 cand559_st1hsa-asg- hsa-asg- 7.71 7.75 7.77 7.74 0.03 100 6.74 7.36 5.94 6.55 6.827.11 5.66 7.28 6.52 6.67 0.58 78 1.0E−02 1.08 2.1 9883 9883_st1 hsa-asg-hsa-asg- 5.90 5.47 5.82 5.73 0.23 67 4.71 5.33 4.75 4.74 5.21 4.84 3.774.41 4.16 4.66 0.49 0 4.9E−03 1.08 2.1 472 472_st2 hsa-asg- hsa-asg-9.94 10.2 9.91 10.03 0.18 100 9.04 9.46 8.04 8.98 9.81 9.70 8.27 8.938.38 8.96 0.63 67 1.8E−02 1.07 2.1 13079 13079_st2 hsa-asg- hsa-asg-4.54 5.55 6.86 5.65 1.16 33 4.55 5.01 3.73 4.84 4.72 5.01 4.14 4.98 4.204.58 0.46 0 3.5E−02 1.07 2.1 9491 9491_st1 hsa-asg- hsa-asg- 7.34 7.467.29 7.36 0.09 0 6.04 7.10 5.41 5.94 6.93 6.24 5.55 6.77 6.63 6.29 0.6011 1.4E−02 1.07 2.1 3745 3745_st1 hsa-asg- hsa-asg- 9.13 9.72 9.24 9.370.31 100 8.11 8.95 7.19 8.12 9.59 8.66 7.51 8.25 8.26 8.29 0.72 1003.4E−02 1.07 2.1 2228 2228_st1 hsa-asg- hsa-asg- 6.53 6.79 6.30 6.540.25 100 4.76 6.27 5.28 5.14 5.78 6.03 4.33 5.32 6.29 5.47 0.68 442.6E−02 1.07 2.1 4156 4156_st2 hsa-asg- hsa-asg- 5.02 6.07 6.55 5.880.79 33 3.76 5.32 4.17 5.12 4.87 4.57 5.37 4.98 5.12 4.81 0.54 0 2.3E−021.07 2.1 8896 8896_st2 hsa-asg- hsa-asg- 6.47 6.72 6.53 6.57 0.13 675.11 6.01 4.72 5.50 5.50 5.88 4.82 5.94 6.03 5.50 0.51 0 6.0E−03 1.072.1 6250 6250_st2 hsa-asg- hsa-asg- 6.82 7.64 8.13 7.53 0.66 100 6.836.69 5.85 6.49 7.41 7.07 6.38 6.08 5.33 6.46 0.64 78 3.1E−02 1.07 2.112735 12735_st1 hsa-asg- hsa-asg- 5.84 4.95 5.69 5.49 0.48 67 5.45 5.123.54 3.91 5.07 4.55 3.50 4.28 4.40 4.42 0.70 11 3.5E−02 1.07 2.1 30903090_st1 hsa-asg- hsa-asg- 10.7 10.9 10.6 10.79 0.17 100 9.68 10.3 8.789.49 10.3 10.1 9.22 9.72 9.80 9.72 0.52 100 6.3E−03 1.07 2.1 82538253_st2 hsa-asg- hsa-asg- 5.64 6.61 6.54 6.26 0.55 67 5.25 5.81 4.964.88 5.09 6.06 4.82 4.60 5.29 5.19 0.47 22 8.1E−03 1.07 2.1 1194311943_st1 hsa-asg- hsa-asg- 8.55 8.61 8.15 8.44 0.25 0 7.47 7.79 6.356.95 8.73 7.65 7.08 7.19 7.12 7.37 0.66 44 2.4E−02 1.07 2.1 1148611486_st1 hsa-asg- hsa-asg- 7.60 7.92 7.87 7.80 0.17 100 6.78 7.55 5.556.27 7.83 6.09 6.14 7.32 7.02 6.73 0.76 89 4.2E−02 1.07 2.1 89418941_st2 hsa-asg- hsa-asg- 4.81 5.41 4.22 4.81 0.60 33 3.07 4.49 3.233.91 4.50 3.37 3.50 3.64 3.97 3.74 0.52 0 1.3E−02 1.07 2.1 3030 3030_st2hsa-asg- hsa-asg- 10.1 10.1 10.1 10.14 0.04 100 9.38 10.1 8.31 8.73 9.299.42 8.39 9.05 8.96 9.07 0.56 100 9.9E−03 1.07 2.1 5927 5927_st2hsa-asg- hsa-asg- 7.89 8.24 8.28 8.14 0.21 100 6.65 8.30 5.94 6.70 7.327.98 6.54 6.99 7.22 7.07 0.73 89 3.6E−02 1.07 2.1 976 976_st2 hsa-asg-hsa-asg- 6.43 6.89 7.14 6.82 0.36 67 5.51 6.28 5.26 5.75 6.45 6.14 5.405.89 5.12 5.76 0.47 11 5.3E−03 1.07 2.1 1319 1319_st2 hsa-asg- hsa-asg-8.08 7.98 7.99 8.02 0.05 0 6.25 7.26 6.71 7.29 6.63 7.22 6.29 7.50 7.416.95 0.48 33 4.2E−03 1.06 2.1 7131 7131_st1 hsa- hsa- 5.52 6.54 5.825.96 0.52 33 4.99 5.85 4.26 4.69 5.32 5.09 4.78 4.66 4.43 4.90 0.48 118.8E−03 1.06 2.1 cand960 cand960_st2 hsa-asg- hsa-asg- 5.44 5.78 5.935.72 0.25 100 4.49 5.64 4.30 4.04 4.21 5.09 4.49 4.52 5.09 4.65 0.52 117.1E−03 1.06 2.1 8508 8508_st2 hsa-asg- hsa-asg- 10.2 10.3 10.0 10.200.17 100 9.40 9.72 8.49 8.88 9.39 9.50 8.32 9.40 9.16 9.14 0.48 784.2E−03 1.06 2.1 12681 12681_st1 hsa-asg- hsa-asg- 9.41 9.69 9.52 9.540.14 100 8.57 9.15 7.29 8.36 9.18 9.10 7.88 8.50 8.27 8.48 0.63 111.8E−02 1.06 2.1 7400 7400_st1 hsa-asg- hsa-asg- 5.58 6.12 6.18 5.960.33 100 4.61 5.46 4.75 4.88 5.21 4.97 4.27 5.32 4.60 4.90 0.39 01.7E−03 1.06 2.1 4246 4246_st2 hsa-asg- hsa-asg- 7.03 7.63 7.72 7.460.37 100 6.14 7.02 5.97 6.35 6.51 6.89 5.75 6.57 6.40 6.40 0.41 672.7E−03 1.06 2.1 9209 9209_st2 hsa-asg- hsa-asg- 4.92 5.39 5.06 5.120.24 67 4.02 4.86 2.86 4.15 4.60 4.75 3.17 3.74 4.40 4.06 0.69 0 3.0E−021.06 2.1 9504 9504_st1 hsa-asg- hsa-asg- 7.83 8.29 8.39 8.17 0.30 0 6.777.73 5.80 6.88 7.26 7.67 6.47 7.32 8.11 7.11 0.71 22 3.4E−02 1.06 2.11241 1241_st2 hsa-asg- hsa-asg- 6.50 6.73 6.66 6.63 0.12 100 5.68 5.256.12 6.30 5.59 5.60 5.35 5.06 5.20 5.57 0.42 11 1.8E−03 1.06 2.1 1118111181_st1 hsa-asg- hsa-asg- 8.42 8.62 8.16 8.40 0.23 100 7.13 8.08 6.607.54 8.14 7.94 6.18 7.11 7.35 7.34 0.67 100 2.6E−02 1.06 2.1 41464146_st2 hsa-asg- hsa-asg- 6.57 6.80 6.77 6.71 0.12 100 5.78 6.40 4.655.51 5.97 6.19 4.94 6.02 5.44 5.65 0.58 22 1.2E−02 1.06 2.1 1165211652_st1 hsa-asg- hsa-asg- 10.1 10.3 10.3 10.26 0.12 100 10.4 9.69 8.359.15 9.77 9.22 8.72 9.63 7.86 9.20 0.79 100 4.9E−02 1.05 2.1 1360613606_st2 hsa-asg- hsa-asg- 7.11 6.97 6.88 6.99 0.12 100 5.92 6.92 4.725.75 6.24 6.30 5.22 6.12 6.24 5.94 0.65 22 2.2E−02 1.05 2.1 29912991_st1 hsa-asg- hsa-asg- 9.11 9.83 8.91 9.28 0.48 100 8.25 8.62 7.038.27 9.33 8.51 7.91 8.07 8.13 8.23 0.61 100 2.3E−02 1.05 2.1 86078607_st1 hsa-asg- hsa-asg- 7.14 7.17 7.08 7.13 0.04 100 6.27 6.87 4.726.39 6.22 6.86 5.69 5.98 5.71 6.08 0.67 67 2.4E−02 1.05 2.1 35253525_st1 hsa-asg- hsa-asg- 9.34 9.45 9.25 9.35 0.10 67 8.79 8.83 7.348.15 8.89 8.84 7.86 8.13 7.85 8.30 0.56 11 1.1E−02 1.05 2.1 11331133_st2 hsa-asg- hsa-asg- 4.21 4.47 4.59 4.43 0.20 33 3.21 4.30 1.923.28 3.72 4.03 2.78 3.64 3.53 3.38 0.71 0 3.3E−02 1.05 2.1 4335 4335_st1hsa-asg- hsa-asg- 7.36 7.07 7.43 7.29 0.19 100 6.32 7.09 6.03 6.08 6.146.40 5.69 6.03 6.41 6.24 0.39 78 1.3E−03 1.05 2.1 11938 11938_st1hsa-asg-92 hsa-asg- 5.62 5.51 5.52 5.55 0.06 100 4.17 5.58 4.02 4.154.27 5.20 3.85 4.21 5.14 4.51 0.62 11 1.8E−02 1.04 2.1 92_st1 hsa-asg-hsa-asg- 5.99 6.02 6.45 6.15 0.26 100 4.61 5.79 4.54 4.71 5.10 5.82 4.075.69 5.63 5.11 0.65 22 2.5E−02 1.04 2.1 12306 12306_st2 hsa-asg-hsa-asg- 10.6 10.6 10.4 10.56 0.13 100 9.82 10.0 8.85 9.26 10.2 9.758.98 9.42 9.27 9.52 0.49 100 5.4E−03 1.04 2.1 11389 11389_st1 hsa- hsa-5.08 5.75 6.13 5.65 0.53 100 3.93 5.15 3.91 4.49 5.76 5.27 4.86 3.834.32 4.61 0.69 33 3.9E−02 1.04 2.1 cand443 cand443_st2 hsa-miR- hsa-miR-10.5 10.1 10.5 10.41 0.25 100 10.2 9.76 9.14 9.80 9.38 9.47 9.04 8.519.05 9.37 0.50 100 7.2E−03 1.04 2.1 151 151_st2 hsa-asg- hsa-asg- 6.506.96 6.51 6.65 0.26 100 5.65 6.90 5.39 5.25 5.80 6.29 5.43 5.02 4.815.62 0.65 22 2.5E−02 1.04 2.1 10807 10807_st2 hsa-asg- hsa-asg- 7.958.56 8.19 8.24 0.31 100 7.20 7.68 6.42 6.56 7.83 7.72 6.47 7.46 7.437.20 0.57 100 1.4E−02 1.04 2.1 9627 9627_st1 hsa-asg- hsa-asg- 6.37 6.756.68 6.60 0.21 100 5.18 6.41 5.45 5.28 5.58 6.46 4.90 4.96 5.87 5.570.58 22 1.4E−02 1.04 2.1 3553 3553_st1 hsa-asg- hsa-asg- 7.95 7.92 7.687.85 0.15 100 6.39 7.83 5.82 6.51 7.22 7.13 6.32 7.06 7.06 6.82 0.61 891.7E−02 1.04 2.0 8687 8687_st1 hsa-asg- hsa-asg- 10.7 10.9 10.6 10.760.17 100 10.1 10.2 9.52 9.55 10.3 10.0 9.14 9.69 8.95 9.73 0.48 1005.3E−03 1.03 2.0 8568 8568_st1 hsa-asg- hsa-asg- 9.74 10.2 9.89 9.950.24 100 9.24 9.50 7.87 8.25 9.62 9.02 8.28 9.25 9.18 8.91 0.62 1002.0E−02 1.03 2.0 8520 8520_st1 hsa-asg- hsa-asg- 7.88 7.67 7.51 7.690.19 0 6.57 7.08 6.28 6.48 7.01 7.04 5.58 7.09 6.77 6.65 0.50 11 6.7E−031.03 2.0 12092 12092_st1 hsa-asg- hsa-asg- 5.50 6.40 6.33 6.08 0.50 675.92 5.21 4.58 4.97 5.83 4.31 4.86 4.44 5.29 5.04 0.57 0 1.9E−02 1.032.0 5888 5888_st1 hsa-asg- hsa-asg- 5.37 5.26 4.86 5.16 0.27 100 4.315.27 4.47 4.08 4.11 4.55 3.40 4.28 2.74 4.13 0.72 11 3.9E−02 1.03 2.09903 9903_st2 hsa-asg- hsa-asg- 9.89 10.1 9.79 9.93 0.17 100 9.19 9.278.30 9.24 9.56 9.37 8.56 8.50 8.11 8.90 0.53 100 9.2E−03 1.03 2.0 19031903_st2 hsa-asg- hsa-asg- 10.7 10.9 10.7 10.82 0.08 100 10.1 10.3 8.769.92 10.8 10.2 9.27 9.68 8.93 9.79 0.69 100 3.2E−02 1.03 2.0 89588958_st1 hsa-asg- hsa-asg- 5.42 5.53 5.67 5.54 0.13 100 4.37 5.46 3.394.36 4.21 5.13 4.14 4.47 5.09 4.51 0.63 11 2.1E−02 1.03 2.0 70677067_st1 hsa-asg- hsa-asg- 8.83 8.94 8.56 8.77 0.19 0 8.25 8.32 7.037.40 7.91 7.99 6.95 7.89 8.00 7.75 0.50 78 7.1E−03 1.03 2.0 1133611336_st2 hsa-asg- hsa-asg- 7.20 7.48 7.41 7.36 0.14 100 6.37 7.44 5.726.10 6.64 7.07 4.94 6.37 6.40 6.34 0.73 78 4.0E−02 1.03 2.0 85628562_st1 hsa-asg- hsa-asg- 8.53 8.96 8.78 8.76 0.21 100 7.09 8.64 6.667.37 8.40 8.44 6.93 7.75 8.30 7.73 0.74 100 4.5E−02 1.02 2.0 1151311513_st1 hsa-asg- hsa-asg- 5.26 6.01 5.63 5.63 0.37 67 4.85 4.76 4.545.12 4.55 4.18 4.98 4.35 4.16 4.61 0.34 0 1.3E−03 1.02 2.0 128 128_st2hsa-asg- hsa-asg- 4.52 5.11 4.83 4.82 0.30 33 3.45 4.43 3.06 4.15 2.904.43 3.50 3.99 4.24 3.79 0.58 0 1.7E−02 1.02 2.0 13754 13754_st2hsa-asg- hsa-asg- 10.6 11.0 10.6 10.81 0.24 100 9.50 10.0 8.74 9.61 10.710.2 9.42 9.99 9.69 9.79 0.58 89 1.6E−02 1.02 2.0 11623 11623_st1hsa-miR-518c* hsa-miR- 5.23 5.03 4.96 5.07 0.14 33 3.85 5.19 4.39 3.153.39 4.43 3.68 3.74 4.67 4.05 0.66 0 2.7E−02 1.02 2.0 518c-_st2 hsa-asg-hsa-asg- 8.06 8.11 8.00 8.06 0.06 100 7.38 8.09 6.15 6.88 7.28 7.11 6.337.10 7.00 7.04 0.57 89 1.3E−02 1.02 2.0 8188 8188_st1 hsa-asg- hsa-asg-6.61 6.74 6.53 6.62 0.11 100 5.60 6.24 5.41 5.30 5.68 5.89 5.09 5.675.56 5.60 0.33 44 4.8E−04 1.02 2.0 4836 4836_st2 hsa-asg- hsa-asg- 6.926.51 6.85 6.76 0.22 100 5.78 6.21 5.58 5.63 6.15 6.22 4.86 5.68 5.555.74 0.43 33 3.1E−03 1.02 2.0 4564 4564_st2 hsa-asg- hsa-asg- 5.62 5.975.71 5.77 0.18 100 4.55 5.55 4.75 4.00 4.70 4.93 3.93 4.96 5.39 4.750.55 11 1.1E−02 1.02 2.0 11898 11898_st2 hsa-asg- hsa-asg- 7.66 8.077.64 7.79 0.25 100 7.11 7.20 6.15 7.09 7.86 7.14 6.02 6.63 5.74 6.770.69 78 3.4E−02 1.02 2.0 7227 7227_st1 hsa-asg- hsa-asg- 9.69 9.87 9.759.77 0.09 100 8.30 9.69 7.88 8.33 8.72 9.50 8.07 9.09 9.19 8.75 0.65 442.5E−02 1.02 2.0 4851 4851_st1 hsa-asg- hsa-asg- 9.54 9.72 9.64 9.640.09 100 9.49 9.56 7.69 8.32 9.00 9.12 7.74 8.71 7.94 8.62 0.73 1004.1E−02 1.02 2.0 8631 8631_st1 hsa-asg- hsa-asg- 7.25 7.64 7.37 7.420.20 100 6.14 7.24 5.55 6.20 6.69 7.15 5.75 6.61 6.32 6.41 0.58 561.5E−02 1.02 2.0 9501 9501_st1 hsa-asg- hsa-asg- 6.97 7.87 7.91 7.580.53 100 6.54 7.38 6.10 7.10 6.15 6.97 6.36 5.98 6.56 6.57 0.48 781.1E−02 1.01 2.0 6924 6924_st1 hsa-asg- hsa-asg- 6.99 7.27 7.21 7.160.15 100 5.35 7.02 5.55 5.92 6.26 6.79 5.29 6.31 6.82 6.14 0.66 442.8E−02 1.01 2.0 7069 7069_st1 hsa-asg- hsa-asg- 5.74 6.26 5.94 5.980.26 33 5.70 5.28 4.43 4.04 5.25 5.49 4.38 5.17 4.97 4.97 0.56 0 1.4E−021.01 2.0 11791 11791_st2 hsa-asg- hsa-asg- 6.99 7.46 7.05 7.17 0.26 1005.39 6.57 5.69 5.86 5.99 6.74 6.29 6.10 6.78 6.16 0.48 67 6.7E−03 1.012.0 13893 13893_st2 hsa-asg- hsa-asg- 7.49 7.84 7.61 7.65 0.18 100 6.187.52 5.88 6.35 6.78 7.52 6.11 6.36 7.02 6.64 0.61 89 2.0E−02 1.01 2.07883 7883_st2 hsa-asg- hsa-asg- 9.31 9.72 9.38 9.47 0.22 67 8.61 8.847.44 8.12 9.18 8.91 7.87 8.78 8.36 8.46 0.56 11 1.3E−02 1.01 2.0 70977097_st2 hsa-asg- hsa-asg- 11.5 11.5 11.3 11.47 0.14 100 10.7 11.1 9.8710.2 11.0 10.9 9.76 10.4 10.0 10.46 0.52 100 9.1E−03 1.01 2.0 1168511685_st2 hsa-asg- hsa-asg- 8.04 8.13 8.09 8.09 0.04 100 6.51 7.96 6.736.92 6.98 7.83 6.55 6.76 7.50 7.08 0.55 100 1.1E−02 1.00 2.0 1384113841_st2 hsa-asg- hsa-asg- 8.10 8.24 8.18 8.17 0.07 100 7.00 8.19 6.226.63 7.72 7.81 6.78 6.86 7.32 7.17 0.64 100 2.4E−02 1.00 2.0 37103710_st1 hsa-asg- hsa-asg- 5.44 5.57 5.23 5.41 0.17 33 4.49 4.55 4.683.95 4.21 4.24 4.27 4.21 5.09 4.41 0.34 0 6.6E−04 1.00 2.0 1379113791_st2 hsa- hsa- 8.97 9.39 8.90 9.09 0.27 100 8.13 8.64 6.93 7.818.90 8.44 7.47 8.06 8.36 8.08 0.61 100 2.2E−02 1.00 2.0 cand924cand924_st2 hsa-asg- hsa-asg- 5.14 5.39 4.11 4.88 0.68 33 4.02 4.37 3.673.73 4.21 4.65 3.17 2.78 4.32 3.88 0.61 0 3.6E−02 1.00 2.0 8022 8022_st2hsa-asg- hsa-asg- 6.83 7.13 7.19 7.05 0.19 100 6.06 6.85 5.33 5.97 6.446.72 5.02 6.32 5.75 6.05 0.61 67 2.2E−02 1.00 2.0 8628 8628_st2 hsa-asg-hsa-asg- 7.01 7.11 7.02 7.05 0.06 100 6.25 6.49 5.85 5.82 6.11 5.99 5.326.29 6.30 6.05 0.35 11 7.9E−04 1.00 2.0 8490 8490_st1 hsa-asg- hsa-asg-10.0 10.3 10.2 10.21 0.14 100 9.19 9.82 8.18 8.98 9.39 9.86 8.97 9.009.46 9.21 0.51 78 8.9E−03 1.00 2.0 1643 1643_st2 SD, standard deviation;%, percentage of samples expressing miRNA above background levels;Log2Diff, difference in VSN-transformed expression between NBR and LNnegsamples.

Example 20 miRNAs Differentally Expressed Between Normal Bladder Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous bladder cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal bladdertissue than in cancer-negative lymph nodes.

Total RNA, purified from three separate normal human bladder tissuesamples (FirstChoice® Human Bladder Total RNA, cat. no. AM7990; Ambion,Austin, Tex., USA) and from nine normal human lymph node samples(FirstChoice® Human Lymph Node Total RNA, cat. no. AM7894; Ambion,Austin, Tex., USA) was purchased from Ambion. miRNA purification andlabeling and microarray expression analyses were performed as describedabove in Example 2.

The inventors identified 303 miRNAs (142 verified, 161 predicted) withsignificant differential expression between normal bladder tissuesamples and normal lymph nodes (log 2Diff bladder vs LN neg≧1 andp-value<0.05) (Table 19). Among the verified miRNAs, hsa-miR-205 andhsa-miR-187 had average expression levels that were more than 100-foldhigher in bladder tissues; hsa-miR-429 and hsa-miR-133b had averageexpression levels that were more than 50-fold higher in bladder tissues;21 miRNAs had average expression levels that were between 10- and40-fold higher in bladder tissue samples; and 41 miRNAs had averageexpression levels that were between 5- and 10-fold higher in bladdertissue samples. Among the predicted miRNAs, hsa-asg-6845_st2 had anaverage expression level that was over 50-fold higher in bladder tissuesamples; 11 miRNAs had average expression levels that were between 10-and 20-fold higher in bladder samples; and 43 miRNAs had averageexpression levels that were between 5- and 10-fold higher in bladdertissue samples.

TABLE 19 MicroRNAs Differentially Expressed Between Normal BladderSamples (NBL) and Cancer-Negative Lymph Node Samples (LNneg) fromPatients. Probe NBL LNneg MicroRNA ID 1 2 3 M SD % 1 2 3 4 5 6 7 8 9 MSD % ttest log2 Diff Fold hsa-miR- hsa-miR- 12.9 12.5 13.30 12.9 0.36100 2.93 5.91 5.64 2.03 3.59 6.11 3.40 5.32 7.76 4.74 1.85 33  2.3E−058.18 290 205 205_st1 hsa-miR- hsa-miR- 10.5 10.5 9.71 10.2 0.47 100 4.762.79 2.76 3.77 3.22 3.37 3.05 3.38 3.17 3.37 0.61 0  7.0E−09 6.89 118187 187_st1 hsa-miR- hsa-miR- 8.66 7.84 9.75 8.75 0.96 100 3.45 1.543.67 2.13 2.37 2.69 2.32 2.45 5.39 2.89 1.14 0  1.2E−05 5.86 58.1 429429_st1 hsa-miR- hsa-miR- 12.2 12.1 12.54 12.3 0.19 100 7.36 6.22 4.4710.1 7.45 6.20 7.22 4.47 5.39 6.55 1.78 67  2.9E−04 5.78 55.0 133b133b_st2 hsa-asg- hsa-asg- 8.26 8.55 8.45 8.42 0.15 100 2.61 3.10 2.433.15 2.69 2.85 2.23 2.45 3.09 2.73 0.33 0  8.0E−11 5.69 51.5 68456845_st2 hsa-miR- hsa-miR- 12.8 12.7 13.05 12.8 0.14 100 7.77 7.70 5.9210.8 8.19 7.14 8.22 6.25 6.48 7.61 1.47 89  1.2E−04 5.28 38.9 133a133a_st1 hsa-miR- hsa-miR- 11.9 11.1 12.46 11.8 0.68 100 7.33 5.89 7.218.24 6.45 5.41 5.19 6.07 9.36 6.79 1.37 78  1.4E−04 5.02 32.5 203203_st2 hsa-miR- hsa-miR- 10.1 9.77 10.29 10.0 0.27 100 6.08 5.79 4.937.23 6.18 4.55 4.94 3.54 4.95 5.36 1.08 44  2.8E−05 4.70 26.1 189189_st1 hsa-miR- hsa-miR- 8.72 9.00 9.81 9.17 0.57 100 5.85 2.16 4.545.96 4.89 4.10 4.07 4.35 4.67 4.51 1.12 22  4.7E−05 4.66 25.4 582582_st1 hsa-miR- hsa-miR- 12.4 11.8 12.65 12.2 0.43 100 7.62 7.80 8.906.69 6.82 6.87 6.51 7.65 10.4 7.70 1.26 100  1.3E−04 4.59 24.2 200b200b_st1 hsa-miR- hsa-miR- 9.93 9.34 11.03 10.1 0.86 100 6.27 5.08 6.495.72 5.29 4.60 4.07 4.78 7.78 5.56 1.14 67  9.4E−05 4.54 23.2 200a200a_st2 hsa-miR- hsa-miR- 13.6 13.0 13.74 13.4 0.38 100 9.60 8.84 10.38.18 8.15 8.14 7.98 8.89 11.5 9.07 1.21 100  1.2E−04 4.42 21.3 200c200c_st1 hsa-asg- hsa-asg- 7.01 7.70 8.50 7.73 0.75 100 4.66 4.30 3.394.46 2.69 2.77 2.15 2.78 3.53 3.41 0.89 0  2.1E−05 4.32 20.0 924 924_st1hsa-miR- hsa-miR- 7.95 8.29 7.87 8.04 0.22 100 4.85 3.74 4.54 5.23 5.562.99 3.40 2.27 2.10 3.85 1.27 11  2.5E−04 4.19 18.2 411 411_st1 hsa-miR-hsa-miR- 8.14 8.04 8.66 8.28 0.34 100 4.71 4.46 4.84 6.39 5.46 2.17 6.163.59 0.63 4.27 1.88 22  5.0E−03 4.01 16.2 378 378_st1 hsa-asg- hsa-asg-7.34 5.46 7.42 6.74 1.11 67 3.07 2.26 3.06 3.01 2.37 2.69 1.16 2.78 4.402.75 0.86 0  6.5E−05 3.99 15.9 14153 14153_st2 hsa-asg- hsa-asg- 7.498.04 7.66 7.73 0.28 100 3.56 3.47 3.79 6.15 4.68 3.37 3.73 1.48 3.773.78 1.22 0  3.1E−04 3.95 15.5 5602 5602_st2 hsa-miR- hsa-miR- 8.16 8.818.74 8.57 0.35 100 4.37 3.83 6.88 6.18 6.33 3.69 4.38 1.88 4.60 4.681.56 33  2.0E−03 3.89 14.8 493-5p 493-5p_st1 hsa-miR- hsa-miR- 10.0 10.310.01 10.1 0.21 100 6.49 6.52 7.70 8.08 7.70 5.24 5.64 3.99 5.09 6.271.39 67  9.2E−04 3.86 14.6 368 368_st2 hsa-miR- hsa-miR- 6.44 6.91 7.076.80 0.33 100 4.31 2.54 2.86 3.15 1.83 1.78 3.05 2.62 4.54 2.96 0.96 0 5.8E−05 3.84 14.3 592 592_st1 hsa-miR- hsa-asg- 6.40 6.90 6.81 6.700.26 100 3.07 3.29 3.67 1.72 1.64 2.69 3.05 1.68 5.42 2.92 1.21 11 3.8E−04 3.79 13.8 592 4400_st1 hsa-miR- hsa-miR- 6.62 5.89 6.57 6.360.41 100 2.61 2.16 3.06 0.75 1.74 2.17 2.92 3.07 4.47 2.55 1.03 0 1.2E−04 3.81 14.0 605 605_st1 hsa-miR- hsa-miR- 9.11 9.17 8.91 9.060.13 100 6.02 4.81 6.97 6.26 6.32 4.43 5.16 2.45 4.89 5.26 1.35 56 8.0E−04 3.81 14.0 495 495_st1 hsa-asg- hsa-asg- 7.55 7.43 8.26 7.740.45 100 3.56 3.53 1.66 5.23 4.47 4.24 4.27 4.21 4.54 3.97 1.01 0 1.0E−04 3.78 13.7 2027 2027_st1 hsa-miR- hsa-miR- 8.73 8.42 8.28 8.480.23 100 5.73 4.49 5.80 6.38 5.99 2.99 5.05 2.45 3.97 4.76 1.39 44 1.1E−03 3.71 13.1 299-5p 299-5p_st2 hsa-asg- hsa-asg- 6.49 6.98 6.956.81 0.27 100 4.71 2.06 3.31 5.25 3.10 1.97 2.48 3.38 1.74 3.11 1.22 0 5.0E−04 3.70 13.0 10202 10202_st2 hsa-asg- hsa-asg- 8.57 8.37 8.08 8.340.24 100 5.18 4.43 6.99 6.06 5.91 3.54 4.59 3.07 2.10 4.65 1.57 22 2.8E−03 3.69 12.9 5021 5021_st1 hsa-miR- hsa-miR- 9.53 9.11 9.20 9.280.22 100 6.06 5.84 6.97 6.90 6.25 5.40 4.90 3.74 4.40 5.61 1.10 67 2.3E−04 3.67 12.8 487b 487b_st2 hsa-asg- hsa-asg- 6.62 6.55 6.49 6.560.07 100 3.56 2.87 2.86 4.33 3.39 2.69 1.79 1.48 3.65 2.96 0.90 0 5.5E−05 3.60 12.1 3910 3910_st2 hsa-miR-31 hsa-miR- 12.5 12.6 13.1512.8 0.31 100 10.4 9.79 8.95 10.1 8.71 8.60 7.73 8.68 10.1 9.23 0.91 100 7.2E−05 3.56 11.8 31_st2 hsa-miR- hsa-miR- 6.76 7.22 7.16 7.04 0.25 1003.76 2.54 4.30 4.36 4.91 3.13 3.17 1.29 3.97 3.49 1.10 0  3.1E−04 3.5511.7 154 154_st2 hsa-asg- hsa-asg- 5.45 6.06 6.26 5.92 0.42 100 1.652.54 2.43 3.63 1.46 2.53 3.17 2.62 1.38 2.38 0.76 0  2.0E−05 3.54 11.72345 2345_st2 hsa-asg- hsa-asg- 8.58 8.44 8.27 8.43 0.15 100 5.60 5.376.65 5.89 5.90 4.03 4.73 2.45 3.39 4.89 1.36 56  1.4E−03 3.54 11.6 1325413254_st1 hsa-miR- hsa-miR- 8.94 7.03 10.67 8.88 1.82 100 4.09 4.99 1.929.39 3.97 5.85 8.46 4.47 5.00 5.35 2.31 44  3.8E−02 3.53 11.6 206206_st2 hsa-miR- hsa-miR- 6.27 6.86 6.78 6.64 0.32 100 2.78 2.36 4.213.40 4.21 2.99 3.50 1.29 3.25 3.11 0.91 0  8.0E−05 3.53 11.5 381 381_st2hsa-asg- hsa-asg- 6.99 7.43 7.77 7.40 0.39 100 4.90 2.06 3.60 5.83 4.852.85 4.73 3.78 2.44 3.89 1.28 0  1.0E−03 3.50 11.3 13284 13284_st1hsa-miR-376a hsa-miR- 7.80 8.05 7.48 7.78 0.28 100 5.14 4.08 5.49 5.674.85 4.03 2.48 3.83 3.77 4.37 1.01 0  2.3E−04 3.41 10.6 376a_st2hsa-miR- hsa-asg- 6.83 7.26 6.77 6.95 0.27 100 3.33 4.30 3.39 4.00 4.853.69 2.92 4.07 1.86 3.60 0.87 0  8.0E−05 3.35 10.2 376a 14234_st1hsa-miR- hsa-asg- 7.88 8.47 8.05 8.13 0.30 100 5.03 4.91 6.09 5.56 5.354.10 3.85 4.14 4.67 4.85 0.74 11  2.6E−05 3.28 9.7 376a 10049_st1hsa-asg- hsa-asg- 6.42 5.62 6.39 6.14 0.45 100 3.76 3.29 3.91 3.63 3.442.69 1.42 1.48 1.62 2.80 1.03 0  3.4E−04 3.34 10.1 5617 5617_st1hsa-miR- hsa-miR- 10.1 10.2 10.41 10.2 0.15 100 9.10 7.83 4.98 9.74 8.827.06 6.80 3.64 4.89 6.99 2.11 67  2.7E−02 3.26 9.6 196b 196b_st2hsa-asg- hsa-asg- 4.46 4.62 6.69 5.25 1.25 33 2.25 3.02 1.66 2.03 2.192.44 0.48 1.48 2.54 2.01 0.74 0  2.1E−04 3.24 9.5 791 791_st1 hsa-miR-hsa-miR- 7.78 7.21 7.71 7.57 0.31 100 5.11 4.08 5.57 4.86 4.04 2.17 4.783.44 4.89 4.33 1.03 11  4.0E−04 3.24 9.4 338 338_st1 hsa-miR- hsa-miR-8.52 8.91 9.41 8.95 0.45 100 5.80 6.16 5.53 5.92 5.78 3.48 5.82 5.727.23 5.72 0.97 89  2.8E−04 3.23 9.4 424 424_st1 hsa-miR-14205_st1hsa-asg- 9.59 9.44 10.01 9.68 0.29 100 7.53 5.85 7.37 7.77 6.85 4.776.64 5.46 5.93 6.47 1.02 56  3.9E−04 3.22 9.3 331 hsa-miR- hsa-miR- 10.710.7 11.16 10.8 0.25 100 9.04 7.57 8.35 8.79 7.69 7.09 7.70 7.28 7.207.86 0.71 100  3.5E−05 3.02 8.1 331 331_st2 hsa-asg- hsa-asg- 9.84 9.419.54 9.60 0.22 100 7.02 6.39 7.09 7.51 6.90 6.04 6.76 5.59 4.40 6.410.95 67  2.3E−04 3.19 9.1 13205 13205_st1 hsa-miR- hsa-miR- 9.59 9.939.62 9.72 0.19 100 6.81 5.57 7.95 7.53 7.69 5.72 6.59 5.49 5.44 6.531.02 56  3.9E−04 3.18 9.1 127 127_st1 hsa-asg- hsa-asg- 7.60 5.85 7.466.97 0.97 67 3.66 2.54 4.47 3.46 3.93 4.03 3.29 3.64 5.27 3.81 0.76 0 1.5E−04 3.16 9.0 7637 7637_st2 hsa-asg- hsa-asg- 5.97 5.59 5.96 5.840.22 100 2.78 1.54 2.43 3.91 2.83 2.85 1.79 3.54 2.54 2.69 0.75 0 3.8E−05 3.15 8.9 10393 10393_st1 hsa-asg- hsa-asg- 6.43 5.97 5.33 5.910.55 67 1.25 2.63 5.30 4.63 1.83 2.53 2.92 1.39 2.44 2.77 1.38 0 3.8E−03 3.14 8.8 14065 14065_st2 hsa-asg- hsa-asg- 6.38 6.17 6.52 6.360.18 100 3.33 3.16 3.54 2.51 2.53 3.26 2.64 3.20 5.09 3.25 0.78 0 6.1E−05 3.10 8.6 10131 10131_st2 hsa-asg-14172 hsa-asg- 7.08 7.19 7.507.26 0.22 100 5.07 2.71 4.47 5.45 4.81 2.77 3.59 3.74 5.18 4.20 1.03 0 5.9E−04 3.06 8.3 14172_st1 hsa-miR- hsa-miR- 8.41 7.77 7.36 7.85 0.53100 4.55 5.43 5.92 5.53 5.77 5.68 3.50 4.28 2.74 4.82 1.13 22  1.3E−033.03 8.1 432 432_st2 hsa-asg- hsa-asg- 5.14 4.21 5.45 4.93 0.64 33 1.852.36 2.18 1.51 2.11 1.78 0.62 1.48 3.39 1.92 0.75 0  1.0E−04 3.01 8.111715 11715_st2 hsa-miR- hsa-miR- 11.6 11.8 11.87 11.8 0.10 100 9.629.27 8.82 9.68 9.16 8.64 7.76 7.45 8.75 8.80 0.77 100  6.4E−05 3.01 8.0218 218_st2 hsa-asg- hsa-asg- 7.71 7.44 7.31 7.49 0.20 100 5.62 5.682.96 5.39 5.57 4.03 3.59 2.70 4.81 4.48 1.19 11  1.7E−03 3.00 8.0 1039110391_st2 hsa-asg- hsa-asg- 6.01 4.89 5.05 5.32 0.61 67 2.61 2.71 2.183.52 1.83 1.78 2.78 1.11 2.33 2.32 0.70 0  6.0E−05 3.00 8.0 1214212142_st1 hsa-asg- hsa-asg- 5.90 5.08 5.61 5.53 0.42 67 2.78 2.16 4.021.72 2.19 2.35 0.35 3.20 3.97 2.53 1.14 0  1.4E−03 3.00 8.0 1024710247_st1 hsa-miR- hsa-miR- 9.58 8.59 9.43 9.20 0.54 100 7.18 5.82 5.988.21 6.76 5.46 5.19 5.42 5.78 6.20 0.99 44  6.1E−04 3.00 8.0 204 204_st2hsa-asg- hsa-asg- 8.73 8.61 8.63 8.66 0.07 100 5.60 5.72 5.67 6.74 5.705.30 5.25 5.00 6.09 5.68 0.51 56  2.0E−06 2.98 7.9 7471 7471_st1hsa-asg- hsa-asg- 5.45 5.39 5.05 5.30 0.21 67 2.43 3.42 2.43 3.82 2.191.19 0.76 2.27 2.33 2.32 0.95 0  3.7E−04 2.98 7.9 6178 6178_st2 hsa-miR-hsa-asg- 11.2 10.8 11.31 11.1 0.24 100 8.22 8.12 8.48 8.62 7.79 8.037.78 8.78 8.07 8.21 0.35 100  1.0E−07 2.95 7.8 421 5033_st2 hsa-miR-hsa-miR- 12.0 11.5 12.04 11.8 0.26 100 9.50 9.20 8.98 9.42 8.71 8.848.37 9.39 8.91 9.04 0.37 100  2.8E−07 2.84 7.2 421 421-3p_st1 hsa-asg-hsa-asg- 5.22 4.62 5.91 5.25 0.65 33 1.25 1.05 3.85 1.82 0.94 2.35 1.603.38 4.50 2.31 1.31 0  4.3E−03 2.94 7.7 11088 11088_st1 hsa-miR-hsa-miR- 7.86 8.56 8.89 8.43 0.52 100 7.18 6.53 4.21 5.98 6.52 4.70 4.864.35 5.09 5.49 1.08 44  1.2E−03 2.94 7.7 101 101_st1 hsa-miR- hsa-asg-6.18 5.82 6.36 6.12 0.28 100 1.65 3.16 3.79 3.73 4.42 2.35 3.68 2.783.09 3.18 0.84 0  1.8E−04 2.94 7.7 329 13589_st2 hsa-miR- hsa-miR- 6.596.30 7.26 6.72 0.49 100 4.24 2.71 2.86 3.91 3.63 4.03 4.20 4.47 4.073.79 0.61 0  2.2E−05 2.93 7.6 450 450_st2 hsa-miR- hsa-asg- 5.82 4.985.78 5.53 0.47 67 3.56 2.16 2.43 3.01 2.53 3.13 2.92 2.93 2.74 2.82 0.410  2.5E−06 2.71 6.5 450 14228_st2 hsa-asg- hsa-asg- 9.57 9.07 9.41 9.350.26 100 7.05 6.35 7.01 7.73 6.67 5.80 5.79 5.15 6.39 6.44 0.78 11 1.0E−04 2.91 7.5 14150 14150_st1 hsa-asg- hsa-asg- 5.31 4.68 5.54 5.180.45 67 1.65 0.95 3.79 2.33 2.69 2.35 0.35 3.20 3.25 2.29 1.12 0 1.7E−03 2.89 7.4 13705 13705_st1 hsa-asg- hsa-asg- 6.36 5.46 5.85 5.890.45 33 2.61 2.45 3.14 4.86 4.45 1.29 2.48 2.17 3.59 3.00 1.13 0 1.8E−03 2.88 7.4 7469 7469_st1 hsa-asg- hsa-asg- 4.99 3.76 5.32 4.690.82 33 2.25 1.96 2.66 2.03 2.53 1.01 0.02 2.17 1.62 1.80 0.83 0 3.9E−04 2.88 7.4 8428 8428_st2 hsa-miR- hsa-miR- 6.92 6.06 8.24 7.071.10 100 5.07 3.83 4.93 4.43 4.39 2.85 3.85 3.83 4.54 4.19 0.68 0 2.5E−04 2.88 7.4 141 141_st1 hsa-miR- hsa-miR- 7.35 6.85 5.75 6.65 0.8233 2.25 3.47 5.14 4.99 4.77 2.99 3.96 2.62 3.77 3.77 1.05 0  1.5E−032.88 7.3 409-3p 409-3p_st1 hsa-asg- hsa-asg- 6.89 6.99 6.66 6.85 0.17100 4.49 3.42 4.87 4.95 4.11 4.18 3.59 2.08 4.07 3.97 0.88 0  2.7E−042.88 7.3 13166 13166_st2 hsa-asg- hsa-asg- 8.22 7.74 8.06 8.01 0.25 1005.62 5.67 5.24 4.91 4.79 4.75 5.16 4.47 5.60 5.13 0.43 22  8.7E−07 2.887.3 13135 13135_st1 hsa-asg- hsa-asg- 12.0 12.2 11.84 12.0 0.19 100 10.99.01 9.13 11.2 9.75 8.53 10.2 8.11 5.85 9.20 1.64 100  1.5E−02 2.84 7.28878 8878_st2 hsa-miR- hsa-cand708_st1 7.74 7.45 7.86 7.69 0.21 100 5.945.23 4.02 6.58 5.46 4.24 5.64 3.07 3.53 4.86 1.19 44  2.6E−03 2.83 7.1365 hsa-miR- hsa-miR- 8.47 8.53 8.67 8.56 0.10 100 7.27 5.64 5.53 7.636.38 5.40 6.66 4.78 4.78 6.01 1.03 56  2.0E−03 2.55 5.8 365 365_st1hsa-miR- hsa-asg- 8.32 8.38 8.49 8.40 0.09 100 7.33 5.87 6.09 7.37 6.385.46 6.63 4.78 4.67 6.06 0.98 56  2.6E−03 2.33 5.0 365 13156_st1hsa-miR- hsa-miR- 6.49 6.39 7.09 6.66 0.38 100 3.93 2.87 3.67 3.63 3.852.85 3.85 4.68 5.18 3.84 0.75 0  1.1E−04 2.82 7.1 542-5p 542-5p_st2hsa-asg- hsa-asg- 5.97 5.35 5.38 5.57 0.35 100 3.33 3.02 2.18 3.57 2.532.17 2.92 3.13 1.86 2.75 0.59 0  1.6E−05 2.82 7.1 1826 1826_st2 hsa-miR-hsa-miR- 6.05 5.82 5.79 5.88 0.14 33 4.49 3.87 1.79 4.15 2.76 1.48 2.483.38 3.17 3.07 1.03 0  1.0E−03 2.82 7.0 149 149_st2 hsa-miR- hsa-miR-5.82 5.28 4.98 5.36 0.43 33 2.05 3.16 2.66 3.40 2.69 2.99 1.97 2.62 1.382.55 0.64 0  3.8E−05 2.81 7.0 539 539_st1 hsa-miR- hsa-miR- 6.17 7.157.60 6.97 0.73 100 4.81 4.86 4.87 4.76 4.00 2.69 4.14 2.93 4.40 4.160.83 0  4.0E−04 2.81 7.0 551b 551b_st1 hsa-miR- hsa- 6.43 6.96 7.59 6.990.58 100 5.25 4.43 3.79 5.85 3.39 4.03 4.82 3.54 4.32 4.38 0.81 22 4.7E−04 2.61 6.1 551b miR167_st2 hsa-miR- hsa-miR- 6.10 6.10 6.55 6.250.26 100 3.56 2.71 4.47 4.36 4.36 2.53 2.92 2.62 3.53 3.45 0.80 0 1.6E−04 2.80 7.0 369-5p 369-5p_st2 hsa-miR- hsa-asg- 6.56 6.17 5.826.18 0.37 100 3.76 3.42 4.02 2.23 3.72 3.73 3.40 2.78 3.53 3.40 0.56 0 1.2E−05 2.78 6.9 369-5p 1521_st1 hsa-cand26 hsa-cand26_st2 8.22 7.357.75 7.77 0.44 100 6.12 5.61 6.03 4.08 3.68 4.75 5.29 4.07 5.46 5.010.90 22  5.4E−04 2.76 6.8 hsa-asg- hsa-asg- 6.24 5.68 6.16 6.03 0.30 1003.07 2.54 4.81 2.42 2.53 2.77 3.17 3.91 4.16 3.27 0.84 0  2.9E−04 2.766.8 1296 1296_st1 hsa-asg- hsa-asg- 5.89 6.14 5.24 5.76 0.47 100 3.762.87 2.66 3.40 3.49 2.85 2.92 2.93 2.10 3.00 0.49 0  7.1E−06 2.76 6.812690 12690_st1 hsa-miR- hsa-miR- 5.38 6.01 6.16 5.85 0.41 67 4.09 2.873.79 2.77 2.19 3.54 3.68 2.62 2.33 3.10 0.69 0  7.7E−05 2.75 6.7 641641_st1 hsa- hsa- 4.32 4.89 6.26 5.16 0.99 33 3.45 2.71 1.66 3.15 1.111.58 3.77 1.88 2.54 2.43 0.92 0  1.4E−03 2.73 6.6 cand317 cand317_st1hsa-miR- hsa- 6.27 6.67 5.81 6.25 0.43 100 3.93 2.54 1.92 5.43 5.30 2.533.17 3.20 3.65 3.52 1.21 0  3.9E−03 2.73 6.6 193a cand178_st1hsa-asg-3822 hsa-asg- 5.63 5.18 5.56 5.46 0.24 33 2.05 3.02 3.67 2.032.45 2.17 1.60 2.93 4.78 2.75 0.99 0  1.0E−03 2.71 6.6 3822_st2 hsa-miR-hsa-miR- 5.24 4.13 5.73 5.03 0.82 67 2.25 2.87 3.06 1.72 2.01 1.97 1.972.08 3.09 2.34 0.53 0  4.8E−05 2.70 6.5 576 576_st2 hsa-cand206hsa-cand206_st1 5.82 4.89 5.40 5.37 0.47 67 2.25 1.96 3.79 3.63 2.692.17 3.59 1.29 2.74 2.68 0.86 0  4.7E−04 2.69 6.5 hsa-miR- hsa-miR- 9.449.18 9.21 9.28 0.15 100 6.52 6.42 7.11 6.97 7.04 6.59 6.35 6.14 6.236.60 0.36 100  2.4E−07 2.68 6.4 379 379_st1 hsa-miR-1 hsa-miR- 12.6 12.712.60 12.6 0.07 100 11.0 10.8 8.18 11.7 10.7 10.0 10.1 7.66 9.47 9.991.35 100  7.8E−03 2.68 6.4 1_st1 hsa-miR-200a* hsa-miR- 5.53 4.73 5.975.41 0.63 67 3.07 2.87 2.18 3.91 2.61 1.78 2.15 3.20 2.92 2.74 0.65 0 9.9E−05 2.67 6.3 200a-_st2 hsa-miR- hsa- 7.35 7.30 8.03 7.56 0.41 1005.45 4.81 5.37 6.66 4.11 4.43 4.82 4.58 3.87 4.90 0.84 22  4.2E−04 2.666.3 499 miR12_st1 hsa-miR- hsa-miR- 6.79 6.91 7.84 7.18 0.58 100 5.324.66 4.75 6.58 4.53 4.18 5.05 4.28 4.60 4.88 0.73 22  6.0E−04 2.30 4.9499 499_st1 hsa-miR- hsa-miR- 5.28 5.16 6.05 5.50 0.49 67 4.61 3.42 2.863.15 3.63 2.53 1.60 1.29 2.54 2.85 1.02 0  1.7E−03 2.65 6.3 19a 19a_st1hsa-miR- hsa-miR- 6.16 6.28 5.80 6.08 0.25 100 3.76 3.02 4.61 3.28 4.582.85 2.78 3.32 2.74 3.44 0.73 0  1.3E−04 2.64 6.2 329 329_st1 hsa-miR-hsa-asg- 6.20 6.19 6.18 6.19 0.01 100 4.09 2.87 4.21 3.40 4.24 3.48 4.002.62 3.53 3.61 0.59 0  2.3E−05 2.58 6.0 329 13204_st2 hsa-asg- hsa-asg-4.97 4.94 5.45 5.12 0.29 33 3.33 2.71 1.92 2.33 3.16 2.35 2.64 1.88 2.102.49 0.51 0  9.0E−06 2.63 6.2 9253 9253_st2 hsa-miR- hsa-miR- 11.5 11.711.59 11.6 0.12 100 9.40 9.08 9.04 10.6 10.3 8.25 9.05 6.55 8.85 9.031.19 100  4.2E−03 2.62 6.1 199b 199b_st2 hsa-asg- hsa-asg- 9.17 8.729.39 9.10 0.34 100 7.43 6.36 6.67 7.04 6.26 6.27 6.63 6.55 5.09 6.480.64 89  6.1E−05 2.62 6.1 9696 9696_st1 hsa-miR- hsa-miR- 9.15 8.87 9.739.25 0.44 100 6.89 7.22 7.37 6.61 6.11 6.50 6.41 6.62 6.01 6.64 0.46 78 6.2E−06 2.61 6.1 503 503_st1 hsa-miR- hsa- 9.02 8.66 9.60 9.09 0.47 1007.11 7.33 7.30 6.52 6.57 6.68 6.86 6.89 6.76 6.89 0.30 100  2.0E−06 2.204.6 503 miR141_st1 hsa-asg- hsa-asg- 8.91 8.60 8.71 8.74 0.15 100 7.466.04 7.10 7.36 4.72 5.57 6.22 7.09 3.77 6.15 1.28 78  6.7E−03 2.59 6.013230 13230_st2 hsa-asg- hsa-asg- 11.2 11.2 11.57 11.3 0.20 100 8.638.58 8.70 9.41 8.77 8.37 9.21 8.76 8.49 8.77 0.34 100  2.4E−07 2.57 5.93998 3998_st1 hsa-miR- hsa-miR- 8.28 7.67 8.63 8.19 0.49 100 6.71 5.985.89 6.87 5.97 5.59 5.59 3.07 5.05 5.64 1.11 56  3.7E−03 2.56 5.9 598598_st2 hsa-asg- hsa-asg- 8.34 7.89 7.90 8.04 0.26 100 6.06 5.72 5.286.77 6.26 5.43 5.46 4.21 4.16 5.48 0.87 44  6.5E−04 2.56 5.9 1417614176_st1 hsa-asg- hsa-asg- 5.20 4.73 4.78 4.90 0.26 33 2.78 2.36 2.662.33 1.64 2.17 1.60 2.08 3.53 2.35 0.59 0  3.5E−05 2.55 5.9 1213912139_st2 hsa-miR-9* hsa-miR-9-_st2 8.31 7.86 8.19 8.12 0.23 100 6.206.10 5.57 6.11 5.41 5.87 4.94 4.73 5.23 5.57 0.54 44  1.4E−05 2.55 5.9hsa-miR-9 hsa-miR- 6.18 5.65 5.78 5.87 0.28 100 3.45 3.53 2.86 3.28 3.632.17 3.50 3.32 4.16 3.32 0.55 0  2.0E−05 2.55 5.8 9_st2 hsa-asg-hsa-asg- 7.03 6.99 7.44 7.16 0.25 100 4.85 4.76 4.54 5.12 4.55 4.18 4.984.35 4.16 4.61 0.34 0  3.8E−07 2.55 5.8 128 128_st2 hsa-asg- hsa-asg-5.28 4.79 5.32 5.13 0.30 67 2.25 2.16 2.86 3.63 2.37 2.35 3.29 2.27 2.102.59 0.55 0  2.0E−05 2.54 5.8 1765 1765_st1 hsa-miR- hsa-miR- 5.60 5.204.38 5.06 0.62 33 2.25 2.87 2.66 3.40 2.19 3.26 0.91 2.27 2.92 2.53 0.750  3.6E−04 2.54 5.8 487a 487a_st2 hsa-miR- hsa-asg- 6.31 5.56 5.12 5.660.60 100 4.09 3.64 3.91 3.40 3.89 3.78 3.68 3.74 3.65 3.75 0.20 0 4.5E−06 1.91 3.8 487a 6915_st1 hsa-asg- hsa-asg- 11.8 11.8 12.16 11.90.18 100 10.5 9.46 9.58 9.81 9.17 8.63 9.51 8.96 9.11 9.43 0.56 100 2.1E−05 2.52 5.8 13308 13308_st2 hsa-miR- hsa-miR- 11.7 11.5 11.84 11.70.14 100 9.10 9.02 9.17 9.80 8.98 8.85 9.69 9.24 9.14 9.22 0.32 100 1.6E−07 2.49 5.6 422a 422a_st1 hsa-asg- hsa-asg- 11.9 11.8 12.23 12.00.18 100 10.6 9.48 9.75 10.1 9.40 8.81 9.58 9.07 9.15 9.56 0.56 100 2.4E−05 2.47 5.6 5304 5304_st1 hsa-miR- hsa-miR- 11.5 11.5 11.47 11.50.05 100 9.49 8.88 8.87 10.3 10.1 8.31 9.92 7.23 8.38 9.07 1.01 100 2.2E−03 2.46 5.5 193b 193b_st1 hsa-asg-10881 hsa-asg- 7.84 7.29 7.807.64 0.31 100 5.73 5.04 6.06 5.43 4.79 5.46 4.20 5.04 5.23 5.22 0.54 22 2.8E−05 2.42 5.4 10881_st1 hsa-asg- hsa-asg- 5.48 5.62 6.42 5.84 0.51100 2.78 3.53 3.39 3.28 3.63 3.37 3.85 2.62 4.32 3.42 0.52 0  3.4E−052.42 5.4 11361 11361_st2 hsa-miR- hsa-miR- 11.6 11.1 11.49 11.4 0.26 1009.35 8.42 9.39 10.2 9.96 8.12 9.20 8.06 8.47 9.02 0.80 100  5.2E−04 2.415.3 574 574_st1 hsa-miR- hsa-miR- 11.0 10.7 10.40 10.7 0.31 100 9.728.56 8.33 8.35 8.59 8.08 8.02 8.44 6.88 8.33 0.74 89  3.1E−04 2.40 5.3132 132_st1 hsa-asg- hsa-asg- 4.90 4.56 5.32 4.92 0.38 33 2.43 2.71 1.921.93 2.01 2.53 2.92 2.62 3.65 2.53 0.55 0  4.3E−05 2.40 5.3 51195119_st1 hsa-miR- hsa-miR- 5.81 5.03 5.77 5.54 0.44 67 4.49 2.36 3.673.52 3.68 2.85 1.60 3.32 2.92 3.16 0.84 0  1.0E−03 2.38 5.2 589 589_st1hsa-miR- hsa-asg- 5.51 5.75 5.73 5.66 0.13 33 4.99 3.92 2.86 5.14 4.602.99 4.00 3.64 3.77 3.99 0.80 0  5.7E−03 1.67 3.2 589 9674_st1 hsa-asg-hsa-asg- 6.31 5.87 6.71 6.29 0.42 100 5.18 4.49 4.02 1.72 3.39 2.85 3.855.08 4.67 3.92 1.12 11  5.8E−03 2.38 5.2 9980 9980_st1 hsa-miR- hsa-miR-7.12 6.59 7.48 7.07 0.45 100 5.83 4.99 5.24 5.71 4.89 4.46 4.49 3.992.74 4.71 0.94 11  2.2E−03 2.36 5.1 340 340_st1 hsa-asg- hsa-asg- 7.047.17 7.06 7.09 0.07 100 5.92 4.49 5.04 6.08 5.09 4.87 4.14 2.93 4.074.73 0.97 22  2.2E−03 2.35 5.1 8411 8411_st2 hsa-miR- hsa-miR- 13.0 13.013.06 13.0 0.02 100 10.6 10.5 10.7 11.3 10.7 10.3 10.9 10.7 10.5 10.70.27 100  5.1E−08 2.33 5.0 422b 422b_st2 hsa-asg-10883 hsa-asg- 9.379.49 9.46 9.44 0.07 100 7.64 7.18 7.34 8.22 8.03 6.58 6.97 5.74 6.637.15 0.78 89  5.8E−04 2.29 4.9 10883_st1 hsa-miR- hsa-miR- 5.12 6.416.69 6.07 0.84 67 4.76 4.66 3.39 5.42 4.97 2.92 2.32 2.27 3.39 3.79 1.190  1.2E−02 2.28 4.9 452* 452-_st1 hsa-asg- hsa-asg- 4.01 3.35 5.43 4.261.07 33 1.65 0.78 2.18 1.21 1.92 1.97 2.78 2.62 2.74 1.98 0.69 0 1.3E−03 2.28 4.9 12227 12227_st1 hsa-asg- hsa-asg- 5.50 5.03 5.95 5.490.46 67 3.33 4.08 2.86 3.01 2.90 3.26 2.78 2.78 4.16 3.24 0.53 0 6.9E−05 2.25 4.8 8174 8174_st1 hsa-asg- hsa-asg- 4.69 5.39 4.44 4.840.49 33 2.43 2.36 1.39 4.00 2.19 2.35 3.40 2.78 2.54 2.60 0.74 0 7.1E−04 2.24 4.7 2484 2484_st2 hsa-miR- hsa-miR- 6.62 4.70 6.98 6.101.23 67 4.37 3.83 5.04 4.40 4.83 3.48 4.33 2.45 2.10 3.87 1.02 0 1.0E−02 2.23 4.7 585 585_st1 hsa-asg- hsa-asg-13279_st1 9.38 9.14 9.819.44 0.34 100 7.58 7.47 7.55 8.17 7.24 6.59 6.87 7.00 6.68 7.24 0.51 100 4.1E−05 2.21 4.6 13279 hsa-miR- hsa-miR- 12.7 12.5 12.87 12.7 0.15 10011.0 10.5 10.5 11.4 11.1 10.1 10.1 9.52 10.3 10.5 0.59 100  1.0E−04 2.204.6 99b 99b_st1 hsa-miR- hsa-asg- 12.8 12.7 12.93 12.8 0.11 100 11.210.6 10.6 11.6 11.3 10.3 10.3 9.55 10.5 10.7 0.65 100  2.8E−04 2.11 4.399b 14196_st2 hsa-asg- hsa-asg- 5.77 5.71 6.65 6.04 0.53 100 4.31 3.744.39 4.58 3.33 3.48 2.78 3.95 4.07 3.85 0.57 0  1.6E−04 2.20 4.6 1423014230_st1 hsa-asg- hsa-asg- 5.35 4.49 4.64 4.83 0.46 33 2.05 2.54 3.672.51 1.46 2.85 2.64 2.45 3.77 2.66 0.72 0  7.1E−04 2.17 4.5 1390813908_st2 hsa-asg- hsa-asg- 8.02 8.13 8.42 8.19 0.21 100 7.66 6.59 4.478.11 7.31 4.95 6.96 3.91 4.32 6.03 1.61 56  4.9E−02 2.16 4.5 1309713097_st1 hsa-miR- hsa-miR- 8.69 9.33 9.50 9.17 0.42 100 7.43 7.60 6.238.75 7.86 6.49 6.51 5.62 6.69 7.02 0.96 33  4.4E−03 2.15 4.4 497 497_st1hsa-miR- hsa- 9.81 10.4 10.37 10.2 0.34 100 8.20 8.56 7.51 9.37 8.887.78 7.45 7.13 7.83 8.08 0.74 89  8.1E−04 2.12 4.4 497 miR43_st1hsa-asg- hsa-asg- 6.08 5.42 6.05 5.85 0.37 67 4.24 3.29 4.68 3.46 3.443.32 3.77 3.83 3.39 3.71 0.47 0  3.5E−05 2.14 4.4 14155 14155_st2hsa-asg- hsa-asg- 4.52 4.28 5.78 4.86 0.80 33 2.25 2.71 2.18 4.71 2.832.99 1.08 2.78 3.09 2.74 0.96 0  6.6E−03 2.12 4.4 378 378_st1 hsa-miR-hsa-miR- 7.20 7.43 8.44 7.69 0.66 100 5.11 5.30 6.01 6.29 5.74 5.68 5.405.90 4.73 5.57 0.49 78  1.2E−04 2.12 4.3 542-3p 542-3p_st1 hsa-asg-11443hsa-asg- 4.99 5.08 5.64 5.23 0.35 67 4.17 3.64 2.86 2.69 3.54 2.69 3.402.78 2.33 3.12 0.59 0  1.8E−04 2.11 4.3 11443_st1 hsa-miR- hsa-miR- 11.411.5 11.78 11.5 0.16 100 9.91 9.64 10.0 10.0 9.43 8.17 9.21 9.10 9.769.48 0.59 100  1.5E−04 2.11 4.3 106b 106b_st2 hsa-miR- hsa-miR- 6.286.50 6.55 6.44 0.15 100 5.83 3.53 4.21 4.99 4.63 3.95 4.27 3.20 4.404.33 0.78 11  1.1E−03 2.11 4.3 154* 154-_st2 hsa-miR- hsa-miR- 10.0 10.010.12 10.0 0.06 100 7.92 8.13 8.33 8.27 7.80 7.42 7.82 7.87 7.98 7.950.27 100  1.5E−07 2.11 4.3 324-5p 324-5p_st1 hsa-miR- hsa-miR- 7.48 7.656.76 7.30 0.47 100 5.11 5.78 5.99 5.85 5.70 4.21 4.73 4.31 5.02 5.190.68 0  5.9E−04 2.11 4.3 134 134_st2 hsa-cand852 hsa-cand852_st2 5.533.54 5.33 4.80 1.10 67 3.07 1.96 2.86 3.01 2.28 2.69 2.92 2.08 3.53 2.710.51 0  8.9E−04 2.09 4.2 hsa-asg-14233 hsa-asg- 11.4 11.1 11.36 11.30.13 100 9.45 9.26 9.23 10.1 9.44 8.89 9.05 8.87 8.89 9.25 0.41 100 7.3E−06 2.07 4.2 14233_st1 hsa-miR- hsa-asg- 9.96 10.1 10.36 10.1 0.20100 8.63 8.31 8.53 8.45 7.87 7.92 7.91 7.33 7.81 8.08 0.42 100  1.2E−052.07 4.2 454-3p 11541_st2 hsa-miR- hsa-asg- 10.6 10.5 10.86 10.6 0.19100 9.63 8.98 9.24 9.13 8.55 8.77 8.57 7.82 8.33 8.78 0.54 100  1.8E−041.88 3.7 454-3p 13305_st1 hsa-miR- hsa-asg- 5.22 4.28 5.04 4.85 0.50 333.66 3.42 3.23 1.93 2.45 1.97 1.60 3.20 3.53 2.78 0.79 0  1.8E−03 2.074.2 299-3p 10378_st1 hsa-miR- hsa-miR- 9.11 8.82 8.40 8.78 0.36 100 7.176.85 7.35 7.13 7.43 6.56 6.29 5.93 5.81 6.72 0.61 78  2.8E−04 2.05 4.2382 382_st1 hsa-asg- hsa-asg-9687_st1 6.18 6.12 6.36 6.22 0.12 100 4.764.81 3.91 4.66 3.89 4.24 4.07 3.74 3.53 4.18 0.47 0  2.7E−05 2.04 4.19687 hsa-miR- hsa-asg- 7.67 7.32 6.99 7.32 0.34 100 5.89 5.28 6.15 5.275.33 4.91 5.12 5.46 4.20 5.29 0.56 0  1.6E−04 2.03 4.1 330 14199_st1hsa-miR- hsa-miR- 7.51 6.96 6.98 7.15 0.31 100 6.08 5.71 5.82 4.26 5.255.20 5.35 5.06 4.50 5.25 0.59 22  4.0E−04 1.90 3.7 330 330_st2 hsa-miR-hsa-miR- 6.02 6.04 4.30 5.45 1.00 33 4.66 3.10 4.07 3.01 2.28 1.88 4.333.49 4.11 3.43 0.95 0  1.0E−02 2.02 4.0 493-3p 493-3p_st1 hsa-asg-hsa-asg- 3.62 4.49 4.86 4.33 0.64 33 1.65 1.75 3.06 1.31 2.01 2.99 3.292.45 2.33 2.31 0.69 0 1.2E−03 2.01 4.0 2367 2367_st1 hsa-miR-30e-5phsa-miR-30e-5p_st2 11.4 11.4 11.87 11.5 0.26 100 10.4 9.64 9.71 10.59.81 9.23 9.23 8.15 9.50 9.58 0.70 100  8.2E−04 2.00 4.0 hsa-miR-hsa-miR- 13.2 13.0 13.29 13.1 0.14 100 12.0 11.7 10.9 12.2 11.6 11.110.8 9.75 10.5 11.2 0.79 100  1.7E−03 1.99 4.0 152 152_st1 hsa-asg-hsa-asg- 8.26 8.51 8.15 8.31 0.19 100 7.36 6.37 6.19 6.97 6.35 5.27 5.825.74 6.88 6.33 0.66 89  5.7E−04 1.98 3.9 925 925_st1 hsa-asg- hsa-asg-5.33 5.16 5.83 5.44 0.35 67 4.09 3.02 3.67 4.76 3.39 2.69 3.17 3.32 3.093.47 0.63 0  4.8E−04 1.97 3.9 10679 10679_st2 hsa-asg- hsa-asg- 8.148.23 8.31 8.23 0.09 100 6.57 6.36 6.43 7.06 6.10 6.13 5.59 5.93 6.256.27 0.42 89  1.3E−05 1.96 3.9 14175 14175_st1 hsa- hsa- 10.6 10.6 11.0410.7 0.22 100 9.62 9.10 9.23 9.55 8.87 8.54 8.50 8.15 7.91 8.83 0.60 100 3.0E−04 1.96 3.9 miR257 miR257_st1 hsa-miR- hsa-miR- 11.7 11.5 11.3311.5 0.23 100 9.76 9.55 9.76 10.9 10.3 9.93 9.27 8.44 8.52 9.62 0.81 100 2.5E−03 1.95 3.9 214 214_st1 hsa-miR- hsa-miR- 9.17 9.23 9.52 9.30 0.19100 7.66 7.30 7.35 8.09 7.56 6.92 7.41 6.65 7.27 7.36 0.41 0  1.6E−051.95 3.9 324-3p 324-3p_st1 hsa-miR- hsa-miR- 7.89 9.24 8.93 8.69 0.70100 7.92 6.71 6.74 8.78 7.67 5.74 7.42 4.41 5.39 6.75 1.38 67  4.5E−021.93 3.8 452 452_st1 hsa-miR- hsa-miR- 9.09 9.41 9.71 9.40 0.31 100 8.388.32 7.31 8.03 8.04 6.51 6.91 6.53 7.20 7.47 0.74 100  1.6E−03 1.93 3.819b 19b_st2 hsa-miR- hsa-miR- 8.10 8.18 8.30 8.19 0.10 100 6.59 6.676.42 6.87 6.43 6.02 5.86 6.26 5.29 6.27 0.48 89  5.7E−05 1.93 3.8 425-3p425_st2 hsa-miR- hsa-miR- 8.78 8.59 8.45 8.61 0.17 100 6.40 7.30 7.226.96 6.63 7.66 5.73 5.86 6.60 6.71 0.65 100  6.5E−04 1.90 3.7 494494_st1 hsa-miR- hsa- 7.97 7.80 8.07 7.94 0.14 100 6.83 6.55 6.85 6.436.38 7.10 5.40 5.79 6.89 6.47 0.55 89  1.2E−03 1.48 2.8 494 miR61_st2hsa-asg- hsa-asg- 5.89 4.98 5.42 5.43 0.45 33 4.02 3.23 3.96 4.46 3.332.17 2.48 4.11 4.02 3.53 0.79 0  3.0E−03 1.90 3.7 1707 1707_st2 hsa-miR-hsa-miR- 11.3 11.0 11.34 11.2 0.18 100 10.7 9.36 9.71 9.95 8.86 8.999.01 8.77 8.68 9.35 0.69 100  1.0E−03 1.88 3.7 128a 128a_st2 hsa-asg-hsa-asg- 6.20 5.72 6.42 6.11 0.36 67 4.43 5.12 4.02 3.21 4.39 4.18 4.144.21 4.40 4.23 0.50 0  1.3E−04 1.88 3.7 1596 1596_st2 hsa-miR- hsa-miR-6.41 6.48 6.57 6.49 0.08 100 5.29 4.40 5.55 5.86 5.01 3.78 4.27 4.033.32 4.61 0.86 11  4.3E−03 1.88 3.7 296 296_st1 hsa-miR-28 hsa-miR- 12.412.1 12.38 12.3 0.12 100 11.1 10.7 10.0 11.0 10.8 10.1 10.1 9.40 10.510.4 0.57 100  2.7E−04 1.87 3.6 28_st1 hsa-asg-13888 hsa-asg- 4.01 4.214.14 4.12 0.10 33 1.45 2.87 1.66 3.15 1.83 2.17 2.64 1.88 2.74 2.26 0.600  4.3E−04 1.86 3.6 13888_st2 hsa-cand581 hsa-cand581_st1 5.28 5.12 5.495.29 0.18 67 3.85 3.64 3.67 3.91 3.72 2.69 3.85 2.45 3.25 3.45 0.54 0 2.0E−04 1.85 3.6 hsa-miR- hsa-miR- 11.3 11.5 11.83 11.5 0.26 100 10.49.56 9.54 10.2 9.75 9.33 9.52 9.38 9.86 9.73 0.37 100  1.3E−05 1.85 3.6222 222_st2 hsa-asg- hsa-asg- 5.03 5.46 5.84 5.44 0.40 67 3.45 3.53 3.234.36 3.81 3.48 4.14 3.74 2.74 3.61 0.48 0  1.5E−04 1.84 3.6 41404140_st2 hsa-miR- hsa-miR- 8.47 8.35 8.39 8.40 0.06 100 7.01 5.98 6.518.11 7.01 5.65 6.50 5.99 6.37 6.57 0.74 100  1.9E−03 1.83 3.6 374374_st2 hsa-miR- hsa-miR- 5.86 6.23 6.87 6.32 0.51 67 4.31 4.79 2.545.96 5.84 4.28 3.85 3.59 5.29 4.49 1.11 0  2.4E−02 1.83 3.5 504 504_st1hsa-miR- hsa-miR- 7.31 7.23 7.50 7.35 0.14 100 5.78 6.02 7.10 5.97 4.534.14 5.69 5.67 4.89 5.53 0.89 44  6.7E−03 1.81 3.5 18a 18a_st1hsa-cand207 hsa-cand207_st1 4.92 5.46 4.71 5.03 0.38 33 2.61 4.16 3.672.51 3.68 3.69 2.64 2.78 3.25 3.22 0.60 0  7.3E−04 1.81 3.5 hsa-asg-hsa-asg- 4.46 4.49 5.22 4.72 0.43 33 2.43 3.29 3.54 3.28 2.69 1.97 3.502.62 2.92 2.92 0.53 0  3.5E−04 1.81 3.5 9702 9702_st2 hsa-miR- hsa-miR-8.56 8.66 9.24 8.82 0.37 100 7.60 6.47 7.28 8.05 7.76 6.27 6.60 6.186.88 7.01 0.69 100  1.6E−03 1.81 3.5 186 186_st1 hsa-miR- hsa-miR- 6.787.31 7.86 7.32 0.54 100 6.66 5.97 5.21 6.67 5.29 5.13 5.02 4.25 5.445.51 0.79 33  4.7E−03 1.80 3.5 339 339_st1 hsa-miR- hsa-miR- 7.60 8.077.85 7.84 0.23 100 7.73 6.16 6.37 7.22 6.63 5.50 4.78 4.78 5.27 6.051.05 44  1.7E−02 1.79 3.5 140 140_st2 hsa-miR- hsa-asg- 7.41 8.24 8.077.90 0.44 100 7.75 7.03 6.53 7.45 6.62 5.05 5.71 3.83 5.23 6.13 1.28 67 4.4E−02 1.77 3.4 140 14239_st1 hsa-miR- hsa-miR- 6.57 6.75 6.71 6.680.09 100 5.73 4.76 3.06 5.53 6.52 4.49 5.88 4.35 3.65 4.88 1.13 33 2.3E−02 1.79 3.5 193a 193a_st1 hsa-asg- hsa-asg- 6.04 5.65 6.16 5.950.27 100 4.85 4.30 4.98 4.76 3.72 2.85 4.07 3.32 4.54 4.16 0.73 0 2.4E−03 1.79 3.5 9681 9681_st1 hsa-miR- hsa-miR- 11.6 11.3 11.64 11.50.16 100 11.2 9.83 10.0 10.3 9.19 9.33 9.54 9.19 9.25 9.77 0.69 100 1.5E−03 1.78 3.4 128b 128b_st1 hsa-asg- hsa-asg- 6.65 6.71 6.96 6.780.17 100 4.71 6.29 6.06 5.88 5.25 5.56 4.44 2.27 4.47 4.99 1.23 11 3.5E−02 1.78 3.4 2924 2924_st1 hsa-miR- hsa-miR- 7.47 7.05 7.31 7.280.21 33 5.25 6.17 5.04 5.61 5.54 5.75 5.14 5.51 5.46 5.50 0.34 0 8.1E−06 1.78 3.4 491 491_st1 hsa-asg-10360 hsa-asg- 5.08 5.08 5.09 5.080.01 33 3.45 3.42 3.06 4.66 3.72 3.26 3.17 2.45 2.74 3.32 0.63 0 8.7E−04 1.76 3.4 10360_st1 hsa-asg- hsa-asg- 5.66 5.94 6.50 6.04 0.4367 4.49 3.53 4.02 5.45 4.77 3.64 4.00 4.35 4.32 4.28 0.59 0  8.6E−041.75 3.4 360 360_st2 hsa-miR- hsa-miR- 9.43 9.12 9.51 9.35 0.20 100 8.307.88 7.20 8.00 7.35 7.52 7.24 7.66 7.44 7.62 0.37 100  2.0E−05 1.73 3.3628 628_st2 hsa-asg- hsa-asg- 6.82 6.21 6.73 6.59 0.33 100 5.78 4.585.35 5.50 4.91 5.32 4.14 3.95 4.57 4.90 0.63 0 1.4E−03 1.69 3.2 1413814138_st2 hsa-miR- hsa-miR- 13.9 14.0 13.89 13.9 0.07 100 12.5 12.3 12.013.2 12.8 11.9 11.9 11.0 12.5 12.2 0.64 100 1.3E−03 1.68 3.2 27b 27b_st1hsa- hsa- 6.10 5.16 4.21 5.16 0.95 33 3.85 2.71 4.93 3.95 3.63 2.44 4.272.45 3.09 3.48 0.87 0  1.7E−02 1.67 3.2 miR202 miR202_st2 hsa-miR-hsa-miR- 12.7 12.7 12.71 12.7 0.04 100 11.3 11.0 11.0 12.0 11.5 10.610.6 10.2 11.1 11.0 0.54 100  4.2E−04 1.67 3.2 27a 27a_st2 hsa- hsa-5.16 5.16 5.05 5.12 0.06 67 3.76 3.02 3.23 4.00 4.24 3.37 2.64 3.44 3.393.45 0.49 0  1.9E−04 1.67 3.2 cand821 cand821_st1 hsa-miR- hsa-miR- 7.297.21 6.59 7.03 0.38 100 6.27 5.73 6.17 5.72 5.17 5.11 4.59 4.98 4.765.39 0.61 11  1.5E−03 1.64 3.1 212 212_st2 hsa-miR- hsa-miR- 7.48 7.396.98 7.28 0.27 100 5.14 5.04 6.67 6.09 6.11 5.17 5.92 5.45 5.27 5.650.57 44  8.4E−04 1.63 3.1 409-5p 409-5p_st1 hsa-miR- hsa-miR- 9.72 9.649.72 9.69 0.05 100 9.76 7.76 8.97 9.69 7.39 7.36 7.88 7.63 6.14 8.061.19 89  4.4E−02 1.63 3.1 505 505_st1 hsa-asg- hsa-asg- 6.18 6.79 6.546.51 0.31 100 4.66 4.37 4.02 6.18 5.09 4.21 5.16 5.22 5.05 4.88 0.66 11 2.3E−03 1.62 3.1 7465 7465_st2 hsa-asg- hsa-asg- 8.16 8.28 7.69 8.040.31 100 6.62 6.49 6.49 7.56 7.45 5.68 6.35 5.00 6.23 6.43 0.79 67 7.3E−03 1.62 3.1 13304 13304_st2 hsa-miR- hsa- 5.56 4.79 4.30 4.88 0.6433 4.31 2.54 4.12 3.28 2.45 3.26 3.59 2.78 3.09 3.27 0.65 0  3.8E−031.61 3.1 485-3p miR23_st2 hsa-miR- hsa-miR- 5.65 5.52 4.88 5.35 0.41 673.66 3.02 4.30 4.43 4.27 3.13 3.68 3.54 3.87 3.77 0.50 0  6.2E−04 1.583.0 485-3p 485-3p_st2 hsa-miR- hsa-miR- 4.90 5.35 4.90 5.05 0.26 67 3.663.53 3.39 4.71 3.44 3.37 4.07 1.88 2.92 3.44 0.77 0  6.2E−03 1.61 3.1599 599_st2 hsa-asg- hsa-asg- 3.62 4.04 4.78 4.15 0.58 33 3.07 3.29 2.183.01 2.19 2.35 1.25 3.20 2.33 2.54 0.66 0  3.9E−03 1.61 3.0 1399713997_st2 hsa-asg-14133 hsa-asg- 8.58 8.63 8.93 8.71 0.19 100 8.97 6.977.91 7.93 6.95 6.01 7.09 5.86 6.31 7.11 1.01 89  2.4E−02 1.60 3.014133_st2 hsa-miR- hsa-miR- 10.6 10.7 10.85 10.7 0.08 100 10.0 8.79 9.4410.0 9.63 8.27 9.09 8.14 9.14 9.18 0.70 100  3.2E−03 1.60 3.0 148b148b_st2 hsa-miR- hsa-miR- 7.25 6.97 7.38 7.20 0.21 100 6.18 5.61 6.716.01 5.09 4.68 5.71 4.87 5.60 5.61 0.65 44  2.2E−03 1.60 3.0 18b 18b_st1hsa-miR- hsa-miR- 10.8 10.7 10.46 10.7 0.21 100 10.5 9.57 9.77 9.80 8.729.01 9.09 7.97 7.54 9.11 0.94 100  1.7E−02 1.59 3.0 423 423_st1 hsa-asg-hsa-asg- 4.61 5.59 6.36 5.52 0.88 33 4.55 3.36 4.50 5.28 4.04 3.26 3.593.59 3.17 3.93 0.72 0  1.0E−02 1.59 3.0 7417 7417_st1 hsa-asg- hsa-asg-6.20 6.36 6.11 6.22 0.13 100 5.03 4.55 4.54 5.96 4.85 4.49 4.33 3.994.16 4.66 0.58 22  1.1E−03 1.57 3.0 13011 13011_st2 hsa-miR- hsa-miR-11.0 10.8 10.96 10.9 0.09 100 10.2 9.76 9.14 9.80 9.38 9.47 9.04 8.519.05 9.37 0.50 100  4.6E−04 1.55 2.9 151 151_st2 hsa-miR-hsa-cand144_st1 11.6 11.7 11.63 11.6 0.04 100 10.4 9.85 9.72 10.9 10.89.26 10.0 9.56 10.6 10.1 0.59 100  1.3E−03 1.54 2.9 130a hsa-asg-hsa-asg- 7.78 7.66 7.03 7.49 0.40 67 6.42 6.07 5.71 6.89 6.85 4.84 5.335.29 6.16 5.95 0.71 0  5.9E−03 1.54 2.9 279 279_st2 hsa-miR- hsa-miR-10.7 11.3 10.68 10.9 0.38 100 10.9 10.3 8.69 10.0 9.47 9.34 9.58 7.339.03 9.42 1.03 100  3.4E−02 1.54 2.9 335 335_st1 hsa-asg- hsa-asg- 4.695.52 4.54 4.92 0.53 33 3.76 4.61 3.79 3.28 4.24 2.99 3.85 1.48 2.74 3.420.93 0 2.61E−02 1.50 2.8 14238 14238_st1 hsa-asg- hsa-asg- 4.32 5.495.66 5.16 0.73 67 4.24 3.64 1.92 4.08 4.58 4.89 3.05 3.32 3.25 3.66 0.900 2.77E−02 1.50 2.8 1270 1270_st2 hsa-asg- hsa-asg- 4.64 3.95 5.09 4.560.57 33 3.07 1.96 3.54 3.52 2.83 1.97 3.40 2.78 4.60 3.07 0.83 01.72E−02 1.49 2.8 548 548_st1 hsa-miR- hsa-miR- 9.06 7.68 8.69 8.47 0.71100 7.66 6.76 6.71 7.93 7.56 5.83 7.52 6.32 6.65 6.99 0.70 89 1.04E−021.48 2.8 183 183_st1 hsa-asg- hsa-asg- 4.39 4.36 5.19 4.65 0.47 33 3.933.74 2.86 3.73 3.22 1.78 4.00 2.45 2.92 3.18 0.75 0 1.09E−02 1.46 2.813819 13819_st1 hsa-asg- hsa-asg- 9.61 9.58 9.59 9.59 0.01 100 8.46 7.838.45 8.85 8.22 7.49 8.10 7.55 8.21 8.13 0.45 78 2.56E−04 1.46 2.8 76437643_st2 hsa-miR- hsa-miR- 11.4 11.6 11.63 11.5 0.08 100 10.3 9.96 9.7110.9 10.7 9.21 10.0 9.63 10.4 10.1 0.56 100 1.49E−03 1.45 2.7 130a130a_st1 hsa-asg- hsa-asg- 7.41 7.76 7.13 7.44 0.32 100 6.68 6.40 5.826.62 6.67 5.74 5.32 5.31 5.40 6.00 0.60 33 2.95E−03 1.44 2.7 80678067_st2 hsa-asg- hsa-asg- 4.61 4.49 5.55 4.88 0.58 33 3.45 4.49 1.664.55 3.59 4.24 3.85 2.08 3.09 3.44 1.02 0 4.61E−02 1.44 2.7 86168616_st2 hsa-asg- hsa-asg- 4.49 4.43 5.36 4.76 0.52 33 4.37 3.29 2.864.81 3.16 2.85 2.32 3.07 3.25 3.33 0.78 0 1.56E−02 1.43 2.7 16191619_st2 hsa-asg- hsa-asg- 5.35 3.28 3.18 3.93 1.22 33 2.25 3.42 2.183.15 2.01 2.35 1.79 2.93 2.54 2.51 0.54 0 1.55E−02 1.42 2.7 57215721_st2 hsa-asg- hsa-asg- 5.38 4.76 5.46 5.20 0.38 33 4.90 2.87 4.024.26 3.93 2.69 3.68 3.44 4.24 3.78 0.70 0 8.24E−03 1.42 2.7 1296412964_st2 hsa-asg- hsa-asg- 7.22 7.48 7.64 7.45 0.21 100 6.89 6.98 5.065.95 6.49 7.01 4.94 6.34 4.57 6.03 0.95 67 3.16E−02 1.42 2.7 62776277_st2 hsa-miR- hsa-miR- 7.41 7.69 7.42 7.50 0.16 100 6.42 6.72 6.066.22 5.80 6.66 5.59 5.77 5.53 6.09 0.45 100 3.85E−04 1.42 2.7 142-5p142-5p_st2 hsa-asg- hsa-asg- 5.80 5.97 6.11 5.96 0.15 67 4.81 4.61 4.215.39 5.05 4.46 4.49 3.20 4.67 4.54 0.61 0 3.18E−03 1.41 2.7 1246512465_st1 hsa-asg- hsa-asg- 3.73 5.56 4.00 4.43 0.99 33 4.31 3.42 3.063.73 3.44 2.69 1.60 2.78 2.33 3.04 0.80 0 3.25E−02 1.39 2.6 39763976_st1 hsa-asg- hsa-asg- 5.54 4.91 5.73 5.40 0.43 33 4.02 4.30 3.064.12 4.60 4.37 3.68 4.14 3.77 4.01 0.46 0 9.60E−04 1.39 2.6 1251512515_st2 hsa-asg- hsa-asg- 6.36 6.50 6.47 6.44 0.07 100 5.48 5.06 4.966.07 5.36 4.52 5.05 3.78 5.20 5.05 0.64 11 4.42E−03 1.39 2.6 57155715_st2 hsa-asg- hsa-asg- 7.19 6.14 7.21 6.85 0.61 100 5.68 5.19 5.286.40 6.25 5.59 4.98 4.47 5.35 5.46 0.60 33 6.46E−03 1.38 2.6 31453145_st1 hsa-miR- hsa-miR- 13.2 13.2 13.25 13.2 0.02 100 12.2 12.1 11.912.5 12.1 11.7 11.5 10.8 11.8 11.8 0.48 100 6.67E−04 1.38 2.6 30a-5p30a-5p_st2 hsa-miR-93 hsa-miR- 12.1 12.2 12.33 12.2 0.11 100 10.6 10.811.0 11.5 10.9 10.4 10.9 10.3 11.0 10.8 0.36 100 8.27E−05 1.37 2.693_st2 hsa-asg- hsa-asg- 5.54 6.21 5.22 5.66 0.50 100 5.29 4.76 4.545.04 4.42 4.18 3.29 3.91 3.25 4.30 0.72 22 1.31E−02 1.36 2.6 259 259_st2hsa-asg-12277 hsa-asg- 5.03 5.08 5.38 5.16 0.19 67 3.85 3.64 4.02 4.713.72 3.26 3.68 3.44 3.97 3.81 0.42 0 3.38E−04 1.35 2.6 12277_st1hsa-miR- hsa-miR- 12.3 12.1 12.22 12.2 0.11 100 11.2 11.1 11.2 11.4 11.410.8 10.4 10.1 10.0 10.9 0.55 100 2.27E−03 1.35 2.5 320 320_st1hsa-miR-98 hsa-miR- 12.4 12.2 12.39 12.3 0.08 100 11.5 11.0 11.1 11.511.0 10.8 10.9 10.1 10.6 11.0 0.43 100 4.25E−04 1.34 2.5 98_st1 hsa-miR-hsa-miR- 10.1 10.6 10.92 10.5 0.39 100 10.0 9.44 9.16 9.78 9.72 7.508.94 8.77 9.53 9.21 0.76 100 1.70E−02 1.34 2.5 29c 29c_st2 hsa-asg-hsa-asg- 7.93 8.08 8.05 8.02 0.08 100 7.33 7.11 5.45 7.16 6.78 6.86 6.786.84 5.84 6.68 0.63 33 5.10E−03 1.34 2.5 872 872_st2 hsa-asg-10674hsa-asg- 6.22 6.07 6.26 6.18 0.10 100 4.31 4.49 5.24 5.42 5.21 5.30 4.734.35 4.67 4.86 0.44 0 4.97E−04 1.33 2.5 10674_st1 hsa-asg- hsa-asg- 6.776.85 7.07 6.90 0.16 100 5.68 5.25 6.12 6.30 5.59 5.60 5.35 5.06 5.205.57 0.42 11 4.03E−04 1.32 2.5 11181 11181_st1 hsa-asg- hsa-asg- 5.205.75 5.66 5.54 0.30 33 4.31 4.43 4.30 4.69 4.65 3.59 4.33 3.32 4.40 4.220.46 0 1.08E−03 1.31 2.5 7351 7351_st2 hsa-miR-96 hsa-miR- 4.46 3.955.35 4.59 0.71 33 4.99 3.42 3.06 3.01 4.11 2.85 3.05 1.88 3.09 3.27 0.8611 4.00E−02 1.31 2.5 96_st1 hsa-cand283 hsa-cand283_st1 6.57 6.46 6.416.48 0.08 100 5.45 6.14 4.68 5.56 4.74 5.63 5.25 4.58 4.54 5.17 0.57 333.11E−03 1.31 2.5 hsa-asg- hsa-asg- 5.81 5.49 5.24 5.51 0.29 100 5.324.16 4.12 4.49 4.00 3.87 4.14 3.44 4.32 4.21 0.51 11 2.07E−03 1.31 2.513905 13905_st1 hsa-asg-13471 hsa-asg- 5.66 5.87 6.26 5.93 0.30 100 4.764.76 4.39 5.12 5.35 5.40 4.38 4.07 3.53 4.64 0.61 11 6.58E−03 1.29 2.413471_st1 hsa-asg- hsa-asg-6543_st1 5.92 4.96 4.69 5.19 0.65 33 4.243.64 4.12 4.26 4.33 3.95 3.50 3.74 3.39 3.91 0.35 0 1.15E−03 1.28 2.46543 hsa-asg- hsa-asg- 7.36 7.18 7.14 7.23 0.12 100 6.73 6.14 5.98 7.056.17 5.01 6.26 4.87 5.31 5.95 0.75 67 1.68E−02 1.28 2.4 4448 4448_st1hsa-miR- hsa-miR- 13.7 13.5 13.61 13.6 0.08 100 12.7 12.1 12.4 12.9 12.411.8 12.1 11.6 12.5 12.3 0.43 100 5.88E−04 1.27 2.4 30d 30d_st1 hsa-asg-hsa-asg- 13.0 12.7 12.79 12.8 0.20 100 12.2 11.9 11.2 12.2 12.0 11.411.3 10.4 11.4 11.6 0.58 100 4.88E−03 1.27 2.4 11199 11199_st2 hsa-asg-hsa-asg- 5.18 4.49 4.76 4.81 0.35 33 4.02 3.29 4.39 4.23 3.77 3.13 2.642.93 3.53 3.55 0.60 0 6.86E−03 1.26 2.4 1221_st2 1221_st2 hsa-miR-hsa-miR- 11.7 11.9 11.77 11.8 0.09 100 10.5 10.7 10.5 11.5 11.5 9.8910.2 9.40 10.6 10.5 0.70 100 1.27E−02 1.26 2.4 199a 199a_st2 hsa-miR-hsa-miR- 13.1 13.0 13.09 13.1 0.05 100 12.2 11.8 11.9 12.6 12.2 11.411.7 10.9 11.7 11.8 0.50 100 1.92E−03 1.24 2.4 99a 99a_st2 hsa-asg-hsa-asg- 6.40 6.21 6.54 6.38 0.17 100 6.02 4.95 4.75 6.06 5.19 4.63 4.905.26 4.54 5.14 0.56 11 4.31E−03 1.24 2.4 510 510_st1 hsa-asg-10816hsa-asg- 4.29 5.42 4.96 4.89 0.57 33 4.24 4.37 4.02 4.66 3.59 2.17 4.142.78 2.92 3.65 0.85 0 4.28E−02 1.24 2.4 10816_st2 hsa-miR- hsa-miR- 12.512.1 12.33 12.3 0.18 100 12.0 11.0 11.2 11.7 11.3 10.5 11.2 9.98 10.911.1 0.61 100 7.22E−03 1.23 2.3 10a 10a_st1 hsa-asg- hsa-asg- 6.33 6.105.56 6.00 0.39 100 4.94 4.30 5.19 5.19 4.89 4.65 4.27 4.47 5.05 4.770.36 100 5.55E−04 1.23 2.3 12471 12471_st1 hsa-asg- hsa-asg- 5.35 5.595.38 5.44 0.13 33 3.45 5.19 3.39 4.63 3.10 5.11 4.20 4.14 4.78 4.22 0.770 2.53E−02 1.21 2.3 3821 3821_st2 hsa-miR- hsa-miR- 13.4 13.4 13.37 13.40.05 100 13.1 12.2 12.2 13.1 12.6 11.8 11.7 11.1 11.9 12.2 0.65 1001.12E−02 1.21 2.3 100 100_st2 hsa-asg- hsa-asg-2967_st2 5.80 5.49 6.425.90 0.47 100 5.18 4.86 4.54 4.81 5.95 4.43 3.93 4.35 4.32 4.71 0.59 221.04E−02 1.20 2.3 2967 hsa-miR- hsa-miR- 10.1 10.0 10.21 10.1 0.10 1008.04 9.33 9.47 9.54 8.91 9.18 8.03 8.82 9.17 8.94 0.57 100 5.49E−03 1.202.3 34a 34a_st1 hsa-miR-22 hsa-miR- 12.6 12.7 12.71 12.7 0.04 100 11.711.6 12.0 12.0 11.6 11.0 11.1 11.2 11.1 11.5 0.38 100 3.62E−04 1.19 2.322_st1 hsa-asg- hsa-asg- 6.41 6.01 5.81 6.08 0.31 67 4.37 5.71 5.21 4.495.36 5.03 3.85 4.89 5.07 4.89 0.57 0 6.67E−03 1.19 2.3 11877 11877_st1hsa-asg- hsa-asg- 7.46 6.85 6.77 7.03 0.38 100 6.30 5.77 6.23 6.49 5.954.84 5.80 4.68 6.48 5.84 0.67 78 1.66E−02 1.19 2.3 14146 14146_st1hsa-asg- hsa-asg- 5.10 4.84 5.36 5.10 0.26 33 4.37 4.00 3.67 3.91 3.934.49 3.40 3.64 3.87 3.92 0.35 0 3.21E−04 1.18 2.3 10830 10830_st2 hsa-hsa- 4.94 5.20 6.02 5.39 0.56 67 4.76 5.04 3.54 4.71 5.15 4.18 2.64 3.993.97 4.22 0.80 11 4.40E−02 1.17 2.2 cand330 cand330_st2 hsa-asg-hsa-asg- 4.52 4.62 4.90 4.68 0.20 33 4.17 3.02 3.23 4.55 4.11 4.03 2.483.32 2.74 3.52 0.72 0 2.29E−02 1.16 2.2 4158 4158_st1 hsa-asg- hsa-asg-5.45 5.46 4.64 5.18 0.47 67 5.03 3.42 3.67 4.49 4.63 4.18 3.17 3.54 4.164.03 0.62 11 1.54E−02 1.15 2.2 6803 6803_st1 hsa-miR- hsa-miR- 12.6 12.712.71 12.6 0.06 100 12.1 11.4 12.0 12.0 11.5 10.8 11.4 11.0 11.1 11.50.47 100 2.15E−03 1.15 2.2 106a 106a_st1 hsa-miR- hsa-miR- 6.53 6.436.82 6.59 0.20 100 4.76 5.12 6.41 6.24 5.05 5.24 4.73 5.11 6.41 5.450.70 56 2.14E−02 1.14 2.2 34b 34b_st2 hsa-miR- hsa-miR- 10.3 10.3 10.1210.2 0.14 100 10.2 8.61 9.99 9.95 9.07 8.06 9.21 8.52 8.59 9.13 0.76 1003.20E−02 1.14 2.2 197 197_st1 hsa-miR- hsa-miR- 12.1 12.4 12.40 12.30.15 100 11.6 11.6 11.2 11.9 11.5 11.6 11.1 10.1 10.0 11.2 0.68 1001.86E−02 1.14 2.2 30c 30c_st1 hsa- hsa- 9.77 9.49 9.26 9.51 0.25 1008.90 8.31 8.71 8.69 8.14 7.82 7.95 8.41 8.42 8.37 0.36 100 5.48E−04 1.142.2 miR102 miR102_st2 hsa-asg- hsa-asg- 6.91 7.39 7.27 7.19 0.25 0 5.606.42 6.43 6.33 5.78 6.22 6.76 5.42 5.63 6.06 0.47 11 2.93E−03 1.13 2.28476 8476_st1 hsa-asg- hsa-asg- 6.23 6.53 6.34 6.37 0.15 100 5.62 4.765.71 5.53 5.68 5.20 4.78 5.19 4.73 5.24 0.41 22 1.12E−03 1.12 2.2 58275827_st2 hsa-miR- hsa-miR- 5.42 5.79 6.18 5.79 0.38 67 5.11 4.76 5.044.84 4.72 4.28 4.59 4.07 4.67 4.67 0.33 0 6.23E−04 1.12 2.2 624 624_st2hsa-asg- hsa-asg-3902_st1 4.97 5.56 5.77 5.43 0.41 67 4.94 4.55 4.305.19 4.45 4.03 4.14 3.20 4.07 4.32 0.58 0 1.24E−02 1.11 2.2 3902hsa-asg- hsa-asg- 6.72 6.46 6.38 6.52 0.18 100 5.68 4.81 5.71 5.77 5.545.01 5.12 5.29 5.78 5.41 0.36 22 5.64E−04 1.11 2.2 3798 3798_st1hsa-miR- hsa-miR- 9.89 9.77 10.50 10.0 0.39 100 9.83 9.37 8.91 9.35 8.958.34 8.55 8.21 9.05 8.95 0.52 100 7.81E−03 1.11 2.2 194 194_st2 hsa-asg-hsa-asg- 6.02 5.82 6.63 6.16 0.43 67 5.65 5.51 3.31 4.93 5.49 5.24 4.785.31 5.29 5.05 0.71 0 3.16E−02 1.10 2.1 13661 13661_st1 hsa-asg-hsa-asg-11883_st1 7.05 7.27 7.21 7.18 0.12 100 5.92 6.06 6.67 6.74 6.186.02 6.12 4.58 6.44 6.08 0.63 78 1.60E−02 1.10 2.1 11883 hsa-miR-hsa-miR- 13.0 13.0 13.04 13.0 0.01 100 12.4 11.9 12.5 12.3 11.8 11.211.9 11.4 11.6 11.9 0.44 100 1.97E−03 1.10 2.1 17-5p 17-5p_st1 hsa-asg-hsa-asg- 7.12 6.21 7.43 6.92 0.63 100 6.80 6.02 5.45 6.11 5.42 5.17 5.515.79 6.12 5.82 0.50 56 1.11E−02 1.10 2.1 6138 6138_st1 hsa-miR- hsa-miR-13.7 13.7 13.59 13.6 0.09 100 13.1 12.9 12.4 13.1 12.8 12.3 12.4 11.812.2 12.6 0.43 100 1.79E−03 1.09 2.1 143 143_st2 hsa- hsa- 5.08 4.895.49 5.15 0.31 33 4.55 4.49 3.91 4.15 3.44 4.24 2.92 3.83 5.05 4.06 0.6311 1.88E−02 1.09 2.1 cand190 cand190_st1 hsa-asg- hsa-asg- 6.91 6.066.43 6.46 0.43 100 5.87 6.49 4.17 5.04 4.99 6.07 5.09 5.06 5.64 5.380.70 0 3.28E−02 1.08 2.1 7118 7118_st1 hsa-miR- hsa-miR- 7.60 7.88 7.617.70 0.16 100 6.51 5.65 6.84 7.08 7.11 6.71 6.11 6.48 7.02 6.61 0.49 894.24E−03 1.08 2.1 210 210_st1 hsa-asg- hsa-asg-12765_st2 6.71 6.58 6.306.53 0.21 100 5.75 5.89 5.45 6.07 5.46 5.20 5.46 4.96 4.78 5.45 0.42 331.85E−03 1.08 2.1 12765 hsa-miR- hsa-miR- 12.7 12.4 12.51 12.5 0.12 10011.7 11.6 11.2 12.0 11.7 11.7 11.1 10.9 11.1 11.4 0.37 100 7.33E−04 1.082.1 361 361_st2 hsa-asg- hsa-asg- 5.16 5.32 4.82 5.10 0.25 67 4.66 3.744.47 4.71 5.19 2.69 3.40 3.74 3.65 4.03 0.78 0 4.65E−02 1.07 2.1 1051310513_st1 hsa-asg- hsa-asg- 4.90 5.03 5.02 4.98 0.07 67 3.93 3.83 3.794.69 4.30 3.78 3.85 3.49 3.65 3.92 0.36 0 6.26E−04 1.06 2.1 30553055_st2 hsa-asg- hsa-asg- 5.45 5.52 5.43 5.47 0.05 100 4.85 4.30 4.874.30 4.89 3.48 4.69 4.07 4.24 4.41 0.47 0 3.55E−03 1.06 2.1 56955695_st2 hsa-miR- hsa-miR- 11.8 12.1 12.11 12.0 0.16 100 11.6 1.2 1.111.5 11.1 10.9 10.9 10.0 9.96 10.9 0.58 100 1.35E−02 1.05 2.1 30b30b_st2 hsa-miR- hsa-miR- 13.3 13.4 13.33 13.4 0.08 100 12.6 12.2 12.112.8 12.3 11.9 12.1 12.1 12.3 12.3 0.30 100 1.77E−04 1.04 2.1 221221_st1 hsa-asg-14048 hsa-asg- 5.29 4.79 5.93 5.34 0.57 67 4.43 4.953.54 3.63 5.03 3.26 4.59 5.15 4.07 4.29 0.70 0 4.42E−02 1.04 2.114048_st1 hsa-asg- hsa-asg- 5.18 4.94 5.55 5.22 0.31 33 3.56 3.47 4.434.58 4.11 3.37 4.00 5.58 4.57 4.18 0.70 0 3.50E−02 1.04 2.1 44414441_st1 hsa-asg- hsa-asg-10081_st1 5.82 5.97 5.51 5.77 0.23 100 5.754.30 4.47 5.34 5.12 3.95 4.94 4.47 4.24 4.73 0.59 11 1.60E−02 1.04 2.110081 hsa-asg- hsa-asg- 7.78 7.52 7.98 7.76 0.23 67 6.79 6.98 7.19 7.296.98 6.52 6.22 6.32 6.27 6.73 0.41 22 2.26E−03 1.03 2.0 12346 12346_st2hsa-asg- hsa-asg- 6.71 6.51 6.29 6.50 0.21 100 5.57 5.74 5.71 5.86 5.244.97 5.12 5.62 5.42 5.47 0.30 33 3.12E−04 1.03 2.0 2365 2365_st1hsa-asg- hsa-asg- 3.12 2.81 4.21 3.38 0.73 33 3.21 2.87 2.18 2.85 2.012.17 1.08 1.88 2.92 2.35 0.67 0 4.80E−02 1.03 2.0 1947 1947_st1hsa-let-7e hsa-let- 13.4 13.2 13.21 13.3 0.15 100 12.6 12.8 12.3 12.612.3 12.6 12.0 11.3 11.8 12.2 0.48 100 5.63E−03 1.02 2.0 7e_st1 hsa-asg-hsa-asg- 6.59 6.55 6.52 6.55 0.04 100 5.68 5.72 5.28 6.14 6.02 4.75 6.194.87 5.23 5.54 0.54 56 1.00E−02 1.01 2.0 13086 13086_st1 hsa-asg-hsa-asg- 4.17 5.03 5.35 4.85 0.61 33 3.93 4.08 4.21 4.08 4.11 3.48 4.003.74 2.92 3.84 0.41 0 7.66E−03 1.01 2.0 14008 14008_st2 hsa-asg-hsa-asg- 6.45 6.35 6.18 6.33 0.14 100 6.16 5.04 5.45 5.68 5.39 4.93 4.825.04 5.35 5.32 0.42 11 2.70E−03 1.01 2.0 12179 12179_st1 hsa-asg-hsa-asg- 8.02 7.39 8.42 7.94 0.52 100 7.52 7.52 6.10 6.92 7.19 6.82 6.606.86 6.89 6.94 0.44 89 8.30E−03 1.01 2.0 3340 3340_st2 hsa-asg-hsa-asg-13350_st2 9.73 9.16 10.01 9.63 0.44 100 9.29 9.08 8.31 8.84 8.618.88 8.30 8.27 8.07 8.63 0.42 44 5.00E−03 1.01 2.0 13350 hsa-asg-hsa-asg- 6.60 7.19 6.85 6.88 0.30 100 5.87 6.55 4.78 6.21 6.10 6.92 4.905.92 5.64 5.88 0.70 33 4.05E−02 1.00 2.0 9053 9053_st1 SD, standarddeviation; %, percentage of samples expressing miRNA above backgroundlevels; Log2Diff, difference in VSN-transformed expression between NBLand LNneg samples.

Example 21 miRNAs Differentally Expressed Between Normal EsophagusTissue and Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous esophagus cells to lymph nodes, the inventors identifiedmiRNAs that are expressed at significantly higher levels in normalesophagus tissue than in cancer-negative lymph nodes.

Total RNA, purified from three separate normal human esophagus tissuesamples (FirstChoice® Human Esophagus Total RNA, cat. no. AM6842;Ambion, Austin, Tex., USA) and from nine normal human lymph node samples(FirstChoice® Human Lymph Node Total RNA, cat. no. AM7894; Ambion,Austin, Tex., USA) was purchased from Ambion. miRNA purification andlabeling and microarray expression analyses were performed as describedabove in Example 2.

The inventors identified 238 miRNAs (93 verified, 145 predicted) withsignificant differential expression between normal esophagus tissuesamples and normal lymph nodes (log 2Diff esophagus vs LN neg≧1 andp-value<0.05) (Table 20). Among the verified miRNAs, hsa-miR-205, -206and -187 had average expression levels that were more than 100-foldhigher in esophagus samples than in normal lymph node samples;hsa-miR-203, -133b, and -429 had average expression levels that weremore than 50-fold higher in esophagus samples, 10 additional miRNAs hadaverage expression levels that were between 10- and 50-fold higher inesophagus samples; and 22 other miRNAs had average expression levelsthat were between 5- and 10-fold higher in esophagus samples. Among thepredicted miRNAs, hsa-asg-8428_st2 and -14153_st2 had average expressionlevels that were over 20-fold higher in esophagus samples than in normallymph nodes. Seven miRNAs had average expression levels that werebetween 10- and 20-fold higher in esophagus compared to lymph nodesamples.

TABLE 20 MicroRNAs Differentially Expressed Between Normal EsophagusSamples (ESO) and Cancer-Negative Lymph Node Samples (LNneg) fromPatients. ESO LNneg MicroRNA Probe ID 1 2 3 M SD % 1 2 3 4 5 6 7 8 9 MSD % ttest log2Diff Fold hsa-miR-205 hsa-miR-205_st1 13.3 13.4 13.2 13.30.08 100 5.64 2.03 3.59 6.11 3.40 5.32 7.76 2.93 5.91 2.56 0.80 01.45E−05 8.61 389 hsa-miR-206 hsa-miR-206_st2 12.4 13.0 12.7 12.7 0.33100 1.92 9.39 3.97 5.85 8.46 4.47 5.00 4.09 4.99 2.40 0.96 0 3.14E−047.40 169 hsa-miR-187 hsa-miR-187_st1 10.1 9.97 10.8 10.3 0.46 100 2.763.77 3.22 3.37 3.05 3.38 3.17 4.76 2.79 4.25 0.73 22 6.23E−09 6.95 123hsa-miR-203 hsa-miR-203_st2 13.4 13.4 13.4 13.4 0.02 100 7.21 8.24 6.455.41 5.19 6.07 9.36 7.33 5.89 6.13 0.74 56 1.07E−05 6.62 98.3hsa-miR-133b hsa-miR-133b_st2 12.1 12.6 12.5 12.4 0.27 100 4.47 10.17.45 6.20 7.22 4.47 5.39 7.36 6.22 4.24 0.31 0 2.53E−04 5.90 59.5hsa-miR-429 hsa-miR-429_st1 8.64 8.37 8.70 8.57 0.18 100 3.67 2.13 2.372.69 2.32 2.45 5.39 3.45 1.54 6.66 0.51 78 8.14E−06 5.68 51.3hsa-miR-133a hsa-miR-133a_st1 12.8 13.0 13.0 12.9 0.15 100 5.92 10.88.19 7.14 8.22 6.25 6.48 7.77 7.70 4.89 0.62 44 1.12E−04 5.36 41.0hsa-miR-375 hsa-miR-375_st1 10.1 10.6 7.21 9.34 1.86 67 6.23 2.85 2.973.69 0.99 4.63 6.39 5.96 4.93 5.36 0.70 44 2.01E−03 5.05 33.1hsa-miR-200b hsa-miR-200b_st1 12.6 12.6 12.6 12.6 0.03 100 8.90 6.696.82 6.87 6.51 7.65 10.4 7.62 7.80 5.77 0.34 78 6.41E−05 4.94 30.7hsa-miR-499 hsa-miR12_st1 9.45 9.72 9.72 9.63 0.16 100 5.37 6.66 4.114.43 4.82 4.58 3.87 5.45 4.81 6.05 0.32 100 2.79E−06 4.73 26.5hsa-miR-499 hsa-miR-499_st1 9.20 9.47 9.54 9.40 0.18 100 4.75 6.58 4.534.18 5.05 4.28 4.60 5.32 4.66 3.29 0.98 0 1.17E−06 4.52 23.0hsa-asg-8428 hsa-asg-8428_st2 6.24 6.50 6.54 6.43 0.16 100 2.66 2.032.53 1.01 0.02 2.17 1.62 2.25 1.96 5.18 0.55 11 3.12E−06 4.62 24.6hsa-asg-14153 hsa-asg-14153_st2 7.00 7.60 7.04 7.21 0.34 33 3.06 3.012.37 2.69 1.16 2.78 4.40 3.07 2.26 4.36 0.51 11 6.54E−06 4.46 22.0hsa-miR-200c hsa-miR-200c_st1 13.3 13.5 13.5 13.4 0.11 100 10.3 8.188.15 8.14 7.98 8.89 11.5 9.60 8.84 5.10 0.41 78 1.21E−04 4.37 20.7hsa-asg-9198 hsa-asg-9198_st1 5.24 6.28 6.20 5.91 0.58 100 1.39 1.112.11 1.78 1.60 1.11 1.86 2.05 1.54 5.62 0.29 33 2.76E−08 4.29 19.6hsa-miR-200a hsa-miR-200a_st2 10.0 9.82 9.46 9.77 0.29 100 6.49 5.725.29 4.60 4.07 4.78 7.78 6.27 5.08 7.47 0.36 100 1.08E−04 4.21 18.5hsa-miR-576 hsa-miR-576_st2 6.69 6.23 6.57 6.50 0.24 100 3.06 1.72 2.011.97 1.97 2.08 3.09 2.25 2.87 6.92 0.63 100 1.42E−07 4.16 17.9hsa-asg-1794 hsa-asg-1794_st1 5.88 5.95 5.96 5.93 0.04 100 2.66 1.722.19 1.01 2.15 1.48 2.92 1.85 1.96 3.62 0.65 56 4.81E−07 3.93 15.3hsa-asg-924 hsa-asg-924_st1 7.79 7.42 6.57 7.26 0.62 100 3.39 4.46 2.692.77 2.15 2.78 3.53 4.66 4.30 4.14 0.53 0 4.64E−05 3.85 14.4 hsa-miR-605hsa-miR-605_st1 6.10 6.36 6.51 6.32 0.21 100 3.06 0.75 1.74 2.17 2.923.07 4.47 2.61 2.16 6.92 0.78 89 1.17E−04 3.77 13.7 hsa-asg-2027hsa-asg-2027_st1 7.53 7.46 7.83 7.60 0.20 100 1.66 5.23 4.47 4.24 4.274.21 4.54 3.56 3.53 3.36 1.04 0 1.26E−04 3.64 12.4 hsa-asg-5602hsa-asg-5602_st2 7.58 6.95 7.66 7.40 0.39 33 3.79 6.15 4.68 3.37 3.731.48 3.77 3.56 3.47 6.02 0.53 89 6.21E−04 3.62 12.3 hsa-asg-2345hsa-asg-2345_st2 6.15 5.88 5.59 5.88 0.28 100 2.43 3.63 1.46 2.53 3.172.62 1.38 1.65 2.54 5.60 0.58 33 1.95E−05 3.50 11.3 hsa-miR-338hsa-miR-338_st1 8.01 7.59 7.82 7.81 0.21 100 5.57 4.86 4.04 2.17 4.783.44 4.89 5.11 4.08 5.53 0.75 33 2.22E−04 3.48 11.2 hsa-asg-7637hsa-asg-7637_st2 6.72 7.75 7.20 7.22 0.51 100 4.47 3.46 3.93 4.03 3.293.64 5.27 3.66 2.54 4.93 0.65 56 3.29E−05 3.41 10.7 hsa-miR-189hsa-miR-189_st1 8.97 8.29 9.02 8.76 0.41 100 4.93 7.23 6.18 4.55 4.943.54 4.95 6.08 5.79 7.94 0.77 100 4.12E−04 3.41 10.6 hsa-asg-11715hsa-asg-11715_st2 5.34 5.84 4.46 5.21 0.70 67 2.18 1.51 2.11 1.78 0.621.48 3.39 1.85 2.36 3.81 0.89 0 5.77E−05 3.29 9.8 hsa-miR-378hsa-miR-378_st1 7.29 7.56 7.76 7.53 0.24 100 4.84 6.39 5.46 2.17 6.163.59 0.63 4.71 4.46 8.06 0.77 100 1.54E−02 3.27 9.6 hsa-asg-791hsa-asg-791_st1 5.29 5.11 5.35 5.25 0.13 0 1.66 2.03 2.19 2.44 0.48 1.482.54 2.25 3.02 4.64 0.97 22 2.43E−05 3.24 9.4 hsa-asg-10131hsa-asg-10131_st2 6.44 6.12 6.78 6.45 0.33 100 3.54 2.51 2.53 3.26 2.643.20 5.09 3.33 3.16 8.46 0.92 100 5.45E−05 3.20 9.2 hsa-asg-10393hsa-asg-10393_st1 5.40 6.24 6.02 5.89 0.44 67 2.43 3.91 2.83 2.85 1.793.54 2.54 2.78 1.54 5.57 0.46 22 4.36E−05 3.20 9.2 hsa-asg-hsa-asg-12142_st1 5.43 5.49 5.59 5.50 0.08 100 2.18 3.52 1.83 1.78 2.781.11 2.33 2.61 2.71 11.0 0.65 100 1.78E−05 3.19 9.1 12142 hsa-asg-1826hsa-asg-1826_st2 5.62 6.16 5.86 5.88 0.27 100 2.18 3.57 2.53 2.17 2.923.13 1.86 3.33 3.02 5.94 1.24 67 5.58E−06 3.13 8.8 hsa-asg-hsa-asg-11380_st2 5.74 5.84 5.23 5.60 0.33 100 3.06 2.51 2.19 1.97 2.482.27 3.09 2.93 1.75 4.08 1.08 22 1.12E−06 3.13 8.8 11380 hsa-asg-hsa-asg-13997_st2 5.31 5.82 5.66 5.60 0.26 100 2.18 3.01 2.19 2.35 1.253.20 2.33 3.07 3.29 4.73 0.59 11 1.85E−05 3.05 8.3 13997 hsa-miR-141hsa-miR-141_st1 7.60 7.09 6.96 7.22 0.34 100 4.93 4.43 4.39 2.85 3.853.83 4.54 5.07 3.83 4.65 0.35 0 2.80E−05 3.03 8.1 hsa-miR-149hsa-miR-149_st2 6.04 5.86 6.25 6.05 0.20 33 1.79 4.15 2.76 1.48 2.483.38 3.17 4.49 3.87 4.64 0.77 11 7.00E−04 2.99 7.9 hsa-asg-3910hsa-asg-3910_st2 6.14 5.94 5.75 5.94 0.19 100 2.86 4.33 3.39 2.69 1.791.48 3.65 3.56 2.87 3.64 0.86 0 2.59E−04 2.99 7.9 hsa-miR-561hsa-miR-561_st1 5.77 4.58 5.20 5.18 0.59 33 3.67 2.13 1.64 2.35 2.322.27 2.54 1.25 1.75 7.77 0.51 100 5.46E−05 2.97 7.8 Has-cand26hsa-cand26_st2 7.73 8.12 8.04 7.97 0.21 100 6.03 4.08 3.68 4.75 5.294.07 5.46 6.12 5.61 4.70 0.27 11 2.78E−04 2.96 7.8 hsa-miR-495hsa-miR-495_st1 8.08 8.19 8.36 8.21 0.14 100 6.97 6.26 6.32 4.43 5.162.45 4.89 6.02 4.81 5.49 0.60 33 4.32E−03 2.95 7.8 hsa-miR-31hsa-miR-31_st2 12.6 11.9 11.9 12.1 0.39 100 8.95 10.1 8.71 8.60 7.738.68 10.1 10.4 9.79 3.36 0.96 0 3.53E−04 2.94 7.7 hsa-asg-hsa-asg-13705_st1 5.07 5.25 5.35 5.22 0.14 67 3.79 2.33 2.69 2.35 0.353.20 3.25 1.65 0.95 4.14 0.38 0 1.40E−03 2.94 7.7 13705 hsa-asg-hsa-asg-13135_st1 7.83 8.10 8.25 8.06 0.21 100 5.24 4.91 4.79 4.75 5.164.47 5.60 5.62 5.67 3.47 0.47 0 6.64E−07 2.93 7.6 13135 hsa-asg-9723hsa-asg-9723_st2 4.64 5.49 6.07 5.40 0.72 67 2.86 2.60 1.11 2.17 2.323.69 2.10 2.61 2.87 4.49 0.42 0 1.03E−04 2.92 7.6 hsa-asg-hsa-asg-12753_st1 5.07 5.52 5.02 5.20 0.27 67 2.86 1.51 3.10 2.17 2.642.45 1.86 1.85 2.36 2.41 0.68 0 3.79E−06 2.89 7.4 12753 hsa-asg-hsa-asg-10247_st1 5.03 5.63 5.49 5.38 0.31 33 4.02 1.72 2.19 2.35 0.353.20 3.97 2.78 2.16 7.42 0.88 100 2.00E−03 2.85 7.2 10247 hsa-miR-493-hsa-miR-493- 7.40 7.33 7.86 7.53 0.29 100 6.88 6.18 6.33 3.69 4.38 1.884.60 4.37 3.83 3.83 0.53 0 1.24E−02 2.85 7.2 5p 5p_st1 hsa-asg-6178hsa-asg-6178_st2 4.73 4.99 5.71 5.14 0.51 33 2.43 3.82 2.19 1.19 0.762.27 2.33 2.43 3.42 5.26 1.04 22 6.87E−04 2.83 7.1 hsa-asg-1765hsa-asg-1765_st1 5.43 5.86 4.95 5.41 0.46 67 2.86 3.63 2.37 2.35 3.292.27 2.10 2.25 2.16 3.11 1.22 0 1.16E−05 2.83 7.1 hsa-asg-1587hsa-asg-1587_st1 5.37 5.31 5.13 5.27 0.13 67 3.06 1.51 3.10 2.35 2.782.45 2.54 2.25 1.96 4.73 0.52 0 3.33E−06 2.83 7.1 hsa-asg-8054hsa-asg-8054_st1 5.46 4.95 4.65 5.02 0.41 33 1.39 1.72 2.37 1.19 3.052.08 2.54 3.45 2.36 5.39 0.42 44 1.15E−04 2.78 6.9 hsa-asg-5617hsa-asg-5617_st1 5.67 5.46 5.57 5.57 0.10 100 3.91 3.63 3.44 2.69 1.421.48 1.62 3.76 3.29 6.24 0.52 44 1.17E−03 2.76 6.8 hsa-miR-411hsa-miR-411_st1 6.77 6.01 6.99 6.59 0.52 100 4.54 5.23 5.56 2.99 3.402.27 2.10 4.85 3.74 5.14 0.48 22 5.26E−03 2.74 6.7 hsa-asg-3822hsa-asg-3822_st2 5.73 5.33 5.29 5.45 0.25 33 3.67 2.03 2.45 2.17 1.602.93 4.78 2.05 3.02 6.03 0.41 100 1.06E−03 2.70 6.5 hsa-miR-hsa-miR-200a-_st2 5.72 5.28 5.26 5.42 0.26 67 2.18 3.91 2.61 1.78 2.153.20 2.92 3.07 2.87 3.74 0.91 0 4.77E−05 2.68 6.4 200a* hsa-asg-2399hsa-asg-2399_st2 5.28 4.58 5.66 5.17 0.55 33 2.86 3.01 2.19 2.53 1.082.27 2.54 3.07 3.42 4.74 0.50 0 1.33E−04 2.62 6.2 hsa-asg-2484hsa-asg-2484_st2 5.57 4.87 5.23 5.22 0.35 67 1.39 4.00 2.19 2.35 3.402.78 2.54 2.43 2.36 4.10 0.28 0 1.81E−04 2.62 6.1 hsa-asg-hsa-asg-13908_st2 5.43 4.82 5.49 5.25 0.37 67 3.67 2.51 1.46 2.85 2.642.45 3.77 2.05 2.54 7.74 0.31 100 1.66E−04 2.59 6.0 13908 hsa-asg-5021hsa-asg-5021_st1 7.12 7.24 7.36 7.24 0.12 100 6.99 6.06 5.91 3.54 4.593.07 2.10 5.18 4.43 2.75 0.63 0 2.00E−02 2.59 6.0 hsa-miR-368hsa-miR-368_st2 8.88 8.72 8.95 8.85 0.12 100 7.70 8.08 7.70 5.24 5.643.99 5.09 6.49 6.52 7.73 0.74 100 1.12E−02 2.58 6.0 hsa-asg-hsa-asg-10881_st1 7.57 7.79 8.01 7.79 0.22 100 6.06 5.43 4.79 5.46 4.205.04 5.23 5.73 5.04 11.6 0.47 100 1.47E−05 2.57 5.9 10881 hsa-miR-1hsa-miR-1_st1 12.5 12.6 12.4 12.5 0.06 100 8.18 11.7 10.7 10.0 10.1 7.669.47 11.0 10.8 3.70 0.32 0 9.87E−03 2.56 5.9 hsa-miR-381 hsa-miR-381_st25.67 5.34 5.98 5.66 0.32 100 4.21 3.40 4.21 2.99 3.50 1.29 3.25 2.782.36 8.62 0.59 100 9.53E−04 2.55 5.9 hsa-miR-183 hsa-miR-183_st1 9.299.51 9.82 9.54 0.27 100 6.71 7.93 7.56 5.83 7.52 6.32 6.65 7.66 6.766.16 0.72 89 1.41E−04 2.55 5.8 hsa-asg- hsa-asg-12139_st2 5.70 4.48 4.464.88 0.71 33 2.66 2.33 1.64 2.17 1.60 2.08 3.53 2.78 2.36 10.2 0.61 1001.12E−04 2.53 5.8 12139 hsa-miR-582 hsa-miR-582_st1 6.99 6.61 7.51 7.040.45 100 4.54 5.96 4.89 4.10 4.07 4.35 4.67 5.85 2.16 6.38 0.40 783.97E−03 2.53 5.8 hsa-miR-487b hsa-miR-487b_st2 7.79 8.30 8.25 8.11 0.28100 6.97 6.90 6.25 5.40 4.90 3.74 4.40 6.06 5.84 5.36 0.87 0 3.56E−032.51 5.7 hsa-asg-3976 hsa-asg-3976_st1 5.72 5.72 5.16 5.54 0.32 100 3.063.73 3.44 2.69 1.60 2.78 2.33 4.31 3.42 5.23 0.50 11 4.47E−04 2.50 5.6hsa-miR-96 hsa-miR-96_st1 5.90 5.34 6.05 5.76 0.37 100 3.06 3.01 4.112.85 3.05 1.88 3.09 4.99 3.42 5.88 0.69 67 8.05E−04 2.49 5.6 hsa-asg-378hsa-asg-378_st1 4.64 5.65 5.26 5.18 0.51 67 2.18 4.71 2.83 2.99 1.082.78 3.09 2.25 2.71 7.30 0.76 89 2.08E−03 2.45 5.4 hsa-asg-hsa-asg-10080_st1 5.32 4.78 4.23 4.78 0.55 0 2.43 1.82 2.37 2.44 2.482.27 3.39 2.05 1.75 5.29 0.88 11 2.33E−05 2.44 5.4 10080 hsa-miR-9hsa-miR-9_st2 6.40 5.25 5.64 5.76 0.58 100 2.86 3.28 3.63 2.17 3.50 3.324.16 3.45 3.53 9.36 0.86 89 6.41E−05 2.44 5.4 hsa-miR-9 hsa-cand90_st26.20 3.49 4.35 4.68 1.39 33 2.66 3.01 2.83 2.17 2.64 1.68 3.65 3.56 3.167.10 0.76 89 7.52E−03 1.86 3.6 hsa-asg- hsa-asg-11088_st1 4.36 5.05 4.814.74 0.35 0 3.85 1.82 0.94 2.35 1.60 3.38 4.50 1.25 1.05 10.6 0.32 1001.13E−02 2.44 5.4 11088 hsa-asg-1296 hsa-asg-1296_st1 5.76 5.37 5.885.67 0.26 67 4.81 2.42 2.53 2.77 3.17 3.91 4.16 3.07 2.54 3.99 0.66 118.01E−04 2.40 5.3 hsa-asg- hsa-asg-13070_st1 4.99 5.18 4.75 4.97 0.22 332.66 4.15 3.10 2.17 2.32 1.88 1.38 2.93 2.54 4.07 1.08 11 5.17E−04 2.405.3 13070 Has-cand852 hsa-cand852_st2 5.43 4.53 5.35 5.10 0.50 67 2.863.01 2.28 2.69 2.92 2.08 3.53 3.07 1.96 8.29 0.61 100 3.56E−05 2.39 5.2hsa-miR-376a hsa-miR-376a_st2 6.93 6.57 6.78 6.76 0.18 100 5.49 5.674.85 4.03 2.48 3.83 3.77 5.14 4.08 8.41 1.11 100 2.75E−03 2.39 5.2hsa-miR-376a hsa-asg-10049_st1 7.35 6.79 7.21 7.12 0.29 100 6.09 5.565.35 4.10 3.85 4.14 4.67 5.03 4.91 4.87 0.68 11 5.15E−04 2.26 4.8hsa-miR-376a hsa-asg-14234_st1 6.30 5.28 5.69 5.76 0.52 100 3.39 4.004.85 3.69 2.92 4.07 1.86 3.33 4.30 6.17 0.44 67 2.59E−03 2.15 4.5hsa-miR-539 hsa-miR-539_st1 4.36 5.21 5.23 4.93 0.49 67 2.66 3.40 2.692.99 1.97 2.62 1.38 2.05 3.16 5.41 0.58 22 1.67E−04 2.39 5.2 hsa-miR-539hsa-miR177_st1 4.80 4.10 4.65 4.52 0.37 0 1.39 2.42 1.83 1.88 3.50 1.682.10 2.61 2.54 8.28 0.35 78 1.58E−04 2.30 4.9 hsa-asg-6139hsa-asg-6139_st2 4.36 5.49 4.23 4.69 0.69 33 1.66 1.51 3.04 2.17 1.792.45 2.54 3.21 2.71 3.47 0.63 0 2.07E−04 2.35 5.1 hsa-asg-4273hsa-asg-4273_st1 3.61 4.10 4.46 4.05 0.43 0 2.18 2.03 1.64 1.97 1.082.36 1.62 0.88 1.54 6.24 0.63 11 2.48E−05 2.35 5.1 hsa-miR-218hsa-miR-218_st2 11.2 10.5 11.5 11.1 0.51 100 8.82 9.68 9.16 8.64 7.767.45 8.75 9.62 9.27 6.93 0.72 78 6.65E−04 2.34 5.1 hsa-asg-hsa-asg-10055_st1 3.95 4.42 4.51 4.29 0.30 0 2.18 1.11 2.01 2.69 1.422.27 2.92 2.05 0.95 8.14 0.57 100 2.08E−04 2.34 5.0 10055 hsa-asg-9980hsa-asg-9980_st1 5.96 6.24 6.51 6.24 0.27 100 4.02 1.72 3.39 2.85 3.855.08 4.67 5.18 4.49 3.24 0.79 0 6.31E−03 2.32 5.0 hsa-miR-299-hsa-miR-299- 7.18 6.96 7.12 7.08 0.11 100 5.80 6.38 5.99 2.99 5.05 2.453.97 5.73 4.49 4.59 0.40 0 1.86E−02 2.32 5.0 5p 5p_st2 hsa-miR-154hsa-miR-154_st2 5.98 5.28 6.19 5.82 0.48 100 4.30 4.36 4.91 3.13 3.171.29 3.97 3.76 2.54 2.49 0.84 0 6.19E−03 2.32 5.0 hsa-asg-8174hsa-asg-8174_st1 5.03 5.79 5.84 5.55 0.45 67 2.86 3.01 2.90 3.26 2.782.78 4.16 3.33 4.08 5.71 0.75 33 5.51E−05 2.31 5.0 hsa-asg-hsa-asg-13254_st1 6.96 7.45 7.20 7.20 0.24 100 6.65 5.89 5.90 4.03 4.732.45 3.39 5.60 5.37 10.8 0.64 100 1.77E−02 2.31 5.0 13254 hsa-asg-7471hsa-asg-7471_st1 8.21 7.71 7.96 7.96 0.25 100 5.67 6.74 5.70 5.30 5.255.00 6.09 5.60 5.72 4.80 0.42 11 2.68E−05 2.29 4.9 hsa-asg-5119hsa-asg-5119_st1 5.10 4.64 4.65 4.80 0.27 0 1.92 1.93 2.01 2.53 2.922.62 3.65 2.43 2.71 5.08 0.45 33 5.44E−05 2.27 4.8 hsa-asg-hsa-asg-13205_st1 8.83 8.10 9.05 8.66 0.50 100 7.09 7.51 6.90 6.04 6.765.59 4.40 7.02 6.39 7.29 0.47 100 3.29E−03 2.25 4.8 13205 hsa-asg-hsa-asg-10391_st2 6.69 6.36 7.13 6.73 0.39 100 2.96 5.39 5.57 4.03 3.592.70 4.81 5.62 5.68 6.90 1.04 89 1.08E−02 2.25 4.7 10391 hsa-asg-8987hsa-asg-8987_st1 6.34 5.42 5.81 5.85 0.46 67 2.76 3.46 3.54 5.01 1.973.87 4.81 2.25 4.84 2.73 0.48 0 8.74E−03 2.24 4.7 hsa-miR-9*hsa-miR-9-_st2 8.17 7.33 7.86 7.79 0.42 100 5.57 6.11 5.41 5.87 4.944.73 5.23 6.20 6.10 6.03 0.57 44 7.36E−05 2.22 4.6 hsa-asg-hsa-asg-11361_st2 5.07 5.55 6.05 5.56 0.49 67 3.39 3.28 3.63 3.37 3.852.62 4.32 2.78 3.53 5.10 0.64 22 9.36E−05 2.14 4.4 11361_st2 hsa-asg-hsa-asg-14150_st1 8.77 8.01 8.92 8.57 0.49 100 7.01 7.73 6.67 5.80 5.795.15 6.39 7.05 6.35 2.22 0.61 0 1.44E−03 2.13 4.4 14150 hsa-miR-199bhsa-miR-199b_st2 11.2 10.9 11.2 11.1 0.19 100 9.04 10.6 10.3 8.25 9.056.55 8.85 9.40 9.08 7.39 0.53 100 1.38E−02 2.12 4.4 hsa-asg-hsa-asg-12690_st1 5.14 4.82 5.35 5.10 0.26 33 2.66 3.40 3.49 2.85 2.922.93 2.10 3.76 2.87 2.31 0.94 0 4.19E−05 2.11 4.3 12690 hsa-asg-6845hsa-asg-6845_st2 5.23 4.39 4.87 4.83 0.42 0 2.43 3.15 2.69 2.85 2.232.45 3.09 2.61 3.10 5.62 0.65 22 4.48E−06 2.10 4.3 hsa-asg-hsa-asg-12227_st1 4.21 4.13 3.80 4.05 0.21 0 2.18 1.21 1.92 1.97 2.782.62 2.74 1.65 0.78 7.97 0.45 100 5.87E−04 2.06 4.2 12227 hsa-miR-329hsa-asg-13589_st2 4.91 5.49 5.32 5.24 0.30 67 3.79 3.73 4.42 2.35 3.682.78 3.09 1.65 3.16 4.83 0.83 33 2.38E−03 2.06 4.2 hsa-miR-329hsa-miR-329_st1 5.31 5.14 5.35 5.27 0.11 67 4.61 3.28 4.58 2.85 2.783.32 2.74 3.76 3.02 4.63 1.28 44 1.85E−03 1.83 3.6 hsa-miR-329hsa-asg-13204_st2 4.87 4.99 5.16 5.01 0.15 33 4.21 3.40 4.24 3.48 4.002.62 3.53 4.09 2.87 4.56 0.58 33 2.59E−03 1.40 2.6 hsa-asg-hsa-asg-13308_st2 11.4 11.2 11.7 11.4 0.21 100 9.58 9.81 9.17 8.63 9.518.96 9.11 10.5 9.46 4.17 0.53 0 1.29E−04 2.05 4.1 13308 hsa-asg-4219hsa-asg-4219_st1 4.64 4.42 4.46 4.50 0.12 0 1.66 1.51 2.28 1.78 2.922.78 3.97 3.45 1.75 5.83 0.53 33 2.88E−03 2.05 4.1 hsa-miR-331hsa-asg-14205_st1 8.71 8.30 8.50 8.51 0.21 100 7.37 7.77 6.85 4.77 6.645.46 5.93 7.53 5.85 6.53 0.37 100 7.65E−03 2.04 4.1 hsa-miR-331hsa-miR-331_st2 9.78 9.64 9.85 9.76 0.10 100 8.35 8.79 7.69 7.09 7.707.28 7.20 9.04 7.57 5.19 0.93 11 1.16E−03 1.90 3.7 hsa-asg-4140hsa-asg-4140_st2 5.52 5.95 5.46 5.64 0.27 100 3.23 4.36 3.81 3.48 4.143.74 2.74 3.45 3.53 3.90 0.75 11 4.60E−05 2.03 4.1 hsa-asg-7469hsa-asg-7469_st1 5.21 4.68 5.13 5.01 0.28 0 3.14 4.86 4.45 1.29 2.482.17 3.59 2.61 2.45 5.21 0.70 56 1.47E−02 2.00 4.0 hsa-asg-3998hsa-asg-3998_st1 10.7 10.5 10.9 10.7 0.20 100 8.70 9.41 8.77 8.37 9.218.76 8.49 8.63 8.58 4.24 0.69 0 2.63E−06 1.99 4.0 hsa-asg-5304hsa-asg-5304_st1 11.4 11.3 11.6 11.5 0.15 100 9.75 10.1 9.40 8.81 9.589.07 9.15 10.6 9.48 4.05 1.09 0 1.65E−04 1.96 3.9 hsa-asg-hsa-asg-13166_st2 6.00 5.82 5.96 5.93 0.10 100 4.87 4.95 4.11 4.18 3.592.08 4.07 4.49 3.42 4.69 0.50 22 3.91E−03 1.95 3.9 13166 hsa-miR-422ahsa-miR-422a_st1 11.1 10.8 11.3 11.1 0.24 100 9.17 9.80 8.98 8.85 9.699.24 9.14 9.10 9.02 6.65 0.50 11 2.65E−06 1.92 3.8 hsa-asg-1596hsa-asg-1596_st2 5.78 6.48 6.19 6.15 0.35 100 4.02 3.21 4.39 4.18 4.144.21 4.40 4.43 5.12 7.44 0.69 89 1.15E−04 1.92 3.8 hsa-miR-369-hsa-miR-369- 5.74 5.14 5.20 5.36 0.33 100 4.47 4.36 4.36 2.53 2.92 2.623.53 3.56 2.71 9.29 0.78 89 2.80E−03 1.91 3.8 5p 5p_st2 hsa-miR-369-hsa-asg-1521_st1 4.56 4.64 4.75 4.65 0.09 0 4.02 2.23 3.72 3.73 3.402.78 3.53 3.76 3.42 4.97 0.26 11 3.86E−03 1.25 2.4 5p hsa-asg-128hsa-asg-128_st2 6.69 6.43 6.40 6.51 0.16 100 4.54 5.12 4.55 4.18 4.984.35 4.16 4.85 4.76 2.95 0.83 0 4.12E−06 1.90 3.7 hsa-miR-210hsa-miR-210_st1 9.21 7.99 8.33 8.51 0.63 100 6.84 7.08 7.11 6.71 6.116.48 7.02 6.51 5.65 4.14 2.01 22 2.67E−04 1.90 3.7 hsa-asg-9280hsa-asg-9280_st1 4.14 4.80 4.61 4.52 0.34 0 3.23 3.21 3.04 2.85 3.052.08 1.86 3.45 0.95 6.51 0.64 89 3.88E−03 1.88 3.7 hsa-miR-101hsa-miR-101_st1 7.78 6.74 7.57 7.36 0.55 100 4.21 5.98 6.52 4.70 4.864.35 5.09 7.18 6.53 2.77 1.03 0 1.85E−02 1.87 3.7 hsa-miR-409-hsa-miR-409- 6.07 5.78 5.06 5.64 0.52 0 5.14 4.99 4.77 2.99 3.96 2.623.77 2.25 3.47 5.46 0.87 22 1.58E−02 1.86 3.6 3p 3p_st1 hsa-miR-18ahsa-miR-18a_st1 7.51 6.88 7.78 7.39 0.46 100 7.10 5.97 4.53 4.14 5.695.67 4.89 5.78 6.02 2.40 0.47 0 6.91E−03 1.86 3.6 hsa-asg-9830hsa-asg-9830_st2 4.45 5.57 3.54 4.52 1.02 33 1.92 2.23 2.19 2.26 3.502.27 4.24 3.07 2.54 7.50 0.72 56 7.37E−03 1.83 3.6 hsa-asg-hsa-asg-10360_st1 4.91 5.28 5.16 5.12 0.19 67 3.06 4.66 3.72 3.26 3.172.45 2.74 3.45 3.42 3.01 1.05 0 8.17E−04 1.79 3.5 10360 hsa-miR-365hsa-cand708_st1 6.43 6.60 6.85 6.62 0.21 100 4.02 6.58 5.46 4.24 5.643.07 3.53 5.94 5.23 5.41 0.36 33 3.23E−02 1.77 3.4 hsa-miR-365hsa-miR-365_st1 7.64 7.46 7.72 7.61 0.14 100 5.53 7.63 6.38 5.40 6.664.78 4.78 7.27 5.64 4.90 0.78 22 2.71E−02 1.60 3.0 hsa-miR-365hsa-asg-13156_st1 7.57 7.47 7.55 7.53 0.05 100 6.09 7.37 6.38 5.46 6.634.78 4.67 7.33 5.87 3.35 0.83 0 3.13E−02 1.47 2.8 hsa-asg-548hsa-asg-548_st1 4.48 5.14 4.87 4.83 0.33 33 3.54 3.52 2.83 1.97 3.402.78 4.60 3.07 1.96 8.72 0.60 100 5.74E−03 1.76 3.4 hsa-miR-432hsa-miR-432_st2 6.00 7.04 6.67 6.57 0.52 100 5.92 5.53 5.77 5.68 3.504.28 2.74 4.55 5.43 3.90 0.72 11 2.98E−02 1.75 3.4 Has-miR202hsa-miR202_st2 4.85 5.60 5.20 5.21 0.38 33 4.93 3.95 3.63 2.44 4.27 2.453.09 3.85 2.71 6.76 0.45 100 8.25E−03 1.73 3.3 hsa-asg-8616hsa-asg-8616_st2 4.64 5.37 5.51 5.17 0.47 67 1.66 4.55 3.59 4.24 3.852.08 3.09 3.45 4.49 5.69 0.55 22 1.97E−02 1.73 3.3 hsa-asg-hsa-asg-14146_st1 7.77 7.19 7.70 7.55 0.31 100 6.23 6.49 5.95 4.84 5.804.68 6.48 6.30 5.77 4.74 0.70 11 1.82E−03 1.72 3.3 14146 hsa-miR-30e-hsa-miR-30e- 11.5 10.9 11.4 11.2 0.32 100 9.71 10.5 9.81 9.23 9.23 8.159.50 10.4 9.64 4.27 0.87 11 2.58E−03 1.71 3.3 5p 5p_st2 hsa-miR-422bhsa-miR-422b_st2 12.4 12.1 12.6 12.4 0.22 100 10.7 11.3 10.7 10.3 10.910.7 10.5 10.6 10.5 4.45 0.71 0 2.00E−06 1.70 3.3 hsa-asg-2419hsa-asg-2419_st2 4.03 4.17 4.46 4.22 0.22 67 3.39 2.85 2.01 1.78 1.602.45 3.25 2.61 2.71 3.97 0.61 78 1.12E−03 1.70 3.2 hsa-miR-296hsa-miR-296_st1 6.20 6.36 6.37 6.31 0.09 100 5.55 5.86 5.01 3.78 4.274.03 3.32 5.29 4.40 5.38 0.62 44 7.81E−03 1.70 3.2 hsa-miR-135bhsa-miR-135b_st2 4.91 3.49 4.46 4.29 0.72 0 2.43 2.85 2.37 3.13 3.292.27 2.92 1.45 2.71 4.80 1.13 22 1.66E−03 1.68 3.2 hsa-miR-135bhsa-cand78_st1 5.10 4.10 5.41 4.87 0.69 0 3.67 4.15 3.72 2.85 3.59 1.683.25 3.45 2.54 9.60 1.17 100 7.07E−03 1.66 3.2 hsa-miR-493- hsa-miR-493-4.91 5.03 5.38 5.11 0.24 0 4.07 3.01 2.28 1.88 4.33 3.49 4.11 4.66 3.105.28 0.49 44 1.51E−02 1.67 3.2 3p 3p_st1 hsa-miR-598 hsa-miR-598_st27.16 7.06 7.69 7.30 0.34 100 5.89 6.87 5.97 5.59 5.59 3.07 5.05 6.715.98 5.23 0.30 0 3.26E−02 1.67 3.2 hsa-miR-95 hsa-miR-95_st1 10.8 10.310.8 10.6 0.26 100 9.82 10.1 9.57 8.44 9.10 7.82 8.21 9.39 8.65 5.890.29 78 5.86E−03 1.66 3.2 hsa-asg- hsa-asg-13279_st1 8.84 8.56 9.26 8.880.35 100 7.55 8.17 7.24 6.59 6.87 7.00 6.68 7.58 7.47 7.36 0.43 564.40E−04 1.64 3.1 13279 hsa-asg- hsa-asg-14233_st1 11.0 10.6 10.9 10.80.20 100 9.23 10.1 9.44 8.89 9.05 8.87 8.89 9.45 9.26 5.63 0.32 566.70E−05 1.63 3.1 14233 hsa-miR-323 hsa-miR-323_st1 5.56 5.74 5.38 5.560.18 0 4.02 5.77 5.12 2.99 3.64 2.93 3.25 4.17 3.47 5.97 0.49 891.86E−02 1.63 3.1 hsa-cand206 hsa-cand206_st1 4.10 4.58 4.23 4.30 0.25 03.79 3.63 2.69 2.17 3.59 1.29 2.74 2.25 1.96 6.29 0.54 67 1.03E−02 1.633.1 hsa-asg-4283 hsa-asg-4283_st2 4.64 4.99 4.91 4.85 0.19 0 2.66 3.823.93 2.85 3.50 2.27 2.54 3.93 3.53 4.88 0.45 22 1.99E−03 1.62 3.1hsa-miR-410 hsa-miR-410_st1 4.73 4.17 3.54 4.15 0.60 0 1.39 3.73 2.532.85 3.17 1.88 2.54 2.43 2.36 8.08 0.42 100 4.69E−03 1.60 3.0hsa-asg-3912 hsa-asg-3912_st1 3.51 4.02 4.16 3.90 0.35 0 2.86 1.61 2.191.19 2.92 2.17 3.53 1.65 2.54 4.69 0.76 22 5.57E−03 1.60 3.0 hsa-miR-182hsa-miR-182_st1 10.5 10.7 11.3 10.8 0.42 100 8.85 9.82 9.37 9.55 8.998.57 8.13 10.2 10.0 2.47 0.48 0 4.47E−03 1.59 3.0 hsa-asg-hsa-asg-11160_st2 5.05 4.39 5.06 4.83 0.38 0 3.39 3.08 2.90 2.69 2.483.54 5.14 2.78 3.16 10.42 0.83 100 8.04E−03 1.59 3.0 11160 hsa-asg-hsa-asg-14172_st1 6.31 4.82 6.22 5.79 0.83 67 4.47 5.45 4.81 2.77 3.593.74 5.18 5.07 2.71 7.55 0.55 56 3.82E−02 1.59 3.0 14172 hsa-miR-128ahsa-miR-128a_st2 10.8 11.0 10.9 10.9 0.11 100 9.71 9.95 8.86 8.99 9.018.77 8.68 10.7 9.36 4.57 0.82 22 3.24E−03 1.59 3.0 hsa-cand631hsa-cand631_st1 5.46 4.91 5.26 5.21 0.28 33 4.12 4.71 3.85 2.17 3.932.93 3.25 4.09 3.64 5.30 0.99 22 6.16E−03 1.58 3.0 hsa-miR-127hsa-miR-127_st1 8.38 8.47 7.48 8.11 0.55 100 7.95 7.53 7.69 5.72 6.595.49 5.44 6.81 5.57 3.58 0.55 0 3.13E−02 1.58 3.0 hsa-cand330hsa-cand330_st2 5.74 5.82 5.82 5.79 0.04 100 3.54 4.71 5.15 4.18 2.643.99 3.97 4.76 5.04 3.54 0.98 0 8.22E−03 1.57 3.0 hsa-miR-379hsa-miR-379_st1 8.16 8.19 8.15 8.16 0.02 100 7.11 6.97 7.04 6.59 6.356.14 6.23 6.52 6.42 4.61 0.20 0 2.66E−05 1.57 3.0 hsa-asg-hsa-asg-8411_st2 6.50 6.01 6.39 6.30 0.26 100 5.04 6.08 5.09 4.87 4.142.93 4.07 5.92 4.49 5.29 0.28 67 2.32E−02 1.57 3.0 8411_st2 hsa-asg-hsa-asg-7472_st2 3.23 4.20 3.23 3.55 0.56 0 1.92 2.03 2.90 1.78 3.590.93 0.93 1.65 2.16 5.22 0.54 22 1.55E−02 1.56 3.0 7472_st2 hsa-asg-1619hsa-asg-1619_st2 4.64 4.91 5.13 4.89 0.25 0 2.86 4.81 3.16 2.85 2.323.07 3.25 4.37 3.29 7.41 0.81 100 7.78E−03 1.56 2.9 hsa-asg-1270hsa-asg-1270_st2 4.87 5.03 5.71 5.20 0.45 33 1.92 4.08 4.58 4.89 3.053.32 3.25 4.24 3.64 7.45 1.54 67 1.96E−02 1.54 2.9 hsa-asg-hsa-asg-13888_st2 3.17 4.36 3.88 3.80 0.60 0 1.66 3.15 1.83 2.17 2.641.88 2.74 1.45 2.87 6.12 0.45 89 3.31E−03 1.54 2.9 13888 hsa-asg-hsa-asg-14163_st2 7.53 6.56 8.43 7.51 0.93 100 6.60 7.29 5.69 5.73 6.304.91 4.40 6.59 6.24 4.98 0.47 11 2.93E−02 1.53 2.9 14163 hsa-miR-18bhsa-miR-18b_st1 7.22 6.66 7.48 7.12 0.42 100 6.71 6.01 5.09 4.68 5.714.87 5.60 6.18 5.61 5.97 0.28 56 3.96E−03 1.52 2.9 hsa-miR-128bhsa-miR-128b_st1 11.1 11.3 11.3 11.2 0.12 100 10.0 10.3 9.19 9.33 9.549.19 9.25 11.2 9.83 4.88 0.58 22 4.74E−03 1.49 2.8 hsa-asg-hsa-asg-12841_st1 4.66 4.93 4.83 4.81 0.14 0 2.18 4.15 4.04 4.10 2.713.20 1.62 4.09 3.87 7.34 1.39 100 2.72E−02 1.48 2.8 12841 hsa-asg-2967hsa-asg-2967_st2 6.40 5.99 6.16 6.18 0.21 100 4.54 4.81 5.95 4.43 3.934.35 4.32 5.18 4.86 3.84 0.86 11 2.10E−03 1.47 2.8 hsa-miR-106bhsa-miR-106b_st2 11.1 10.3 11.3 10.9 0.51 100 10.0 10.0 9.43 8.17 9.219.10 9.76 9.91 9.64 7.77 0.80 0 3.49E−03 1.47 2.8 hsa-miR-497hsa-miR-497_st1 8.70 8.10 8.65 8.48 0.33 100 6.23 8.75 7.86 6.49 6.515.62 6.69 7.43 7.60 2.14 0.48 0 3.13E−02 1.46 2.8 hsa-miR-497hsa-miR43_st1 9.41 8.93 9.47 9.27 0.29 100 7.51 9.37 8.88 7.78 7.45 7.137.83 8.20 8.56 7.80 1.03 89 2.40E−02 1.19 2.3 hsa-asg-6883hsa-asg-6883_st1 6.10 5.6 5.51 5.76 0.30 66.6 5.09 4.47 3.63 4.40 4.203.54 4.60 4.61 4.23 3.55 0.44 0 0.000709 1.45 2.74 hsa-asg-hsa-asg-14176_st1 6.98 7.23 6.61 6.94 0.31 100 5.28 6.77 6.26 5.43 5.464.21 4.16 6.06 5.72 4.66 0.23 11 2.03E−02 1.46 2.7 14176 hsa-asg-3807hsa-asg-3807_st1 4.39 4.17 5.46 4.67 0.69 0 3.06 3.95 3.89 1.68 3.402.93 3.65 3.07 3.42 5.31 0.55 67 1.01E−02 1.45 2.7 hsa-miR-140hsa-miR-140_st2 7.86 7.08 7.54 7.49 0.40 100 6.37 7.22 6.63 5.50 4.784.78 5.27 7.73 6.16 8.00 0.79 100 4.62E−02 1.45 2.7 hsa-asg-8380hsa-asg-8380_st2 5.03 5.79 5.51 5.45 0.39 67 3.91 5.56 4.58 4.18 2.782.93 4.54 4.17 3.42 6.03 0.55 78 2.21E−02 1.44 2.7 hsa-asg-9009hsa-asg-9009_st1 6.44 5.68 6.07 6.06 0.38 33 4.17 4.49 4.45 5.25 4.943.69 4.02 5.03 5.57 5.60 0.45 11 3.86E−03 1.44 2.7 hsa-asg-259hsa-asg-259_st2 5.88 5.25 6.07 5.73 0.43 100 4.54 5.04 4.42 4.18 3.293.91 3.25 5.29 4.76 5.11 0.26 0 9.20E−03 1.43 2.7 hsa-asg-8079hsa-asg-8079_st1 5.81 6.39 6.29 6.17 0.31 100 3.73 4.19 5.54 5.17 3.683.78 5.33 5.07 6.19 5.88 0.34 56 2.80E−02 1.42 2.7 hsa-asg-hsa-asg-12825_st1 4.24 4.36 6.34 4.98 1.18 33 2.43 4.49 3.77 4.55 3.773.74 3.09 3.07 3.16 2.63 0.75 0 2.61E−02 1.42 2.7 12825 hsa-asg-hsa-asg-12666_st2 6.80 6.57 5.35 6.24 0.78 100 4.87 4.91 4.65 4.60 4.823.99 4.84 6.08 4.66 11.6 0.41 100 5.42E−03 1.42 2.7 12666 hsa-miR-299-hsa-miR-299- 4.66 4.68 3.72 4.35 0.55 0 3.39 2.93 3.44 2.77 3.29 1.882.92 2.43 3.42 5.24 0.42 11 2.53E−03 1.41 2.7 3p 3p_st2 hsa-miR-299-hsa-asg-10378_st1 4.36 4.02 3.80 4.06 0.28 0 3.23 1.93 2.45 1.97 1.603.20 3.53 3.66 3.42 8.13 0.90 100 2.27E−02 1.29 2.4 3p hsa-miR-450hsa-asg-14228_st2 4.59 3.78 4.29 4.22 0.41 0 2.43 3.01 2.53 3.13 2.922.93 2.74 3.56 2.16 6.83 1.58 33 4.90E−04 1.39 2.6 hsa-miR-450hsa-miR-450_st2 5.07 4.87 4.35 4.76 0.37 0 2.86 3.91 3.63 4.03 4.20 4.474.07 4.24 2.71 5.40 0.40 67 2.99E−02 0.97 2.0 hsa-asg- hsa-asg-13471_st16.06 5.72 6.30 6.03 0.29 100 4.39 5.12 5.35 5.40 4.38 4.07 3.53 4.764.76 5.04 0.44 67 4.19E−03 1.39 2.6 13471 hsa-miR-27a hsa-miR-27a_st212.5 12.3 12.5 12.4 0.12 100 11.0 12.0 11.5 10.6 10.6 10.2 11.1 11.311.0 5.78 0.87 56 1.67E−03 1.39 2.6 hsa-asg-9547 hsa-asg-9547_st2 5.055.60 5.51 5.39 0.30 33 3.79 3.34 3.44 3.91 3.40 4.28 5.35 3.56 4.95 8.490.88 100 1.02E−02 1.39 2.6 hsa-cand22 hsa-cand22_st2 7.39 6.05 7.90 7.110.96 100 6.43 6.81 5.35 5.37 5.82 5.67 3.77 6.14 6.20 5.57 0.42 114.29E−02 1.38 2.6 hsa-asg-3711 hsa-asg-3711_st2 6.20 6.43 7.09 6.57 0.46100 5.12 5.53 5.14 5.25 4.54 4.44 4.87 5.70 6.17 4.15 0.51 78 3.11E−031.38 2.6 hsa-miR-152 hsa-miR-152_st1 12.5 12.5 12.6 12.5 0.10 100 10.912.2 11.6 11.1 10.8 9.75 10.5 12.0 11.7 8.29 0.55 100 1.54E−02 1.37 2.6hsa-miR-27b hsa-miR-27b_st1 13.6 13.5 13.7 13.6 0.10 100 12.0 13.2 12.811.9 11.9 11.0 12.5 12.5 12.3 5.21 0.40 11 5.00E−03 1.37 2.6hsa-asg-2391 hsa-asg-2391_st2 4.36 4.17 4.03 4.19 0.17 0 3.06 2.85 2.013.69 2.64 2.08 2.54 3.33 3.16 5.83 0.44 78 2.29E−03 1.37 2.6 Has-miR257hsa-miR257_st1 10.2 9.85 10.5 10.1 0.33 100 9.23 9.55 8.87 8.54 8.508.15 7.91 9.62 9.10 3.12 0.59 0 4.28E−03 1.36 2.6 hsa-miR-340hsa-miR-340_st1 6.07 5.55 6.59 6.07 0.52 100 5.24 5.71 4.89 4.46 4.493.99 2.74 5.83 4.99 4.87 0.34 0 4.18E−02 1.36 2.6 hsa-asg-360hsa-asg-360_st2 5.01 6.05 5.88 5.65 0.56 67 4.02 5.45 4.77 3.64 4.004.35 4.32 4.49 3.53 5.80 0.74 22 5.70E−03 1.36 2.6 hsa-asg-1203hsa-asg-1203_st1 4.91 5.14 5.23 5.09 0.16 33 2.66 3.82 4.21 4.55 3.933.74 1.86 4.49 4.37 10.5 0.73 100 3.14E−02 1.36 2.6 hsa-asg-hsa-asg-10679_st2 4.59 4.82 5.02 4.81 0.22 0 3.67 4.76 3.39 2.69 3.173.32 3.09 4.09 3.02 7.10 0.46 100 5.45E−03 1.34 2.5 10679 hsa-asg-9069hsa-asg-9069_st2 5.82 5.57 4.35 5.25 0.79 67 3.91 3.87 2.97 4.14 4.273.91 4.07 4.09 3.92 4.22 0.51 78 2.04E−03 1.34 2.5 Has-cand581hsa-cand581_st1 4.91 4.78 4.56 4.75 0.18 0 3.67 3.91 3.72 2.69 3.85 2.453.25 3.85 3.64 6.31 0.99 89 2.48E−03 1.30 2.5 hsa-miR-454-hsa-asg-11541_st2 9.48 8.89 9.77 9.38 0.45 100 8.53 8.45 7.87 7.92 7.917.33 7.81 8.63 8.31 6.77 0.95 78 1.09E−03 1.29 2.5 3p hsa-miR-454-hsa-asg-13305_st1 10.0 9.36 10.3 9.92 0.51 100 9.24 9.13 8.55 8.77 8.577.82 8.33 9.63 8.98 5.33 0.51 67 9.32E−03 1.14 2.2 3p hsa-asg-3821hsa-asg-3821_st2 5.47 5.57 5.48 5.51 0.05 33.3 3.38 4.63 3.09 5.11 4.204.14 4.78 3.45 5.19 4.70 0.35 0 0.018934 1.28 2.44 hsa-miR-10ahsa-miR-10a_st1 12.2 12.4 12.4 12.3 0.12 100 11.2 11.7 11.3 10.5 11.29.98 10.9 12.0 11.0 5.21 0.51 44 5.38E−03 1.29 2.4 hsa-asg-6160hsa-asg-6160_st1 3.91 3.08 2.85 3.28 0.56 0 2.18 2.77 2.11 1.01 1.251.11 2.33 3.45 1.75 4.84 0.43 11 3.03E−02 1.29 2.4 hsa-miR-148bhsa-miR-148b_st2 10.4 10.2 10.6 10.4 0.19 100 9.44 10.0 9.63 8.27 9.098.14 9.14 10.4 8.79 4.41 0.82 11 1.22E−02 1.28 2.4 hsa-miR-28hsa-miR-28_st1 11.7 11.5 11.8 11.7 0.16 100 10.0 11.0 10.8 10.1 10.19.40 10.5 11.1 10.7 4.60 1.26 0 4.02E−03 1.27 2.4 hsa-miR-324-hsa-miR-324- 9.16 9.00 9.49 9.22 0.25 100 8.33 8.27 7.80 7.42 7.82 7.877.98 7.92 8.13 5.22 0.58 11 3.47E−05 1.27 2.4 5p 5p_st1 hsa-asg-1221hsa-asg-1221_st2 4.87 5.21 4.35 4.81 0.44 33 4.39 4.23 3.77 3.13 2.642.93 3.53 4.02 3.29 4.60 0.41 0 7.81E−03 1.26 2.4 hsa-asg-hsa-asg-13350_st2 9.37 9.75 10.5 9.88 0.58 100 8.31 8.84 8.61 8.88 8.308.27 8.07 9.29 9.08 6.10 0.99 67 2.06E−03 1.25 2.4 13350 hsa-miR-99bhsa-miR-99b_st1 11.8 11.5 11.9 11.7 0.21 100 10.5 11.4 11.1 10.1 10.19.52 10.3 11.0 10.5 3.02 0.78 0 6.10E−03 1.24 2.4 hsa-miR-99bhsa-asg-14196_st2 11.9 11.7 12.0 11.9 0.18 100 10.6 11.6 11.3 10.3 10.39.55 10.5 11.2 10.6 8.67 0.42 100 1.05E−02 1.22 2.3 hsa-asg-hsa-asg-14048_st1 5.40 5.86 5.29 5.52 0.30 67 3.54 3.63 5.03 3.26 4.595.15 4.07 4.43 4.95 5.31 0.50 22 1.73E−02 1.22 2.3 14048 hsa-miR-641hsa-miR-641_st1 4.80 3.69 4.46 4.32 0.57 0 3.79 2.77 2.19 3.54 3.68 2.622.33 4.09 2.87 4.70 0.67 22 2.11E−02 1.22 2.3 hsa-asg-9050hsa-asg-9050_st1 8.02 8.50 9.64 8.72 0.83 100 6.93 7.54 7.54 7.69 6.717.56 7.31 8.14 8.14 2.75 0.72 0 9.54E−03 1.21 2.3 Has-cand821hsa-cand821_st1 4.78 4.48 4.75 4.67 0.17 0 3.23 4.00 4.24 3.37 2.64 3.443.39 3.76 3.02 8.90 0.56 100 2.16E−03 1.21 2.3 hsa-miR-132hsa-miR-132_st1 9.20 9.43 9.98 9.54 0.41 100 8.33 8.35 8.59 8.08 8.028.44 6.88 9.72 8.56 2.91 0.77 0 2.42E−02 1.21 2.3 hsa-asg-9951hsa-asg-9951_st2 5.52 5.14 4.91 5.19 0.30 67 3.91 4.95 4.39 3.59 1.974.47 3.87 4.81 3.92 5.32 0.37 11 4.81E−02 1.20 2.3 hsa-miR-19bhsa-miR-19b_st2 8.81 8.31 8.88 8.67 0.31 100 7.31 8.03 8.04 6.51 6.916.53 7.20 8.38 8.32 2.14 0.64 0 2.41E−02 1.20 2.3 hsa-miR-514hsa-miR-514_st1 3.29 2.73 2.85 2.96 0.29 0 2.43 1.72 1.11 1.58 0.91 2.452.10 2.05 1.54 5.09 0.50 11 5.16E−03 1.19 2.3 hsa-miR-374hsa-miR-374_st2 8.29 6.84 8.15 7.76 0.80 100 6.51 8.11 7.01 5.65 6.505.99 6.37 7.01 5.98 5.35 0.55 33 3.86E−02 1.19 2.3 hsa-miR-382hsa-miR-382_st1 7.58 8.10 8.04 7.91 0.29 100 7.35 7.13 7.43 6.56 6.295.93 5.81 7.17 6.84 10.9 0.36 100 9.71E−03 1.19 2.3 hsa-miR-154*hsa-miR-154-_st2 5.07 6.07 5.41 5.51 0.51 67 4.21 4.99 4.63 3.95 4.273.20 4.40 5.83 3.53 4.28 0.68 0 3.63E−02 1.18 2.3 hsa-miR-599hsa-miR-599_st2 4.48 4.68 4.65 4.61 0.11 0 3.39 4.71 3.44 3.37 4.07 1.882.92 3.66 3.53 9.79 0.58 89 3.04E−02 1.16 2.2 hsa-asg-8511hsa-asg-8511_st2 3.56 4.45 5.57 4.52 1.01 0 2.86 3.57 1.92 3.78 2.924.07 3.53 3.66 3.92 7.59 0.85 89 4.32E−02 1.16 2.2 hsa-asg-hsa-asg-11688_st1 4.36 4.30 3.80 4.15 0.31 0 2.66 2.33 2.61 4.10 2.323.83 2.10 3.33 3.92 6.80 1.32 100 3.78E−02 1.13 2.2 11688 hsa-asg-3340hsa-asg-3340_st2 7.72 7.45 9.04 8.07 0.85 100 6.10 6.92 7.19 6.82 6.606.86 6.89 7.52 7.52 4.36 0.65 22 1.16E−02 1.13 2.2 hsa-asg-hsa-asg-13074_st2 5.37 4.58 4.75 4.90 0.42 33 3.67 4.66 2.97 3.26 3.403.83 3.65 4.49 4.00 3.80 1.12 0 9.27E−03 1.13 2.2 13074 hsa-asg-hsa-asg-13646_st1 8.96 7.96 8.22 8.38 0.52 100 6.97 7.98 6.55 7.56 6.686.07 7.12 8.24 8.13 1.96 0.68 0 4.20E−02 1.12 2.2 13646 hsa-miR-494hsa-miR-494_st1 7.61 7.92 7.92 7.81 0.18 100 7.22 6.96 6.63 7.66 5.735.86 6.60 6.40 7.30 10.7 0.67 100 1.77E−02 1.11 2.2 hsa-miR-494hsa-miR61_st2 7.01 7.33 7.50 7.28 0.25 100 6.85 6.43 6.38 7.10 5.40 5.796.89 6.83 6.55 2.11 0.39 0 3.77E−02 0.81 1.8 hsa-asg-9053hsa-asg-9053_st1 6.17 7.44 7.34 6.98 0.71 100 4.78 6.21 6.10 6.92 4.905.92 5.64 5.87 6.55 3.38 0.87 0 3.96E−02 1.10 2.1 hsa-asg-hsa-asg-10336_st1 5.23 5.68 4.83 5.24 0.42 33 3.54 4.33 4.63 3.82 4.383.74 4.07 4.76 4.00 5.74 0.33 56 2.58E−03 1.10 2.1 10336 hsa-asg-hsa-asg-12563_st1 5.79 4.68 4.46 4.98 0.71 33 4.54 2.85 4.47 3.13 3.683.44 3.97 4.17 4.61 2.20 0.54 0 2.99E−02 1.10 2.1 12563 hsa-asg-hsa-asg-10513_st1 5.34 5.28 4.75 5.12 0.33 67 4.47 4.71 5.19 2.69 3.403.74 3.65 4.66 3.74 5.98 0.52 89 4.44E−02 1.09 2.1 10513 hsa-asg-7417hsa-asg-7417_st1 5.72 4.45 4.89 5.02 0.65 0 4.50 5.28 4.04 3.26 3.593.59 3.17 4.55 3.36 4.55 1.32 22 4.26E−02 1.09 2.1 hsa-asg-807hsa-asg-807_st2 5.94 5.79 6.17 5.97 0.19 100 4.54 4.36 4.74 5.57 4.595.00 5.56 4.17 5.46 5.37 0.68 11 7.57E−03 1.08 2.1 hsa-asg-hsa-asg-10138_st1 9.27 9.62 10.3 9.74 0.54 100 8.28 9.01 8.62 8.80 8.078.36 8.44 9.36 9.10 4.91 0.48 56 5.20E−03 1.07 2.1 10138 hsa-miR-421hsa-asg-5033_st2 9.31 8.84 9.67 9.27 0.42 100 8.48 8.62 7.79 8.03 7.788.78 8.07 8.22 8.12 4.26 0.56 0 1.43E−03 1.06 2.1 hsa-miR-421hsa-miR-421-3p_st1 10.0 9.52 10.4 9.99 0.46 100 8.98 9.42 8.71 8.84 8.379.39 8.91 9.50 9.20 4.25 0.48 0 4.64E−03 0.95 1.9 hsa-cand279hsa-cand279_st2 5.46 5.78 6.69 5.98 0.64 67 5.12 4.93 4.93 4.75 4.545.17 4.63 5.42 4.74 8.27 0.74 22 1.86E−03 1.06 2.1 hsa-asg-6331hsa-asg-6331_st2 7.68 7.59 8.77 8.01 0.65 100 6.59 7.05 5.98 7.23 6.427.03 7.36 7.18 7.74 5.65 0.65 89 1.79E−02 1.06 2.1 hsa-asg-hsa-asg-12465_st1 5.73 5.60 5.46 5.60 0.14 67 4.21 5.39 5.05 4.46 4.493.20 4.67 4.81 4.61 8.83 0.96 100 1.63E−02 1.06 2.1 12465 hsa-asg-hsa-asg-12515_st2 4.51 5.33 5.35 5.06 0.48 0 3.06 4.12 4.60 4.37 3.684.14 3.77 4.02 4.30 4.75 0.59 22 6.53E−03 1.05 2.1 12515 hsa-asg-510hsa-asg-510_st1 6.36 5.88 6.34 6.20 0.27 100 4.75 6.06 5.19 4.63 4.905.26 4.54 6.02 4.95 5.68 0.40 67 1.20E−02 1.05 2.1 hsa-miR-134hsa-miR-134_st2 6.43 6.39 5.90 6.24 0.29 67 5.99 5.85 5.70 4.21 4.734.31 5.02 5.11 5.78 4.95 0.40 11 2.94E−02 1.05 2.1 hsa-asg-6803hsa-asg-6803_st1 4.82 5.28 5.13 5.08 0.23 67 3.67 4.49 4.63 4.18 3.173.54 4.16 5.03 3.42 5.48 0.50 11 1.94E−02 1.05 2.1 hsa-asg-hsa-asg-10830_st2 4.91 5.07 4.91 4.96 0.09 0 3.67 3.91 3.93 4.49 3.403.64 3.87 4.37 4.00 6.40 2.06 78 5.19E−04 1.04 2.1 10830 hsa-miR-30a-hsa-miR-30a-5p_st2 12.9 12.7 13.0 12.9 0.13 100 11.9 12.5 12.1 11.7 11.510.8 11.8 12.2 12.1 7.76 0.69 100 4.79E−03 1.04 2.1 5p hsa-asg-hsa-asg-10626_st2 5.72 6.21 5.75 5.89 0.27 100 4.61 5.37 4.99 4.31 4.864.28 5.00 5.14 5.19 9.80 0.95 100 1.73E−03 1.03 2.0 10626 hsa-asg-hsa-asg-14138_st2 5.90 5.95 5.94 5.93 0.03 33 5.35 5.50 4.91 5.32 4.143.95 4.57 5.78 4.58 5.39 0.49 33 2.10E−02 1.03 2.0 14138 hsa-asg-hsa-asg-13011_st2 5.60 5.28 6.16 5.68 0.44 100 4.54 5.96 4.85 4.49 4.333.99 4.16 5.03 4.55 8.03 0.95 100 2.08E−02 1.02 2.0 13011 hsa-asg-4513hsa-asg-4513_st2 5.14 4.95 4.29 4.79 0.45 0 4.30 3.01 3.44 4.18 4.143.44 3.77 3.21 4.49 5.56 0.48 22 1.37E−02 1.02 2.0 hsa-asg-3224hsa-asg-3224_st1 5.37 5.34 4.91 5.21 0.26 67 4.47 4.49 4.42 4.37 3.503.64 4.32 4.81 3.83 8.18 0.65 100 4.38E−03 1.00 2.0 hsa-miR-324-hsa-miR-324- 8.32 8.09 8.66 8.36 0.29 0 7.35 8.09 7.56 6.92 7.41 6.657.27 7.66 7.30 7.37 0.66 44 3.35E−03 1.00 2.0 3p 3p_st1 SD, standarddeviation; %, percentage of samples expressing miRNA above backgroundlevels; Log2Diff, difference in VSN-transformed expression between ESOand LNneg samples.

Example 22 miRNAs Differentally Expressed Between Normal Liver Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous liver cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal liver tissuethan in cancer-negative lymph nodes.

Total RNA, purified from three separate normal human liver tissuesamples (FirstChoice® Human Liver Total RNA, cat. no. AM7960; Ambion,Austin, Tex., USA) and from nine normal human lymph node samples(FirstChoice® Human Lymph Node Total RNA, cat. no. AM7894; Ambion,Austin, Tex., USA) was purchased from Ambion. miRNA purification andlabeling and microarray expression analyses were performed as describedabove in Example 2.

The inventors identified 198 miRNAs (51 verified, 147 predicted) withsignificant differential expression between normal liver and normallymph node samples (log 2Diff liver vs LN neg≧1 and p-value<0.05) (Table21). Among the verified miRNAs, hsa-miR-122a had an average expressionlevel over 300-fold higher in liver samples; hsa-miR-375 had an averageexpression level over 50-fold higher in liver samples; four miRNAs(hsa-miR-215, -194, -483, and -192) had average expression levels thatwere between 15- and 25-fold higher in liver samples; and nine miRNAs(hsa-miR-200b, -592, -429, -139, -203, -200a, -19a, -148a, and -493-5p)had average expression levels that were between 5- and 10-fold higher inliver samples. Among the predicted miRNAs, hsa-asg-14182_st1 had anaverage expression level over 40-fold higher in liver samples; eightmiRNAs (hsa-asg-1035_st2, -9663_st1, -11391_st1, -3922_st2, -2303_st1,-14208 st1, -11425_st1, -and -8128 st1) had average expression levelsthat were between 10- and 30-fold higher in liver samples; and 30additional miRNAs had average expression levels that were between 5- and10-fold higher in liver samples than in normal lymph node samples.

TABLE 21 MicroRNAs Differentially Expressed Between Normal Liver Samples(LIV) and Cancer-Negative Lymph Node Samples (LNneg) from Patients. LIVLNneg MicroRNA Probe ID 1 2 3 M SD % 1 2 3 4 5 6 7 8 9 M SD % ttestlog2Diff Fold hsa-miR-122a hsa-miR-122a_st1 14.4 14.5 14.5 14.5 0.06 1008.57 6.20 6.41 4.26 7.93 3.82 1.42 4.58 10.8 6.01 2.85 44 5.45E−04 8.49360. hsa-miR- hsa-miR- 10.1 11.4 9.89 10.4 0.83 100 5.96 4.93 6.23 2.852.97 3.69 0.99 4.63 6.39 4.29 1.82 0 2.41E−04 6.19 72.9 375 375_st1hsa-asg- hsa-asg- 6.74 8.10 7.59 7.48 0.69 100 2.43 0.95 1.92 1.82 2.611.78 2.78 1.88 0.73 1.88 0.69 0 2.66E−07 5.60 48.3 14182 14182_st1hsa-asg- hsa-asg- 7.20 7.10 7.12 7.14 0.05 100 3.07 1.54 3.23 2.69 2.611.97 2.15 1.88 2.92 2.45 0.59 0 1.06E−07 4.69 25.8 1035 1035_st2hsa-asg- hsa-asg- 6.83 6.71 7.11 6.88 0.21 100 2.25 1.96 1.66 1.31 2.972.35 1.97 2.93 3.39 2.31 0.68 0 5.61E−07 4.57 23.8 9663 9663_st1hsa-miR- hsa-miR- 10.0 10.5 10.0 10.2 0.27 100 6.96 5.82 5.71 6.66 5.544.84 5.51 5.11 5.23 5.71 0.70 33 9.04E−07 4.50 22.7 215 215_st1 hsa-miR-hsa-miR- 13.0 13.3 13.2 13.2 0.14 100 9.83 9.37 8.91 9.35 8.95 8.34 8.558.21 9.05 8.95 0.52 100 9.09E−08 4.28 19.4 194 194_st2 hsa-miR- hsa-miR-6.73 7.05 6.85 6.88 0.16 100 1.85 2.45 1.79 4.08 2.76 1.29 3.68 3.872.44 2.69 0.99 0 3.55E−05 4.19 18.2 483 483_st1 hsa-asg- hsa-asg- 6.286.02 6.22 6.17 0.14 100 1.45 1.54 2.43 2.03 3.44 1.88 2.64 2.27 1.622.14 0.64 0 9.44E−07 4.03 16.3 11391 11391_st1 hsa-miR- hsa-miR- 13.513.7 13.7 13.6 0.11 100 10.8 9.87 10.1 10.0 9.58 8.72 9.33 8.80 9.659.66 0.66 100 1.43E−06 4.01 16.1 192 192_st2 hsa-asg-3922hsa-asg-3922_st2 5.63 6.00 6.74 6.12 0.56 100 2.25 2.36 1.66 2.33 2.011.97 2.92 2.62 2.54 2.30 0.38 0 9.14E−08 3.83 14.2 hsa-asg- hsa-asg-6.16 5.74 6.06 5.99 0.22 100 2.93 2.54 2.43 1.82 2.11 2.99 2.32 1.292.54 2.33 0.53 0 5.34E−07 3.66 12.6 2303 2303_st1 hsa-asg- hsa-asg- 7.177.30 7.45 7.31 0.14 100 5.07 3.53 4.47 4.15 3.39 1.19 3.05 4.07 3.973.65 1.10 0 2.42E−04 3.65 12.6 14208 14208_st1 hsa-asg- hsa-asg- 5.535.64 6.00 5.72 0.25 100 1.65 2.16 1.92 2.60 2.37 1.10 2.64 2.08 3.092.18 0.59 0 1.81E−06 3.54 11.6 11425 11425_st1 hsa-miR- hsa-asg- 6.975.94 5.91 6.28 0.60 100 3.07 3.29 3.67 1.72 1.64 2.69 3.05 1.68 5.422.92 1.21 11 1.09E−03 3.36 10.3 592 4400_st1 hsa-miR-592 hsa-miR-592_st16.84 5.25 6.18 6.09 0.80 100 4.31 2.54 2.86 3.15 1.83 1.78 3.05 2.624.54 2.96 0.96 0 4.94E−04 3.13 8.7 hsa-asg- hsa-asg- 5.58 6.34 6.63 6.180.54 67 3.45 4.04 3.46 3.01 3.39 2.92 2.15 0.84 2.21 2.83 0.96 02.20E−04 3.35 10.2 8128 8128_st1 hsa-asg- hsa-asg- 6.69 5.62 5.96 6.090.55 33 4.17 2.71 5.28 2.93 3.28 1.58 2.15 1.11 2.33 2.84 1.29 02.00E−03 3.26 9.5 13404 13404_st2 hsa-asg- hsa-asg- 4.53 5.15 5.80 5.160.63 33 2.61 2.54 2.66 2.13 1.83 0.68 1.97 0.93 1.86 1.91 0.71 03.51E−05 3.25 9.5 519 519_st1 hsa-asg- hsa-asg- 5.98 6.34 6.16 6.16 0.18100 2.78 2.36 2.66 2.03 3.97 2.92 2.92 2.78 4.24 2.96 0.71 0 2.09E−053.20 9.2 7438 7438_st2 hsa-miR-200b hsa-miR-200b_st1 10.6 11.2 10.6 10.80.35 100 7.62 7.80 8.90 6.69 6.82 6.87 6.51 7.65 10.4 7.70 1.26 1001.87E−03 3.18 9.1 hsa-asg- hsa-asg- 4.77 5.18 6.24 5.40 0.76 33 1.451.75 3.06 1.72 2.11 1.78 1.25 3.20 3.65 2.22 0.86 0 2.05E−04 3.18 9.111825 11825_st1 hsa-miR- hsa-miR- 5.68 6.28 5.89 5.95 0.30 100 3.45 1.543.67 2.13 2.37 2.69 2.32 2.45 5.39 2.89 1.14 0 1.20E−03 3.06 8.3 429429_st1 hsa-asg- hsa-asg- 4.68 6.09 6.22 5.67 0.85 67 2.78 2.71 1.662.42 3.97 3.06 2.78 1.29 3.25 2.66 0.81 0 2.50E−04 3.01 8.0 86118611_st1 hsa-asg- hsa-asg- 6.20 6.43 6.60 6.41 0.20 100 3.45 4.49 1.664.55 3.59 4.24 3.85 2.08 3.09 3.44 1.02 0 6.70E−04 2.97 7.8 86168616_st2 hsa-asg- hsa-asg- 5.55 6.16 6.22 5.98 0.37 100 4.55 3.16 3.913.28 2.01 3.69 3.50 1.88 1.38 3.04 1.05 0 9.74E−04 2.94 7.7 1261012610_st1 hsa-asg- hsa-asg- 5.50 6.45 6.81 6.26 0.68 100 4.37 3.29 2.864.81 3.16 2.85 2.32 3.07 3.25 3.33 0.78 0 1.80E−04 2.92 7.6 16191619_st2 hsa-asg- hsa-asg- 5.26 6.49 6.71 6.15 0.78 67 2.93 3.53 2.864.84 4.27 3.37 3.05 2.45 1.86 3.24 0.90 0 5.62E−04 2.91 7.5 1387813878_st2 hsa-miR- hsa-miR- 11.8 11.9 11.5 11.7 0.16 100 11.0 9.41 9.559.74 8.45 8.99 9.36 7.36 5.92 8.86 1.48 100 8.17E−03 2.90 7.5 139139_st2 hsa-asg-1270 hsa-asg-1270_st2 5.84 6.99 6.84 6.56 0.63 100 4.243.64 1.92 4.08 4.58 4.89 3.05 3.32 3.25 3.66 0.90 0 4.82E−04 2.89 7.4hsa-asg- hsa-asg- 5.58 6.02 5.00 5.53 0.51 67 3.76 2.54 2.18 2.69 2.763.37 1.79 2.27 2.54 2.66 0.60 0 2.40E−05 2.88 7.3 5190 5190_st1 hsa-asg-hsa-asg- 5.73 6.01 5.55 5.76 0.23 33 3.56 3.02 3.23 2.60 3.04 3.69 1.083.20 2.74 2.91 0.77 0 1.05E−04 2.86 7.2 11995 11995_st1 hsa-miR-hsa-miR- 10.0 9.38 9.52 9.65 0.35 100 7.33 5.89 7.21 8.24 6.45 5.41 5.196.07 9.36 6.79 1.37 78 6.11E−03 2.86 7.2 203 203_st2 hsa-miR- hsa-miR-8.05 8.74 8.45 8.41 0.35 100 6.27 5.08 6.49 5.72 5.29 4.60 4.07 4.787.78 5.56 1.14 67 1.97E−03 2.85 7.2 200a 200a_st2 hsa-asg- hsa-asg- 5.535.94 5.23 5.57 0.36 100 3.76 2.87 3.54 2.51 2.83 1.97 2.78 1.68 2.542.72 0.66 0 3.82E−05 2.84 7.2 12907 12907_st2 hsa-asg- hsa-asg- 5.606.33 6.36 6.10 0.43 67 3.66 3.53 1.66 5.10 3.77 3.69 3.59 2.62 2.54 3.350.97 0 9.45E−04 2.75 6.7 10947 10947_st1 hsa-asg- hsa-asg- 5.26 6.136.47 5.95 0.62 67 3.93 3.53 2.66 3.82 3.93 2.85 3.50 2.27 2.54 3.22 0.650 8.29E−05 2.73 6.6 4283 4283_st2 Has- hsa- 6.31 6.20 6.02 6.18 0.15 1003.85 2.71 4.93 3.95 3.63 2.44 4.27 2.45 3.09 3.48 0.87 0 3.96E−04 2.706.5 miR202 miR202_st2 hsa-miR- hsa-miR- 5.09 5.92 5.50 5.50 0.41 67 4.613.42 2.86 3.15 3.63 2.53 1.60 1.29 2.54 2.85 1.02 0 1.59E−03 2.66 6.319a 19a_st1 hsa-asg- hsa-asg- 6.93 7.52 7.34 7.27 0.30 100 5.18 4.436.99 6.06 5.91 3.54 4.59 3.07 2.10 4.65 1.57 22 1.93E−02 2.61 6.15021_st1 5021_st1 hsa-asg- hsa-asg- 5.70 6.77 6.81 6.43 0.63 67 4.554.16 3.23 5.06 5.07 4.55 3.59 2.45 2.10 3.86 1.09 0 3.50E−03 2.57 5.98668 8668_st2 hsa-asg-7159 hsa-asg-7159_st2 5.55 6.36 5.84 5.92 0.41 333.56 3.29 2.43 3.95 3.93 3.48 4.00 3.44 2.33 3.38 0.62 0 6.79E−05 2.545.8 Has- hsa- 6.13 6.97 7.02 6.71 0.50 100 4.76 5.04 3.54 4.71 5.15 4.182.64 3.99 3.97 4.22 0.80 11 5.70E−04 2.49 5.6 cand330 cand330_st2hsa-asg- hsa-asg- 6.04 6.14 6.79 6.32 0.41 100 4.71 5.40 1.66 4.84 5.585.17 4.07 2.27 1.38 3.90 1.67 0 3.61E−02 2.43 5.4 13922 13922_st1hsa-miR- hsa-miR- 12.8 13.1 13.0 13.0 0.13 100 12.1 11.1 11.5 10.5 10.211.0 10.2 9.18 9.38 10.6 0.96 100 1.85E−03 2.42 5.3 148a 148a_st1hsa-asg- hsa-asg- 5.67 6.62 6.33 6.21 0.49 67 4.49 4.27 2.76 4.93 4.535.27 2.32 2.53 3.17 3.81 1.11 0 5.46E−03 2.40 5.3 3185 3185_st1hsa-asg-12666 hsa-asg-12666_st2 7.19 7.54 6.93 7.22 0.31 100 6.08 4.664.87 4.91 4.65 4.60 4.82 3.99 4.84 4.83 0.55 11 3.38E−05 2.40 5.3hsa-miR- hsa-miR-493- 7.00 7.32 6.92 7.08 0.21 100 4.37 3.83 6.88 6.186.33 3.69 4.38 1.88 4.60 4.68 1.56 33 2.81E−02 2.40 5.3 493-5p 5p_st1hsa-asg- hsa-asg- 4.18 5.66 5.91 5.25 0.94 67 3.76 4.23 2.18 2.85 2.012.99 2.78 2.62 2.54 2.89 0.71 0 8.92E−04 2.37 5.2 2844 2844_st1hsa-asg-13471 hsa-asg-13471_st1 6.36 7.27 7.36 7.00 0.56 100 4.76 4.764.39 5.12 5.35 5.40 4.38 4.07 3.53 4.64 0.61 11 1.59E−04 2.36 5.1hsa-asg- hsa-asg- 6.69 6.02 7.31 6.67 0.64 100 4.17 3.83 4.61 5.23 4.454.75 4.49 3.44 3.87 4.32 0.55 0 9.73E−05 2.36 5.1 8266 8266_st2hsa-asg-1268 hsa-asg-1268_st1 6.20 5.86 6.06 6.04 0.17 100 4.76 3.294.02 4.15 3.49 3.78 3.85 2.27 3.65 3.70 0.68 0 1.93E−04 2.35 5.1hsa-asg- hsa-asg- 7.06 7.58 7.06 7.23 0.30 100 5.60 5.37 6.65 5.89 5.904.03 4.73 2.45 3.39 4.89 1.36 56 1.68E−02 2.34 5.1 13254 13254_st1hsa-asg- hsa-asg- 5.26 6.67 5.77 5.90 0.71 67 3.07 3.16 2.43 4.49 3.774.55 3.77 3.74 3.09 3.56 0.69 0 5.15E−04 2.34 5.1 12825 12825_st1hsa-asg- hsa-asg- 5.96 5.71 5.43 5.70 0.26 33 3.93 4.63 1.79 1.31 5.455.35 1.25 3.49 3.17 3.37 1.64 0 3.90E−02 2.33 5.0 13498 13498_st1hsa-asg- hsa-asg- 5.92 6.09 5.91 5.98 0.10 67 4.02 3.83 4.39 3.87 3.723.54 2.92 2.93 3.77 3.66 0.48 0 1.11E−05 2.31 5.0 6642 6642_st2 hsa-asg-hsa-asg- 7.69 6.89 7.36 7.32 0.40 100 6.35 4.81 6.11 6.00 4.42 5.17 4.204.83 3.25 5.02 1.01 56 3.86E−03 2.30 4.9 2381 2381_st2 hsa-asg- hsa-asg-5.16 6.48 6.41 6.02 0.74 67 4.49 4.37 2.66 3.82 4.21 4.55 3.93 3.74 1.863.74 0.91 0 2.93E−03 2.28 4.9 1203 1203_st1 hsa-asg- hsa-asg- 5.20 5.925.40 5.51 0.37 33 3.76 4.00 2.86 2.93 3.63 4.34 2.92 1.88 2.74 3.23 0.760 6.57E−04 2.28 4.8 4472 4472_st2 hsa-asg- hsa-asg- 4.42 5.71 5.72 5.280.74 33 2.93 3.83 3.06 3.68 2.01 3.19 3.05 2.78 2.54 3.01 0.55 01.87E−04 2.28 4.8 2845 2845_st2 hsa-asg- hsa-asg- 6.88 7.28 6.89 7.020.23 100 5.11 4.76 4.81 5.06 4.36 4.68 4.82 4.52 4.73 4.76 0.23 04.68E−08 2.25 4.8 10775_st2 10775_st2 hsa-asg- hsa-asg- 4.48 5.80 4.494.92 0.76 33 2.78 2.36 3.39 2.33 3.04 2.99 3.29 2.27 2.10 2.73 0.48 01.24E−04 2.20 4.6 11040 11040_st2 hsa-asg- hsa-asg- 5.23 6.27 6.52 6.010.68 67 4.09 3.53 3.06 4.52 5.17 3.78 3.17 3.74 3.39 3.83 0.68 07.05E−04 2.18 4.5 10236 10236_st2 hsa-asg- hsa-asg- 5.88 5.87 6.20 5.980.19 67 3.66 2.54 4.47 3.46 3.93 4.03 3.29 3.64 5.27 3.81 0.76 07.94E−04 2.18 4.5 7637 7637_st2 hsa-miR- hsa-miR- 7.70 8.03 7.59 7.770.23 100 6.06 5.84 6.97 6.90 6.25 5.40 4.90 3.74 4.40 5.61 1.10 678.28E−03 2.17 4.5 487b 487b_st2 hsa-asg- hsa-asg- 4.08 6.34 4.34 4.921.24 33 1.25 2.63 5.30 4.63 1.83 2.53 2.92 1.39 2.44 2.77 1.38 03.83E−02 2.15 4.4 14065 14065_st2 hsa-asg- hsa-asg- 4.98 5.22 4.66 4.950.28 33 3.76 3.29 3.91 3.63 3.44 2.69 1.42 1.48 1.62 2.80 1.03 06.11E−03 2.15 4.4 5617 5617_st1 hsa-asg- hsa-asg- 5.36 6.24 6.86 6.150.76 67 4.02 4.30 3.06 4.12 4.60 4.37 3.68 4.14 3.77 4.01 0.46 01.22E−04 2.15 4.4 12515 12515_st2 hsa-asg- hsa-asg- 6.68 6.32 7.68 6.900.71 100 5.98 5.12 3.23 5.27 6.03 5.05 4.54 3.91 3.97 4.79 0.95 445.99E−03 2.11 4.3 13077 13077_st1 hsa-asg- hsa-asg- 6.97 6.98 7.53 7.160.32 100 5.75 5.99 4.78 5.25 5.49 6.26 3.05 5.17 3.92 5.07 1.03 227.06E−03 2.09 4.3 14148 14148_st2 hsa-asg- hsa-asg- 6.76 7.23 7.21 7.070.26 100 5.94 4.99 4.12 6.59 6.41 5.63 3.77 3.07 4.32 4.98 1.24 331.87E−02 2.08 4.2 4542 4542_st2 hsa-miR- hsa-miR- 9.87 10.2 10.2 10.10.22 100 9.76 7.76 8.97 9.69 7.39 7.36 7.88 7.63 6.14 8.06 1.19 891.62E−02 2.06 4.2 505 505_st1 hsa-asg- hsa-asg- 5.86 5.59 5.40 5.62 0.23100 4.71 4.16 4.54 3.63 3.44 3.59 3.40 2.27 2.92 3.63 0.77 0 1.59E−031.99 4.0 2299 2299_st1 hsa- hsa- 10.3 10.7 10.4 10.4 0.21 100 9.47 8.169.72 9.39 8.42 7.95 8.50 7.61 7.41 8.51 0.84 100 2.86E−03 1.98 3.9miR157 miR157_st2 hsa-asg- hsa-asg- 7.26 6.99 8.41 7.55 0.75 100 5.605.72 5.67 6.74 5.70 5.30 5.25 5.00 6.09 5.68 0.51 56 5.74E−04 1.88 3.77471 7471_st1 hsa-miR- hsa-asg- 5.39 5.64 5.34 5.45 0.16 100 3.33 4.303.39 4.00 4.85 3.69 2.92 4.07 1.86 3.60 0.87 0 5.23E−03 1.85 3.6 376a14234_st1 hsa-miR- hsa-miR- 5.90 6.26 6.06 6.08 0.18 100 5.14 4.08 5.495.67 4.85 4.03 2.48 3.83 3.77 4.37 1.01 0 1.84E−02 1.70 3.3 376a376a_st2 hsa-miR- hsa-asg- 6.40 6.55 6.50 6.48 0.07 100 5.03 4.91 6.095.56 5.35 4.10 3.85 4.14 4.67 4.85 0.74 11 4.22E−03 1.63 3.1 376a10049_st1 hsa-asg- hsa-asg- 5.53 5.51 6.46 5.83 0.54 100 4.76 4.49 3.234.30 5.75 4.55 3.77 3.91 1.38 4.01 1.21 11 3.44E−02 1.82 3.5 30813081_st1 hsa-asg- hsa-asg- 5.73 6.94 7.23 6.63 0.80 100 5.45 5.08 3.545.28 5.43 5.03 4.64 4.58 4.40 4.83 0.61 11 2.02E−03 1.81 3.5 48404840_st1 hsa-asg- hsa-asg- 5.47 5.04 5.50 5.34 0.25 33 4.61 3.92 4.812.51 4.00 2.17 3.68 2.27 3.87 3.54 0.99 0 1.27E−02 1.80 3.5 74647464_st2 hsa-asg- hsa-asg- 7.74 6.88 8.42 7.68 0.77 100 6.16 6.49 6.216.31 4.24 7.42 5.73 4.83 5.75 5.90 0.93 78 1.44E−02 1.78 3.4 15191519_st1 hsa-miR- hsa-miR- 7.15 7.34 7.25 7.25 0.09 100 7.18 6.53 4.215.98 6.52 4.70 4.86 4.35 5.09 5.49 1.08 44 2.15E−02 1.76 3.4 101 101_st1hsa-miR- hsa-miR-369- 5.65 5.04 4.91 5.20 0.40 33 3.56 2.71 4.47 4.364.36 2.53 2.92 2.62 3.53 3.45 0.80 0 5.06E−03 1.75 3.4 369-5p 5p_st2hsa-miR- hsa-miR- 6.30 6.84 6.56 6.57 0.27 100 4.55 5.43 5.92 5.53 5.775.68 3.50 4.28 2.74 4.82 1.13 22 2.76E−02 1.74 3.4 432 432_st2 hsa-asg-hsa-asg- 7.13 7.35 7.18 7.22 0.12 100 6.06 5.72 5.28 6.77 6.26 5.43 5.464.21 4.16 5.48 0.87 44 7.57E−03 1.74 3.3 14176 14176_st1 hsa-asg-hsa-asg- 5.43 6.34 5.88 5.88 0.45 33 4.31 4.93 2.76 4.46 4.30 4.97 4.143.59 3.92 4.15 0.68 0 2.38E−03 1.73 3.3 4069 4069_st1 hsa-miR-92bhsa-miR-92b_st1 11.0 11.3 10.9 11.0 0.19 100 10.9 8.50 10.1 10.2 9.257.88 9.75 9.23 8.88 9.42 0.94 100 1.41E−02 1.67 3.2 hsa-miR- hsa- 10.510.9 10.2 10.6 0.36 100 10.4 7.98 9.74 9.88 8.96 7.34 9.32 9.06 8.549.03 0.97 100 2.31E−02 1.58 3.0 92b miR30_st1 hsa-asg- hsa-asg- 6.216.04 7.06 6.43 0.54 100 6.30 5.78 4.26 4.26 5.12 5.09 4.54 4.25 3.324.77 0.90 22 1.44E−02 1.66 3.2 8902 8902_st2 hsa-asg- hsa-asg- 5.65 5.595.04 5.43 0.34 67 4.49 3.74 3.91 3.73 3.81 3.69 4.20 3.83 2.92 3.81 0.420 1.47E−04 1.61 3.1 1301 1301_st1 hsa-miR- hsa-miR- 8.10 8.39 8.22 8.240.15 100 6.89 7.22 7.37 6.61 6.11 6.50 6.41 6.62 6.01 6.64 0.46 781.83E−04 1.60 3.0 503 503_st1 hsa-miR- hsa- 7.83 8.47 8.30 8.20 0.33 1007.11 7.33 7.30 6.52 6.57 6.68 6.86 6.89 6.76 6.89 0.30 100 7.52E−05 1.312.5 503 miR141_st1 hsa-asg- hsa-asg- 11.4 11.8 11.6 11.6 0.19 100 10.79.86 10.9 10.8 10.0 9.60 10.2 9.08 9.16 10.0 0.70 100 3.46E−03 1.60 3.0562 562_st1 hsa-asg- hsa-asg- 5.94 6.55 5.91 6.13 0.36 67 3.76 6.13 3.853.73 4.39 5.66 4.07 3.95 5.37 4.55 0.92 0 1.78E−02 1.59 3.0 480814808_st1 hsa-miR- hsa-miR- 6.49 7.01 7.33 6.94 0.43 100 6.08 5.79 4.937.23 6.18 4.55 4.94 3.54 4.95 5.36 1.08 44 3.67E−02 1.59 3.0 189 189_st1hsa-asg- hsa-asg- 7.94 7.95 8.19 8.02 0.14 100 7.43 6.36 6.67 7.04 6.266.27 6.63 6.55 5.09 6.48 0.64 89 2.52E−03 1.55 2.9 9696 9696_st1hsa-asg- hsa-asg- 8.63 8.87 9.47 8.99 0.43 100 7.95 8.62 5.85 7.75 8.678.30 6.83 7.02 6.03 7.45 1.06 78 3.83E−02 1.54 2.9 12356 12356_st1hsa-miR-365 hsa-miR-365_st1 7.11 8.10 7.41 7.54 0.51 100 7.27 5.64 5.537.63 6.38 5.40 6.66 4.78 4.78 6.01 1.03 56 3.66E−02 1.53 2.9 hsa-miR-hsa-asg- 7.18 7.82 7.33 7.44 0.34 100 7.33 5.87 6.09 7.37 6.38 5.46 6.634.78 4.67 6.06 0.98 56 4.27E−02 1.38 2.6 365 13156_st1 hsa-asg-3672hsa-asg-3672_st1 6.50 6.89 6.71 6.70 0.20 100 5.35 6.54 4.17 5.37 5.756.05 4.59 4.49 4.50 5.20 0.81 11 1.17E−02 1.50 2.8 hsa-miR- hsa-miR-6.64 7.61 7.39 7.21 0.50 100 5.80 6.16 5.53 5.92 5.78 3.48 5.82 5.727.23 5.72 0.97 89 3.14E−02 1.50 2.8 424 424_st1 hsa-miR- hsa-miR- 6.976.50 6.70 6.72 0.24 100 5.83 4.16 5.80 6.00 5.70 4.75 5.09 5.32 4.605.25 0.64 33 3.61E−03 1.47 2.8 455 455_st1 hsa-asg- hsa-asg- 5.50 5.485.34 5.44 0.09 33 4.49 3.42 4.87 4.95 4.11 4.18 3.59 2.08 4.07 3.97 0.880 1.88E−02 1.47 2.8 13166 13166_st2 hsa-asg- hsa-asg- 6.86 6.98 5.046.29 1.09 67 5.03 5.37 4.81 4.12 5.22 4.77 5.86 3.99 4.54 4.86 0.60 111.37E−02 1.44 2.7 2213_st2 2213_st2 hsa-asg-9512 hsa-asg-9512_st1 7.017.67 8.37 7.68 0.68 100 6.42 6.94 4.61 6.58 7.81 7.05 6.37 5.29 5.236.25 1.02 11 5.00E−02 1.43 2.7 hsa-asg- hsa-asg- 5.85 6.35 6.40 6.200.31 100 5.18 5.62 4.34 5.21 5.43 5.05 3.50 4.44 4.20 4.78 0.69 07.21E−03 1.43 2.7 3009 3009_st1 hsa-miR- hsa-miR- 5.79 6.77 5.82 6.130.55 67 5.83 4.99 5.24 5.71 4.89 4.46 4.49 3.99 2.74 4.71 0.94 113.59E−02 1.42 2.7 340 340_st1 hsa-miR- hsa-miR- 10.4 10.6 10.6 10.5 0.15100 10.0 8.79 9.44 10.0 9.63 8.27 9.09 8.14 9.14 9.18 0.70 100 7.08E−031.41 2.7 148b 148b_st2 hsa-asg-7215 hsa-asg-7215_st2 6.74 6.67 6.85 6.750.09 100 5.32 6.14 4.81 5.78 5.15 5.76 4.73 5.42 5.00 5.35 0.48 116.10E−04 1.41 2.7 hsa-miR-379 hsa-miR-379_st1 7.81 8.24 7.79 7.95 0.25100 6.52 6.42 7.11 6.97 7.04 6.59 6.35 6.14 6.23 6.60 0.36 100 1.47E−041.35 2.5 hsa-asg- hsa-asg- 5.26 4.03 4.81 4.70 0.62 33 4.09 3.64 4.023.73 2.37 3.37 1.97 3.32 3.77 3.36 0.73 0 1.78E−02 1.34 2.5 31633163_st2 hsa-asg- hsa-asg- 6.13 6.37 6.02 6.17 0.18 100 4.81 4.95 5.895.45 4.97 4.07 5.09 4.21 4.32 4.86 0.59 0 4.42E−03 1.31 2.5 44604460_st2 hsa-asg- hsa-asg- 6.89 7.25 6.87 7.01 0.21 100 5.35 6.10 5.375.51 6.21 5.13 6.14 6.23 5.39 5.72 0.45 56 8.18E−04 1.29 2.4 1190011900_st1 hsa-asg- hsa-asg- 7.24 7.66 7.76 7.55 0.27 100 6.12 5.82 7.486.09 6.14 5.70 6.04 6.93 6.42 6.30 0.57 100 4.82E−03 1.25 2.4 1423714237_st2 hsa-asg- hsa-asg- 5.36 6.06 7.45 6.29 1.07 100 5.32 4.66 5.804.81 4.97 5.17 5.05 4.73 4.84 5.04 0.36 33 8.51E−03 1.25 2.4 41224122_st2 hsa- hsa- 5.33 6.26 5.96 5.85 0.48 100 3.93 5.15 3.91 4.49 5.765.27 4.86 3.83 4.32 4.61 0.69 33 1.74E−02 1.23 2.4 cand443 cand443_st2hsa-asg- hsa-asg- 7.46 7.66 7.49 7.54 0.11 100 6.65 7.38 5.43 6.54 6.826.95 5.54 5.83 5.71 6.32 0.70 56 1.57E−02 1.22 2.3 3728 3728_st1hsa-asg- hsa-asg- 6.51 6.75 6.62 6.63 0.12 100 5.68 4.81 5.71 5.77 5.545.01 5.12 5.29 5.78 5.41 0.36 22 2.47E−04 1.22 2.3 3798 3798_st1hsa-asg-1536 hsa-asg-1536_st2 6.20 6.15 5.04 5.79 0.66 67 5.45 4.66 5.775.17 4.47 3.43 3.85 3.99 4.54 4.59 0.77 11 3.70E−02 1.20 2.3 hsa-asg-67hsa-asg- 4.68 5.34 4.16 4.73 0.59 33 4.55 3.29 4.02 2.33 3.22 3.59 3.683.54 3.65 3.54 0.60 0 1.43E−02 1.19 2.3 67_st2 hsa-asg- hsa-asg- 4.736.11 6.02 5.62 0.77 67 4.02 4.23 4.02 4.86 4.60 4.89 4.20 4.52 4.67 4.450.34 0 3.30E−03 1.18 2.3 12874 12874_st1 hsa-miR- hsa-miR- 8.34 9.138.44 8.64 0.43 100 8.38 8.32 7.31 8.03 8.04 6.51 6.91 6.53 7.20 7.470.74 100 2.98E−02 1.17 2.2 19b 19b_st2 hsa-miR- hsa-miR- 11.9 12.2 12.112.1 0.16 100 11.6 11.2 11.1 11.5 11.1 10.9 10.9 10.0 9.96 10.9 0.58 1007.69E−03 1.16 2.2 30b 30b_st2 hsa-miR-30e-5p hsa-miR-30e-5p_st2 10.611.1 10.4 10.7 0.36 100 10.4 9.64 9.71 10.5 9.81 9.23 9.23 8.15 9.509.58 0.70 100 2.27E−02 1.16 2.2 hsa-asg- hsa-asg- 5.94 5.51 5.34 5.600.31 67 5.29 4.16 4.54 5.12 4.04 4.31 4.20 3.91 4.60 4.46 0.47 113.47E−03 1.13 2.2 11867 11867_st1 hsa-asg- hsa-asg- 4.68 4.87 6.02 5.190.73 33 3.85 4.95 3.67 4.26 4.87 3.48 3.59 4.21 3.65 4.06 0.55 01.67E−02 1.13 2.2 3742 3742_st1 hsa-asg- hsa-asg- 5.79 6.43 6.58 6.270.42 67 5.32 6.01 4.72 5.53 4.45 5.76 4.90 4.71 4.92 5.15 0.53 08.36E−03 1.12 2.2 13047_st2 13047_st2 hsa-asg- hsa-asg- 5.92 4.24 4.494.88 0.91 33 3.21 4.49 4.30 3.01 3.44 4.18 4.14 3.44 3.77 3.77 0.53 02.33E−02 1.11 2.2 4513_st2 4513_st2 hsa-miR- hsa-miR- 7.79 8.22 7.407.80 0.41 100 6.40 7.30 7.22 6.96 6.63 7.66 5.73 5.86 6.60 6.71 0.65 1002.21E−02 1.10 2.1 494 494_st1 hsa-miR- hsa- 7.11 7.72 7.23 7.35 0.32 1006.83 6.55 6.85 6.43 6.38 7.10 5.40 5.79 6.89 6.47 0.55 89 2.78E−02 0.891.8 494 miR61_st2 hsa-asg- hsa-asg- 7.35 7.28 6.52 7.05 0.46 100 5.895.96 6.99 6.57 5.61 5.05 5.05 6.49 6.00 5.96 0.66 78 2.58E−02 1.09 2.112807_st2 12807_st2 hsa-asg- hsa-asg- 4.37 5.87 4.43 4.89 0.85 33 3.933.29 4.21 4.12 4.50 2.99 3.85 3.91 3.39 3.80 0.48 0 1.76E−02 1.09 2.113293_st1 13293_st1 hsa-asg- hsa-asg- 6.36 6.79 6.42 6.53 0.23 100 6.524.30 6.11 5.93 5.24 4.37 5.86 5.62 5.00 5.44 0.77 33 4.14E−02 1.09 2.19719_st1 9719_st1 hsa-miR- hsa-miR- 6.90 6.81 6.33 6.68 0.31 100 6.185.61 6.71 6.01 5.09 4.68 5.71 4.87 5.60 5.61 0.65 44 2.26E−02 1.07 2.118b 18b_st1 hsa-miR- hsa-miR- 12.0 12.3 12.4 12.2 0.25 100 11.6 11.611.2 11.9 11.5 11.6 11.1 10.1 10.02 11.2 0.68 100 2.60E−02 1.07 2.1 30c30c_st1 hsa-asg- hsa-asg- 6.88 6.80 6.60 6.76 0.14 100 6.12 5.40 5.926.19 5.89 5.72 5.40 5.45 5.35 5.72 0.33 33 4.07E−04 1.05 2.1 2500_st12500_st1 hsa-asg- hsa-asg- 5.23 5.28 5.12 5.21 0.08 67 5.25 4.49 3.794.55 4.53 4.18 3.05 3.83 3.87 4.17 0.63 11 1.98E−02 1.04 2.1 7690_st17690_st1 hsa-asg- hsa-asg- 6.57 7.15 6.76 6.83 0.29 100 6.86 5.43 4.756.62 5.93 5.73 5.71 5.77 5.35 5.79 0.64 78 2.49E−02 1.03 2.0 5053_st15053_st1 hsa-miR- hsa-miR- 10.4 10.1 10.5 10.3 0.17 100 10.7 9.36 9.719.95 8.86 8.99 9.01 8.77 8.68 9.35 0.69 100 3.19E−02 1.03 2.0 128a128a_st2 hsa-asg- hsa-asg- 4.40 5.88 4.46 4.91 0.84 33 3.07 3.92 3.915.02 2.97 4.24 3.77 4.31 3.77 3.89 0.62 0 4.50E−02 1.03 2.0 942_st2942_st2 hsa-asg- hsa-asg- 6.00 6.31 5.80 6.03 0.26 67 5.18 5.04 4.615.21 5.15 5.35 4.73 4.68 5.14 5.01 0.27 0 1.73E−04 1.02 2.0 4515_st14515_st1 hsa-asg- hsa-asg- 5.70 5.04 5.23 5.33 0.34 33 4.61 4.23 5.094.46 3.63 4.40 4.20 3.54 4.60 4.31 0.48 0 7.69E−03 1.02 2.0 6883_st16883_st1 hsa-asg-10913_st1 hsa-asg-10913_st1 7.25 7.19 7.23 7.22 0.03100 6.71 5.79 6.77 6.85 5.79 6.20 5.92 5.69 6.18 6.21 0.46 67 4.11E−031.01 2.0 hsa-miR-92 hsa-cand342_st1 13.47 13.62 13.38 13.49 0.12 10013.58 11.97 13.12 12.92 12.28 11.24 12.71 12.42 12.14 12.49 0.69 1003.52E−02 1.01 2.0 hsa-miR-92 hsa-miR-92_st1 13.3 13.5 13.3 13.4 0.14 10013.5 12.0 13.0 12.9 12.2 11.1 12.7 12.4 12.14 12.4 0.68 100 3.97E−020.96 2.0 hsa-asg-12421 hsa-asg-12421_st1 8.47 7.99 8.56 8.34 0.30 1008.10 7.93 6.72 7.33 7.72 7.49 6.61 7.79 6.39 7.34 0.62 44 2.60E−02 1.002.0 SD, standard deviation; %, percentage of samples expressing miRNAabove background levels; Log2Diff, difference in VSN-transformedexpression between LIV and LNneg samples.

Example 23 miRNAs Differentally Expressed Between Normal Ovary Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous ovary cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal ovary tissuethan in cancer-negative lymph nodes.

Total RNA, purified from three separate normal human ovary tissuesamples (FirstChoice® Human Ovary Total RNA, cat. no. AM6974; Ambion,Austin, Tex., USA) and from nine normal human lymph node samples(FirstChoice® Human Lymph Node Total RNA, cat. no. AM7894; Ambion,Austin, Tex., USA) was purchased from Ambion. miRNA purification andlabeling and microarray expression analyses were performed as describedabove in Example 2.

The inventors identified 239 miRNAs (114 verified, 125 predicted) withsignificant differential expression between normal ovary tissue samplesand normal lymph nodes (|log 2Diff| ovary vs LN neg≧1 and p-value<0.05)(Table 22). Among the verified miRNAs, hsa-miR-383 had an averageexpression level over 100-fold higher in ovary samples; hsa-miR-513,-509, and, -493-5p had average expression levels that were more than50-fold higher in ovary samples; 15 miRNAs had average expression levelsthat were between 20- and 50-fold higher in ovary samples; 18 miRNAs hadaverage expression levels that were between 10- and 20-fold higher inovary samples; and 28 miRNAs had average expression levels that werebetween 5- and 10-fold higher in ovary tissue samples than in normallymph nodes. Among the predicted miRNAs, hsa-asg-5638_st2 had an averageexpression level over 100-fold higher in ovary samples;hsa-asg-13404_st2 had an average expression level over 50-fold higher inovary samples; nine miRNAs had average expression levels that werebetween 20- and 50-fold higher in ovary samples; 10 miRNAs had averageexpression levels that were between 10- and 20-fold higher in ovarysamples; and 23 miRNAs had average expression levels that were between5- and 10-fold higher in ovary tissue samples than in normal lymphnodes.

TABLE 22 MicroRNAs Differentially Expressed Between Normal Ovary Samples(OVR) and Cancer-Negative Lymph Node Samples (LNneg) from Patients. OVRLNneg MicroRNA Probe ID 1 2 3 M SD % 1 2 3 4 5 6 7 8 9 M SD % ttestlog2Diff Fold hsa-miR-383 hsa-miR-383_st1 9.65 9.63 8.99 9.42 0.38 1003.33 2.87 2.43 2.60 3.22 1.29 2.64 2.62 1.38 2.49 0.72 0 2.28E−08 −6.94122 hsa-asg- hsa-asg- 10.8 8.98 8.29 9.36 1.30 100 4.02 2.54 2.18 2.601.64 2.61 1.25 2.78 3.25 2.54 0.82 0 6.87E−07 −6.81 112 5638 5638_st2hsa-miR-513 hsa-miR-513_st1 11.9 10.8 10.2 10.99 0.85 100 6.18 6.94 4.985.45 4.04 5.92 0.48 5.15 5.53 4.96 1.87 33 3.55E−04 −6.03 65.3 hsa-asg-hsa-asg- 9.22 8.24 8.93 8.80 0.50 100 4.17 2.71 5.28 2.93 3.28 1.58 2.151.11 2.33 2.84 1.29 0 1.81E−05 −5.96 62.3 13404 13404_st2 hsa-miR-hsa-miR- 12.9 11.6 10.9 11.88 1.00 100 7.11 6.72 5.57 5.80 5.79 6.775.29 5.29 5.42 5.97 0.70 56 4.50E−07 −5.90 59.9 509 509_st2 hsa-miR-hsa-miR- 10.2 10.5 10.6 10.45 0.22 100 4.37 3.83 6.88 6.18 6.33 3.694.38 1.88 4.60 4.68 1.56 33 1.05E−04 −5.77 54.5 493-5p 493-5p_st1hsa-miR-495 hsa-miR-495_st1 10.6 10.9 10.7 10.78 0.16 100 6.02 4.81 6.976.26 6.32 4.43 5.16 2.45 4.89 5.26 1.35 56 4.38E−05 −5.53 46.1hsa-miR202 hsa-miR202_st2 9.36 8.48 8.97 8.94 0.44 100 3.85 2.71 4.933.95 3.63 2.44 4.27 2.45 3.09 3.48 0.87 0 1.27E−06 −5.45 43.9 hsa-miR-hsa-asg- 6.81 7.08 6.49 6.79 0.30 67 0.55 0.54 2.76 1.51 1.37 0.60 1.971.58 1.74 1.40 0.74 0 3.14E−07 −5.39 42.0 493-3p 8480_st1 hsa-miR-hsa-miR- 7.45 7.63 7.00 7.36 0.32 100 4.66 3.10 4.07 3.01 2.28 1.88 4.333.49 4.11 3.43 0.95 0 4.55E−05 −3.92 15.2 493-3p 493-3p_st1 hsa-asg-hsa-asg- 10.1 10.1 9.68 10.00 0.28 100 5.18 4.43 6.99 6.06 5.91 3.544.59 3.07 2.10 4.65 1.57 22 1.99E−04 −5.34 40.6 5021_st1 5021_st1hsa-miR- hsa-miR- 8.36 9.84 9.02 9.07 0.74 100 4.85 3.74 4.54 5.23 5.562.99 3.40 2.27 2.10 3.85 1.27 11 5.83E−05 −5.22 37.3 411 411_st1hsa-miR- hsa-miR- 9.54 10.4 9.77 9.93 0.50 100 5.73 4.49 5.80 6.38 5.992.99 5.05 2.45 3.97 4.76 1.39 44 1.08E−04 −5.17 36.0 299-5p 299-5p_st2hsa-miR- hsa-miR- 8.32 8.45 7.94 8.24 0.27 100 2.78 2.36 4.21 3.40 4.212.99 3.50 1.29 3.25 3.11 0.91 0 3.05E−06 −5.13 34.9 381 381_st2hsa-miR-127 hsa-miR-127_st1 11.7 11.7 11.5 11.66 0.12 100 6.81 5.57 7.957.53 7.69 5.72 6.59 5.49 5.44 6.53 1.02 56 7.52E−06 −5.12 34.8 hsa-asg-hsa-asg- 10.0 10.0 9.71 9.96 0.22 100 5.60 5.37 6.65 5.89 5.90 4.03 4.732.45 3.39 4.89 1.36 56 9.87E−05 −5.07 33.5 13254 13254_st1 hsa-asg-hsa-asg- 9.20 7.74 7.61 8.18 0.88 100 4.24 3.92 2.66 2.77 2.76 4.37 1.082.93 4.24 3.22 1.08 0 3.14E−05 −4.96 31.2 5630 5630_st2 hsa-asg-9696hsa-asg-9696_st1 11.3 11.4 11.5 11.44 0.11 100 7.43 6.36 6.67 7.04 6.266.27 6.63 6.55 5.09 6.48 0.64 89 1.51E−07 −4.96 31.2 hsa-miR- hsa-miR-11.1 11.5 10.9 11.22 0.30 100 6.49 6.52 7.70 8.08 7.70 5.24 5.64 3.995.09 6.27 1.39 67 1.46E−04 −4.95 30.9 368 368_st2 hsa-miR- hsa-miR- 10.510.6 10.3 10.51 0.16 100 6.06 5.84 6.97 6.90 6.25 5.40 4.90 3.74 4.405.61 1.10 67 2.20E−05 −4.90 29.9 487b 487b_st2 hsa-miR- hsa-miR- 9.539.61 10.2 9.80 0.39 100 7.39 1.14 5.89 7.34 4.58 4.18 5.80 4.68 3.774.97 1.94 0 1.96E−03 −4.82 28.3 328 328_st1 hsa-miR- hsa-miR- 9.59 9.719.53 9.61 0.09 100 4.55 5.43 5.92 5.53 5.77 5.68 3.50 4.28 2.74 4.821.13 22 3.23E−05 −4.79 27.6 432 432_st2 hsa-miR- hsa-miR- 9.97 10.0 9.589.85 0.24 100 5.11 5.78 5.99 5.85 5.70 4.21 4.73 4.31 5.02 5.19 0.68 04.79E−07 −4.66 25.3 134 134_st2 hsa-asg-13646 hsa-asg-13646_st1 11.512.0 11.8 11.80 0.23 100 8.24 8.13 6.97 7.98 6.55 7.56 6.68 6.07 7.127.25 0.77 100 1.83E−06 −4.55 23.4 hsa-asg- hsa-asg- 6.66 6.57 6.34 6.520.17 100 1.65 2.16 1.92 2.03 2.90 1.78 3.59 0.93 0.93 1.99 0.85 04.79E−06 −4.53 23.2 7472 7472_st2 hsa-asg-13258 hsa-asg-13258_st2 12.112.6 12.3 12.35 0.23 100 9.05 8.95 7.03 8.66 7.51 8.06 7.19 6.23 7.947.85 0.95 100 1.30E−05 −4.50 22.7 hsa-asg- hsa-asg- 7.11 7.73 7.00 7.280.39 100 3.76 3.29 3.91 3.63 3.44 2.69 1.42 1.48 1.62 2.80 1.03 03.10E−05 −4.48 22.3 5617 5617_st1 hsa-miR- hsa-miR- 7.32 6.95 7.53 7.270.29 100 2.43 2.71 4.54 2.93 4.21 2.35 1.60 1.68 2.92 2.82 1.00 02.47E−05 −4.44 21.8 433 433_st2 hsa-miR- hsa-miR- 10.2 10.8 10.7 10.590.31 100 7.18 5.82 5.98 8.21 6.76 5.46 5.19 5.42 5.78 6.20 0.99 442.49E−05 −4.39 20.9 204 204_st2 hsa-miR- hsa-miR- 10.9 11.2 10.6 10.970.30 100 6.52 6.42 7.11 6.97 7.04 6.59 6.35 6.14 6.23 6.60 0.36 1004.10E−09 −4.37 20.7 379 379_st1 hsa-miR- hsa-miR- 8.52 9.10 8.55 8.720.33 100 5.14 4.08 5.49 5.67 4.85 4.03 2.48 3.83 3.77 4.37 1.01 03.23E−05 −4.35 20.4 376a 376a_st2 hsa-miR-154 hsa-miR-154_st2 7.78 8.437.25 7.82 0.59 100 3.76 2.54 4.30 4.36 4.91 3.13 3.17 1.29 3.97 3.491.10 0 8.28E−05 −4.33 20.1 hsa-miR- hsa-asg- 6.60 7.11 7.56 7.09 0.48100 3.56 2.16 2.43 3.01 2.53 3.13 2.92 2.93 2.74 2.82 0.41 0 3.56E−08−4.27 19.3 450 14228_st2 hsa-miR- hsa-miR- 7.51 7.93 8.60 8.01 0.55 1004.24 2.71 2.86 3.91 3.63 4.03 4.20 4.47 4.07 3.79 0.61 0 9.95E−07 −4.2218.7 450 450_st2 hsa-miR- hsa-miR- 9.49 9.94 10.43 9.96 0.47 100 5.806.16 5.53 5.92 5.78 3.48 5.82 5.72 7.23 5.72 0.97 89 3.26E−05 −4.24 18.9424 424_st1 hsa-miR- hsa-miR- 7.63 7.05 7.35 7.34 0.29 100 3.45 3.293.39 3.68 3.28 1.97 3.50 1.88 3.53 3.11 0.68 0 1.30E−06 −4.24 18.8485-5p 485-5p_st2 hsa-miR-376a hsa-asg-10049_st1 8.86 9.49 8.85 9.070.37 100 5.03 4.91 6.09 5.56 5.35 4.10 3.85 4.14 4.67 4.85 0.74 113.24E−06 −4.21 18.5 hsa-miR- hsa-asg- 7.56 7.92 7.44 7.64 0.25 100 3.334.30 3.39 4.00 4.85 3.69 2.92 4.07 1.86 3.60 0.87 0 1.64E−05 −4.04 16.4376a 14234_st1 hsa-asg- hsa-asg- 6.42 5.43 6.18 6.01 0.52 33 3.45 1.241.79 3.68 2.01 2.85 1.79 0.13 0.63 1.95 1.21 0 2.54E−04 −4.06 16.7 20252025_st2 hsa-miR-299-3p hsa-asg-10378_st1 6.79 7.29 6.38 6.82 0.45 1003.66 3.42 3.23 1.93 2.45 1.97 1.60 3.20 3.53 2.78 0.79 0 8.84E−06 −4.0516.5 hsa-miR- hsa-miR- 6.79 7.17 6.28 6.75 0.45 100 2.43 3.42 3.39 2.933.44 2.77 3.29 1.88 2.92 2.94 0.52 0 5.65E−07 −3.80 14.0 299-3p299-3p_st2 hsa-miR- hsa- 7.16 7.36 7.38 7.30 0.12 100 4.31 2.54 4.123.28 2.45 3.26 3.59 2.78 3.09 3.27 0.65 0 1.15E−06 −4.03 16.3 485-3pmiR23_st2 hsa-miR- hsa-miR- 7.06 7.40 7.12 7.20 0.18 100 3.66 3.02 4.304.43 4.27 3.13 3.68 3.54 3.87 3.77 0.50 0 5.26E−07 −3.43 10.8 485-3p485-3p_st2 hsa-miR- hsa-miR- 6.13 6.84 7.99 6.99 0.94 100 4.31 2.54 2.863.15 1.83 1.78 3.05 2.62 4.54 2.96 0.96 0 8.55E−05 −4.02 16.3 592592_st1 hsa-miR- hsa-asg- 5.63 7.04 7.75 6.81 1.08 100 3.07 3.29 3.671.72 1.64 2.69 3.05 1.68 5.42 2.92 1.21 11 5.92E−04 −3.89 14.8 5924400_st1 hsa-miR- hsa-miR- 8.09 7.40 7.93 7.81 0.36 100 3.93 2.87 3.673.63 3.85 2.85 3.85 4.68 5.18 3.84 0.75 0 6.05E−06 −3.97 15.7 542-5p542-5p_st2 hsa-miR- hsa-miR- 10.9 9.95 9.51 10.14 0.74 100 5.73 5.986.65 6.64 6.19 6.42 5.43 6.13 6.55 6.19 0.42 100 3.51E−07 −3.95 15.4 508508_st1 hsa-asg-14147 hsa-asg-14147_st1 6.69 7.23 7.53 7.15 0.43 33 4.434.33 3.54 3.28 3.10 3.69 3.45 1.68 1.38 3.21 1.05 0 1.07E−04 −3.94 15.3hsa-asg- hsa-asg- 6.13 7.45 7.56 7.05 0.80 100 4.17 3.64 2.86 2.69 3.542.69 3.40 2.78 2.33 3.12 0.59 0 3.29E−06 −3.93 15.2 11443 11443_st1hsa-miR- hsa-miR-539_st1 6.37 6.72 6.31 6.47 0.22 100 4.66 3.10 2.663.40 2.69 2.99 1.97 2.62 1.38 2.55 0.64 0 1.45E−06 −3.92 15.1 hsa-miR-hsa- 5.73 6.10 4.99 5.60 0.56 67 2.05 3.16 1.39 2.42 1.83 1.88 3.50 1.682.10 2.22 0.63 0 9.21E−06 −3.39 10.5 539 miR177_st1 hsa-miR- hsa-miR-11.0 9.86 10.7 10.55 0.62 100 6.89 7.22 7.37 6.61 6.11 6.50 6.41 6.626.01 6.64 0.46 78 3.32E−07 −3.91 15.1 503 503_st1 hsa-miR- hsa- 11.010.0 10.6 10.59 0.53 100 7.11 7.33 7.30 6.52 6.57 6.68 6.86 6.89 6.766.89 0.30 100 2.47E−08 −3.70 13.0 503 miR141_st1 hsa-miR- hsa-miR- 7.257.23 7.33 7.27 0.06 100 3.56 2.71 4.47 4.36 4.36 2.53 2.92 2.62 3.533.45 0.80 0 1.14E−05 −3.82 14.1 369-5p 369-5p_st2 hsa-miR- hsa-asg- 6.607.41 6.91 6.97 0.41 100 3.76 3.42 4.02 2.23 3.72 3.73 3.40 2.78 3.533.40 0.56 0 1.45E−06 −3.58 11.9 369-5p 1521_st1 hsa-miR- hsa-miR- 6.266.08 7.35 6.56 0.69 100 4.61 3.42 2.86 3.15 3.63 2.53 1.60 1.29 2.542.85 1.02 0 1.73E−04 −3.72 13.1 19a 19a_st1 hsa-asg-13166hsa-asg-13166_st2 7.14 8.15 7.69 7.66 0.51 100 4.49 3.42 4.87 4.95 4.114.18 3.59 2.08 4.07 3.97 0.88 0 4.92E−05 −3.68 12.9 hsa-miR-382hsa-miR-382_st1 10.5 10.4 10.1 10.39 0.22 100 7.17 6.85 7.35 7.13 7.436.56 6.29 5.93 5.81 6.72 0.61 78 1.64E−06 −3.67 12.7 hsa- hsa- 5.98 7.125.92 6.34 0.68 100 2.25 1.96 3.79 3.63 2.69 2.17 3.59 1.29 2.74 2.680.86 0 5.56E−05 −3.66 12.7 cand206 cand206_st1 hsa-miR- hsa-miR- 6.545.83 6.20 6.19 0.35 100 2.43 2.36 1.39 3.73 2.53 2.85 3.17 1.88 2.542.54 0.68 0 5.61E−06 −3.64 12.5 410 410_st1 hsa-asg- hsa-asg- 8.31 8.218.55 8.36 0.17 100 5.92 4.49 5.04 6.08 5.09 4.87 4.14 2.93 4.07 4.730.97 22 9.80E−05 −3.62 12.3 8411 8411_st2 Has- hsa- 6.22 7.16 7.67 7.010.73 100 4.61 4.43 2.66 3.40 2.69 2.69 2.78 3.20 4.24 3.41 0.81 04.61E−05 −3.60 12.2 miR144 miR144_st2 hsa-asg- hsa-asg- 10.66 10.8810.66 10.73 0.12 100 7.64 7.18 7.34 8.22 8.03 6.58 6.97 5.74 6.63 7.150.78 89 1.59E−05 −3.58 12.0 10883 10883_st1 hsa-miR- hsa-miR- 6.03 5.885.45 5.79 0.30 100 1.85 2.16 4.12 3.34 2.69 1.01 2.78 2.53 0.21 2.301.18 0 5.93E−04 −3.49 11.2 376b 376b_st1 hsa-miR-101 hsa-miR-101_st18.57 8.98 9.38 8.98 0.40 100 7.18 6.53 4.21 5.98 6.52 4.70 4.86 4.355.09 5.49 1.08 44 3.40E−04 −3.49 11.2 hsa-asg- hsa-asg- 8.74 7.64 7.267.88 0.77 100 4.09 5.64 3.54 4.30 4.39 4.97 3.17 4.14 5.46 4.41 0.82 117.69E−05 −3.47 11.1 11338 11338_st2 hsa-asg- hsa-asg- 7.29 7.23 8.057.52 0.46 100 4.76 4.81 3.91 4.66 3.89 4.24 4.07 3.74 3.53 4.18 0.47 08.20E−07 −3.34 10.2 9687 9687_st1 hsa-miR- hsa-miR- 5.02 5.96 5.87 5.620.52 67 3.45 2.36 2.18 2.33 2.53 2.69 0.76 2.78 2.10 2.35 0.72 03.10E−05 −3.27 9.6 377 377_st1 hsa- hsa- 6.59 7.01 5.82 6.47 0.60 1002.61 4.16 3.67 2.51 3.68 3.69 2.64 2.78 3.25 3.22 0.60 0 1.07E−05 −3.259.5 cand207 cand207_st1 hsa-miR- hsa-miR- 7.44 7.71 7.89 7.68 0.23 1005.25 4.86 2.86 5.10 4.08 4.65 4.49 4.28 4.40 4.44 0.70 0 1.78E−05 −3.249.5 501 501_st1 hsa-miR- hsa-miR- 9.97 10.1 9.94 10.02 0.11 100 7.335.89 7.21 8.24 6.45 5.41 5.19 6.07 9.36 6.79 1.37 78 2.80E−03 −3.22 9.3203 203_st2 hsa-miR-154* hsa-miR-154-_st2 7.35 7.51 7.81 7.56 0.23 1005.83 3.53 4.21 4.99 4.63 3.95 4.27 3.20 4.40 4.33 0.78 11 4.39E−05 −3.229.3 hsa-miR- hsa-miR- 8.04 8.18 8.01 8.08 0.09 100 4.71 5.08 4.39 4.975.05 5.64 3.68 5.48 4.84 4.87 0.58 0 3.37E−06 −3.21 9.2 202 202_st1hsa-miR- hsa-miR- 8.97 8.38 8.90 8.75 0.32 100 5.11 5.30 6.01 6.29 5.745.68 5.40 5.90 4.73 5.57 0.49 78 1.12E−06 −3.18 9.0 542-3p 542-3p_st1hsa-miR- hsa-miR- 8.47 7.93 7.62 8.00 0.43 100 5.29 5.08 4.75 4.66 4.745.09 4.54 4.28 5.09 4.84 0.32 11 8.37E−08 −3.17 9.0 510 510_st2 hsa-asg-hsa-asg- 8.58 8.87 8.47 8.64 0.21 100 6.06 5.72 5.28 6.77 6.26 5.43 5.464.21 4.16 5.48 0.87 44 1.28E−04 −3.15 8.9 14176 14176_st1 hsa-miR-hsa-miR- 11.5 11.2 11.5 11.47 0.17 100 9.72 8.56 8.33 8.35 8.59 8.088.02 8.44 6.88 8.33 0.74 89 3.21E−05 −3.14 8.8 132 132_st1 hsa-asg-hsa-asg- 5.63 5.60 5.54 5.59 0.04 100 1.45 2.54 3.23 1.82 2.37 2.44 3.293.07 2.10 2.48 0.63 0 9.26E−06 −3.11 8.7 14216 14216_st1 hsa-miR-149hsa-miR-149_st2 5.07 6.73 6.64 6.15 0.93 67 4.49 3.87 1.79 4.15 2.761.48 2.48 3.38 3.17 3.07 1.03 0 1.04E−03 −3.08 8.5 hsa-asg-14230hsa-asg-14230_st1 7.04 6.95 6.68 6.89 0.19 100 4.31 3.74 4.39 4.58 3.333.48 2.78 3.95 4.07 3.85 0.57 0 5.18E−06 −3.04 8.2 hsa-asg-10202hsa-asg-10202_st2 5.95 6.15 6.30 6.13 0.17 67 4.71 2.06 3.31 5.25 3.101.97 2.48 3.38 1.74 3.11 1.22 0 2.00E−03 −3.02 8.1 hsa-miR- hsa-miR-9.83 9.56 9.74 9.71 0.14 100 6.40 7.30 7.22 6.96 6.63 7.66 5.73 5.866.60 6.71 0.65 100 1.59E−05 −3.00 8.0 494 494_st1 hsa-miR- hsa- 8.908.70 8.58 8.73 0.16 100 6.83 6.55 6.85 6.43 6.38 7.10 5.40 5.79 6.896.47 0.55 89 5.02E−05 −2.26 4.8 494 miR61_st2 hsa-miR- hsa-miR- 11.311.5 11.7 11.53 0.20 100 9.29 8.99 7.93 9.15 8.63 8.44 7.79 7.91 8.618.53 0.56 100 4.53E−06 −3.00 8.0 660 660_st1 hsa-miR- hsa-miR- 8.40 8.178.53 8.37 0.18 100 6.27 5.73 6.17 5.72 5.17 5.11 4.59 4.98 4.76 5.390.61 11 1.01E−05 −2.98 7.9 212 212_st2 hsa-asg- hsa-asg- 9.31 9.71 9.179.39 0.28 100 6.62 6.49 6.49 7.56 7.45 5.68 6.35 5.00 6.23 6.43 0.79 671.05E−04 −2.96 7.8 13304 13304_st2 hsa-asg- hsa-asg- 8.77 9.43 8.47 8.890.49 100 6.42 6.07 5.71 6.89 6.85 4.84 5.33 5.29 6.16 5.95 0.71 06.65E−05 −2.94 7.7 279 279_st2 hsa-miR-487a hsa-miR-487a_st2 5.38 5.835.12 5.44 0.36 33 2.25 2.87 2.66 3.40 2.19 3.26 0.91 2.27 2.92 2.53 0.750 8.09E−05 −2.91 7.5 hsa-miR- hsa-asg- 6.01 6.23 5.80 6.01 0.21 100 4.093.64 3.91 3.40 3.89 3.78 3.68 3.74 3.65 3.75 0.20 0 1.15E−08 −2.26 4.8487a 6915_st1 hsa-miR-9* hsa-miR-9-_st2 8.71 8.87 7.74 8.44 0.61 1006.20 6.10 5.57 6.11 5.41 5.87 4.94 4.73 5.23 5.57 0.54 44 1.47E−05 −2.877.3 hsa-asg- hsa-asg-13127_st2 9.08 9.39 9.53 9.33 0.23 100 7.75 7.365.86 6.68 6.05 6.84 5.66 5.08 7.13 6.49 0.88 67 3.14E−04 −2.84 7.2 13127hsa-asg- hsa-asg- 9.09 9.42 9.51 9.34 0.22 100 7.77 7.24 6.28 7.01 5.926.62 4.98 5.81 6.89 6.50 0.85 89 2.42E−04 −2.84 7.1 922 922_st1hsa-miR-214 hsa-miR-214_st1 12.3 12.4 12.5 12.45 0.12 100 9.76 9.55 9.7610.9 10.3 9.93 9.27 8.44 8.52 9.62 0.81 100 1.65E−04 −2.83 7.1 hsa-miR-hsa- 11.5 11.6 12.3 11.83 0.46 100 10.4 7.98 9.74 9.88 8.96 7.34 9.329.06 8.54 9.03 0.97 100 8.41E−04 −2.80 7.0 92b miR30_st1 hsa-miR-hsa-miR- 11.9 11.9 12.5 12.15 0.33 100 10.9 8.50 10.1 10.2 9.25 7.889.75 9.23 8.88 9.42 0.94 100 7.25E−04 −2.73 6.7 92b 92b_st1 hsa-miR-hsa-miR- 5.66 5.94 5.97 5.86 0.17 100 3.76 3.29 3.06 3.15 3.85 2.17 2.643.32 2.54 3.09 0.56 0 8.97E−06 −2.77 6.8 502 502_st1 hsa-asg- hsa-asg-5.73 6.12 5.97 5.94 0.20 100 4.17 2.71 2.66 3.68 3.44 2.44 3.05 2.274.16 3.17 0.72 0 7.98E−05 −2.76 6.8 9695 9695_st2 hsa-asg- hsa-asg- 6.767.02 6.92 6.90 0.13 100 4.85 4.30 4.98 4.76 3.72 2.85 4.07 3.32 4.544.16 0.73 0 9.46E−05 −2.75 6.7 9681 9681_st1 hsa-asg- hsa-asg- 6.52 6.826.56 6.63 0.16 100 4.90 2.06 3.60 5.83 4.85 2.85 4.73 3.78 2.44 3.891.28 0 4.98E−03 −2.74 6.7 13284 13284_st1 hsa-asg- hsa-asg- 6.35 5.404.75 5.50 0.80 33 1.25 2.63 5.30 4.63 1.83 2.53 2.92 1.39 2.44 2.77 1.380 9.68E−03 −2.73 6.6 14065 14065_st2 hsa-asg- hsa-asg- 5.38 5.34 5.575.43 0.13 33 2.93 2.36 2.66 3.52 3.72 2.53 2.78 1.48 2.54 2.72 0.65 04.17E−05 −2.71 6.5 14174 14174_st1 Has- hsa- 10.7 11.1 11.2 11.06 0.25100 8.90 8.31 8.71 8.69 8.14 7.82 7.95 8.41 8.42 8.37 0.36 100 3.47E−07−2.69 6.4 miR102 miR102_st2 hsa-miR- hsa-miR- 8.00 7.40 8.33 7.91 0.47100 5.83 4.16 5.80 6.00 5.70 4.75 5.09 5.32 4.60 5.25 0.64 33 6.73E−05−2.66 6.3 455 455_st1 hsa-asg- hsa-asg-8878_st2 11.8 11.6 11.9 11.830.18 100 10.9 9.01 9.13 11.2 9.75 8.53 10.2 8.11 5.85 9.20 1.64 1002.26E−02 −2.63 6.2 8878 hsa-miR-345 hsa-miR-345_st2 8.67 9.37 8.86 8.970.36 100 6.73 7.38 6.31 6.47 6.67 7.02 5.12 5.34 6.15 6.35 0.74 781.75E−04 −2.61 6.1 hsa-asg- hsa-asg- 9.16 9.52 9.41 9.36 0.18 100 8.067.48 5.53 8.01 7.35 7.37 6.68 5.32 5.00 6.75 1.18 78 4.18E−03 −2.61 6.14557 4557_st2 hsa-miR- hsa-miR- 9.85 10.0 10.2 10.07 0.22 100 8.38 8.327.31 8.03 8.04 6.51 6.91 6.53 7.20 7.47 0.74 100 1.69E−04 −2.60 6.1 19b19b_st2 hsa-asg- hsa-asg- 6.60 6.42 6.33 6.45 0.14 100 3.85 3.02 4.874.66 4.00 4.18 2.48 4.21 3.39 3.85 0.77 0 2.12E−04 −2.60 6.1 33763376_st1 hsa-asg- hsa-asg- 6.92 5.89 5.85 6.22 0.61 100 5.07 3.53 4.474.15 3.39 1.19 3.05 4.07 3.97 3.65 1.10 0 3.65E−03 −2.57 5.9 1420814208_st1 hsa-miR- hsa-miR- 10.6 11.1 11.1 10.98 0.27 100 8.75 8.43 8.788.81 8.19 7.84 8.13 8.43 8.51 8.43 0.33 100 2.96E−07 −2.56 5.9 500500_st1 hsa-asg- hsa-asg- 5.77 6.15 6.96 6.30 0.61 100 4.90 2.87 4.024.26 3.93 2.69 3.68 3.44 4.24 3.78 0.70 0 2.50E−04 −2.52 5.7 1296412964_st2 hsa-miR- hsa-miR- 11.5 11.5 11.7 11.62 0.14 100 10.5 9.57 9.779.80 8.72 9.01 9.09 7.97 7.54 9.11 0.94 100 1.17E−03 −2.51 5.7 423423_st1 hsa-asg- hsa-asg- 6.82 7.13 7.04 7.00 0.16 100 5.62 5.68 2.965.39 5.57 4.03 3.59 2.70 4.81 4.48 1.19 11 5.40E−03 −2.51 5.7 1039110391_st2 hsa-miR- hsa-miR- 7.83 8.11 7.61 7.85 0.25 100 6.08 5.79 4.937.23 6.18 4.55 4.94 3.54 4.95 5.36 1.08 44 3.29E−03 −2.49 5.6 189189_st1 hsa-asg- hsa-asg- 5.51 5.80 6.48 5.93 0.50 67 4.90 3.16 3.914.63 3.68 2.99 3.29 2.93 1.62 3.46 0.98 0 2.16E−03 −2.47 5.5 17011701_st2 hsa-miR-329 hsa-miR-329_st1 5.73 6.31 5.57 5.87 0.39 100 3.763.02 4.61 3.28 4.58 2.85 2.78 3.32 2.74 3.44 0.73 0 2.99E−04 −2.43 5.4hsa-miR- hsa-asg- 5.25 5.58 5.69 5.51 0.23 100 1.65 3.16 3.79 3.73 4.422.35 3.68 2.78 3.09 3.18 0.84 0 9.98E−04 −2.32 5.0 329 13589_st2hsa-miR- hsa-asg- 5.49 5.83 6.10 5.81 0.30 67 4.09 2.87 4.21 3.40 4.243.48 4.00 2.62 3.53 3.61 0.59 0 1.16E−04 −2.20 4.6 329 13204_st2hsa-miR- hsa-asg- 8.38 8.64 8.62 8.55 0.15 100 7.75 7.03 6.53 7.45 6.625.05 5.71 3.83 5.23 6.13 1.28 67 1.01E−02 −2.41 5.3 140 14239_st1hsa-miR- hsa-miR- 7.99 8.60 8.22 8.27 0.31 100 7.73 6.16 6.37 7.22 6.635.50 4.78 4.78 5.27 6.05 1.05 44 5.48E−03 −2.23 4.7 140 140_st2hsa-miR-9 hsa-miR-9_st2 5.02 6.55 5.51 5.70 0.78 67 3.45 3.53 2.86 3.283.63 2.17 3.50 3.32 4.16 3.32 0.55 0 1.51E−04 −2.37 5.2 hsa-miR-9hsa-cand90_st2 3.52 5.78 4.75 4.68 1.13 33 3.56 3.16 2.66 3.01 2.83 2.172.64 1.68 3.65 2.82 0.63 0 4.12E−03 −1.87 3.6 hsa-asg- hsa-asg- 6.825.66 5.66 6.05 0.67 33 3.21 3.78 5.12 3.08 2.69 2.53 3.29 4.76 4.70 3.680.96 0 2.90E−03 −2.37 5.2 3036 3036_st1 hsa-miR- hsa-miR- 11.49 11.9411.76 11.73 0.23 100 10.20 9.76 9.14 9.80 9.38 9.47 9.04 8.51 9.05 9.370.50 100 1.75E−05 −2.36 5.1 151 151_st2 hsa-miR- hsa-miR- 9.86 7.74 7.998.53 1.16 100 5.78 5.58 6.77 6.13 6.26 6.02 6.38 6.35 6.44 6.19 0.36 1001.84E−04 −2.34 5.1 506 506_st2 hsa-miR-574 hsa-miR-574_st1 10.7 11.711.6 11.36 0.55 100 9.35 8.42 9.39 10.2 9.96 8.12 9.20 8.06 8.47 9.020.80 100 8.93E−04 −2.34 5.1 hsa-asg- hsa-asg- 7.27 7.92 7.42 7.54 0.34100 5.62 4.76 5.71 5.53 5.68 5.20 4.78 5.19 4.73 5.24 0.41 22 5.87E−06−2.29 4.9 5827 5827_st2 hsa-miR- hsa-miR- 12.6 12.8 12.9 12.82 0.12 10011.0 10.5 10.5 11.4 11.1 10.1 10.1 9.52 10.3 10.5 0.59 100 7.55E−05−2.28 4.9 99b 99b_st1 hsa-miR- hsa-asg- 12.7 12.9 12.9 12.89 0.14 10011.2 10.6 10.6 11.6 11.3 10.3 10.3 9.55 10.5 10.7 0.65 100 2.19E−04−2.19 4.6 99b 14196_st2 hsa-miR- hsa-miR- 9.32 9.61 8.82 9.25 0.40 1007.43 7.60 6.23 8.75 7.86 6.49 6.51 5.62 6.69 7.02 0.96 33 3.53E−03 −2.234.7 497 497_st1 hsa-miR- hsa- 10.3 10.5 9.58 10.16 0.52 100 8.20 8.567.51 9.37 8.88 7.78 7.45 7.13 7.83 8.08 0.74 89 1.18E−03 −2.08 4.2 497miR43_st1 hsa-miR- hsa- 12.0 12.4 12.5 12.35 0.25 100 10.4 9.85 9.7210.9 10.8 9.26 10.0 9.56 10.6 10.1 0.59 100 1.06E−04 −2.22 4.7 130acand144_st1 hsa-miR-130a hsa-miR-130a_st1 12.0 12.3 12.4 12.27 0.20 10010.3 9.96 9.71 10.9 10.7 9.21 10.0 9.63 10.4 10.1 0.56 100 8.75E−05−2.15 4.4 hsa-miR- hsa- 6.65 7.50 7.12 7.09 0.42 100 5.45 4.81 5.37 6.664.11 4.43 4.82 4.58 3.87 4.90 0.84 22 1.71E−03 −2.19 4.6 499 miR12_st1hsa-miR- hsa-miR- 6.54 7.32 6.76 6.87 0.40 100 5.32 4.66 4.75 6.58 4.534.18 5.05 4.28 4.60 4.88 0.73 22 1.30E−03 −1.99 4.0 499 499_st1 hsa-miR-hsa-miR- 9.28 9.64 9.59 9.51 0.19 100 7.43 7.71 7.93 7.61 7.47 7.14 6.946.57 7.06 7.32 0.42 100 7.51E−06 −2.19 4.6 362 362_st1 hsa-asg- hsa-asg-6.64 7.25 7.29 7.06 0.37 100 4.66 4.37 4.02 6.18 5.09 4.21 5.16 5.225.05 4.88 0.66 11 3.19E−04 −2.18 4.5 7465 7465_st2 hsa-miR-550hsa-miR-550-2_st1 4.84 5.78 5.40 5.34 0.47 33 3.76 3.53 5.09 4.79 2.611.19 1.97 2.45 3.09 3.16 1.27 0 1.83E−02 −2.17 4.5 hsa-asg- hsa-asg-7.97 8.38 8.42 8.25 0.25 100 5.92 6.06 6.67 6.74 6.18 6.02 6.12 4.586.44 6.08 0.63 78 2.13E−04 −2.17 4.5 11883 11883_st1 hsa-miR- hsa-miR-12.6 12.6 12.9 12.76 0.13 100 12.1 11.1 11.5 10.5 10.2 11.0 10.2 9.189.38 10.6 0.96 100 3.85E−03 −2.16 4.5 148a 148a_st1 hsa-asg- hsa-asg-6.65 7.96 8.10 7.57 0.80 100 5.68 5.19 5.28 6.40 6.25 5.59 4.98 4.475.35 5.46 0.60 33 6.38E−04 −2.11 4.3 3145 3145_st1 hsa-miR- hsa-miR-9.95 9.89 10.5 10.12 0.34 100 9.76 7.76 8.97 9.69 7.39 7.36 7.88 7.636.14 8.06 1.19 89 1.67E−02 −2.06 4.2 505 505_st1 hsa-asg- hsa-asg- 5.665.80 5.38 5.62 0.21 100 4.81 3.74 3.39 3.46 4.42 3.99 3.40 1.68 3.393.59 0.87 0 3.15E−03 −2.03 4.1 11749 11749_st1 hsa- hsa- 4.92 6.04 5.765.57 0.58 33 3.66 3.96 3.96 4.43 3.49 3.19 1.60 3.87 3.92 3.57 0.81 02.97E−03 −2.01 4.0 cand863 cand863_st2 hsa-miR- hsa- 5.63 5.71 5.20 5.510.27 67 3.93 2.54 1.92 5.43 5.30 2.53 3.17 3.20 3.65 3.52 1.21 02.09E−02 −1.99 4.0 193a cand178_st1 hsa-asg- hsa-asg- 8.39 8.52 9.218.71 0.44 100 6.79 6.98 7.19 7.29 6.98 6.52 6.22 6.32 6.27 6.73 0.41 223.16E−05 −1.98 3.9 12346 12346_st2 hsa-miR-222 hsa-miR-222_st2 11.7 11.411.9 11.70 0.26 100 10.4 9.56 9.54 10.2 9.75 9.33 9.52 9.38 9.86 9.730.37 100 7.45E−06 −1.97 3.9 hsa-asg- hsa-asg- 7.75 8.21 8.38 8.11 0.32100 7.46 6.04 7.10 7.36 4.72 5.57 6.22 7.09 3.77 6.15 1.28 78 2.83E−02−1.97 3.9 13230 13230_st2 hsa-miR- hsa-miR- 6.92 6.61 6.90 6.81 0.18 1004.66 5.28 4.72 5.06 4.72 5.40 4.33 4.49 5.12 4.86 0.37 0 5.79E−06 −1.953.9 370 370_st1 hsa-miR- hsa- 4.75 5.52 5.20 5.16 0.39 33 3.45 2.54 3.674.15 3.72 2.85 3.59 1.68 3.25 3.21 0.75 0 1.78E−03 −1.94 3.8 135bcand78_st1 hsa-miR- hsa-miR- 12.7 12.6 13.1 12.84 0.25 100 11.2 11.111.2 11.4 11.4 10.8 10.4 10.1 10.0 10.9 0.55 100 1.85E−04 −1.94 3.8 320320_st1 hsa-miR- hsa-miR- 7.19 7.99 7.17 7.45 0.47 100 6.66 5.97 5.216.67 5.29 5.13 5.02 4.25 5.44 5.51 0.79 33 2.78E−03 −1.94 3.8 339339_st1 hsa-asg- hsa-asg- 5.90 6.08 6.20 6.06 0.15 33 5.18 3.96 5.014.58 4.42 4.10 2.32 4.25 3.46 4.14 0.86 0 3.99E−03 −1.92 3.8 58295829_st1 hsa-asg- hsa-asg- 13.3 13.4 13.5 13.48 0.10 100 12.2 11.9 11.212.2 12.0 11.4 11.3 10.4 11.4 11.6 0.58 100 3.05E−04 −1.88 3.7 1119911199_st2 hsa-miR-641 hsa-miR-641_st1 4.41 5.94 4.58 4.98 0.84 33 4.092.87 3.79 2.77 2.19 3.54 3.68 2.62 2.33 3.10 0.69 0 2.95E−03 −1.88 3.7hsa-miR- hsa-asg- 7.26 8.14 8.24 7.88 0.54 100 6.61 5.82 6.85 6.61 5.635.68 5.69 5.81 5.53 6.02 0.51 67 3.07E−04 −1.85 3.6 181d 13508_st1hsa-miR- hsa-miR- 9.68 10.2 10.4 10.11 0.38 100 9.13 7.83 8.91 8.93 7.597.96 8.28 8.21 8.02 8.32 0.54 100 3.86E−04 −1.80 3.5 181d 181d_st1hsa-miR- hsa- 9.38 9.90 10.2 9.84 0.43 100 8.97 8.22 8.99 8.80 8.02 8.258.10 8.17 7.88 8.38 0.42 100 4.30E−04 −1.46 2.8 181d miR74_st1hsa-miR-28 hsa-miR- 12.0 12.3 12.5 12.30 0.25 100 11.1 10.7 10.0 11.010.8 10.1 10.1 9.40 10.5 10.4 0.57 100 3.39E−04 −1.85 3.6 28_st1hsa-miR- hsa- 6.13 6.90 7.00 6.68 0.48 100 5.94 5.23 4.02 6.58 5.46 4.245.64 3.07 3.53 4.86 1.19 44 3.04E−02 −1.82 3.5 365 cand708_st1 hsa-asg-hsa-asg- 6.72 7.05 6.66 6.81 0.21 100 4.71 6.29 6.06 5.88 5.25 5.56 4.442.27 4.47 4.99 1.23 11 3.29E−02 −1.82 3.5 2924 2924_st1 hsa-miR-hsa-miR- 10.7 10.8 11.4 11.03 0.37 100 10.0 9.44 9.16 9.78 9.72 7.508.94 8.77 9.53 9.21 0.76 100 3.05E−03 −1.82 3.5 29c 29c_st2 hsa-asg-hsa-asg- 5.49 5.73 6.14 5.79 0.33 100 5.03 3.74 4.30 4.99 4.65 2.53 3.403.32 3.87 3.98 0.84 0 5.33E−03 −1.81 3.5 11756 11756_st1 hsa-miR-197hsa-miR-197_st1 10.6 11.0 11.1 10.93 0.29 100 10.2 8.61 9.99 9.95 9.078.06 9.21 8.52 8.59 9.13 0.76 100 3.08E−03 −1.80 3.5 hsa-miR- hsa-miR-10.8 11.2 11.3 11.14 0.28 100 10.7 9.36 9.71 9.95 8.86 8.99 9.01 8.778.68 9.35 0.69 100 1.61E−03 −1.80 3.5 128a 128a_st2 hsa-miR- hsa-miR-7.91 7.05 7.34 7.43 0.43 100 5.14 5.04 6.67 6.09 6.11 5.17 5.92 5.455.27 5.65 0.57 44 6.00E−04 −1.78 3.4 409-5p 409-5p_st1 hsa-miR- hsa-miR-11.3 11.5 11.6 11.51 0.16 100 11.2 9.83 10.0 10.3 9.19 9.33 9.54 9.199.25 9.77 0.69 100 1.86E−03 −1.74 3.3 128b 128b_st1 hsa-asg- hsa-asg-7.40 8.27 7.24 7.64 0.55 100 6.16 6.49 6.21 6.31 4.24 7.42 5.73 4.835.75 5.90 0.93 78 1.36E−02 −1.73 3.3 1519 1519_st1 hsa-asg- hsa-asg-6.91 6.63 6.81 6.78 0.14 100 5.48 5.06 4.96 6.07 5.36 4.52 5.05 3.785.20 5.05 0.64 11 1.08E−03 −1.73 3.3 5715 5715_st2 hsa-asg- hsa-asg-5.05 5.68 6.17 5.63 0.56 67 4.24 3.64 4.12 4.26 4.33 3.95 3.50 3.74 3.393.91 0.35 0 7.72E−05 −1.73 3.3 6543 6543_st1 hsa-asg- hsa-asg- 4.81 4.895.38 5.03 0.31 33 4.43 2.87 3.91 4.00 3.54 2.85 3.05 2.62 2.54 3.31 0.680 2.04E−03 −1.72 3.3 7745 7745_st2 hsa-miR- hsa-asg- 6.60 5.85 6.43 6.290.39 100 3.21 6.25 5.09 4.52 4.45 5.20 4.59 4.21 3.65 4.58 0.89 111.03E−02 −1.72 3.3 654 33_st1 hsa-asg- hsa-asg- 6.52 5.68 6.68 6.29 0.54100 5.45 4.66 5.77 5.17 4.47 3.43 3.85 3.99 4.54 4.59 0.77 11 5.78E−03−1.70 3.2 1536 1536_st2 hsa-asg- hsa-asg- 7.77 8.03 8.60 8.13 0.42 1007.09 6.49 6.38 6.53 6.50 6.52 6.07 6.50 5.93 6.45 0.33 100 2.70E−05−1.69 3.2 4637 4637_st1 hsa-asg- hsa-asg- 5.76 4.71 4.39 4.95 0.71 332.78 3.42 3.23 2.69 3.89 3.95 3.29 3.32 2.92 3.28 0.44 0 5.90E−04 −1.683.2 8486 8486_st2 Has- hsa- 9.71 10.2 10.6 10.19 0.46 100 9.47 8.16 9.729.39 8.42 7.95 8.50 7.61 7.41 8.51 0.84 100 9.12E−03 −1.67 3.2 miR157miR157_st2 hsa-asg- hsa-asg- 5.30 5.40 5.20 5.30 0.10 67 4.71 4.16 4.543.63 3.44 3.59 3.40 2.27 2.92 3.63 0.77 0 4.54E−03 −1.67 3.2 22992299_st1 hsa-asg- hsa-asg- 7.23 8.10 7.99 7.77 0.48 100 5.70 7.71 5.435.08 6.59 7.81 5.16 6.08 5.71 6.14 1.03 44 2.64E−02 −1.63 3.1 49124912_st2 hsa-asg- hsa-asg- 5.53 5.92 6.08 5.84 0.28 100 5.32 4.16 4.124.49 4.00 3.87 4.14 3.44 4.32 4.21 0.51 11 4.30E−04 −1.63 3.1 1390513905_st1 hsa-miR- hsa-asg- 7.84 8.15 8.27 8.09 0.22 100 7.53 5.85 7.377.77 6.85 4.77 6.64 5.46 5.93 6.47 1.02 56 2.46E−02 −1.62 3.1 33114205_st1 hsa-miR- hsa-miR- 9.34 9.56 9.52 9.48 0.12 100 9.04 7.57 8.358.79 7.69 7.09 7.70 7.28 7.20 7.86 0.71 100 3.34E−03 −1.62 3.1 331331_st2 hsa-asg- hsa-asg- 5.79 5.68 6.12 5.86 0.23 100 4.61 5.08 4.304.55 5.12 4.55 3.29 2.45 4.32 4.25 0.86 11 1.10E−02 −1.61 3.1 522522_st1 hsa-miR- hsa-miR- 11.9 12.1 12.3 12.12 0.20 100 11.2 10.4 10.910.8 9.83 10.3 10.2 10.4 10.1 10.5 0.44 100 1.35E−04 −1.61 3.0 181b181b_st2 hsa-miR- hsa-miR- 2.27 4.80 5.87 4.31 1.85 33 2.61 2.36 3.391.93 2.37 3.37 2.15 3.32 2.92 2.71 0.56 0 3.25E−02 −1.60 3.0 137 137_st1hsa-miR-92 hsa- 14.0 13.9 14.2 14.07 0.17 100 13.5 11.9 13.1 12.9 12.211.2 12.7 12.4 12.1 12.4 0.69 100 3.33E−03 −1.59 3.0 cand342_st1hsa-miR-92 hsa-miR- 13.9 13.8 14.1 13.98 0.12 100 13.5 12.0 13.0 12.912.2 11.1 12.7 12.4 12.1 12.4 0.68 100 3.98E−03 −1.52 2.9 92_st1hsa-asg- hsa-asg- 6.69 6.66 6.11 6.49 0.33 67 5.78 4.58 5.35 5.50 4.915.32 4.14 3.95 4.57 4.90 0.63 0 2.21E−03 −1.59 3.0 14138 14138_st2hsa-asg- hsa-asg- 7.25 7.39 7.13 7.26 0.13 100 5.60 5.72 5.67 6.74 5.705.30 5.25 5.00 6.09 5.68 0.51 56 4.37E−04 −1.58 3.0 7471 7471_st1hsa-asg- hsa-asg- 6.95 6.78 7.29 7.01 0.26 100 6.52 4.30 6.11 5.93 5.244.37 5.86 5.62 5.00 5.44 0.77 33 7.07E−03 −1.57 3.0 9719 9719_st1hsa-let-7e hsa-let- 13.9 13.7 13.9 13.85 0.13 100 12.6 12.8 12.3 12.612.3 12.6 12.0 11.3 11.8 12.2 0.48 100 3.17E−04 −1.56 2.9 7e_st1hsa-asg- hsa-asg- 6.44 6.10 6.35 6.30 0.18 100 6.30 5.78 4.26 4.26 5.125.09 4.54 4.25 3.32 4.77 0.90 22 1.82E−02 −1.53 2.9 8902 8902_st2hsa-asg- hsa-asg- 8.15 8.03 8.66 8.28 0.34 100 7.26 7.04 6.40 7.06 7.116.98 6.41 6.29 6.21 6.75 0.41 89 1.92E−04 −1.53 2.9 6951 6951_st2hsa-asg- hsa-asg- 6.48 5.85 6.33 6.22 0.33 100 5.18 4.86 4.54 4.81 5.954.43 3.93 4.35 4.32 4.71 0.59 22 2.04E−03 −1.51 2.9 2967 2967_st2hsa-asg- hsa-asg- 7.74 7.16 7.72 7.54 0.33 100 6.89 6.98 5.06 5.95 6.497.01 4.94 6.34 4.57 6.03 0.95 67 2.49E−02 −1.51 2.8 6277 6277_st2hsa-asg- hsa-asg- 11.2 11.6 11.7 11.55 0.26 100 10.7 9.86 10.9 10.8 10.09.60 10.2 9.08 9.16 10.0 0.70 100 5.46E−03 −1.50 2.8 562 562_st1hsa-asg- hsa-asg- 5.84 6.12 5.87 5.94 0.15 100 4.90 4.08 4.75 5.12 5.014.31 3.59 4.14 4.24 4.46 0.51 0 6.89E−04 −1.48 2.8 1565 1565_st2hsa-asg- hsa-asg- 8.12 7.85 8.52 8.16 0.33 100 7.33 7.11 5.45 7.16 6.786.86 6.78 6.84 5.84 6.68 0.63 33 3.39E−03 −1.48 2.8 872 872_st2 hsa-asg-hsa-asg- 5.02 4.98 5.35 5.12 0.20 33 4.37 3.53 4.39 3.40 4.11 2.85 2.643.83 3.65 3.64 0.62 0 2.69E−03 −1.48 2.8 2286 2286_st2 hsa-asg- hsa-asg-6.01 5.76 5.94 5.90 0.13 33 5.11 5.32 5.30 5.02 5.43 4.55 3.59 2.70 2.834.43 1.10 0 4.80E−02 −1.47 2.8 14015 14015_st1 hsa-asg- hsa-asg- 9.207.89 8.90 8.66 0.68 100 7.86 8.02 6.90 6.99 7.42 7.88 6.22 7.07 6.367.19 0.66 89 7.48E−03 −1.47 2.8 8872 8872_st1 hsa-asg- hsa-asg- 6.926.75 7.43 7.03 0.35 100 5.68 5.25 6.12 6.30 5.59 5.60 5.35 5.06 5.205.57 0.42 11 3.12E−04 −1.46 2.8 11181 11181_st1 hsa-miR- hsa-miR- 13.113.3 13.4 13.32 0.12 100 12.2 11.8 11.9 12.6 12.2 11.4 11.7 10.9 11.711.8 0.50 100 7.12E−04 −1.44 2.7 99a 99a_st2 hsa-miR- hsa- 13.0 13.213.2 13.16 0.08 100 12.5 11.7 12.2 12.8 12.4 11.5 11.8 11.1 11.6 11.90.56 100 5.64E−03 −1.16 2.2 99a cand160_st2 hsa-asg- hsa-asg- 5.28 6.176.46 5.97 0.62 67 5.70 4.91 4.54 5.28 4.39 4.97 4.44 2.78 3.77 4.53 0.8611 2.51E−02 −1.44 2.7 1177 1177_st2 hsa-asg- hsa-asg- 5.07 5.83 4.525.14 0.66 33 4.17 2.87 3.39 4.55 4.39 2.69 4.14 2.93 4.32 3.72 0.74 01.46E−02 −1.42 2.7 6758 6758_st1 hsa-miR- hsa-miR- 7.86 7.86 7.58 7.770.16 100 6.98 5.77 6.79 7.12 6.21 4.65 6.35 6.03 7.18 6.34 0.81 891.49E−02 −1.42 2.7 17-3p 17-3p_st1 hsa-asg- hsa-asg- 6.91 7.06 7.30 7.090.20 100 5.85 5.84 5.45 5.90 5.92 6.00 5.40 5.15 5.63 5.68 0.29 441.61E−05 −1.41 2.7 3038 3038_st2 hsa-miR- hsa-miR- 10.6 10.5 9.84 10.350.44 100 8.04 9.33 9.47 9.54 8.91 9.18 8.03 8.82 9.17 8.94 0.57 1003.04E−03 −1.41 2.7 34a 34a_st1 hsa-asg- hsa-asg- 5.30 5.78 5.31 5.460.27 33 4.61 3.42 4.87 4.49 3.54 2.99 4.82 4.68 3.09 4.06 0.78 01.38E−02 −1.41 2.6 12840 12840_st1 hsa-asg- hsa-asg- 5.63 5.87 5.90 5.800.15 100 5.57 4.16 4.93 4.91 4.42 2.99 5.05 3.32 4.32 4.41 0.83 111.89E−02 −1.39 2.6 1766 1766_st2 hsa-asg- hsa-asg- 6.24 6.06 6.34 6.210.14 33 5.60 5.83 4.58 4.66 5.39 4.91 3.68 4.85 3.92 4.82 0.72 09.11E−03 −1.39 2.6 2315 2315_st2 hsa-asg- hsa-asg- 6.01 6.06 6.33 6.130.17 100 5.73 5.33 4.39 5.27 5.29 4.75 4.73 3.83 3.53 4.76 0.74 331.11E−02 −1.37 2.6 7319 7319_st2 hsa-miR- hsa-miR- 10.8 11.2 11.0 11.030.19 100 10.8 9.87 10.1 10.0 9.58 8.72 9.33 8.80 9.65 9.66 0.66 1006.36E−03 −1.37 2.6 192 192_st2 hsa- hsa- 7.97 6.96 7.46 7.47 0.51 1006.75 7.27 5.91 5.65 6.24 6.29 5.05 6.14 5.58 6.10 0.66 56 8.67E−03 −1.372.6 cand173 cand173_st1 hsa-asg- hsa-asg- 5.71 5.06 5.50 5.42 0.33 335.22 3.29 4.54 4.40 4.27 2.69 4.00 3.83 4.32 4.06 0.73 0 1.27E−02 −1.362.6 5545 5545_st2 hsa-miR- hsa-miR- 10.6 11.0 11.0 10.92 0.22 100 10.49.64 9.71 10.5 9.81 9.23 9.23 8.15 9.50 9.58 0.70 100 9.87E−03 −1.34 2.530e-5p 30e-5p_st2 hsa-asg- hsa-asg- 5.76 5.60 4.25 5.20 0.83 67 4.173.16 3.91 4.08 4.89 2.69 3.05 4.41 4.40 3.86 0.73 0 2.34E−02 −1.34 2.512240 12240_st1 hsa-asg- hsa-asg- 5.38 5.76 4.85 5.33 0.45 33 4.17 3.423.91 5.56 4.58 4.18 2.78 2.93 4.54 4.01 0.87 0 3.36E−02 −1.32 2.5 83808380_st2 hsa-miR- hsa-miR- 12.9 13.1 13.2 13.12 0.12 100 12.5 12.3 11.212.3 12.1 12.2 11.7 10.7 10.8 11.8 0.70 100 1.02E−02 −1.32 2.5 10b10b_st2 hsa-miR- hsa-miR- 10.8 10.6 10.8 10.79 0.09 100 9.91 9.64 10.010.0 9.43 8.17 9.21 9.10 9.76 9.48 0.59 100 4.14E−03 −1.31 2.5 106b106b_st2 hsa-miR- hsa-miR- 12.6 12.8 12.8 12.77 0.09 100 11.7 11.6 11.212.0 11.7 11.7 11.1 10.9 11.1 11.4 0.37 100 1.76E−04 −1.29 2.4 361361_st2 hsa-miR- hsa-miR- 13.4 13.4 13.6 13.50 0.11 100 13.1 12.2 12.213.1 12.6 11.8 11.7 11.1 11.9 12.2 0.65 100 8.12E−03 −1.28 2.4 100100_st2 hsa-miR- hsa- 13.0 13.2 13.2 13.19 0.11 100 13.0 12.1 12.3 12.812.5 11.7 11.6 11.2 11.9 12.1 0.58 100 1.46E−02 −1.02 2.0 100cand375_st1 hsa- hsa- 9.71 10.3 10.2 10.11 0.36 100 9.62 9.10 9.23 9.558.87 8.54 8.50 8.15 7.91 8.83 0.60 100 6.33E−03 −1.28 2.4 miR257miR257_st1 hsa-asg- hsa-asg- 6.84 7.38 8.30 7.51 0.73 100 6.32 7.09 6.036.08 6.14 6.40 5.69 6.03 6.41 6.24 0.39 78 2.69E−03 −1.26 2.4 1193811938_st1 hsa-miR- hsa-miR- 7.49 8.04 7.07 7.53 0.48 100 6.59 6.67 6.426.87 6.43 6.02 5.86 6.26 5.29 6.27 0.48 89 2.88E−03 −1.26 2.4 425-3p425_st2 hsa-asg- hsa-asg- 6.44 6.62 6.95 6.67 0.26 100 5.48 5.82 5.416.09 5.69 4.75 5.22 4.83 5.42 5.41 0.44 44 9.16E−04 −1.26 2.4 64626462_st1 hsa-asg- hsa-asg- 9.84 9.93 10.0 9.94 0.11 100 9.39 10.1 8.178.70 8.96 9.66 7.51 7.93 7.66 8.69 0.94 44 4.83E−02 −1.26 2.4 85428542_st2 hsa-asg- hsa-asg- 6.61 6.31 6.23 6.38 0.20 100 5.73 5.79 4.815.32 5.53 5.17 4.64 4.28 4.89 5.13 0.51 22 2.44E−03 −1.25 2.4 57405740_st2 hsa-miR- hsa-miR- 13.4 13.5 13.6 13.54 0.07 100 12.5 12.3 12.013.2 12.8 11.9 11.9 11.0 12.5 12.2 0.64 100 8.37E−03 −1.25 2.4 27b27b_st1 hsa-miR- hsa-miR- 13.0 13.1 13.3 13.18 0.14 100 12.7 11.4 12.212.7 12.4 10.8 12.0 11.3 11.5 11.9 0.68 100 1.21E−02 −1.25 2.4 125a125a_st2 hsa-asg- hsa-asg- 11.1 9.89 10.7 10.60 0.66 100 10.1 10.1 8.979.23 9.89 9.97 8.64 9.33 7.88 9.36 0.78 89 3.40E−02 −1.24 2.4 90929092_st2 hsa-miR- hsa-miR- 6.48 7.07 6.50 6.68 0.34 100 4.76 5.12 6.416.24 5.05 5.24 4.73 5.11 6.41 5.45 0.70 56 1.62E−02 −1.23 2.4 34b34b_st2 hsa-asg- hsa-asg- 6.17 6.02 6.28 6.16 0.13 100 6.00 5.37 4.615.17 5.62 5.35 5.16 3.64 3.39 4.92 0.88 11 4.15E−02 −1.23 2.3 62876287_st2 hsa-asg- hsa-asg- 7.67 7.40 7.61 7.56 0.14 100 7.36 6.37 6.196.97 6.35 5.27 5.82 5.74 6.88 6.33 0.66 89 1.14E−02 −1.23 2.3 925925_st1 hsa-asg- hsa-asg- 5.34 5.87 5.24 5.48 0.34 67 3.85 4.08 3.915.75 4.91 4.24 4.73 3.07 3.77 4.26 0.78 11 2.75E−02 −1.23 2.3 25962596_st2 hsa-miR- hsa-miR- 12.4 12.4 12.4 12.42 0.02 100 12.0 11.7 10.912.2 11.6 11.1 10.8 9.75 10.5 11.2 0.79 100 2.70E−02 −1.22 2.3 152152_st1 hsa-asg- hsa-asg- 5.76 6.00 6.08 5.94 0.17 100 5.75 4.30 4.475.34 5.12 3.95 4.94 4.47 4.24 4.73 0.59 11 6.55E−03 −1.21 2.3 1008110081_st1 hsa-asg- hsa-asg- 7.42 6.91 7.45 7.26 0.30 0 5.60 6.42 6.436.33 5.78 6.22 6.76 5.42 5.63 6.06 0.47 11 2.17E−03 −1.20 2.3 84768476_st1 hsa-asg- hsa-asg- 5.76 5.96 5.85 5.85 0.10 100 5.70 4.30 5.675.43 4.11 3.59 4.27 4.28 4.84 4.69 0.76 22 2.79E−02 −1.17 2.2 1046510465_st2 hsa-asg- hsa-asg- 7.28 7.97 8.44 7.90 0.58 100 7.02 6.32 6.727.46 6.95 6.64 6.46 6.23 6.78 6.73 0.38 100 2.26E−03 −1.16 2.2 23012301_st2 hsa-miR- hsa-miR- 13.2 13.3 13.4 13.32 0.09 100 12.8 12.1 12.412.7 12.4 11.6 11.9 11.3 11.9 12.1 0.49 100 2.78E−03 −1.15 2.2 125b125b_st1 hsa-miR- hsa-miR- 7.51 7.47 7.09 7.36 0.23 100 7.35 6.80 6.017.48 6.36 5.50 5.73 5.42 5.23 6.21 0.84 56 4.61E−02 −1.15 2.2 489489_st2 hsa-asg- hsa-asg- 5.30 5.13 4.52 4.98 0.41 33 4.99 3.02 3.674.95 4.04 3.59 3.05 3.32 3.87 3.83 0.73 0 2.88E−02 −1.15 2.2 187 187_st1hsa-asg- hsa-asg- 10.5 10.4 10.6 10.57 0.09 100 10.5 9.46 9.58 9.81 9.178.63 9.51 8.96 9.11 9.43 0.56 100 6.85E−03 −1.14 2.2 13308 13308_st2hsa-miR-628 hsa-miR-628_st2 8.56 8.85 8.85 8.75 0.17 100 8.30 7.88 7.208.00 7.35 7.52 7.24 7.66 7.44 7.62 0.37 100 5.65E−04 −1.13 2.2 hsa-asg-hsa-asg- 5.53 5.95 5.92 5.80 0.24 100 5.32 5.04 5.04 4.93 4.42 3.87 3.934.44 5.09 4.67 0.53 33 6.03E−03 −1.12 2.2 317 317_st2 hsa-miR- hsa-miR-7.43 6.75 6.89 7.02 0.36 100 6.73 6.17 5.12 5.83 6.14 6.15 5.56 6.045.44 5.91 0.48 56 4.51E−03 −1.11 2.2 566 566_st1 hsa-asg- hsa-asg- 5.845.46 5.85 5.72 0.22 67 4.31 4.71 4.87 4.81 4.30 4.65 4.73 4.28 4.78 4.610.24 0 3.49E−05 −1.11 2.2 6770_st1 6770_st1 hsa-asg-10401hsa-asg-10401_st1 5.60 5.34 5.48 5.47 0.13 67 5.11 5.04 3.91 5.04 4.244.55 4.20 4.07 3.09 4.36 0.65 22 1.78E−02 −1.11 2.2 hsa-asg- hsa-asg-7.53 7.20 7.51 7.41 0.18 100 6.96 7.18 5.74 6.27 6.67 6.52 5.54 6.575.35 6.31 0.64 67 1.70E−02 −1.10 2.1 5326 5326_st2 hsa-asg-4448hsa-asg-4448_st1 7.20 6.97 6.94 7.04 0.15 100 6.73 6.14 5.98 7.05 6.175.01 6.26 4.87 5.31 5.95 0.75 67 3.50E−02 −1.09 2.1 hsa-asg- hsa-asg-6.06 5.98 5.82 5.95 0.12 100 4.81 4.95 5.89 5.45 4.97 4.07 5.09 4.214.32 4.86 0.59 0 1.21E−02 −1.09 2.1 4460 4460_st2 hsa-asg- hsa-asg- 5.934.98 5.28 5.39 0.49 33 4.61 4.23 5.09 4.46 3.63 4.40 4.20 3.54 4.60 4.310.48 0 7.24E−03 −1.09 2.1 6883 6883_st1 hsa-miR- hsa-miR- 9.76 10.3 10.010.03 0.28 100 9.83 9.37 8.91 9.35 8.95 8.34 8.55 8.21 9.05 8.95 0.52100 7.50E−03 −1.08 2.1 194 194_st2 hsa-asg- hsa-asg- 7.93 8.46 8.61 8.330.35 100 7.49 7.02 7.05 8.02 7.65 7.30 7.27 6.50 7.03 7.26 0.43 1003.26E−03 −1.07 2.1 3967 3967_st2 hsa-asg- hsa-asg- 5.45 5.71 5.94 5.700.25 100 5.22 4.66 4.39 5.17 5.35 4.73 4.07 3.99 4.32 4.66 0.50 06.97E−03 −1.05 2.1 3124 3124_st2 hsa-asg-12179 hsa-asg-12179_st1 6.456.26 6.38 6.36 0.10 100 6.16 5.04 5.45 5.68 5.39 4.93 4.82 5.04 5.355.32 0.42 11 2.04E−03 −1.05 2.1 hsa-asg- hsa-asg- 7.31 7.56 8.08 7.650.39 100 7.03 7.28 5.83 6.74 6.74 6.49 5.90 6.52 6.91 6.61 0.48 897.37E−03 −1.04 2.1 12325 12325_st2 hsa-asg- hsa-asg- 5.90 6.45 6.66 6.340.39 100 5.07 5.55 6.14 5.75 5.05 5.57 4.54 5.26 4.78 5.30 0.50 568.86E−03 −1.04 2.1 3854 3854_st1 hsa-asg- hsa-asg- 4.87 4.44 5.66 4.990.62 33 3.93 4.49 4.21 4.55 3.89 4.43 4.38 3.20 2.54 3.96 0.68 04.34E−02 −1.03 2.0 8519 8519_st2 hsa-asg-10922 hsa-asg-10922_st2 6.836.83 7.06 6.91 0.14 100 6.55 5.79 5.98 5.86 6.23 5.52 5.82 5.77 5.425.88 0.34 56 6.20E−04 −1.03 2.0 hsa-asg- hsa-asg- 7.88 8.78 8.13 8.260.47 100 7.58 7.47 7.55 8.17 7.24 6.59 6.87 7.00 6.68 7.24 0.51 1001.16E−02 −1.03 2.0 13279 13279_st1 hsa-asg- hsa-asg- 9.53 9.23 9.50 9.420.17 100 8.99 8.81 8.09 8.96 8.79 8.43 8.04 7.94 7.51 8.40 0.53 1009.05E−03 −1.02 2.0 954 954_st1 hsa-asg-7688 hsa-asg-7688_st1 7.00 7.137.25 7.13 0.13 100 6.69 6.16 6.47 6.38 5.38 6.15 6.11 5.86 5.81 6.110.39 78 1.60E−03 −1.02 2.0 SD, standard deviation; %, percentage ofsamples expressing miRNA above background levels; Log2Diff, differencein VSN-transformed expression between OVR and LNneg samples.

Example 24 miRNAs Differentally Expressed Between Normal Prostate Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous prostate cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal prostatetissue than in cancer-negative lymph nodes.

Total RNA, purified from three separate normal human prostate tissuesamples (FirstChoice® Human Prostate Total RNA, cat. no. AM7988; Ambion,Austin, Tex., USA) and from nine normal human lymph node samples(FirstChoice® Human Lymph Node Total RNA, cat. no. AM7894; Ambion,Austin, Tex., USA) was purchased from Ambion. miRNA purification andlabeling and microarray expression analyses were performed as describedabove in Example 2.

The inventors identified 159 miRNAs (72 verified, 87 predicted) withsignificant differential expression between normal prostate and normallymph nodes samples (|log 2Diff| prostate vs LN neg≧1 and p-value<0.05)(Table 23). Among the verified miRNAs, hsa-miR-205 and -375 had averageexpression levels over 100-fold higher in prostate tissue samples;hsa-miR-429, -206 and -187 had average expression levels over 50-foldhigher in prostate samples; 13 miRNAs (hsa-miR-200b, -200a, -133b,-200c, -133a, -203, -493-5p, -605, -487b, -141, -96, -495, and -149) hadaverage expression levels that were between 10- and 40-fold higher inprostate samples; and 18 miRNAs had average expression levels that werebetween 5- and 10-fold higher in prostate samples than in normal lymphnodes. Among the predicted miRNAs, hsa-asg-14153_st2 had an averageexpression level that was over 30-fold higher in prostate samples;hsa-asg-7637_st2, -4915_st1 and 5021_st1) had average expression levelsthat were between 10- and 20-fold higher in prostate samples; and 28miRNAs had average expression levels that were between 5- and 10-foldhigher in prostate tissue samples than in normal lymph nodes.

TABLE 23 MicroRNAs Differentially Expressed Between Normal ProstateSamples (PRO) and Cancer-Negative Lymph Node Samples (LNneg) fromPatients. PRO LNneg Micro RNA Probe ID 1 2 3 M SD % 1 2 3 4 5 6 7 8 9 MSD % ttest log2 Diff Fold hsa-miR-205 hsa-miR-205_st1 11.7 12.6 13.412.6 0.84 100 2.93 5.91 5.64 2.03 3.59 6.11 3.40 5.32 7.76 4.74 1.85 333.90E−05 −7.86 232 hsa-miR-375 hsa-miR-375_st1 12.0 10.5 12.9 11.8 1.18100 5.96 4.93 6.23 2.85 2.97 3.69 0.99 4.63 6.39 4.29 1.82 0 5.83E−05−7.57 190 hsa-miR-429 hsa-miR-429_st1 7.99 9.26 9.52 8.92 0.82 100 3.451.54 3.67 2.13 2.37 2.69 2.32 2.45 5.39 2.89 1.14 0 7.94E−06 −6.03 65.5hsa-miR-206 hsa-miR-206_st2 10.2 12.0 11.7 11.3 0.94 100 4.09 4.99 1.929.39 3.97 5.85 8.46 4.47 5.00 5.35 2.31 44 1.59E−03 −6.01 64.6hsa-miR-187 hsa-miR-187_st1 7.99 9.53 9.69 9.07 0.94 100 4.76 2.79 2.763.77 3.22 3.37 3.05 3.38 3.17 3.37 0.61 0 2.05E−07 −5.70 52.2hsa-miR-200b hsa-miR-200b_st1 12.7 12.6 13.3 12.8 0.36 100 7.62 7.808.90 6.69 6.82 6.87 6.51 7.65 10.4 7.70 1.26 100 4.63E−05 −5.19 36.5hsa-asg-14153 hsa-asg-14153_st2 7.68 7.13 8.76 7.86 0.83 67 3.07 2.263.06 3.01 2.37 2.69 1.16 2.78 4.40 2.75 0.86 0 4.22E−06 −5.10 34.4hsa-miR-200a hsa-miR-200a_st2 10.0 10.5 11.1 10.5 0.55 100 6.27 5.086.49 5.72 5.29 4.60 4.07 4.78 7.78 5.56 1.14 67 2.96E−05 −5.02 32.5hsa-miR-133b hsa-miR-133b_st2 10.5 11.3 11.6 11.1 0.55 100 7.36 6.224.47 10.1 7.45 6.20 7.22 4.47 5.39 6.55 1.78 67 1.58E−03 −4.62 24.6hsa-miR-200c hsa-miR-200c_st1 12.9 13.3 14.0 13.4 0.51 100 9.60 8.8410.3 8.18 8.15 8.14 7.98 8.89 11.5 9.07 1.21 100 1.37E−04 −4.40 21.1hsa-asg-7637 hsa-asg-7637_st2 7.82 7.31 9.13 8.09 0.94 100 3.66 2.544.47 3.46 3.93 4.03 3.29 3.64 5.27 3.81 0.76 0 1.19E−05 −4.28 19.4hsa-miR-133a hsa-miR-133a_st1 11.2 11.9 12.3 11.8 0.53 100 7.77 7.705.92 10.8 8.19 7.14 8.22 6.25 6.48 7.61 1.47 89 7.28E−04 −4.26 19.1hsa-miR-203 hsa-miR-203_st2 9.22 11.0 11.6 10.6 1.26 100 7.33 5.89 7.218.24 6.45 5.41 5.19 6.07 9.36 6.79 1.37 78 1.65E−03 −3.84 14.3hsa-asg-4915 hsa-asg-4915_st1 5.75 6.65 8.02 6.80 1.14 100 2.43 3.832.18 2.60 3.63 2.92 2.78 2.08 4.47 2.99 0.81 0 7.52E−05 −3.81 14.0hsa-miR-493- hsa-miR-493- 8.12 8.58 8.73 8.48 0.32 100 4.37 3.83 6.886.18 6.33 3.69 4.38 1.88 4.60 4.68 1.56 33 2.32E−03 −3.80 13.9 5p 5p_st1hsa-miR-605 hsa-miR-605_st1 6.10 5.87 6.85 6.27 0.51 100 2.61 2.16 3.060.75 1.74 2.17 2.92 3.07 4.47 2.55 1.03 0 1.58E−04 −3.73 13.2hsa-miR-487b hsa-miR-487b_st2 8.61 9.09 10.0 9.24 0.71 100 6.06 5.846.97 6.90 6.25 5.40 4.90 3.74 4.40 5.61 1.10 67 3.65E−04 −3.63 12.4hsa-miR-141 hsa-miR-141_st1 6.41 8.42 8.49 7.77 1.18 100 5.07 3.83 4.934.43 4.39 2.85 3.85 3.83 4.54 4.19 0.68 0 5.60E−05 −3.58 12.0 hsa-miR-96hsa-miR-96_st1 6.90 6.98 6.55 6.81 0.23 100 4.99 3.42 3.06 3.01 4.112.85 3.05 1.88 3.09 3.27 0.86 11 4.73E−05 −3.54 11.6 hsa-miR-495hsa-miR-495_st1 8.33 8.64 9.40 8.79 0.55 100 6.02 4.81 6.97 6.26 6.324.43 5.16 2.45 4.89 5.26 1.35 56 1.54E−03 −3.53 11.6 hsa-asg-5021hsa-asg-5021_st1 7.61 7.99 8.89 8.16 0.66 100 5.18 4.43 6.99 6.06 5.913.54 4.59 3.07 2.10 4.65 1.57 22 4.28E−03 −3.51 11.4 hsa-miR-149hsa-miR-149_st2 6.03 6.08 7.52 6.55 0.84 67 4.49 3.87 1.79 4.15 2.761.48 2.48 3.38 3.17 3.07 1.03 0 3.87E−04 −3.48 11.2 hsa-miR-432hsa-miR-432_st2 7.51 8.04 8.85 8.13 0.67 100 4.55 5.43 5.92 5.53 5.775.68 3.50 4.28 2.74 4.82 1.13 22 8.16E−04 −3.31 9.9 hsa-miR-189hsa-miR-189_st1 7.73 9.11 9.12 8.65 0.80 100 6.08 5.79 4.93 7.23 6.184.55 4.94 3.54 4.95 5.36 1.08 44 7.36E−04 −3.30 9.8 hsa-miR-582hsa-miR-582_st1 7.21 7.74 8.31 7.75 0.55 100 5.85 2.16 4.54 5.96 4.894.10 4.07 4.35 4.67 4.51 1.12 22 8.02E−04 −3.24 9.5 hsa-asg-13254hsa-asg-13254_st1 7.48 8.05 8.79 8.11 0.66 100 5.60 5.37 6.65 5.89 5.904.03 4.73 2.45 3.39 4.89 1.36 56 3.20E−03 −3.22 9.3 hsa-miR-200a*hsa-miR-200a-_st2 5.48 5.64 6.57 5.90 0.59 100 3.07 2.87 2.18 3.91 2.611.78 2.15 3.20 2.92 2.74 0.65 0 2.20E−05 −3.15 8.9 hsa-miR-539hsa-miR-539_st1 4.74 5.81 6.44 5.66 0.86 67 2.05 3.16 2.66 3.40 2.692.99 1.97 2.62 1.38 2.55 0.64 0 4.97E−05 −3.12 8.7 hsa-miR-539hsa-miR177_st1 4.39 5.62 5.64 5.21 0.72 67 2.61 2.54 1.39 2.42 1.83 1.883.50 1.68 2.10 2.22 0.63 0 4.05E−05 −3.00 8.0 hsa-asg-924hsa-asg-924_st1 5.28 6.62 7.64 6.51 1.18 67 4.66 4.30 3.39 4.46 2.692.77 2.15 2.78 3.53 3.41 0.89 0 6.72E−04 −3.10 8.6 hsa-miR-411hsa-miR-411_st1 5.70 7.53 7.55 6.93 1.07 100 4.85 3.74 4.54 5.23 5.562.99 3.40 2.27 2.10 3.85 1.27 11 3.77E−03 −3.07 8.4 hsa-asg-11088hsa-asg-11088_st1 5.39 5.57 5.17 5.38 0.20 67 1.25 1.05 3.85 1.82 0.942.35 1.60 3.38 4.50 2.31 1.31 0 2.83E−03 −3.07 8.4 hsa-asg-10131hsa-asg-10131_st2 5.54 6.48 6.83 6.29 0.67 100 3.33 3.16 3.54 2.51 2.533.26 2.64 3.20 5.09 3.25 0.78 0 1.37E−04 −3.04 8.2 hsa-miR-409-3phsa-miR-409-3p_st1 5.58 6.95 7.85 6.79 1.14 33 2.25 3.47 5.14 4.99 4.772.99 3.96 2.62 3.77 3.77 1.05 0 1.68E−03 −3.02 8.1 hsa-asg-11715hsa-asg-11715_st2 4.20 4.75 5.85 4.93 0.84 33 1.85 2.36 2.18 1.51 2.111.78 0.62 1.48 3.39 1.92 0.75 0 1.61E−04 −3.01 8.1 hsa-asg-10247hsa-asg-10247_st1 4.59 5.71 6.31 5.54 0.87 67 2.78 2.16 4.02 1.72 2.192.35 0.35 3.20 3.97 2.53 1.14 0 2.07E−03 −3.01 8.1 hsa-asg-13404hsa-asg-13404_st2 5.22 4.90 7.38 5.84 1.35 33 4.17 2.71 5.28 2.93 3.281.58 2.15 1.11 2.33 2.84 1.29 0 6.15E−03 −3.00 8.0 hsa-miR-204hsa-miR-204_st2 8.86 8.36 10.24 9.15 0.98 100 7.18 5.82 5.98 8.21 6.765.46 5.19 5.42 5.78 6.20 0.99 44 1.16E−03 −2.96 7.8 hsa-miR-381hsa-miR-381_st2 4.77 7.74 5.68 6.06 1.52 67 2.78 2.36 4.21 3.40 4.212.99 3.50 1.29 3.25 3.11 0.91 0 1.93E−03 −2.95 7.7 hsa-miR-183hsa-miR-183_st1 9.91 9.59 10.13 9.88 0.27 100 7.66 6.76 6.71 7.93 7.565.83 7.52 6.32 6.65 6.99 0.70 89 5.14E−05 −2.88 7.4 hsa-miR-154hsa-miR-154_st2 5.83 7.13 6.14 6.37 0.68 100 3.76 2.54 4.30 4.36 4.913.13 3.17 1.29 3.97 3.49 1.10 0 1.88E−03 −2.87 7.3 hsa-asg-5617hsa-asg-5617_st1 5.40 5.79 5.84 5.68 0.24 100 3.76 3.29 3.91 3.63 3.442.69 1.42 1.48 1.62 2.80 1.03 0 9.29E−04 −2.87 7.3 hsa-asg-5119hsa-asg-5119_st1 5.08 5.29 5.81 5.39 0.38 100 2.43 2.71 1.92 1.93 2.012.53 2.92 2.62 3.65 2.53 0.55 0 9.36E−06 −2.87 7.3 hsa-asg-4978hsa-asg-4978_st2 5.26 5.04 5.84 5.38 0.41 100 1.65 2.87 2.86 1.72 2.011.97 2.78 2.62 4.16 2.52 0.79 0 1.50E−04 −2.86 7.3 hsa-asg-12139hsa-asg-12139_st2 5.56 5.37 4.69 5.21 0.46 67 2.78 2.36 2.66 2.33 1.642.17 1.60 2.08 3.53 2.35 0.59 0 1.98E−05 −2.86 7.3 hsa-asg-1296hsa-asg-1296_st1 6.40 5.29 6.59 6.09 0.70 100 3.07 2.54 4.81 2.42 2.532.77 3.17 3.91 4.16 3.27 0.84 0 4.01E−04 −2.83 7.1 hsa-asg-13705hsa-asg-13705_st1 4.99 4.79 5.50 5.09 0.37 67 1.65 0.95 3.79 2.33 2.692.35 0.35 3.20 3.25 2.29 1.12 0 2.04E−03 −2.81 7.0 hsa-asg-14176hsa-asg-14176_st1 7.32 8.69 8.66 8.23 0.78 100 6.06 5.72 5.28 6.77 6.265.43 5.46 4.21 4.16 5.48 0.87 44 7.11E−04 −2.74 6.7 hsa-miR-368hsa-miR-368_st2 7.82 9.82 9.38 9.01 1.05 100 6.49 6.52 7.70 8.08 7.705.24 5.64 3.99 5.09 6.27 1.39 67 1.16E−02 −2.73 6.7 hsa-miR-329hsa-miR-329_st1 5.93 6.28 6.13 6.12 0.18 100 3.76 3.02 4.61 3.28 4.582.85 2.78 3.32 2.74 3.44 0.73 0 1.14E−04 −2.68 6.4 hsa-miR-329hsa-asg-13589_st2 5.52 5.85 5.53 5.63 0.19 100 1.65 3.16 3.79 3.73 4.422.35 3.68 2.78 3.09 3.18 0.84 0 6.70E−04 −2.45 5.5 hsa-miR-329hsa-asg-13204_st2 5.94 6.12 6.09 6.05 0.09 100 4.09 2.87 4.21 3.40 4.243.48 4.00 2.62 3.53 3.61 0.59 0 3.79E−05 −2.45 5.4 hsa-asg-13166hsa-asg-13166_st2 6.12 6.67 7.10 6.63 0.49 100 4.49 3.42 4.87 4.95 4.114.18 3.59 2.08 4.07 3.97 0.88 0 6.32E−04 −2.66 6.3 hsa-miR-499hsa-miR12_st1 6.16 8.55 7.91 7.54 1.24 100 5.45 4.81 5.37 6.66 4.11 4.434.82 4.58 3.87 4.90 0.84 22 1.72E−03 −2.64 6.2 hsa-miR-499hsa-miR-499_st1 5.90 8.15 7.92 7.32 1.24 100 5.32 4.66 4.75 6.58 4.534.18 5.05 4.28 4.60 4.88 0.73 22 1.61E−03 −2.44 5.4 hsa-miR-127hsa-miR-127_st1 8.83 8.69 9.84 9.12 0.63 100 6.81 5.57 7.95 7.53 7.695.72 6.59 5.49 5.44 6.53 1.02 56 2.25E−03 −2.59 6.0 hsa-miR-376ahsa-miR-376a_st2 6.10 7.78 6.98 6.95 0.84 100 5.14 4.08 5.49 5.67 4.854.03 2.48 3.83 3.77 4.37 1.01 0 2.72E−03 −2.58 6.0 hsa-miR-376ahsa-asg-14234_st1 4.74 7.08 6.60 6.14 1.24 67 3.33 4.30 3.39 4.00 4.853.69 2.92 4.07 1.86 3.60 0.87 0 2.57E−03 −2.54 5.8 hsa-miR-376ahsa-asg-10049_st1 6.16 8.16 7.42 7.25 1.01 100 5.03 4.91 6.09 5.56 5.354.10 3.85 4.14 4.67 4.85 0.74 11 1.20E−03 −2.39 5.2 hsa-asg-10055hsa-asg-10055_st1 4.55 4.43 4.64 4.54 0.11 33 2.05 0.95 2.18 1.11 2.012.69 1.42 2.27 2.92 1.96 0.68 0 7.92E−05 −2.58 6.0 hsa-asg-10587hsa-asg-10587_st2 4.47 5.14 6.36 5.32 0.96 67 3.07 3.02 3.06 3.34 1.833.32 1.60 2.62 2.92 2.75 0.63 0 2.77E−04 −2.57 5.9 hsa-asg-4219hsa-asg-4219_st1 4.96 4.83 5.22 5.00 0.20 67 3.45 1.75 1.66 1.51 2.281.78 2.92 2.78 3.97 2.46 0.87 0 6.70E−04 −2.55 5.8 hsa-asg-11160hsa-asg-11160_st2 5.41 5.96 5.88 5.75 0.30 100 2.78 3.16 3.39 3.08 2.902.69 2.48 3.54 5.14 3.24 0.79 0 3.69E−04 −2.51 5.7 hsa-miR-410hsa-miR-410_st1 4.30 5.81 5.01 5.04 0.76 67 2.43 2.36 1.39 3.73 2.532.85 3.17 1.88 2.54 2.54 0.68 0 3.11E−04 −2.50 5.6 hsa-asg-1088hsa-asg-1088_st1 4.22 3.47 5.64 4.44 1.10 33 1.85 2.16 1.66 1.61 2.110.84 3.29 1.48 2.54 1.95 0.69 0 8.13E−04 −2.50 5.6 hsa-asg-10106hsa-asg-10106_st1 3.53 4.94 5.65 4.71 1.08 33 3.33 2.36 1.66 1.31 2.451.78 1.97 2.62 2.54 2.22 0.61 0 4.54E−04 −2.48 5.6 hsa-asg-10080hsa-asg-10080_st1 3.99 5.32 5.13 4.81 0.72 33 2.05 1.75 2.43 1.82 2.372.44 2.48 2.27 3.39 2.33 0.48 0 4.12E−05 −2.48 5.6 hsa-asg-3822hsa-asg-3822_st2 4.22 5.57 5.86 5.22 0.87 67 2.05 3.02 3.67 2.03 2.452.17 1.60 2.93 4.78 2.75 0.99 0 3.29E−03 −2.47 5.6 hsa-miR-369-5phsa-asg-1521_st1 5.33 6.13 6.15 5.87 0.47 67 3.76 3.42 4.02 2.23 3.723.73 3.40 2.78 3.53 3.40 0.56 0 4.48E−05 −2.47 5.6 hsa-miR-369-hsa-miR-369- 4.90 6.08 6.78 5.92 0.95 100 3.56 2.71 4.47 4.36 4.36 2.532.92 2.62 3.53 3.45 0.80 0 1.21E−03 −2.47 5.5 5p 5p_st2 hsa-asg-13297hsa-asg-13297_st1 3.37 4.27 5.87 4.50 1.27 33 2.43 2.54 2.66 0.58 2.611.78 2.15 1.48 2.74 2.11 0.71 0 1.79E−03 −2.40 5.3 hsa-asg-9980hsa-asg-9980_st1 6.07 5.96 6.85 6.30 0.48 100 5.18 4.49 4.02 1.72 3.392.85 3.85 5.08 4.67 3.92 1.12 11 5.99E−03 −2.38 5.2 hsa-asg-2345hsa-asg-2345_st2 4.25 5.26 4.74 4.75 0.51 33 1.65 2.54 2.43 3.63 1.462.53 3.17 2.62 1.38 2.38 0.76 0 5.84E−04 −2.37 5.2 hsa-asg-8054hsa-asg-8054_st1 4.20 4.43 5.17 4.60 0.51 33 3.45 2.36 1.39 1.72 2.371.19 3.05 2.08 2.54 2.24 0.74 0 4.80E−04 −2.36 5.1 hsa-miR202hsa-miR202_st2 4.77 5.04 7.63 5.82 1.58 67 3.85 2.71 4.93 3.95 3.63 2.444.27 2.45 3.09 3.48 0.87 0 7.45E−03 −2.33 5.0 hsa-asg-11361hsa-asg-11361_st2 5.16 5.53 6.46 5.71 0.67 100 2.78 3.53 3.39 3.28 3.633.37 3.85 2.62 4.32 3.42 0.52 0 9.51E−05 −2.29 4.9 hsa-asg-8174hsa-asg-8174_st1 5.68 5.08 5.78 5.51 0.38 67 3.33 4.08 2.86 3.01 2.903.26 2.78 2.78 4.16 3.24 0.53 0 5.29E−05 −2.27 4.8 Has-cand206hsa-cand206_st1 4.55 5.01 5.27 4.95 0.37 67 2.25 1.96 3.79 3.63 2.692.17 3.59 1.29 2.74 2.68 0.86 0 1.46E−03 −2.27 4.8 hsa-miR-182hsa-miR-182_st1 11.4 11.4 11.7 11.5 0.21 100 10.2 10.0 8.85 9.82 9.379.55 8.99 8.57 8.13 9.28 0.70 100 3.32E−04 −2.25 4.8 hsa-asg-2027hsa-asg-2027_st1 5.21 6.95 6.41 6.19 0.89 100 3.56 3.53 1.66 5.23 4.474.24 4.27 4.21 4.54 3.97 1.01 0 6.94E−03 −2.22 4.7 hsa-asg-4914hsa-asg-4914_st1 4.59 5.14 4.64 4.79 0.31 33 2.78 3.29 0.13 3.87 3.102.07 2.48 3.44 2.33 2.61 1.09 0 7.73E−03 −2.18 4.5 hsa-miR-299-hsa-miR-299- 7.35 5.87 7.55 6.92 0.92 100 5.73 4.49 5.80 6.38 5.99 2.995.05 2.45 3.97 4.76 1.39 44 3.25E−02 −2.16 4.5 5p 5p_st2 hsa-miR-487ahsa-miR-487a_st2 4.04 4.90 5.05 4.67 0.54 33 2.25 2.87 2.66 3.40 2.193.26 0.91 2.27 2.92 2.53 0.75 0 1.11E−03 −2.14 4.4 hsa-miR-487ahsa-asg-6915_st1 5.13 5.67 6.01 5.60 0.45 100 4.09 3.64 3.91 3.40 3.893.78 3.68 3.74 3.65 3.75 0.20 0 1.09E−06 −1.85 3.6 hsa-asg-1826hsa-asg-1826_st2 4.59 4.83 5.24 4.89 0.33 100 3.33 3.02 2.18 3.57 2.532.17 2.92 3.13 1.86 2.75 0.59 0 1.57E−04 −2.14 4.4 Has-cand26hsa-cand26_st2 7.12 5.85 8.37 7.11 1.26 100 6.12 5.61 6.03 4.08 3.684.75 5.29 4.07 5.46 5.01 0.90 22 9.41E−03 −2.10 4.3 hsa-miR-433hsa-miR-433_st2 4.36 4.57 5.79 4.91 0.77 33 2.43 2.71 4.54 2.93 4.212.35 1.60 1.68 2.92 2.82 1.00 0 8.65E−03 −2.09 4.2 hsa-asg-10202hsa-asg-10202_st2 5.55 4.43 5.61 5.20 0.67 33 4.71 2.06 3.31 5.25 3.101.97 2.48 3.38 1.74 3.11 1.22 0 2.00E−02 −2.09 4.2 hsa-miR-134hsa-miR-134_st2 6.32 7.68 7.77 7.26 0.82 100 5.11 5.78 5.99 5.85 5.704.21 4.73 4.31 5.02 5.19 0.68 0 1.35E−03 −2.07 4.2 hsa-miR-1hsa-miR-1_st1 11.7 11.9 12.5 12.0 0.40 100 11.0 10.8 8.18 11.7 10.7 10.010.1 7.66 9.47 9.99 1.35 100 2.99E−02 −2.06 4.2 hsa-asg-10391hsa-asg-10391_st2 5.70 6.86 7.06 6.54 0.73 100 5.62 5.68 2.96 5.39 5.574.03 3.59 2.70 4.81 4.48 1.19 11 1.97E−02 −2.06 4.2 hsa-asg-7471hsa-asg-7471_st1 7.24 7.68 8.23 7.72 0.49 100 5.60 5.72 5.67 6.74 5.705.30 5.25 5.00 6.09 5.68 0.51 56 1.28E−04 −2.04 4.1 hsa-asg-1596hsa-asg-1596_st2 5.94 6.34 6.53 6.27 0.30 100 4.43 5.12 4.02 3.21 4.394.18 4.14 4.21 4.40 4.23 0.50 0 6.20E−05 −2.04 4.1 hsa-asg-10393Has-asg-10393_st1 3.87 5.01 5.24 4.71 0.73 67 2.78 1.54 2.43 3.91 2.832.85 1.79 3.54 2.54 2.69 0.75 0 2.31E−03 −2.02 4.0 hsa-asg-13135hsa-asg-13135_st1 7.12 5.96 8.36 7.15 1.20 100 5.62 5.67 5.24 4.91 4.794.75 5.16 4.47 5.60 5.13 0.43 22 1.04E−03 −2.02 4.0 hsa-asg-13284hsa-asg-13284_st1 5.67 5.57 6.39 5.88 0.45 100 4.90 2.06 3.60 5.83 4.852.85 4.73 3.78 2.44 3.89 1.28 0 2.82E−02 −1.98 4.0 hsa-miR-31hsa-miR-31_st2 9.86 11.4 12.2 11.1 1.20 100 10.4 9.79 8.95 10.1 8.718.60 7.73 8.68 10.1 9.23 0.91 100 1.35E−02 −1.95 3.9 hsa-miR-517ahsa-miR-517b_st1 6.82 5.35 6.39 6.19 0.76 100 4.99 5.61 6.51 3.87 3.223.82 4.07 3.91 2.54 4.28 1.22 22 3.16E−02 −1.90 3.7 hsa-miR-517ahsa-miR-517a_st1 6.81 5.08 5.97 5.95 0.87 100 4.85 5.37 6.26 3.57 3.103.82 4.20 3.74 1.86 4.09 1.29 0 4.43E−02 −1.87 3.6 hsa-miR-576hsa-miR-576_st2 2.69 4.62 5.37 4.23 1.38 33 2.25 2.87 3.06 1.72 2.011.97 1.97 2.08 3.09 2.34 0.53 0 4.47E−03 −1.89 3.7 hsa-miR-338hsa-miR-338_st1 5.37 6.28 6.88 6.18 0.76 100 5.11 4.08 5.57 4.86 4.042.17 4.78 3.44 4.89 4.33 1.03 11 1.81E−02 −1.85 3.6 hsa-asg-280hsa-asg-280_st1 4.10 4.43 5.99 4.84 1.01 33 3.21 2.71 4.47 2.13 2.532.17 1.79 3.44 4.54 3.00 1.00 0 2.01E−02 −1.84 3.6 hsa-asg-hsa-asg-14172_st1 5.78 5.57 6.68 6.01 0.59 100 5.07 2.71 4.47 5.45 4.812.77 3.59 3.74 5.18 4.20 1.03 0 1.79E−02 −1.81 3.5 14172 hsa-miR-485-hsa-miR23_st2 5.18 4.32 5.70 5.07 0.70 67 4.31 2.54 4.12 3.28 2.45 3.263.59 2.78 3.09 3.27 0.65 0 2.16E−03 −1.80 3.5 3p hsa-miR-379hsa-miR-379_st1 7.03 8.80 9.26 8.36 1.17 100 6.52 6.42 7.11 6.97 7.046.59 6.35 6.14 6.23 6.60 0.36 100 1.56E−03 −1.77 3.4 hsa-asg-6178hsa-asg-6178_st2 3.20 4.21 4.84 4.08 0.83 33 2.43 3.42 2.43 3.82 2.191.19 0.76 2.27 2.33 2.32 0.95 0 1.68E−02 −1.77 3.4 hsa-asg-1765hsa-asg-1765_st1 4.43 4.21 4.41 4.35 0.12 33 2.25 2.16 2.86 3.63 2.372.35 3.29 2.27 2.10 2.59 0.55 0 3.11E−04 −1.76 3.4 hsa-miR-382hsa-miR-382_st1 7.72 8.68 9.06 8.49 0.69 100 7.17 6.85 7.35 7.13 7.436.56 6.29 5.93 5.81 6.72 0.61 78 1.76E−03 −1.76 3.4 hsa-asg-3711hsa-asg-3711_st2 6.96 6.77 7.14 6.96 0.19 100 5.70 6.17 5.12 5.53 5.145.25 4.54 4.44 4.87 5.19 0.55 0 3.61E−04 −1.76 3.4 hsa-asg-hsa-asg-12690_st1 5.05 3.81 5.39 4.75 0.83 67 3.76 2.87 2.66 3.40 3.492.85 2.92 2.93 2.10 3.00 0.49 0 1.07E−03 −1.75 3.4 12690 hsa-miR-376bhsa-miR-376b_st1 3.37 5.08 3.63 4.03 0.92 33 1.85 2.16 4.12 3.34 2.691.01 2.78 2.53 0.21 2.30 1.18 0 4.49E−02 −1.73 3.3 hsa-asg-378hsa-asg-378_st1 4.55 4.97 3.82 4.45 0.59 33 2.25 2.71 2.18 4.71 2.832.99 1.08 2.78 3.09 2.74 0.96 0 1.72E−02 −1.71 3.3 hsa-asg-2399hsa-asg-2399_st2 3.20 5.04 4.53 4.26 0.95 33 3.07 3.42 2.86 3.01 2.192.53 1.08 2.27 2.54 2.55 0.68 0 6.26E−03 −1.71 3.3 hsa-miR-101hsa-miR-101_st1 6.18 7.95 7.41 7.18 0.91 100 7.18 6.53 4.21 5.98 6.524.70 4.86 4.35 5.09 5.49 1.08 44 3.61E−02 −1.69 3.2 hsa-miR-218hsa-miR-218_st2 9.61 10.7 11.1 10.4 0.79 100 9.62 9.27 8.82 9.68 9.168.64 7.76 7.45 8.75 8.80 0.77 100 8.28E−03 −1.69 3.2 hsa-miR-363hsa-miR-363_st2 11.0 11.4 11.9 11.4 0.47 100 10.6 10.0 9.90 10.2 9.049.40 10.0 9.11 9.94 9.81 0.52 100 5.61E−04 −1.69 3.2 hsa-asg-1701hsa-asg-1701_st2 5.24 5.75 4.41 5.14 0.68 33 4.90 3.16 3.91 4.63 3.682.99 3.29 2.93 1.62 3.46 0.98 0 2.19E−02 −1.68 3.2 hsa-miR-154*hsa-miR-154-_st2 5.68 6.08 6.26 6.01 0.30 100 5.83 3.53 4.21 4.99 4.633.95 4.27 3.20 4.40 4.33 0.78 11 5.35E−03 −1.67 3.2 hsa-asg-8411hsa-asg-8411_st2 6.06 6.13 7.03 6.41 0.54 100 5.92 4.49 5.04 6.08 5.094.87 4.14 2.93 4.07 4.73 0.97 22 1.94E−02 −1.67 3.2 hsa-asg-hsa-asg-10881_st1 6.74 6.02 7.90 6.89 0.95 100 5.73 5.04 6.06 5.43 4.795.46 4.20 5.04 5.23 5.22 0.54 22 3.07E−03 −1.67 3.2 10881 hsa-asg-548hsa-asg-548_st1 4.55 4.43 5.20 4.73 0.42 33 3.07 1.96 3.54 3.52 2.831.97 3.40 2.78 4.60 3.07 0.83 0 8.62E−03 −1.65 3.1 hsa-miR-494hsa-miR-494_st1 6.95 9.16 8.93 8.35 1.21 100 6.40 7.30 7.22 6.96 6.637.66 5.73 5.86 6.60 6.71 0.65 100 1.12E−02 −1.64 3.1 hsa-miR-494hsa-miR61_st2 6.79 8.48 8.35 7.88 0.94 100 6.83 6.55 6.85 6.43 6.38 7.105.40 5.79 6.89 6.47 0.55 89 8.75E−03 −1.41 2.7 hsa-asg-hsa-asg-13205_st1 7.15 8.56 8.28 8.00 0.75 100 7.02 6.39 7.09 7.51 6.906.04 6.76 5.59 4.40 6.41 0.95 67 2.62E−02 −1.59 3.0 13205 hsa-miR-9*hsa-miR-9-_st2 5.75 7.41 8.33 7.16 1.31 100 6.20 6.10 5.57 6.11 5.415.87 4.94 4.73 5.23 5.57 0.54 44 1.03E−02 −1.59 3.0 hsa-miR-497hsa-miR-497_st1 7.86 8.79 9.18 8.61 0.68 100 7.43 7.60 6.23 8.75 7.866.49 6.51 5.62 6.69 7.02 0.96 33 2.64E−02 −1.59 3.0 hsa-miR-497hsa-miR43_st1 8.44 9.72 10.0 9.39 0.83 100 8.20 8.56 7.51 9.37 8.88 7.787.45 7.13 7.83 8.08 0.74 89 2.63E−02 −1.31 2.5 hsa-miR-212hsa-miR-212_st2 6.34 6.73 7.74 6.93 0.72 100 6.27 5.73 6.17 5.72 5.175.11 4.59 4.98 4.76 5.39 0.61 11 4.31E−03 −1.55 2.9 hsa-miR-132hsa-miR-132_st1 9.30 9.22 11.0 9.87 1.05 100 9.72 8.56 8.33 8.35 8.598.08 8.02 8.44 6.88 8.33 0.74 89 1.70E−02 −1.54 2.9 hsa-miR-222hsa-miR-222_st2 10.7 11.1 11.9 11.2 0.61 100 10.4 9.56 9.54 10.2 9.759.33 9.52 9.38 9.86 9.73 0.37 100 3.50E−04 −1.52 2.9 hsa-asg-hsa-asg-12240_st1 5.21 5.17 5.69 5.36 0.29 100 4.17 3.16 3.91 4.08 4.892.69 3.05 4.41 4.40 3.86 0.73 0 7.31E−03 −1.49 2.8 12240 hsa-miR-194hsa-miR-194_st2 11.1 9.33 10.7 10.4 0.96 100 9.83 9.37 8.91 9.35 8.958.34 8.55 8.21 9.05 8.95 0.52 100 6.36E−03 −1.46 2.7 hsa-cand330hsa-cand330_st2 5.08 5.79 6.13 5.67 0.54 100 4.76 5.04 3.54 4.71 5.154.18 2.64 3.99 3.97 4.22 0.80 11 1.68E−02 −1.45 2.7 hsa-asg-191hsa-asg-191_st1 4.75 5.55 5.05 5.12 0.40 33 3.33 3.92 2.86 3.57 3.854.10 4.00 3.20 4.84 3.74 0.58 0 3.80E−03 −1.38 2.6 hsa-miR-574hsa-miR-574_st1 10.1 10.2 10.8 10.4 0.38 100 9.35 8.42 9.39 10.2 9.968.12 9.20 8.06 8.47 9.02 0.80 100 1.83E−02 −1.37 2.6 hsa-asg-hsa-asg-14150_st1 6.92 8.37 8.14 7.81 0.78 67 7.05 6.35 7.01 7.73 6.675.80 5.79 5.15 6.39 6.44 0.78 11 2.52E−02 −1.37 2.6 14150 hsa-miR-215hsa-miR-215_st1 8.08 6.02 7.15 7.08 1.03 100 6.96 5.82 5.71 6.66 5.544.84 5.51 5.11 5.23 5.71 0.70 33 2.43E−02 −1.37 2.6 hsa-asg-7417hsa-asg-7417_st1 5.41 4.21 6.26 5.29 1.03 67 4.55 3.36 4.50 5.28 4.043.26 3.59 3.59 3.17 3.93 0.72 0 2.72E−02 −1.36 2.6 hsa-asg-hsa-asg-11443_st1 3.74 5.55 4.14 4.48 0.95 33 4.17 3.64 2.86 2.69 3.542.69 3.40 2.78 2.33 3.12 0.59 0 1.33E−02 −1.36 2.6 11443 hsa-asg-7540hsa-asg-7540_st2 5.10 5.32 5.57 5.33 0.23 100 3.76 4.43 3.06 3.52 4.144.97 3.93 4.47 3.97 4.03 0.56 0 3.54E−03 −1.30 2.5 hsa-miR-9hsa-miR-9_st2 4.25 3.56 6.06 4.62 1.29 33 3.45 3.53 2.86 3.28 3.63 2.173.50 3.32 4.16 3.32 0.55 0 2.78E−02 −1.30 2.5 hsa-asg-8511hsa-asg-8511_st2 3.84 5.79 4.35 4.66 1.01 33 3.66 3.92 2.86 3.57 1.923.78 2.92 4.07 3.53 3.36 0.68 0 2.74E−02 −1.30 2.5 hsa-asg-hsa-asg-10360_st1 3.99 4.94 4.93 4.62 0.55 33 3.45 3.42 3.06 4.66 3.723.26 3.17 2.45 2.74 3.32 0.63 0 1.02E−02 −1.29 2.5 10360 hsa-asg-8380hsa-asg-8380_st2 4.70 5.08 6.11 5.30 0.73 100 4.17 3.42 3.91 5.56 4.584.18 2.78 2.93 4.54 4.01 0.87 0 4.49E−02 −1.29 2.4 hsa-miR-214hsa-miR-214_st1 10.4 10.9 11.3 10.9 0.49 100 9.76 9.55 9.76 10.9 10.39.93 9.27 8.44 8.52 9.62 0.81 100 2.97E−02 −1.28 2.4 hsa-asg-hsa-asg-10291_st2 4.49 6.07 4.97 5.18 0.81 33 4.17 4.58 4.07 4.04 3.632.99 3.85 3.49 4.28 3.90 0.47 0 6.30E−03 −1.28 2.4 10291 hsa-asg-hsa-asg-10883_st1 8.02 9.06 8.16 8.41 0.56 100 7.64 7.18 7.34 8.22 8.036.58 6.97 5.74 6.63 7.15 0.78 89 2.79E−02 −1.26 2.4 10883 hsa-miR-99bhsa-miR-99b_st1 11.4 11.3 12.4 11.7 0.59 100 11.0 10.5 10.5 11.4 11.110.1 10.1 9.52 10.3 10.5 0.59 100 1.12E−02 −1.22 2.3 hsa-miR-99bhsa-asg-14196_st2 11.5 11.3 12.5 11.8 0.64 100 11.2 10.6 10.2 11.6 11.310.3 10.3 9.55 10.5 10.7 0.65 100 2.85E−02 −1.10 2.1 hsa-asg-hsa-asg-14048_st1 4.87 5.57 6.07 5.50 0.60 67 4.43 4.95 3.54 3.63 5.033.26 4.59 5.15 4.07 4.29 0.70 0 2.44E−02 −1.21 2.3 14048 hsa-asg-360hsa-asg-360_st2 4.99 5.62 5.84 5.48 0.44 67 4.49 3.53 4.02 5.45 4.773.64 4.00 4.35 4.32 4.28 0.59 0 9.57E−03 −1.20 2.3 hsa-asg-3821hsa-asg-3821_st2 4.77 5.89 5.58 5.41 0.58 33 3.45 5.19 3.39 4.63 3.105.11 4.20 4.14 4.78 4.22 0.77 0 3.59E−02 −1.19 2.3 hsa-asg-hsa-asg-13308_st2 10.3 10.0 11.4 10.6 0.72 100 10.5 9.46 9.58 9.81 9.178.63 9.51 8.96 9.11 9.43 0.56 100 1.33E−02 −1.19 2.3 13308 hsa-asg-7465hsa-asg-7465_st2 6.09 5.75 6.38 6.07 0.31 100 4.66 4.37 4.02 6.18 5.094.21 5.16 5.22 5.05 4.88 0.66 11 1.43E−02 −1.19 2.3 hsa-asg-hsa-asg-13279_st1 7.84 8.38 9.03 8.42 0.60 100 7.58 7.47 7.55 8.17 7.246.59 6.87 7.00 6.68 7.24 0.51 100 7.21E−03 −1.18 2.3 13279 hsa-asg-hsa-asg-13270_st2 4.41 6.39 4.64 5.15 1.09 33 3.93 3.83 3.79 4.97 4.604.31 3.50 3.83 3.09 3.98 0.57 0 3.26E−02 −1.16 2.2 13270 hsa-asg-hsa-asg-11883_st1 6.51 7.56 7.65 7.24 0.63 100 5.92 6.06 6.67 6.74 6.186.02 6.12 4.58 6.44 6.08 0.63 78 2.08E−02 −1.16 2.2 11883 hsa-asg-4366hsa-asg-4366_st1 3.81 4.21 4.74 4.25 0.47 33 3.45 3.16 3.23 3.82 3.102.85 2.15 2.62 3.53 3.10 0.51 0 6.09E−03 −1.15 2.2 hsa-asg-3251hsa-asg-3251_st1 4.99 3.73 5.37 4.70 0.86 67 4.24 4.16 4.02 2.85 3.543.95 2.92 3.07 3.25 3.56 0.55 0 2.07E−02 −1.14 2.2 hsa-miR-192hsa-miR-192_st2 11.4 9.87 11.0 10.8 0.84 100 10.8 9.87 10.1 10.0 9.588.72 9.33 8.80 9.65 9.66 0.66 100 3.45E−02 −1.14 2.2 hsa-miR-331hsa-miR-331_st2 8.22 8.80 9.96 8.99 0.88 100 9.04 7.57 8.35 8.79 7.697.09 7.70 7.28 7.20 7.86 0.71 100 4.56E−02 −1.14 2.2 hsa-miR-27bhsa-miR-27b_st1 13.0 13.4 13.7 13.4 0.31 100 12.57 12.35 12.07 13.2912.86 11.94 11.94 11.06 12.51 12.29 0.64 100 1.65E−02 −1.13 2.2 hsa-asg-hsa-asg-13797_st2 4.04 5.53 3.99 4.52 0.87 33 2.43 3.53 2.86 3.01 3.443.69 2.92 4.58 4.16 3.40 0.68 0 4.22E−02 −1.12 2.2 13797 hsa-miR-324-hsa-miR-324- 8.04 9.68 9.45 9.06 0.89 100 7.92 8.13 8.33 8.27 7.80 7.427.82 7.87 7.98 7.95 0.27 100 5.23E−03 −1.11 2.2 5p 5p_st1 hsa-asg-hsa-asg-10674_st1 5.12 6.16 6.57 5.95 0.75 67 4.31 4.49 5.24 5.42 5.215.30 4.73 4.35 4.67 4.86 0.44 0 9.72E−03 −1.10 2.1 10674 hsa-asg-hsa-asg-14233_st1 9.72 10.5 10.7 10.3 0.52 100 9.45 9.26 9.23 10.1 9.448.89 9.05 8.87 8.89 9.25 0.41 100 4.07E−03 −1.07 2.1 14233 hsa-asg-5304hsa-asg-5304_st1 10.2 10.1 11.4 10.6 0.68 100 10.6 9.48 9.75 10.1 9.408.81 9.58 9.07 9.15 9.56 0.56 100 2.09E−02 −1.07 2.1 hsa-miR-409-hsa-miR-409- 6.25 6.89 6.95 6.69 0.39 100 5.14 5.04 6.67 6.09 6.11 5.175.92 5.45 5.27 5.65 0.57 44 1.53E−02 −1.04 2.1 5p 5p_st1 hsa-asg-3998hsa-asg-3998_st1 9.19 9.86 10.3 9.81 0.60 100 8.63 8.58 8.70 9.41 8.778.37 9.21 8.76 8.49 8.77 0.34 100 3.11E−03 −1.04 2.1 hsa-asg-Has-asg-12515_st2 3.78 5.53 5.79 5.03 1.10 67 4.02 4.30 3.06 4.12 4.604.37 3.68 4.14 3.77 4.01 0.46 0 3.68E−02 −1.02 2.0 12515 hsa-miR-30e-hsa-miR-30e- 10.0 10.6 11.0 10.5 0.54 100 10.4 9.64 9.71 10.5 9.81 9.239.23 8.15 9.50 9.58 0.70 100 4.93E−02 −1.00 2.0 5p 5p_st2 SD, standarddeviation; %, percentage of samples expressing miRNA above backgroundlevels; Log2Diff, difference in VSN-transformed expression bewteen PROand LNneg samples.

Example 25 miRNAs Differentally Expressed Between Normal Lung Tissue andNormal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous lung cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal lung tissuethan in cancer-negative lymph nodes.

Total RNA, purified from three separate normal human lung tissue samples(FirstChoice® Human Lung Total RNA, cat. no. AM7968; Ambion, Austin,Tex., USA) and from nine normal human lymph node samples (FirstChoice®Human Lymph Node Total RNA, cat. no. AM7894; Ambion, Austin, Tex., USA)was purchased from Ambion. miRNA purification and labeling andmicroarray expression analyses were performed as described above inExample 2.

The inventors identified 182 miRNAs (72 verified, 110 predicted) withsignificant differential expression between normal lung and normal lymphnodes (log 2Diff lung vs LN neg≧1 and p-value<0.05) (Table 24). Amongthe verified miRNAs, hsa-miR-375 had an average expression level over100-fold higher in lung tissue samples; hsa-miR-429, -205 and -203) hadaverage expression levels that were over 50-fold higher in lung samples;6 miRNAs (hsa-miR-34c, -200a, -200b, -200c, -184 and -141) had averageexpression levels that were between 20- and 50-fold higher in lungsamples; 4 miRNAs (hsa-miR-449, -605, -338 and -187) had averageexpression levels that were between 10- and 20-fold higher in lungsamples; and 11 miRNAs had average expression levels that were between5- and 10-fold higher in lung samples compared to normal lymph nodes.Among the predicted miRNAs, hsa-asg-13231_st1 had an average expressionlevel that was over 50-fold higher in lung samples; hsa-asg-4439-st2 andhsa-cand150_st2 had average expression levels that were between 25- and45-fold higher in lung samples; 6 miRNAs had average expression levelsthat were between 10- and 20-fold higher in lung samples; and 18 miRNAshad average expression levels that were between 5- and 10-fold higher inlung tissue samples compared to normal lymph nodes.

TABLE 24 MicroRNAs Differentially Expressed Between Normal Lung Samples(LUN) and Cancer-Negative Lymph Node Samples (LNneg) from Patients. LUNLNneg Micro RNA Probe ID 1 2 3 M SD % 1 2 3 4 5 6 7 8 9 M SD % ttestlog2Diff Fold hsa-miR-375 hsa-miR-375_st1 12.1 10.6 10.2 11.0 0.99 1005.96 4.93 6.23 2.85 2.97 3.69 0.99 4.63 6.39 4.29 1.82 0 1.32E−04 6.75107 hsa-miR-429 hsa-miR-429_st1 8.79 9.71 8.77 9.09 0.54 100 3.45 1.543.67 2.13 2.37 2.69 2.32 2.45 5.39 2.89 1.14 0 4.61E−06 6.20 73.7hsa-miR-205 hsa-miR-205_st1 9.96 11.9 10.5 10.8 1.01 100 2.93 5.91 5.642.03 3.59 6.11 3.40 5.32 7.76 4.74 1.85 33 3.36E−04 6.08 67.8hsa-miR-203 hsa-miR-203_st2 12.1 12.7 12.8 12.5 0.37 100 7.33 5.89 7.218.24 6.45 5.41 5.19 6.07 9.36 6.79 1.37 78 3.82E−05 5.76 54.3hsa-asg-13231 hsa-asg-13231_st1 9.35 9.39 9.03 9.26 0.20 100 3.93 4.164.21 4.46 2.97 3.43 1.79 2.08 4.47 3.50 1.01 0 2.59E−06 5.76 54.2hsa-asg-4439 hsa-asg-4439_st2 8.99 8.74 8.50 8.74 0.25 100 3.85 4.373.23 2.69 1.64 3.69 2.78 3.74 4.07 3.34 0.85 0 9.38E−07 5.40 42.3hsa-miR-34c hsa-miR-34c_st2 7.45 10.3 9.17 8.99 1.46 100 1.85 2.54 3.543.68 3.63 3.06 3.50 3.74 6.85 3.60 1.37 11 1.70E−04 5.39 42.0hsa-miR-200a hsa-miR-200a_st2 10.3 10.7 10.4 10.5 0.20 100 6.27 5.086.49 5.72 5.29 4.60 4.07 4.78 7.78 5.56 1.14 67 2.60E−05 4.97 31.4hsa-miR-200b hsa-miR-200b_st1 12.0 12.9 12.6 12.5 0.45 100 7.62 7.808.90 6.69 6.82 6.87 6.51 7.65 10.4 7.70 1.26 100 8.48E−05 4.85 28.9hsa-miR-200c hsa-miR-200c_st1 13.6 13.9 13.5 13.7 0.22 100 9.60 8.8410.3 8.18 8.15 8.14 7.98 8.89 11.5 9.07 1.21 100 7.06E−05 4.68 25.7hsa-cand150 hsa-cand150_st2 8.86 8.25 7.79 8.30 0.53 100 6.27 3.74 2.432.51 3.16 3.48 2.64 3.99 4.47 3.63 1.21 11 8.71E−05 4.67 25.4hsa-miR-184 hsa-miR-184_st1 8.09 9.87 7.69 8.55 1.16 100 5.51 4.55 4.124.30 3.93 5.17 1.60 3.83 4.16 4.13 1.10 11 1.39E−04 4.42 21.5hsa-miR-141 hsa-miR-141_st1 8.32 9.47 7.96 8.58 0.79 100 5.07 3.83 4.934.43 4.39 2.85 3.85 3.83 4.54 4.19 0.68 0 2.90E−06 4.39 21.0 hsa-miR-449hsa-miR-449_st2 5.59 8.67 6.12 6.79 1.64 67 2.61 1.54 4.54 3.08 3.631.68 0.91 1.68 4.73 2.71 1.37 0 1.63E−03 4.08 16.9 hsa-asg-9269hsa-asg-9269_st2 6.08 8.53 6.25 6.95 1.37 100 2.61 2.54 4.68 2.93 2.612.69 2.64 2.27 3.97 2.99 0.80 0 8.55E−05 3.96 15.5 hsa-miR-605hsa-miR-605_st1 6.58 6.82 6.09 6.50 0.37 100 2.61 2.16 3.06 0.75 1.742.17 2.92 3.07 4.47 2.55 1.03 0 8.83E−05 3.95 15.4 hsa-miR-338hsa-miR-338_st1 7.63 8.50 8.18 8.10 0.44 100 5.11 4.08 5.57 4.86 4.042.17 4.78 3.44 4.89 4.33 1.03 11 1.34E−04 3.78 13.7 hsa-asg-4438hsa-asg-4438_st2 8.71 8.45 7.88 8.35 0.42 100 4.55 4.55 5.04 4.55 4.144.55 4.20 4.83 5.35 4.64 0.38 0 5.62E−08 3.71 13.1 hsa-miR-187hsa-miR-187_st1 7.12 7.40 6.69 7.07 0.36 100 4.76 2.79 2.76 3.77 3.223.37 3.05 3.38 3.17 3.37 0.61 0 1.90E−06 3.70 13.0 hsa-asg-8428hsa-asg-8428_st2 4.99 6.02 5.36 5.46 0.52 100 2.25 1.96 2.66 2.03 2.531.01 0.02 2.17 1.62 1.80 0.83 0 3.58E−05 3.65 12.6 hsa-asg-10131hsa-asg-10131_st2 6.99 7.32 6.35 6.89 0.49 100 3.33 3.16 3.54 2.51 2.533.26 2.64 3.20 5.09 3.25 0.78 0 2.27E−05 3.64 12.4 hsa-asg-1794hsa-asg-1794_st1 4.95 5.74 5.43 5.37 0.40 67 1.85 1.96 2.66 1.72 2.191.01 2.15 1.48 2.92 1.99 0.58 0 3.21E−06 3.38 10.4 hsa-asg-7637hsa-asg-7637_st2 7.58 6.98 6.85 7.14 0.39 100 3.66 2.54 4.47 3.46 3.934.03 3.29 3.64 5.27 3.81 0.76 0 3.36E−05 3.33 10.1 hsa-miR-449bhsa-miR-449b_st1 5.32 8.42 5.83 6.52 1.66 100 1.85 3.29 4.47 2.51 3.592.85 2.78 3.20 4.32 3.21 0.84 0 8.14E−04 3.32 10.0 hsa-asg-11715hsa-asg-11715_st2 4.77 5.25 5.56 5.20 0.40 67 1.85 2.36 2.18 1.51 2.111.78 0.62 1.48 3.39 1.92 0.75 0 3.52E−05 3.28 9.7 hsa-asg-1296hsa-asg-1296_st1 6.07 7.42 6.09 6.53 0.77 100 3.07 2.54 4.81 2.42 2.532.77 3.17 3.91 4.16 3.27 0.84 0 1.50E−04 3.26 9.6 hsa-asg-3822hsa-asg-3822_st2 5.83 6.16 5.90 5.96 0.18 100 2.05 3.02 3.67 2.03 2.452.17 1.60 2.93 4.78 2.75 0.99 0 2.89E−04 3.22 9.3 hsa-asg-13135hsa-asg-13135_st1 8.53 7.74 8.74 8.34 0.53 100 5.62 5.67 5.24 4.91 4.794.75 5.16 4.47 5.60 5.13 0.43 22 9.42E−07 3.21 9.2 hsa-asg-13705hsa-asg-13705_st1 5.50 5.74 5.17 5.47 0.28 100 1.65 0.95 3.79 2.33 2.692.35 0.35 3.20 3.25 2.29 1.12 0 8.23E−04 3.19 9.1 Has-cand26hsa-cand26_st2 8.22 7.55 8.69 8.15 0.57 100 6.12 5.61 6.03 4.08 3.684.75 5.29 4.07 5.46 5.01 0.90 22 2.39E−04 3.14 8.8 hsa-asg-13997hsa-asg-13997_st2 5.70 4.89 6.42 5.67 0.77 100 3.07 3.29 2.18 3.01 2.192.35 1.25 3.20 2.33 2.54 0.66 0 4.43E−05 3.13 8.7 hsa-asg-10247hsa-asg-10247_st1 5.45 5.93 5.36 5.58 0.31 100 2.78 2.16 4.02 1.72 2.192.35 0.35 3.20 3.97 2.53 1.14 0 1.26E−03 3.05 8.3 hsa-miR-96hsa-miR-96_st1 5.52 7.05 5.89 6.15 0.80 100 4.99 3.42 3.06 3.01 4.112.85 3.05 1.88 3.09 3.27 0.86 11 4.79E−04 2.88 7.4 hsa-miR-183hsa-miR-183_st1 9.72 10.4 9.06 9.76 0.71 100 7.66 6.76 6.71 7.93 7.565.83 7.52 6.32 6.65 6.99 0.70 89 1.58E−04 2.76 6.8 hsa-miR-34bhsa-miR-34b_st2 6.69 9.66 8.25 8.20 1.48 100 4.76 5.12 6.41 6.24 5.055.24 4.73 5.11 6.41 5.45 0.70 56 1.10E−03 2.75 6.7 hsa-asg-10881hsa-asg-10881_st1 7.85 7.70 8.28 7.94 0.30 100 5.73 5.04 6.06 5.43 4.795.46 4.20 5.04 5.23 5.22 0.54 22 1.04E−05 2.72 6.6 hsa-miR-551bhsa-miR167_st2 6.03 7.60 7.65 7.09 0.92 100 5.25 4.43 3.79 5.85 3.394.03 4.82 3.54 4.32 4.38 0.81 22 6.40E−04 2.71 6.6 hsa-miR-551bhsa-miR-551b_st1 5.40 7.26 7.37 6.68 1.11 100 4.81 4.86 4.87 4.76 4.002.69 4.14 2.93 4.40 4.16 0.83 0 1.76E−03 2.52 5.7 hsa-asg-14238hsa-asg-14238_st1 5.32 7.67 5.36 6.12 1.35 100 3.76 4.61 3.79 3.28 4.242.99 3.85 1.48 2.74 3.42 0.93 0 2.76E−03 2.70 6.5 hsa-miR-576hsa-miR-576_st2 4.02 5.96 5.09 5.02 0.97 67 2.25 2.87 3.06 1.72 2.011.97 1.97 2.08 3.09 2.34 0.53 0 8.93E−05 2.69 6.4 hsa-miR-200a*hsa-miR-200a-_st2 5.09 5.49 5.58 5.39 0.26 100 3.07 2.87 2.18 3.91 2.611.78 2.15 3.20 2.92 2.74 0.65 0 5.32E−05 2.64 6.2 hsa-asg-5119hsa-asg-5119_st1 4.99 5.42 5.09 5.17 0.23 67 2.43 2.71 1.92 1.93 2.012.53 2.92 2.62 3.65 2.53 0.55 0 1.42E−05 2.64 6.2 hsa-miR-582hsa-miR-582_st1 6.79 7.85 6.79 7.14 0.61 100 5.85 2.16 4.54 5.96 4.894.10 4.07 4.35 4.67 4.51 1.12 22 3.41E−03 2.63 6.2 hsa-miR-493-5phsa-miR-493-5p_st1 6.59 7.74 7.44 7.26 0.59 100 4.37 3.83 6.88 6.18 6.333.69 4.38 1.88 4.60 4.68 1.56 33 2.19E−02 2.57 5.9 hsa-asg-4219hsa-asg-4219_st1 4.99 5.82 4.23 5.01 0.79 33 3.45 1.75 1.66 1.51 2.281.78 2.92 2.78 3.97 2.46 0.87 0 1.21E−03 2.56 5.9 hsa-asg-11380hsa-asg-11380_st2 4.85 5.22 5.03 5.03 0.19 33 2.93 1.75 3.06 2.51 2.191.97 2.48 2.27 3.09 2.47 0.48 0 5.01E−06 2.56 5.9 hsa-miR-218hsa-miR-218_st2 10.7 11.5 11.7 11.3 0.51 100 9.62 9.27 8.82 9.68 9.168.64 7.76 7.45 8.75 8.80 0.77 100 3.49E−04 2.55 5.9 hsa-miR-189hsa-miR-189_st1 7.57 8.21 7.94 7.91 0.32 100 6.08 5.79 4.93 7.23 6.184.55 4.94 3.54 4.95 5.36 1.08 44 2.92E−03 2.55 5.9 Has-cand852hsa-cand852_st2 5.02 5.25 5.45 5.24 0.22 67 3.07 1.96 2.86 3.01 2.282.69 2.92 2.08 3.53 2.71 0.51 0 1.06E−05 2.53 5.8 hsa-asg-4978hsa-asg-4978_st2 4.73 5.10 5.20 5.01 0.25 33 1.65 2.87 2.86 1.72 2.011.97 2.78 2.62 4.16 2.52 0.79 0 3.74E−04 2.49 5.6 hsa-asg-14172hsa-asg-14172_st1 5.55 6.90 7.47 6.64 0.99 67 5.07 2.71 4.47 5.45 4.812.77 3.59 3.74 5.18 4.20 1.03 0 5.08E−03 2.44 5.4 hsa-asg-9980hsa-asg-9980_st1 6.73 5.69 6.64 6.35 0.58 100 5.18 4.49 4.02 1.72 3.392.85 3.85 5.08 4.67 3.92 1.12 11 5.50E−03 2.44 5.4 hsa-asg-1596hsa-asg-1596_st2 6.68 6.62 6.66 6.66 0.03 100 4.43 5.12 4.02 3.21 4.394.18 4.14 4.21 4.40 4.23 0.50 0 9.61E−06 2.42 5.4 hsa-miR-101hsa-miR-101_st1 6.93 8.55 7.91 7.80 0.81 100 7.18 6.53 4.21 5.98 6.524.70 4.86 4.35 5.09 5.49 1.08 44 7.42E−03 2.30 4.9 hsa-asg-5205hsa-asg-5205_st1 10.0 8.36 9.60 9.32 0.86 100 8.03 7.79 5.04 7.50 8.306.93 6.69 7.60 5.39 7.03 1.15 44 1.06E−02 2.29 4.9 hsa-asg-4915hsa-asg-4915_st1 4.02 5.42 6.14 5.19 1.08 33 2.43 3.83 2.18 2.60 3.632.92 2.78 2.08 4.47 2.99 0.81 0 3.59E−03 2.20 4.6 hsa-asg-5996hsa-asg-5996_st2 10.0 7.31 9.24 8.88 1.42 33 8.10 7.67 5.28 7.68 8.037.00 6.23 6.39 3.77 6.68 1.44 0 4.50E−02 2.20 4.6 hsa-asg-3912hsa-asg-3912_st1 3.66 5.42 4.23 4.44 0.90 33 1.65 2.54 2.86 1.61 2.191.19 2.92 2.17 3.53 2.30 0.74 0 2.03E−03 2.14 4.4 hsa-cand710hsa-cand710_st2 10.0 8.29 9.23 9.18 0.87 100 8.30 7.44 5.89 7.66 8.257.44 6.15 7.29 5.09 7.06 1.10 44 1.31E−02 2.13 4.4 hsa-asg-11361hsa-asg-11361_st2 5.50 6.02 5.09 5.54 0.47 100 2.78 3.53 3.39 3.28 3.633.37 3.85 2.62 4.32 3.42 0.52 0 9.36E−05 2.12 4.3 hsa-asg-11160hsa-asg-11160_st2 4.94 5.86 5.27 5.36 0.47 67 2.78 3.16 3.39 3.08 2.902.69 2.48 3.54 5.14 3.24 0.79 0 1.51E−03 2.12 4.3 hsa-miR-592hsa-miR-592_st1 3.95 3.86 7.39 5.07 2.01 33 4.31 2.54 2.86 3.15 1.831.78 3.05 2.62 4.54 2.96 0.96 0 2.91E−02 2.10 4.3 hsa-asg-13908hsa-asg-13908_st2 3.95 5.39 4.76 4.70 0.72 33 2.05 2.54 3.67 2.51 1.462.85 2.64 2.45 3.77 2.66 0.72 0 1.70E−03 2.04 4.1 hsa-asg-280hsa-asg-280_st1 4.51 5.39 5.20 5.04 0.46 67 3.21 2.71 4.47 2.13 2.532.17 1.79 3.44 4.54 3.00 1.00 0 7.52E−03 2.04 4.1 hsa-miR-495hsa-miR-495_st1 7.02 7.60 7.26 7.29 0.29 100 6.02 4.81 6.97 6.26 6.324.43 5.16 2.45 4.89 5.26 1.35 56 3.05E−02 2.04 4.1 hsa-asg-13205hsa-asg-13205_st1 7.86 9.04 8.39 8.43 0.59 100 7.02 6.39 7.09 7.51 6.906.04 6.76 5.59 4.40 6.41 0.95 67 6.78E−03 2.02 4.1 hsa-asg-8174hsa-asg-8174_st1 5.18 5.84 4.76 5.26 0.55 67 3.33 4.08 2.86 3.01 2.903.26 2.78 2.78 4.16 3.24 0.53 0 2.15E−04 2.02 4.1 hsa-asg-14015hsa-asg-14015_st1 7.26 5.06 6.93 6.42 1.19 67 5.11 5.32 5.30 5.02 5.434.55 3.59 2.70 2.83 4.43 1.10 0 2.31E−02 1.99 4.0 hsa-miR-598hsa-miR-598_st2 7.22 7.80 7.67 7.56 0.30 100 6.71 5.98 5.89 6.87 5.975.59 5.59 3.07 5.05 5.64 1.11 56 1.65E−02 1.93 3.8 hsa-asg-13077hsa-asg-13077_st1 7.94 5.22 6.91 6.69 1.37 100 5.98 5.12 3.23 5.27 6.035.05 4.54 3.91 3.97 4.79 0.95 44 2.20E−02 1.90 3.7 hsa-miR-19ahsa-miR-19a_st1 4.73 5.69 3.82 4.74 0.94 33 4.61 3.42 2.86 3.15 3.632.53 1.60 1.29 2.54 2.85 1.02 0 1.76E−02 1.90 3.7 hsa-miR-182hsa-miR-182_st1 11.0 11.8 10.5 11.1 0.65 100 10.2 10.0 8.85 9.82 9.379.55 8.99 8.57 8.13 9.28 0.70 100 2.09E−03 1.89 3.7 hsa-miR-18ahsa-miR-18a_st1 6.70 8.28 7.25 7.41 0.80 100 5.78 6.02 7.10 5.97 4.534.14 5.69 5.67 4.89 5.53 0.89 44 9.06E−03 1.88 3.7 hsa-miR-181a*hsa-miR-213_st1 6.78 8.30 8.03 7.70 0.81 100 6.30 6.22 5.67 5.72 5.455.43 5.32 5.79 6.55 5.83 0.43 56 3.43E−04 1.88 3.7 hsa-asg-14150hsa-asg-14150_st1 7.74 8.88 8.25 8.29 0.57 33 7.05 6.35 7.01 7.73 6.675.80 5.79 5.15 6.39 6.44 0.78 11 3.97E−03 1.85 3.6 hsa-miR-487bhsa-miR-487b_st2 6.70 7.79 7.84 7.44 0.64 100 6.06 5.84 6.97 6.90 6.255.40 4.90 3.74 4.40 5.61 1.10 67 2.30E−02 1.84 3.6 hsa-miR-199bhsa-miR-199b_st2 9.96 11.82 10.7 10.8 0.93 100 9.40 9.08 9.04 10.6 10.38.25 9.05 6.55 8.85 9.03 1.19 100 3.74E−02 1.83 3.6 hsa-cand149hsa-cand149_st1 7.42 7.13 9.03 7.86 1.02 33 6.04 7.27 5.60 6.09 6.846.52 3.23 6.30 6.41 6.03 1.15 0 3.56E−02 1.83 3.6 hsa-asg-5829hsa-asg-5829_st1 5.99 6.40 5.52 5.97 0.44 33 5.18 3.96 5.01 4.58 4.424.10 2.32 4.25 3.46 4.14 0.86 0 6.27E−03 1.83 3.5 hsa-asg-922hsa-asg-922_st1 8.04 8.81 8.01 8.29 0.45 100 7.77 7.24 6.28 7.01 5.926.62 4.98 5.81 6.89 6.50 0.85 89 6.72E−03 1.78 3.4 hsa-asg-13127hsa-asg-13127_st2 7.86 8.69 8.25 8.27 0.41 100 7.75 7.36 5.86 6.68 6.056.84 5.66 5.08 7.13 6.49 0.88 67 8.10E−03 1.78 3.4 hsa-asg-8616hsa-asg-8616_st2 5.48 4.11 6.02 5.20 0.98 67 3.45 4.49 1.66 4.55 3.594.24 3.85 2.08 3.09 3.44 1.02 0 2.62E−02 1.76 3.4 hsa-miR-135bhsa-miR-135b_st2 3.66 5.86 3.52 4.35 1.32 33 1.45 2.71 2.43 2.85 2.373.13 3.29 2.27 2.92 2.60 0.55 0 6.77E−03 1.75 3.4 hsa-miR-135bhsa-cand78_st1 4.42 5.89 4.56 4.96 0.81 33 3.45 2.54 3.67 4.15 3.72 2.853.59 1.68 3.25 3.21 0.75 0 6.41E−03 1.74 3.3 hsa-asg- hsa-asg-10679_st24.14 6.26 5.23 5.21 1.06 33 4.09 3.02 3.67 4.76 3.39 2.69 3.17 3.32 3.093.47 0.63 0 5.22E−03 1.74 3.3 10679 hsa-miR-489 hsa-miR-489_st2 6.707.82 9.30 7.94 1.30 100 7.35 6.80 6.01 7.48 6.36 5.50 5.73 5.42 5.236.21 0.84 56 2.10E−02 1.73 3.3 hsa-miR-181d hsa-miR-181d_st1 9.83 10.310.0 10.0 0.24 100 9.13 7.83 8.91 8.93 7.59 7.96 8.28 8.21 8.02 8.320.54 100 3.96E−04 1.73 3.3 hsa-miR-181d hsa-asg-13508_st1 7.23 7.68 7.527.48 0.23 100 6.61 5.82 6.85 6.61 5.63 5.68 5.69 5.81 5.53 6.02 0.51 679.00E−04 1.45 2.7 hsa-miR-181d hsa-miR74_st1 9.52 10.0 9.80 9.78 0.26100 8.97 8.22 8.99 8.80 8.02 8.25 8.10 8.17 7.88 8.38 0.42 100 3.44E−041.41 2.7 hsa-asg- hsa-asg-13230_st2 7.55 8.09 7.99 7.88 0.28 100 7.466.04 7.10 7.36 4.72 5.57 6.22 7.09 3.77 6.15 1.28 78 4.77E−02 1.73 3.313230 hsa-miR-376a hsa-miR-376a_st2 5.76 6.55 5.90 6.07 0.42 100 5.144.08 5.49 5.67 4.85 4.03 2.48 3.83 3.77 4.37 1.01 0 2.01E−02 1.70 3.3hsa-miR-376a hsa-asg-10049_st1 5.32 7.02 6.40 6.25 0.86 100 5.03 4.916.09 5.56 5.35 4.10 3.85 4.14 4.67 4.85 0.74 11 2.14E−02 1.39 2.6hsa-asg- hsa-asg-14048_st1 6.18 5.93 5.87 5.99 0.16 100 4.43 4.95 3.543.63 5.03 3.26 4.59 5.15 4.07 4.29 0.70 0 2.43E−03 1.70 3.2 14048hsa-asg- hsa-asg-12690_st1 3.95 4.79 5.33 4.69 0.69 33 3.76 2.87 2.663.40 3.49 2.85 2.92 2.93 2.10 3.00 0.49 0 8.39E−04 1.69 3.2 12690hsa-miR-331 hsa-asg-14205_st1 8.00 8.37 8.06 8.14 0.20 100 7.53 5.857.37 7.77 6.85 4.77 6.64 5.46 5.93 6.47 1.02 56 2.10E−02 1.68 3.2hsa-miR-331 hsa-miR-331_st2 9.13 9.74 9.49 9.46 0.31 100 9.04 7.57 8.358.79 7.69 7.09 7.70 7.28 7.20 7.86 0.71 100 4.06E−03 1.60 3.0hsa-miR-432 hsa-miR-432_st2 6.55 6.33 6.61 6.50 0.15 100 4.55 5.43 5.925.53 5.77 5.68 3.50 4.28 2.74 4.82 1.13 22 3.24E−02 1.67 3.2 hsa-asg-hsa-asg-13661_st1 7.01 5.86 7.31 6.73 0.76 100 5.65 5.51 3.31 4.93 5.495.24 4.78 5.31 5.29 5.05 0.71 0 5.92E−03 1.67 3.2 13661 hsa-asg-4283hsa-asg-4283_st2 4.37 4.74 5.58 4.90 0.62 33 3.93 3.53 2.66 3.82 3.932.85 3.50 2.27 2.54 3.22 0.65 0 3.01E−03 1.67 3.2 hsa-asg-1619hsa-asg-1619_st2 4.65 5.10 5.26 5.00 0.32 33 4.37 3.29 2.86 4.81 3.162.85 2.32 3.07 3.25 3.33 0.78 0 5.53E−03 1.67 3.2 hsa-asg-4392hsa-asg-4392_st1 12.0 10.4 11.4 11.2 0.81 100 10.6 10.0 8.14 10.0 10.99.89 9.20 10.1 7.56 9.62 1.12 100 4.17E−02 1.66 3.2 hsa-asg-924hsa-asg-924_st1 4.20 6.46 4.52 5.06 1.22 33 4.66 4.30 3.39 4.46 2.692.77 2.15 2.78 3.53 3.41 0.89 0 2.88E−02 1.65 3.1 hsa-asg-8902hsa-asg-8902_st2 7.53 5.22 6.48 6.41 1.16 67 6.30 5.78 4.26 4.26 5.125.09 4.54 4.25 3.32 4.77 0.90 22 2.83E−02 1.64 3.1 hsa-miR-210hsa-miR-210_st1 7.96 8.71 8.00 8.23 0.42 100 6.51 5.65 6.84 7.08 7.116.71 6.11 6.48 7.02 6.61 0.49 89 4.66E−04 1.61 3.1 hsa-asg-548hsa-asg-548_st1 4.26 5.18 4.60 4.68 0.47 33 3.07 1.96 3.54 3.52 2.831.97 3.40 2.78 4.60 3.07 0.83 0 1.05E−02 1.61 3.0 hsa-asg-hsa-asg-11443_st1 2.98 6.08 5.12 4.73 1.59 67 4.17 3.64 2.86 2.69 3.542.69 3.40 2.78 2.33 3.12 0.59 0 2.17E−02 1.60 3.0 11443 hsa-miR-526chsa-miR-526c_st1 5.59 4.84 3.16 4.53 1.25 33 3.56 2.71 5.04 2.69 1.552.99 3.17 2.62 2.10 2.94 0.98 0 4.45E−02 1.59 3.0 hsa-miR-18bhsa-miR-18b_st1 6.45 8.39 6.72 7.19 1.05 100 6.18 5.61 6.71 6.01 5.094.68 5.71 4.87 5.60 5.61 0.65 44 9.98E−03 1.58 3.0 hsa-asg-6277hsa-asg-6277_st2 8.24 6.26 8.31 7.60 1.17 100 6.89 6.98 5.06 5.95 6.497.01 4.94 6.34 4.57 6.03 0.95 67 3.88E−02 1.58 3.0 hsa-asg-6292hsa-asg-6292_st2 11.1 9.17 10.6 10.3 1.04 100 9.77 9.62 7.44 8.84 9.908.86 7.99 9.27 7.11 8.76 1.03 67 4.52E−02 1.57 3.0 hsa-asg-1341hsa-asg-1341_st1 12.1 10.5 11.5 11.4 0.82 100 10.8 10.5 8.34 10.2 10.810.2 9.35 10.0 8.07 9.85 1.04 89 4.06E−02 1.57 3.0 hsa-asg-hsa-asg-13166_st2 5.37 5.74 5.47 5.53 0.19 100 4.49 3.42 4.87 4.95 4.114.18 3.59 2.08 4.07 3.97 0.88 0 1.42E−02 1.56 2.9 13166 hsa-miR-132hsa-miR-132_st1 9.99 10.01 9.64 9.88 0.21 100 9.72 8.56 8.33 8.35 8.598.08 8.02 8.44 6.88 8.33 0.74 89 5.76E−03 1.55 2.9 hsa-asghsa-asg-14146_st1 6.68 8.06 7.42 7.39 0.69 100 6.30 5.77 6.23 6.49 5.954.84 5.80 4.68 6.48 5.84 0.67 78 6.13E−03 1.55 2.9 14146 hsa-miR-181bhsa-miR-181b_st2 11.8 12.2 12.0 12.0 0.22 100 11.2 10.4 10.9 10.8 9.8310.3 10.2 10.4 10.1 10.5 0.44 100 2.02E−04 1.54 2.9 hsa-asg-hsa-asg-11850_st1 12.1 10.4 11.7 11.4 0.92 100 10.6 10.2 8.54 10.4 11.210.0 9.59 10.4 8.02 9.90 1.03 100 4.57E−02 1.53 2.9 11850 hsa-miR-522hsa-miR-522_st2 5.37 4.52 3.95 4.61 0.72 33 2.78 2.87 3.67 3.15 2.973.48 2.48 3.44 2.92 3.08 0.38 0 6.36E−04 1.53 2.9 hsa-asg-2535hsa-asg-2535_st2 9.22 7.62 8.76 8.53 0.82 100 8.10 7.49 5.53 7.17 7.787.18 6.38 7.87 5.66 7.02 0.95 33 3.39E−02 1.51 2.9 hsa-asg-1203hsa-asg-1203_st1 4.99 4.89 5.87 5.25 0.54 33 4.49 4.37 2.66 3.82 4.214.55 3.93 3.74 1.86 3.74 0.91 0 2.31E−02 1.51 2.9 hsa-miR-335hsa-miR-335_st1 10.3 11.5 10.8 10.9 0.57 100 10.9 10.3 8.69 10.0 9.479.34 9.58 7.33 9.03 9.42 1.03 100 4.03E−02 1.50 2.8 hsa-miR-30e-hsa-miR-30e- 10.7 11.6 10.8 11.0 0.49 100 10.4 9.64 9.71 10.5 9.81 9.239.23 8.15 9.50 9.58 0.70 100 6.98E−03 1.50 2.8 5p 5p_st2 hsa-cand283hsa-cand283_st1 6.92 6.20 6.85 6.66 0.40 100 5.45 6.14 4.68 5.56 4.745.63 5.25 4.58 4.54 5.17 0.57 33 1.97E−03 1.48 2.8 hsa-asg-5210hsa-asg-5210_st1 10.7 9.62 10.3 10.2 0.55 100 9.63 8.99 7.52 9.06 9.738.45 8.37 9.19 7.69 8.74 0.79 100 1.38E−02 1.48 2.8 hsa- hsa-miR144_st23.49 6.16 5.00 4.88 1.34 67 4.61 4.43 2.66 3.40 2.69 2.69 2.78 3.20 4.243.41 0.81 0 4.03E−02 1.47 2.8 miR144_st2 hsa-miR-148b hsa-miR-148b_st210.1 10.8 11.0 10.6 0.47 100 10.0 8.79 9.44 10.0 9.63 8.27 9.09 8.149.14 9.18 0.70 100 7.32E−03 1.47 2.8 hsa-miR-612 hsa-miR-612_st1 7.046.08 7.21 6.78 0.61 100 5.39 6.49 4.72 4.19 4.93 6.34 3.93 5.58 6.215.31 0.94 33 3.12E−02 1.47 2.8 hsa-miR-454- hsa-asg-11541_st2 8.57 10.49.58 9.52 0.92 100 8.63 8.31 8.53 8.45 7.87 7.92 7.91 7.33 7.81 8.080.42 100 3.21E−03 1.44 2.7 3p hsa-miR-454- hsa-asg-13305_st1 9.36 10.910.1 10.1 0.77 100 9.63 8.98 9.24 9.13 8.55 8.77 8.57 7.82 8.33 8.780.54 100 6.62E−03 1.35 2.5 3p hsa-asg- hsa-asg-13471_st1 5.91 5.98 6.316.07 0.21 100 4.76 4.76 4.39 5.12 5.35 5.40 4.38 4.07 3.53 4.64 0.61 113.26E−03 1.43 2.7 13471 hsa-asg-4294 hsa-asg-4294_st2 4.81 5.10 5.064.99 0.16 33 4.31 3.16 4.54 4.08 3.22 3.13 2.32 4.21 3.09 3.56 0.74 09.55E−03 1.43 2.7 hsa-asg- hsa-asg-12880_st1 8.90 7.40 8.65 8.32 0.81100 8.02 7.00 5.74 6.88 7.99 7.03 6.49 7.25 5.69 6.90 0.83 33 2.80E−021.42 2.7 12880 hsa-asg-109 hsa-asg-109_st1 9.45 8.18 9.38 9.00 0.71 1008.15 8.15 5.74 7.66 8.37 7.69 7.27 8.33 6.92 7.59 0.85 89 2.77E−02 1.412.7 hsa-asg- hsa-asg-12271_st1 5.92 4.11 6.13 5.39 1.11 67 3.85 4.863.79 3.57 3.59 4.93 3.17 4.07 3.97 3.98 0.58 0 1.47E−02 1.41 2.7 12271hsa-asg- hsa-asg-10638_st2 10.6 9.04 10.7 10.1 0.95 100 9.25 9.25 7.428.69 9.25 9.08 8.33 8.71 8.53 8.72 0.60 100 1.11E−02 1.41 2.7 10638hsa-miR-130a hsa-miR-130a_st1 10.8 12.0 11.6 11.5 0.61 100 10.3 9.969.71 10.9 10.7 9.21 10.0 9.63 10.4 10.1 0.56 100 4.32E−03 1.40 2.6hsa-miR-130a hsa-cand144_st1 10.8 12.0 11.6 11.5 0.58 100 10.4 9.85 9.7210.9 10.8 9.26 10.0 9.56 10.6 10.1 0.59 100 6.07E−03 1.36 2.6hsa-asg-3267 hsa-asg-3267_st1 9.11 7.48 9.41 8.67 1.04 33 7.73 7.84 6.326.77 8.08 7.63 6.68 7.78 6.72 7.29 0.65 0 1.91E−02 1.38 2.6 hsa-asg-1380hsa-asg-1380_st2 5.15 5.02 4.76 4.98 0.20 67 4.09 3.42 1.66 4.08 4.043.95 3.40 4.41 3.39 3.60 0.82 0 1.89E−02 1.37 2.6 hsa-as-5241hsa-asg-5241_st1 4.14 4.79 4.76 4.56 0.36 33 3.66 3.64 2.66 3.28 3.222.53 3.59 2.45 3.77 3.20 0.52 0 2.05E−03 1.37 2.6 hsa-asg-3711hsa-asg-3711_st2 6.53 6.73 6.36 6.54 0.19 67 5.70 6.17 5.12 5.53 5.145.25 4.54 4.44 4.87 5.19 0.55 0 2.40E−03 1.35 2.5 hsa-asg-60hsa-asg-60_st2 4.31 4.26 4.83 4.47 0.31 33 3.56 4.23 3.79 2.85 2.97 2.851.08 3.74 3.25 3.15 0.91 0 3.72E−02 1.32 2.5 hsa-asg- hsa-asg-12515_st24.97 4.93 6.07 5.32 0.65 33 4.02 4.30 3.06 4.12 4.60 4.37 3.68 4.14 3.774.01 0.46 0 2.74E−03 1.32 2.5 12515 hsa-miR-340 hsa-miR-340_st1 5.436.58 6.06 6.02 0.58 67 5.83 4.99 5.24 5.71 4.89 4.46 4.49 3.99 2.74 4.710.94 11 4.91E−02 1.32 2.5 hsa-asg-2315 hsa-asg-2315_st2 6.92 4.98 6.486.12 1.02 33 5.60 5.83 4.58 4.66 5.39 4.91 3.68 4.85 3.92 4.82 0.72 03.28E−02 1.30 2.5 hsa-cand330 hsa-cand330_st2 4.95 5.29 6.31 5.52 0.71100 4.76 5.04 3.54 4.71 5.15 4.18 2.64 3.99 3.97 4.22 0.80 11 3.24E−021.30 2.5 hsa-asg-6203 hsa-asg-6203_st2 9.37 7.41 8.68 8.48 1.00 100 7.897.78 6.94 7.02 7.43 7.51 6.49 7.43 6.26 7.19 0.56 100 1.62E−02 1.29 2.4hsa-miR-99b hsa-miR-99b_st1 11.6 11.8 11.9 11.8 0.15 100 11.0 10.5 10.511.4 11.1 10.1 10.1 9.52 10.3 10.5 0.59 100 4.72E−03 1.29 2.4hsa-miR-99b hsa-asg-14196_st2 11.7 11.9 12.0 11.9 0.15 100 11.2 10.610.6 11.6 11.3 10.3 10.3 9.55 10.5 10.7 0.65 100 9.51E−03 1.24 2.4hsa-asg- hsa-asg-11521_st1 8.14 7.12 8.39 7.89 0.68 100 7.13 7.83 5.556.35 7.20 7.38 5.69 6.42 5.88 6.60 0.82 89 3.55E−02 1.28 2.4 11521hsa-asg-9053 hsa-asg-9053_st1 7.50 6.46 7.50 7.15 0.60 100 5.87 6.554.78 6.21 6.10 6.92 4.90 5.92 5.64 5.88 0.70 33 1.83E−02 1.28 2.4hsa-asg-7471 hsa-asg-7471_st1 6.18 7.20 7.49 6.95 0.69 100 5.60 5.725.67 6.74 5.70 5.30 5.25 5.00 6.09 5.68 0.51 56 5.99E−03 1.28 2.4hsa-asg-5326 hsa-asg-5326_st2 8.42 6.33 7.96 7.57 1.10 100 6.96 7.185.74 6.27 6.67 6.52 5.54 6.57 5.35 6.31 0.64 67 3.11E−02 1.26 2.4hsa-miR-652 hsa-miR-652_st1 10.3 11.3 10.4 10.7 0.53 100 9.78 9.28 9.209.98 9.13 9.06 9.64 9.52 9.66 9.47 0.32 100 4.76E−04 1.26 2.4 hsa-asg-67hsa-asg-67_st2 4.46 4.74 5.17 4.79 0.36 33 4.55 3.29 4.02 2.33 3.22 3.593.68 3.54 3.65 3.54 0.60 0 7.56E−03 1.25 2.4 hsa-miR-511 hsa-miR-511_st27.00 7.60 6.98 7.19 0.35 100 5.78 5.52 6.30 6.46 6.34 5.80 5.90 6.115.39 5.95 0.37 89 5.16E−04 1.24 2.4 hsa-asg-7823 hsa-asg-7823_st1 8.637.23 8.71 8.19 0.83 100 7.36 7.93 6.27 6.86 7.29 7.72 6.18 6.58 6.436.96 0.64 100 2.24E−02 1.23 2.3 hsa-miR-566 hsa-miR-566_st1 8.13 5.717.54 7.13 1.26 100 6.73 6.17 5.12 5.83 6.14 6.15 5.56 6.04 5.44 5.910.48 56 2.72E−02 1.22 2.3 hsa-asg-925 hsa-asg-925_st1 6.56 8.50 7.587.55 0.97 100 7.36 6.37 6.19 6.97 6.35 5.27 5.82 5.74 6.88 6.33 0.66 893.25E−02 1.22 2.3 hsa-miR-181c hsa-miR-181c_st1 9.65 10.5 10.1 10.1 0.47100 9.25 8.62 9.33 9.10 8.67 7.71 9.06 8.70 9.83 8.92 0.59 100 9.63E−031.21 2.3 hsa-asg-1221 hsa-asg-1221_st2 4.65 5.18 4.43 4.75 0.39 33 4.023.29 4.39 4.23 3.77 3.13 2.64 2.93 3.53 3.55 0.60 0 9.37E−03 1.21 2.3hsa-asg-5115 hsa-asg-5115_st1 8.57 7.74 8.80 8.37 0.56 100 6.98 8.056.75 7.16 6.99 7.39 6.19 7.54 7.46 7.17 0.53 100 7.31E−03 1.20 2.3hsa-cand173 hsa-cand173_st1 8.25 6.06 7.55 7.29 1.11 100 6.75 7.27 5.915.65 6.24 6.29 5.05 6.14 5.58 6.10 0.66 56 4.33E−02 1.19 2.3hsa-miR-30a- hsa-miR-30a- 9.88 9.19 10.1 9.74 0.49 100 9.33 9.03 8.269.31 8.40 9.13 7.93 7.39 8.19 8.55 0.68 100 2.08E−02 1.19 2.3 3p 3p_st1hsa-asg-954 hsa-asg-954_st1 9.94 8.92 9.85 9.57 0.56 100 8.99 8.81 8.098.96 8.79 8.43 8.04 7.94 7.51 8.40 0.53 100 7.95E−03 1.17 2.3Has-miR-151 hsa-miR-151_st2 10.1 10.7 10.7 10.5 0.34 100 10.2 9.76 9.149.80 9.38 9.47 9.04 8.51 9.05 9.37 0.50 100 4.32E−03 1.16 2.2Has-miR-10a hsa-miR-10a_st1 12.1 12.4 12.2 12.2 0.13 100 12.0 11.0 11.211.7 11.3 10.5 11.2 9.98 10.9 11.1 0.61 100 9.71E−03 1.16 2.2Has-asg-13606 hsa-asg-13606_st2 11.1 9.67 10.3 10.3 0.72 100 10.4 9.698.35 9.15 9.77 9.22 8.72 9.63 7.86 9.20 0.79 100 4.93E−02 1.16 2.2hsa-asg-14233 hsa-asg-14233_st1 10.1 10.5 10.5 10.3 0.22 100 9.45 9.269.23 10.1 9.44 8.89 9.05 8.87 8.89 9.25 0.41 100 1.05E−03 1.14 2.2hsa-miR-660 hsa-miR-660_st1 9.32 10.2 9.42 9.67 0.52 100 9.29 8.99 7.939.15 8.63 8.44 7.79 7.91 8.61 8.53 0.56 100 1.09E−02 1.14 2.2hsa-miR-222 hsa-miR-222_st2 10.9 10.8 10.8 10.8 0.08 100 10.4 9.56 9.5410.2 9.75 9.33 9.52 9.38 9.86 9.73 0.37 100 4.45E−04 1.14 2.2hsa-miR-19b hsa-miR-19b_st2 7.92 9.53 8.38 8.61 0.83 100 8.38 8.32 7.318.03 8.04 6.51 6.91 6.53 7.20 7.47 0.74 100 4.81E−02 1.14 2.2hsa-asg-6758 hsa-asg-6758_st1 4.51 5.32 4.72 4.85 0.42 33 4.17 2.87 3.394.55 4.39 2.69 4.14 2.93 4.32 3.72 0.74 0 3.28E−02 1.14 2.2 hsa-miR-106bhsa-miR-106b_st2 9.48 11.6 10.7 10.6 1.07 100 9.91 9.64 10.0 10.0 9.438.17 9.21 9.10 9.76 9.48 0.59 100 3.88E−02 1.14 2.2 hsa-miR-324-hsa-miR-324- 8.20 9.93 9.05 9.06 0.86 100 7.92 8.13 8.33 8.27 7.80 7.427.82 7.87 7.98 7.95 0.27 100 4.51E−03 1.11 2.2 5p 5p_st1 hsa-asg-11877hsa-asg-11877_st1 5.82 5.32 6.80 5.98 0.75 33 4.37 5.71 5.21 4.49 5.365.03 3.85 4.89 5.07 4.89 0.57 0 2.21E−02 1.09 2.1 hsa-asg-30hsa-asg-30_st1 12.4 11.2 11.9 11.8 0.61 100 11.5 11.2 9.95 10.6 11.410.9 10.3 11.0 9.79 10.7 0.63 100 2.56E−02 1.09 2.1 hsa-asg-9004hsa-asg-9004_st1 8.82 8.38 9.78 8.99 0.72 100 7.58 8.47 6.90 7.20 8.508.08 7.36 8.60 8.44 7.90 0.65 100 3.37E−02 1.09 2.1 hsa-asg-191hsa-asg-191_st1 4.17 5.06 5.24 4.83 0.57 33 3.33 3.92 2.86 3.57 3.854.10 4.00 3.20 4.84 3.74 0.58 0 1.88E−02 1.08 2.1 hsa-asg-12421hsa-asg-12421_st1 9.22 7.49 8.51 8.41 0.87 67 8.10 7.93 6.72 7.33 7.727.49 6.61 7.79 6.39 7.34 0.62 44 4.08E−02 1.07 2.1 hsa-miR257hsa-miR257_st1 9.43 10.5 9.72 9.89 0.57 100 9.62 9.10 9.23 9.55 8.878.54 8.50 8.15 7.91 8.83 0.60 100 2.28E−02 1.06 2.1 hsa-miR-30a-hsa-miR-30a- 12.6 13.2 12.8 12.9 0.32 100 12.2 12.1 11.9 12.5 12.1 11.711.5 10.8 11.8 11.8 0.48 100 5.34E−03 1.06 2.1 5p 5p_st2 hsa-asg-9117hsa-asg-9117_st1 7.20 5.71 7.06 6.66 0.82 67 5.51 6.42 5.21 5.53 5.366.33 5.40 4.71 5.91 5.60 0.54 0 2.57E−02 1.06 2.1 hsa-miR-30dhsa-miR-30d_st1 13.2 13.7 13.2 13.3 0.32 100 12.7 12.1 12.4 12.9 12.411.8 12.1 11.6 12.5 12.3 0.43 100 3.27E−03 1.05 2.1 hsa-asg-3251hsa-asg-3251_st1 5.78 4.26 3.75 4.60 1.06 33 4.24 4.16 4.02 2.85 3.543.95 2.92 3.07 3.25 3.56 0.55 0 4.53E−02 1.04 2.1 hsa-miR-134hsa-miR-134_st2 5.55 6.62 6.52 6.23 0.59 33 5.11 5.78 5.99 5.85 5.704.21 4.73 4.31 5.02 5.19 0.68 0 3.99E−02 1.04 2.1 hsa-asg-2495hsa-asg-2495_st2 5.57 4.79 5.33 5.23 0.40 67 4.76 4.37 3.23 3.73 4.724.03 4.54 5.26 3.09 4.19 0.73 0 4.41E−02 1.04 2.1 hsa-miR-27ahsa-miR-27a_st2 11.5 12.5 12.2 12.1 0.46 100 11.3 11.0 11.0 12.0 11.510.6 10.6 10.2 11.1 11.0 0.54 100 1.52E−02 1.03 2.0 hsa-asg-5032hsa-asg-5032_st2 9.60 8.30 9.19 9.03 0.66 100 8.74 8.73 7.36 7.60 8.438.35 7.14 7.72 7.95 8.00 0.59 100 2.89E−02 1.03 2.0 hsa-asg-6618hsa-asg-6618_st2 6.89 5.32 7.02 6.41 0.95 67 5.48 5.99 4.50 5.32 5.746.11 4.94 5.20 5.25 5.39 0.51 22 3.33E−02 1.02 2.0 hsa-miR-382hsa-miR-382_st1 7.78 7.65 7.76 7.73 0.07 100 7.17 6.85 7.35 7.13 7.436.56 6.29 5.93 5.81 6.72 0.61 78 1.98E−02 1.01 2.0 hsa-asg-3145hsa-asg-3145_st1 6.47 6.36 6.56 6.46 0.10 100 5.68 5.19 5.28 6.40 6.255.59 4.98 4.47 5.35 5.46 0.60 33 1.98E−02 1.00 2.0 SD, standarddeviation; %, percentage of samples expressing miRNA above backgroundlevels; Log2Diff, difference in VSN-transformed expression bewteen LUNand LNneg samples.

Example 26 miRNAs Differentally Expressed Between Normal Pancreas Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous pancreas cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal pancreastissue than in cancer-negative lymph nodes.

Total RNA, purified from two separate normal human pancreas tissuesamples (FirstChoice® Human Pancreas Total RNA, cat. no. AM7954 Ambion,Austin, Tex., USA) and from nine normal human lymph node samples(FirstChoice® Human Lymph Node Total RNA, cat. no. AM7894; Ambion,Austin, Tex., USA) was purchased from Ambion. miRNA purification andlabeling and microarray expression analyses were performed as describedabove in Example 2.

The inventors identified 195 miRNAs (62 verified, 133 predicted) withsignificant differential expression between normal pancreas and normallymph node samples samples (|log 2Diff| pancreas vs LN neg≧1 andp-value<0.05) (Table 25). Among the predicted miRNAs, hsa-miR-375 and-217 had average expression levels over 800-fold higher in the pancreassamples; hsa-miR-216 and -429 had average expression levels that werebetween 100- and 200-fold higher in pancreas; hsa-miR-200a, -141 and-200b had average expression levels that were between 50- and 75-foldhigher in pancreas; hsa-miR-200c, -96, -605, and -802) had averageexpression levels that were between 20- and 40-fold higher in pancreas;15 miRNAs had average expression levels that were between 10- and20-fold higher in pancreas; and 20 miRNAs had average expression levelsthat were between 5- and 10-fold higher in pancreas samples than innormal lymph nodes. Among the predicted miRNAs, hsa-asg-7615_st2,-4913_st1, -14153_st2, and -5618_st1 had average expression levels thatwere between 50- and 80-fold higher in pancreas samples; 24 miRNA hadaverage expression levels that were between 10- and 40-fold higher inpancreas; and 57 miRNAs had average expression levels that were between5- and 10-fold higher in pancreas samples than in normal lymph nodes.

TABLE 25 MicroRNAs Differentially Expressed Between Normal PancreasSamples (PAN) and Cancer-Negative Lymph Node Samples (LNneg) fromPatients. PAN LNneg Micro RNA Probe ID 1 2 M SD % 1 2 3 4 5 6 7 8 9 M SD% ttest log2Diff Fold hsa-miR-375 hsa-miR-375_st1 14.2 14.1 14.1 0.04100 5.96 4.93 6.23 2.85 2.97 3.69 0.99 4.63 6.39 4.29 1.82 0 3.78E−06−9.87 935. hsa-miR-217 hsa-miR-217_st1 12.8 12.6 12.8 0.19 100 3.33 3.642.66 2.51 3.81 3.37 2.78 2.93 2.74 3.09 0.46 0 9.27E−12 −9.78 882.hsa-miR-216 hsa-miR-216_st2 12.2 11.7 12.1 0.34 100 4.49 3.92 5.04 4.844.72 4.87 4.33 5.04 4.78 4.67 0.37 0 2.70E−11 −7.47 177. hsa-miR-429hsa-miR-429_st1 10.4 9.25 9.86 0.62 100 3.45 1.54 3.67 2.13 2.37 2.692.32 2.45 5.39 2.89 1.14 0 1.72E−06 −6.97 125. hsa-asg-7615hsa-asg-7615_st2 8.70 8.69 8.60 0.17 100 2.25 1.96 2.66 2.23 2.19 1.882.78 1.88 3.09 2.32 0.43 0 3.55E−10 −6.27 77.3 hsa-miR-200ahsa-miR-200a_st2 12.1 11.3 11.7 0.40 100 6.27 5.08 6.49 5.72 5.29 4.604.07 4.78 7.78 5.56 1.14 67 4.17E−06 −6.20 73.5 hsa-asg-4913hsa-asg-4913_st1 8.30 7.51 8.05 0.47 100 2.05 1.96 1.66 2.77 2.01 1.881.60 2.45 1.38 1.97 0.43 0 1.43E−09 −6.07 67.3 hsa-asg-14153hsa-asg-14153_st2 8.98 8.54 8.80 0.23 100 3.07 2.26 3.06 3.01 2.37 2.691.16 2.78 4.40 2.75 0.86 0 3.68E−07 −6.04 65.9 hsa-miR-141hsa-miR-141_st1 10.7 9.26 10.0 0.75 100 5.07 3.83 4.93 4.43 4.39 2.853.85 3.83 4.54 4.19 0.68 0 1.72E−07 −5.88 59.0 hsa-miR-200bhsa-miR-200b_st1 13.6 13.3 13.4 0.18 100 7.62 7.80 8.90 6.69 6.82 6.876.51 7.65 10.4 7.70 1.26 100 1.76E−05 −5.77 54.6 hsa-asg-5618hsa-asg-5618_st1 7.51 6.95 7.29 0.30 100 1.65 1.14 1.14 1.41 1.37 2.351.97 1.48 1.86 1.60 0.40 0 7.59E−10 −5.70 51.9 hsa-miR-200chsa-miR-200c_st1 14.4 14.1 14.3 0.14 100 9.60 8.84 10.3 8.18 8.15 8.147.98 8.89 11.5 9.07 1.21 100 2.69E−05 −5.24 37.8 hsa-asg-7637hsa-asg-7637_st2 9.08 8.97 9.01 0.06 100 3.66 2.54 4.47 3.46 3.93 4.033.29 3.64 5.27 3.81 0.76 0 4.68E−07 −5.20 36.9 hsa-miR-96 hsa-miR-96_st18.47 7.76 8.35 0.55 100 4.99 3.42 3.06 3.01 4.11 2.85 3.05 1.88 3.093.27 0.86 11 2.78E−06 −5.08 33.8 hsa-miR-605 hsa-miR-605_st1 7.19 7.277.15 0.14 100 2.61 2.16 3.06 0.75 1.74 2.17 2.92 3.07 4.47 2.55 1.03 02.18E−05 −4.61 24.3 hsa-miR-802 hsa-asg-353_st1 6.23 6.55 6.47 0.21 1002.43 3.02 2.66 2.51 2.53 0.84 0.62 2.62 1.86 2.12 0.85 0 6.58E−06 −4.3520.4 hsa-miR-802 hsa-miR140_st1 4.82 5.31 5.40 0.63 33 1.25 2.36 1.391.51 1.83 1.78 0.76 3.07 1.62 1.73 0.66 0 7.93E−06 −3.67 12.8hsa-asg-9219 hsa-asg-9219_st2 6.03 6.50 6.27 0.24 67 2.61 1.75 2.18 2.601.83 1.88 1.42 1.29 2.33 1.99 0.48 0 4.47E−08 −4.28 19.4 hsa-miR-493-5phsa-miR-493-5p_st1 9.33 8.69 8.94 0.34 100 4.37 3.83 6.88 6.18 6.33 3.694.38 1.88 4.60 4.68 1.56 33 1.08E−03 −4.25 19.1 hsa-miR-215hsa-miR-215_st1 10.2 9.55 9.86 0.39 100 6.96 5.82 5.71 6.66 5.54 4.845.51 5.11 5.23 5.71 0.70 33 2.27E−06 −4.15 17.8 hsa-asg-13404hsa-asg-13404_st2 7.13 6.91 6.98 0.13 100 4.17 2.71 5.28 2.93 3.28 1.582.15 1.11 2.33 2.84 1.29 0 3.09E−04 −4.14 17.7 hsa-miR-411hsa-miR-411_st1 8.05 7.77 7.95 0.16 100 4.85 3.74 4.54 5.23 5.56 2.993.40 2.27 2.10 3.85 1.27 11 2.93E−04 −4.10 17.2 hsa-asg-11715hsa-asg-11715_st2 5.66 6.77 5.98 0.69 67 1.85 2.36 2.18 1.51 2.11 1.780.62 1.48 3.39 1.92 0.75 0 9.42E−06 −4.06 16.6 hsa-asg-13908hsa-asg-13908_st2 6.91 6.66 6.65 0.26 100 2.05 2.54 3.67 2.51 1.46 2.852.64 2.45 3.77 2.66 0.72 0 3.61E−06 −3.99 15.9 hsa-miR-487bhsa-miR-487b_st2 9.89 9.13 9.57 0.40 100 6.06 5.84 6.97 6.90 6.25 5.404.90 3.74 4.40 5.61 1.10 67 1.44E−04 −3.96 15.6 hsa-asg-10131hsa-asg-10131_st2 7.53 7.42 7.16 0.55 100 3.33 3.16 3.54 2.51 2.53 3.262.64 3.20 5.09 3.25 0.78 0 1.35E−05 −3.91 15.0 hsa-miR-183hsa-miR-183_st1 11.5 10.3 10.8 0.61 100 7.66 6.76 6.71 7.93 7.56 5.837.52 6.32 6.65 6.99 0.70 89 7.14E−06 −3.88 14.7 hsa-asg-10247hsa-asg-10247_st1 6.52 6.39 6.40 0.11 100 2.78 2.16 4.02 1.72 2.19 2.350.35 3.20 3.97 2.53 1.14 0 2.05E−04 −3.88 14.7 hsa-miR-7 hsa-miR-7_st112.8 12.1 12.4 0.33 100 9.46 8.23 9.70 8.62 8.19 7.70 8.66 8.58 8.488.62 0.62 100 1.68E−06 −3.81 14.0 hsa-asg-6160 hsa-asg-6160_st1 5.635.90 5.80 0.15 33 3.45 1.75 2.18 2.77 2.11 1.01 1.25 1.11 2.33 1.99 0.810 1.39E−05 −3.81 14.0 hsa-asg-2381 hsa-asg-2381_st2 8.79 8.61 8.82 0.23100 6.35 4.81 6.11 6.00 4.42 5.17 4.20 4.83 3.25 5.02 1.01 56 9.35E−05−3.80 14.0 hsa-asg-12139 hsa-asg-12139_st2 5.32 6.59 6.12 0.69 67 2.782.36 2.66 2.33 1.64 2.17 1.60 2.08 3.53 2.35 0.59 0 3.45E−06 −3.77 13.6hsa-asg-13705 hsa-asg-13705_st1 6.09 6.26 6.05 0.24 100 1.65 0.95 3.792.33 2.69 2.35 0.35 3.20 3.25 2.29 1.12 0 2.34E−04 −3.76 13.6hsa-asg-1296 hsa-asg-1296_st1 7.08 7.44 6.99 0.51 100 3.07 2.54 4.812.42 2.53 2.77 3.17 3.91 4.16 3.27 0.84 0 3.28E−05 −3.72 13.2hsa-miR-192 hsa-miR-192_st2 13.4 13.1 13.3 0.21 100 10.8 9.87 10.1 10.09.58 8.72 9.33 8.80 9.65 9.66 0.66 100 3.15E−06 −3.71 13.1 hsa-miR-539hsa-miR-539_st1 6.78 5.72 6.21 0.54 67 2.05 3.16 2.66 3.40 2.69 2.991.97 2.62 1.38 2.55 0.64 0 4.81E−06 −3.67 12.7 hsa-miR-539hsa-miR177_st1 6.40 5.01 5.68 0.70 33 2.61 2.54 1.39 2.42 1.83 1.88 3.501.68 2.10 2.22 0.63 0 1.12E−05 −3.46 11.0 hsa-asg-14182hsa-asg-14182_st1 5.74 4.83 5.54 0.64 33 2.43 0.95 1.92 1.82 2.61 1.782.78 1.88 0.73 1.88 0.69 0 1.13E−05 −3.66 12.7 hsa-miR-368hsa-miR-368_st2 10.2 9.27 9.92 0.56 100 6.49 6.52 7.70 8.08 7.70 5.245.64 3.99 5.09 6.27 1.39 67 1.55E−03 −3.65 12.5 hsa-miR-338hsa-miR-338_st1 8.54 7.16 7.95 0.72 100 5.11 4.08 5.57 4.86 4.04 2.174.78 3.44 4.89 4.33 1.03 11 2.41E−04 −3.63 12.4 hsa-asg-5021hsa-asg-5021_st1 8.25 7.99 8.26 0.29 100 5.18 4.43 6.99 6.06 5.91 3.544.59 3.07 2.10 4.65 1.57 22 3.23E−03 −3.61 12.2 hsa-asg-11088hsa-asg-11088_st1 5.89 5.91 5.90 0.01 33 1.25 1.05 3.85 1.82 0.94 2.351.60 3.38 4.50 2.31 1.31 0 9.55E−04 −3.60 12.1 hsa-asg-7472hsa-asg-7472_st2 5.32 5.61 5.58 0.25 33 1.65 2.16 1.92 2.03 2.90 1.783.59 0.93 0.93 1.99 0.85 0 3.82E−05 −3.59 12.1 hsa-miR202 hsa-miR202_st27.35 6.57 7.07 0.43 100 3.85 2.71 4.93 3.95 3.63 2.44 4.27 2.45 3.093.48 0.87 0 5.07E−05 −3.59 12.0 hsa-miR-381 hsa-miR-381_st2 7.04 5.916.67 0.66 67 2.78 2.36 4.21 3.40 4.21 2.99 3.50 1.29 3.25 3.11 0.91 01.08E−04 −3.56 11.8 hsa-miR-154 hsa-miR-154_st2 7.54 6.60 7.03 0.48 1003.76 2.54 4.30 4.36 4.91 3.13 3.17 1.29 3.97 3.49 1.10 0 3.68E−04 −3.5411.6 hsa-cand852 hsa-cand852_st2 6.21 6.11 6.21 0.10 67 3.07 1.96 2.863.01 2.28 2.69 2.92 2.08 3.53 2.71 0.51 0 4.85E−07 −3.49 11.3hsa-asg-8128 hsa-asg-8128_st1 6.17 4.88 6.32 1.52 67 3.45 4.04 3.46 3.013.39 2.92 2.15 0.84 2.21 2.83 0.96 0 7.50E−04 −3.49 11.2 hsa-miR-410hsa-miR-410_st1 5.98 5.75 6.01 0.28 67 2.43 2.36 1.39 3.73 2.53 2.853.17 1.88 2.54 2.54 0.68 0 7.81E−06 −3.47 11.1 hsa-asg-3822hsa-asg-3822_st2 6.17 6.10 6.21 0.13 33 2.05 3.02 3.67 2.03 2.45 2.171.60 2.93 4.78 2.75 0.99 0 1.61E−04 −3.46 11.0 hsa-asg-5119hsa-asg-5119_st1 5.98 6.01 5.96 0.08 67 2.43 2.71 1.92 1.93 2.01 2.532.92 2.62 3.65 2.53 0.55 0 1.15E−06 −3.43 10.8 hsa-asg-8616hsa-asg-8616_st2 6.73 5.39 6.87 1.56 67 3.45 4.49 1.66 4.55 3.59 4.243.85 2.08 3.09 3.44 1.02 0 1.19E−03 −3.43 10.8 hsa-asg-10080hsa-asg-10080_st1 6.62 5.53 5.76 0.77 33 2.05 1.75 2.43 1.82 2.37 2.442.48 2.27 3.39 2.33 0.48 0 2.98E−06 −3.43 10.8 hsa-miR-182hsa-miR-182_st1 12.7 12.5 12.6 0.10 100 10.2 10.0 8.85 9.82 9.37 9.558.99 8.57 8.13 9.28 0.70 100 1.03E−05 −3.40 10.6 hsa-asg-9830hsa-asg-9830_st2 5.17 6.60 6.07 0.78 67 3.07 2.54 1.92 2.23 2.19 2.263.50 2.27 4.24 2.69 0.76 0 5.91E−05 −3.38 10.4 hsa-miR-432hsa-miR-432_st2 8.50 8.20 8.16 0.36 100 4.55 5.43 5.92 5.53 5.77 5.683.50 4.28 2.74 4.82 1.13 22 6.18E−04 −3.34 10.1 hsa-miR-495hsa-miR-495_st1 9.00 8.03 8.58 0.50 100 6.02 4.81 6.97 6.26 6.32 4.435.16 2.45 4.89 5.26 1.35 56 2.29E−03 −3.32 10.0 hsa-asg-1619hsa-asg-1619_st2 6.34 5.35 6.65 1.48 67 4.37 3.29 2.86 4.81 3.16 2.852.32 3.07 3.25 3.33 0.78 0 4.15E−04 −3.32 10.0 hsa-miR-376ahsa-miR-376a_st2 7.91 7.21 7.66 0.39 100 5.14 4.08 5.49 5.67 4.85 4.032.48 3.83 3.77 4.37 1.01 0 3.26E−04 −3.28 9.7 hsa-miR-376ahsa-asg-14234_st1 6.86 6.24 6.70 0.41 100 3.33 4.30 3.39 4.00 4.85 3.692.92 4.07 1.86 3.60 0.87 0 1.69E−04 −3.10 8.6 hsa-miR-376ahsa-asg-10049_st1 8.04 7.35 7.91 0.50 100 5.03 4.91 6.09 5.56 5.35 4.103.85 4.14 4.67 4.85 0.74 11 6.55E−05 −3.05 8.3 hsa-asg-10947hsa-asg-10947_st1 6.45 5.08 6.63 1.64 67 3.66 3.53 1.66 5.10 3.77 3.693.59 2.62 2.54 3.35 0.97 0 1.52E−03 −3.28 9.7 hsa-asg-4219hsa-asg-4219_st1 6.28 5.86 5.73 0.63 33 3.45 1.75 1.66 1.51 2.28 1.782.92 2.78 3.97 2.46 0.87 0 1.49E−04 −3.28 9.7 hsa-asg-13254hsa-asg-13254_st1 7.94 7.78 8.15 0.50 100 5.60 5.37 6.65 5.89 5.90 4.034.73 2.45 3.39 4.89 1.36 56 2.77E−03 −3.26 9.6 hsa-asg-1270hsa-asg-1270_st2 6.55 5.81 6.89 1.29 100 4.24 3.64 1.92 4.08 4.58 4.893.05 3.32 3.25 3.66 0.90 0 6.46E−04 −3.23 9.4 hsa-asg-3711hsa-asg-3711_st2 8.56 8.03 8.41 0.34 100 5.70 6.17 5.12 5.53 5.14 5.254.54 4.44 4.87 5.19 0.55 0 2.96E−06 −3.22 9.3 hsa-asg-12841hsa-asg-12841_st1 6.09 5.42 6.55 1.41 33 4.09 3.87 2.18 4.15 4.04 4.102.71 3.20 1.62 3.33 0.96 0 1.07E−03 −3.22 9.3 hsa-asg-5617hsa-asg-5617_st1 6.15 5.69 6.00 0.27 67 3.76 3.29 3.91 3.63 3.44 2.691.42 1.48 1.62 2.80 1.03 0 4.30E−04 −3.20 9.2 hsa-asg-14208hsa-asg-14208_st1 6.99 6.39 6.84 0.39 100 5.07 3.53 4.47 4.15 3.39 1.193.05 4.07 3.97 3.65 1.10 0 7.47E−04 −3.18 9.1 hsa-cand318hsa-cand318_st1 6.74 5.21 5.80 0.83 33 2.93 1.75 3.23 3.34 2.76 0.843.59 2.45 2.74 2.63 0.86 0 2.38E−04 −3.17 9.0 hsa-asg-4756hsa-asg-4756_st2 5.51 4.59 6.03 1.75 33 2.78 3.10 3.46 1.93 4.33 3.641.25 3.26 1.98 2.86 0.98 0 2.29E−03 −3.17 9.0 hsa-asg-13166hsa-asg-13166_st2 7.46 6.57 7.13 0.48 100 4.49 3.42 4.87 4.95 4.11 4.183.59 2.08 4.07 3.97 0.88 0 1.70E−04 −3.15 8.9 hsa-miR-194hsa-miR-194_st2 12.4 11.5 12.0 0.45 100 9.83 9.37 8.91 9.35 8.95 8.348.55 8.21 9.05 8.95 0.52 100 3.44E−06 −3.12 8.7 hsa-asg-13135hsa-asg-13135_st1 8.24 8.21 8.25 0.05 100 5.62 5.67 5.24 4.91 4.79 4.755.16 4.47 5.60 5.13 0.43 22 2.88E−07 −3.12 8.7 hsa-miR-485-3phsa-miR23_st2 6.88 6.01 6.38 0.45 67 4.31 2.54 4.12 3.28 2.45 3.26 3.592.78 3.09 3.27 0.65 0 1.86E−05 −3.11 8.6 hsa-miR-485-3phsa-miR-485-3p_st2 6.34 6.19 6.18 0.16 67 4.30 4.43 4.27 3.13 3.68 3.543.87 3.77 0.50 0 1.23E−05 −2.41 5.3 hsa-asg-4283 hsa-asg-4283_st2 5.895.01 6.31 1.56 67 3.93 3.53 2.66 3.82 3.93 2.85 3.50 2.27 2.54 3.22 0.650 4.61E−04 −3.09 8.5 hsa-asg-9980 hsa-asg-9980_st1 7.15 6.60 7.00 0.35100 5.18 4.49 4.02 1.72 3.39 2.85 3.85 5.08 4.67 3.92 1.12 11 1.06E−03−3.09 8.5 hsa-miR-130b hsa-miR-130b_st1 11.1 10.9 11.0 0.11 100 8.138.06 8.95 7.75 7.52 7.34 7.94 7.71 8.10 7.95 0.46 100 6.55E−07 −3.07 8.4hsa-asg-13878 hsa-asg-13878_st2 6.13 4.53 6.31 1.88 33 2.93 3.53 2.864.84 4.27 3.37 3.05 2.45 1.86 3.24 0.90 0 2.72E−03 −3.07 8.4hsa-asg-9499 hsa-asg-9499_st2 3.90 5.42 4.90 0.86 33 3.33 1.34 1.66 2.332.28 1.01 0.62 2.62 1.38 1.84 0.86 0 3.38E−04 −3.06 8.3 hsa-cand26hsa-cand26_st2 8.17 7.90 8.06 0.14 100 6.12 5.61 6.03 4.08 3.68 4.755.29 4.07 5.46 5.01 0.90 22 2.13E−04 −3.05 8.3 hsa-asg-9866hsa-asg-9866_st1 6.27 6.47 6.15 0.39 100 2.93 2.16 3.06 3.15 3.04 3.133.40 3.32 3.87 3.12 0.45 0 1.24E−06 −3.03 8.2 hsa-asg-3251hsa-asg-3251_st1 7.04 6.62 6.58 0.48 100 4.24 4.16 4.02 2.85 3.54 3.952.92 3.07 3.25 3.56 0.55 0 7.35E−06 −3.02 8.1 hsa-asg-11160hsa-asg-11160_st2 6.56 6.21 6.25 0.29 67 2.78 3.16 3.39 3.08 2.90 2.692.48 3.54 5.14 3.24 0.79 0 8.73E−05 −3.01 8.1 hsa-miR-127hsa-miR-127_st1 9.89 9.11 9.53 0.39 100 6.81 5.57 7.95 7.53 7.69 5.726.59 5.49 5.44 6.53 1.02 56 6.84E−04 −2.99 8.0 hsa-asg-8174hsa-asg-8174_st1 6.56 5.91 6.23 0.33 67 3.33 4.08 2.86 3.01 2.90 3.262.78 2.78 4.16 3.24 0.53 0 4.32E−06 −2.99 7.9 hsa-asg-10175hsa-asg-10175_st2 5.05 3.34 5.17 1.89 33 2.43 2.54 1.92 2.03 1.83 2.922.32 1.48 2.54 2.22 0.44 0 8.15E−04 −2.94 7.7 hsa-asg-11361hsa-asg-11361_st2 6.49 6.52 6.36 0.24 100 2.78 3.53 3.39 3.28 3.63 3.373.85 2.62 4.32 3.42 0.52 0 3.10E−06 −2.94 7.7 hsa-miR-592hsa-miR-592_st1 5.05 6.17 5.90 0.76 67 4.31 2.54 2.86 3.15 1.83 1.783.05 2.62 4.54 2.96 0.96 0 7.30E−04 −2.94 7.7 hsa-miR-592hsa-asg-4400_st1 5.13 5.86 5.61 0.42 33 3.07 3.29 3.67 1.72 1.64 2.693.05 1.68 5.42 2.92 1.21 11 4.18E−03 −2.69 6.5 hsa-asg-280hsa-asg-280_st1 6.09 6.41 5.93 0.58 67 3.21 2.71 4.47 2.13 2.53 2.171.79 3.44 4.54 3.00 1.00 0 7.93E−04 −2.93 7.6 hsa-asg-1203hsa-asg-1203_st1 6.34 5.60 6.66 1.26 67 4.49 4.37 2.66 3.82 4.21 4.553.93 3.74 1.86 3.74 0.91 0 1.25E−03 −2.93 7.6 hsa-cand206hsa-cand206_st1 5.79 5.81 5.59 0.36 67 2.25 1.96 3.79 3.63 2.69 2.173.59 1.29 2.74 2.68 0.86 0 2.33E−04 −2.91 7.5 hsa-asg-5190hsa-asg-5190_st1 6.01 5.10 5.57 0.45 67 3.76 2.54 2.18 2.69 2.76 3.371.79 2.27 2.54 2.66 0.60 0 1.86E−05 −2.91 7.5 hsa-asg-7464hsa-asg-7464_st2 6.69 5.99 6.45 0.40 67 4.61 3.92 4.81 2.51 4.00 2.173.68 2.27 3.87 3.54 0.99 0 6.77E−04 −2.91 7.5 hsa-asg-4978hsa-asg-4978_st2 4.87 5.78 5.42 0.49 33 1.65 2.87 2.86 1.72 2.01 1.972.78 2.62 4.16 2.52 0.79 0 1.47E−04 −2.91 7.5 hsa-cand330hsa-cand330_st2 7.00 5.66 7.12 1.53 67 4.76 5.04 3.54 4.71 5.15 4.182.64 3.99 3.97 4.22 0.80 11 1.36E−03 −2.90 7.5 hsa-asg-7159hsa-asg-7159_st2 5.79 5.03 6.27 1.53 33 3.56 3.29 2.43 3.95 3.93 3.484.00 3.44 2.33 3.38 0.62 0 6.10E−04 −2.89 7.4 hsa-asg-8668hsa-asg-8668_st2 6.44 5.33 6.74 1.58 67 4.55 4.16 3.23 5.06 5.07 4.553.59 2.45 2.10 3.86 1.09 0 4.96E−03 −2.88 7.4 hsa-miR-200a*hsa-miR-200a-_st2 6.34 4.91 5.58 0.72 67 3.07 2.87 2.18 3.91 2.61 1.782.15 3.20 2.92 2.74 0.65 0 7.55E−05 −2.84 7.1 hsa-miR-134hsa-miR-134_st2 7.89 7.97 8.02 0.16 100 5.11 5.78 5.99 5.85 5.70 4.214.73 4.31 5.02 5.19 0.68 0 3.85E−05 −2.83 7.1 hsa-asg-3373hsa-asg-3373_st2 7.95 4.24 5.63 2.02 33 3.56 1.96 2.66 3.77 4.08 3.691.79 1.88 1.86 2.80 0.96 0 6.85E−03 −2.83 7.1 hsa-miR-154*hsa-miR-154-_st2 6.73 7.02 7.15 0.51 100 5.83 3.53 4.21 4.99 4.63 3.954.27 3.20 4.40 4.33 0.78 11 1.78E−04 −2.82 7.1 hsa-asg-13471hsa-asg-13471_st1 7.13 6.24 7.45 1.41 100 4.76 4.76 4.39 5.12 5.35 5.404.38 4.07 3.53 4.64 0.61 11 4.95E−04 −2.82 7.0 hsa-miR-323hsa-miR-323_st1 7.12 6.57 6.72 0.35 33 4.17 3.47 4.02 5.77 5.12 2.993.64 2.93 3.25 3.93 0.97 0 7.71E−04 −2.79 6.9 hsa-asg-13922hsa-asg-13922_st1 6.21 6.53 6.66 0.53 100 4.71 5.40 1.66 4.84 5.58 5.174.07 2.27 1.38 3.90 1.67 0 2.08E−02 −2.76 6.8 hsa-miR-485-5phsa-miR-485-5p_st2 5.74 6.05 5.85 0.17 33 3.45 3.29 3.39 3.68 3.28 1.973.50 1.88 3.53 3.11 0.68 0 5.24E−05 −2.74 6.7 hsa-miR-382hsa-miR-382_st1 9.24 9.51 9.46 0.20 100 7.17 6.85 7.35 7.13 7.43 6.566.29 5.93 5.81 6.72 0.61 78 2.20E−05 −2.73 6.7 hsa-asg-13928hsa-asg-13928_st1 5.63 4.96 6.25 1.69 33 4.55 3.69 2.66 3.40 3.97 4.182.99 1.88 4.54 3.54 0.90 0 4.19E−03 −2.71 6.6 hsa-asg-1596hsa-asg-1596_st2 7.15 6.69 6.94 0.23 100 4.43 5.12 4.02 3.21 4.39 4.184.14 4.21 4.40 4.23 0.50 0 4.57E−06 −2.70 6.5 hsa-asg-10881hsa-asg-10881_st1 7.92 7.62 7.90 0.27 100 5.73 5.04 6.06 5.43 4.79 5.464.20 5.04 5.23 5.22 0.54 22 1.12E−05 −2.68 6.4 hsa-miR-409-3phsa-miR-409-3p_st1 7.36 6.04 6.45 0.79 33 2.25 3.47 5.14 4.99 4.77 2.993.96 2.62 3.77 3.77 1.05 0 2.48E−03 −2.67 6.4 hsa-miR-203hsa-miR-203_st2 10.8 8.88 9.45 1.18 100 7.33 5.89 7.21 8.24 6.45 5.415.19 6.07 9.36 6.79 1.37 78 1.37E−02 −2.66 6.3 hsa-asg-2844hsa-asg-2844_st1 4.49 4.27 5.52 1.97 33 3.76 4.23 2.18 2.85 2.01 2.992.78 2.62 2.54 2.89 0.71 0 4.58E−03 −2.64 6.2 hsa-miR-379hsa-miR-379_st1 9.33 9.04 9.22 0.16 100 6.52 6.42 7.11 6.97 7.04 6.596.35 6.14 6.23 6.60 0.36 100 3.15E−07 −2.63 6.2 hsa-miR-148ahsa-miR-148a_st1 12.9 13.1 13.2 0.30 100 12.1 11.1 11.5 10.5 10.2 11.010.2 9.18 9.38 10.6 0.96 100 1.16E−03 −2.61 6.1 hsa-asg-12907hsa-asg-12907_st2 5.51 4.91 5.32 0.36 33 3.76 2.87 3.54 2.51 2.83 1.972.78 1.68 2.54 2.72 0.66 0 8.14E−05 −2.60 6.0 hsa-asg-10309hsa-asg-10309_st1 6.05 7.02 6.81 0.68 100 5.25 5.79 2.18 4.66 6.03 5.013.93 3.07 2.33 4.25 1.45 33 1.63E−02 −2.56 5.9 hsa-asg-9510hsa-asg-9510_st1 8.23 8.74 8.78 0.57 67 6.93 7.30 4.98 6.81 8.07 7.156.29 4.66 3.87 6.23 1.41 0 1.37E−02 −2.55 5.9 hsa-miR-369-5phsa-miR-369-5p_st2 6.72 5.35 6.00 0.69 67 3.56 2.71 4.47 4.36 4.36 2.532.92 2.62 3.53 3.45 0.80 0 5.92E−04 −2.55 5.9 hsa-miR-369-5phsa-asg-1521_st1 6.35 5.06 5.75 0.65 33 3.76 3.42 4.02 2.23 3.72 3.733.40 2.78 3.53 3.40 0.56 0 1.16E−04 −2.35 5.1 hsa-asg-4472hsa-asg-4472_st2 5.91 3.60 5.78 2.12 33 3.76 4.00 2.86 2.93 3.63 4.342.92 1.88 2.74 3.23 0.76 0 8.37E−03 −2.55 5.9 hsa-asg-4542hsa-asg-4542_st2 7.40 6.75 7.50 0.81 100 5.94 4.99 4.12 6.59 6.41 5.633.77 3.07 4.32 4.98 1.24 33 8.82E−03 −2.52 5.7 hsa-asg-12515hsa-asg-12515_st2 6.15 5.17 6.51 1.55 33 4.02 4.30 3.06 4.12 4.60 4.373.68 4.14 3.77 4.01 0.46 0 8.87E−04 −2.50 5.7 hsa-asg-2845hsa-asg-2845_st2 5.63 3.47 5.50 1.96 33 2.93 3.83 3.06 3.68 2.01 3.193.05 2.78 2.54 3.01 0.55 0 4.03E−03 −2.49 5.6 hsa-miR-299-5phsa-miR-299-5p_st2 7.53 6.63 7.21 0.50 100 5.73 4.49 5.80 6.38 5.99 2.995.05 2.45 3.97 4.76 1.39 44 1.57E−02 −2.44 5.4 hsa-asg-1268hsa-asg-1268_st1 6.39 5.72 6.12 0.36 67 4.76 3.29 4.02 4.15 3.49 3.783.85 2.27 3.65 3.70 0.68 0 1.80E−04 −2.43 5.4 hsa-asg-548hsa-asg-548_st1 5.89 5.42 5.50 0.36 33 3.07 1.96 3.54 3.52 2.83 1.973.40 2.78 4.60 3.07 0.83 0 7.14E−04 −2.42 5.4 hsa-asg-3807hsa-asg-3807_st1 6.40 5.42 5.64 0.68 33 3.07 3.42 3.06 3.95 3.89 1.683.40 2.93 3.65 3.23 0.68 0 3.52E−04 −2.41 5.3 hsa-asg-6283hsa-asg-6283_st1 6.30 6.49 6.61 0.37 100 3.85 5.48 3.31 4.60 6.35 5.224.98 2.17 1.98 4.22 1.50 11 2.43E−02 −2.39 5.2 hsa-asg-12666hsa-asg-12666_st2 7.63 6.75 7.21 0.44 100 6.08 4.66 4.87 4.91 4.65 4.604.82 3.99 4.84 4.83 0.55 11 4.79E−05 −2.39 5.2 hsa-asg-4840hsa-asg-4840_st1 6.70 6.45 7.21 1.11 100 5.45 5.08 3.54 5.28 5.43 5.034.64 4.58 4.40 4.83 0.61 11 6.83E−04 −2.39 5.2 hsa-asg-6619hsa-asg-6619_st1 4.92 4.06 5.15 1.22 33 2.61 0.54 2.05 4.71 2.90 4.283.50 2.36 1.98 2.77 1.27 0 1.78E−02 −2.38 5.2 hsa-asg-10236hsa-asg-10236_st2 6.01 4.53 6.18 1.75 33 4.09 3.53 3.06 4.52 5.17 3.783.17 3.74 3.39 3.83 0.68 0 5.11E−03 −2.36 5.1 hsa-miR-494hsa-miR-494_st1 9.21 8.79 9.06 0.24 100 6.40 7.30 7.22 6.96 6.63 7.665.73 5.86 6.60 6.71 0.65 100 1.35E−04 −2.35 5.1 hsa-miR-494hsa-miR61_st2 8.60 7.71 8.20 0.45 100 6.83 6.55 6.85 6.43 6.38 7.10 5.405.79 6.89 6.47 0.55 89 6.64E−04 −1.74 3.3 hsa-asg-3185 hsa-asg-3185_st15.91 4.69 6.14 1.58 33 4.49 4.27 2.76 4.93 4.53 5.27 2.32 2.53 3.17 3.811.11 0 1.68E−02 −2.33 5.0 hsa-asg-7038 hsa-asg-7038_st1 3.90 1.79 4.132.46 33 1.45 0.54 2.76 3.08 2.37 0.68 2.15 1.98 1.26 1.81 0.89 02.79E−02 −2.32 5.0 hsa-asg-67 hsa-asg-67_st2 6.61 5.19 5.86 0.71 67 4.553.29 4.02 2.33 3.22 3.59 3.68 3.54 3.65 3.54 0.60 0 2.42E−04 −2.32 5.0hsa-miR-129 hsa-miR-129_st1 8.28 6.08 6.85 1.24 67 4.85 4.81 5.24 4.814.21 3.95 4.49 4.58 4.40 4.59 0.39 0 4.13E−04 −2.26 4.8 hsa-asg-3873hsa-asg-3873_st1 5.17 4.24 5.33 1.18 33 4.17 2.26 2.54 1.72 3.77 3.823.40 3.38 2.64 3.08 0.82 0 3.91E−03 −2.25 4.8 hsa-miR-376bhsa-miR-376b_st1 4.96 3.47 4.55 0.94 33 1.85 2.16 4.12 3.34 2.69 1.012.78 2.53 0.21 2.30 1.18 0 1.39E−02 −2.25 4.8 hsa-asg-3081hsa-asg-3081_st1 5.66 6.11 6.23 0.63 33 4.76 4.49 3.23 4.30 5.75 4.553.77 3.91 1.38 4.01 1.21 11 1.44E−02 −2.21 4.6 hsa-miR-329hsa-asg-13589_st2 6.32 4.49 5.39 0.91 33 1.65 3.16 3.79 3.73 4.42 2.353.68 2.78 3.09 3.18 0.84 0 3.11E−03 −2.21 4.6 hsa-miR-329hsa-miR-329_st1 5.66 5.27 5.47 0.19 33 3.76 3.02 4.61 3.28 4.58 2.852.78 3.32 2.74 3.44 0.73 0 9.45E−04 −2.03 4.1 hsa-miR-329hsa-asg-13204_st2 5.79 3.92 5.08 1.01 67 4.09 2.87 4.21 3.40 4.24 3.484.00 2.62 3.53 3.61 0.59 0 9.50E−03 −1.48 2.8 hsa-asg-9512hsa-asg-9512_st1 8.12 8.25 8.33 0.27 100 6.42 6.94 4.61 6.58 7.81 7.056.37 5.29 5.23 6.25 1.02 11 6.92E−03 −2.08 4.2 hsa-asg-5996hsa-asg-5996_st2 7.97 8.69 8.72 0.77 67 8.10 7.67 5.28 7.68 8.03 7.006.23 6.39 3.77 6.68 1.44 0 4.47E−02 −2.04 4.1 hsa-asg-2299hsa-asg-2299_st1 5.45 5.46 5.66 0.36 67 4.71 4.16 4.54 3.63 3.44 3.593.40 2.27 2.92 3.63 0.77 0 1.50E−03 −2.04 4.1 hsa-asg-8266hsa-asg-8266_st2 6.21 6.62 6.35 0.23 100 4.17 3.83 4.61 5.23 4.45 4.754.49 3.44 3.87 4.32 0.55 0 1.15E−04 −2.03 4.1 hsa-asg-9553hsa-asg-9553_st1 4.22 2.95 4.64 1.94 33 2.78 3.23 0.79 4.15 3.39 3.322.92 1.02 2.44 2.67 1.11 0 4.85E−02 −1.97 3.9 hsa-asg-14048hsa-asg-14048_st1 6.27 6.26 6.26 0.01 67 4.43 4.95 3.54 3.63 5.03 3.264.59 5.15 4.07 4.29 0.70 0 8.80E−04 −1.96 3.9 hsa-asg-10775hsa-asg-10775_st2 7.03 6.54 6.71 0.28 100 5.11 4.76 4.81 5.06 4.36 4.684.82 4.52 4.73 4.76 0.23 0 2.95E−07 −1.95 3.9 hsa-miR-365hsa-cand708_st1 7.75 6.59 6.75 0.93 100 5.94 5.23 4.02 6.58 5.46 4.245.64 3.07 3.53 4.86 1.19 44 3.19E−02 −1.90 3.7 hsa-miR-365hsa-miR-365_st1 8.62 7.33 7.69 0.82 100 7.27 5.64 5.53 7.63 6.38 5.406.66 4.78 4.78 6.01 1.03 56 2.98E−02 −1.68 3.2 hsa-miR-365hsa-asg-13156_st1 8.91 7.47 7.69 1.14 100 7.33 5.87 6.09 7.37 6.38 5.466.63 4.78 4.67 6.06 0.98 56 3.73E−02 −1.62 3.1 hsa-asg-7751hsa-asg-7751_st2 5.39 4.77 5.33 0.54 33 2.93 3.74 3.39 2.77 5.07 5.134.07 1.68 2.33 3.46 1.17 0 2.57E−02 −1.88 3.7 hsa-asg-4069hsa-asg-4069_st1 6.05 4.19 5.98 1.76 33 4.31 4.93 2.76 4.46 4.30 4.974.14 3.59 3.92 4.15 0.68 0 2.02E−02 −1.83 3.6 hsa-asg-1451hsa-asg-1451_st1 5.32 6.06 5.32 0.74 33 3.66 2.71 4.12 2.69 3.44 3.872.92 4.47 3.87 3.53 0.63 0 2.13E−03 −1.80 3.5 hsa-asg-13077hsa-asg-13077_st1 5.91 6.47 6.57 0.72 100 5.98 5.12 3.23 5.27 6.03 5.054.54 3.91 3.97 4.79 0.95 44 1.49E−02 −1.79 3.4 hsa-asg-13800hsa-asg-13800_st2 4.36 4.49 4.91 0.83 33 4.24 3.64 3.79 3.15 3.10 3.481.25 2.78 2.74 3.13 0.86 0 1.06E−02 −1.78 3.4 hsa-asg-7540hsa-asg-7540_st2 5.98 5.56 5.81 0.22 67 3.76 4.43 3.06 3.52 4.14 4.973.93 4.47 3.97 4.03 0.56 0 4.11E−04 −1.78 3.4 hsa-asg-1301hsa-asg-1301_st1 5.63 5.96 5.56 0.44 100 4.49 3.74 3.91 3.73 3.81 3.694.20 3.83 2.92 3.81 0.42 0 1.10E−04 −1.75 3.4 hsa-miR-189hsa-miR-189_st1 7.57 6.64 7.07 0.47 100 6.08 5.79 4.93 7.23 6.18 4.554.94 3.54 4.95 5.36 1.08 44 2.71E−02 −1.71 3.3 hsa-asg-10830hsa-asg-10830_st2 6.17 5.35 5.62 0.48 67 4.37 4.00 3.67 3.91 3.93 4.493.40 3.64 3.87 3.92 0.35 0 4.94E−05 −1.70 3.2 hsa-miR-30a-3phsa-miR-30a-3p_st1 10.6 10.0 10.2 0.33 100 9.33 9.03 8.26 9.31 8.40 9.137.93 7.39 8.19 8.55 0.68 100 2.35E−03 −1.69 3.2 hsa-miR-29chsa-miR-29c_st2 11.2 10.4 10.9 0.42 100 10.0 9.44 9.16 9.78 9.72 7.508.94 8.77 9.53 9.21 0.76 100 4.92E−03 −1.69 3.2 hsa-asg-12356hsa-asg-12356_st1 8.24 9.20 9.06 0.76 100 7.95 8.62 5.85 7.75 8.67 8.306.83 7.02 6.03 7.45 1.06 78 3.73E−02 −1.62 3.1 hsa-asg-677hsa-asg-677_st2 5.17 5.39 5.68 0.70 33 3.45 4.71 3.91 4.15 5.07 4.803.68 4.14 2.74 4.07 0.73 0 7.75E−03 −1.60 3.0 hsa-asg-3009hsa-asg-3009_st1 5.77 6.04 6.37 0.82 33 5.18 5.62 4.34 5.21 5.43 5.053.50 4.44 4.20 4.78 0.69 0 7.80E−03 −1.60 3.0 hsa-asg-3376hsa-asg-3376_st1 5.58 5.06 5.44 0.34 33 3.85 3.02 4.87 4.66 4.00 4.182.48 4.21 3.39 3.85 0.77 0 6.99E−03 −1.58 3.0 hsa-asg-3597hsa-asg-3597_st2 7.70 8.11 8.18 0.52 100 6.64 7.77 5.06 6.88 7.77 6.986.69 5.98 5.80 6.62 0.89 78 1.80E−02 −1.57 3.0 hsa-asg-381hsa-asg-381_st1 5.05 3.53 4.88 1.27 33 3.07 3.64 3.06 3.68 3.85 3.373.50 3.32 2.54 3.34 0.40 0 6.20E−03 −1.54 2.9 hsa-miR-204hsa-miR-204_st2 8.51 7.05 7.70 0.74 100 7.18 5.82 5.98 8.21 6.76 5.465.19 5.42 5.78 6.20 0.99 44 3.85E−02 −1.50 2.8 hsa-asg-12990hsa-asg-12990_st1 2.96 2.47 3.65 1.64 33 2.61 2.71 2.43 1.93 2.37 2.172.48 2.27 0.73 2.19 0.59 0 3.58E−02 −1.47 2.8 hsa-asg-7471hsa-asg-7471_st1 7.01 7.01 7.12 0.19 100 5.60 5.72 5.67 6.74 5.70 5.305.25 5.00 6.09 5.68 0.51 56 9.23E−04 −1.44 2.7 hsa-asg-8476hsa-asg-8476_st1 7.42 7.51 7.48 0.05 33 5.60 6.42 6.43 6.33 5.78 6.226.76 5.42 5.63 6.06 0.47 11 4.80E−04 −1.42 2.7 hsa-miR-409-5phsa-miR-409-5p_st1 7.28 6.87 7.06 0.21 100 5.14 5.04 6.67 6.09 6.11 5.175.92 5.45 5.27 5.65 0.57 44 2.16E−03 −1.41 2.7 hsa-miR-620hsa-miR-620_st1 4.15 4.19 4.20 0.05 67 3.56 3.02 1.92 2.51 3.22 3.371.25 3.20 3.09 2.79 0.76 0 1.14E−02 −1.40 2.6 hsa-asg-3742hsa-asg-3742_st1 5.05 5.25 5.44 0.51 33 3.85 4.95 3.67 4.26 4.87 3.483.59 4.21 3.65 4.06 0.55 0 3.47E−03 −1.38 2.6 hsa-cand533hsa-cand533_st1 5.32 5.66 5.46 0.18 33 4.85 4.23 4.12 4.49 3.89 3.593.50 4.07 4.24 4.11 0.42 0 3.75E−04 −1.35 2.5 hsa-asg-12208hsa-asg-12208_st1 5.72 5.42 6.07 0.87 33 4.24 3.83 5.24 4.99 5.07 5.174.94 4.83 4.24 4.73 0.50 0 6.78E−03 −1.34 2.5 hsa-asg-4163hsa-asg-4163_st2 8.04 6.81 7.53 0.64 100 6.23 6.02 6.45 6.44 6.90 5.875.92 5.90 6.09 6.20 0.34 100 7.75E−04 −1.33 2.5 hsa-asg-3798hsa-asg-3798_st1 7.52 6.57 6.73 0.72 100 5.68 4.81 5.71 5.77 5.54 5.015.12 5.29 5.78 5.41 0.36 22 1.54E−03 −1.32 2.5 hsa-asg-414hsa-asg-414_st1 7.32 6.95 7.59 0.82 100 6.29 6.32 6.65 6.16 6.04 6.876.62 5.74 5.92 6.29 0.37 78 2.64E−03 −1.30 2.5 hsa-asg-3672hsa-asg-3672_st1 5.42 6.51 6.51 1.08 67 5.35 6.54 4.17 5.37 5.75 6.054.59 4.49 4.50 5.20 0.81 11 4.91E−02 −1.30 2.5 hsa-miR-30e-5phsa-miR-30e-5p_st2 11.6 10.3 10.8 0.69 100 10.4 9.64 9.71 10.5 9.81 9.239.23 8.15 9.50 9.58 0.70 100 1.94E−02 −1.30 2.5 hsa-cand283hsa-cand283_st1 6.95 5.91 6.47 0.52 67 5.45 6.14 4.68 5.56 4.74 5.635.25 4.58 4.54 5.17 0.57 33 5.90E−03 −1.29 2.5 hsa-miR-30chsa-miR-30c_st1 12.5 12.2 12.4 0.20 100 11.6 11.6 11.2 11.9 11.5 11.611.1 10.1 10.0 11.2 0.68 100 1.14E−02 −1.26 2.4 hsa-miR-95hsa-miR-95_st1 11.0 10.0 10.2 0.66 100 9.39 8.65 9.82 10.1 9.57 8.449.10 7.82 8.21 9.02 0.79 100 3.33E−02 −1.26 2.4 hsa-asg-12885hsa-asg-12885_st1 6.65 6.36 6.50 0.15 100 6.66 5.30 5.41 5.53 5.41 5.435.25 3.44 5.31 5.30 0.82 89 3.51E−02 −1.20 2.3 hsa-miR-27bhsa-miR-27b_st1 13.7 13.3 13.4 0.21 100 12.5 12.3 12.0 13.2 12.8 11.911.9 11.0 12.5 12.2 0.64 100 1.11E−02 −1.20 2.3 hsa-cand443hsa-cand443_st2 5.60 5.31 5.77 0.57 67 3.93 5.15 3.91 4.49 5.76 5.274.86 3.83 4.32 4.61 0.69 33 2.57E−02 −1.16 2.2 hsa-asg-10626hsa-asg-10626_st2 6.15 6.45 6.00 0.54 100 5.14 5.19 4.61 5.37 4.99 4.314.86 4.28 5.00 4.86 0.39 0 2.31E−03 −1.14 2.2 hsa-miR-30bhsa-miR-30b_st2 12.4 11.6 12.1 0.43 100 11.6 11.2 11.1 11.5 11.1 10.910.9 10.0 9.96 10.9 0.58 100 1.17E−02 −1.14 2.2 hsa-asg-1380hsa-asg-1380_st2 4.55 4.69 4.73 0.21 33 4.09 3.42 1.66 4.08 4.04 3.953.40 4.41 3.39 3.60 0.82 0 4.42E−02 −1.13 2.2 hsa-asg-259hsa-asg-259_st2 5.63 5.31 5.41 0.19 33 5.29 4.76 4.54 5.04 4.42 4.183.29 3.91 3.25 4.30 0.72 22 2.70E−02 −1.11 2.2 hsa-asg-3300hsa-asg-3300_st2 6.03 7.28 6.16 1.06 67 4.31 4.30 4.87 6.05 4.97 5.055.61 5.22 5.14 5.06 0.56 22 3.71E−02 −1.10 2.1 hsa-asg-2967hsa-asg-2967_st2 6.15 5.81 5.81 0.34 67 5.18 4.86 4.54 4.81 5.95 4.433.93 4.35 4.32 4.71 0.59 22 1.34E−02 −1.10 2.1 hsa-asg-9009hsa-asg-9009_st1 5.13 5.58 5.72 0.67 33 5.03 5.57 4.17 4.49 4.45 5.254.94 3.69 4.02 4.62 0.62 0 2.55E−02 −1.10 2.1 hsa-asg-12874hsa-asg-12874_st1 5.35 4.19 5.53 1.43 33 4.02 4.23 4.02 4.86 4.60 4.894.20 4.52 4.67 4.45 0.34 0 4.46E−02 −1.08 2.1 hsa-asg-2315hsa-asg-2315_st2 5.45 5.61 5.89 0.62 33 5.60 5.83 4.58 4.66 5.39 4.913.68 4.85 3.92 4.82 0.72 0 4.62E−02 −1.06 2.1 hsa-miR-148bhsa-miR-148b_st2 10.7 9.42 10.2 0.71 100 10.0 8.79 9.44 10.0 9.63 8.279.09 8.14 9.14 9.18 0.70 100 4.68E−02 −1.06 2.1 hsa-asg-4448hsa-asg-4448_st1 7.21 6.87 7.00 0.19 100 6.73 6.14 5.98 7.05 6.17 5.016.26 4.87 5.31 5.95 0.75 67 4.13E−02 −1.05 2.1 hsa-asg-6616hsa-asg-6616_st1 6.74 6.48 7.00 0.68 100 5.42 5.69 6.55 6.54 5.50 6.005.29 6.05 6.75 5.98 0.54 78 2.28E−02 −1.03 2.0 hsa-asg-12807hsa-asg-12807_st2 6.50 7.28 6.97 0.41 100 5.89 5.96 6.99 6.57 5.61 5.055.05 6.49 6.00 5.96 0.66 78 3.38E−02 −1.01 2.0 SD, standard deviation;%, percentage of samples expressing miRNA above background levels;Log2Diff, difference in VSN-transformed expression bewteen PAN and LNnegsamples.

Example 27 miRNAs Differentally Expressed Between Normal Thyroid TissueAnd Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous thyroid cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal thyroidtissue than in cancer-negative lymph nodes.

Total RNA, purified from one normal human thyroid sample (FirstChoice®Human Thyroid Total RNA, cat. no. AM6872; Ambion, Austin, Tex., USA) andfrom nine normal human lymph node samples (FirstChoice® Human Lymph NodeTotal RNA, cat. no. AM7894; Ambion, Austin, Tex., USA) was purchasedfrom Ambion. miRNA purification and labeling and microarray expressionanalyses were performed as described above in Example 2.

The inventors identified 362 miRNAs (31 verified, 331 predicted) withsignificant differential expression between the normal thyroid sampleand the normal lymph nodes (|log 2Diff| thyroid vs LN neg≧1 andp-value<0.05) (Table 26). Among the verified miRNAs, hsa-miR-206 wasexpressed more than 300-fold higher in the thyroid tissue; hsa-miR-133b,-499, and -133a were expressed between 50- and 100-fold higher in thethyroid sample; eleven miRNAs were expressed between 10- and 50-foldhigher in the thyroid sample; and seven miRNAs were expressed between 5-and 10-fold higher in the thyroid tissue than in the normal lymph nodes.Among the predicted miRNAs, hsa-asg-4915-st1 and -2027-st1 wereexpressed between 25- and 40-fold higher in the thyroid tissue sample;19 miRNAs were expressed between 10- and 20-fold higher in the thyroidsample; and 112 miRNAs were expressed between 5- and 10-fold higher inthe thyroid sample than in normal lymph nodes.

TABLE 26 MicroRNAs Differentially Expressed Between Normal Thyroid (THY)and Cancer-Negative Lymph Node Samples (LNneg) from Patients. LNnegMicro RNA Probe ID THY 1 2 3 4 5 6 7 8 9 hsa-miR-206 hsa-miR-206_st213.83 4.07 4.94 1.89 9.39 3.93 5.79 8.45 4.44 4.97 hsa-miR-133bhsa-miR-133b_st2 12.92 7.35 6.17 4.46 10.1 7.42 6.15 7.20 4.44 5.36hsa-miR-499 hsa-miR12_st1 11.17 5.44 4.76 5.37 6.66 4.07 4.38 4.80 4.553.84 hsa-miR-499 hsa-miR-499_st1 10.99 5.31 4.61 4.75 6.57 4.49 4.125.03 4.25 4.58 hsa-miR-133a hsa-miR-133a_st1 13.35 7.76 7.65 5.93 10.88.16 7.08 8.20 6.23 6.46 hsa-asg-4915_st1 hsa-asg-4915_st1 8.12 2.413.78 2.15 2.57 3.59 2.87 2.77 2.05 4.44 hsa-miR-429 hsa-miR-429_st1 8.003.42 1.54 3.66 2.10 2.33 2.65 2.31 2.41 5.36 hsa-miR-200bhsa-miR-200b_st1 12.55 7.61 7.75 8.91 6.69 6.79 6.82 6.50 7.64 10.4hsa-asg-2027_st1 hsa-asg-2027_st1 8.72 3.53 3.48 1.63 5.22 4.44 4.194.25 4.19 4.51 hsa-miR-200a hsa-miR-200a_st2 9.99 6.26 5.02 6.49 5.715.25 4.54 4.05 4.75 7.76 hsa-miR-200c hsa-miR-200c_st1 13.25 9.59 8.7910.31 8.17 8.12 8.08 7.96 8.87 11.5 hsa-asg-2399_st2 hsa-asg-2399_st26.52 3.05 3.36 2.84 2.98 2.16 2.49 1.09 2.23 2.51 hsa-miR-605hsa-miR-605_st1 6.48 2.58 2.13 3.04 0.73 1.71 2.14 2.90 3.03 4.44hsa-asg-14153_st2 hsa-asg-14153_st2 6.62 3.05 2.23 3.04 2.98 2.33 2.651.17 2.74 4.37 hsa-asg-11715_st2 hsa-asg-11715_st2 5.66 1.83 2.32 2.151.48 2.07 1.76 0.63 1.46 3.36 hsa-asg-2484_st2 hsa-asg-2484_st2 6.282.41 2.32 1.37 3.98 2.16 2.32 3.38 2.74 2.51 hsa-asg-3983_st1hsa-asg-3983_st1 9.47 4.50 6.67 5.37 5.39 6.02 6.97 4.98 5.76 6.31hsa-asg-6283_st1 hsa-asg-6283_st1 7.88 3.83 5.43 3.30 4.59 6.32 5.164.96 2.14 1.95 hsa-miR-368 hsa-miR-368_st2 9.90 6.48 6.47 7.70 8.08 7.685.18 5.62 3.97 5.07 hsa-asg-4209_st2 hsa-asg-4209_st2 7.87 4.22 4.963.59 4.28 5.42 5.13 2.90 3.10 4.41 hsa-miR-381 hsa-miR-381_st2 6.73 2.752.32 4.21 3.38 4.17 2.94 3.48 1.28 3.22 hsa-miR-203 hsa-miR-203_st210.40 7.32 5.84 7.22 8.24 6.42 5.36 5.17 6.05 9.34 hsa-asg-10309_st1hsa-asg-10309_st1 7.81 5.24 5.74 2.15 4.65 6.00 4.96 3.91 3.03 2.29hsa-asg-2345_st2 hsa-asg-2345_st2 5.94 1.63 2.50 2.40 3.60 1.44 2.493.15 2.58 1.36 hsa-asg-10393_st1 hsa-asg-10393_st1 6.25 2.75 1.54 2.403.89 2.79 2.80 1.78 3.51 2.51 hsa-cand14_st1 hsa-cand14_st1 5.75 1.831.54 1.37 2.10 2.79 1.95 2.31 2.41 3.50 hsa-asg-1375_st1hsa-asg-1375_st1 9.62 6.84 7.16 5.37 6.52 6.89 7.05 5.17 4.93 5.07hsa-asg-12172_st2 hsa-asg-12172_st2 10.04 7.63 7.50 5.12 7.19 7.33 7.645.90 5.85 4.67 hsa-asg-6178_st2 hsa-asg-6178_st2 5.75 2.41 3.36 2.403.80 2.16 1.21 0.78 2.23 2.29 hsa-miR-539 hsa-miR177_st1 5.61 2.58 2.501.37 2.39 1.80 1.86 3.48 1.66 2.07 hsa-miR-539 hsa-miR-539_st1 5.71 2.033.11 2.63 3.38 2.65 2.94 1.96 2.58 1.36 hsa-asg-3473_st1hsa-asg-3473_st1 9.26 6.78 7.18 3.90 5.98 6.79 6.97 5.09 5.11 4.82hsa-asg-6623_st2 hsa-asg-6623_st2 9.38 6.75 7.18 5.17 6.10 6.99 6.505.00 5.55 4.67 hsa-asg-3081_st1 hsa-asg-3081_st1 7.36 4.74 4.44 3.214.28 5.72 4.49 3.75 3.88 1.36 hsa-miR-141 hsa-miR-141_st1 7.49 5.05 3.784.93 4.41 4.36 2.80 3.83 3.80 4.51 hsa-asg-12142_st1 hsa-asg-12142_st15.61 2.58 2.67 2.15 3.50 1.80 1.76 2.77 1.09 2.29 hsa-asg-1020_st2hsa-asg-1020_st2 9.21 6.39 7.36 3.45 6.17 7.05 6.87 5.90 5.18 4.90hsa-asg-3743_st1 hsa-asg-3743_st1 6.42 3.18 4.28 3.30 3.25 3.45 2.872.14 2.32 3.89 hsa-asg-1826_st2 hsa-asg-1826_st2 5.94 3.31 2.97 2.153.55 2.49 2.14 2.90 3.10 1.83 hsa-asg-4116_st2 hsa-asg-4116_st2 9.246.75 7.35 4.96 6.31 6.91 6.72 5.49 4.87 4.79 hsa-asg-924_st1hsa-asg-924_st1 6.58 4.64 4.25 3.38 4.44 2.65 2.73 2.14 2.74 3.50hsa-asg-555_st1 hsa-asg-555_st1 9.79 7.33 7.85 5.31 6.60 7.12 7.27 5.336.32 6.24 hsa-asg-9920_st1 hsa-asg-9920_st1 10.45 8.07 8.33 6.09 7.778.07 7.77 6.61 6.76 6.38 hsa-asg-20_st1 hsa-asg-20_st1 5.89 3.91 3.911.00 3.50 3.81 3.48 1.42 2.32 1.48 hsa-asg-1296_st1 hsa-asg-1296_st16.36 3.05 2.50 4.81 2.39 2.49 2.73 3.15 3.88 4.13 hsa-asg-10250_st1hsa-asg-10250_st1 5.16 1.83 2.13 1.12 2.10 2.79 1.76 3.48 2.05 1.14hsa-asg-4037_st1 hsa-asg-4037_st1 5.62 2.75 2.32 1.89 3.50 3.35 2.322.31 1.85 2.29 hsa-asg-8242_st2 hsa-asg-8242_st2 6.22 2.75 3.53 1.374.35 4.20 4.43 3.43 1.66 2.29 hsa-asg-1699_st2 hsa-asg-1699_st2 10.358.48 8.85 6.17 7.49 7.64 8.30 5.85 6.03 6.42 hsa-asg-7637_st2hsa-asg-7637_st2 6.84 3.64 2.50 4.46 3.44 3.89 3.97 3.27 3.61 5.24hsa-asg-1765_st1 hsa-asg-1765_st1 5.59 2.22 2.13 2.84 3.60 2.33 2.323.27 2.23 2.07 hsa-asg-9512_st1 hsa-asg-9512_st1 9.25 6.41 6.89 4.616.57 7.78 7.00 6.35 5.27 5.20 hsa-asg-12139_st2 hsa-asg-12139_st2 5.342.75 2.32 2.63 2.30 1.62 2.14 1.60 2.05 3.50 hsa-asg-7751_st2hsa-asg-7751_st2 6.42 2.90 3.68 3.38 2.74 5.03 5.07 4.05 1.66 2.29hsa-asg-7292_st1 hsa-asg-7292_st1 10.73 8.54 8.83 6.92 7.90 7.96 8.426.87 7.27 6.96 hsa-asg-128_st2 hsa-asg-128_st2 7.56 4.84 4.71 4.54 5.114.52 4.12 4.96 4.32 4.13 hsa-asg-7550_st1 hsa-asg-7550_st1 6.38 3.424.52 2.28 4.82 3.06 3.53 2.90 2.66 3.43 hsa-miR-376a hsa-miR-376a_st27.30 5.13 4.03 5.49 5.66 4.82 3.97 2.47 3.80 3.73 hsa-miR-376ahsa-asg-10049_st1 7.69 5.01 4.85 6.09 5.55 5.31 4.05 3.83 4.12 4.64hsa-miR-376a hsa-asg-14234_st1 6.30 3.31 4.25 3.38 3.98 4.82 3.63 2.904.04 1.83 hsa-asg-10131_st2 hsa-asg-10131_st2 6.13 3.31 3.11 3.52 2.482.49 3.20 2.62 3.17 5.07 hsa-asg-13070_st1 hsa-asg-13070_st1 5.44 2.902.50 2.63 4.14 3.06 2.14 2.31 1.85 1.36 hsa-miR-95 hsa-miR-95_st1 11.899.38 8.60 9.83 10.1 9.54 8.39 9.08 7.80 8.19 hsa-asg-4864_st1hsa-asg-4864_st1 9.22 6.95 7.68 5.26 6.60 6.98 7.46 5.82 6.31 4.08hsa-asg-3597_st2 hsa-asg-3597_st2 9.46 6.62 7.72 5.07 6.87 7.74 6.936.67 5.96 5.77 hsa-asg-9201_st2 hsa-asg-9201_st2 8.33 6.88 6.16 4.255.61 5.85 6.11 4.57 5.18 4.67 hsa-asg-8079_st1 hsa-asg-8079_st1 7.575.05 6.14 3.72 4.17 5.51 5.11 3.66 3.75 5.30 hsa-miR-135bhsa-miR-135b_st2 5.41 1.43 2.67 2.40 2.82 2.33 3.08 3.27 2.23 2.89hsa-miR-135b hsa-cand78_st1 5.34 3.42 2.50 3.66 4.14 3.68 2.80 3.57 1.663.22 hsa-asg-13888_st2 hsa-asg-13888_st2 5.07 1.43 2.83 1.63 3.12 1.802.14 2.62 1.85 2.71 hsa-asg-11777_st1 hsa-asg-11777_st1 5.32 2.58 2.131.89 3.60 1.98 1.95 2.90 2.23 3.06 hsa-miR-138 hsa-miR-138_st1 8.41 6.365.68 6.82 6.41 5.91 4.57 4.92 4.87 5.09 hsa-asg-10080_st1hsa-asg-10080_st1 5.07 2.03 1.74 2.40 1.79 2.33 2.40 2.47 2.23 3.36hsa-asg-5119_st1 hsa-asg-5119_st1 5.27 2.41 2.67 1.89 1.90 1.98 2.492.90 2.58 3.62 hsa-asg-9082_st1 hsa-asg-9082_st1 5.75 2.75 4.47 1.242.90 3.06 3.63 2.47 3.56 2.80 hsa-asg-5728_st2 hsa-asg-5728_st2 9.116.01 7.42 5.43 6.29 6.37 7.04 5.60 6.37 6.65 hsa-asg-5189_st2hsa-asg-5189_st2 10.60 8.62 9.20 6.50 8.16 8.47 8.78 7.08 7.31 6.63hsa-asg-677_st2 hsa-asg-677_st2 6.78 3.42 4.66 3.90 4.14 5.03 4.74 3.664.12 2.71 hsa-asg-13077_st1 hsa-asg-13077_st1 7.49 5.97 5.06 3.21 5.266.00 5.00 4.52 3.88 3.94 hsa-asg-3822_st2 hsa-asg-3822_st2 5.44 2.032.97 3.66 2.00 2.41 2.14 1.60 2.89 4.76 hsa-asg-388_st1 hsa-asg-388_st111.05 8.93 9.43 7.28 8.68 9.28 9.11 7.66 7.82 6.83 hsa-asg-13083_st1hsa-asg-13083_st1 10.46 8.14 9.03 6.45 8.11 8.52 8.71 6.85 7.26 6.66hsa-asg-11235_st2 hsa-asg-11235_st2 7.98 6.32 6.74 3.90 5.34 5.55 5.603.75 5.09 5.20 hsa-miR-424 hsa-miR-424_st1 8.38 5.79 6.11 5.53 5.91 5.753.43 5.80 5.70 7.21 hsa-miR-450 hsa-asg-14228_st2 5.46 3.53 2.13 2.402.98 2.49 3.08 2.90 2.89 2.71 hsa-miR-450 hsa-miR-450_st2 6.15 4.22 2.672.84 3.89 3.59 3.97 4.18 4.44 4.04 hsa-asg-1898_st1 hsa-asg-1898_st18.18 5.61 6.38 4.42 5.74 6.01 6.65 4.31 5.55 5.00 hsa-asg-8542_st2hsa-asg-8542_st2 11.32 9.38 10.13 8.18 8.70 8.93 9.60 7.50 7.92 7.64hsa-asg-13559_st2 hsa-asg-13559_st2 8.91 7.01 7.56 5.01 6.55 6.95 7.235.41 5.41 5.22 hsa-asg-12356_st1 hsa-asg-12356_st1 10.05 7.94 8.57 5.857.74 8.64 8.25 6.82 7.01 6.01 hsa-asg-12996_st1 hsa-asg-12996_st1 8.566.75 7.50 5.09 5.79 6.82 6.55 5.07 5.16 4.76 hsa-asg-9547_st2hsa-asg-9547_st2 6.58 3.53 4.90 3.78 3.32 3.40 3.85 3.38 4.25 5.32hsa-asg-4978_st2 hsa-asg-4978_st2 5.07 1.63 2.83 2.84 1.69 1.98 1.952.77 2.58 4.13 hsa-asg-3998_st1 hsa-asg-3998_st1 11.31 8.62 8.53 8.709.41 8.74 8.32 9.20 8.74 8.47 hsa-asg-8683_st1 hsa-asg-8683_st1 9.016.85 7.08 5.69 6.74 7.33 7.60 5.27 5.81 5.86 hsa-asg-378_st1hsa-asg-378_st1 5.24 2.22 2.67 2.15 4.70 2.79 2.94 1.09 2.74 3.06hsa-asg-3009_st1 hsa-asg-3009_st1 7.28 5.17 5.57 4.34 5.20 5.40 5.003.48 4.41 4.17 hsa-asg-6139_st2 hsa-asg-6139_st2 4.83 3.18 2.67 1.631.48 2.99 2.14 1.78 2.41 2.51 hsa-asg-2424_st1 hsa-asg-2424_st1 4.362.22 1.15 1.12 1.79 1.27 1.86 2.62 1.66 2.89 hsa-asg-9453_st2hsa-asg-9453_st2 9.45 7.57 8.10 6.21 6.88 7.39 7.53 6.23 6.75 5.76hsa-asg-13387_st1 hsa-asg-13387_st1 6.85 5.34 5.46 3.30 3.75 3.93 5.433.91 4.82 3.14 hsa-asg-1273_st2 hsa-asg-1273_st2 9.67 7.75 8.42 6.197.31 8.12 8.13 6.46 6.65 5.47 hsa-asg-4903_st2 hsa-asg-4903_st2 9.958.51 8.25 6.62 7.71 7.98 8.37 6.85 7.07 5.67 hsa-asg-11698_st2hsa-asg-11698_st2 10.49 8.56 9.32 6.82 7.99 8.60 9.03 7.39 7.89 6.36hsa-asg-8293_st2 hsa-asg-8293_st2 8.96 7.00 7.28 4.93 6.25 7.31 7.085.89 6.49 5.97 hsa-asg-7472_st2 hsa-asg-7472_st2 4.45 1.63 2.13 1.892.00 2.86 1.76 3.57 0.92 0.93 hsa-asg-12289_st1 hsa-asg-12289_st1 11.499.46 9.97 8.18 9.09 9.82 9.68 8.23 8.88 7.88 hsa-asg-1270_st2hsa-asg-1270_st2 6.10 4.22 3.58 1.89 4.06 4.54 4.83 3.03 3.29 3.22hsa-asg-1751_st2 hsa-asg-1751_st2 10.38 8.85 9.28 7.18 7.91 8.35 8.816.89 7.41 6.59 hsa-asg-6948_st2 hsa-asg-6948_st2 9.98 8.49 8.47 6.627.52 8.31 8.11 6.73 6.80 6.68 hsa-asg-3933_st1 hsa-asg-3933_st1 9.377.72 7.86 5.84 7.23 7.63 7.91 5.91 6.65 5.54 hsa-miR-422ahsa-miR-422a_st1 11.64 9.09 8.97 9.17 9.80 8.95 8.80 9.67 9.22 9.12hsa-asg-8541_st1 hsa-asg-8541_st1 10.88 9.14 9.54 7.73 8.56 8.78 8.857.82 7.83 7.89 hsa-asg-3230_st1 hsa-asg-3230_st1 9.02 7.15 7.96 5.356.84 6.81 7.35 6.04 5.66 6.22 hsa-asg-7020_st1 hsa-asg-7020_st1 4.411.63 2.32 1.89 1.59 1.53 2.32 1.96 2.58 2.07 hsa-asg-6813_st2hsa-asg-6813_st2 6.83 4.41 5.72 4.11 3.60 5.02 5.25 3.48 3.71 4.51hsa-asg-3742_st1 hsa-asg-3742_st1 6.44 3.83 4.90 3.66 4.25 4.84 3.433.57 4.19 3.62 hsa-asg-11802_st1 hsa-asg-11802_st1 9.39 7.71 8.25 6.017.20 7.73 7.92 5.85 6.37 5.84 hsa-asg-9434_st2 hsa-asg-9434_st2 8.746.68 6.97 5.35 6.60 7.28 6.88 5.90 5.81 5.62 hsa-asg-6277_st2hsa-asg-6277_st2 8.38 6.88 6.93 5.07 5.94 6.46 6.96 4.92 6.32 4.54hsa-asg-7527_st2 hsa-asg-7527_st2 6.58 4.59 5.00 2.74 3.12 4.92 4.814.42 4.01 4.25 hsa-asg-9382_st2 hsa-asg-9382_st2 8.77 6.79 7.25 5.666.65 6.89 7.40 5.78 5.78 5.38 hsa-asg-1619_st2 hsa-asg-1619_st2 5.664.35 3.24 2.84 4.80 3.12 2.80 2.31 3.03 3.22 hsa-asg-960_st1hsa-asg-960_st1 10.15 8.36 8.91 6.34 8.10 8.38 8.52 7.02 7.41 7.16hsa-asg-12526_st1 hsa-asg-12526_st1 6.39 4.29 5.16 3.13 3.85 3.18 5.213.15 4.73 3.68 hsa-asg-8305_st2 hsa-asg-8305_st2 9.05 7.08 7.55 5.436.98 7.47 7.34 5.84 6.92 5.74 hsa-cand443_st2 hsa-cand443_st2 6.93 3.915.10 3.90 4.48 5.73 5.21 4.84 3.80 4.29 hsa-asg-6068_st2hsa-asg-6068_st2 7.58 5.79 5.86 4.93 5.24 6.08 5.98 4.12 4.89 4.29hsa-asg-12016_st2 hsa-asg-12016_st2 4.79 3.18 2.67 1.63 2.98 1.71 1.571.96 2.41 4.04 hsa-asg-2314_st1 hsa-asg-2314_st1 8.46 6.19 7.09 5.216.42 6.25 6.53 5.98 5.47 6.11 hsa-asg-9514_st1 hsa-asg-9514_st1 5.752.58 4.50 3.21 2.48 4.33 3.63 4.18 2.58 3.36 hsa-asg-11320_st2hsa-asg-11320_st2 6.52 4.88 4.44 4.11 4.10 4.36 4.05 3.83 3.97 4.21hsa-asg-13878_st2 hsa-asg-13878_st2 5.51 2.90 3.48 2.84 4.82 4.24 3.323.03 2.41 1.83 hsa-asg-2195_st1 hsa-asg-2195_st1 11.52 9.78 10.13 8.179.47 10.16 10.1 8.63 8.95 7.80 hsa-asg-3614_st1 hsa-asg-3614_st1 6.764.79 5.36 3.59 4.38 4.00 5.11 3.83 4.73 4.61 hsa-asg-11361_st2hsa-asg-11361_st2 5.65 2.75 3.48 3.38 3.25 3.59 3.32 3.83 2.58 4.29hsa-asg-2580_st1 hsa-asg-2580_st1 9.51 7.63 8.03 6.56 6.91 7.71 7.446.22 6.68 8.07 hsa-asg-6637_st1 hsa-asg-6637_st1 9.33 7.33 8.19 6.256.81 7.42 7.68 6.05 7.04 6.91 hsa-asg-5163_st1 hsa-asg-5163_st1 9.698.27 8.57 6.97 7.10 7.75 8.41 6.49 7.19 6.27 hsa-asg-8174_st1hsa-asg-8174_st1 5.45 3.31 4.03 2.84 2.98 2.86 3.20 2.77 2.74 4.13hsa-asg-9053_st1 hsa-asg-9053_st1 8.09 5.86 6.50 4.78 6.21 6.07 6.864.88 5.90 5.62 hsa-asg-7250_st1 hsa-asg-7250_st1 6.01 4.00 4.83 2.523.32 4.59 3.97 3.15 4.41 3.14 hsa-asg-3545_st1 hsa-asg-3545_st1 9.497.20 8.47 6.69 6.99 7.91 8.05 6.21 7.08 6.81 hsa-asg-3672_st1hsa-asg-3672_st1 7.40 5.34 6.49 4.16 5.36 5.72 5.99 4.57 4.47 4.48hsa-asg-3912_st1 hsa-asg-3912_st1 4.50 1.63 2.50 2.84 1.59 2.16 1.212.90 2.14 3.50 hsa-asg-9830_st2 hsa-asg-9830_st2 4.88 3.05 2.50 1.892.20 2.16 2.23 3.48 2.23 4.21 hsa-asg-13741_st2 hsa-asg-13741_st2 4.160.15 2.50 1.89 2.57 1.89 2.49 1.42 1.46 3.06 hsa-asg-11626_st1hsa-asg-11626_st1 9.10 7.37 7.81 6.18 7.35 7.77 7.30 6.35 6.21 5.77hsa-asg-9009_st1 hsa-asg-9009_st1 6.80 5.01 5.52 4.16 4.48 4.41 5.204.92 3.66 3.99 hsa-asg-7439_st1 hsa-asg-7439_st1 7.26 4.15 6.57 3.904.94 5.03 6.06 4.31 4.93 5.70 hsa-asg-10360_st1 hsa-asg-10360_st1 5.483.42 3.36 3.04 4.65 3.68 3.20 3.15 2.41 2.71 hsa-asg-4140_st2hsa-asg-4140_st2 5.77 3.42 3.48 3.21 4.35 3.77 3.43 4.12 3.71 2.71hsa-cand266_st1 hsa-cand266_st1 8.56 6.73 7.20 5.49 7.08 6.90 7.03 5.865.97 5.16 hsa-asg-11160_st2 hsa-asg-11160_st2 5.39 2.75 3.11 3.38 3.052.86 2.65 2.47 3.51 5.11 hsa-asg-1040_st2 hsa-asg-1040_st2 7.96 6.506.63 4.25 5.76 6.17 6.28 5.47 5.47 5.49 hsa-asg-2615_st2hsa-asg-2615_st2 10.45 8.47 9.40 7.55 8.26 8.60 9.34 7.15 7.93 7.73hsa-asg-12687_st1 hsa-asg-12687_st1 9.45 7.52 8.13 6.15 7.58 7.91 8.066.36 6.73 7.01 hsa-asg-1794_st1 hsa-asg-1794_st1 4.16 1.83 1.94 2.631.69 2.16 1.03 2.14 1.46 2.89 hsa-asg-466_st1 hsa-asg-466_st1 6.75 4.074.63 2.74 4.96 5.22 4.43 4.84 5.28 5.09 hsa-miR-599 hsa-miR-599_st2 5.573.64 3.48 3.38 4.70 3.40 3.32 4.05 1.85 2.89 hsa-asg-11962_st1hsa-asg-11962_st1 8.92 6.89 7.82 5.76 6.85 7.58 7.40 5.96 6.55 6.01hsa-asg-11993_st1 hsa-asg-11993_st1 9.90 8.00 8.70 6.96 7.98 8.43 8.687.12 7.35 6.54 hsa-cand926_st2 hsa-cand926_st2 5.96 4.29 4.21 2.94 4.213.97 3.77 2.47 4.53 3.89 hsa-asg-3782_st1 hsa-asg-3782_st1 8.24 6.087.03 5.37 6.18 6.72 6.50 5.56 5.90 5.57 hsa-asg-8428_st2hsa-asg-8428_st2 3.92 2.22 1.94 2.63 2.00 2.49 1.03 0.04 2.14 1.60hsa-asg-10414_st1 hsa-asg-10414_st1 9.41 6.96 8.43 5.97 7.02 7.67 7.996.56 7.33 7.55 hsa-asg-6282_st1 hsa-asg-6282_st1 8.14 6.68 6.71 4.586.04 6.63 6.94 5.62 5.92 5.00 hsa-asg-6063_st2 hsa-asg-6063_st2 10.889.14 9.37 8.01 9.04 9.46 9.16 8.43 8.36 7.81 hsa-asg-12441_st1hsa-asg-12441_st1 9.68 7.73 8.87 6.76 7.41 7.72 8.51 6.80 7.66 6.54hsa-asg-11923_st1 hsa-asg-11923_st1 5.63 4.15 3.86 3.38 4.28 4.20 3.432.31 2.41 3.62 hsa-asg-13613_st2 hsa-asg-13613_st2 9.82 8.18 8.61 6.707.93 8.46 8.36 6.98 7.04 7.12 hsa-asg-12376_st2 hsa-asg-12376_st2 7.905.66 6.74 5.60 5.65 5.85 6.56 4.92 5.86 5.28 hsa-asg-8565_st1hsa-asg-8565_st1 8.41 6.55 7.29 5.69 6.16 6.72 6.99 5.62 5.98 5.83hsa-asg-1612_st1 hsa-asg-1612_st1 5.24 2.90 4.31 3.21 3.05 3.35 3.633.48 1.66 2.89 hsa-asg-1277_st1 hsa-asg-1277_st1 10.26 8.56 8.93 7.308.52 8.91 8.80 7.59 7.92 7.21 hsa-asg-3462_st2 hsa-asg-3462_st2 10.338.44 9.26 7.11 8.04 8.52 9.45 7.49 8.74 7.35 hsa-asg-7704_st2hsa-asg-7704_st2 7.45 5.27 7.01 5.04 5.07 5.30 5.92 4.36 5.13 5.40hsa-asg-1596_st2 hsa-asg-1596_st2 6.26 4.41 5.06 4.01 3.19 4.36 4.124.12 4.19 4.37 hsa-asg-11165_st1 hsa-asg-11165_st1 9.38 7.76 8.34 6.767.38 7.99 7.96 6.83 6.92 5.95 hsa-asg-9099_st2 hsa-asg-9099_st2 6.074.00 5.50 3.38 3.75 3.81 5.15 2.90 3.97 3.62 hsa-asg-5413_st2hsa-asg-5413_st2 7.71 5.44 6.76 5.33 5.62 6.37 6.00 4.71 5.30 5.40hsa-asg-11940_st1 hsa-asg-11940_st1 7.59 5.31 6.85 4.42 5.36 5.24 6.335.24 5.69 5.52 hsa-asg-4069_st1 hsa-asg-4069_st1 6.15 4.29 4.88 2.744.44 4.27 4.92 4.12 3.56 3.89 hsa-asg-3646_st1 hsa-asg-3646_st1 6.133.31 5.02 4.01 4.38 3.24 4.65 3.57 3.51 5.24 hsa-asg-8138_st2hsa-asg-8138_st2 7.65 6.41 6.52 4.42 5.36 6.40 6.34 4.80 5.15 5.30hsa-cand899_st2 hsa-cand899_st2 6.07 2.75 4.74 3.30 3.98 4.17 4.77 3.384.22 5.09 hsa-asg-7159_st2 hsa-asg-7159_st2 5.37 3.53 3.24 2.40 3.943.89 3.43 3.98 3.40 2.29 hsa-miR112_st1 hsa-miR112_st1 9.40 7.55 8.236.14 7.20 7.94 8.13 6.58 7.56 7.09 hsa-asg-7654_st2 hsa-asg-7654_st28.80 6.84 7.81 5.73 6.82 7.07 7.78 6.16 6.10 6.80 hsa-asg-7954_st1hsa-asg-7954_st1 9.10 7.73 8.01 6.19 7.36 7.83 7.45 6.15 6.70 6.37hsa-asg-8329_st1 hsa-asg-8329_st1 7.65 6.26 6.52 4.42 5.87 6.14 6.175.49 5.53 4.41 hsa-miR-200a* hsa-miR-200a-_st2 4.72 3.05 2.83 2.15 3.892.57 1.76 2.14 3.17 2.89 hsa-asg-807_st2 hsa-asg-807_st2 6.86 4.15 5.414.54 4.35 4.71 5.52 4.57 4.97 5.54 hsa-asg-12556_st1 hsa-asg-12556_st19.65 8.06 8.67 6.55 7.78 8.32 8.39 6.96 7.29 6.90 hsa-asg-1576_st1hsa-asg-1576_st1 9.51 7.85 8.09 6.65 7.36 8.04 7.46 7.09 7.43 7.63hsa-asg-265_st2 hsa-asg-265_st2 7.96 6.45 7.13 5.14 5.70 5.87 7.03 5.275.77 5.36 hsa-asg-13471_st1 hsa-asg-13471_st1 6.60 4.74 4.71 4.38 5.115.31 5.34 4.36 4.04 3.50 hsa-asg-10055_st1 hsa-asg-10055_st1 3.92 2.030.97 2.15 1.09 1.98 2.65 1.42 2.23 2.89 hsa-cand630_st2 hsa-cand630_st27.06 5.17 6.10 5.26 4.53 4.96 5.64 3.75 5.03 5.26 hsa-asg-4207_st1hsa-asg-4207_st1 6.76 5.09 5.41 4.01 4.82 5.62 5.57 4.52 4.32 3.62hsa-miR-422b hsa-miR-422b_st2 12.69 10.62 10.51 10.71 11.3 10.68 10.310.9 10.7 10.5 hsa-asg-4283_st2 hsa-asg-4283_st2 5.17 3.91 3.48 2.633.80 3.89 2.80 3.48 2.23 2.51 hsa-cand330_st2 hsa-cand330_st2 6.17 4.744.98 3.52 4.70 5.12 4.12 2.62 3.97 3.94 hsa-asg-10478_st2hsa-asg-10478_st2 5.37 3.05 4.03 3.38 2.48 4.38 4.12 3.66 2.74 2.71hsa-asg-3740_st1 hsa-asg-3740_st1 7.07 4.88 6.41 4.68 5.07 5.43 5.883.87 4.85 4.82 hsa-cand852_st2 hsa-cand852_st2 4.64 3.05 1.94 2.84 2.982.24 2.65 2.90 2.05 3.50 hsa-asg-3150_st1 hsa-asg-3150_st1 8.10 6.397.40 5.51 6.17 5.96 6.77 5.67 5.87 5.59 hsa-asg-6339_st1hsa-asg-6339_st1 9.36 7.51 8.13 6.67 7.33 7.99 7.78 6.59 7.56 7.30hsa-asg-11973_st2 hsa-asg-11973_st2 8.62 6.66 7.23 6.67 6.88 7.04 7.016.05 6.53 6.08 hsa-asg-3746_st1 hsa-asg-3746_st1 9.55 8.01 8.78 6.567.51 8.00 8.44 7.08 7.30 6.93 hsa-asg-12440_st2 hsa-asg-12440_st2 6.594.74 4.83 3.84 4.75 4.80 5.23 4.42 4.78 4.54 hsa-asg-3754_st2hsa-asg-3754_st2 6.73 4.97 4.96 4.84 3.44 5.09 5.00 4.84 5.50 4.61hsa-asg-8893_st1 hsa-asg-8893_st1 6.62 4.97 4.92 4.71 4.80 5.44 4.984.71 3.46 4.33 hsa-asg-3728_st1 hsa-asg-3728_st1 8.21 6.64 7.33 5.436.53 6.79 6.90 5.52 5.81 5.68 hsa-asg-2624_st2 hsa-asg-2624_st2 4.643.18 3.24 1.89 3.60 2.57 3.32 1.78 3.17 1.83 hsa-cand805_st2hsa-cand805_st2 10.05 8.23 9.20 7.34 8.24 8.82 8.70 7.66 7.87 7.21hsa-asg-10043_st1 hsa-asg-10043_st1 4.50 3.18 2.50 1.89 3.12 3.29 2.142.90 2.05 2.29 hsa-asg-3406_st1 hsa-asg-3406_st1 5.34 3.73 3.05 3.132.98 3.81 4.08 3.91 2.32 3.99 hsa-asg-7671_st1 hsa-asg-7671_st1 8.156.24 7.39 5.76 6.13 6.82 6.90 5.60 6.17 5.30 hsa-asg-1127_st2hsa-asg-1127_st2 5.43 3.64 4.03 3.52 2.98 3.64 4.19 3.75 2.41 3.73hsa-asg-7228_st1 hsa-asg-7228_st1 7.10 5.79 6.36 4.25 4.59 5.39 5.954.71 4.35 5.59 hsa-asg-4786_st1 hsa-asg-4786_st1 8.72 7.17 7.73 5.666.78 7.35 7.48 6.56 6.77 6.19 hsa-asg-7388_st1 hsa-asg-7388_st1 8.136.51 6.92 5.47 6.19 6.95 6.91 5.90 5.83 5.80 hsa-asg-7579_st2hsa-asg-7579_st2 7.45 5.95 6.66 5.04 5.70 6.45 5.98 4.71 5.20 4.64hsa-miR-517c hsa-miR-517c_st2 4.54 2.90 2.50 3.04 3.60 3.06 3.43 1.422.23 2.07 hsa-asg-12760_st2 hsa-asg-12760_st2 5.58 3.83 4.50 4.01 3.123.35 3.97 4.05 2.58 4.21 hsa-asg-4872_st1 hsa-asg-4872_st1 5.39 3.314.50 3.04 3.85 2.65 4.22 2.62 3.88 3.94 hsa-asg-9273_st2hsa-asg-9273_st2 11.12 9.53 10.06 8.40 9.37 10.18 9.97 8.78 9.20 8.10hsa-asg-9511_st2 hsa-asg-9511_st2 8.51 6.96 7.27 6.18 6.81 7.12 7.025.88 6.36 6.48 hsa-cand441_st2 hsa-cand441_st2 9.14 7.72 8.02 5.93 7.307.92 7.90 6.86 7.44 6.70 hsa-asg-9270_st1 hsa-asg-9270_st1 4.62 2.413.58 2.15 3.44 2.65 3.68 1.42 2.74 3.06 hsa-asg-8193_st2hsa-asg-8193_st2 6.56 5.34 5.87 4.34 4.82 4.44 5.21 3.91 4.63 3.99hsa-asg-9412_st2 hsa-asg-9412_st2 8.78 7.03 7.88 5.85 6.63 7.71 7.756.79 6.24 6.71 hsa-asg-12735_st1 hsa-asg-12735_st1 8.26 6.81 6.64 5.856.49 7.38 7.02 6.36 6.06 5.30 hsa-asg-5241_st1 hsa-asg-5241_st1 4.983.64 3.58 2.63 3.25 3.18 2.49 3.57 2.41 3.73 hsa-asg-6958_st2hsa-asg-6958_st2 6.08 4.97 4.52 3.84 3.55 4.33 4.74 3.57 4.91 3.99hsa-asg-1922_st2 hsa-asg-1922_st2 7.38 5.76 6.06 5.39 5.86 5.60 5.994.88 5.66 4.95 hsa-asg-5149_st2 hsa-asg-5149_st2 6.66 5.31 4.92 4.714.82 5.40 5.21 3.66 4.78 4.90 hsa-asg-5385_st2 hsa-asg-5385_st2 7.595.79 6.49 5.59 5.50 5.73 6.35 5.20 5.66 5.86 hsa-asg-4847_st1hsa-asg-4847_st1 8.59 6.87 7.71 5.94 6.47 7.10 7.37 5.82 6.80 7.10hsa-cand6_st2 hsa-cand6_st2 8.70 7.03 7.79 6.10 6.90 7.17 7.36 6.27 6.437.12 hsa-asg-809_st2 hsa-asg-809_st2 7.41 6.22 6.22 5.14 6.23 6.11 6.104.62 5.62 4.51 hsa-asg-748_st1 hsa-asg-748_st1 6.45 5.01 5.97 3.90 3.945.11 5.00 4.18 4.55 4.44 hsa-asg-1187_st2 hsa-asg-1187_st2 7.41 5.616.40 4.84 5.66 6.61 6.18 4.88 5.44 5.22 hsa-asg-9248_st2hsa-asg-9248_st2 9.91 8.57 8.95 7.30 8.04 8.83 8.94 7.43 8.10 7.13hsa-asg-4759_st1 hsa-asg-4759_st1 8.57 7.03 7.97 5.85 6.74 7.35 7.545.92 6.42 6.54 hsa-asg-5289_st2 hsa-asg-5289_st2 7.80 6.05 7.00 5.395.67 6.17 6.84 5.14 6.79 5.38 hsa-asg-6129_st2 hsa-asg-6129_st2 8.646.85 7.52 6.94 7.11 7.08 7.60 6.69 5.78 6.43 hsa-asg-13997_st2hsa-asg-13997_st2 4.26 3.05 3.24 2.15 2.98 2.16 2.32 1.25 3.17 2.29hsa-asg-8552_st2 hsa-asg-8552_st2 12.26 10.96 11.28 9.81 10.5 11.21 11.09.76 10.1 9.89 hsa-asg-3030_st2 hsa-asg-3030_st2 5.45 3.05 4.44 3.213.89 4.47 3.32 3.48 3.61 3.94 hsa-asg-7278_st1 hsa-asg-7278_st1 6.755.20 5.73 4.34 4.92 5.28 5.54 3.98 4.73 5.42 hsa-asg-9165_st1hsa-asg-9165_st1 8.36 6.05 7.45 5.53 6.41 7.48 7.32 6.21 6.83 6.35hsa-asg-6149_st2 hsa-asg-6149_st2 8.70 7.32 7.46 6.24 6.93 7.73 7.286.28 7.62 5.87 hsa-asg-2637_st1 hsa-asg-2637_st1 5.86 4.35 4.14 4.964.53 4.24 3.38 3.38 3.84 4.33 hsa-asg-4814_st1 hsa-asg-4814_st1 7.966.51 7.06 5.35 6.03 6.30 6.85 5.41 6.42 6.22 hsa-asg-9287_st2hsa-asg-9287_st2 7.34 5.66 6.18 4.71 5.39 6.63 5.97 4.67 5.83 5.52hsa-asg-14051_st2 hsa-asg-14051_st2 8.29 6.09 7.27 5.94 5.91 6.88 7.076.09 6.95 6.96 hsa-asg-12515_st2 hsa-asg-12515_st2 5.69 4.00 4.25 3.044.10 4.57 4.32 3.66 4.12 3.73 hsa-asg-1293_st2 hsa-asg-1293_st2 7.756.39 6.83 5.59 6.19 6.21 6.58 5.49 5.76 5.34 hsa-asg-7471_st1hsa-asg-7471_st1 7.36 5.58 5.67 5.68 6.74 5.67 5.25 5.24 4.97 6.07hsa-asg-8675_st1 hsa-asg-8675_st1 7.40 5.69 6.76 5.66 5.53 5.51 6.204.71 5.50 5.65 hsa-asg-13999_st1 hsa-asg-13999_st1 9.05 8.18 8.00 7.277.18 7.59 7.75 6.52 7.19 6.44 hsa-asg-10772_st1 hsa-asg-10772_st1 7.906.44 6.77 6.14 6.74 5.54 6.64 5.58 6.00 5.97 hsa-asg-12395_st2hsa-asg-12395_st2 7.33 4.93 5.80 4.78 5.96 6.82 6.08 5.20 5.41 5.77hsa-asg-4703_st2 hsa-asg-4703_st2 6.93 5.34 6.13 4.61 4.87 5.70 5.844.76 5.23 4.70 hsa-asg-9507_st1 hsa-asg-9507_st1 10.82 9.41 9.98 8.359.28 9.81 9.72 8.49 8.96 8.23 hsa-asg-7336_st1 hsa-asg-7336_st1 7.245.27 6.28 5.39 5.53 6.00 6.39 4.84 5.58 4.67 hsa-asg-9262_st1hsa-asg-9262_st1 6.14 4.59 4.28 3.96 5.74 4.71 4.52 3.75 4.08 4.48hsa-asg-12298_st1 hsa-asg-12298_st1 5.69 4.64 5.08 4.06 3.89 3.81 4.153.27 3.35 3.89 hsa-asg-5176_st2 hsa-asg-5176_st2 4.13 2.22 3.24 2.151.69 2.72 3.08 1.78 2.58 2.71 hsa-asg-11714_st2 hsa-asg-11714_st2 9.918.92 9.15 7.34 8.04 8.35 9.06 7.49 8.22 7.70 hsa-asg-3345_st1hsa-asg-3345_st1 9.45 8.09 8.28 6.99 7.48 8.27 7.87 6.91 8.09 8.28hsa-asg-12625_st1 hsa-asg-12625_st1 5.44 4.22 5.33 3.59 3.44 3.18 4.053.57 3.46 3.29 hsa-asg-12014_st2 hsa-asg-12014_st2 9.89 8.54 8.87 7.558.09 8.75 8.91 7.61 8.28 7.77 hsa-asg-124_st1 hsa-asg-124_st1 6.65 4.885.28 4.30 4.56 5.70 5.34 5.27 4.75 5.11 hsa-asg-4840_st1hsa-asg-4840_st1 6.42 5.44 5.02 3.52 5.28 5.40 4.98 4.62 4.55 4.37hsa-asg-10971_st2 hsa-asg-10971_st2 7.63 6.22 6.90 5.39 5.96 6.17 5.985.98 6.43 5.04 hsa-asg-9879_st1 hsa-asg-9879_st1 4.81 2.22 4.25 2.633.32 3.93 3.14 2.77 3.40 3.06 hsa-asg-11795_st2 hsa-asg-11795_st2 7.055.09 6.45 5.45 4.92 5.99 5.72 4.76 5.75 4.82 hsa-asg-3952_st1hsa-asg-3952_st1 10.23 8.66 9.42 7.48 8.39 8.75 9.22 7.90 8.70 9.03hsa-miR-576 hsa-miR-576_st2 3.92 2.22 2.83 3.04 1.69 1.98 1.95 1.96 2.053.06 hsa-asg-14025_st1 hsa-asg-14025_st1 7.65 6.37 6.65 5.26 6.02 6.736.29 5.33 6.06 5.62 hsa-asg-13135_st1 hsa-asg-13135_st1 6.72 5.61 5.615.24 4.89 4.76 4.70 5.14 4.44 5.57 hsa-asg-8872_st1 hsa-asg-8872_st18.77 7.85 7.97 6.91 6.99 7.39 7.83 6.21 7.06 6.34 hsa-asg-11391_st1hsa-asg-11391_st1 3.72 1.43 1.54 2.40 2.00 3.40 1.86 2.62 2.23 1.60hsa-asg-8163_st1 hsa-asg-8163_st1 10.45 8.54 9.72 8.47 8.38 8.99 9.588.08 9.61 8.26 hsa-asg-12777_st2 hsa-asg-12777_st2 7.51 5.86 6.42 4.935.47 5.83 6.45 6.12 6.01 6.14 hsa-asg-10589_st1 hsa-asg-10589_st1 3.721.05 1.64 3.04 2.66 2.49 1.76 2.39 1.85 2.29 hsa-asg-1893_st2hsa-asg-1893_st2 9.19 7.54 8.49 6.67 7.48 8.09 8.41 6.84 7.38 7.61hsa-asg-8173_st1 hsa-asg-8173_st1 8.38 6.79 7.31 5.41 6.89 7.56 7.276.36 6.65 6.97 hsa-asg-5265_st1 hsa-asg-5265_st1 8.11 6.74 7.06 5.826.30 7.25 6.97 6.30 6.40 5.96 hsa-asg-6961_st2 hsa-asg-6961_st2 6.765.84 6.10 5.31 5.11 4.66 4.67 4.71 4.78 5.56 hsa-asg-12150_st1hsa-asg-12150_st1 9.39 7.95 8.76 7.20 7.43 8.09 8.33 6.75 7.97 7.97hsa-asg-6618_st2 hsa-asg-6618_st2 6.93 5.47 5.94 4.50 5.31 5.71 6.064.92 5.18 5.22 hsa-asg-14230_st1 hsa-asg-14230_st1 5.37 4.29 3.68 4.384.56 3.29 3.43 2.77 3.93 4.04 hsa-asg-938_st2 hsa-asg-938_st2 8.05 7.107.14 6.68 5.44 6.62 6.87 5.64 6.70 6.38 hsa-asg-9515_st1hsa-asg-9515_st1 7.04 5.05 5.88 5.09 5.36 5.72 6.20 5.10 5.40 5.79hsa-asg-9500_st1 hsa-asg-9500_st1 5.44 4.59 4.58 3.59 3.80 3.55 4.083.15 4.15 3.68 hsa-miR223_st2 hsa-miR223_st2 6.90 5.50 6.08 4.96 4.705.14 6.01 5.67 5.28 5.09 hsa-miR-498 hsa-miR-498_st1 7.01 5.71 6.31 5.244.87 5.87 6.13 4.76 5.40 5.11 hsa-asg-14217_st1 hsa-asg-14217_st1 6.875.13 5.87 5.39 4.59 4.96 5.69 4.52 5.74 6.34 hsa-asg-1227_st1hsa-asg-1227_st1 5.87 5.09 4.85 3.52 4.17 5.03 4.70 4.12 4.38 3.50hsa-asg-8490_st1 hsa-asg-8490_st1 7.51 6.24 6.44 5.85 5.81 6.08 5.935.30 6.27 6.28 hsa-asg-8639_st2 hsa-asg-8639_st2 10.40 8.90 9.82 8.088.69 9.38 9.14 8.32 9.06 8.84 hsa-asg-6500_st1 hsa-asg-6500_st1 6.254.74 4.81 4.68 4.02 5.11 5.31 3.66 5.09 5.47 hsa-asg-1715_st2hsa-asg-1715_st2 4.72 3.53 3.78 3.21 2.30 2.57 3.20 4.18 3.40 3.06hsa-asg-9334_st2 hsa-asg-9334_st2 8.18 6.32 7.60 6.15 6.41 6.92 7.316.22 6.60 6.85 hsa-asg-8896_st2 hsa-asg-8896_st2 6.24 3.73 5.27 4.165.11 4.84 4.52 5.36 4.95 5.09 hsa-asg-10175_st2 hsa-asg-10175_st2 3.642.41 2.50 1.89 2.00 1.80 2.87 2.31 1.46 2.51 hsa-miR-503 hsa-miR-503_st18.06 6.88 7.17 7.38 6.60 6.08 6.45 6.39 6.60 5.99 hsa-asg-13197_st1hsa-asg-13197_st1 4.31 2.41 2.67 3.38 3.25 2.16 2.94 2.47 3.03 3.50hsa-asg-1168_st1 hsa-asg-1168_st1 7.45 6.15 6.80 5.26 5.78 6.54 6.645.57 5.55 5.74 hsa-asg-5047_st1 hsa-asg-5047_st1 6.06 4.47 5.47 4.114.28 5.21 4.88 3.57 4.80 4.76 hsa-asg-773_st1 hsa-asg-773_st1 8.32 7.217.41 6.30 6.35 7.33 6.73 6.33 7.34 6.93 hsa-miR-379 hsa-miR-379_st1 8.016.51 6.37 7.12 6.96 7.01 6.54 6.33 6.13 6.20 hsa-asg-2041_st1hsa-asg-2041_st1 7.02 5.93 6.09 5.37 5.59 5.87 5.70 5.10 5.75 4.87hsa-asg-9253_st2 hsa-asg-9253_st2 3.89 3.31 2.67 1.89 2.30 3.12 2.322.62 1.85 2.07 hsa-asg-8636_st2 hsa-asg-8636_st2 7.56 5.84 6.86 5.435.53 6.21 6.65 5.64 6.60 6.45 hsa-asg-1903_st2 hsa-asg-1903_st2 10.289.18 9.22 8.31 9.24 9.53 9.31 8.54 8.49 8.09 hsa-asg-13334_st2hsa-asg-13334_st2 4.38 3.05 3.36 2.15 3.25 3.45 2.80 3.27 2.89 2.71hsa-miR165_st1 hsa-miR165_st1 6.60 5.66 5.71 5.31 5.17 5.40 5.43 4.714.82 4.73 hsa-asg-2862_st1 hsa-asg-2862_st1 8.19 6.32 7.27 7.50 6.846.71 6.93 6.57 6.67 6.53 hsa-asg-9050_st1 hsa-asg-9050_st1 8.86 8.138.10 6.94 7.53 7.52 7.64 6.69 7.55 7.29 hsa-asg-8946_st1hsa-asg-8946_st1 5.82 4.22 4.98 4.01 3.85 4.71 5.21 3.57 4.61 4.87hsa-asg-9209_st2 hsa-asg-9209_st2 7.75 6.13 6.97 5.97 6.34 6.48 6.845.74 6.55 6.38 hsa-asg-956_st2 hsa-asg-956_st2 4.31 3.53 3.58 2.84 2.482.33 3.32 2.47 2.58 3.36 hsa-asg-8188_st1 hsa-asg-8188_st1 8.38 7.378.04 6.15 6.88 7.25 7.06 6.32 7.08 6.98 hsa-asg-2849_st2hsa-asg-2849_st2 4.74 3.31 4.11 2.40 2.82 3.50 4.12 3.27 3.40 3.50hsa-asg-13350_st2 hsa-asg-13350_st2 9.92 9.28 9.03 8.32 8.83 8.59 8.828.28 8.26 8.05 hsa-asg-9687_st1 hsa-asg-9687_st1 5.46 4.74 4.76 3.904.65 3.85 4.19 4.05 3.71 3.50 hsa-asg-6924_st1 hsa-asg-6924_st1 7.856.53 7.33 6.10 7.10 6.12 6.92 6.35 5.96 6.54 hsa-miR-491 hsa-miR-491_st16.77 5.24 6.12 5.04 5.61 5.51 5.70 5.12 5.48 5.44 hsa-asg-6890_st1hsa-asg-6890_st1 5.57 4.41 4.47 4.01 4.59 3.85 4.32 4.15 4.25 4.44hsa-asg-10291_st2 hsa-asg-10291_st2 5.16 4.15 4.52 4.06 4.02 3.59 2.943.83 3.46 4.25 hsa-cand960_st2 hsa-cand960_st2 6.15 4.97 5.80 4.25 4.675.28 5.04 4.76 4.63 4.41 hsa-asg-961_st1 hsa-asg-961_st1 5.19 3.73 4.503.21 3.75 3.68 4.85 3.91 3.97 3.50 hsa-asg-3126_st1 hsa-asg-3126_st15.13 3.31 4.44 4.30 3.05 3.85 4.08 3.48 3.97 4.21 hsa-asg-8039_st2hsa-asg-8039_st2 4.85 3.31 4.28 3.13 3.44 2.79 3.43 3.98 4.08 3.79hsa-asg-11481_st2 hsa-asg-11481_st2 4.95 3.18 4.03 3.21 3.75 3.18 4.013.91 4.04 3.84 hsa-asg-10065_st1 hsa-asg-10065_st1 5.37 4.47 4.31 3.664.70 4.44 3.63 4.05 3.80 3.94 hsa-asg-1319_st2 hsa-asg-1319_st2 6.985.50 6.23 5.26 5.74 6.43 6.09 5.38 5.87 5.09 hsa-asg-7170_st2hsa-asg-7170_st2 5.16 4.22 3.78 3.21 4.10 3.81 4.70 3.48 4.44 3.50hsa-asg-1689_st2 hsa-asg-1689_st2 6.65 5.58 5.96 5.21 5.13 5.54 5.434.76 5.32 5.77 hsa-asg-9491_st1 hsa-asg-9491_st1 5.79 4.53 4.96 3.724.82 4.69 4.96 4.12 4.95 4.17 hsa-asg-381_st1 hsa-asg-381_st1 4.54 3.053.58 3.04 3.66 3.81 3.32 3.48 3.29 2.51 hsa-asg-8253_st2hsa-asg-8253_st2 10.93 9.67 10.27 8.79 9.49 10.36 10.0 9.21 9.70 9.78hsa-cand753_st1 hsa-cand753_st1 6.59 5.55 5.86 4.93 5.18 5.39 5.79 4.625.33 5.61 hsa-asg-2542_st1 hsa-asg-2542_st1 6.08 4.69 4.83 3.72 4.625.16 5.21 4.96 5.28 5.30 hsa-asg-125_st1 hsa-asg-125_st1 9.30 8.40 8.457.21 7.84 8.63 8.33 7.89 8.06 7.94 hsa-asg-3722_st1 hsa-asg-3722_st110.11 9.12 9.51 9.24 8.61 8.85 8.99 8.29 9.00 8.68 hsa-asg-4208_st1hsa-asg-4208_st1 4.85 3.42 3.95 3.90 3.89 3.06 3.63 3.48 4.25 3.36hsa-asg-9411_st1 hsa-asg-9411_st1 6.28 5.66 4.61 5.33 5.36 5.05 4.835.03 4.71 5.24 hsa-asg-11900_st1 hsa-asg-11900_st1 6.87 5.34 6.05 5.375.50 6.18 5.07 6.12 6.22 5.36 hsa-asg-3224_st1 hsa-asg-3224_st1 5.344.79 3.78 4.46 4.48 4.38 4.32 3.48 3.61 4.29 hsa-cand659_st1hsa-cand659_st1 8.94 7.82 7.93 7.34 7.90 8.06 8.08 7.49 7.61 7.81hsa-asg-12874_st1 hsa-asg-12874_st1 5.56 4.00 4.18 4.01 4.85 4.57 4.834.18 4.50 4.64 hsa-asg-12216_st1 hsa-asg-12216_st1 4.81 3.73 4.03 3.523.60 4.14 3.72 3.27 3.71 3.50 hsa-asg-917_st2 hsa-asg-917_st2 6.24 5.695.25 4.61 4.89 5.89 4.79 5.00 4.85 5.20 hsa-asg-6845_st2hsa-asg-6845_st2 3.79 2.58 3.05 2.40 3.12 2.65 2.80 2.22 2.41 3.06hsa-asg-1896_st2 hsa-asg-1896_st2 4.21 3.42 3.36 2.40 3.19 3.50 3.632.90 2.50 3.22 hsa-cand764_st1 hsa-cand764_st1 6.92 5.86 6.04 5.55 5.736.35 6.04 5.03 6.36 5.62 hsa-asg-4118_st1 hsa-asg-4118_st1 7.84 6.667.09 7.12 7.22 6.70 7.13 6.50 6.41 6.18 hsa-asg-4836_st2hsa-asg-4836_st2 6.62 5.58 6.19 5.41 5.29 5.65 5.84 5.07 5.65 5.54hsa-miR203_st1 hsa-miR203_st1 7.11 6.05 6.89 5.91 5.49 5.97 6.40 5.746.17 6.28 hsa-miR-496 hsa-miR-496_st1 3.12 2.41 2.67 1.89 2.30 1.62 2.492.14 1.66 1.83 LNneg Micro RNA M SD % ttest log2Diff Fold hsa-miR-2065.32 2.31 44 8.20E−03 −8.51 364.0 hsa-miR-133b 6.53 1.79 67 9.54E−03−6.39 83.8 hsa-miR-499 4.87 0.85 22 1.12E−04 −6.30 78.6 hsa-miR-499 4.860.74 22 4.95E−05 −6.13 70.0 hsa-miR-133a 7.59 1.47 89 5.83E−03 −5.7654.2 hsa-asg-4915_st1 2.96 0.81 0 3.07E−04 −5.16 35.8 hsa-miR-429 2.861.14 0 2.71E−03 −5.13 35.0 hsa-miR-200b 7.68 1.27 100 6.53E−03 −4.8829.4 hsa-asg-2027_st1 3.94 1.01 0 2.01E−03 −4.79 27.6 hsa-miR-200a 5.541.15 67 6.27E−03 −4.45 21.8 hsa-miR-200c 9.05 1.21 100 1.11E−02 −4.2018.4 hsa-asg-2399_st2 2.52 0.67 0 4.73E−04 −3.99 15.9 hsa-miR-605 2.521.03 0 6.65E−03 −3.95 15.5 hsa-asg-14153_st2 2.73 0.85 0 2.49E−03 −3.8914.9 hsa-asg-11715_st2 1.90 0.74 0 1.32E−03 −3.76 13.6 hsa-asg-2484_st22.57 0.74 0 1.51E−03 −3.70 13.0 hsa-asg-3983_st1 5.77 0.80 0 2.36E−03−3.70 13.0 hsa-asg-6283_st1 4.19 1.50 11 4.76E−02 −3.69 12.9 hsa-miR-3686.25 1.40 67 3.87E−02 −3.65 12.6 hsa-asg-4209_st2 4.22 0.88 0 4.42E−03−3.64 12.5 hsa-miR-381 3.08 0.91 0 5.40E−03 −3.64 12.5 hsa-miR-203 6.771.38 78 3.78E−02 −3.63 12.4 hsa-asg-10309_st1 4.22 1.45 33 4.63E−02−3.59 12.0 hsa-asg-2345_st2 2.35 0.76 0 2.08E−03 −3.59 12.0hsa-asg-10393_st1 2.66 0.74 0 1.80E−03 −3.59 12.0 hsa-cand14_st1 2.200.65 0 8.78E−04 −3.55 11.7 hsa-asg-1375_st1 6.11 0.95 56 7.84E−03 −3.5111.4 hsa-asg-12172_st2 6.54 1.16 67 2.11E−02 −3.50 11.3 hsa-asg-6178_st22.29 0.93 0 7.89E−03 −3.46 11.0 hsa-miR-539 2.19 0.63 0 8.76E−04 −3.4210.7 hsa-miR-539 2.52 0.63 0 1.37E−03 −3.19 9.2 hsa-asg-3473_st1 5.851.16 0 2.35E−02 −3.42 10.7 hsa-asg-6623_st2 5.99 0.93 56 8.40E−03 −3.3910.5 hsa-asg-3081_st1 3.99 1.21 11 2.92E−02 −3.38 10.4 hsa-miR-141 4.160.69 0 1.86E−03 −3.33 10.0 hsa-asg-12142_st1 2.29 0.70 0 1.93E−03 −3.3210.0 hsa-asg-1020_st2 5.92 1.24 67 3.53E−02 −3.29 9.8 hsa-asg-3743_st13.19 0.68 0 2.01E−03 −3.24 9.4 hsa-asg-1826_st2 2.72 0.59 0 8.28E−04−3.22 9.3 hsa-asg-4116_st2 6.02 1.00 56 1.53E−02 −3.22 9.3hsa-asg-924_st1 3.38 0.89 0 9.53E−03 −3.20 9.2 hsa-asg-555_st1 6.60 0.8978 9.30E−03 −3.19 9.1 hsa-asg-9920_st1 7.32 0.85 44 7.94E−03 −3.14 8.8hsa-asg-20_st1 2.76 1.20 0 3.85E−02 −3.13 8.8 hsa-asg-1296_st1 3.24 0.850 8.28E−03 −3.13 8.7 hsa-asg-10250_st1 2.04 0.74 0 4.11E−03 −3.11 8.7hsa-asg-4037_st1 2.51 0.58 0 9.45E−04 −3.11 8.7 hsa-asg-8242_st2 3.111.16 0 3.41E−02 −3.11 8.6 hsa-asg-1699_st2 7.25 1.16 56 3.46E−02 −3.108.6 hsa-asg-7637_st2 3.78 0.77 0 5.41E−03 −3.06 8.3 hsa-asg-1765_st12.56 0.55 0 8.03E−04 −3.03 8.2 hsa-asg-9512_st1 6.23 1.01 11 2.21E−02−3.02 8.1 hsa-asg-12139_st2 2.32 0.59 0 1.25E−03 −3.02 8.1hsa-asg-7751_st2 3.42 1.17 0 4.07E−02 −3.00 8.0 hsa-asg-7292_st1 7.740.76 100 5.83E−03 −2.99 7.9 hsa-asg-128_st2 4.58 0.35 0 4.32E−05 −2.987.9 hsa-asg-7550_st1 3.40 0.83 0 9.24E−03 −2.97 7.8 hsa-miR-376a 4.341.02 0 2.52E−02 −2.95 7.7 hsa-miR-376a 4.83 0.75 11 6.93E−03 −2.86 7.3hsa-miR-376a 3.57 0.86 0 1.72E−02 −2.73 6.6 hsa-asg-10131_st2 3.22 0.790 8.07E−03 −2.91 7.5 hsa-asg-13070_st1 2.54 0.79 0 8.55E−03 −2.89 7.4hsa-miR-95 9.00 0.80 100 8.89E−03 −2.89 7.4 hsa-asg-4864_st1 6.35 1.1478 4.41E−02 −2.87 7.3 hsa-asg-3597_st2 6.60 0.88 78 1.49E−02 −2.87 7.3hsa-asg-9201_st2 5.48 0.87 11 1.43E−02 −2.85 7.2 hsa-asg-8079_st1 4.710.91 11 1.76E−02 −2.85 7.2 hsa-miR-135b 2.57 0.55 0 1.19E−03 −2.85 7.2hsa-miR-135b 3.18 0.75 0 2.63E−02 −2.16 4.5 hsa-asg-13888_st2 2.24 0.600 2.02E−03 −2.83 7.1 hsa-asg-11777_st1 2.48 0.60 0 1.97E−03 −2.83 7.1hsa-miR-138 5.62 0.80 33 1.09E−02 −2.78 6.9 hsa-asg-10080_st1 2.31 0.480 5.84E−04 −2.77 6.8 hsa-asg-5119_st1 2.49 0.55 0 1.46E−03 −2.77 6.8hsa-asg-9082_st1 2.99 0.89 0 1.88E−02 −2.76 6.8 hsa-asg-5728_st2 6.350.64 67 3.42E−03 −2.76 6.8 hsa-asg-5189_st2 7.86 1.00 100 3.13E−02 −2.746.7 hsa-asg-677_st2 4.04 0.72 0 7.13E−03 −2.74 6.7 hsa-asg-13077_st14.76 0.95 44 2.65E−02 −2.73 6.6 hsa-asg-3822_st2 2.72 0.99 0 3.11E−02−2.72 6.6 hsa-asg-388_st1 8.33 0.95 100 2.69E−02 −2.72 6.6hsa-asg-13083_st1 7.75 0.96 78 2.79E−02 −2.71 6.5 hsa-asg-11235_st2 5.280.98 22 3.09E−02 −2.70 6.5 hsa-miR-424 5.69 0.98 89 3.18E−02 −2.68 6.4hsa-miR-450 2.79 0.42 0 2.89E−04 −2.67 6.4 hsa-miR-450 3.76 0.62 06.27E−03 −2.39 5.3 hsa-asg-1898_st1 5.52 0.81 11 1.43E−02 −2.66 6.3hsa-asg-8542_st2 8.66 0.92 44 2.62E−02 −2.65 6.3 hsa-asg-13559_st2 6.260.99 56 3.48E−02 −2.65 6.3 hsa-asg-12356_st1 7.43 1.05 78 4.57E−02 −2.626.2 hsa-asg-12996_st1 5.94 0.98 56 3.55E−02 −2.62 6.1 hsa-asg-9547_st23.97 0.72 0 8.69E−03 −2.61 6.1 hsa-asg-4978_st2 2.49 0.79 0 1.43E−02−2.58 6.0 hsa-asg-3998_st1 8.75 0.35 100 1.15E−04 −2.56 5.9hsa-asg-8683_st1 6.47 0.83 67 1.97E−02 −2.54 5.8 hsa-asg-378_st1 2.710.96 0 3.64E−02 −2.53 5.8 hsa-asg-3009_st1 4.75 0.69 0 8.21E−03 −2.535.8 hsa-asg-6139_st2 2.31 0.60 0 3.97E−03 −2.52 5.7 hsa-asg-2424_st11.84 0.63 0 5.52E−03 −2.52 5.7 hsa-asg-9453_st2 6.94 0.77 33 1.49E−02−2.51 5.7 hsa-asg-13387_st1 4.34 0.93 0 3.37E−02 −2.51 5.7hsa-asg-1273_st2 7.17 1.02 0 4.85E−02 −2.50 5.7 hsa-asg-4903_st2 7.450.96 0 3.80E−02 −2.50 5.7 hsa-asg-11698_st2 7.99 1.00 89 4.49E−02 −2.505.6 hsa-asg-8293_st2 6.47 0.79 0 1.76E−02 −2.49 5.6 hsa-asg-7472_st21.97 0.85 0 2.38E−02 −2.49 5.6 hsa-asg-12289_st1 9.02 0.78 67 1.66E−02−2.47 5.5 hsa-asg-1270_st2 3.63 0.90 0 3.15E−02 −2.47 5.5hsa-asg-1751_st2 7.92 0.95 22 4.00E−02 −2.46 5.5 hsa-asg-6948_st2 7.530.83 100 2.28E−02 −2.45 5.5 hsa-asg-3933_st1 6.92 0.95 33 4.10E−02 −2.455.5 hsa-miR-422a 9.20 0.33 100 1.13E−04 −2.44 5.4 hsa-asg-8541_st1 8.460.67 33 8.70E−03 −2.42 5.4 hsa-asg-3230_st1 6.60 0.85 67 2.65E−02 −2.425.4 hsa-asg-7020_st1 1.99 0.37 0 2.54E−04 −2.42 5.4 hsa-asg-6813_st24.42 0.78 22 1.92E−02 −2.41 5.3 hsa-asg-3742_st1 4.03 0.55 0 3.13E−03−2.41 5.3 hsa-asg-11802_st1 6.99 0.97 0 4.67E−02 −2.40 5.3hsa-asg-9434_st2 6.34 0.68 67 1.04E−02 −2.40 5.3 hsa-asg-6277_st2 6.000.94 67 4.30E−02 −2.38 5.2 hsa-asg-7527_st2 4.21 0.80 0 2.22E−02 −2.375.2 hsa-asg-9382_st2 6.40 0.75 67 1.75E−02 −2.37 5.2 hsa-asg-1619_st23.30 0.78 0 2.13E−02 −2.36 5.1 hsa-asg-960_st1 7.80 0.85 100 3.06E−02−2.35 5.1 hsa-asg-12526_st1 4.04 0.84 0 2.98E−02 −2.35 5.1hsa-asg-8305_st2 6.71 0.81 78 2.52E−02 −2.35 5.1 hsa-cand443_st2 4.590.68 33 1.13E−02 −2.34 5.1 hsa-asg-6068_st2 5.24 0.73 33 1.65E−02 −2.345.1 hsa-asg-12016_st2 2.46 0.84 0 2.96E−02 −2.33 5.0 hsa-asg-2314_st16.14 0.56 44 4.23E−03 −2.32 5.0 hsa-asg-9514_st1 3.43 0.79 0 2.31E−02−2.32 5.0 hsa-asg-11320_st2 4.22 0.31 0 1.13E−04 −2.30 4.9hsa-asg-13878_st2 3.21 0.90 0 4.25E−02 −2.30 4.9 hsa-asg-2195_st1 9.240.90 89 4.23E−02 −2.28 4.8 hsa-asg-3614_st1 4.49 0.59 0 6.63E−03 −2.274.8 hsa-asg-11361_st2 3.39 0.52 0 3.19E−03 −2.27 4.8 hsa-asg-2580_st17.25 0.67 100 1.30E−02 −2.26 4.8 hsa-asg-6637_st1 7.07 0.67 100 1.31E−02−2.25 4.8 hsa-asg-5163_st1 7.45 0.84 0 3.51E−02 −2.25 4.7hsa-asg-8174_st1 3.21 0.53 0 3.86E−03 −2.24 4.7 hsa-asg-9053_st1 5.850.69 33 1.47E−02 −2.24 4.7 hsa-asg-7250_st1 3.77 0.78 0 2.61E−02 −2.244.7 hsa-asg-3545_st1 7.27 0.73 100 2.00E−02 −2.23 4.7 hsa-asg-3672_st15.18 0.80 11 3.01E−02 −2.22 4.7 hsa-asg-3912_st1 2.27 0.74 0 2.09E−02−2.22 4.7 hsa-asg-9830_st2 2.66 0.76 0 2.48E−02 −2.22 4.7hsa-asg-13741_st2 1.94 0.86 0 4.08E−02 −2.22 4.6 hsa-asg-11626_st1 6.900.77 89 2.65E−02 −2.20 4.6 hsa-asg-9009_st1 4.59 0.61 0 8.96E−03 −2.204.6 hsa-asg-7439_st1 5.07 0.90 22 4.90E−02 −2.20 4.6 hsa-asg-10360_st13.29 0.63 0 1.12E−02 −2.19 4.6 hsa-asg-4140_st2 3.58 0.49 0 2.71E−03−2.19 4.6 hsa-cand266_st1 6.38 0.76 78 2.67E−02 −2.18 4.5hsa-asg-11160_st2 3.21 0.79 0 3.05E−02 −2.18 4.5 hsa-asg-1040_st2 5.780.73 22 2.18E−02 −2.18 4.5 hsa-asg-2615_st2 8.27 0.77 100 2.78E−02 −2.184.5 hsa-asg-12687_st1 7.27 0.74 89 2.34E−02 −2.18 4.5 hsa-asg-1794_st11.97 0.57 0 6.58E−03 −2.18 4.5 hsa-asg-466_st1 4.59 0.79 0 3.25E−02−2.16 4.5 hsa-miR-599 3.41 0.78 0 2.99E−02 −2.16 4.5 hsa-asg-11962_st16.76 0.75 78 2.57E−02 −2.16 4.5 hsa-asg-11993_st1 7.75 0.79 0 3.25E−02−2.15 4.4 hsa-cand926_st2 3.81 0.68 0 1.66E−02 −2.15 4.4hsa-asg-3782_st1 6.10 0.57 0 7.24E−03 −2.14 4.4 hsa-asg-8428_st2 1.790.81 0 3.71E−02 −2.13 4.4 hsa-asg-10414_st1 7.28 0.75 89 2.68E−02 −2.134.4 hsa-asg-6282_st1 6.01 0.82 56 3.98E−02 −2.13 4.4 hsa-asg-6063_st28.75 0.61 56 1.08E−02 −2.12 4.4 hsa-asg-12441_st1 7.56 0.79 78 3.38E−02−2.12 4.3 hsa-asg-11923_st1 3.52 0.73 0 2.55E−02 −2.12 4.3hsa-asg-13613_st2 7.71 0.74 100 2.72E−02 −2.11 4.3 hsa-asg-12376_st25.79 0.57 44 7.95E−03 −2.10 4.3 hsa-asg-8565_st1 6.32 0.60 67 1.07E−02−2.09 4.3 hsa-asg-1612_st1 3.17 0.72 0 2.50E−02 −2.07 4.2hsa-asg-1277_st1 8.19 0.69 100 2.25E−02 −2.07 4.2 hsa-asg-3462_st2 8.270.83 100 4.63E−02 −2.06 4.2 hsa-asg-7704_st2 5.39 0.73 33 2.80E−02 −2.064.2 hsa-asg-1596_st2 4.20 0.49 0 3.98E−03 −2.06 4.2 hsa-asg-11165_st17.32 0.77 0 3.41E−02 −2.06 4.2 hsa-asg-9099_st2 4.01 0.82 11 4.47E−02−2.06 4.2 hsa-asg-5413_st2 5.66 0.62 33 1.39E−02 −2.05 4.1hsa-asg-11940_st1 5.55 0.70 22 2.38E−02 −2.04 4.1 hsa-asg-4069_st1 4.120.67 0 2.11E−02 −2.03 4.1 hsa-asg-3646_st1 4.10 0.75 0 3.45E−02 −2.024.1 hsa-asg-8138_st2 5.63 0.79 44 4.22E−02 −2.02 4.1 hsa-cand899_st24.04 0.78 0 3.87E−02 −2.02 4.1 hsa-asg-7159_st2 3.35 0.62 0 1.52E−02−2.02 4.1 hsa-miR112_st1 7.38 0.70 0 2.62E−02 −2.02 4.0 hsa-asg-7654_st26.79 0.72 89 2.90E−02 −2.01 4.0 hsa-asg-7954_st1 7.09 0.74 44 3.29E−02−2.01 4.0 hsa-asg-8329_st1 5.65 0.77 56 3.94E−02 −2.00 4.0 hsa-miR-200a*2.72 0.64 0 1.84E−02 −2.00 4.0 hsa-asg-807_st2 4.86 0.52 11 6.73E−03−2.00 4.0 hsa-asg-12556_st1 7.66 0.76 78 3.70E−02 −1.99 4.0hsa-asg-1576_st1 7.51 0.46 100 3.36E−03 −1.99 4.0 hsa-asg-265_st2 5.970.74 44 3.38E−02 −1.99 4.0 hsa-asg-13471_st1 4.61 0.61 11 1.49E−02 −1.994.0 hsa-asg-10055_st1 1.94 0.66 0 2.14E−02 −1.98 4.0 hsa-cand630_st25.08 0.66 0 2.19E−02 −1.98 3.9 hsa-asg-4207_st1 4.78 0.71 0 2.97E−02−1.98 3.9 hsa-miR-422b 10.7 0.28 100 1.63E−04 −1.98 3.9 hsa-asg-4283_st23.19 0.65 0 2.04E−02 −1.98 3.9 hsa-cand330_st2 4.19 0.80 11 4.65E−02−1.98 3.9 hsa-asg-10478_st2 3.39 0.69 0 2.69E−02 −1.97 3.9hsa-asg-3740_st1 5.10 0.74 0 3.46E−02 −1.97 3.9 hsa-cand852_st2 2.680.51 0 6.79E−03 −1.96 3.9 hsa-asg-3150_st1 6.15 0.62 56 1.75E−02 −1.953.9 hsa-asg-6339_st1 7.43 0.53 100 8.69E−03 −1.93 3.8 hsa-asg-11973_st26.68 0.41 100 2.11E−03 −1.93 3.8 hsa-asg-3746_st1 7.62 0.73 100 3.72E−02−1.93 3.8 hsa-asg-12440_st2 4.66 0.38 0 1.27E−03 −1.93 3.8hsa-asg-3754_st2 4.81 0.57 0 1.21E−02 −1.92 3.8 hsa-asg-8893_st1 4.700.55 0 1.09E−02 −1.92 3.8 hsa-asg-3728_st1 6.29 0.69 56 3.01E−02 −1.923.8 hsa-asg-2624_st2 2.73 0.72 0 3.68E−02 −1.91 3.8 hsa-cand805_st2 8.140.68 100 2.94E−02 −1.91 3.7 hsa-asg-10043_st1 2.60 0.54 0 9.92E−03 −1.903.7 hsa-asg-3406_st1 3.44 0.60 0 1.70E−02 −1.90 3.7 hsa-asg-7671_st16.26 0.67 78 2.87E−02 −1.89 3.7 hsa-asg-1127_st2 3.54 0.54 0 1.09E−02−1.88 3.7 hsa-asg-7228_st1 5.22 0.76 0 4.83E−02 −1.88 3.7hsa-asg-4786_st1 6.85 0.66 89 2.76E−02 −1.86 3.6 hsa-asg-7388_st1 6.280.56 67 1.40E−02 −1.86 3.6 hsa-asg-7579_st2 5.59 0.73 33 4.33E−02 −1.863.6 hsa-miR-517c 2.70 0.70 0 3.69E−02 −1.85 3.6 hsa-asg-12760_st2 3.740.60 0 1.97E−02 −1.85 3.6 hsa-asg-4872_st1 3.56 0.68 0 3.38E−02 −1.833.6 hsa-asg-9273_st2 9.29 0.74 100 4.65E−02 −1.83 3.6 hsa-asg-9511_st26.67 0.47 100 6.12E−03 −1.83 3.6 hsa-cand441_st2 7.31 0.70 56 3.73E−02−1.83 3.6 hsa-asg-9270_st1 2.79 0.74 0 4.63E−02 −1.83 3.6hsa-asg-8193_st2 4.73 0.65 11 2.85E−02 −1.83 3.6 hsa-asg-9412_st2 6.960.71 89 3.97E−02 −1.82 3.5 hsa-asg-12735_st1 6.44 0.63 78 2.56E−02 −1.823.5 hsa-asg-5241_st1 3.17 0.52 0 1.12E−02 −1.81 3.5 hsa-asg-6958_st24.27 0.55 0 1.46E−02 −1.81 3.5 hsa-asg-1922_st2 5.57 0.42 0 3.73E−03−1.81 3.5 hsa-asg-5149_st2 4.86 0.51 0 1.01E−02 −1.80 3.5hsa-asg-5385_st2 5.80 0.40 33 2.84E−03 −1.80 3.5 hsa-asg-4847_st1 6.800.63 78 2.66E−02 −1.79 3.5 hsa-cand6_st2 6.91 0.55 89 1.46E−02 −1.79 3.5hsa-asg-809_st2 5.64 0.71 56 4.50E−02 −1.77 3.4 hsa-asg-748_st1 4.680.66 11 3.55E−02 −1.77 3.4 hsa-asg-1187_st2 5.65 0.64 11 2.99E−02 −1.763.4 hsa-asg-9248_st2 8.14 0.72 100 4.94E−02 −1.76 3.4 hsa-asg-4759_st16.82 0.72 89 4.96E−02 −1.76 3.4 hsa-asg-5289_st2 6.05 0.70 56 4.56E−02−1.75 3.4 hsa-asg-6129_st2 6.89 0.55 89 1.72E−02 −1.75 3.4hsa-asg-13997_st2 2.51 0.65 0 3.42E−02 −1.75 3.4 hsa-asg-8552_st2 10.50.62 100 2.79E−02 −1.74 3.3 hsa-asg-3030_st2 3.71 0.51 0 1.22E−02 −1.743.3 hsa-asg-7278_st1 5.02 0.58 11 2.18E−02 −1.73 3.3 hsa-asg-9165_st16.63 0.69 22 4.34E−02 −1.73 3.3 hsa-asg-6149_st2 6.97 0.68 33 4.22E−02−1.73 3.3 hsa-asg-2637_st1 4.13 0.52 0 1.35E−02 −1.73 3.3hsa-asg-4814_st1 6.24 0.58 78 2.24E−02 −1.72 3.3 hsa-asg-9287_st2 5.620.64 11 3.44E−02 −1.72 3.3 hsa-asg-14051_st2 6.57 0.55 67 1.79E−02 −1.723.3 hsa-asg-12515_st2 3.98 0.45 0 6.72E−03 −1.72 3.3 hsa-asg-1293_st26.04 0.52 56 1.39E−02 −1.71 3.3 hsa-asg-7471_st1 5.65 0.52 56 1.41E−02−1.71 3.3 hsa-asg-8675_st1 5.69 0.55 22 1.92E−02 −1.70 3.3hsa-asg-13999_st1 7.35 0.60 0 2.79E−02 −1.70 3.3 hsa-asg-10772_st1 6.200.47 0 9.20E−03 −1.69 3.2 hsa-asg-12395_st2 5.64 0.64 44 3.52E−02 −1.693.2 hsa-asg-4703_st2 5.24 0.55 22 1.93E−02 −1.69 3.2 hsa-asg-9507_st19.14 0.66 78 4.13E−02 −1.69 3.2 hsa-asg-7336_st1 5.55 0.59 22 2.75E−02−1.69 3.2 hsa-asg-9262_st1 4.46 0.58 0 2.41E−02 −1.68 3.2hsa-asg-12298_st1 4.02 0.57 0 2.42E−02 −1.67 3.2 hsa-asg-5176_st2 2.460.54 0 1.94E−02 −1.66 3.2 hsa-asg-11714_st2 8.25 0.68 11 4.89E−02 −1.663.2 hsa-asg-3345_st1 7.81 0.55 100 2.15E−02 −1.65 3.1 hsa-asg-12625_st13.79 0.67 0 4.77E−02 −1.65 3.1 hsa-asg-12014_st2 8.26 0.54 100 2.06E−02−1.63 3.1 hsa-asg-124_st1 5.02 0.44 0 7.54E−03 −1.63 3.1hsa-asg-4840_st1 4.80 0.61 11 3.54E−02 −1.63 3.1 hsa-asg-10971_st2 6.010.54 78 2.19E−02 −1.62 3.1 hsa-asg-9879_st1 3.19 0.63 0 4.04E−02 −1.623.1 hsa-asg-11795_st2 5.44 0.59 22 3.09E−02 −1.61 3.1 hsa-asg-3952_st18.62 0.62 44 3.87E−02 −1.61 3.1 hsa-miR-576 2.31 0.52 0 1.91E−02 −1.613.1 hsa-asg-14025_st1 6.04 0.54 56 2.18E−02 −1.61 3.1 hsa-asg-13135_st15.11 0.44 22 8.15E−03 −1.61 3.1 hsa-asg-8872_st1 7.17 0.64 89 4.64E−02−1.60 3.0 hsa-asg-11391_st1 2.12 0.63 0 4.23E−02 −1.60 3.0hsa-asg-8163_st1 8.85 0.64 100 4.58E−02 −1.60 3.0 hsa-asg-12777_st2 5.910.48 67 1.32E−02 −1.60 3.0 hsa-asg-10589_st1 2.13 0.61 0 3.82E−02 −1.593.0 hsa-asg-1893_st2 7.61 0.63 100 4.50E−02 −1.58 3.0 hsa-asg-8173_st16.80 0.64 89 4.67E−02 −1.58 3.0 hsa-asg-5265_st1 6.53 0.50 89 1.69E−02−1.58 3.0 hsa-asg-6961_st2 5.19 0.54 0 2.53E−02 −1.57 3.0hsa-asg-12150_st1 7.83 0.61 100 4.12E−02 −1.56 2.9 hsa-asg-6618_st2 5.370.49 22 1.70E−02 −1.56 2.9 hsa-asg-14230_st1 3.82 0.58 0 3.55E−02 −1.552.9 hsa-asg-938_st2 6.51 0.60 78 4.03E−02 −1.54 2.9 hsa-asg-9515_st15.51 0.41 33 7.33E−03 −1.53 2.9 hsa-asg-9500_st1 3.91 0.48 0 1.70E−02−1.53 2.9 hsa-miR223_st2 5.38 0.47 0 1.57E−02 −1.52 2.9 hsa-miR-498 5.490.55 44 3.05E−02 −1.52 2.9 hsa-asg-14217_st1 5.36 0.61 0 4.58E−02 −1.522.9 hsa-asg-1227_st1 4.37 0.60 0 4.52E−02 −1.50 2.8 hsa-asg-8490_st16.02 0.35 11 3.58E−03 −1.49 2.8 hsa-asg-8639_st2 8.92 0.53 78 2.80E−02−1.49 2.8 hsa-asg-6500_st1 4.77 0.59 0 4.46E−02 −1.48 2.8hsa-asg-1715_st2 3.25 0.58 0 4.20E−02 −1.47 2.8 hsa-asg-9334_st2 6.710.50 89 2.42E−02 −1.47 2.8 hsa-asg-8896_st2 4.78 0.54 0 3.43E−02 −1.462.7 hsa-asg-10175_st2 2.19 0.44 0 1.37E−02 −1.45 2.7 hsa-miR-503 6.620.46 78 1.83E−02 −1.45 2.7 hsa-asg-13197_st1 2.87 0.47 0 1.90E−02 −1.442.7 hsa-asg-1168_st1 6.00 0.55 44 3.73E−02 −1.44 2.7 hsa-asg-5047_st14.62 0.58 11 4.56E−02 −1.44 2.7 hsa-asg-773_st1 6.88 0.47 100 1.91E−02−1.44 2.7 hsa-miR-379 6.58 0.37 100 6.02E−03 −1.44 2.7 hsa-asg-2041_st15.59 0.40 33 9.29E−03 −1.44 2.7 hsa-asg-9253_st2 2.46 0.51 0 2.99E−02−1.43 2.7 hsa-asg-8636_st2 6.14 0.54 67 3.60E−02 −1.42 2.7hsa-asg-1903_st2 8.88 0.52 100 3.40E−02 −1.40 2.6 hsa-asg-13334_st2 2.990.41 0 1.18E−02 −1.39 2.6 hsa-miR165_st1 5.22 0.38 11 8.91E−03 −1.39 2.6hsa-asg-2862_st1 6.81 0.37 22 7.66E−03 −1.38 2.6 hsa-asg-9050_st1 7.490.47 100 2.48E−02 −1.37 2.6 hsa-asg-8946_st1 4.45 0.56 0 4.86E−02 −1.372.6 hsa-asg-9209_st2 6.38 0.39 67 1.10E−02 −1.37 2.6 hsa-asg-956_st22.94 0.50 0 3.30E−02 −1.37 2.6 hsa-asg-8188_st1 7.01 0.56 89 4.85E−02−1.36 2.6 hsa-asg-2849_st2 3.38 0.55 33 4.59E−02 −1.36 2.6hsa-asg-13350_st2 8.61 0.41 44 1.62E−02 −1.31 2.5 hsa-asg-9687_st1 4.150.47 0 2.90E−02 −1.31 2.5 hsa-asg-6924_st1 6.55 0.48 78 3.22E−02 −1.302.5 hsa-miR-491 5.47 0.33 0 5.59E−03 −1.29 2.5 hsa-asg-6890_st1 4.280.24 0 8.58E−04 −1.29 2.4 hsa-asg-10291_st2 3.87 0.48 0 3.34E−02 −1.292.4 hsa-cand960_st2 4.87 0.47 11 3.24E−02 −1.29 2.4 hsa-asg-961_st1 3.900.50 0 4.07E−02 −1.28 2.4 hsa-asg-3126_st1 3.85 0.48 0 3.47E−02 −1.282.4 hsa-asg-8039_st2 3.58 0.49 0 3.86E−02 −1.27 2.4 hsa-asg-11481_st23.68 0.38 0 1.38E−02 −1.26 2.4 hsa-asg-10065_st1 4.11 0.39 0 1.50E−02−1.25 2.4 hsa-asg-1319_st2 5.73 0.46 11 3.24E−02 −1.25 2.4hsa-asg-7170_st2 3.91 0.49 0 4.18E−02 −1.24 2.4 hsa-asg-1689_st2 5.410.36 11 1.12E−02 −1.24 2.4 hsa-asg-9491_st1 4.55 0.45 0 3.06E−02 −1.242.4 hsa-asg-381_st1 3.30 0.40 0 1.82E−02 −1.24 2.4 hsa-asg-8253_st2 9.700.50 100 4.74E−02 −1.23 2.3 hsa-cand753_st1 5.36 0.40 33 2.05E−02 −1.222.3 hsa-asg-2542_st1 4.87 0.50 0 4.89E−02 −1.22 2.3 hsa-asg-125_st1 8.080.43 0 2.80E−02 −1.22 2.3 hsa-asg-3722_st1 8.92 0.37 100 1.48E−02 −1.192.3 hsa-asg-4208_st1 3.66 0.37 0 1.60E−02 −1.19 2.3 hsa-asg-9411_st15.09 0.34 11 1.10E−02 −1.18 2.3 hsa-asg-11900_st1 5.69 0.45 56 3.57E−02−1.18 2.3 hsa-asg-3224_st1 4.18 0.45 0 3.81E−02 −1.17 2.2hsa-cand659_st1 7.78 0.25 100 2.50E−03 −1.16 2.2 hsa-asg-12874_st1 4.420.33 0 1.18E−02 −1.14 2.2 hsa-asg-12216_st1 3.69 0.27 0 4.10E−03 −1.122.2 hsa-asg-917_st2 5.13 0.43 33 3.79E−02 −1.11 2.2 hsa-asg-6845_st22.70 0.33 0 1.35E−02 −1.09 2.1 hsa-asg-1896_st2 3.13 0.44 0 4.60E−02−1.08 2.1 hsa-cand764_st1 5.84 0.42 44 4.05E−02 −1.08 2.1hsa-asg-4118_st1 6.78 0.37 89 2.79E−02 −1.06 2.1 hsa-asg-4836_st2 5.580.32 44 1.50E−02 −1.04 2.1 hsa-miR203_st1 6.10 0.40 33 4.47E−02 −1.012.0 hsa-miR-496 2.11 0.38 0 3.58E−02 −1.01 2.0 SD, standard deviation;%, percentage of samples expressing miRNA above background levels;Log2Diff, difference in VSN-transformed expression bewteen THY and LNnegsamples.

Example 28 miRNAs Differentally Expressed Between Normal Kidney Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous kidney cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal kidneytissue than in cancer-negative lymph nodes.

Total RNA, purified from one normal human kidney sample (FirstChoice®Human Kidney Total RNA, cat. no. AM7976; Ambion, Austin, Tex., USA) andfrom nine normal human lymph node samples (FirstChoice® Human Lymph NodeTotal RNA, cat. no. AM7894; Ambion, Austin, Tex., USA) was purchasedfrom Ambion. miRNA purification and labeling and microarray expressionanalyses were performed as described above in Example 2.

The inventors identified 173 miRNAs (75 verified, 98 predicted) withsignificant differential expression between the normal kidney and normallymph node samples (log 2Diff kidney vs LN neg≧1 and p-value<0.05)(Table 27). Among the verified miRNAs, hsa-miR-429 was expressed at alevel more than 200-fold higher in the kidney tissue sample;hsa-miR-200a, -187, and -204 were expressed between 50- and 100-foldhigher in the kidney sample; hsa-miR-200b, -489, -184 and -215 wereexpressed between 20- and 50-fold higher in kidney; 16 miRNAs wereexpressed between 10- and 20-fold higher in kidney; and 24 miRNAs wereexpressed between 5- and 10-fold higher in the kidney sample than innormal lymph nodes. Among the predicted miRNAs, hsa-asg-4441_st1expression in the kidney sample was over 50-fold higher than in thenormal lymph node samples; hsa-miR-14182 st1, -14153_st2, -7637_st2 and-4915_st1 were expressed between 20- and 50-fold higher in the kidneysample; 11 miRNAs were expressed between 10- and 20-fold higher inkidney; and 28 miRNAs were expressed between 5- and 10-fold higher inthe kideny sample than in the normal lymph nodes.

TABLE 27 MicroRNAs Differentially Expressed Between Normal Kidney (KID)and Cancer-Negative Lymph Node Samples (LNneg) from Patients. ProbeLNneg MicroRNA ID KID 1 2 3 4 5 6 hsa-miR-429 hsa-miR-429_st1 10.92 3.421.54 3.66 2.10 2.33 2.65 hsa-miR-200a hsa-miR-200a_st2 12.12 6.26 5.026.49 5.71 5.25 4.54 hsa-miR-187 hsa-miR-187_st1 9.50 4.74 2.75 2.74 3.753.18 3.32 hsa-asg-4441_st1 hsa-asg-4441_st1 9.93 3.53 3.42 4.42 4.564.07 3.32 hsa-miR-204 hsa-miR-204_st2 11.92 7.17 5.77 5.98 8.20 6.735.40 hsa-miR-200b hsa-miR-200b_st1 13.30 7.61 7.75 8.91 6.69 6.79 6.82hsa-asg-14182_st1 hsa-asg-14182_st1 7.49 2.41 0.97 1.89 1.79 2.57 1.76hsa-asg-14153_st2 hsa-asg-14153_st2 8.09 3.05 2.23 3.04 2.98 2.33 2.65hsa-miR-489 hsa-miR-489_st2 11.04 7.34 6.75 6.01 7.47 6.33 5.45hsa-miR-184 hsa-miR-184_st1 8.76 5.50 4.50 4.11 4.28 3.89 5.11hsa-miR-215 hsa-miR-215_st1 10.15 6.95 5.77 5.71 6.66 5.51 4.79hsa-asg-7637_st2 hsa-asg-7637_st2 8.23 3.64 2.50 4.46 3.44 3.89 3.97hsa-asg-4915_st1 hsa-asg-4915_st1 7.33 2.41 3.78 2.15 2.57 3.59 2.87hsa-miR-493-5p hsa-miR-493-5p_st1 8.91 4.35 3.78 6.89 6.18 6.30 3.63hsa-asg-11715_st2 hsa-asg-11715_st2 6.14 1.83 2.32 2.15 1.48 2.07 1.76hsa-miR-424 hsa-miR-424_st1 9.91 5.79 6.11 5.53 5.91 5.75 3.43hsa-asg-1088_st1 hsa-asg-1088_st1 6.11 1.83 2.13 1.63 1.59 2.07 0.87hsa-miR-203 hsa-miR-203_st2 10.90 7.32 5.84 7.22 8.24 6.42 5.36hsa-miR-200a* hsa-miR-200a-_st2 6.80 3.05 2.83 2.15 3.89 2.57 1.76hsa-miR-194 hsa-miR-194_st2 13.00 9.82 9.32 8.92 9.34 8.92 8.29hsa-miR-200c hsa-miR-200c_st1 13.10 9.59 8.79 10.3 8.17 8.12 8.08hsa-asg-1260_st1 hsa-asg-1260_st1 6.64 2.41 2.83 2.40 3.19 2.57 3.08hsa-miR-124a hsa-cand79_st1 7.54 3.53 3.78 3.78 4.44 3.64 2.14hsa-miR-124a hsa-miR-124a_st1 7.55 4.29 4.18 3.78 4.80 3.81 3.32hsa-asg-924_st1 hsa-asg-924_st1 7.39 4.64 4.25 3.38 4.44 2.65 2.73hsa-asg-11443_st1 hsa-asg-11443_st1 7.03 4.15 3.58 2.84 2.66 3.50 2.65hsa-miR-450 hsa-asg-14228_st2 6.62 3.53 2.13 2.40 2.98 2.49 3.08hsa-miR-450 hsa-miR-450_st2 7.42 4.22 2.67 2.84 3.89 3.59 3.97 hsa-miR-9hsa-miR-9_st2 7.09 3.42 3.48 2.84 3.25 3.59 2.14 hsa-miR-9hsa-cand90_st2 6.28 3.53 3.11 2.63 2.98 2.79 2.14 hsa-asg-12227_st1hsa-asg-12227_st1 5.65 1.63 0.80 2.15 1.18 1.89 1.95 hsa-miR-605hsa-miR-605_st1 6.12 2.58 2.13 3.04 0.73 1.71 2.14 hsa-miR-542-5phsa-miR-542-5p_st2 7.39 3.91 2.83 3.66 3.60 3.81 2.80 hsa-asg-12799_st1hsa-asg-12799_st1 7.77 3.53 5.30 3.30 2.90 4.20 5.40 hsa-miR144_st2hsa-miR144_st2 6.92 4.59 4.38 2.63 3.38 2.65 2.65 hsa-asg-5638_st2hsa-asg-5638_st2 6.05 4.00 2.50 2.15 2.57 1.62 2.57 hsa-miR-192hsa-miR-192_st2 13.14 10.8 9.82 10.1 10.0 9.55 8.66 hsa-miR-154hsa-miR-154_st2 6.91 3.73 2.50 4.30 4.35 4.88 3.08 hsa-asg-14208_st1hsa-asg-14208_st1 7.03 5.05 3.48 4.46 4.14 3.35 1.21 hsa-miR-411hsa-miR-411_st1 7.20 4.84 3.68 4.54 5.22 5.52 2.94 hsa-miR-141hsa-miR-141_st1 7.52 5.05 3.78 4.93 4.41 4.36 2.80 hsa-miR-582hsa-miR-582_st1 7.84 5.84 2.13 4.54 5.95 4.86 4.05 hsa-asg-4978_st2hsa-asg-4978_st2 5.83 1.63 2.83 2.84 1.69 1.98 1.95 hsa-miR-96hsa-miR-96_st1 6.55 4.97 3.36 3.04 2.98 4.07 2.80 hsa-asg-4781_st2hsa-asg-4781_st2 7.15 4.79 4.11 4.46 1.69 2.16 3.43 hsa-asg-5119_st1hsa-asg-5119_st1 5.79 2.41 2.67 1.89 1.90 1.98 2.49 hsa-miR-135bhsa-miR-135b_st2 5.85 1.43 2.67 2.40 2.82 2.33 3.08 hsa-miR-135bhsa-cand78_st1 5.95 3.42 2.50 3.66 4.14 3.68 2.80 hsa-asg-4685_st1hsa-asg-4685_st1 7.05 3.31 4.50 3.52 3.25 4.36 3.32 hsa-miR-653hsa-miR-653_st1 5.30 2.03 2.83 1.12 3.50 2.16 2.14 hsa-miR-487bhsa-miR-487b_st2 8.71 6.05 5.79 6.97 6.90 6.22 5.34 hsa-asg-4256_st2hsa-asg-4256_st2 6.54 4.00 4.03 1.89 2.82 5.09 3.81 hsa-asg-2537_st1hsa-asg-2537_st1 6.89 3.42 3.86 3.66 4.41 3.64 3.72 hsa-asg-5721_st2hsa-asg-5721_st2 5.59 2.22 3.36 2.15 3.12 1.98 2.32 hsa-asg-13705_st1hsa-asg-13705_st1 5.36 1.63 0.97 3.78 2.30 2.65 2.32 hsa-asg-5827_st2hsa-asg-5827_st2 8.30 5.61 4.71 5.71 5.52 5.65 5.15 hsa-asg-5227_st1hsa-asg-5227_st1 5.61 1.43 2.97 2.15 2.10 1.89 3.63 hsa-miR-539hsa-miR-539_st1 5.57 2.03 3.11 2.63 3.38 2.65 2.94 hsa-asg-11160_st2hsa-asg-11160_st2 6.26 2.75 3.11 3.38 3.05 2.86 2.65 hsa-miR-501hsa-miR-501_st1 7.42 5.24 4.81 2.84 5.09 4.04 4.60 hsa-asg-10247_st1hsa-asg-10247_st1 5.49 2.75 2.13 4.01 1.69 2.16 2.32 hsa-asg-8174_st1hsa-asg-8174_st1 6.15 3.31 4.03 2.84 2.98 2.86 3.20 hsa-asg-10131_st2hsa-asg-10131_st2 6.15 3.31 3.11 3.52 2.48 2.49 3.20 hsa-miR-189hsa-miR-189_st1 8.19 6.07 5.74 4.93 7.23 6.15 4.49 hsa-asg-1296_st1hsa-asg-1296_st1 6.10 3.05 2.50 4.81 2.39 2.49 2.73 hsa-cand283_st1hsa-cand283_st1 7.99 5.44 6.09 4.68 5.55 4.71 5.57 hsa-asg-5190_st1hsa-asg-5190_st1 5.41 3.73 2.50 2.15 2.66 2.72 3.32 hsa-miR-542-3phsa-miR-542-3p_st1 8.33 5.09 5.25 6.01 6.29 5.71 5.62 hsa-miR-485-3phsa-miR23_st2 6.02 4.29 2.50 4.11 3.25 2.41 3.20 hsa-miR-485-3phsa-miR-485-3p_st2 5.49 3.64 2.97 4.30 4.41 4.24 3.08 hsa-asg-9696_st1hsa-asg-9696_st1 9.23 7.42 6.31 6.68 7.04 6.23 6.22 hsa-asg-5617_st1hsa-asg-5617_st1 5.52 3.73 3.24 3.90 3.60 3.40 2.65 hsa-miR-138hsa-miR-138_st1 8.36 6.36 5.68 6.82 6.41 5.91 4.57 hsa-miR-376ahsa-asg-10049_st1 7.56 5.01 4.85 6.09 5.55 5.31 4.05 hsa-miR-376ahsa-miR-376a_st2 6.97 5.13 4.03 5.49 5.66 4.82 3.97 hsa-miR-376ahsa-asg-14234_st1 6.14 3.31 4.25 3.38 3.98 4.82 3.63 hsa-miR-381hsa-miR-381_st2 5.77 2.75 2.32 4.21 3.38 4.17 2.94 hsa-miR-19ahsa-miR-19a_st1 5.47 4.59 3.36 2.84 3.12 3.59 2.49 hsa-miR-410hsa-miR-410_st1 5.15 2.41 2.32 1.37 3.71 2.49 2.80 hsa-miR-503hsa-miR-503_st1 9.23 6.88 7.17 7.38 6.60 6.08 6.45 hsa-miR-503hsa-miR141_st1 9.18 7.10 7.28 7.30 6.52 6.55 6.62 hsa-asg-6377_st2hsa-asg-6377_st2 5.27 2.22 2.83 3.66 1.90 1.71 3.43 hsa-asg-12907_st2hsa-asg-12907_st2 5.27 3.73 2.83 3.52 2.48 2.79 1.95 hsa-miR-499hsa-miR12_st1 7.45 5.44 4.76 5.37 6.66 4.07 4.38 hsa-miR-499hsa-miR-499_st1 7.28 5.31 4.61 4.75 6.57 4.49 4.12 hsa-asg-14146_st1hsa-asg-14146_st1 8.38 6.29 5.72 6.24 6.49 5.92 4.79 hsa-miR-218hsa-miR-218_st2 11.33 9.61 9.22 8.83 9.68 9.13 8.59 hsa-cand206_st1hsa-cand206_st1 5.21 2.22 1.94 3.78 3.60 2.65 2.14 hsa-miR-660hsa-miR-660_st1 11.06 9.28 8.94 7.94 9.15 8.60 8.38 hsa-miR-362hsa-miR-362_st1 9.82 7.42 7.66 7.94 7.60 7.44 7.08 hsa-miR-9*hsa-miR-9-_st2 8.06 6.19 6.05 5.57 6.10 5.37 5.81 hsa-miR-577hsa-miR-577_st1 5.27 4.59 2.83 3.21 2.98 2.79 1.39 hsa-asg-9681_st1hsa-asg-9681_st1 6.61 4.84 4.25 4.99 4.75 3.68 2.80 hsa-miR-369-5phsa-asg-1521_st1 5.84 3.73 3.36 4.01 2.20 3.68 3.68 hsa-miR-369-5phsa-miR-369-5p_st2 5.87 3.53 2.67 4.46 4.35 4.33 2.49 hsa-asg-14230_st1hsa-asg-14230_st1 6.29 4.29 3.68 4.38 4.56 3.29 3.43 hsa-asg-13908_st2hsa-asg-13908_st2 5.08 2.03 2.50 3.66 2.48 1.44 2.80 hsa-asg-11361_st2hsa-asg-11361_st2 5.81 2.75 3.48 3.38 3.25 3.59 3.32 hsa-miR-500hsa-miR-500_st1 10.81 8.74 8.38 8.78 8.81 8.16 7.79 hsa-asg-9574_st2hsa-asg-9574_st2 8.22 6.61 6.74 4.46 5.50 5.60 6.11 hsa-miR-592hsa-miR-592_st1 5.30 4.29 2.50 2.84 3.12 1.80 1.76 hsa-miR102_st2hsa-miR102_st2 10.70 8.89 8.26 8.71 8.69 8.11 7.77 hsa-miR-30e-5phsa-miR-30e-5p_st2 11.89 10.3 9.59 9.72 10.5 9.78 9.18 hsa-asg-1268_st1hsa-asg-1268_st1 5.98 4.74 3.24 4.01 4.14 3.45 3.72 hsa-asg-128_st2hsa-asg-128_st2 6.86 4.84 4.71 4.54 5.11 4.52 4.12 hsa-asg-13997_st2hsa-asg-13997_st2 4.74 3.05 3.24 2.15 2.98 2.16 2.32 hsa-asg-548_st1hsa-asg-548_st1 5.27 3.05 1.94 3.52 3.50 2.79 1.95 hsa-asg-12552_st1hsa-asg-12552_st1 6.45 4.07 3.78 3.52 4.44 3.40 4.15 hsa-asg-11612_st1hsa-asg-11612_st1 7.28 5.17 4.96 4.71 5.67 4.54 4.65 hsa-asg-11338_st2hsa-asg-11338_st2 6.54 4.07 5.59 3.52 4.28 4.36 4.92 hsa-asg-6642_st2hsa-asg-6642_st2 5.76 4.00 3.78 4.38 3.85 3.68 3.48 hsa-asg-1221_st2hsa-asg-1221_st2 5.64 4.00 3.24 4.38 4.21 3.73 3.08 hsa-asg-7471_st1hsa-asg-7471_st1 7.75 5.58 5.67 5.68 6.74 5.67 5.25 hsa-miR-154*hsa-miR-154-_st2 6.39 5.81 3.48 4.21 4.98 4.59 3.89 hsa-asg-1596_st2hsa-asg-1596_st2 6.27 4.41 5.06 4.01 3.19 4.36 4.12 hsa-miR-183hsa-miR-183_st1 9.03 7.65 6.71 6.71 7.93 7.54 5.78 hsa-miR-148bhsa-miR-148b_st2 11.21 10.0 8.74 9.45 10.0 9.60 8.22 hsa-asg-9526_st2hsa-asg-9526_st2 7.96 5.37 6.31 4.68 5.00 6.41 6.22 hsa-miR-455hsa-miR-455_st1 7.22 5.81 4.11 5.81 6.00 5.67 4.70 hsa-asg-6381_st1hsa-asg-6381_st1 5.83 4.22 4.85 2.40 3.98 3.64 4.25 hsa-asg-9687_st1hsa-asg-9687_st1 6.11 4.74 4.76 3.90 4.65 3.85 4.19 hsa-asg-8731_st2hsa-asg-8731_st2 6.40 3.91 4.85 4.30 4.02 4.80 4.25 hsa-asg-5986_st1hsa-asg-5986_st1 9.11 6.99 7.15 6.21 7.52 6.14 6.77 hsa-miR-363hsa-miR-363_st2 11.72 10.5 9.97 9.91 10.2 9.02 9.35 hsa-asg-4608_st1hsa-asg-4608_st1 6.31 3.42 4.85 4.93 4.38 4.00 4.57 hsa-miR-487ahsa-asg-6915_st1 5.57 4.07 3.58 3.90 3.38 3.85 3.72 hsa-asg-4637_st1hsa-asg-4637_st1 8.26 7.08 6.44 6.38 6.52 6.47 6.47 hsa-miR-494hsa-miR-494_st1 8.52 6.39 7.25 7.23 6.95 6.60 7.61 hsa-miR-494hsa-miR61_st2 7.84 6.81 6.50 6.86 6.42 6.35 7.05 hsa-miR-324-5phsa-miR-324-5p_st1 9.75 7.91 8.08 8.34 8.26 7.77 7.37 hsa-asg-12666_st2hsa-asg-12666_st2 6.62 6.07 4.61 4.87 4.89 4.62 4.54 hsa-miR-19bhsa-miR-19b_st2 9.26 8.37 8.28 7.32 8.02 8.01 6.45 hsa-asg-13279_st1hsa-asg-13279_st1 9.03 7.57 7.42 7.55 8.17 7.21 6.54 hsa-asg-10775_st2hsa-asg-10775_st2 6.54 5.09 4.71 4.81 5.05 4.33 4.62 hsa-asg-10626_st2hsa-asg-10626_st2 6.62 5.13 5.14 4.61 5.36 4.96 4.25 hsa-asg-12825_st1hsa-asg-12825_st1 5.30 3.05 3.11 2.40 4.48 3.73 4.49 hsa-asg-3998_st1hsa-asg-3998_st1 10.48 8.62 8.53 8.70 9.41 8.74 8.32 hsa-miR-510hsa-miR-510_st2 6.54 5.27 5.02 4.75 4.65 4.71 5.04 hsa-asg-9053_st1hsa-asg-9053_st1 7.55 5.86 6.50 4.78 6.21 6.07 6.86 hsa-miR-518c*hsa-miR-518c-_st2 5.66 3.83 5.14 4.38 3.12 3.35 4.38 hsa-asg-3503_st2hsa-asg-3503_st2 5.30 3.31 3.68 3.38 3.12 3.97 3.32 hsa-miR-130ahsa-miR-130a_st1 11.72 10.3 9.91 9.72 10.9 10.7 9.16 hsa-miR-130ahsa-cand144_st1 11.72 10.4 9.80 9.72 10.9 10.7 9.20 hsa-asg-10336_st1hsa-asg-10336_st1 5.74 4.74 3.95 3.52 4.32 4.59 3.77 hsa-asg-2237_st1hsa-asg-2237_st1 5.99 4.93 4.66 3.90 4.82 4.24 3.89 hsa-asg-3046_st2hsa-asg-3046_st2 7.22 5.13 6.50 4.93 5.84 5.09 6.22 hsa-miR-329hsa-asg-13204_st2 5.18 4.07 2.83 4.21 3.38 4.20 3.43 hsa-asg-13298_st1hsa-asg-13298_st1 7.32 5.17 6.20 4.87 5.96 5.40 6.18 hsa-asg-3289_st1hsa-asg-3289_st1 5.08 3.64 4.25 3.21 3.89 3.06 3.81 hsa-asg-12922_st1hsa-asg-12922_st1 6.01 4.47 4.55 3.21 4.56 4.52 4.88 hsa-asg-4416_st1hsa-asg-4416_st1 5.15 3.64 4.03 4.11 4.06 3.50 3.72 hsa-asg-10830_st2hsa-asg-10830_st2 5.44 4.35 3.95 3.66 3.89 3.89 4.43 hsa-asg-1301_st1hsa-asg-1301_st1 5.33 4.47 3.68 3.90 3.71 3.77 3.63 hsa-asg-10298_st1hsa-asg-10298_st1 5.61 4.53 3.78 4.38 4.21 3.93 3.97 hsa-miR-422ahsa-miR-422a_st1 10.74 9.09 8.97 9.17 9.80 8.95 8.80 hsa-asg-14051_st2hsa-asg-14051_st2 8.08 6.09 7.27 5.94 5.91 6.88 7.07 hsa-miR257_st1hsa-miR257_st1 10.31 9.61 9.05 9.23 9.55 8.84 8.49 hsa-miR-106bhsa-miR-106b_st2 10.95 9.90 9.59 10.0 10.0 9.40 8.12 hsa-miR-210hsa-miR-210_st1 8.07 6.50 5.60 6.85 7.08 7.08 6.66 hsa-asg-7278_st1hsa-asg-7278_st1 6.50 5.20 5.73 4.34 4.92 5.28 5.54 hsa-asg-8512_st2hsa-asg-8512_st2 5.47 4.41 4.44 3.21 4.21 4.04 3.63 hsa-miR-422bhsa-miR-422b_st2 12.15 10.6 10.5 10.7 11.3 10.6 10.3 hsa-miR-181a*hsa-miR-213_st1 7.24 6.29 6.17 5.68 5.71 5.42 5.37 hsa-miR-379hsa-miR-379_st1 8.00 6.51 6.37 7.12 6.96 7.01 6.54 hsa-miR-30a-5phsa-miR-30a-5p_st2 13.26 12.2 12.0 11.9 12.5 12.0 11.6 hsa-miR-370hsa-miR-370_st1 6.20 4.64 5.23 4.71 5.05 4.69 5.34 hsa-asg-8160_st2hsa-asg-8160_st2 8.43 6.86 7.61 6.38 6.98 7.48 7.48 hsa-asg-13135_st1hsa-asg-13135_st1 6.42 5.61 5.61 5.24 4.89 4.76 4.70 hsa-miR-151hsa-miR-151_st2 10.65 10.1 9.71 9.15 9.80 9.35 9.42 hsa-miR-98hsa-miR-98_st1 12.28 11.5 11.0 11.1 11.5 11.0 10.8 hsa-asg-4693_st1hsa-asg-4693_st1 6.43 5.05 5.74 4.21 5.38 5.00 5.21 hsa-asg-9629_st2hsa-asg-9629_st2 6.05 4.93 5.35 4.21 4.51 5.11 4.96 hsa-asg-2592_st1hsa-asg-2592_st1 7.76 6.50 7.12 5.55 6.41 6.78 6.72 hsa-asg-225_st1hsa-asg-225_st1 6.83 5.64 6.52 5.12 5.75 5.48 5.79 hsa-asg-2402_st1hsa-asg-2402_st1 5.15 4.41 3.95 3.38 3.80 3.29 4.05 hsa-miR-421hsa-asg-5033_st2 9.40 8.21 8.07 8.49 8.61 7.77 7.98 hsa-miR-421hsa-miR-421-3p_st1 10.08 9.49 9.15 8.99 9.42 8.68 8.78 hsa-asg-7131_st1hsa-asg-7131_st1 8.11 6.24 7.21 6.71 7.29 6.60 7.16 hsa-miR-30dhsa-miR-30d_st1 13.49 12.7 12.1 12.5 12.9 12.4 11.7 hsa-miR-454-3phsa-asg-11541_st2 9.22 8.62 8.26 8.54 8.45 7.84 7.87 hsa-asg-12697_st1hsa-asg-12697_st1 5.05 3.31 4.31 4.01 3.60 3.85 4.38 hsa-asg-3156_st1hsa-asg-3156_st1 6.03 4.93 5.53 4.61 4.73 4.96 4.49 hsa-asg-10007_st1hsa-asg-10007_st1 8.47 7.15 7.80 6.62 7.01 7.45 7.60 hsa-asg-14233_st1hsa-asg-14233_st1 10.35 9.44 9.21 9.23 10.1 9.41 8.84 hsa-asg-5385_st2hsa-asg-5385_st2 6.88 5.79 6.49 5.59 5.50 5.73 6.35 hsa-asg-7713_st2hsa-asg-7713_st2 5.88 4.53 4.90 4.30 4.73 5.11 4.70 LNneg MicroRNA 7 8 9M SD % ttest log2Diff Fold hsa-miR-429 2.31 2.41 5.36 2.86 1.14 01.51E−04 8.06 266. hsa-miR-200a 4.05 4.75 7.76 5.54 1.15 67 6.21E−046.58 95.5 hsa-miR-187 3.03 3.35 3.14 3.33 0.61 0 1.21E−05 6.16 71.7hsa-asg-4441_st1 3.98 5.55 4.54 4.16 0.71 0 5.56E−05 5.77 54.6hsa-miR-204 5.17 5.40 5.76 6.18 1.00 44 6.07E−04 5.74 53.5 hsa-miR-200b6.50 7.64 10.41 7.68 1.27 100 2.97E−03 5.63 49.4 hsa-asg-14182_st1 2.771.85 0.73 1.86 0.68 0 5.06E−05 5.63 49.4 hsa-asg-14153_st2 1.17 2.744.37 2.73 0.85 0 3.32E−04 5.37 41.2 hsa-miR-489 5.71 5.40 5.20 6.18 0.8456 6.08E−04 4.85 28.9 hsa-miR-184 1.60 3.80 4.13 4.10 1.09 11 3.69E−034.66 25.3 hsa-miR-215 5.49 5.09 5.20 5.69 0.71 33 3.29E−04 4.47 22.1hsa-asg-7637_st2 3.27 3.61 5.24 3.78 0.77 0 5.77E−04 4.45 21.9hsa-asg-4915_st1 2.77 2.05 4.44 2.96 0.81 0 9.06E−04 4.37 20.7hsa-miR-493-5p 4.36 1.85 4.58 4.66 1.58 33 3.37E−02 4.25 19.0hsa-asg-11715_st2 0.63 1.46 3.36 1.90 0.74 0 6.26E−04 4.24 18.9hsa-miR-424 5.80 5.70 7.21 5.69 0.98 89 3.51E−03 4.22 18.7hsa-asg-1088_st1 3.27 1.46 2.51 1.93 0.68 0 4.06E−04 4.19 18.2hsa-miR-203 5.17 6.05 9.34 6.77 1.38 78 2.22E−02 4.13 17.5 hsa-miR-200a*2.14 3.17 2.89 2.72 0.64 0 3.20E−04 4.08 16.9 hsa-miR-194 8.54 8.20 9.038.93 0.53 100 8.38E−05 4.07 16.8 hsa-miR-200c 7.96 8.87 11.51 9.05 1.21100 1.33E−02 4.05 16.6 hsa-asg-1260_st1 2.90 2.41 1.60 2.60 0.48 04.42E−05 4.04 16.4 hsa-miR-124a 2.31 3.40 4.82 3.54 0.87 0 2.42E−03 4.0116.1 hsa-miR-124a 2.14 3.61 4.37 3.81 0.77 0 1.75E−03 3.74 13.3hsa-asg-924_st1 2.14 2.74 3.50 3.38 0.89 0 2.83E−03 4.00 16.0hsa-asg-11443_st1 3.38 2.74 2.29 3.09 0.59 0 2.31E−04 3.94 15.3hsa-miR-450 2.90 2.89 2.71 2.79 0.42 0 2.28E−05 3.83 14.2 hsa-miR-4504.18 4.44 4.04 3.76 0.62 0 5.02E−04 3.66 12.6 hsa-miR-9 3.48 3.29 4.133.29 0.55 0 1.76E−04 3.79 13.9 hsa-miR-9 2.62 1.66 3.62 2.79 0.63 07.40E−04 3.49 11.2 hsa-asg-12227_st1 2.77 2.58 2.71 1.96 0.68 0 8.81E−043.68 12.9 hsa-miR-605 2.90 3.03 4.44 2.52 1.03 0 1.07E−02 3.60 12.1hsa-miR-542-5p 3.83 4.66 5.16 3.81 0.76 0 2.04E−03 3.58 12.0hsa-asg-12799_st1 3.91 4.29 4.84 4.19 0.87 0 4.61E−03 3.58 12.0hsa-miR144_st2 2.77 3.17 4.21 3.38 0.81 0 3.11E−03 3.54 11.7hsa-asg-5638_st2 1.25 2.74 3.22 2.51 0.81 0 3.31E−03 3.53 11.6hsa-miR-192 9.31 8.79 9.63 9.64 0.67 100 1.13E−03 3.50 11.3 hsa-miR-1543.15 1.28 3.94 3.47 1.10 0 1.83E−02 3.44 10.8 hsa-asg-14208_st1 3.034.04 3.94 3.63 1.09 0 1.85E−02 3.40 10.6 hsa-miR-411 3.38 2.23 2.07 3.831.27 11 3.62E−02 3.37 10.4 hsa-miR-141 3.83 3.80 4.51 4.16 0.69 01.76E−03 3.36 10.3 hsa-miR-582 4.05 4.32 4.64 4.49 1.12 22 2.21E−02 3.3510.2 hsa-asg-4978_st2 2.77 2.58 4.13 2.49 0.79 0 3.76E−03 3.34 10.1hsa-miR-96 3.03 1.85 3.06 3.24 0.87 11 6.76E−03 3.31 9.9hsa-asg-4781_st2 5.36 5.46 3.22 3.85 1.34 11 4.72E−02 3.30 9.9hsa-asg-5119_st1 2.90 2.58 3.62 2.49 0.55 0 4.88E−04 3.30 9.8hsa-miR-135b 3.27 2.23 2.89 2.57 0.55 0 4.80E−04 3.28 9.7 hsa-miR-135b3.57 1.66 3.22 3.18 0.75 0 8.32E−03 2.76 6.8 hsa-asg-4685_st1 3.03 4.324.76 3.82 0.65 0 1.55E−03 3.23 9.4 hsa-miR-653 2.47 1.66 1.14 2.11 0.770 4.29E−03 3.19 9.1 hsa-miR-487b 4.88 3.71 4.37 5.58 1.11 67 2.81E−023.13 8.8 hsa-asg-4256_st2 3.98 1.28 3.84 3.42 1.19 0 3.82E−02 3.12 8.7hsa-asg-2537_st1 3.38 3.71 4.13 3.77 0.33 0 1.84E−05 3.12 8.7hsa-asg-5721_st2 1.78 2.89 2.51 2.48 0.54 0 5.74E−04 3.11 8.6hsa-asg-13705_st1 0.37 3.17 3.22 2.27 1.11 0 2.95E−02 3.09 8.5hsa-asg-5827_st2 4.76 5.16 4.70 5.22 0.42 22 1.18E−04 3.08 8.5hsa-asg-5227_st1 2.90 3.40 2.51 2.56 0.73 0 4.08E−03 3.06 8.3hsa-miR-539 1.96 2.58 1.36 2.52 0.63 0 1.81E−03 3.05 8.3hsa-asg-11160_st2 2.47 3.51 5.11 3.21 0.79 0 6.37E−03 3.05 8.3hsa-miR-501 4.47 4.25 4.37 4.41 0.70 0 3.64E−03 3.01 8.0hsa-asg-10247_st1 0.37 3.17 3.94 2.50 1.14 0 3.73E−02 2.99 7.9hsa-asg-8174_st1 2.77 2.74 4.13 3.21 0.53 0 7.47E−04 2.95 7.7hsa-asg-10131_st2 2.62 3.17 5.07 3.22 0.79 0 7.67E−03 2.93 7.6hsa-miR-189 4.92 3.51 4.92 5.33 1.09 44 3.74E−02 2.86 7.3hsa-asg-1296_st1 3.15 3.88 4.13 3.24 0.85 0 1.29E−02 2.86 7.3hsa-cand283_st1 5.24 4.55 4.51 5.15 0.56 33 1.31E−03 2.84 7.2hsa-asg-5190_st1 1.78 2.23 2.51 2.62 0.60 0 2.17E−03 2.79 6.9hsa-miR-542-3p 5.38 5.88 4.70 5.55 0.49 78 6.91E−04 2.78 6.9hsa-miR-485-3p 3.57 2.74 3.06 3.24 0.66 0 3.96E−03 2.78 6.9hsa-miR-485-3p 3.66 3.51 3.84 3.74 0.51 0 1.20E−02 1.75 3.4hsa-asg-9696_st1 6.61 6.53 5.07 6.46 0.65 89 3.81E−03 2.77 6.8hsa-asg-5617_st1 1.42 1.46 1.60 2.78 1.03 0 3.53E−02 2.74 6.7hsa-miR-138 4.92 4.87 5.09 5.62 0.80 33 1.18E−02 2.73 6.7 hsa-miR-376a3.83 4.12 4.64 4.83 0.75 11 8.79E−03 2.73 6.6 hsa-miR-376a 2.47 3.803.73 4.34 1.02 0 4.04E−02 2.63 6.2 hsa-miR-376a 2.90 4.04 1.83 3.57 0.860 2.26E−02 2.57 5.9 hsa-miR-381 3.48 1.28 3.22 3.08 0.91 0 2.37E−02 2.696.4 hsa-miR-19a 1.60 1.28 2.51 2.82 1.01 0 3.81E−02 2.64 6.3 hsa-miR-4103.15 1.85 2.51 2.51 0.68 0 6.32E−03 2.64 6.2 hsa-miR-503 6.39 6.60 5.996.62 0.46 78 6.90E−04 2.62 6.1 hsa-miR-503 6.85 6.87 6.74 6.87 0.30 1008.33E−05 2.31 5.0 hsa-asg-6377_st2 2.77 2.41 3.06 2.67 0.66 0 5.85E−032.61 6.1 hsa-asg-12907_st2 2.77 1.66 2.51 2.69 0.66 0 6.08E−03 2.58 6.0hsa-miR-499 4.80 4.55 3.84 4.87 0.85 22 2.10E−02 2.58 6.0 hsa-miR-4995.03 4.25 4.58 4.86 0.74 22 1.46E−02 2.42 5.3 hsa-asg-14146_st1 5.784.66 6.46 5.82 0.68 78 7.10E−03 2.57 5.9 hsa-miR-218 7.75 7.43 8.74 8.770.77 100 1.35E−02 2.56 5.9 hsa-cand206_st1 3.57 1.28 2.71 2.65 0.86 02.23E−02 2.56 5.9 hsa-miR-660 7.78 7.90 8.59 8.51 0.55 100 2.38E−03 2.555.9 hsa-miR-362 6.93 6.55 7.04 7.30 0.43 100 5.28E−04 2.52 5.7hsa-miR-9* 4.92 4.71 5.20 5.55 0.53 44 2.12E−03 2.51 5.7 hsa-miR-5771.96 2.05 3.22 2.78 0.92 0 3.37E−02 2.49 5.6 hsa-asg-9681_st1 4.05 3.294.51 4.13 0.75 0 1.35E−02 2.48 5.6 hsa-miR-369-5p 3.38 2.74 3.50 3.360.56 0 3.05E−03 2.48 5.6 hsa-miR-369-5p 2.90 2.58 3.50 3.42 0.81 02.04E−02 2.45 5.5 hsa-asg-14230_st1 2.77 3.93 4.04 3.82 0.58 0 3.81E−032.47 5.5 hsa-asg-13908_st2 2.62 2.41 3.73 2.63 0.72 0 1.22E−02 2.45 5.5hsa-asg-11361_st2 3.83 2.58 4.29 3.39 0.52 0 2.16E−03 2.42 5.4hsa-miR-500 8.11 8.41 8.49 8.41 0.35 100 1.69E−04 2.40 5.3hsa-asg-9574_st2 5.03 6.54 6.00 5.84 0.77 44 1.87E−02 2.38 5.2hsa-miR-592 3.03 2.58 4.51 2.94 0.96 0 4.73E−02 2.36 5.1 hsa-miR102_st27.93 8.39 8.40 8.35 0.37 100 3.38E−04 2.35 5.1 hsa-miR-30e-5p 9.21 8.139.48 9.56 0.71 100 1.39E−02 2.33 5.0 hsa-asg-1268_st1 3.83 2.23 3.623.67 0.69 0 1.29E−02 2.32 5.0 hsa-asg-128_st2 4.96 4.32 4.13 4.58 0.35 02.85E−04 2.27 4.8 hsa-asg-13997_st2 1.25 3.17 2.29 2.51 0.65 0 1.17E−022.23 4.7 hsa-asg-548_st1 3.38 2.74 4.58 3.05 0.83 0 3.41E−02 2.22 4.7hsa-asg-12552_st1 3.83 4.61 6.27 4.23 0.86 11 4.00E−02 2.22 4.7hsa-asg-11612_st1 4.62 5.25 6.01 5.07 0.51 11 3.42E−03 2.21 4.6hsa-asg-11338_st2 3.15 4.12 5.44 4.38 0.81 11 3.60E−02 2.15 4.4hsa-asg-6642_st2 2.90 2.89 3.73 3.63 0.48 0 3.13E−03 2.12 4.4hsa-asg-1221_st2 2.62 2.89 3.50 3.52 0.61 0 1.08E−02 2.12 4.3hsa-asg-7471_st1 5.24 4.97 6.07 5.65 0.52 56 5.03E−03 2.10 4.3hsa-miR-154* 4.25 3.17 4.37 4.31 0.79 11 3.65E−02 2.09 4.2hsa-asg-1596_st2 4.12 4.19 4.37 4.20 0.49 0 3.90E−03 2.07 4.2hsa-miR-183 7.50 6.30 6.63 6.97 0.72 89 2.57E−02 2.06 4.2 hsa-miR-148b9.07 8.12 9.12 9.16 0.71 100 2.50E−02 2.06 4.2 hsa-asg-9526_st2 5.846.65 6.83 5.92 0.75 78 3.34E−02 2.03 4.1 hsa-miR-455 5.07 5.30 4.58 5.230.66 33 2.09E−02 1.99 4.0 hsa-asg-6381_st1 3.27 3.40 4.64 3.85 0.76 03.87E−02 1.98 3.9 hsa-asg-9687_st1 4.05 3.71 3.50 4.15 0.47 0 4.17E−031.96 3.9 hsa-asg-8731_st2 3.83 5.36 4.76 4.45 0.52 0 7.29E−03 1.94 3.8hsa-asg-5986_st1 8.00 7.41 8.39 7.18 0.75 0 4.09E−02 1.93 3.8hsa-miR-363 10.0 9.10 9.93 9.79 0.53 100 8.21E−03 1.93 3.8hsa-asg-4608_st1 3.98 5.01 4.97 4.46 0.56 0 1.33E−02 1.86 3.6hsa-miR-487a 3.66 3.71 3.62 3.72 0.20 0 2.40E−05 1.84 3.6hsa-asg-4637_st1 6.05 6.48 5.91 6.42 0.33 100 6.92E−04 1.84 3.6hsa-miR-494 5.71 5.84 6.58 6.68 0.64 100 2.65E−02 1.84 3.6 hsa-miR-4945.38 5.77 6.87 6.45 0.55 89 4.40E−02 1.39 2.6 hsa-miR-324-5p 7.80 7.857.97 7.93 0.29 100 3.15E−04 1.83 3.5 hsa-asg-12666_st2 4.80 3.97 4.824.80 0.55 11 1.43E−02 1.82 3.5 hsa-miR-19b 6.90 6.51 7.18 7.45 0.74 1004.98E−02 1.81 3.5 hsa-asg-13279_st1 6.86 6.98 6.66 7.22 0.52 1001.06E−02 1.81 3.5 hsa-asg-10775_st2 4.80 4.50 4.70 4.73 0.24 0 1.06E−041.81 3.5 hsa-asg-10626_st2 4.84 4.25 4.97 4.84 0.39 0 2.48E−03 1.78 3.4hsa-asg-12825_st1 3.75 3.71 3.06 3.53 0.69 0 4.15E−02 1.77 3.4hsa-asg-3998_st1 9.20 8.74 8.47 8.75 0.35 100 1.48E−03 1.74 3.3hsa-miR-510 4.52 4.25 5.07 4.81 0.32 11 8.75E−04 1.73 3.3hsa-asg-9053_st1 4.88 5.90 5.62 5.85 0.69 33 4.67E−02 1.70 3.2hsa-miR-518c* 3.66 3.71 4.64 4.02 0.65 0 4.49E−02 1.64 3.1hsa-asg-3503_st2 3.15 4.66 4.44 3.67 0.57 0 2.57E−02 1.63 3.1hsa-miR-130a 10.0 9.61 10.47 10.10 0.57 100 2.68E−02 1.63 3.1hsa-miR-130a 9.99 9.54 10.59 10.11 0.60 100 3.45E−02 1.61 3.0hsa-asg-10336_st1 4.36 3.71 4.04 4.11 0.42 0 6.10E−03 1.63 3.1hsa-asg-2237_st1 4.12 4.19 4.70 4.38 0.40 0 4.99E−03 1.61 3.1hsa-asg-3046_st2 5.64 5.36 5.84 5.62 0.54 78 2.16E−02 1.61 3.0hsa-miR-329 3.98 2.58 3.50 3.58 0.59 0 3.35E−02 1.60 3.0hsa-asg-13298_st1 6.00 5.48 6.20 5.72 0.50 44 1.60E−02 1.60 3.0hsa-asg-3289_st1 2.47 3.17 3.94 3.49 0.56 0 2.67E−02 1.59 3.0hsa-asg-12922_st1 3.98 4.75 4.92 4.43 0.53 0 2.25E−02 1.58 3.0hsa-asg-4416_st1 3.91 2.89 2.51 3.60 0.56 0 2.94E−02 1.55 2.9hsa-asg-10830_st2 3.38 3.61 3.84 3.89 0.34 0 2.43E−03 1.55 2.9hsa-asg-1301_st1 4.18 3.80 2.89 3.78 0.43 0 9.32E−03 1.55 2.9hsa-asg-10298_st1 4.25 3.71 3.84 4.07 0.29 0 9.22E−04 1.55 2.9hsa-miR-422a 9.67 9.22 9.12 9.20 0.33 100 2.23E−03 1.54 2.9hsa-asg-14051_st2 6.09 6.95 6.96 6.57 0.55 67 3.13E−02 1.51 2.9hsa-miR257_st1 8.48 8.13 7.89 8.81 0.61 100 4.66E−02 1.50 2.8hsa-miR-106b 9.19 9.08 9.75 9.46 0.61 100 4.81E−02 1.50 2.8 hsa-miR-2106.09 6.46 7.00 6.59 0.50 89 2.21E−02 1.48 2.8 hsa-asg-7278_st1 3.98 4.735.42 5.02 0.58 11 4.13E−02 1.48 2.8 hsa-asg-8512_st2 3.48 4.32 4.44 4.020.46 0 1.85E−02 1.44 2.7 hsa-miR-422b 10.9 10.7 10.53 10.71 0.28 1001.30E−03 1.44 2.7 hsa-miR-181a* 5.30 5.77 6.53 5.80 0.43 56 1.40E−021.43 2.7 hsa-miR-379 6.33 6.13 6.20 6.58 0.37 100 6.27E−03 1.43 2.7hsa-miR-30a-5p 11.5 10.8 11.81 11.85 0.48 100 2.33E−02 1.41 2.7hsa-miR-370 4.31 4.47 5.09 4.84 0.36 0 6.58E−03 1.37 2.6hsa-asg-8160_st2 6.27 7.28 7.63 7.11 0.52 100 4.14E−02 1.32 2.5hsa-asg-13135_st1 5.14 4.44 5.57 5.11 0.44 22 2.15E−02 1.31 2.5hsa-miR-151 9.02 8.50 9.03 9.35 0.50 100 3.93E−02 1.30 2.5 hsa-miR-9810.8 10.1 10.65 10.99 0.44 100 2.36E−02 1.29 2.5 hsa-asg-4693_st1 4.525.65 5.54 5.14 0.51 11 4.59E−02 1.28 2.4 hsa-asg-9629_st2 3.98 4.75 5.164.77 0.46 0 2.94E−02 1.27 2.4 hsa-asg-2592_st1 6.42 6.43 6.57 6.50 0.4289 2.22E−02 1.26 2.4 hsa-asg-225_st1 4.84 5.76 5.22 5.57 0.48 223.94E−02 1.26 2.4 hsa-asg-2402_st1 4.80 3.97 3.73 3.93 0.47 0 4.01E−021.22 2.3 hsa-miR-421 7.76 8.76 8.05 8.19 0.36 100 1.29E−02 1.21 2.3hsa-miR-421 8.36 9.37 8.90 9.02 0.38 100 2.86E−02 1.06 2.1hsa-asg-7131_st1 6.28 7.49 7.39 6.93 0.48 33 4.85E−02 1.18 2.3hsa-miR-30d 12.0 11.6 12.52 12.32 0.44 100 3.62E−02 1.17 2.3hsa-miR-454-3p 7.89 7.31 7.80 8.06 0.43 100 3.45E−02 1.15 2.2hsa-asg-12697_st1 3.75 4.04 4.04 3.92 0.34 0 1.32E−02 1.13 2.2hsa-asg-3156_st1 4.42 5.23 5.24 4.90 0.38 11 2.25E−02 1.12 2.2hsa-asg-10007_st1 6.98 7.78 7.69 7.34 0.42 56 3.44E−02 1.12 2.2hsa-asg-14233_st1 9.03 8.85 8.87 9.23 0.41 100 3.37E−02 1.12 2.2hsa-asg-5385_st2 5.20 5.66 5.86 5.80 0.40 33 3.41E−02 1.08 2.1hsa-asg-7713_st2 4.80 5.72 5.11 4.88 0.41 0 4.86E−02 1.00 2.0 SD,standard deviation; %, percentage of samples expressing miRNA abovebackground levels; Log2Diff, difference in VSN-transformed expressionbewteen KID and LNneg samples.

Example 29 miRNAs Differentally Expressed Between Normal Stomach Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous stomach cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal stomachtissue than in cancer-negative lymph nodes.

Total RNA, purified from one normal human stomach sample (FirstChoice®Human Stomach Total RNA, cat. no. AM7996; Ambion, Austin, Tex., USA) andfrom nine normal human lymph node samples (FirstChoice® Human Lymph NodeTotal RNA, cat. no. AM7894; Ambion, Austin, Tex., USA) was purchasedfrom Ambion. miRNA purification and labeling and microarray expressionanalyses were performed as described above in Example 2.

The inventors identified 99 miRNAs (35 verified, 64 predicted) withsignificant differential expression between the normal stomach sampleand normal lymph nodes (|log 2Diff| stomach vs LN neg≧1 andp-value<0.05) (Table 28). Among the verified miRNAs, hsa-miR-375 showedan expression level over 600-fold higher in the stomach sample;hsa-miR-429 and -200a were expressed at levels between 100- and 200-foldhigher in the stomach sample; 7 miRNAs (hsa-miR-205, -200b, -203, -141,-200a*, -200c and -605) were expressed between 20- and 90-fold higher instomach; 6 miRNAs were expressed between 10- and 20-fold higher instomach; and 11 miRNAs were expressed between 5- and 10-fold higher inthe stomach sample than in normal lymph node samples. Among thepredicted miRNAs, hsa-asg-14153-st2 was expressed at a level over100-fold higher in the stomach sample; 5 miRNAs (hsa-asg-7637_st2,-924_st1, -11715_st2, -14182_st1, and -12227_st1) were expressed between20- and 60-fold higher in stomach; 14 miRNAs were expressed between 10-and 20-fold higher in stomach; and 21 miRNAs were expressed between 5-and 10-fold higher in the stomach sample than in normal lymph nodes.

TABLE 28 MicroRNAs Differentially Expressed Between Normal Stomach (STO)and Cancer-Negative Lymph Node Samples (LNneg) from Patients. ProbeLNneg MicroRNA ID STO 1 2 3 4 5 6 hsa-miR-375 hsa-miR-375_st1 13.69 5.954.88 6.24 2.82 2.93 3.63 hsa-miR-429 hsa-miR-429_st1 10.27 3.42 1.543.66 2.10 2.33 2.65 hsa-miR-200a hsa-miR-200a_st2 12.33 6.26 5.02 6.495.71 5.25 4.54 hsa-asg-14153 hsa-asg-14153_st2 9.50 3.05 2.23 3.04 2.982.33 2.65 hsa-miR-205 hsa-miR-205_st1 11.12 2.90 5.86 5.64 2.00 3.556.06 hsa-miR-200b hsa-miR-200b_st1 13.65 7.61 7.75 8.91 6.69 6.79 6.82hsa-asg-7637 hsa-asg-7637_st2 9.67 3.64 2.50 4.46 3.44 3.89 3.97hsa-miR-203 hsa-miR-203_st2 12.59 7.32 5.84 7.22 8.24 6.42 5.36hsa-miR-141 hsa-miR-141_st1 9.62 5.05 3.78 4.93 4.41 4.36 2.80hsa-asg-924 hsa-asg-924_st1 8.68 4.64 4.25 3.38 4.44 2.65 2.73hsa-miR-200a* hsa-miR-200a-_st2 7.81 3.05 2.83 2.15 3.89 2.57 1.76hsa-miR-200c hsa-miR-200c_st1 14.11 9.59 8.79 10.31 8.17 8.12 8.08hsa-asg-11715 hsa-asg-11715_st2 6.69 1.83 2.32 2.15 1.48 2.07 1.76hsa-miR-605 hsa-miR-605_st1 7.12 2.58 2.13 3.04 0.73 1.71 2.14hsa-asg-14182 hsa-asg-14182_st1 6.35 2.41 0.97 1.89 1.79 2.57 1.76hsa-asg-12227 hsa-asg-12227_st1 6.39 1.63 0.80 2.15 1.18 1.89 1.95hsa-miR-31 hsa-miR-31_st2 13.42 10.40 9.74 8.95 10.15 8.68 8.54hsa-asg-2424 hsa-asg-2424_st1 5.91 2.22 1.15 1.12 1.79 1.27 1.86hsa-asg-12139 hsa-asg-12139_st2 6.35 2.75 2.32 2.63 2.30 1.62 2.14hsa-miR-215 hsa-miR-215_st1 9.68 6.95 5.77 5.71 6.66 5.51 4.79hsa-asg-5886 hsa-asg-5886_st2 6.05 2.22 2.67 2.63 1.69 2.65 1.76hsa-miR-194 hsa-miR-194_st2 12.91 9.82 9.32 8.92 9.34 8.92 8.29hsa-miR-592 hsa-miR-592_st1 6.84 4.29 2.50 2.84 3.12 1.80 1.76hsa-miR-592 hsa-asg-4400_st1 6.42 3.05 3.24 3.66 1.69 1.62 2.65hsa-asg-10131 hsa-asg-10131_st2 7.11 3.31 3.11 3.52 2.48 2.49 3.20hsa-asg-13997 hsa-asg-13997_st2 6.36 3.05 3.24 2.15 2.98 2.16 2.32hsa-asg-11463 hsa-asg-11463_st1 5.74 2.03 2.13 2.63 1.69 1.62 1.95hsa-asg-8428 hsa-asg-8428_st2 5.63 2.22 1.94 2.63 2.00 2.49 1.03hsa-asg-5119 hsa-asg-5119_st1 6.33 2.41 2.67 1.89 1.90 1.98 2.49hsa-asg-13705 hsa-asg-13705_st1 6.08 1.63 0.97 3.78 2.30 2.65 2.32hsa-miR-192 hsa-miR-192_st2 13.40 10.83 9.82 10.13 10.00 9.55 8.66hsa-miR-96 hsa-miR-96_st1 6.97 4.97 3.36 3.04 2.98 4.07 2.80hsa-asg-9219 hsa-asg-9219_st2 5.56 2.58 1.74 2.15 2.57 1.80 1.86hsa-cand317 hsa-cand317_st1 5.93 3.42 2.67 1.63 3.12 1.10 1.57hsa-asg-4219 hsa-asg-4219_st1 5.92 3.42 1.74 1.63 1.48 2.24 1.76hsa-asg-3979 hsa-asg-3979_st1 5.53 2.41 2.83 2.84 2.30 2.16 2.65hsa-asg-4978 hsa-asg-4978_st2 5.83 1.63 2.83 2.84 1.69 1.98 1.95hsa-asg-1296 hsa-asg-1296_st1 6.54 3.05 2.50 4.81 2.39 2.49 2.73hsa-asg-11380 hsa-asg-11380_st2 5.72 2.90 1.74 3.04 2.48 2.16 1.95hsa-asg-3822 hsa-asg-3822_st2 5.97 2.03 2.97 3.66 2.00 2.41 2.14hsa-asg-14208 hsa-asg-14208_st1 6.88 5.05 3.48 4.46 4.14 3.35 1.21hsa-asg-10247 hsa-asg-10247_st1 5.74 2.75 2.13 4.01 1.69 2.16 2.32hsa-miR-487b hsa-miR-487b_st2 8.81 6.05 5.79 6.97 6.90 6.22 5.34hsa-miR-187 hsa-miR-187_st1 6.51 4.74 2.75 2.74 3.75 3.18 3.32hsa-asg-1495 hsa-asg-1495_st2 5.11 1.05 1.94 1.63 1.69 1.10 2.14hsa-asg-7464 hsa-asg-7464_st2 6.66 4.59 3.86 4.81 2.48 3.97 2.14hsa-cand852 hsa-cand852_st2 5.81 3.05 1.94 2.84 2.98 2.24 2.65hsa-asg-11361 hsa-asg-11361_st2 6.47 2.75 3.48 3.38 3.25 3.59 3.32hsa-asg-13908 hsa-asg-13908_st2 5.69 2.03 2.50 3.66 2.48 1.44 2.80hsa-miR-183 hsa-miR-183_st1 10.02 7.65 6.71 6.71 7.93 7.54 5.78hsa-miR-576 hsa-miR-576_st2 5.35 2.22 2.83 3.04 1.69 1.98 1.95hsa-asg-280 hsa-asg-280_st1 6.01 3.18 2.67 4.46 2.10 2.49 2.14hsa-miR-539 hsa-miR-539_st1 5.53 2.03 3.11 2.63 3.38 2.65 2.94hsa-asg-1268 hsa-asg-1268_st1 6.63 4.74 3.24 4.01 4.14 3.45 3.72hsa-miR-338 hsa-miR-338_st1 7.22 5.09 4.03 5.57 4.85 4.00 2.14hsa-asg-11160 hsa-asg-11160_st2 6.13 2.75 3.11 3.38 3.05 2.86 2.65hsa-asg-3251 hsa-asg-3251_st1 6.36 4.22 4.11 4.01 2.82 3.50 3.89hsa-asg-8174 hsa-asg-8174_st1 6.02 3.31 4.03 2.84 2.98 2.86 3.20hsa-miR-7 hsa-miR-7_st1 11.40 9.45 8.18 9.71 8.61 8.16 7.65 hsa-miR-182hsa-miR-182_st1 12.01 10.22 9.98 8.86 9.82 9.35 9.50 hsa-cand26hsa-cand26_st2 7.65 6.11 5.56 6.04 4.06 3.64 4.70 hsa-miR-381hsa-miR-381_st2 5.64 2.75 2.32 4.21 3.38 4.17 2.94 hsa-asg-1596hsa-asg-1596_st2 6.73 4.41 5.06 4.01 3.19 4.36 4.12 hsa-miR-130bhsa-miR-130b_st1 10.45 8.12 8.01 8.96 7.74 7.49 7.29 hsa-asg-548hsa-asg-548_st1 5.56 3.05 1.94 3.52 3.50 2.79 1.95 hsa-asg-13135hsa-asg-13135_st1 7.61 5.61 5.61 5.24 4.89 4.76 4.70 hsa-asg-12666hsa-asg-12666_st2 7.29 6.07 4.61 4.87 4.89 4.62 4.54 hsa-asg-3711hsa-asg-3711_st2 7.65 5.69 6.12 5.12 5.52 5.11 5.20 hsa-miR-376ahsa-asg-10049_st1 7.25 5.01 4.85 6.09 5.55 5.31 4.05 hsa-asg-13166hsa-asg-13166_st2 6.14 4.47 3.36 4.87 4.94 4.07 4.12 hsa-asg-10881hsa-asg-10881_st1 7.38 5.71 4.98 6.06 5.43 4.76 5.40 hsa-asg-2299hsa-asg-2299_st1 5.72 4.69 4.11 4.54 3.60 3.40 3.53 hsa-miR-494hsa-miR-494_st1 8.79 6.39 7.25 7.23 6.95 6.60 7.61 hsa-miR-494hsa-miR61_st2 7.95 6.81 6.50 6.86 6.42 6.35 7.05 hsa-cand581hsa-cand581_st1 5.46 3.83 3.58 3.66 3.89 3.68 2.65 hsa-miR-329hsa-miR-329_st1 5.43 3.73 2.97 4.61 3.25 4.54 2.80 hsa-miR-329hsa-asg-13204_st2 5.32 4.07 2.83 4.21 3.38 4.20 3.43 hsa-asg-13074hsa-asg-13074_st2 5.72 4.47 3.95 3.66 4.65 2.93 3.20 hsa-asg-12240hsa-asg-12240_st1 5.80 4.15 3.11 3.90 4.06 4.86 2.65 hsa-asg-7471hsa-asg-7471_st1 7.46 5.58 5.67 5.68 6.74 5.67 5.25 hsa-asg-7540hsa-asg-7540_st2 5.79 3.73 4.38 3.04 3.50 4.11 4.92 hsa-asg-6642hsa-asg-6642_st2 5.39 4.00 3.78 4.38 3.85 3.68 3.48 hsa-asg-6883hsa-asg-6883_st1 6.03 4.59 4.18 5.09 4.44 3.59 4.35 hsa-asg-10360hsa-asg-10360_st1 5.02 3.42 3.36 3.04 4.65 3.68 3.20 hsa-asg-10775hsa-asg-10775_st2 6.42 5.09 4.71 4.81 5.05 4.33 4.62 hsa-miR-379hsa-miR-379_st1 8.15 6.51 6.37 7.12 6.96 7.01 6.54 hsa-asg-12271hsa-asg-12271_st1 5.51 3.83 4.81 3.78 3.55 3.55 4.88 hsa-miR-503hsa-miR-503_st1 8.17 6.88 7.17 7.38 6.60 6.08 6.45 hsa-miR-503hsa-miR141_st1 8.42 7.10 7.28 7.30 6.52 6.55 6.62 hsa-asg-5018hsa-asg-5018_st1 5.46 4.79 3.78 3.21 4.62 3.77 3.85 hsa-asg-8290hsa-asg-8290_st1 9.45 7.95 8.42 6.86 7.93 8.76 8.29 hsa-asg-6924hsa-asg-6924_st1 8.05 6.53 7.33 6.10 7.10 6.12 6.92 hsa-miR-566hsa-miR-566_st1 7.35 6.72 6.12 5.12 5.83 6.11 6.10 hsa-asg-7130hsa-asg-7130_st1 6.84 4.93 6.25 4.65 5.34 5.51 6.13 hsa-miR-660hsa-miR-660_st1 9.95 9.28 8.94 7.94 9.15 8.60 8.38 hsa-miR-487ahsa-asg-6915_st1 5.11 4.07 3.58 3.90 3.38 3.85 3.72 hsa-asg-4745hsa-asg-4745_st2 6.60 4.35 4.98 5.81 5.15 5.12 5.40 hsa-asg-1301hsa-asg-1301_st1 5.11 4.47 3.68 3.90 3.71 3.77 3.63 hsa-asg-3604hsa-asg-3604_st1 6.78 5.88 5.79 5.98 5.79 5.75 5.15 hsa-asg-3722hsa-asg-3722_st1 9.99 9.12 9.51 9.24 8.61 8.85 8.99 hsa-miR-628hsa-miR-628_st2 8.65 8.29 7.83 7.21 8.00 7.32 7.47 hsa-asg-10229hsa-asg-10229_st2 7.03 5.81 5.77 6.17 6.84 6.05 5.96 hsa-asg-10065hsa-asg-10065_st1 5.15 4.47 4.31 3.66 4.70 4.44 3.63 LNneg MicroRNA 7 89 M SD % ttest log2Diff Fold hsa-miR-375 1.00 4.61 6.37 4.27 1.82 01.18E−03 −9.42 683 hsa-miR-429 2.31 2.41 5.36 2.86 1.14 0 2.69E−04 −7.40169 hsa-miR-200a 4.05 4.75 7.76 5.54 1.15 67 5.06E−04 −6.79 110hsa-asg-14153 1.17 2.74 4.37 2.73 0.85 0 6.65E−05 −6.77 109 hsa-miR-2053.38 5.30 7.74 4.71 1.85 33 1.10E−02 −6.41 85.0 hsa-miR-200b 6.50 7.6410.41 7.68 1.27 100 2.10E−03 −5.97 62.7 hsa-asg-7637 3.27 3.61 5.24 3.780.77 0 8.63E−05 −5.89 59.3 hsa-miR-203 5.17 6.05 9.34 6.77 1.38 784.01E−03 −5.82 56.5 hsa-miR-141 3.83 3.80 4.51 4.16 0.69 0 7.15E−05−5.45 43.8 hsa-asg-924 2.14 2.74 3.50 3.38 0.89 0 5.00E−04 −5.30 39.4hsa-miR-200a* 2.14 3.17 2.89 2.72 0.64 0 6.89E−05 −5.10 34.2hsa-miR-200c 7.96 8.87 11.51 9.05 1.21 100 4.20E−03 −5.06 33.4hsa-asg-11715 0.63 1.46 3.36 1.90 0.74 0 2.80E−04 −4.79 27.7 hsa-miR-6052.90 3.03 4.44 2.52 1.03 0 2.88E−03 −4.60 24.2 hsa-asg-14182 2.77 1.850.73 1.86 0.68 0 2.43E−04 −4.49 22.5 hsa-asg-12227 2.77 2.58 2.71 1.960.68 0 2.64E−04 −4.43 21.5 hsa-miR-31 7.71 8.66 10.09 9.21 0.92 1002.40E−03 −4.21 18.5 hsa-asg-2424 2.62 1.66 2.89 1.84 0.63 0 2.98E−04−4.07 16.7 hsa-asg-12139 1.60 2.05 3.50 2.32 0.59 0 1.92E−04 −4.02 16.3hsa-miR-215 5.49 5.09 5.20 5.69 0.71 33 6.86E−04 −3.99 15.9 hsa-asg-58860.78 2.41 1.83 2.07 0.62 0 3.05E−04 −3.98 15.8 hsa-miR-194 8.54 8.209.03 8.93 0.53 100 9.77E−05 −3.98 15.8 hsa-miR-592 3.03 2.58 4.51 2.940.96 0 4.78E−03 −3.90 14.9 hsa-miR-592 3.03 1.66 5.40 2.89 1.21 112.43E−02 −3.53 11.6 hsa-asg-10131 2.62 3.17 5.07 3.22 0.79 0 1.58E−03−3.89 14.8 hsa-asg-13997 1.25 3.17 2.29 2.51 0.65 0 5.02E−04 −3.85 14.4hsa-asg-11463 2.31 1.66 1.14 1.91 0.44 0 3.30E−05 −3.84 14.3hsa-asg-8428 0.04 2.14 1.60 1.79 0.81 0 2.01E−03 −3.84 14.3 hsa-asg-51192.90 2.58 3.62 2.49 0.55 0 1.77E−04 −3.83 14.2 hsa-asg-13705 0.37 3.173.22 2.27 1.11 0 1.15E−02 −3.81 14.0 hsa-miR-192 9.31 8.79 9.63 9.640.67 100 7.18E−04 −3.77 13.6 hsa-miR-96 3.03 1.85 3.06 3.24 0.87 113.53E−03 −3.72 13.2 hsa-asg-9219 1.42 1.28 2.29 1.97 0.47 0 8.50E−05−3.60 12.1 hsa-cand317 3.75 1.85 2.51 2.40 0.92 0 6.52E−03 −3.53 11.6hsa-asg-4219 2.90 2.74 3.94 2.43 0.87 0 5.30E−03 −3.49 11.2 hsa-asg-39790.26 2.23 1.83 2.17 0.79 0 3.70E−03 −3.36 10.3 hsa-asg-4978 2.77 2.584.13 2.49 0.79 0 3.77E−03 −3.34 10.1 hsa-asg-1296 3.15 3.88 4.13 3.240.85 0 6.24E−03 −3.30 9.9 hsa-asg-11380 2.47 2.23 3.06 2.45 0.48 01.86E−04 −3.27 9.6 hsa-asg-3822 1.60 2.89 4.76 2.72 0.99 0 1.42E−02−3.25 9.5 hsa-asg-14208 3.03 4.04 3.94 3.63 1.09 0 2.25E−02 −3.25 9.5hsa-asg-10247 0.37 3.17 3.94 2.50 1.14 0 2.68E−02 −3.24 9.5 hsa-miR-487b4.88 3.71 4.37 5.58 1.11 67 2.48E−02 −3.23 9.4 hsa-miR-187 3.03 3.353.14 3.33 0.61 0 1.18E−03 −3.17 9.0 hsa-asg-1495 2.90 2.23 2.89 1.950.67 0 2.13E−03 −3.16 8.9 hsa-asg-7464 3.66 2.23 3.84 3.51 0.99 01.68E−02 −3.15 8.9 hsa-cand852 2.90 2.05 3.50 2.68 0.51 0 4.14E−04 −3.138.8 hsa-asg-11361 3.83 2.58 4.29 3.39 0.52 0 4.78E−04 −3.09 8.5hsa-asg-13908 2.62 2.41 3.73 2.63 0.72 0 3.87E−03 −3.06 8.3 hsa-miR-1837.50 6.30 6.63 6.97 0.72 89 3.71E−03 −3.05 8.3 hsa-miR-576 1.96 2.053.06 2.31 0.52 0 5.52E−04 −3.05 8.3 hsa-asg-280 1.78 3.40 4.51 2.97 1.000 2.06E−02 −3.04 8.2 hsa-miR-539 1.96 2.58 1.36 2.52 0.63 0 1.95E−03−3.01 8.1 hsa-asg-1268 3.83 2.23 3.62 3.67 0.69 0 3.58E−03 −2.96 7.8hsa-miR-338 4.76 3.40 4.87 4.30 1.04 11 2.89E−02 −2.92 7.6 hsa-asg-111602.47 3.51 5.11 3.21 0.79 0 7.92E−03 −2.92 7.6 hsa-asg-3251 2.90 3.033.22 3.52 0.55 0 1.17E−03 −2.84 7.2 hsa-asg-8174 2.77 2.74 4.13 3.210.53 0 1.01E−03 −2.81 7.0 hsa-miR-7 8.64 8.56 8.46 8.60 0.64 1003.19E−03 −2.79 6.9 hsa-miR-182 8.97 8.56 8.11 9.26 0.70 100 5.62E−03−2.75 6.7 hsa-cand26 5.27 4.04 5.44 4.98 0.91 22 2.38E−02 −2.67 6.4hsa-miR-381 3.48 1.28 3.22 3.08 0.91 0 2.91E−02 −2.56 5.9 hsa-asg-15964.12 4.19 4.37 4.20 0.49 0 1.19E−03 −2.53 5.8 hsa-miR-130b 7.93 7.698.08 7.92 0.48 100 1.02E−03 −2.53 5.8 hsa-asg-548 3.38 2.74 4.58 3.050.83 0 2.04E−02 −2.51 5.7 hsa-asg-13135 5.14 4.44 5.57 5.11 0.44 226.12E−04 −2.51 5.7 hsa-asg-12666 4.80 3.97 4.82 4.80 0.55 11 2.76E−03−2.49 5.6 hsa-asg-3711 4.52 4.41 4.84 5.17 0.55 0 2.66E−03 −2.48 5.6hsa-miR-376a 3.83 4.12 4.64 4.83 0.75 11 1.56E−02 −2.43 5.4hsa-asg-13166 3.57 2.05 4.04 3.94 0.88 0 4.62E−02 −2.20 4.6hsa-asg-10881 4.18 5.01 5.20 5.19 0.55 22 5.36E−03 −2.19 4.6hsa-asg-2299 3.38 2.23 2.89 3.60 0.77 0 3.18E−02 −2.12 4.3 hsa-miR-4945.71 5.84 6.58 6.68 0.64 100 1.42E−02 −2.11 4.3 hsa-miR-494 5.38 5.776.87 6.45 0.55 89 3.27E−02 −1.50 2.8 hsa-cand581 3.83 2.41 3.22 3.420.54 0 7.27E−03 −2.04 4.1 hsa-miR-329 2.77 3.29 2.71 3.41 0.74 03.17E−02 −2.02 4.0 hsa-miR-329 3.98 2.58 3.50 3.58 0.59 0 2.39E−02 −1.743.3 hsa-asg-13074 3.38 3.80 3.62 3.74 0.56 0 1.01E−02 −1.98 3.9hsa-asg-12240 3.03 4.38 4.37 3.84 0.74 0 3.54E−02 −1.97 3.9 hsa-asg-74715.24 4.97 6.07 5.65 0.52 56 1.09E−02 −1.81 3.5 hsa-asg-7540 3.91 4.443.94 4.00 0.55 0 1.53E−02 −1.79 3.5 hsa-asg-6642 2.90 2.89 3.73 3.630.48 0 8.74E−03 −1.76 3.4 hsa-asg-6883 4.18 3.51 4.58 4.28 0.49 09.92E−03 −1.75 3.4 hsa-asg-10360 3.15 2.41 2.71 3.29 0.63 0 3.25E−02−1.73 3.3 hsa-asg-10775 4.80 4.50 4.70 4.73 0.24 0 1.73E−04 −1.69 3.2hsa-miR-379 6.33 6.13 6.20 6.58 0.37 100 3.70E−03 −1.57 3.0hsa-asg-12271 3.15 4.04 3.94 3.95 0.57 0 3.13E−02 −1.56 3.0 hsa-miR-5036.39 6.60 5.99 6.62 0.46 78 1.29E−02 −1.56 2.9 hsa-miR-503 6.85 6.876.74 6.87 0.30 100 1.21E−03 −1.55 2.9 hsa-asg-5018 3.38 4.12 3.73 3.920.52 0 2.25E−02 −1.54 2.9 hsa-asg-8290 7.45 7.99 7.59 7.92 0.56 1003.25E−02 −1.54 2.9 hsa-asg-6924 6.35 5.96 6.54 6.55 0.48 78 1.72E−02−1.50 2.8 hsa-miR-566 5.55 6.02 5.42 5.89 0.47 56 1.85E−02 −1.47 2.8hsa-asg-7130 4.67 5.44 5.49 5.38 0.57 22 4.15E−02 −1.46 2.7 hsa-miR-6607.78 7.90 8.59 8.51 0.55 100 3.84E−02 −1.44 2.7 hsa-miR-487a 3.66 3.713.62 3.72 0.20 0 1.80E−04 −1.39 2.6 hsa-asg-4745 5.27 4.97 6.05 5.230.49 44 2.99E−02 −1.37 2.6 hsa-asg-1301 4.18 3.80 2.89 3.78 0.43 01.93E−02 −1.33 2.5 hsa-asg-3604 5.71 5.30 5.28 5.63 0.30 78 6.55E−03−1.16 2.2 hsa-asg-3722 8.29 9.00 8.68 8.92 0.37 100 2.45E−02 −1.07 2.1hsa-miR-628 7.23 7.64 7.43 7.60 0.37 100 2.79E−02 −1.05 2.1hsa-asg-10229 5.47 5.69 6.02 5.98 0.39 100 3.35E−02 −1.05 2.1hsa-asg-10065 4.05 3.80 3.94 4.11 0.39 0 3.36E−02 −1.04 2.1 SD, standarddeviation; %, percentage of samples expressing miRNA above backgroundlevels; Log2Diff, difference in VSN-transformed expression bewteen STOand LNneg samples.

Example 30 miRNAs Differentally Expressed Between Normal Testicle Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous testes cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal testestissue than in cancer-negative lymph nodes.

Total RNA, purified from one normal human testicle sample (FirstChoice®Human Testicle Total RNA, cat. no.AM7972; Ambion, Austin, Tex., USA) andfrom nine normal human lymph node samples (FirstChoice® Human Lymph NodeTotal RNA, cat. no. AM7894; Ambion, Austin, Tex., USA) was purchasedfrom Ambion. miRNA purification and labeling and microarray expressionanalyses were performed as described above in Example 2.

The inventors identified 412 miRNAs (100 verified, 312 predicted) withsignificant differential expression between the normal testicle andnormal lymph node samples (|log 2Diff| testicle vs LN neg≧1 andp-value<0.05) (Table 29). Among the verified miRNAs, hsa-miR-513 wasexpressed at a level over 200-fold higher in the testicle sample than inthe lymh node samples; hsa-miR-514 and -509 were expressed at levelsbetween 100- and 200-fold higher in the testicle sample; 9 miRNAs(hsa-miR-493-5p, -506, -383, -411, -493-3p, -510, -187, -508 and -517a )were expressed at levels between 50- and 100-fold higher in the testiclesample; 27 miRNAs were expressed between 20- and 50-fold higher intesticle; 29miRNAs were expressed between 10- and 20-fold higher intesticle compared to LNneg; and 14 miRNAs were expressed between 5- and10-fold higher in the testicle sample than in normal lymph nodes. Amongthe predicted miRNAs, hsa-asg-5638_st2 was expressed at a level over800-fold higher in the testicle sample than in the normal lymph nodes;hsa-miR-5630_st2, -1088 st1 and -11338_st2) were expressed at levelsbetween 100- and 200-fold higher in the testicle sample; 6 miRNAs wereexpressed between 50- and 100-fold higher in testicle; 33 miRNAs wereexpressed between 20- and 50-fold higher in testicle; 59 miRNAs wereexpressed between 10- and 50-fold higher in testicle; and 97 miRNAs wereexpressed between 5- and 10-fold higher in the testicle sample than innormal lymph nodes.

TABLE 29 MicroRNAs Differentially Expressed Between Normal Testicle(TES) and Cancer-Negative Lymph Node Samples (LNneg) from Patients.Probe LNneg MicroRNA ID TES 1 2 3 4 5 6 hsa-asg-5638 hsa-asg-5638_st212.22 4.00 2.50 2.15 2.57 1.62 2.57 hsa-miR-513 hsa-miR-513_st1 13.116.17 6.89 4.99 5.44 4.00 5.87 hsa-asg-5630 hsa-asg-5630_st2 10.66 4.223.86 2.63 2.74 2.72 4.32 hsa-asg-1088 hsa-asg-1088_st1 9.34 1.83 2.131.63 1.59 2.07 0.87 hsa-miR-514 hsa-miR-514_st1 9.00 2.03 1.54 2.40 1.691.10 1.57 hsa-asg-11338 hsa-asg-11338_st2 11.53 4.07 5.59 3.52 4.28 4.364.92 hsa-miR-509 hsa-miR-509_st2 12.89 7.10 6.67 5.57 5.79 5.76 6.72hsa-miR-493-5p hsa-miR-493-5p_st1 11.20 4.35 3.78 6.89 6.18 6.30 3.63hsa-asg-6283 hsa-asg-6283_st1 10.67 3.83 5.43 3.30 4.59 6.32 5.16hsa-asg-13404 hsa-asg-13404_st2 9.17 4.15 2.67 5.28 2.90 3.24 1.57hsa-miR-506 hsa-miR-506_st2 12.43 5.76 5.53 6.78 6.12 6.23 5.96hsa-asg-14208 hsa-asg-14208_st1 9.60 5.05 3.48 4.46 4.14 3.35 1.21hsa-miR-383 hsa-miR-383_st1 8.42 3.31 2.83 2.40 2.57 3.18 1.30hsa-asg-10309 hsa-asg-10309_st1 10.18 5.24 5.74 2.15 4.65 6.00 4.96hsa-miR-411 hsa-miR-411_st1 9.77 4.84 3.68 4.54 5.22 5.52 2.94hsa-miR-493-3p hsa-asg-8480_st1 7.24 0.56 0.57 2.74 1.48 1.35 0.64hsa-miR-493-3p hsa-miR-493-3p_st1 7.39 4.64 3.05 4.06 2.98 2.24 1.86hsa-asg-7751 hsa-asg-7751_st2 9.20 2.90 3.68 3.38 2.74 5.03 5.07hsa-miR-510 hsa-miR-510_st2 10.58 5.27 5.02 4.75 4.65 4.71 5.04hsa-miR-187 hsa-miR-187_st1 9.09 4.74 2.75 2.74 3.75 3.18 3.32hsa-miR-508 hsa-miR-508_st1 11.90 5.71 5.93 6.66 6.63 6.16 6.36hsa-asg-13922 hsa-asg-13922_st1 9.55 4.69 5.35 1.63 4.82 5.55 5.11hsa-miR-517a hsa-miR-517a_st1 9.72 4.84 5.32 6.26 3.55 3.06 3.77hsa-miR-517a hsa-miR-517b_st1 9.76 4.97 5.56 6.51 3.85 3.18 3.77hsa-miR-299-5p hsa-miR-299-5p_st2 10.37 5.71 4.44 5.81 6.37 5.96 2.94hsa-miR202 hsa-miR202_st2 9.08 3.83 2.67 4.93 3.94 3.59 2.40hsa-asg-9514 hsa-asg-9514_st1 8.95 2.58 4.50 3.21 2.48 4.33 3.63hsa-asg-9510 hsa-asg-9510_st1 11.69 6.92 7.25 4.99 6.80 8.04 7.10hsa-miR-487b hsa-miR-487b_st2 11.04 6.05 5.79 6.97 6.90 6.22 5.34hsa-asg-5617 hsa-asg-5617_st1 8.19 3.73 3.24 3.90 3.60 3.40 2.65hsa-asg-9512 hsa-asg-9512_st1 11.62 6.41 6.89 4.61 6.57 7.78 7.00hsa-miR-539 hsa-miR-539_st1 7.87 2.03 3.11 2.63 3.38 2.65 2.94hsa-miR-539 hsa-miR177_st1 7.29 2.58 2.50 1.37 2.39 1.80 1.86hsa-miR-432 hsa-miR-432_st2 10.12 4.53 5.38 5.93 5.52 5.74 5.62hsa-asg-7472 hsa-asg-7472_st2 7.26 1.63 2.13 1.89 2.00 2.86 1.76hsa-miR-495 hsa-miR-495_st1 10.52 6.01 4.76 6.97 6.25 6.29 4.38hsa-asg-4037 hsa-asg-4037_st1 7.79 2.75 2.32 1.89 3.50 3.35 2.32hsa-asg-3081 hsa-asg-3081_st1 9.26 4.74 4.44 3.21 4.28 5.72 4.49hsa-asg-8486 hsa-asg-8486_st2 8.50 2.75 3.36 3.21 2.66 3.85 3.89hsa-miR-127 hsa-miR-127_st1 11.74 6.80 5.52 7.96 7.53 7.66 5.66hsa-asg-20 hsa-asg-20_st1 7.97 3.91 3.91 1.00 3.50 3.81 3.48hsa-asg-3743 hsa-asg-3743_st1 8.39 3.18 4.28 3.30 3.25 3.45 2.87hsa-miR-154 hsa-miR-154_st2 8.65 3.73 2.50 4.30 4.35 4.88 3.08hsa-asg-5021 hsa-asg-5021_st1 9.80 5.17 4.38 7.00 6.05 5.88 3.48hsa-miR-369-5p hsa-miR-369-5p_st2 8.56 3.53 2.67 4.46 4.35 4.33 2.49hsa-miR-369-5p hsa-asg-1521_st1 8.18 3.73 3.36 4.01 2.20 3.68 3.68hsa-asg-13166 hsa-asg-13166_st2 8.95 4.47 3.36 4.87 4.94 4.07 4.12hsa-asg-13801 hsa-asg-13801_st1 7.67 2.03 3.18 3.13 3.12 3.81 3.77hsa-miR-184 hsa-miR-184_st1 9.09 5.50 4.50 4.11 4.28 3.89 5.11hsa-cand443 hsa-cand443_st2 9.53 3.91 5.10 3.90 4.48 5.73 5.21hsa-miR-409-3p hsa-miR-409-3p_st1 8.66 2.22 3.42 5.14 4.98 4.73 2.94hsa-miR-134 hsa-miR-134_st2 10.07 5.09 5.73 6.00 5.84 5.67 4.15hsa-asg-13254 hsa-asg-13254_st1 9.77 5.58 5.32 6.66 5.89 5.87 3.97hsa-miR-376a hsa-miR-376a_st2 9.23 5.13 4.03 5.49 5.66 4.82 3.97hsa-miR-376a hsa-asg-10049_st1 9.62 5.01 4.85 6.09 5.55 5.31 4.05hsa-miR-376a hsa-asg-14234_st1 8.06 3.31 4.25 3.38 3.98 4.82 3.63hsa-asg-1020 hsa-asg-1020_st2 10.79 6.39 7.36 3.45 6.17 7.05 6.87hsa-miR-487a hsa-miR-487a_st2 7.33 2.22 2.83 2.63 3.38 2.16 3.20hsa-miR-379 hsa-miR-379_st1 11.39 6.51 6.37 7.12 6.96 7.01 6.54hsa-asg-3597 hsa-asg-3597_st2 11.40 6.62 7.72 5.07 6.87 7.74 6.93hsa-asg-8242 hsa-asg-8242_st2 7.90 2.75 3.53 1.37 4.35 4.20 4.43hsa-asg-3009 hsa-asg-3009_st1 9.51 5.17 5.57 4.34 5.20 5.40 5.00hsa-miR-204 hsa-miR-204_st2 10.92 7.17 5.77 5.98 8.20 6.73 5.40hsa-miR-381 hsa-miR-381_st2 7.81 2.75 2.32 4.21 3.38 4.17 2.94hsa-miR-520a* hsa-miR-520a-_st2 6.52 1.24 2.67 3.04 1.28 2.41 1.57hsa-asg-1608 hsa-asg-1608_st2 8.45 4.79 4.63 2.52 4.53 4.84 4.35hsa-miR-368 hsa-miR-368_st2 10.94 6.48 6.47 7.70 8.08 7.68 5.18hsa-miR-526a hsa-miR-526a_st2 7.45 2.75 3.36 4.75 1.90 2.24 2.80hsa-cand206 hsa-cand206_st1 7.33 2.22 1.94 3.78 3.60 2.65 2.14hsa-asg-3742 hsa-asg-3742_st1 8.67 3.83 4.90 3.66 4.25 4.84 3.43hsa-miR-202 hsa-miR-202_st1 9.46 4.69 5.02 4.38 4.96 5.02 5.58hsa-miR-485-5p hsa-miR-485-5p_st2 7.70 3.42 3.24 3.38 3.66 3.24 1.95hsa-miR-518f* hsa-miR-518f-_st2 7.14 2.03 2.13 4.68 2.66 2.24 1.95hsa-miR-494 hsa-miR-494_st1 11.26 6.39 7.25 7.23 6.95 6.60 7.61hsa-miR-494 hsa-miR61_st2 10.51 6.81 6.50 6.86 6.42 6.35 7.05hsa-miR-526c hsa-miR-526c_st1 7.48 3.53 2.67 5.04 2.66 1.53 2.94hsa-asg-677 hsa-asg-677_st2 8.61 3.42 4.66 3.90 4.14 5.03 4.74hsa-asg-5996 hsa-asg-5996_st2 11.22 8.09 7.62 5.28 7.67 8.00 6.94hsa-miR-520g hsa-miR-520g_st1 7.30 2.22 3.11 3.04 3.38 1.80 2.94hsa-asg-4756 hsa-asg-4756_st2 7.36 2.75 3.05 3.45 1.90 4.30 3.58hsa-miR-382 hsa-miR-382_st1 11.23 7.16 6.80 7.35 7.13 7.40 6.51hsa-miR-450 hsa-miR-450_st2 8.27 4.22 2.67 2.84 3.89 3.59 3.97hsa-miR-450 hsa-asg-14228_st2 7.21 3.53 2.13 2.40 2.98 2.49 3.08hsa-asg-12356 hsa-asg-12356_st1 11.87 7.94 8.57 5.85 7.74 8.64 8.25hsa-asg-8129 hsa-asg-8129_st2 7.72 3.05 3.91 2.28 3.12 4.30 4.72hsa-cand733 hsa-cand733_st1 8.33 6.03 4.21 2.03 4.82 5.94 5.04hsa-asg-6619 hsa-asg-6619_st1 7.13 2.58 0.57 2.03 4.70 2.86 4.22hsa-asg-8128 hsa-asg-8128_st1 7.18 3.42 3.99 3.45 2.98 3.35 2.87hsa-asg-14209 hsa-asg-14209_st1 8.81 4.59 4.90 5.68 3.75 3.06 3.48hsa-asg-7295 hsa-asg-7295_st2 6.76 2.90 2.13 3.38 1.59 2.07 2.49hsa-asg-10478 hsa-asg-10478_st2 7.70 3.05 4.03 3.38 2.48 4.38 4.12hsa-asg-7527 hsa-asg-7527_st2 8.48 4.59 5.00 2.74 3.12 4.92 4.81hsa-miR-449 hsa-miR-449_st2 6.95 2.58 1.54 4.54 3.05 3.59 1.67hsa-miR-503 hsa-miR-503_st1 10.85 6.88 7.17 7.38 6.60 6.08 6.45hsa-miR-503 hsa-miR141_st1 10.74 7.10 7.28 7.30 6.52 6.55 6.62hsa-cand91 hsa-cand91_st1 8.46 4.53 5.57 2.15 4.85 4.38 5.15 hsa-miR-608hsa-miR-608_st2 7.48 3.91 4.07 1.76 3.55 4.14 5.07 hsa-miR-135bhsa-miR-135b_st2 6.75 1.43 2.67 2.40 2.82 2.33 3.08 hsa-miR-135bhsa-cand78_st1 6.28 3.42 2.50 3.66 4.14 3.68 2.80 hsa-asg-13741hsa-asg-13741_st2 6.11 0.15 2.50 1.89 2.57 1.89 2.49 hsa-miR-105hsa-miR-105_st1 6.15 1.63 1.34 2.63 0.82 2.72 1.39 hsa-miR-433hsa-miR-433_st2 6.95 2.41 2.67 4.54 2.90 4.17 2.32 hsa-miR-377hsa-miR-377_st1 6.44 3.42 2.32 2.15 2.30 2.49 2.65 hsa-miR-572hsa-miR-572_st1 10.93 8.20 7.65 3.90 7.78 8.17 6.87 hsa-asg-13077hsa-asg-13077_st1 8.87 5.97 5.06 3.21 5.26 6.00 5.00 hsa-miR-516-5phsa-asg-13160_st1 6.94 2.41 2.97 3.90 2.74 2.49 2.05 hsa-miR-516-5phsa-miR-516-5p_st1 6.94 4.53 3.68 3.90 1.18 2.49 2.57 hsa-asg-9009hsa-asg-9009_st1 8.68 5.01 5.52 4.16 4.48 4.41 5.20 hsa-asg-3782hsa-asg-3782_st1 10.18 6.08 7.03 5.37 6.18 6.72 6.50 hsa-miR-409-5phsa-miR-409-5p_st1 9.70 5.13 4.98 6.68 6.08 6.08 5.11 hsa-miR-424hsa-miR-424_st1 9.76 5.79 6.11 5.53 5.91 5.75 3.43 hsa-asg-1089hsa-asg-1089_st1 6.32 3.31 1.74 2.40 2.66 2.93 1.57 hsa-miR-485-3phsa-miR23_st2 7.29 4.29 2.50 4.11 3.25 2.41 3.20 hsa-miR-485-3phsa-miR-485-3p_st2 7.24 3.64 2.97 4.30 4.41 4.24 3.08 hsa-miR-410hsa-miR-410_st1 6.57 2.41 2.32 1.37 3.71 2.49 2.80 hsa-asg-5149hsa-asg-5149_st2 8.90 5.31 4.92 4.71 4.82 5.40 5.21 hsa-asg-3672hsa-asg-3672_st1 9.22 5.34 6.49 4.16 5.36 5.72 5.99 hsa-miR-519dhsa-miR-519d_st1 6.61 1.43 2.32 3.78 2.57 2.49 2.87 hsa-asg-1199hsa-asg-1199_st1 6.81 2.22 3.95 2.84 2.74 3.24 2.32 hsa-asg-1619hsa-asg-1619_st2 7.29 4.35 3.24 2.84 4.80 3.12 2.80 hsa-miR-329hsa-asg-13589_st2 7.13 1.63 3.11 3.78 3.71 4.38 2.32 hsa-miR-329hsa-miR-329_st1 7.33 3.73 2.97 4.61 3.25 4.54 2.80 hsa-miR-329hsa-asg-13204_st2 7.21 4.07 2.83 4.21 3.38 4.20 3.43 hsa-asg-4283hsa-asg-4283_st2 7.14 3.91 3.48 2.63 3.80 3.89 2.80 hsa-cand930hsa-cand930_st1 7.47 3.31 4.52 3.96 1.90 4.11 3.14 hsa-asg-1881hsa-asg-1881_st2 7.93 3.42 5.30 2.28 3.75 4.07 5.21 hsa-miR-520hhsa-miR-520h_st1 6.54 3.18 2.67 3.21 1.90 2.65 2.80 hsa-miR-34chsa-miR-34c_st2 7.48 1.83 2.50 3.52 3.66 3.59 3.01 hsa-miR-376bhsa-miR-376b_st1 6.19 1.83 2.13 4.11 3.32 2.65 1.03 hsa-asg-9696hsa-asg-9696_st1 10.36 7.42 6.31 6.68 7.04 6.23 6.22 hsa-asg-13878hsa-asg-13878_st2 7.10 2.90 3.48 2.84 4.82 4.24 3.32 hsa-asg-3754hsa-asg-3754_st2 8.65 4.97 4.96 4.84 3.44 5.09 5.00 hsa-miR-515-3phsa-miR-515-3p_st2 5.32 2.41 0.97 1.37 1.28 1.71 1.39 hsa-miR-380-3phsa-miR-380-3p_st2 5.37 2.22 1.15 1.12 0.57 2.16 2.32 hsa-miR-380-3phsa-asg-13118_st1 5.04 2.03 3.11 3.04 1.28 1.62 2.32 hsa-cand561hsa-cand561_st1 6.86 2.41 3.53 1.24 2.30 3.24 4.15 hsa-asg-6815hsa-asg-6815_st1 9.05 4.97 6.29 4.21 5.13 5.58 6.22 hsa-asg-8616hsa-asg-8616_st2 7.21 3.42 4.44 1.63 4.53 3.55 4.19 hsa-asg-8630hsa-asg-8630_st2 9.10 5.52 6.35 3.45 5.78 5.98 6.65 hsa-cand150hsa-cand150_st2 7.36 6.26 3.68 2.40 2.48 3.12 3.43 hsa-asg-1203hsa-asg-1203_st1 7.46 4.47 4.31 2.63 3.80 4.17 4.49 hsa-asg-7159hsa-asg-7159_st2 7.09 3.53 3.24 2.40 3.94 3.89 3.43 hsa-asg-9262hsa-asg-9262_st1 8.20 4.59 4.28 3.96 5.74 4.71 4.52 hsa-asg-5606hsa-asg-5606_st1 6.39 2.41 3.99 2.74 2.10 1.89 3.68 hsa-asg-4872hsa-asg-4872_st1 7.25 3.31 4.50 3.04 3.85 2.65 4.22 hsa-asg-8893hsa-asg-8893_st1 8.38 4.97 4.92 4.71 4.80 5.44 4.98 hsa-asg-10947hsa-asg-10947_st1 6.98 3.64 3.48 1.63 5.09 3.73 3.63 hsa-asg-124hsa-asg-124_st1 8.67 4.88 5.28 4.30 4.56 5.70 5.34 hsa-asg-8987hsa-asg-8987_st1 7.22 2.22 4.78 2.74 3.44 3.50 4.96 hsa-asg-3740hsa-asg-3740_st1 8.71 4.88 6.41 4.68 5.07 5.43 5.88 hsa-miR223hsa-miR223_st2 8.99 5.50 6.08 4.96 4.70 5.14 6.01 hsa-asg-7671hsa-asg-7671_st1 9.85 6.24 7.39 5.76 6.13 6.82 6.90 hsa-asg-12735hsa-asg-12735_st1 10.03 6.81 6.64 5.85 6.49 7.38 7.02 hsa-asg-8138hsa-asg-8138_st2 9.23 6.41 6.52 4.42 5.36 6.40 6.34 hsa-miR-520fhsa-miR-520c_st2 5.75 2.41 1.34 1.89 1.69 2.16 2.14 hsa-miR-299-3phsa-miR-299-3p_st2 6.50 2.41 3.36 3.38 2.90 3.40 2.73 hsa-miR-299-3phsa-asg-10378_st1 6.30 3.64 3.36 3.21 1.90 2.41 1.95 hsa-asg-8329hsa-asg-8329_st1 9.23 6.26 6.52 4.42 5.87 6.14 6.17 hsa-asg-6890hsa-asg-6890_st1 7.86 4.41 4.47 4.01 4.59 3.85 4.32 hsa-miR-154*hsa-miR-154-_st2 7.89 5.81 3.48 4.21 4.98 4.59 3.89 hsa-asg-1270hsa-asg-1270_st2 7.19 4.22 3.58 1.89 4.06 4.54 4.83 hsa-miR-487ahsa-asg-6915_st1 7.28 4.07 3.58 3.90 3.38 3.85 3.72 hsa-asg-12531hsa-asg-12531_st1 6.76 3.18 3.58 1.63 3.19 3.45 4.38 hsa-miR-526bhsa-miR-526b_st2 6.07 2.22 2.67 3.21 3.05 1.80 2.57 hsa-miR-767-5phsa-asg-558_st1 5.47 3.05 2.32 0.88 1.79 0.71 1.57 hsa-asg-5741_st2hsa-asg-5741_st2 7.21 4.00 5.14 2.63 4.38 4.64 2.73 hsa-asg-1196hsa-asg-1196_st1 10.73 8.26 7.60 5.88 6.63 8.41 8.06 hsa-asg-7388hsa-asg-7388_st1 9.76 6.51 6.92 5.47 6.19 6.95 6.91 hsa-asg-2845hsa-asg-2845_st2 6.45 2.90 3.78 3.04 3.66 1.98 3.14 hsa-asg-4472hsa-asg-4472_st2 6.67 3.73 3.95 2.84 2.90 3.59 4.29 hsa-asg-3322hsa-asg-3322_st2 6.53 3.53 4.85 1.89 1.28 3.45 3.89 hsa-asg-12253hsa-asg-12253_st2 9.05 4.93 7.02 4.90 4.98 5.87 7.17 hsa-asg-8293hsa-asg-8293_st2 9.92 7.00 7.28 4.93 6.25 7.31 7.08 hsa-miR-542-5phsa-miR-542-5p_st2 7.25 3.91 2.83 3.66 3.60 3.81 2.80 hsa-asg-3185hsa-asg-3185_st1 7.17 4.47 4.21 2.74 4.92 4.49 5.21 hsa-asg-3406hsa-asg-3406_st1 6.83 3.73 3.05 3.13 2.98 3.81 4.08 hsa-asg-12515hsa-asg-12515_st2 7.36 4.00 4.25 3.04 4.10 4.57 4.32 hsa-cand330hsa-cand330_st2 7.57 4.74 4.98 3.52 4.70 5.12 4.12 hsa-asg-9491hsa-asg-9491_st1 7.92 4.53 4.96 3.72 4.82 4.69 4.96 hsa-asg-8039hsa-asg-8039_st2 6.96 3.31 4.28 3.13 3.44 2.79 3.43 hsa-asg-3731hsa-asg-3731_st1 8.37 4.59 6.57 4.46 4.65 4.59 5.77 hsa-miR-449bhsa-miR-449b_st1 6.53 1.83 3.24 4.46 2.48 3.55 2.80 hsa-miR-654hsa-miR-654_st1 7.24 2.41 5.19 4.16 3.89 3.81 5.45 hsa-miR-654hsa-asg-33_st1 7.47 3.18 6.20 5.09 4.51 4.41 5.15 hsa-asg-12959hsa-asg-12959_st1 6.52 3.91 4.31 2.84 3.38 2.57 3.97 hsa-miR-525hsa-miR-525_st2 5.30 2.41 1.54 2.63 0.57 2.57 2.14 hsa-asg-5205hsa-asg-5205_st1 10.35 8.01 7.74 5.04 7.49 8.27 6.87 hsa-asg-12440hsa-asg-12440_st2 8.00 4.74 4.83 3.84 4.75 4.80 5.23 hsa-asg-1319hsa-asg-1319_st2 9.04 5.50 6.23 5.26 5.74 6.43 6.09 hsa-asg-7935hsa-asg-7935_st2 9.07 6.98 6.67 4.30 6.23 6.47 5.80 hsa-asg-8896hsa-asg-8896_st2 8.07 3.73 5.27 4.16 5.11 4.84 4.52 hsa-asg-10236hsa-asg-10236_st2 7.08 4.07 3.48 3.04 4.51 5.14 3.72 hsa-asg-8668hsa-asg-8668_st2 7.11 4.53 4.11 3.21 5.05 5.03 4.49 hsa-asg-9849hsa-asg-9849_st2 9.95 6.37 7.98 5.47 6.39 6.76 7.48 hsa-asg-13471hsa-asg-13471_st1 7.88 4.74 4.71 4.38 5.11 5.31 5.34 hsa-asg-9334hsa-asg-9334_st2 9.96 6.32 7.60 6.15 6.41 6.92 7.31 hsa-miR-124ahsa-miR-124a_st1 7.02 4.29 4.18 3.78 4.80 3.81 3.32 hsa-miR-124ahsa-cand79_st1 6.58 3.53 3.78 3.78 4.44 3.64 2.14 hsa-asg-5265hsa-asg-5265_st1 9.73 6.74 7.06 5.82 6.30 7.25 6.97 hsa-asg-381hsa-asg-381_st1 6.49 3.05 3.58 3.04 3.66 3.81 3.32 hsa-asg-11698hsa-asg-11698_st2 11.15 8.56 9.32 6.82 7.99 8.60 9.03 hsa-asg-8638hsa-asg-8638_st1 10.34 7.94 7.93 5.88 7.27 8.03 7.81 hsa-miR-517chsa-miR-517c_st2 5.82 2.90 2.50 3.04 3.60 3.06 3.43 hsa-miR-542-3phsa-miR-542-3p_st1 8.65 5.09 5.25 6.01 6.29 5.71 5.62 hsa-asg-11443hsa-asg-11443_st1 6.18 4.15 3.58 2.84 2.66 3.50 2.65 hsa-miR-512-3phsa-miR-512-3p_st2 5.93 2.58 2.67 4.01 2.10 2.57 1.95 hsa-asg-265hsa-asg-265_st2 9.05 6.45 7.13 5.14 5.70 5.87 7.03 hsa-asg-1310hsa-asg-1310_st1 5.30 2.90 2.32 1.63 2.10 2.93 3.53 hsa-asg-508hsa-asg-508_st2 9.48 6.70 7.04 5.14 6.71 7.24 7.14 hsa-asg-8902hsa-asg-8902_st2 7.73 6.29 5.73 4.25 4.25 5.09 5.04 hsa-asg-8476hsa-asg-8476_st1 9.02 5.58 6.36 6.43 6.32 5.75 6.17 hsa-asg-11523hsa-asg-11523_st1 11.01 8.99 8.63 7.47 8.25 8.86 8.36 hsa-asg-1341hsa-asg-1341_st1 12.79 10.8 10.54 8.34 10.2 10.8 10.19 hsa-asg-8763hsa-asg-8763_st2 9.28 7.01 7.42 5.12 6.57 6.75 7.04 hsa-asg-4840hsa-asg-4840_st1 7.74 5.44 5.02 3.52 5.28 5.40 4.98 hsa-asg-12298hsa-asg-12298_st1 6.96 4.64 5.08 4.06 3.89 3.81 4.15 hsa-asg-6934hsa-asg-6934_st2 6.07 2.75 4.50 2.40 2.57 2.72 3.97 hsa-asg-9515hsa-asg-9515_st1 8.44 5.05 5.88 5.09 5.36 5.72 6.20 hsa-asg-2535hsa-asg-2535_st2 9.91 8.09 7.44 5.53 7.17 7.75 7.13 hsa-asg-5272hsa-asg-5272_st2 8.29 5.88 6.40 4.16 5.36 5.87 6.21 hsa-miR-507hsa-miR-507_st1 4.91 1.83 2.13 2.84 1.28 1.98 2.14 hsa-miR112hsa-miR112_st1 10.27 7.55 8.23 6.14 7.20 7.94 8.13 hsa-asg-8255hsa-asg-8255_st2 8.93 6.15 7.00 4.90 6.43 6.71 6.76 hsa-miR-369-3phsa-miR-369-3p_st1 5.42 2.58 1.74 2.63 2.90 3.40 1.95 hsa-asg-8446hsa-asg-8446_st2 8.43 5.24 6.40 4.46 5.31 6.41 6.13 hsa-asg-4654hsa-asg-4654_st1 11.29 9.21 9.10 7.53 8.49 9.12 9.20 hsa-miR-129hsa-miR-129_st1 7.45 4.84 4.76 5.24 4.80 4.17 3.89 hsa-cand805hsa-cand805_st2 11.02 8.23 9.20 7.34 8.24 8.82 8.70 hsa-asg-11237hsa-asg-11237_st1 12.44 10.7 9.91 8.46 10.2 10.9 9.77 hsa-asg-3728hsa-asg-3728_st1 9.16 6.64 7.33 5.43 6.53 6.79 6.90 hsa-asg-758hsa-asg-758_st1 8.89 6.45 6.59 5.07 6.13 7.04 6.24 hsa-asg-9269hsa-asg-9269_st2 5.82 2.58 2.50 4.68 2.90 2.57 2.65 hsa-miR-496hsa-miR-496_st1 4.94 2.41 2.67 1.89 2.30 1.62 2.49 hsa-asg-7130hsa-asg-7130_st1 8.20 4.93 6.25 4.65 5.34 5.51 6.13 hsa-asg-2844hsa-asg-2844_st1 5.67 3.73 4.18 2.15 2.82 1.98 2.94 hsa-asg-13231hsa-asg-13231_st1 6.28 3.91 4.11 4.21 4.44 2.93 3.38 hsa-asg-14185hsa-asg-14185_st1 7.32 3.64 5.35 3.38 4.67 4.59 5.58 hsa-asg-11802hsa-asg-11802_st1 9.78 7.71 8.25 6.01 7.20 7.73 7.92 hsa-miR-132hsa-miR-132_st1 11.10 9.71 8.51 8.34 8.34 8.56 8.02 hsa-asg-9500hsa-asg-9500_st1 6.70 4.59 4.58 3.59 3.80 3.55 4.08 hsa-asg-4789hsa-asg-4789_st1 7.18 4.41 5.19 3.30 4.21 3.89 5.61 hsa-cand287hsa-cand287_st1 5.60 2.22 2.97 2.63 3.38 3.12 2.94 hsa-asg-4392hsa-asg-4392_st1 12.36 10.6 10.03 8.15 10.0 10.8 9.84 hsa-asg-4438hsa-asg-4438_st2 7.36 4.53 4.50 5.04 4.53 4.11 4.49 hsa-asg-9513hsa-asg-9513_st1 8.87 5.93 6.75 5.33 5.83 6.13 6.33 hsa-asg-6292hsa-asg-6292_st2 11.48 9.76 9.57 7.45 8.84 9.88 8.81 hsa-asg-7376hsa-asg-7376_st1 5.32 3.05 2.13 2.15 2.39 2.41 3.53 hsa-asg-7048hsa-asg-7048_st1 7.50 5.05 5.75 3.59 4.92 4.20 5.40 hsa-asg-1239hsa-asg-1239_st1 8.26 5.13 6.38 4.25 5.24 5.98 6.69 hsa-asg-924hsa-asg-924_st1 6.11 4.64 4.25 3.38 4.44 2.65 2.73 hsa-asg-12381hsa-asg-12381_st1 8.91 6.03 7.14 5.01 5.83 6.71 6.84 hsa-asg-9920hsa-asg-9920_st1 10.01 8.07 8.33 6.09 7.77 8.07 7.77 hsa-cand207hsa-cand207_st1 5.88 2.58 4.11 3.66 2.48 3.64 3.63 hsa-cand266hsa-cand266_st1 9.07 6.73 7.20 5.49 7.08 6.90 7.03 hsa-asg-4915hsa-asg-4915_st1 5.65 2.41 3.78 2.15 2.57 3.59 2.87 hsa-cand899hsa-cand899_st2 6.71 2.75 4.74 3.30 3.98 4.17 4.77 hsa-asg-9727hsa-asg-9727_st1 7.04 4.64 5.64 3.52 4.38 5.03 4.70 hsa-asg-11850hsa-asg-11850_st1 12.54 10.5 10.17 8.54 10.4 11.1 9.97 hsa-asg-14230hsa-asg-14230_st1 6.48 4.29 3.68 4.38 4.56 3.29 3.43 hsa-asg-8303hsa-asg-8303_st2 6.19 3.31 4.50 4.30 4.53 4.04 4.12 hsa-asg-7999hsa-asg-7999_st1 5.78 3.73 3.95 3.21 3.12 1.98 3.32 hsa-miR144hsa-miR144_st2 6.01 4.59 4.38 2.63 3.38 2.65 2.65 hsa-cand764hsa-cand764_st1 8.46 5.86 6.04 5.55 5.73 6.35 6.04 hsa-asg-7250hsa-asg-7250_st1 6.39 4.00 4.83 2.52 3.32 4.59 3.97 hsa-asg-4808hsa-asg-4808_st1 7.13 3.73 6.08 3.84 3.71 4.36 5.61 hsa-asg-7475hsa-asg-7475_st1 6.64 3.53 4.11 3.78 4.21 4.57 3.72 hsa-asg-6063hsa-asg-6063_st2 11.36 9.14 9.37 8.01 9.04 9.46 9.16 hsa-asg-4759hsa-asg-4759_st1 9.42 7.03 7.97 5.85 6.74 7.35 7.54 hsa-miR-520dhsa-miR-520d_st1 5.58 2.75 3.36 2.15 2.90 3.50 3.58 hsa-asg-887hsa-asg-887_st2 8.81 6.39 7.06 5.62 6.29 6.81 7.01 hsa-asg-13047hsa-asg-13047_st2 7.69 5.31 5.96 4.71 5.52 4.41 5.71 hsa-asg-9687hsa-asg-9687_st1 6.72 4.74 4.76 3.90 4.65 3.85 4.19 hsa-asg-9509hsa-asg-9509_st2 8.07 4.47 6.82 4.65 5.09 5.37 6.38 hsa-asg-3373hsa-asg-3373_st2 5.32 3.53 1.94 2.63 3.75 4.04 3.63 hsa-asg-5889hsa-asg-5889_st2 5.96 3.73 4.31 3.78 3.32 3.97 4.32 hsa-asg-11565hsa-asg-11565_st1 6.40 4.00 4.94 3.04 3.12 3.93 4.79 hsa-asg-13013hsa-asg-13013_st1 4.97 2.75 2.67 2.63 2.10 2.33 1.76 hsa-asg-2041hsa-asg-2041_st1 8.08 5.93 6.09 5.37 5.59 5.87 5.70 hsa-asg-1169hsa-asg-1169_st2 7.92 5.27 6.60 4.34 5.38 5.59 6.19 hsa-miR-217hsa-miR-217_st1 5.52 3.31 3.58 2.63 2.48 3.77 3.32 hsa-asg-7460hsa-asg-7460_st1 5.96 2.41 4.71 3.38 3.19 3.45 4.79 hsa-asg-9681hsa-asg-9681_st1 6.58 4.84 4.25 4.99 4.75 3.68 2.80 hsa-asg-1293hsa-asg-1293_st2 8.48 6.39 6.83 5.59 6.19 6.21 6.58 hsa-asg-202hsa-asg-202_st1 6.68 4.29 5.60 3.13 3.75 4.27 5.11 hsa-miR-522hsa-miR-522_st2 5.47 2.75 2.83 3.66 3.12 2.93 3.43 hsa-asg-2074hsa-asg-2074_st2 5.47 2.41 3.95 2.40 3.25 3.45 3.53 hsa-asg-3614hsa-asg-3614_st1 6.90 4.79 5.36 3.59 4.38 4.00 5.11 hsa-asg-14176hsa-asg-14176_st1 7.87 6.05 5.67 5.28 6.77 6.23 5.37 hsa-asg-11977hsa-asg-11977_st1 8.29 6.15 6.74 4.90 5.92 6.33 7.12 hsa-asg-6234hsa-asg-6234_st1 12.09 10.4 10.68 8.61 10.0 10.3 10.42 hsa-asg-14006hsa-asg-14006_st2 7.15 4.74 5.35 4.75 4.96 4.90 4.15 hsa-cand659hsa-cand659_st1 10.14 7.82 7.93 7.34 7.90 8.06 8.08 hsa-asg-4069hsa-asg-4069_st1 6.48 4.29 4.88 2.74 4.44 4.27 4.92 hsa-asg-9209hsa-asg-9209_st2 8.73 6.13 6.97 5.97 6.34 6.48 6.84 hsa-asg-8557hsa-asg-8557_st1 7.80 6.05 5.87 4.90 5.15 5.72 5.61 hsa-asg-3783hsa-asg-3783_st1 8.08 5.55 6.98 4.42 5.24 6.05 6.81 hsa-miR-499hsa-miR12_st1 7.21 5.44 4.76 5.37 6.66 4.07 4.38 hsa-asg-9196hsa-asg-9196_st2 5.60 2.41 4.71 3.21 3.55 2.41 4.12 hsa-asg-4207hsa-asg-4207_st1 7.10 5.09 5.41 4.01 4.82 5.62 5.57 hsa-asg-11847hsa-asg-11847_st1 8.86 7.23 7.50 5.47 6.45 7.02 6.96 hsa-asg-7140hsa-asg-7140_st2 8.34 6.51 6.31 6.09 5.07 6.48 6.24 hsa-asg-1934hsa-asg-1934_st1 5.19 3.31 3.58 2.15 1.48 3.50 3.20 hsa-asg-6282hsa-asg-6282_st1 8.32 6.68 6.71 4.58 6.04 6.63 6.94 hsa-miR-370hsa-miR-370_st1 7.13 4.64 5.23 4.71 5.05 4.69 5.34 hsa-asg-8636hsa-asg-8636_st2 8.43 5.84 6.86 5.43 5.53 6.21 6.65 hsa-asg-9143hsa-asg-9143_st2 9.15 7.30 7.88 5.81 6.70 7.21 7.66 hsa-asg-8280hsa-asg-8280_st2 7.46 6.31 4.98 3.90 5.09 6.20 4.60 hsa-asg-11992hsa-asg-11992_st2 9.26 7.35 7.73 5.12 6.45 7.50 7.48 hsa-asg-2311hsa-asg-2311_st1 6.93 4.84 5.68 4.65 4.38 4.59 5.18 hsa-asg-7170hsa-asg-7170_st2 6.16 4.22 3.78 3.21 4.10 3.81 4.70 hsa-asg-3168hsa-asg-3168_st1 5.37 3.73 3.78 3.38 1.48 2.65 4.19 hsa-asg-8404hsa-asg-8404_st2 5.84 2.22 4.25 3.04 3.55 4.17 3.63 hsa-miR-212hsa-miR-212_st2 7.60 6.26 5.68 6.18 5.71 5.14 5.05 hsa-asg-10500hsa-asg-10500_st2 6.11 3.73 4.85 2.40 3.38 3.59 4.05 hsa-asg-1174hsa-asg-1174_st2 9.38 7.36 8.00 6.23 7.22 7.48 7.71 hsa-miR-9*hsa-miR-9-_st2 7.76 6.19 6.05 5.57 6.10 5.37 5.81 hsa-cand705hsa-cand705_st2 4.84 2.58 2.97 2.63 2.48 2.33 2.80 hsa-asg-3376hsa-asg-3376_st1 6.01 3.83 2.97 4.87 4.65 3.97 4.12 hsa-asg-1966hsa-asg-1966_st2 6.67 4.74 4.11 5.28 4.41 5.39 4.32 hsa-asg-1621hsa-asg-1621_st2 8.64 6.07 7.40 6.25 6.14 6.45 7.18 hsa-asg-7465hsa-asg-7465_st2 7.01 4.64 4.31 4.01 6.17 5.05 4.15 hsa-asg-14066hsa-asg-14066_st2 8.69 7.21 7.18 5.79 6.81 6.59 6.96 hsa-asg-10883hsa-asg-10883_st1 9.24 7.63 7.13 7.34 8.22 8.00 6.52 hsa-asg-4439hsa-asg-4439_st2 5.42 3.83 4.31 3.21 2.66 1.62 3.63 hsa-miR166hsa-miR166_st1 7.02 4.93 5.94 4.50 5.22 5.00 5.64 hsa-miR219hsa-miR219_st2 6.42 4.69 5.52 3.13 3.94 4.27 5.11 hsa-asg-1168hsa-asg-1168_st1 8.10 6.15 6.80 5.26 5.78 6.54 6.64 hsa-asg-4597hsa-asg-4597_st1 9.59 7.93 8.59 6.94 7.28 7.74 8.38 hsa-miR-518c*hsa-miR-518c-_st2 6.12 3.83 5.14 4.38 3.12 3.35 4.38 hsa-miR-372hsa-miR-372_st2 6.03 4.64 3.36 4.11 3.71 4.24 3.97 hsa-asg-1919hsa-asg-1919_st1 9.25 7.21 7.70 5.94 7.29 7.74 7.80 hsa-asg-4070hsa-asg-4070_st2 8.72 6.64 7.70 5.21 6.64 7.43 7.68 hsa-asg-3746hsa-asg-3746_st1 9.70 8.01 8.78 6.56 7.51 8.00 8.44 hsa-asg-4155hsa-asg-4155_st1 7.84 5.88 6.64 4.75 5.65 6.25 6.50 hsa-asg-9257hsa-asg-9257_st2 7.71 4.69 6.40 4.68 5.55 5.67 6.39 hsa-miR-371hsa-miR-371_st2 5.71 3.91 4.18 3.78 3.85 3.40 3.48 hsa-asg-11883hsa-asg-11883_st1 8.09 5.90 6.01 6.68 6.73 6.15 5.96 hsa-asg-10414hsa-asg-10414_st1 9.30 6.96 8.43 5.97 7.02 7.67 7.99 hsa-asg-13613hsa-asg-13613_st2 9.73 8.18 8.61 6.70 7.93 8.46 8.36 hsa-asg-8597hsa-asg-8597_st1 7.49 4.93 5.00 5.69 5.46 5.85 5.56 hsa-asg-9248hsa-asg-9248_st2 10.14 8.57 8.95 7.30 8.04 8.83 8.94 hsa-asg-2315hsa-asg-2315_st2 6.80 5.58 5.78 4.58 4.65 5.36 4.85 hsa-asg-7043hsa-asg-7043_st2 6.80 4.35 6.00 3.84 4.14 5.03 5.71 hsa-asg-8956hsa-asg-8956_st1 8.07 6.34 7.08 4.84 6.32 6.43 6.92 hsa-asg-7764hsa-asg-7764_st2 10.24 8.66 9.01 7.07 8.30 8.94 8.99 hsa-asg-10638hsa-asg-10638_st2 10.66 9.24 9.20 7.43 8.68 9.23 9.03 hsa-miR-7hsa-miR-7_st1 10.56 9.45 8.18 9.71 8.61 8.16 7.65 hsa-asg-3291hsa-asg-3291_st2 8.24 6.55 6.77 6.18 5.63 6.74 6.01 hsa-asg-1860hsa-asg-1860_st1 10.79 9.32 9.61 7.85 8.80 9.84 9.38 hsa-asg-6813hsa-asg-6813_st2 6.35 4.41 5.72 4.11 3.60 5.02 5.25 hsa-asg-11619hsa-asg-11619_st1 8.82 6.41 7.82 6.44 6.54 7.33 7.73 hsa-asg-13052hsa-asg-13052_st1 5.54 3.83 4.11 2.40 3.71 3.64 4.32 hsa-asg-1462hsa-asg-1462_st2 5.96 4.00 4.25 3.90 4.28 4.27 4.19 hsa-asg-5326hsa-asg-5326_st2 8.20 6.95 7.13 5.74 6.26 6.65 6.47 hsa-miR-660hsa-miR-660_st1 10.42 9.28 8.94 7.94 9.15 8.60 8.38 hsa-miR-373hsa-miR-373_st1 5.93 4.00 4.11 4.21 4.62 4.17 3.97 hsa-asg-7690hsa-asg-7690_st1 6.04 5.24 4.44 3.78 4.53 4.49 4.12 hsa-asg-2049hsa-asg-2049_st2 5.07 3.83 3.36 2.40 3.71 3.24 3.43 hsa-asg-2290hsa-asg-2290_st2 9.74 8.30 8.56 7.13 7.79 8.20 8.71 hsa-asg-7240hsa-asg-7240_st1 7.62 6.05 6.19 5.01 6.10 6.23 6.05 hsa-asg-12964hsa-asg-12964_st2 5.63 4.88 2.83 4.01 4.25 3.89 2.65 hsa-asg-13780hsa-asg-13780_st1 5.04 2.03 3.86 3.38 3.12 2.93 2.65 hsa-miR-9hsa-miR-9_st2 5.16 3.42 3.48 2.84 3.25 3.59 2.14 hsa-cand208hsa-cand208_st1 4.87 2.58 3.48 3.04 3.12 3.29 3.53 hsa-asg-3073hsa-asg-3073_st2 7.35 5.37 6.09 4.99 4.96 5.74 6.15 hsa-asg-11155hsa-asg-11155_st1 9.30 8.13 8.35 6.47 7.60 8.03 8.06 hsa-asg-1617hsa-asg-1617_st2 8.78 6.64 7.69 5.82 6.99 7.43 7.95 hsa-asg-12069hsa-asg-12069_st1 8.70 6.19 7.85 6.05 6.47 6.96 7.76 hsa-miR-501hsa-miR-501_st1 6.21 5.24 4.81 2.84 5.09 4.04 4.60 hsa-asg-5413hsa-asg-5413_st2 7.45 5.44 6.76 5.33 5.62 6.37 6.00 hsa-asg-8545hsa-asg-8545_st1 6.62 5.09 5.84 4.54 3.89 5.03 5.77 hsa-asg-92hsa-asg-92_st1 6.25 4.15 5.53 4.01 4.14 4.24 5.15 hsa-asg-14155hsa-asg-14155_st2 5.45 4.22 3.24 4.68 3.44 3.40 3.26 hsa-asg-1638hsa-asg-1638_st2 5.35 3.31 3.11 3.66 4.44 3.97 3.81 hsa-asg-9666hsa-asg-9666_st2 6.37 4.93 5.54 3.72 4.48 5.03 4.85 hsa-asg-2996hsa-asg-2996_st2 9.84 8.11 8.57 7.47 8.20 8.85 8.57 hsa-miR-215hsa-miR-215_st1 7.42 6.95 5.77 5.71 6.66 5.51 4.79 hsa-asg-12874hsa-asg-12874_st1 6.15 4.00 4.18 4.01 4.85 4.57 4.83 hsa-asg-13754hsa-asg-13754_st2 5.49 3.42 4.38 3.04 4.14 2.86 4.38 hsa-asg-7681hsa-asg-7681_st2 6.56 4.22 5.84 4.30 4.38 4.71 5.79 hsa-asg-4027hsa-asg-4027_st2 9.53 7.90 8.48 7.13 7.76 8.27 8.47 hsa-miR102hsa-miR102_st2 10.06 8.89 8.26 8.71 8.69 8.11 7.77 hsa-miR-128ahsa-miR-128a_st2 11.03 10.7 9.31 9.72 9.95 8.83 8.94 hsa-asg-8682hsa-asg-8682_st2 6.16 4.22 4.61 5.09 4.80 4.11 4.79 hsa-asg-954hsa-asg-954_st1 10.05 8.98 8.76 8.10 8.96 8.76 8.38 hsa-asg-1398hsa-asg-1398_st2 6.87 5.31 5.53 5.33 5.41 4.49 4.85 hsa-asg-1923hsa-asg-1923_st1 7.81 6.15 6.54 6.39 6.11 6.58 6.29 hsa-asg-9164hsa-asg-9164_st1 8.84 6.95 7.88 6.00 7.03 7.70 8.17 hsa-asg-7471hsa-asg-7471_st1 7.29 5.58 5.67 5.68 6.74 5.67 5.25 hsa-asg-748hsa-asg-748_st1 6.32 5.01 5.97 3.90 3.94 5.11 5.00 hsa-asg-9320hsa-asg-9320_st2 6.42 4.41 5.88 4.38 4.28 3.97 5.21 hsa-asg-8641hsa-asg-8641_st1 7.15 5.01 6.42 4.61 5.28 5.70 6.56 hsa-asg-6924hsa-asg-6924_st1 8.16 6.53 7.33 6.10 7.10 6.12 6.92 hsa-asg-4100hsa-asg-4100_st1 6.63 4.74 5.25 5.14 4.62 5.21 5.57 hsa-asg-1115hsa-asg-1115_st1 6.88 5.47 4.81 5.57 5.92 5.59 5.20 hsa-asg-4637hsa-asg-4637_st1 8.00 7.08 6.44 6.38 6.52 6.47 6.47 hsa-asg-7278hsa-asg-7278_st1 6.59 5.20 5.73 4.34 4.92 5.28 5.54 hsa-asg-4031hsa-asg-4031_st2 9.55 8.14 8.64 7.15 7.73 8.26 8.27 hsa-asg-8508hsa-asg-8508_st2 6.18 4.47 5.59 4.30 4.02 4.17 5.04 hsa-asg-3587hsa-asg-3587_st1 6.48 5.24 5.63 4.25 5.33 5.58 5.02 hsa-miR-500hsa-miR-500_st1 9.95 8.74 8.38 8.78 8.81 8.16 7.79 hsa-asg-11402hsa-asg-11402_st1 7.28 5.93 5.79 5.37 6.32 5.40 5.94 hsa-asg-13027hsa-asg-13027_st2 4.94 3.42 3.48 3.52 2.82 3.68 4.19 hsa-miR-376a*hsa-miR-376a-_st2 5.98 4.79 4.11 5.33 5.11 4.49 4.38 hsa-asg-7459hsa-asg-7459_st2 7.04 5.13 6.24 4.54 5.67 5.44 5.90 hsa-asg-2542hsa-asg-2542_st1 6.35 4.69 4.83 3.72 4.62 5.16 5.21 hsa-asg-5740hsa-asg-5740_st2 6.58 5.71 5.74 4.81 5.31 5.50 5.11 hsa-asg-9885hsa-asg-9885_st1 7.13 5.95 6.44 4.99 5.94 5.94 6.36 hsa-miR-566hsa-miR-566_st1 7.30 6.72 6.12 5.12 5.83 6.11 6.10 hsa-asg-6250hsa-asg-6250_st2 6.88 5.09 5.96 4.71 5.49 5.47 5.82 hsa-asg-5827hsa-asg-5827_st2 6.61 5.61 4.71 5.71 5.52 5.65 5.15 hsa-miR197hsa-miR197_st2 5.52 4.35 4.38 4.11 4.06 4.14 4.38 hsa-asg-10335hsa-asg-10335_st2 6.47 5.17 5.49 5.12 5.29 5.00 5.20 hsa-asg-11397hsa-asg-11397_st1 5.04 3.64 4.25 3.90 3.50 3.93 3.63 hsa-asg-11900hsa-asg-11900_st1 7.02 5.34 6.05 5.37 5.50 6.18 5.07 hsa-asg-8392hsa-asg-8392_st2 6.57 4.74 6.31 4.81 4.92 5.30 5.52 hsa-asg-414hsa-asg-414_st1 7.57 6.27 6.27 6.66 6.15 6.01 6.82 hsa-asg-7215hsa-asg-7215_st2 6.58 5.31 6.09 4.81 5.78 5.12 5.71 hsa-asg-8344hsa-asg-8344_st2 5.67 4.69 4.31 3.66 5.26 4.59 4.70 hsa-asg-13135hsa-asg-13135_st1 6.32 5.61 5.61 5.24 4.89 4.76 4.70 hsa-asg-3826hsa-asg-3826_st2 5.16 4.74 3.78 3.90 3.71 4.20 4.32 hsa-asg-7104hsa-asg-7104_st2 5.69 4.07 4.11 5.14 4.65 4.44 4.60 hsa-asg-2584hsa-asg-2584_st1 5.10 4.59 3.48 3.66 3.71 4.47 3.81 hsa-asg-4246hsa-asg-4246_st2 6.00 4.59 5.41 4.75 4.87 5.17 4.92 hsa-asg-3969hsa-asg-3969_st1 7.30 5.88 6.75 5.57 5.85 6.35 6.73 hsa-miR-362hsa-miR-362_st1 8.39 7.42 7.66 7.94 7.60 7.44 7.08 hsa-asg-10626hsa-asg-10626_st2 5.89 5.13 5.14 4.61 5.36 4.96 4.25 hsa-asg-6186hsa-asg-6186_st2 6.64 6.27 5.69 5.60 5.55 5.65 5.72 hsa-asg-11729hsa-asg-11729_st2 9.13 8.50 8.40 8.13 7.84 8.47 7.83 hsa-miR-584hsa-miR-584_st2 6.87 5.74 6.05 6.24 6.23 5.72 5.72 LNneg MicroRNA 7 8 9M SD % ttest log2Diff Fold hsa-asg-5638 1.25 2.74 3.22 2.51 0.81 03.28E−06 −9.71 835 hsa-miR-513 0.50 5.13 5.51 4.94 1.85 33 3.04E−03−8.17 287 hsa-asg-5630 1.09 2.89 4.21 3.19 1.07 0 1.60E−04 −7.47 177hsa-asg-1088 3.27 1.46 2.51 1.93 0.68 0 6.94E−06 −7.41 170 hsa-miR-5140.93 2.41 2.07 1.75 0.53 0 1.14E−06 −7.25 152 hsa-asg-11338 3.15 4.125.44 4.38 0.81 11 3.20E−05 −7.14 141 hsa-miR-509 5.27 5.27 5.40 5.950.70 56 1.28E−05 −6.94 123 hsa-miR-493-5p 4.36 1.85 4.58 4.66 1.58 334.31E−03 −6.54 93.0 hsa-asg-6283 4.96 2.14 1.95 4.19 1.50 11 3.40E−03−6.48 89.5 hsa-asg-13404 2.14 1.09 2.29 2.82 1.29 0 1.60E−03 −6.35 81.7hsa-miR-506 6.36 6.33 6.42 6.17 0.37 100 2.44E−07 −6.27 77.1hsa-asg-14208 3.03 4.04 3.94 3.63 1.09 0 8.45E−04 −5.97 62.8 hsa-miR-3832.62 2.58 1.36 2.46 0.71 0 4.30E−05 −5.96 62.4 hsa-asg-10309 3.91 3.032.29 4.22 1.45 33 4.48E−03 −5.96 62.2 hsa-miR-411 3.38 2.23 2.07 3.831.27 11 2.22E−03 −5.94 61.5 hsa-miR-493-3p 1.96 1.56 1.71 1.40 0.73 06.16E−05 −5.85 57.5 hsa-miR-493-3p 4.31 3.46 4.08 3.41 0.95 0 4.16E−03−3.99 15.8 hsa-asg-7751 4.05 1.66 2.29 3.42 1.17 0 1.55E−03 −5.78 55.0hsa-miR-510 4.52 4.25 5.07 4.81 0.32 11 1.32E−07 −5.77 54.6 hsa-miR-1873.03 3.35 3.14 3.33 0.61 0 2.00E−05 −5.76 54.2 hsa-miR-508 5.41 6.116.53 6.17 0.43 100 1.38E−06 −5.73 53.1 hsa-asg-13922 4.05 2.23 1.36 3.871.66 0 1.19E−02 −5.69 51.5 hsa-miR-517a 4.18 3.71 1.83 4.06 1.30 03.26E−03 −5.66 50.6 hsa-miR-517a 4.05 3.88 2.51 4.25 1.23 22 2.82E−03−5.50 45.4 hsa-miR-299-5p 5.03 2.41 3.94 4.74 1.40 44 5.10E−03 −5.6449.7 hsa-miR202 4.25 2.41 3.06 3.45 0.88 0 2.93E−04 −5.63 49.4hsa-asg-9514 4.18 2.58 3.36 3.43 0.79 0 1.57E−04 −5.52 46.0 hsa-asg-95106.28 4.63 3.84 6.21 1.40 0 5.92E−03 −5.48 44.7 hsa-miR-487b 4.88 3.714.37 5.58 1.11 67 1.61E−03 −5.46 44.0 hsa-asg-5617 1.42 1.46 1.60 2.781.03 0 1.06E−03 −5.41 42.4 hsa-asg-9512 6.35 5.27 5.20 6.23 1.01 119.89E−04 −5.39 41.8 hsa-miR-539 1.96 2.58 1.36 2.52 0.63 0 4.25E−05−5.35 40.8 hsa-miR-539 3.48 1.66 2.07 2.19 0.63 0 5.86E−05 −5.10 34.4hsa-miR-432 3.48 4.25 2.71 4.80 1.13 22 2.07E−03 −5.32 40.0 hsa-asg-74723.57 0.92 0.93 1.97 0.85 0 3.51E−04 −5.29 39.2 hsa-miR-495 5.14 2.414.87 5.23 1.36 56 6.20E−03 −5.28 39.0 hsa-asg-4037 2.31 1.85 2.29 2.510.58 0 2.53E−05 −5.28 38.9 hsa-asg-3081 3.75 3.88 1.36 3.99 1.21 113.25E−03 −5.27 38.6 hsa-asg-8486 3.27 3.29 2.89 3.24 0.44 0 3.07E−06−5.26 38.4 hsa-miR-127 6.57 5.47 5.42 6.51 1.03 56 1.35E−03 −5.23 37.6hsa-asg-20 1.42 2.32 1.48 2.76 1.20 0 3.35E−03 −5.21 37.1 hsa-asg-37432.14 2.32 3.89 3.19 0.68 0 9.04E−05 −5.20 36.7 hsa-miR-154 3.15 1.283.94 3.47 1.10 0 2.12E−03 −5.19 36.4 hsa-asg-5021 4.57 3.03 2.07 4.631.58 22 1.45E−02 −5.18 36.2 hsa-miR-369-5p 2.90 2.58 3.50 3.42 0.81 03.04E−04 −5.14 35.2 hsa-miR-369-5p 3.38 2.74 3.50 3.36 0.56 0 3.82E−05−4.82 28.2 hsa-asg-13166 3.57 2.05 4.04 3.94 0.88 0 6.76E−04 −5.00 32.0hsa-asg-13801 1.42 2.32 1.25 2.67 0.95 0 1.09E−03 −5.00 32.0 hsa-miR-1841.60 3.80 4.13 4.10 1.09 11 2.49E−03 −4.99 31.8 hsa-cand443 4.84 3.804.29 4.59 0.68 33 1.21E−04 −4.95 30.9 hsa-miR-409-3p 3.95 2.58 3.73 3.751.06 0 2.23E−03 −4.92 30.2 hsa-miR-134 4.71 4.29 5.00 5.16 0.68 01.39E−04 −4.90 29.9 hsa-asg-13254 4.71 2.41 3.36 4.86 1.37 56 9.54E−03−4.90 29.9 hsa-miR-376a 2.47 3.80 3.73 4.34 1.02 0 1.89E−03 −4.88 29.5hsa-miR-376a 3.83 4.12 4.64 4.83 0.75 11 3.08E−04 −4.79 27.7hsa-miR-376a 2.90 4.04 1.83 3.57 0.86 0 1.15E−03 −4.49 22.5 hsa-asg-10205.90 5.18 4.90 5.92 1.24 67 5.70E−03 −4.87 29.3 hsa-miR-487a 0.93 2.232.89 2.50 0.73 0 2.43E−04 −4.83 28.4 hsa-miR-379 6.33 6.13 6.20 6.580.37 100 1.67E−06 −4.81 28.1 hsa-asg-3597 6.67 5.96 5.77 6.60 0.88 788.49E−04 −4.80 27.9 hsa-asg-8242 3.43 1.66 2.29 3.11 1.16 0 4.37E−03−4.79 27.7 hsa-asg-3009 3.48 4.41 4.17 4.75 0.69 0 1.75E−04 −4.76 27.1hsa-miR-204 5.17 5.40 5.76 6.18 1.00 44 1.98E−03 −4.75 26.9 hsa-miR-3813.48 1.28 3.22 3.08 0.91 0 1.19E−03 −4.73 26.5 hsa-miR-520a* 0.78 2.410.93 1.81 0.83 0 6.49E−04 −4.71 26.2 hsa-asg-1608 3.98 2.14 1.95 3.751.19 0 5.79E−03 −4.70 26.0 hsa-miR-368 5.62 3.97 5.07 6.25 1.40 671.31E−02 −4.69 25.9 hsa-miR-526a 3.03 2.23 1.83 2.77 0.90 0 1.18E−03−4.68 25.6 hsa-cand206 3.57 1.28 2.71 2.65 0.86 0 8.59E−04 −4.67 25.5hsa-asg-3742 3.57 4.19 3.62 4.03 0.55 0 4.22E−05 −4.64 24.9 hsa-miR-2023.66 5.46 4.82 4.84 0.57 0 6.06E−05 −4.62 24.6 hsa-miR-485-5p 3.48 1.853.50 3.08 0.68 0 1.98E−04 −4.62 24.6 hsa-miR-518f* 1.60 3.03 2.51 2.540.91 0 1.33E−03 −4.60 24.3 hsa-miR-494 5.71 5.84 6.58 6.68 0.64 1001.42E−04 −4.58 23.9 hsa-miR-494 5.38 5.77 6.87 6.45 0.55 89 1.14E−04−4.07 16.8 hsa-miR-526c 3.15 2.58 2.07 2.91 0.99 0 2.33E−03 −4.57 23.8hsa-asg-677 3.66 4.12 2.71 4.04 0.72 0 3.32E−04 −4.57 23.7 hsa-asg-59966.21 6.37 3.73 6.66 1.44 0 1.69E−02 −4.57 23.7 hsa-miR-520g 3.03 2.892.51 2.77 0.50 0 2.52E−05 −4.53 23.1 hsa-asg-4756 1.25 3.23 1.95 2.830.97 0 2.14E−03 −4.53 23.1 hsa-miR-382 6.27 5.91 5.79 6.70 0.61 781.12E−04 −4.53 23.0 hsa-miR-450 4.18 4.44 4.04 3.76 0.62 0 1.21E−04−4.51 22.8 hsa-miR-450 2.90 2.89 2.71 2.79 0.42 0 7.85E−06 −4.42 21.4hsa-asg-12356 6.82 7.01 6.01 7.43 1.05 78 3.91E−03 −4.44 21.8hsa-asg-8129 1.96 4.22 2.18 3.30 1.03 0 3.48E−03 −4.42 21.4 hsa-cand7331.09 3.35 2.98 3.94 1.71 11 4.13E−02 −4.39 21.0 hsa-asg-6619 3.48 2.321.95 2.75 1.26 0 1.07E−02 −4.38 20.9 hsa-asg-8128 2.14 0.84 2.18 2.800.95 0 2.39E−03 −4.38 20.8 hsa-asg-14209 4.84 4.80 4.82 4.43 0.83 01.03E−03 −4.38 20.8 hsa-asg-7295 2.77 2.74 1.83 2.43 0.57 0 9.48E−05−4.33 20.1 hsa-asg-10478 3.66 2.74 2.71 3.39 0.69 0 3.58E−04 −4.31 19.8hsa-asg-7527 4.42 4.01 4.25 4.21 0.80 0 9.32E−04 −4.28 19.4 hsa-miR-4490.93 1.66 4.70 2.70 1.37 0 1.84E−02 −4.26 19.1 hsa-miR-503 6.39 6.605.99 6.62 0.46 78 2.49E−05 −4.23 18.8 hsa-miR-503 6.85 6.87 6.74 6.870.30 100 1.88E−06 −3.87 14.6 hsa-cand91 4.22 4.61 2.71 4.24 1.11 06.99E−03 −4.22 18.7 hsa-miR-608 2.62 1.56 2.80 3.28 1.17 0 9.25E−03−4.21 18.5 hsa-miR-135b 3.27 2.23 2.89 2.57 0.55 0 9.18E−05 −4.18 18.2hsa-miR-135b 3.57 1.66 3.22 3.18 0.75 0 4.58E−03 −3.09 8.5 hsa-asg-137411.42 1.46 3.06 1.94 0.86 0 1.81E−03 −4.17 18.0 hsa-miR-105 2.47 2.232.71 1.99 0.71 0 5.39E−04 −4.16 17.8 hsa-miR-433 1.60 1.66 2.89 2.801.00 0 4.38E−03 −4.16 17.8 hsa-miR-377 0.78 2.74 2.07 2.32 0.71 05.65E−04 −4.12 17.3 hsa-miR-572 6.27 7.18 5.36 6.82 1.43 0 2.62E−02−4.11 17.3 hsa-asg-13077 4.52 3.88 3.94 4.76 0.95 44 3.49E−03 −4.11 17.3hsa-miR-516-5p 2.31 3.61 3.06 2.84 0.61 0 2.25E−04 −4.10 17.2hsa-miR-516-5p 2.77 2.58 2.71 2.94 0.98 0 4.62E−03 −4.01 16.1hsa-asg-9009 4.92 3.66 3.99 4.59 0.61 0 2.17E−04 −4.08 17.0 hsa-asg-37825.56 5.90 5.57 6.10 0.57 0 1.33E−04 −4.08 16.9 hsa-miR-409-5p 5.90 5.435.24 5.63 0.58 44 1.61E−04 −4.08 16.9 hsa-miR-424 5.80 5.70 7.21 5.690.98 89 4.34E−03 −4.06 16.7 hsa-asg-1089 2.62 1.46 1.60 2.25 0.68 04.60E−04 −4.06 16.7 hsa-miR-485-3p 3.57 2.74 3.06 3.24 0.66 0 3.91E−04−4.06 16.6 hsa-miR-485-3p 3.66 3.51 3.84 3.74 0.51 0 1.95E−04 −3.50 11.3hsa-miR-410 3.15 1.85 2.51 2.51 0.68 0 4.85E−04 −4.06 16.6 hsa-asg-51493.66 4.78 4.90 4.86 0.51 0 6.82E−05 −4.05 16.5 hsa-asg-3672 4.57 4.474.48 5.18 0.80 11 1.37E−03 −4.04 16.5 hsa-miR-519d 3.15 2.05 2.51 2.580.67 0 4.35E−04 −4.03 16.4 hsa-asg-1199 2.77 3.80 1.36 2.80 0.80 01.47E−03 −4.00 16.1 hsa-asg-1619 2.31 3.03 3.22 3.30 0.78 0 1.30E−03−3.99 15.9 hsa-miR-329 3.66 2.74 3.06 3.16 0.84 0 2.06E−03 −3.98 15.7hsa-miR-329 2.77 3.29 2.71 3.41 0.74 0 9.82E−04 −3.92 15.2 hsa-miR-3293.98 2.58 3.50 3.58 0.59 0 4.00E−04 −3.64 12.4 hsa-asg-4283 3.48 2.232.51 3.19 0.65 0 4.30E−04 −3.95 15.4 hsa-cand930 2.47 4.08 4.25 3.530.89 0 2.96E−03 −3.95 15.4 hsa-asg-1881 3.48 4.22 4.08 3.98 0.92 03.70E−03 −3.95 15.4 hsa-miR-520h 2.47 2.05 2.71 2.63 0.45 0 3.22E−05−3.92 15.1 hsa-miR-34c 3.48 3.71 6.83 3.57 1.38 11 2.74E−02 −3.91 15.0hsa-miR-376b 2.77 2.50 0.22 2.28 1.17 0 1.30E−02 −3.91 15.0 hsa-asg-96966.61 6.53 5.07 6.46 0.65 89 4.68E−04 −3.90 15.0 hsa-asg-13878 3.03 2.411.83 3.21 0.90 0 3.54E−03 −3.89 14.8 hsa-asg-3754 4.84 5.50 4.61 4.810.57 0 1.99E−04 −3.84 14.4 hsa-miR-515-3p 0.78 1.85 1.83 1.51 0.50 08.70E−05 −3.81 14.1 hsa-miR-380-3p 1.96 0.76 1.83 1.57 0.67 0 6.49E−04−3.81 14.0 hsa-miR-380-3p 1.25 1.28 2.07 2.00 0.72 0 3.98E−03 −3.04 8.2hsa-cand561 2.77 3.84 3.99 3.05 0.96 0 5.45E−03 −3.81 14.0 hsa-asg-68154.25 5.40 5.20 5.25 0.74 22 1.19E−03 −3.81 14.0 hsa-asg-8616 3.83 2.053.06 3.41 1.02 0 7.63E−03 −3.79 13.9 hsa-asg-8630 3.57 4.91 5.62 5.321.14 22 1.34E−02 −3.79 13.8 hsa-cand150 2.62 3.97 4.44 3.60 1.21 111.88E−02 −3.76 13.5 hsa-asg-1203 3.91 3.71 1.83 3.70 0.90 0 4.24E−03−3.75 13.5 hsa-asg-7159 3.98 3.40 2.29 3.35 0.62 0 4.56E−04 −3.74 13.4hsa-asg-9262 3.75 4.08 4.48 4.46 0.58 0 2.71E−04 −3.74 13.4 hsa-asg-56061.60 2.66 2.80 2.65 0.78 0 1.96E−03 −3.73 13.3 hsa-asg-4872 2.62 3.883.94 3.56 0.68 0 8.78E−04 −3.69 12.9 hsa-asg-8893 4.71 3.46 4.33 4.700.55 0 2.31E−04 −3.67 12.8 hsa-asg-10947 3.57 2.58 2.51 3.32 0.98 07.40E−03 −3.67 12.7 hsa-asg-124 5.27 4.75 5.11 5.02 0.44 0 4.65E−05−3.64 12.5 hsa-asg-8987 1.96 3.84 4.79 3.58 1.12 0 1.51E−02 −3.64 12.5hsa-asg-3740 3.87 4.85 4.82 5.10 0.74 0 1.63E−03 −3.61 12.2 hsa-miR2235.67 5.28 5.09 5.38 0.47 0 8.79E−05 −3.61 12.2 hsa-asg-7671 5.60 6.175.30 6.26 0.67 78 9.66E−04 −3.60 12.1 hsa-asg-12735 6.36 6.06 5.30 6.440.63 78 6.45E−04 −3.60 12.1 hsa-asg-8138 4.80 5.15 5.30 5.63 0.79 442.62E−03 −3.60 12.1 hsa-miR-520f 2.31 2.41 3.06 2.16 0.49 0 1.18E−04−3.59 12.0 hsa-miR-299-3p 3.27 1.85 2.89 2.91 0.52 0 1.87E−04 −3.59 12.0hsa-miR-299-3p 1.60 3.17 3.50 2.75 0.78 0 2.59E−03 −3.55 11.7hsa-asg-8329 5.49 5.53 4.41 5.65 0.77 56 2.27E−03 −3.59 12.0hsa-asg-6890 4.15 4.25 4.44 4.28 0.24 0 5.55E−07 −3.58 12.0 hsa-miR-154*4.25 3.17 4.37 4.31 0.79 11 2.60E−03 −3.58 12.0 hsa-asg-1270 3.03 3.293.22 3.63 0.90 0 5.57E−03 −3.56 11.8 hsa-miR-487a 3.66 3.71 3.62 3.720.20 0 1.62E−07 −3.56 11.8 hsa-asg-12531 2.31 3.17 4.04 3.21 0.83 03.75E−03 −3.55 11.7 hsa-miR-526b 2.90 1.46 2.89 2.53 0.59 0 4.60E−04−3.54 11.7 hsa-miR-767-5p 2.31 2.05 2.71 1.93 0.78 0 2.64E−03 −3.54 11.6hsa-asg-5741_st2 4.18 2.05 3.50 3.69 1.04 0 1.24E−02 −3.51 11.4hsa-asg-1196 7.06 7.26 5.91 7.23 0.95 78 8.13E−03 −3.50 11.3hsa-asg-7388 5.90 5.83 5.80 6.28 0.56 67 3.75E−04 −3.48 11.2hsa-asg-2845 3.03 2.74 2.51 2.98 0.55 0 3.17E−04 −3.48 11.1 hsa-asg-44722.90 1.85 2.71 3.20 0.75 0 2.36E−03 −3.48 11.1 hsa-asg-3322 2.77 2.233.73 3.07 1.12 0 1.89E−02 −3.46 11.0 hsa-asg-12253 3.57 5.28 6.57 5.591.17 33 2.33E−02 −3.46 11.0 hsa-asg-8293 5.89 6.49 5.97 6.47 0.79 03.31E−03 −3.45 10.9 hsa-miR-542-5p 3.83 4.66 5.16 3.81 0.76 0 2.59E−03−3.44 10.9 hsa-asg-3185 2.31 2.50 3.14 3.78 1.11 0 1.97E−02 −3.40 10.5hsa-asg-3406 3.91 2.32 3.99 3.44 0.60 0 6.76E−04 −3.39 10.5hsa-asg-12515 3.66 4.12 3.73 3.98 0.45 0 9.72E−05 −3.38 10.4 hsa-cand3302.62 3.97 3.94 4.19 0.80 11 3.83E−03 −3.38 10.4 hsa-asg-9491 4.12 4.954.17 4.55 0.45 0 9.77E−05 −3.37 10.4 hsa-asg-8039 3.98 4.08 3.79 3.580.49 0 1.72E−04 −3.37 10.4 hsa-asg-3731 4.50 4.66 5.28 5.01 0.73 02.38E−03 −3.36 10.3 hsa-miR-449b 2.77 3.17 4.29 3.18 0.84 0 5.30E−03−3.36 10.2 hsa-miR-654 3.66 1.56 4.84 3.89 1.26 0 3.59E−02 −3.36 10.2hsa-miR-654 4.57 4.19 3.62 4.55 0.89 11 1.40E−02 −2.92 7.6 hsa-asg-129592.31 2.05 3.22 3.17 0.79 0 3.91E−03 −3.35 10.2 hsa-miR-525 2.77 1.091.83 1.95 0.76 0 3.01E−03 −3.35 10.2 hsa-asg-5205 6.68 7.58 5.36 7.011.14 44 2.40E−02 −3.34 10.2 hsa-asg-12440 4.42 4.78 4.54 4.66 0.38 03.11E−05 −3.34 10.1 hsa-asg-1319 5.38 5.87 5.09 5.73 0.46 11 1.32E−04−3.31 9.9 hsa-asg-7935 5.19 5.63 4.64 5.77 0.92 0 9.37E−03 −3.30 9.8hsa-asg-8896 5.36 4.95 5.09 4.78 0.54 0 4.26E−04 −3.29 9.8 hsa-asg-102363.15 3.71 3.36 3.80 0.68 0 1.77E−03 −3.28 9.7 hsa-asg-8668 3.57 2.412.07 3.83 1.09 0 2.13E−02 −3.28 9.7 hsa-asg-9849 6.11 6.55 7.02 6.680.74 89 3.14E−03 −3.27 9.6 hsa-asg-13471 4.36 4.04 3.50 4.61 0.61 119.57E−04 −3.27 9.6 hsa-asg-9334 6.22 6.60 6.85 6.71 0.50 89 2.76E−04−3.25 9.5 hsa-miR-124a 2.14 3.61 4.37 3.81 0.77 0 4.23E−03 −3.21 9.2hsa-miR-124a 2.31 3.40 4.82 3.54 0.87 0 1.07E−02 −3.04 8.2 hsa-asg-52656.30 6.40 5.96 6.53 0.50 89 2.91E−04 −3.20 9.2 hsa-asg-381 3.48 3.292.51 3.30 0.40 0 6.24E−05 −3.19 9.1 hsa-asg-11698 7.39 7.89 6.36 7.991.00 89 1.71E−02 −3.15 8.9 hsa-asg-8638 6.77 7.19 6.08 7.21 0.81 786.56E−03 −3.13 8.7 hsa-miR-517c 1.42 2.23 2.07 2.70 0.70 0 2.84E−03−3.13 8.7 hsa-miR-542-3p 5.38 5.88 4.70 5.55 0.49 78 3.42E−04 −3.10 8.6hsa-asg-11443 3.38 2.74 2.29 3.09 0.59 0 1.12E−03 −3.09 8.5hsa-miR-512-3p 3.15 2.41 4.13 2.84 0.78 0 5.42E−03 −3.09 8.5 hsa-asg-2655.27 5.77 5.36 5.97 0.74 44 4.21E−03 −3.08 8.4 hsa-asg-1310 0.93 1.662.29 2.25 0.79 0 6.67E−03 −3.04 8.2 hsa-asg-508 6.32 6.30 5.40 6.44 0.7478 4.70E−03 −3.04 8.2 hsa-asg-8902 4.52 4.22 3.29 4.74 0.90 22 1.37E−02−2.99 7.9 hsa-asg-8476 6.75 5.40 5.61 6.04 0.47 11 3.08E−04 −2.98 7.9hsa-asg-11523 7.18 7.76 6.81 8.04 0.77 100 6.33E−03 −2.97 7.8hsa-asg-1341 9.34 10.0 8.05 9.83 1.03 89 2.62E−02 −2.96 7.8 hsa-asg-87635.52 6.40 5.18 6.34 0.86 67 1.14E−02 −2.94 7.7 hsa-asg-4840 4.62 4.554.37 4.80 0.61 11 1.82E−03 −2.94 7.7 hsa-asg-12298 3.27 3.35 3.89 4.020.57 0 1.25E−03 −2.94 7.7 hsa-asg-6934 2.77 3.40 3.22 3.14 0.70 04.19E−03 −2.93 7.6 hsa-asg-9515 5.10 5.40 5.79 5.51 0.41 33 1.34E−04−2.93 7.6 hsa-asg-2535 6.37 7.85 5.64 7.00 0.94 33 1.90E−02 −2.92 7.5hsa-asg-5272 4.36 5.66 4.61 5.39 0.82 0 1.02E−02 −2.90 7.5 hsa-miR-5071.96 1.66 2.29 2.01 0.43 0 2.22E−04 −2.89 7.4 hsa-miR112 6.58 7.56 7.097.38 0.70 0 4.52E−03 −2.89 7.4 hsa-asg-8255 5.14 5.50 5.80 6.04 0.75 566.53E−03 −2.89 7.4 hsa-miR-369-3p 3.27 2.97 1.36 2.53 0.70 0 4.59E−03−2.89 7.4 hsa-asg-8446 4.71 5.70 5.51 5.54 0.70 33 4.30E−03 −2.89 7.4hsa-asg-4654 7.81 8.16 6.95 8.40 0.84 22 1.12E−02 −2.89 7.4 hsa-miR-1294.47 4.55 4.37 4.57 0.40 0 1.31E−04 −2.88 7.4 hsa-cand805 7.66 7.87 7.218.14 0.68 100 3.95E−03 −2.88 7.4 hsa-asg-11237 9.33 9.71 7.16 9.58 1.17100 4.81E−02 −2.86 7.3 hsa-asg-3728 5.52 5.81 5.68 6.29 0.69 56 4.37E−03−2.86 7.3 hsa-asg-758 5.41 6.52 4.79 6.03 0.76 67 7.41E−03 −2.86 7.3hsa-asg-9269 2.62 2.23 3.94 2.96 0.80 0 9.78E−03 −2.86 7.2 hsa-miR-4962.14 1.66 1.83 2.11 0.38 0 1.03E−04 −2.83 7.1 hsa-asg-7130 4.67 5.445.49 5.38 0.57 22 1.57E−03 −2.82 7.0 hsa-asg-2844 2.77 2.58 2.51 2.850.71 0 5.38E−03 −2.81 7.0 hsa-asg-13231 1.78 2.05 4.44 3.47 1.01 03.04E−02 −2.80 7.0 hsa-asg-14185 3.91 4.12 5.44 4.52 0.82 0 1.15E−02−2.80 7.0 hsa-asg-11802 5.85 6.37 5.84 6.99 0.97 0 2.56E−02 −2.80 6.9hsa-miR-132 8.00 8.42 6.86 8.31 0.74 89 7.08E−03 −2.79 6.9 hsa-asg-95003.15 4.15 3.68 3.91 0.48 0 5.88E−04 −2.79 6.9 hsa-asg-4789 4.05 4.014.84 4.39 0.71 0 6.01E−03 −2.79 6.9 hsa-cand287 2.14 3.51 2.51 2.83 0.480 6.15E−04 −2.78 6.9 hsa-asg-4392 9.19 10.0 7.54 9.60 1.11 100 4.67E−02−2.76 6.8 hsa-asg-4438 4.18 4.80 5.32 4.61 0.39 0 1.50E−04 −2.75 6.7hsa-asg-9513 5.74 6.45 6.64 6.13 0.46 78 5.06E−04 −2.75 6.7 hsa-asg-62927.97 9.26 7.09 8.74 1.02 67 3.39E−02 −2.75 6.7 hsa-asg-7376 2.14 2.413.06 2.59 0.50 0 8.74E−04 −2.74 6.7 hsa-asg-7048 4.31 4.01 5.62 4.760.77 11 9.63E−03 −2.73 6.6 hsa-asg-1239 4.52 5.55 6.04 5.53 0.82 331.37E−02 −2.73 6.6 hsa-asg-924 2.14 2.74 3.50 3.38 0.89 0 2.04E−02 −2.726.6 hsa-asg-12381 5.69 5.94 6.74 6.21 0.69 67 5.72E−03 −2.70 6.5hsa-asg-9920 6.61 6.76 6.38 7.32 0.85 44 1.66E−02 −2.70 6.5 hsa-cand2072.62 2.74 3.22 3.19 0.60 0 2.70E−03 −2.69 6.4 hsa-cand266 5.86 5.97 5.166.38 0.76 78 1.03E−02 −2.69 6.4 hsa-asg-4915 2.77 2.05 4.44 2.96 0.81 01.36E−02 −2.69 6.4 hsa-cand899 3.38 4.22 5.09 4.04 0.78 0 1.16E−02 −2.676.3 hsa-asg-9727 3.15 4.04 4.29 4.38 0.75 11 1.00E−02 −2.66 6.3hsa-asg-11850 9.57 10.4 8.00 9.88 1.02 100 3.87E−02 −2.66 6.3hsa-asg-14230 2.77 3.93 4.04 3.82 0.58 0 2.49E−03 −2.66 6.3 hsa-asg-83031.96 2.41 2.71 3.54 0.97 0 3.24E−02 −2.65 6.3 hsa-asg-7999 1.42 3.513.94 3.13 0.87 0 2.06E−02 −2.65 6.3 hsa-miR144 2.77 3.17 4.21 3.38 0.810 1.47E−02 −2.63 6.2 hsa-cand764 5.03 6.36 5.62 5.84 0.42 44 3.40E−04−2.62 6.2 hsa-asg-7250 3.15 4.41 3.14 3.77 0.78 0 1.29E−02 −2.62 6.1hsa-asg-4808 4.05 3.93 5.34 4.52 0.91 0 2.63E−02 −2.61 6.1 hsa-asg-74753.57 4.75 4.04 4.03 0.43 0 4.10E−04 −2.61 6.1 hsa-asg-6063 8.43 8.367.81 8.75 0.61 56 3.71E−03 −2.60 6.1 hsa-asg-4759 5.92 6.42 6.54 6.820.72 89 9.03E−03 −2.60 6.1 hsa-miR-520d 3.15 2.41 3.22 3.00 0.49 01.07E−03 −2.58 6.0 hsa-asg-887 5.10 5.87 5.91 6.23 0.67 56 6.29E−03−2.58 6.0 hsa-asg-13047 4.88 4.68 4.90 5.12 0.53 0 1.66E−03 −2.57 6.0hsa-asg-9687 4.05 3.71 3.50 4.15 0.47 0 8.10E−04 −2.57 6.0 hsa-asg-95095.10 6.10 5.52 5.50 0.79 33 1.49E−02 −2.57 6.0 hsa-asg-3373 1.78 1.851.83 2.78 0.96 0 3.55E−02 −2.55 5.8 hsa-asg-5889 2.77 2.05 2.51 3.420.81 0 1.79E−02 −2.55 5.8 hsa-asg-11565 3.27 3.40 4.29 3.86 0.71 119.36E−03 −2.54 5.8 hsa-asg-13013 2.47 2.58 2.71 2.44 0.33 0 8.27E−05−2.53 5.8 hsa-asg-2041 5.10 5.75 4.87 5.59 0.40 33 3.61E−04 −2.49 5.6hsa-asg-1169 4.62 5.55 5.38 5.44 0.69 22 9.46E−03 −2.48 5.6 hsa-miR-2172.77 2.89 2.71 3.05 0.46 0 8.90E−04 −2.47 5.5 hsa-asg-7460 3.03 2.743.84 3.50 0.82 0 2.13E−02 −2.46 5.5 hsa-asg-9681 4.05 3.29 4.51 4.130.75 0 1.43E−02 −2.45 5.5 hsa-asg-1293 5.49 5.76 5.34 6.04 0.52 562.08E−03 −2.44 5.4 hsa-asg-202 3.75 4.01 4.41 4.26 0.74 11 1.47E−02−2.42 5.4 hsa-miR-522 2.47 3.40 2.89 3.05 0.38 0 3.21E−04 −2.42 5.3hsa-asg-2074 2.62 3.17 2.71 3.05 0.55 0 3.03E−03 −2.42 5.3 hsa-asg-36143.83 4.73 4.61 4.49 0.59 0 4.75E−03 −2.41 5.3 hsa-asg-14176 5.44 4.194.13 5.46 0.88 44 3.12E−02 −2.41 5.3 hsa-asg-11977 5.07 5.44 5.30 5.890.77 56 1.77E−02 −2.41 5.3 hsa-asg-6234 9.05 9.49 8.03 9.69 0.94 894.27E−02 −2.40 5.3 hsa-asg-14006 4.47 5.33 4.37 4.78 0.41 0 5.65E−04−2.37 5.2 hsa-cand659 7.49 7.61 7.81 7.78 0.25 100 2.07E−05 −2.36 5.1hsa-asg-4069 4.12 3.56 3.89 4.12 0.67 0 1.05E−02 −2.36 5.1 hsa-asg-92095.74 6.55 6.38 6.38 0.39 67 4.81E−04 −2.35 5.1 hsa-asg-8557 5.84 5.154.84 5.46 0.45 0 1.21E−03 −2.34 5.1 hsa-asg-3783 4.42 5.71 6.54 5.750.95 33 4.77E−02 −2.33 5.0 hsa-miR-499 4.80 4.55 3.84 4.87 0.85 223.20E−02 −2.33 5.0 hsa-asg-9196 1.96 3.17 3.94 3.28 0.90 0 4.03E−02−2.33 5.0 hsa-asg-4207 4.52 4.32 3.62 4.78 0.71 0 1.47E−02 −2.33 5.0hsa-asg-11847 5.69 6.37 6.11 6.53 0.70 67 1.35E−02 −2.32 5.0hsa-asg-7140 6.02 5.86 5.64 6.02 0.46 78 1.33E−03 −2.32 5.0 hsa-asg-19343.03 2.23 3.36 2.87 0.73 0 1.74E−02 −2.31 5.0 hsa-asg-6282 5.62 5.925.00 6.01 0.82 56 2.87E−02 −2.31 5.0 hsa-miR-370 4.31 4.47 5.09 4.840.36 0 2.85E−04 −2.29 4.9 hsa-asg-8636 5.64 6.60 6.45 6.14 0.54 673.71E−03 −2.29 4.9 hsa-asg-9143 6.08 6.69 6.51 6.87 0.70 0 1.45E−02−2.28 4.9 hsa-asg-8280 5.03 6.30 4.21 5.18 0.90 33 4.40E−02 −2.28 4.8hsa-asg-11992 6.72 7.78 6.76 6.99 0.85 89 3.42E−02 −2.27 4.8hsa-asg-2311 3.03 4.87 4.73 4.66 0.72 11 1.71E−02 −2.27 4.8 hsa-asg-71703.48 4.44 3.50 3.91 0.49 0 2.35E−03 −2.25 4.8 hsa-asg-3168 2.77 3.292.89 3.13 0.80 0 2.87E−02 −2.25 4.7 hsa-asg-8404 3.57 3.40 4.58 3.600.70 0 1.66E−02 −2.24 4.7 hsa-miR-212 4.57 4.95 4.73 5.36 0.61 118.67E−03 −2.24 4.7 hsa-asg-10500 3.75 4.38 4.70 3.87 0.75 11 2.18E−02−2.23 4.7 hsa-asg-1174 6.44 7.06 7.01 7.17 0.57 100 5.99E−03 −2.21 4.6hsa-miR-9* 4.92 4.71 5.20 5.55 0.53 44 4.37E−03 −2.21 4.6 hsa-cand7052.90 3.17 1.83 2.63 0.40 0 7.61E−04 −2.20 4.6 hsa-asg-3376 2.47 4.193.36 3.82 0.77 0 2.78E−02 −2.19 4.6 hsa-asg-1966 3.15 4.71 4.37 4.500.66 0 1.45E−02 −2.18 4.5 hsa-asg-1621 5.10 6.68 7.03 6.48 0.70 671.88E−02 −2.16 4.5 hsa-asg-7465 5.14 5.20 5.02 4.86 0.66 11 1.53E−02−2.15 4.4 hsa-asg-14066 5.88 6.19 6.34 6.55 0.53 89 5.03E−03 −2.14 4.4hsa-asg-10883 6.96 5.72 6.61 7.13 0.78 89 3.33E−02 −2.12 4.3hsa-asg-4439 2.77 3.71 4.04 3.31 0.84 0 4.41E−02 −2.11 4.3 hsa-miR1663.98 4.15 4.84 4.91 0.64 0 1.43E−02 −2.11 4.3 hsa-miR219 3.15 4.63 4.414.32 0.81 0 3.85E−02 −2.11 4.3 hsa-asg-1168 5.57 5.55 5.74 6.00 0.55 446.74E−03 −2.10 4.3 hsa-asg-4597 6.83 7.30 6.42 7.49 0.72 0 2.52E−02−2.10 4.3 hsa-miR-518c* 3.66 3.71 4.64 4.02 0.65 0 1.60E−02 −2.10 4.3hsa-miR-372 4.18 3.97 3.22 3.93 0.44 0 2.08E−03 −2.09 4.3 hsa-asg-19196.55 7.29 6.89 7.16 0.61 89 1.20E−02 −2.09 4.3 hsa-asg-4070 6.00 6.246.24 6.64 0.84 89 4.62E−02 −2.08 4.2 hsa-asg-3746 7.08 7.30 6.93 7.620.73 100 2.78E−02 −2.08 4.2 hsa-asg-4155 4.80 5.70 5.73 5.76 0.66 441.81E−02 −2.07 4.2 hsa-asg-9257 5.00 6.28 6.18 5.65 0.72 67 2.58E−02−2.06 4.2 hsa-miR-371 3.83 3.61 2.89 3.66 0.37 0 7.97E−04 −2.05 4.1hsa-asg-11883 6.11 4.55 6.42 6.06 0.64 78 1.69E−02 −2.03 4.1hsa-asg-10414 6.56 7.33 7.55 7.28 0.75 89 3.32E−02 −2.03 4.1hsa-asg-13613 6.98 7.04 7.12 7.71 0.74 100 3.23E−02 −2.03 4.1hsa-asg-8597 5.55 5.71 5.56 5.48 0.31 0 2.91E−04 −2.01 4.0 hsa-asg-92487.43 8.10 7.13 8.14 0.72 100 3.05E−02 −2.00 4.0 hsa-asg-2315 3.66 4.823.89 4.80 0.71 0 2.90E−02 −2.00 4.0 hsa-asg-7043 4.67 4.73 4.84 4.810.70 0 2.69E−02 −1.99 4.0 hsa-asg-8956 5.00 5.92 6.08 6.10 0.77 564.12E−02 −1.96 3.9 hsa-asg-7764 7.82 8.09 7.66 8.28 0.68 11 2.60E−02−1.96 3.9 hsa-asg-10638 8.31 8.70 8.51 8.70 0.58 100 1.29E−02 −1.96 3.9hsa-miR-7 8.64 8.56 8.46 8.60 0.64 100 1.96E−02 −1.95 3.9 hsa-asg-32915.82 6.72 6.17 6.29 0.42 44 2.38E−03 −1.95 3.9 hsa-asg-1860 8.57 8.767.57 8.86 0.77 56 4.49E−02 −1.94 3.8 hsa-asg-6813 3.48 3.71 4.51 4.420.78 22 4.71E−02 −1.93 3.8 hsa-asg-11619 6.14 6.56 7.08 6.89 0.62 1001.78E−02 −1.93 3.8 hsa-asg-13052 3.15 3.88 3.50 3.61 0.57 0 1.21E−02−1.92 3.8 hsa-asg-1462 3.38 4.38 3.73 4.04 0.33 0 5.09E−04 −1.92 3.8hsa-asg-5326 5.52 6.55 5.32 6.29 0.63 67 2.05E−02 −1.92 3.8 hsa-miR-6607.78 7.90 8.59 8.51 0.55 100 1.12E−02 −1.92 3.8 hsa-miR-373 3.57 4.193.36 4.02 0.37 0 1.19E−03 −1.91 3.7 hsa-asg-7690 3.03 3.80 3.84 4.140.63 11 2.04E−02 −1.90 3.7 hsa-asg-2049 2.77 3.51 2.29 3.17 0.56 01.18E−02 −1.90 3.7 hsa-asg-2290 6.89 7.73 7.45 7.86 0.63 0 2.21E−02−1.88 3.7 hsa-asg-7240 5.10 5.58 5.42 5.75 0.48 11 5.93E−03 −1.87 3.7hsa-asg-12964 3.66 3.40 4.21 3.75 0.71 0 3.67E−02 −1.87 3.7hsa-asg-13780 3.03 3.29 4.21 3.17 0.64 0 2.42E−02 −1.87 3.7 hsa-miR-93.48 3.29 4.13 3.29 0.55 0 1.21E−02 −1.87 3.6 hsa-cand208 1.78 3.97 2.513.03 0.66 0 2.88E−02 −1.84 3.6 hsa-asg-3073 5.14 5.77 5.36 5.51 0.45 334.75E−03 −1.84 3.6 hsa-asg-11155 6.59 7.12 6.97 7.48 0.71 56 4.09E−02−1.82 3.5 hsa-asg-1617 6.12 6.70 7.39 6.97 0.71 89 4.33E−02 −1.81 3.5hsa-asg-12069 6.21 7.32 7.35 6.91 0.70 78 4.09E−02 −1.80 3.5 hsa-miR-5014.47 4.25 4.37 4.41 0.70 0 4.18E−02 −1.80 3.5 hsa-asg-5413 4.71 5.305.40 5.66 0.62 33 2.56E−02 −1.79 3.5 hsa-asg-8545 3.83 5.13 4.44 4.840.73 22 4.82E−02 −1.78 3.4 hsa-asg-92 3.83 4.19 5.11 4.48 0.61 112.49E−02 −1.77 3.4 hsa-asg-14155 3.75 3.80 3.36 3.68 0.49 0 9.09E−03−1.76 3.4 hsa-asg-1638 3.48 2.58 4.04 3.60 0.55 0 1.73E−02 −1.75 3.4hsa-asg-9666 4.05 5.28 3.79 4.63 0.66 0 3.61E−02 −1.74 3.3 hsa-asg-29967.68 8.04 7.43 8.10 0.51 0 1.16E−02 −1.74 3.3 hsa-miR-215 5.49 5.09 5.205.69 0.71 33 4.85E−02 −1.74 3.3 hsa-asg-12874 4.18 4.50 4.64 4.42 0.33 01.16E−03 −1.73 3.3 hsa-asg-13754 3.48 3.97 4.21 3.76 0.58 0 2.18E−02−1.73 3.3 hsa-asg-7681 3.98 5.16 5.20 4.84 0.69 11 4.57E−02 −1.71 3.3hsa-asg-4027 7.05 7.88 7.47 7.82 0.53 100 1.60E−02 −1.71 3.3 hsa-miR1027.93 8.39 8.40 8.35 0.37 100 2.51E−03 −1.71 3.3 hsa-miR-128a 9.00 8.758.66 9.33 0.70 100 4.86E−02 −1.71 3.3 hsa-asg-8682 3.75 4.61 4.21 4.460.42 0 4.99E−03 −1.70 3.2 hsa-asg-954 8.02 7.93 7.49 8.38 0.52 1001.59E−02 −1.68 3.2 hsa-asg-1398 4.84 5.43 5.57 5.20 0.37 11 2.80E−03−1.68 3.2 hsa-asg-1923 5.86 5.16 6.16 6.14 0.43 78 6.06E−03 −1.67 3.2hsa-asg-9164 6.60 7.11 7.15 7.18 0.67 89 4.52E−02 −1.66 3.2 hsa-asg-74715.24 4.97 6.07 5.65 0.52 56 1.70E−02 −1.64 3.1 hsa-asg-748 4.18 4.554.44 4.68 0.66 11 4.76E−02 −1.64 3.1 hsa-asg-9320 4.88 4.80 5.28 4.790.60 11 3.29E−02 −1.63 3.1 hsa-asg-8641 4.92 5.65 5.61 5.53 0.66 224.68E−02 −1.63 3.1 hsa-asg-6924 6.35 5.96 6.54 6.55 0.48 78 1.24E−02−1.61 3.0 hsa-asg-4100 4.80 5.09 4.82 5.03 0.30 0 1.03E−03 −1.61 3.0hsa-asg-1115 5.17 4.75 5.16 5.29 0.38 11 4.12E−03 −1.59 3.0 hsa-asg-46376.05 6.48 5.91 6.42 0.33 100 1.80E−03 −1.58 3.0 hsa-asg-7278 3.98 4.735.42 5.02 0.58 11 3.22E−02 −1.58 3.0 hsa-asg-4031 7.59 8.44 7.66 7.990.48 100 1.51E−02 −1.56 2.9 hsa-asg-8508 4.47 4.50 5.07 4.62 0.50 111.91E−02 −1.55 2.9 hsa-asg-3587 4.42 4.35 4.61 4.94 0.54 0 2.61E−02−1.55 2.9 hsa-miR-500 8.11 8.41 8.49 8.41 0.35 100 2.88E−03 −1.54 2.9hsa-asg-11402 5.33 5.65 5.97 5.74 0.34 78 2.48E−03 −1.54 2.9hsa-asg-13027 2.77 4.25 2.51 3.41 0.61 0 4.42E−02 −1.54 2.9hsa-miR-376a* 3.75 3.97 4.21 4.46 0.53 0 2.57E−02 −1.52 2.9 hsa-asg-74595.30 6.20 5.44 5.54 0.54 22 2.95E−02 −1.50 2.8 hsa-asg-2542 4.96 5.285.30 4.87 0.50 0 2.22E−02 −1.48 2.8 hsa-asg-5740 4.62 4.25 4.87 5.100.51 22 2.51E−02 −1.47 2.8 hsa-asg-9885 4.80 5.60 5.24 5.69 0.58 444.63E−02 −1.44 2.7 hsa-miR-566 5.55 6.02 5.42 5.89 0.47 56 2.15E−02−1.42 2.7 hsa-asg-6250 4.80 5.92 6.01 5.48 0.50 0 2.94E−02 −1.40 2.6hsa-asg-5827 4.76 5.16 4.70 5.22 0.42 22 1.38E−02 −1.39 2.6 hsa-miR1973.83 3.51 4.37 4.13 0.30 0 2.16E−03 −1.39 2.6 hsa-asg-10335 4.67 5.074.73 5.08 0.26 0 9.15E−04 −1.39 2.6 hsa-asg-11397 3.27 3.51 3.36 3.660.31 0 2.97E−03 −1.37 2.6 hsa-asg-11900 6.12 6.22 5.36 5.69 0.45 562.23E−02 −1.33 2.5 hsa-asg-8392 5.00 5.51 5.16 5.25 0.49 11 3.34E−02−1.32 2.5 hsa-asg-414 6.60 5.72 5.90 6.27 0.37 78 1.01E−02 −1.30 2.5hsa-asg-7215 4.71 5.40 4.97 5.32 0.47 11 3.43E−02 −1.26 2.4 hsa-asg-83444.71 4.19 3.84 4.44 0.49 0 4.57E−02 −1.23 2.3 hsa-asg-13135 5.14 4.445.57 5.11 0.44 22 3.04E−02 −1.21 2.3 hsa-asg-3826 3.48 3.80 3.73 3.960.39 0 1.94E−02 −1.20 2.3 hsa-asg-7104 4.36 4.71 4.51 4.51 0.32 08.82E−03 −1.18 2.3 hsa-asg-2584 3.66 4.38 3.84 3.95 0.41 0 2.91E−02−1.15 2.2 hsa-asg-4246 4.25 5.30 4.58 4.87 0.38 0 2.24E−02 −1.12 2.2hsa-asg-3969 5.88 6.13 6.49 6.18 0.42 89 3.56E−02 −1.12 2.2 hsa-miR-3626.93 6.55 7.04 7.30 0.43 100 4.15E−02 −1.10 2.1 hsa-asg-10626 4.84 4.254.97 4.84 0.39 0 3.29E−02 −1.06 2.1 hsa-asg-6186 5.07 5.60 5.20 5.600.34 44 1.91E−02 −1.05 2.1 hsa-asg-11729 8.00 8.02 7.68 8.10 0.30 781.16E−02 −1.03 2.0 hsa-miR-584 5.24 5.88 5.82 5.85 0.31 67 1.38E−02−1.02 2.0 SD, standard deviation; %, percentage of samples expressingmiRNA above background levels; Log2Diff, difference in VSN-transformedexpression bewteen TES and LNneg samples.

Example 31 miRNAs Differentally Expressed Between Normal Uterus Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous uterus cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal uterustissue than in cancer-negative lymph nodes.

Total RNA, purified from one normal human uterus sample (FirstChoice®Human Uterus Total RNA, cat. no. AM7892; Ambion, Austin, Tex., USA) andfrom nine normal human lymph node samples (FirstChoice® Human Lymph NodeTotal RNA, cat. no. AM7894; Ambion, Austin, Tex., USA) was purchasedfrom Ambion. miRNA purification and labeling and microarray expressionanalyses were performed as described above in Example 2.

The inventors identified 120 miRNAs (64 verified, 56 predicted) withsignificant differential expression between the normal uterus and normallymph nodes (|log 2Diff| uterus vs LN neg≧1 and p-value≦0.05) (Table30). Among the verified miRNAs, 5 miRNAs (hsa-miR-411, -299-5p, -381,-495 and -204) were expressed at levels between 20- and 25-fold higherin the uterus sample; 16 miRNAs were expressed at levels between 10- and20-fold higher in the uterus sample; and 16 miRNAs were expressed atlevels between 5- and 10-fold higher in the uterus sample thann in thenormal lymph nodes. Among the predicted miRNAs, hsa-asg-13404 st2 andhsa-miR202_st2 were expressed at levels between 25- and 35-fold higherin the uterus sample; 6 miRNA (hsa-asg-5021_st1, -13254 st1, -11443_st1,-5617_st1, -14065_st2 and hsa-miR144 st2) were expressed at levelsbetween 10- and 20-fold higher in the uterus; and 14 miRNAs wereexpressed at levels between 5- and 10-fold higher in the uterus samplethan in normal lymph nodes.

TABLE 30 MicroRNAs Differentially Expressed Between Normal Uterus (UTR)and Cancer-Negative Lymph Node Samples (LNneg) from Patients. ProbeLNneg MicroRNA ID UTR 1 2 3 4 5 6 hsa-asg-13404 Has-asg-13404_st2 7.884.15 2.67 5.28 2.90 3.24 1.57 hsa-miR202 Has-miR202_st2 8.26 3.83 2.674.93 3.94 3.59 2.40 hsa-miR-411 Has-miR-411_st1 8.44 4.84 3.68 4.54 5.225.52 2.94 hsa-miR-299-5p Has-miR-299-5p_st2 9.20 5.71 4.44 5.81 6.375.96 2.94 hsa-miR-381 Has-miR-381_st2 7.55 2.75 2.32 4.21 3.38 4.17 2.94hsa-miR-495 Has-miR-495_st1 9.65 6.01 4.76 6.97 6.25 6.29 4.38hsa-miR-204 Has-miR-204_st2 10.56 7.17 5.77 5.98 8.20 6.73 5.40hsa-miR-493-5p Has-miR-493-5p_st1 8.97 4.35 3.78 6.89 6.18 6.30 3.63hsa-asg-5021 Has-asg-5021_st1 8.89 5.17 4.38 7.00 6.05 5.88 3.48hsa-miR-189 Has-miR-189_st1 9.49 6.07 5.74 4.93 7.23 6.15 4.49hsa-miR-368 Has-miR-368_st2 10.36 6.48 6.47 7.70 8.08 7.68 5.18hsa-asg-13254 Has-asg-13254_st1 8.95 5.58 5.32 6.66 5.89 5.87 3.97hsa-asg-11443 Has-asg-11443_st1 7.17 4.15 3.58 2.84 2.66 3.50 2.65hsa-miR-383 Has-miR-383_st1 6.47 3.31 2.83 2.40 2.57 3.18 1.30hsa-miR-187 Has-miR-187_st1 7.31 4.74 2.75 2.74 3.75 3.18 3.32 hsa-miR-9Has-miR-9_st2 7.22 3.42 3.48 2.84 3.25 3.59 2.14 hsa-miR-9Has-cand90_st2 6.28 3.53 3.11 2.63 2.98 2.79 2.14 hsa-miR-487bHas-miR-487b_st2 9.42 6.05 5.79 6.97 6.90 6.22 5.34 hsa-asg-5617Has-asg-5617_st1 6.61 3.73 3.24 3.90 3.60 3.40 2.65 hsa-miR-127Has-miR-127_st1 10.28 6.80 5.52 7.96 7.53 7.66 5.66 hsa-miR-133aHas-miR-133a_st1 11.31 7.76 7.65 5.93 10.81 8.16 7.08 hsa-asg-14065Has-asg-14065_st2 6.46 1.24 2.58 5.31 4.62 1.80 2.49 hsa-miR-376aHas-miR-376a_st2 8.01 5.13 4.03 5.49 5.66 4.82 3.97 hsa-miR-376aHas-asg-10049_st1 8.29 5.01 4.85 6.09 5.55 5.31 4.05 hsa-miR-376aHas-asg-14234_st1 6.86 3.31 4.25 3.38 3.98 4.82 3.63 hsa-miR-424Has-miR-424_st1 9.28 5.79 6.11 5.53 5.91 5.75 3.43 hsa-miR-450Has-asg-14228_st2 6.37 3.53 2.13 2.40 2.98 2.49 3.08 hsa-miR-450hsa-miR-450_st2 6.92 4.22 2.67 2.84 3.89 3.59 3.97 hsa-miR-582hsa-miR-582_st1 8.06 5.84 2.13 4.54 5.95 4.86 4.05 hsa-miR144hsa-miR144_st2 6.87 4.59 4.38 2.63 3.38 2.65 2.65 hsa-miR-9*hsa-miR-9-_st2 9.03 6.19 6.05 5.57 6.10 5.37 5.81 hsa-miR-299-3phsa-asg-10378_st1 6.20 3.64 3.36 3.21 1.90 2.41 1.95 hsa-miR-299-3phsa-miR-299-3p_st2 5.99 2.41 3.36 3.38 2.90 3.40 2.73 hsa-miR-154hsa-miR-154_st2 6.83 3.73 2.50 4.30 4.35 4.88 3.08 hsa-miR-132hsa-miR-132_st1 11.65 9.71 8.51 8.34 8.34 8.56 8.02 hsa-miR-101hsa-miR-101_st1 8.78 7.17 6.48 4.21 5.98 6.49 4.65 hsa-asg-13284hsa-asg-13284_st1 7.13 4.88 2.03 3.59 5.83 4.82 2.80 hsa-miR-379hsa-miR-379_st1 9.81 6.51 6.37 7.12 6.96 7.01 6.54 hsa-cand206hsa-cand206_st1 5.88 2.22 1.94 3.78 3.60 2.65 2.14 hsa-miR-134hsa-miR-134_st2 8.35 5.09 5.73 6.00 5.84 5.67 4.15 hsa-asg-14176hsa-asg-14176_st1 8.64 6.05 5.67 5.28 6.77 6.23 5.37 hsa-miR-432hsa-miR-432_st2 7.95 4.53 5.38 5.93 5.52 5.74 5.62 hsa-asg-10202hsa-asg-10202_st2 6.24 4.69 2.03 3.30 5.24 3.06 1.95 hsa-miR-149hsa-miR-149_st2 6.13 4.47 3.82 1.76 4.14 2.72 1.48 hsa-asg-9696hsa-asg-9696_st1 9.45 7.42 6.31 6.68 7.04 6.23 6.22 hsa-miR-19ahsa-miR-19a_st1 5.76 4.59 3.36 2.84 3.12 3.59 2.49 hsa-asg-10883hsa-asg-10883_st1 10.05 7.63 7.13 7.34 8.22 8.00 6.52 hsa-cand660hsa-cand660_st1 5.05 2.22 1.74 1.37 1.48 2.65 1.57 hsa-asg-13166hsa-asg-13166_st2 6.81 4.47 3.36 4.87 4.94 4.07 4.12 hsa-miR-485-5phsa-miR-485-5p_st2 5.89 3.42 3.24 3.38 3.66 3.24 1.95 hsa-asg-9514hsa-asg-9514_st1 6.22 2.58 4.50 3.21 2.48 4.33 3.63 hsa-miR-369-5phsa-miR-369-5p_st2 6.16 3.53 2.67 4.46 4.35 4.33 2.49 hsa-miR-369-5phsa-asg-1521_st1 5.56 3.73 3.36 4.01 2.20 3.68 3.68 hsa-miR-212hsa-miR-212_st2 8.09 6.26 5.68 6.18 5.71 5.14 5.05 hsa-miR-140hsa-miR-140_st2 8.74 7.72 6.11 6.37 7.21 6.60 5.45 hsa-cand207hsa-cand207_st1 5.83 2.58 4.11 3.66 2.48 3.64 3.63 hsa-miR-433hsa-miR-433_st2 5.37 2.41 2.67 4.54 2.90 4.17 2.32 hsa-asg-3036hsa-asg-3036_st1 6.15 3.18 3.73 5.12 3.05 2.65 2.49 hsa-miR-218hsa-miR-218_st2 11.24 9.61 9.22 8.83 9.68 9.13 8.59 hsa-miR-660hsa-miR-660_st1 10.96 9.28 8.94 7.94 9.15 8.60 8.38 hsa-miR-485-3phsa-miR23_st2 5.66 4.29 2.50 4.11 3.25 2.41 3.20 hsa-asg-7465hsa-asg-7465_st2 7.27 4.64 4.31 4.01 6.17 5.05 4.15 hsa-asg-6277hsa-asg-6277_st2 8.40 6.88 6.93 5.07 5.94 6.46 6.96 hsa-miR-410hsa-miR-410_st1 4.89 2.41 2.32 1.37 3.71 2.49 2.80 hsa-asg-12144hsa-asg-12144_st1 5.17 2.90 3.11 1.63 2.90 3.45 3.43 hsa-miR-154*hsa-miR-154-_st2 6.62 5.81 3.48 4.21 4.98 4.59 3.89 hsa-asg-9269hsa-asg-9269_st2 5.28 2.58 2.50 4.68 2.90 2.57 2.65 hsa-miR-503hsa-miR-503_st1 8.90 6.88 7.17 7.38 6.60 6.08 6.45 hsa-miR-503hsa-miR141_st1 8.98 7.10 7.28 7.30 6.52 6.55 6.62 hsa-miR-499hsa-miR12_st1 7.13 5.44 4.76 5.37 6.66 4.07 4.38 hsa-miR-499hsa-miR-499_st1 6.86 5.31 4.61 4.75 6.57 4.49 4.12 hsa-miR-339hsa-miR-339_st1 7.75 6.65 5.92 5.21 6.67 5.25 5.07 hsa-miR-501hsa-miR-501_st1 6.65 5.24 4.81 2.84 5.09 4.04 4.60 hsa-asg-1203hsa-asg-1203_st1 5.94 4.47 4.31 2.63 3.80 4.17 4.49 hsa-miR-497hsa-miR43_st1 10.24 8.19 8.51 7.52 9.37 8.85 7.73 hsa-miR-382hsa-miR-382_st1 8.85 7.16 6.80 7.35 7.13 7.40 6.51 hsa-miR-542-5phsa-miR-542-5p_st2 5.94 3.91 2.83 3.66 3.60 3.81 2.80 hsa-asg-12964hsa-asg-12964_st2 5.89 4.88 2.83 4.01 4.25 3.89 2.65 hsa-asg-14174hsa-asg-14174_st1 4.82 2.90 2.32 2.63 3.50 3.68 2.49 hsa-miR-214hsa-miR-214_st1 11.71 9.75 9.50 9.77 10.99 10.32 9.88 hsa-asg-13308hsa-asg-13308_st2 11.51 10.57 9.41 9.59 9.80 9.15 8.58 hsa-asg-279hsa-asg-279_st2 8.03 6.41 6.02 5.71 6.88 6.82 4.79 hsa-asg-14230hsa-asg-14230_st1 5.92 4.29 3.68 4.38 4.56 3.29 3.43 hsa-asg-5304hsa-asg-5304_st1 11.61 10.61 9.43 9.76 10.13 9.37 8.76 hsa-miR102hsa-miR102_st2 10.40 8.89 8.26 8.71 8.69 8.11 7.77 hsa-asg-13471hsa-asg-13471_st1 6.66 4.74 4.71 4.38 5.11 5.31 5.34 hsa-asg-5827hsa-asg-5827_st2 7.26 5.61 4.71 5.71 5.52 5.65 5.15 hsa-miR-19bhsa-miR-19b_st2 9.46 8.37 8.28 7.32 8.02 8.01 6.45 hsa-asg-13304hsa-asg-13304_st2 8.37 6.61 6.44 6.49 7.56 7.42 5.62 hsa-asg-9687hsa-asg-9687_st1 6.09 4.74 4.76 3.90 4.65 3.85 4.19 hsa-asg-6543hsa-asg-6543_st1 5.82 4.22 3.58 4.11 4.25 4.30 3.89 hsa-asg-3145hsa-asg-3145_st1 7.38 5.66 5.14 5.28 6.40 6.22 5.53 hsa-miR-345hsa-miR-345_st2 8.24 6.72 7.33 6.32 6.46 6.64 6.97 hsa-miR-494hsa-miR-494_st1 8.57 6.39 7.25 7.23 6.95 6.60 7.61 hsa-miR-500hsa-miR-500_st1 10.22 8.74 8.38 8.78 8.81 8.16 7.79 hsa-miR-455hsa-miR-455_st1 6.99 5.81 4.11 5.81 6.00 5.67 4.70 hsa-miR-222hsa-miR-222_st2 11.47 10.40 9.52 9.55 10.21 9.72 9.28 hsa-miR-130ahsa-cand144_st1 11.87 10.46 9.80 9.72 10.94 10.77 9.20 hsa-miR-130ahsa-miR-130a_st1 11.70 10.33 9.91 9.72 10.91 10.76 9.16 hsa-miR-151hsa-miR-151_st2 11.11 10.19 9.71 9.15 9.80 9.35 9.42 hsa-miR-99bhsa-miR-99b_st1 12.26 10.99 10.46 10.53 11.45 11.14 10.0 hsa-asg-3267hsa-asg-3267_st1 9.00 7.72 7.79 6.33 6.76 8.05 7.58 hsa-asg-8673hsa-asg-8673_st1 5.20 3.91 2.50 3.66 4.32 3.97 2.80 hsa-asg-10674hsa-asg-10674_st1 6.52 4.29 4.44 5.24 5.41 5.17 5.25 hsa-miR-362hsa-miR-362_st1 8.97 7.42 7.66 7.94 7.60 7.44 7.08 hsa-asg-14233hsa-asg-14233_st1 10.87 9.44 9.21 9.23 10.14 9.41 8.84 hsa-asg-8872hsa-asg-8872_st1 8.78 7.85 7.97 6.91 6.99 7.39 7.83 hsa-miR-28hsa-miR-28_st1 11.99 11.14 10.69 10.10 11.08 10.81 10.0 hsa-asg-13270hsa-asg-13270_st2 5.49 3.91 3.78 3.78 4.96 4.57 4.25 hsa-asg-317hsa-asg-317_st2 6.17 5.31 4.98 5.04 4.92 4.38 3.81 hsa-miR-542-3phsa-miR-542-3p_st1 7.05 5.09 5.25 6.01 6.29 5.71 5.62 hsa-miR257hsa-miR257_st1 10.28 9.61 9.05 9.23 9.55 8.84 8.49 hsa-asg-12346hsa-asg-12346_st2 8.18 6.78 6.93 7.19 7.29 6.95 6.47 hsa-asg-12515hsa-asg-12515_st2 5.44 4.00 4.25 3.04 4.10 4.57 4.32 hsa-asg-10113hsa-asg-10113_st1 5.76 5.01 4.03 4.46 4.59 3.50 3.81 hsa-miR-320hsa-miR-320_st1 12.27 11.28 11.14 11.24 11.40 11.46 10.7 hsa-asg-9347hsa-asg-9347_st2 5.05 4.35 3.95 3.90 4.28 3.59 3.43 hsa-miR-628hsa-miR-628_st2 8.98 8.29 7.83 7.21 8.00 7.32 7.47 hsa-asg-7471hsa-asg-7471_st1 7.02 5.58 5.67 5.68 6.74 5.67 5.25 hsa-miR-566hsa-miR-566_st1 7.25 6.72 6.12 5.12 5.83 6.11 6.10 hsa-asg-11181hsa-asg-11181_st1 6.90 5.66 5.19 6.13 6.30 5.56 5.54 hsa-asg-2301hsa-asg-2301_st2 8.04 7.01 6.27 6.73 7.45 6.92 6.59 hsa-miR-510hsa-miR-510_st2 6.09 5.27 5.02 4.75 4.65 4.71 5.04 hsa-asg-6618hsa-asg-6618_st2 6.60 5.47 5.94 4.50 5.31 5.71 6.06 hsa-miR-454-3phsa-asg-11541_st2 9.20 8.62 8.26 8.54 8.45 7.84 7.87 hsa-asg-4637hsa-asg-4637_st1 7.55 7.08 6.44 6.38 6.52 6.47 6.47 hsa-asg-12096hsa-asg-12096_st2 5.29 4.64 3.86 4.11 4.48 4.07 4.38 hsa-asg-3038hsa-asg-3038_st2 6.68 5.84 5.79 5.45 5.90 5.89 5.94 hsa-miR-361hsa-miR-361_st2 12.47 11.73 11.57 11.28 12.07 11.67 11.6 LNneg MicroRNA7 8 9 M SD % ttest log2Diff Fold hsa-asg-13404 2.14 1.09 2.29 2.82 1.290 5.86E−03 −5.06 33.4 hsa-miR202 4.25 2.41 3.06 3.45 0.88 0 8.22E−04−4.81 28.0 hsa-miR-411 3.38 2.23 2.07 3.83 1.27 11 8.92E−03 −4.61 24.4hsa-miR-299-5p 5.03 2.41 3.94 4.74 1.40 44 1.64E−02 −4.47 22.1hsa-miR-381 3.48 1.28 3.22 3.08 0.91 0 1.69E−03 −4.46 22.0 hsa-miR-4955.14 2.41 4.87 5.23 1.36 56 1.51E−02 −4.42 21.5 hsa-miR-204 5.17 5.405.76 6.18 1.00 44 3.15E−03 −4.38 20.9 hsa-miR-493-5p 4.36 1.85 4.58 4.661.58 33 3.17E−02 −4.32 19.9 hsa-asg-5021 4.57 3.03 2.07 4.63 1.58 223.37E−02 −4.26 19.2 hsa-miR-189 4.92 3.51 4.92 5.33 1.09 44 6.79E−03−4.16 17.9 hsa-miR-368 5.62 3.97 5.07 6.25 1.40 67 2.40E−02 −4.11 17.2hsa-asg-13254 4.71 2.41 3.36 4.86 1.37 56 2.25E−02 −4.08 16.9hsa-asg-11443 3.38 2.74 2.29 3.09 0.59 0 1.81E−04 −4.08 16.9 hsa-miR-3832.62 2.58 1.36 2.46 0.71 0 6.53E−04 −4.01 16.1 hsa-miR-187 3.03 3.353.14 3.33 0.61 0 2.73E−04 −3.98 15.8 hsa-miR-9 3.48 3.29 4.13 3.29 0.550 1.38E−04 −3.93 15.2 hsa-miR-9 2.62 1.66 3.62 2.79 0.63 0 7.35E−04−3.49 11.3 hsa-miR-487b 4.88 3.71 4.37 5.58 1.11 67 1.11E−02 −3.84 14.3hsa-asg-5617 1.42 1.46 1.60 2.78 1.03 0 7.64E−03 −3.83 14.2 hsa-miR-1276.57 5.47 5.42 6.51 1.03 56 8.51E−03 −3.77 13.7 hsa-miR-133a 8.20 6.236.46 7.59 1.47 89 4.28E−02 −3.72 13.2 hsa-asg-14065 2.90 1.37 2.40 2.751.39 0 3.46E−02 −3.71 13.1 hsa-miR-376a 2.47 3.80 3.73 4.34 1.02 09.25E−03 −3.66 12.7 hsa-miR-376a 3.83 4.12 4.64 4.83 0.75 11 2.39E−03−3.46 11.0 hsa-miR-376a 2.90 4.04 1.83 3.57 0.86 0 6.93E−03 −3.29 9.8hsa-miR-424 5.80 5.70 7.21 5.69 0.98 89 8.43E−03 −3.59 12.0 hsa-miR-4502.90 2.89 2.71 2.79 0.42 0 3.74E−05 −3.58 11.9 hsa-miR-450 4.18 4.444.04 3.76 0.62 0 1.28E−03 −3.15 8.9 hsa-miR-582 4.05 4.32 4.64 4.49 1.1222 1.66E−02 −3.58 11.9 hsa-miR144 2.77 3.17 4.21 3.38 0.81 0 3.42E−03−3.49 11.2 hsa-miR-9* 4.92 4.71 5.20 5.55 0.53 44 2.65E−04 −3.48 11.2hsa-miR-299-3p 1.60 3.17 3.50 2.75 0.78 0 3.05E−03 −3.46 11.0hsa-miR-299-3p 3.27 1.85 2.89 2.91 0.52 0 5.21E−04 −3.08 8.5 hsa-miR-1543.15 1.28 3.94 3.47 1.10 0 2.02E−02 −3.36 10.3 hsa-miR-132 8.00 8.426.86 8.31 0.74 89 2.63E−03 −3.34 10.1 hsa-miR-101 4.84 4.32 5.07 5.471.08 44 1.98E−02 −3.31 9.9 hsa-asg-13284 4.71 3.75 2.40 3.87 1.29 04.26E−02 −3.26 9.6 hsa-miR-379 6.33 6.13 6.20 6.58 0.37 100 3.28E−05−3.23 9.4 hsa-cand206 3.57 1.28 2.71 2.65 0.86 0 7.38E−03 −3.22 9.3hsa-miR-134 4.71 4.29 5.00 5.16 0.68 0 2.25E−03 −3.18 9.1 hsa-asg-141765.44 4.19 4.13 5.46 0.88 44 8.81E−03 −3.18 9.0 hsa-miR-432 3.48 4.252.71 4.80 1.13 22 2.92E−02 −3.16 8.9 hsa-asg-10202 2.47 3.35 1.71 3.091.22 0 4.03E−02 −3.15 8.9 hsa-miR-149 2.47 3.35 3.14 3.04 1.03 02.15E−02 −3.09 8.5 hsa-asg-9696 6.61 6.53 5.07 6.46 0.65 89 2.43E−03−3.00 8.0 hsa-miR-19a 1.60 1.28 2.51 2.82 1.01 0 2.50E−02 −2.93 7.6hsa-asg-10883 6.96 5.72 6.61 7.13 0.78 89 7.50E−03 −2.93 7.6 hsa-cand6602.77 2.58 2.71 2.12 0.58 0 1.37E−03 −2.92 7.6 hsa-asg-13166 3.57 2.054.04 3.94 0.88 0 1.54E−02 −2.86 7.3 hsa-miR-485-5p 3.48 1.85 3.50 3.080.68 0 4.42E−03 −2.81 7.0 hsa-asg-9514 4.18 2.58 3.36 3.43 0.79 09.81E−03 −2.79 6.9 hsa-miR-369-5p 2.90 2.58 3.50 3.42 0.81 0 1.21E−02−2.74 6.7 hsa-miR-369-5p 3.38 2.74 3.50 3.36 0.56 0 5.91E−03 −2.20 4.6hsa-miR-212 4.57 4.95 4.73 5.36 0.61 11 2.95E−03 −2.73 6.6 hsa-miR-1404.76 4.75 5.24 6.02 1.05 44 4.04E−02 −2.71 6.6 hsa-cand207 2.62 2.743.22 3.19 0.60 0 3.01E−03 −2.64 6.2 hsa-miR-433 1.60 1.66 2.89 2.80 1.000 4.08E−02 −2.58 6.0 hsa-asg-3036 3.27 4.73 4.67 3.65 0.96 0 3.97E−02−2.50 5.6 hsa-miR-218 7.75 7.43 8.74 8.77 0.77 100 1.59E−02 −2.47 5.5hsa-miR-660 7.78 7.90 8.59 8.51 0.55 100 3.01E−03 −2.45 5.5hsa-miR-485-3p 3.57 2.74 3.06 3.24 0.66 0 8.27E−03 −2.42 5.4hsa-asg-7465 5.14 5.20 5.02 4.86 0.66 11 8.70E−03 −2.42 5.3 hsa-asg-62774.92 6.32 4.54 6.00 0.94 67 4.17E−02 −2.39 5.3 hsa-miR-410 3.15 1.852.51 2.51 0.68 0 1.07E−02 −2.38 5.2 hsa-asg-12144 3.48 2.05 2.51 2.830.65 0 9.33E−03 −2.34 5.1 hsa-miR-154* 4.25 3.17 4.37 4.31 0.79 112.39E−02 −2.31 5.0 hsa-asg-9269 2.62 2.23 3.94 2.96 0.80 0 2.60E−02−2.31 5.0 hsa-miR-503 6.39 6.60 5.99 6.62 0.46 78 1.61E−03 −2.28 4.9hsa-miR-503 6.85 6.87 6.74 6.87 0.30 100 1.62E−04 −2.11 4.3 hsa-miR-4994.80 4.55 3.84 4.87 0.85 22 3.62E−02 −2.26 4.8 hsa-miR-499 5.03 4.254.58 4.86 0.74 22 3.34E−02 −2.00 4.0 hsa-miR-339 5.00 4.22 5.42 5.490.80 33 2.76E−02 −2.26 4.8 hsa-miR-501 4.47 4.25 4.37 4.41 0.70 01.65E−02 −2.24 4.7 hsa-asg-1203 3.91 3.71 1.83 3.70 0.90 0 4.63E−02−2.24 4.7 hsa-miR-497 7.44 7.11 7.81 8.06 0.73 89 2.25E−02 −2.18 4.5hsa-miR-382 6.27 5.91 5.79 6.70 0.61 78 1.05E−02 −2.15 4.4hsa-miR-542-5p 3.83 4.66 5.16 3.81 0.76 0 2.82E−02 −2.14 4.4hsa-asg-12964 3.66 3.40 4.21 3.75 0.71 0 2.14E−02 −2.13 4.4hsa-asg-14174 2.77 1.46 2.51 2.70 0.65 0 1.48E−02 −2.13 4.4 hsa-miR-2149.26 8.43 8.50 9.60 0.81 100 3.89E−02 −2.11 4.3 hsa-asg-13308 9.50 8.959.09 9.41 0.57 100 8.19E−03 −2.11 4.3 hsa-asg-279 5.31 5.27 6.14 5.930.72 0 2.44E−02 −2.10 4.3 hsa-asg-14230 2.77 3.93 4.04 3.82 0.58 09.00E−03 −2.10 4.3 hsa-asg-5304 9.57 9.06 9.14 9.54 0.57 100 8.65E−03−2.07 4.2 hsa-miR102 7.93 8.39 8.40 8.35 0.37 100 8.24E−04 −2.05 4.1hsa-asg-13471 4.36 4.04 3.50 4.61 0.61 11 1.31E−02 −2.05 4.1hsa-asg-5827 4.76 5.16 4.70 5.22 0.42 22 1.73E−03 −2.04 4.1 hsa-miR-19b6.90 6.51 7.18 7.45 0.74 100 3.31E−02 −2.02 4.0 hsa-asg-13304 6.33 4.976.20 6.41 0.80 67 4.82E−02 −1.96 3.9 hsa-asg-9687 4.05 3.71 3.50 4.150.47 0 4.31E−03 −1.94 3.8 hsa-asg-6543 3.48 3.71 3.36 3.88 0.36 08.88E−04 −1.94 3.8 hsa-asg-3145 4.96 4.44 5.32 5.44 0.61 33 1.63E−02−1.94 3.8 hsa-miR-345 5.10 5.32 6.13 6.33 0.73 78 3.71E−02 −1.91 3.8hsa-miR-494 5.71 5.84 6.58 6.68 0.64 100 2.37E−02 −1.88 3.7 hsa-miR-5008.11 8.41 8.49 8.41 0.35 100 1.11E−03 −1.81 3.5 hsa-miR-455 5.07 5.304.58 5.23 0.66 33 3.42E−02 −1.77 3.4 hsa-miR-222 9.50 9.37 9.84 9.710.38 100 2.27E−03 −1.77 3.4 hsa-miR-130a 9.99 9.54 10.5 10.1 0.60 1002.38E−02 −1.76 3.4 hsa-miR-130a 10.03 9.61 10.4 10.1 0.57 100 2.86E−02−1.60 3.0 hsa-miR-151 9.02 8.50 9.03 9.35 0.50 100 1.06E−02 −1.76 3.4hsa-miR-99b 10.15 9.51 10.3 10.5 0.60 100 2.46E−02 −1.74 3.3hsa-asg-3267 6.67 7.76 6.70 7.26 0.64 0 3.25E−02 −1.74 3.3 hsa-asg-86733.75 3.03 3.50 3.49 0.60 0 2.65E−02 −1.71 3.3 hsa-asg-10674 4.71 4.324.64 4.83 0.44 0 6.60E−03 −1.69 3.2 hsa-miR-362 6.93 6.55 7.04 7.30 0.43100 6.04E−03 −1.67 3.2 hsa-asg-14233 9.03 8.85 8.87 9.23 0.41 1005.63E−03 −1.64 3.1 hsa-asg-8872 6.21 7.06 6.34 7.17 0.64 89 4.57E−02−1.61 3.0 hsa-miR-28 10.10 9.38 10.5 10.4 0.57 100 3.21E−02 −1.56 2.9hsa-asg-13270 3.48 3.80 3.06 3.95 0.57 0 3.40E−02 −1.53 2.9 hsa-asg-3173.91 4.41 5.07 4.65 0.54 33 2.79E−02 −1.52 2.9 hsa-miR-542-3p 5.38 5.884.70 5.55 0.49 78 2.01E−02 −1.51 2.8 hsa-miR257 8.48 8.13 7.89 8.81 0.61100 4.96E−02 −1.48 2.8 hsa-asg-12346 6.20 6.30 6.25 6.71 0.42 229.87E−03 −1.47 2.8 hsa-asg-12515 3.66 4.12 3.73 3.98 0.45 0 1.47E−02−1.47 2.8 hsa-asg-10113 4.71 3.97 4.87 4.33 0.52 11 3.17E−02 −1.43 2.7hsa-miR-320 10.45 10.17 10.0 10.8 0.55 100 4.43E−02 −1.39 2.6hsa-asg-9347 3.03 2.74 3.62 3.66 0.54 0 3.94E−02 −1.39 2.6 hsa-miR-6287.23 7.64 7.43 7.60 0.37 100 7.90E−03 −1.38 2.6 hsa-asg-7471 5.24 4.976.07 5.65 0.52 56 3.64E−02 −1.37 2.6 hsa-miR-566 5.55 6.02 5.42 5.890.47 56 2.55E−02 −1.36 2.6 hsa-asg-11181 5.33 5.03 5.18 5.55 0.43 111.78E−02 −1.35 2.6 hsa-asg-2301 6.44 6.22 6.76 6.71 0.39 100 1.18E−02−1.33 2.5 hsa-miR-510 4.52 4.25 5.07 4.81 0.32 11 5.01E−03 −1.28 2.4hsa-asg-6618 4.92 5.18 5.22 5.37 0.49 22 4.52E−02 −1.23 2.3hsa-miR-454-3p 7.89 7.31 7.80 8.06 0.43 100 3.66E−02 −1.14 2.2hsa-asg-4637 6.05 6.48 5.91 6.42 0.33 100 1.16E−02 −1.12 2.2hsa-asg-12096 4.12 3.88 4.29 4.20 0.26 0 4.43E−03 −1.08 2.1 hsa-asg-30385.38 5.13 5.61 5.66 0.28 44 8.97E−03 −1.02 2.0 hsa-miR-361 11.12 10.9111.1 11.4 0.37 100 3.19E−02 −1.01 2.0 SD, standard deviation; %,percentage of samples expressing miRNA above background levels;Log2Diff, difference in VSN-transformed expression bewteen UTR and LNnegsamples.

Example 32 miRNAs Differentally Expressed Between Normal Spleen Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous spleen cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal spleentissue than in cancer-negative lymph nodes.

Total RNA, purified from one normal human spleen sample (FirstChoice®Human Spleen Total RNA, cat. no. AM7970 Ambion, Austin, Tex., USA) andfrom nine normal human lymph node samples (FirstChoice® Human Lymph NodeTotal RNA, cat. no. AM7894; Ambion, Austin, Tex., USA) was purchasedfrom Ambion. miRNA purification and labeling and microarray expressionanalyses were performed as described above in Example 2.

The inventors identified 133 miRNAs (67 verified, 66 predicted) withsignificant differential expression between the normal spleen and normallymph node samples (|log 2Diff| spleen vs LN neg≧1 and p-value<0.05)(Table 31). Among the verified miRNAs, 7 miRNAs (hsa-miR-495, -493-5p,-493-3p, 338, -368, -487b and -139) were expressed at levels between 20-and 50-fold higher in the spleen sample; 17 miRNAs were expressed atlevels between 10- and 20-fold higher in spleen; and 18 miRNAs wereexpressed at levels between 5- and 10-fold higher in the spleen samplethan in normal lymph nodes. Among the predicted miRNAs, 3 miRNAs(hsa-asg-13404_st2, 13166_st2 and hsa-miR202 st2) were expressed atlevels between 20- and 40-fold higher in the spleen sample; 9 miRNAswere expressed at levels between 10- and 20-fold higher in spleen; and 9miRNAs were expressed at levels between 5- and 10-fold higher in thespleen sample than in normal lymph nodes.

TABLE 31 MicroRNAs Differentially Expressed Between Normal Spleen (SPL)and Cancer-Negative Lymph Node Samples (LNneg) from Patients. ProbeLNneg MicroRNA ID SPL 1 2 3 4 5 6 hsa-miR-495 hsa-miR-495_st1 10.60 6.014.76 6.97 6.25 6.29 4.38 hsa-miR-493-5p hsa-miR-493-5p_st1 9.88 4.353.78 6.89 6.18 6.30 3.63 hsa-asg-13404 hsa-asg-13404_st2 7.94 4.15 2.675.28 2.90 3.24 1.57 hsa-miR-493-3p hsa-asg-8480_st1 6.31 0.56 0.57 2.741.48 1.35 0.64 hsa-miR-493-3p hsa-miR-493-3p_st1 6.65 4.64 3.05 4.062.98 2.24 1.86 hsa-miR-338 hsa-miR-338_st1 9.10 5.09 4.03 5.57 4.85 4.002.14 hsa-miR-368 hsa-miR-368_st2 10.98 6.48 6.47 7.70 8.08 7.68 5.18hsa-miR202 hsa-miR202_st2 8.13 3.83 2.67 4.93 3.94 3.59 2.40hsa-miR-487b hsa-miR-487b_st2 10.14 6.05 5.79 6.97 6.90 6.22 5.34hsa-asg-13166 hsa-asg-13166_st2 8.49 4.47 3.36 4.87 4.94 4.07 4.12hsa-miR-139 hsa-miR-139_st2 13.32 10.99 9.36 9.56 9.74 8.42 8.94hsa-miR-376a hsa-miR-376a_st2 8.74 5.13 4.03 5.49 5.66 4.82 3.97hsa-miR-376a hsa-asg-14234_st1 7.80 3.31 4.25 3.38 3.98 4.82 3.63hsa-miR-411 hsa-miR-411_st1 8.08 4.84 3.68 4.54 5.22 5.52 2.94hsa-miR-539 hsa-miR-539_st1 6.70 2.03 3.11 2.63 3.38 2.65 2.94hsa-miR-539 hsa-miR177_st1 6.35 2.58 2.50 1.37 2.39 1.80 1.86hsa-miR-376a hsa-asg-10049_st1 8.99 5.01 4.85 6.09 5.55 5.31 4.05hsa-asg-5021 hsa-asg-5021_st1 8.78 5.17 4.38 7.00 6.05 5.88 3.48hsa-miR-432 hsa-miR-432_st2 8.93 4.53 5.38 5.93 5.52 5.74 5.62hsa-miR-424 hsa-miR-424_st1 9.80 5.79 6.11 5.53 5.91 5.75 3.43hsa-asg-5617 hsa-asg-5617_st1 6.88 3.73 3.24 3.90 3.60 3.40 2.65hsa-miR-409-3p hsa-miR-409-3p_st1 7.74 2.22 3.42 5.14 4.98 4.73 2.94hsa-miR-369-5p hsa-asg-1521_st1 7.32 3.73 3.36 4.01 2.20 3.68 3.68hsa-miR-369-5p hsa-miR-369-5p_st2 7.14 3.53 2.67 4.46 4.35 4.33 2.49hsa-asg-13254 hsa-asg-13254_st1 8.82 5.58 5.32 6.66 5.89 5.87 3.97hsa-miR-381 hsa-miR-381_st2 6.98 2.75 2.32 4.21 3.38 4.17 2.94hsa-miR-450 hsa-miR-450_st2 7.66 4.22 2.67 2.84 3.89 3.59 3.97hsa-miR-450 hsa-asg-14228_st2 6.54 3.53 2.13 2.40 2.98 2.49 3.08hsa-cand26 hsa-cand26_st2 8.88 6.11 5.56 6.04 4.06 3.64 4.70 hsa-miR-127hsa-miR-127_st1 10.33 6.80 5.52 7.96 7.53 7.66 5.66 hsa-asg-10881hsa-asg-10881_st1 8.98 5.71 4.98 6.06 5.43 4.76 5.40 hsa-asg-13135hsa-asg-13135_st1 8.87 5.61 5.61 5.24 4.89 4.76 4.70 hsa-miR-410hsa-miR-410_st1 6.27 2.41 2.32 1.37 3.71 2.49 2.80 hsa-miR-485-3phsa-miR23_st2 6.93 4.29 2.50 4.11 3.25 2.41 3.20 hsa-miR-485-3phsa-miR-485-3p_st2 6.74 3.64 2.97 4.30 4.41 4.24 3.08 hsa-asg-7472hsa-asg-7472_st2 5.63 1.63 2.13 1.89 2.00 2.86 1.76 hsa-miR-487ahsa-miR-487a_st2 6.14 2.22 2.83 2.63 3.38 2.16 3.20 hsa-miR-487ahsa-asg-6915_st1 6.12 4.07 3.58 3.90 3.38 3.85 3.72 hsa-miR-376bhsa-miR-376b_st1 5.85 1.83 2.13 4.11 3.32 2.65 1.03 hsa-cand206hsa-cand206_st1 6.19 2.22 1.94 3.78 3.60 2.65 2.14 hsa-miR-154hsa-miR-154_st2 6.86 3.73 2.50 4.30 4.35 4.88 3.08 hsa-miR-542-5phsa-miR-542-5p_st2 7.19 3.91 2.83 3.66 3.60 3.81 2.80 hsa-miR-542-3phsa-miR-542-3p_st1 8.91 5.09 5.25 6.01 6.29 5.71 5.62 hsa-asg-8067hsa-asg-8067_st2 9.32 6.66 6.35 5.82 6.62 6.65 5.69 hsa-miR-134hsa-miR-134_st2 8.50 5.09 5.73 6.00 5.84 5.67 4.15 hsa-miR-379hsa-miR-379_st1 9.83 6.51 6.37 7.12 6.96 7.01 6.54 hsa-miR-329hsa-miR-329_st1 6.60 3.73 2.97 4.61 3.25 4.54 2.80 hsa-miR-494hsa-miR-494_st1 9.86 6.39 7.25 7.23 6.95 6.60 7.61 hsa-miR-494hsa-miR61_st2 9.15 6.81 6.50 6.86 6.42 6.35 7.05 hsa-asg-9696hsa-asg-9696_st1 9.61 7.42 6.31 6.68 7.04 6.23 6.22 hsa-miR-503hsa-miR-503_st1 9.76 6.88 7.17 7.38 6.60 6.08 6.45 hsa-miR-503hsa-miR141_st1 9.66 7.10 7.28 7.30 6.52 6.55 6.62 hsa-miR-329hsa-asg-13589_st2 6.29 1.63 3.11 3.78 3.71 4.38 2.32 hsa-miR-329hsa-asg-13204_st2 6.68 4.07 2.83 4.21 3.38 4.20 3.43 hsa-miR-382hsa-miR-382_st1 9.77 7.16 6.80 7.35 7.13 7.40 6.51 hsa-miR-95hsa-miR-95_st1 12.01 9.38 8.60 9.83 10.1 9.54 8.39 hsa-miR-582hsa-miR-582_st1 7.47 5.84 2.13 4.54 5.95 4.86 4.05 hsa-miR-154*hsa-miR-154-_st2 7.25 5.81 3.48 4.21 4.98 4.59 3.89 hsa-miR-204hsa-miR-204_st2 9.09 7.17 5.77 5.98 8.20 6.73 5.40 hsa-miR-433hsa-miR-433_st2 5.68 2.41 2.67 4.54 2.90 4.17 2.32 hsa-miR-218hsa-miR-218_st2 11.53 9.61 9.22 8.83 9.68 9.13 8.59 hsa-asg-14230hsa-asg-14230_st1 6.51 4.29 3.68 4.38 4.56 3.29 3.43 hsa-miR-335hsa-miR-335_st1 12.07 10.91 10.25 8.70 10.0 9.44 9.29 hsa-miR-140hsa-miR-140_st2 8.60 7.72 6.11 6.37 7.21 6.60 5.45 hsa-miR-18ahsa-miR-18a_st1 8.08 5.76 5.97 7.11 5.96 4.49 4.08 hsa-asg-9681hsa-asg-9681_st1 6.64 4.84 4.25 4.99 4.75 3.68 2.80 hsa-asg-10679hsa-asg-10679_st2 5.95 4.07 2.97 3.66 4.75 3.35 2.65 hsa-asg-14238hsa-asg-14238_st1 5.88 3.73 4.55 3.78 3.25 4.20 2.94 hsa-asg-14146hsa-asg-14146_st1 8.27 6.29 5.72 6.24 6.49 5.92 4.79 hsa-asg-11883hsa-asg-11883_st1 8.49 5.90 6.01 6.68 6.73 6.15 5.96 hsa-miR-501hsa-miR-501_st1 6.76 5.24 4.81 2.84 5.09 4.04 4.60 hsa-miR-18bhsa-miR-18b_st1 7.91 6.17 5.56 6.71 6.01 5.05 4.62 hsa-miR-594hsa-miR-594_st2 10.08 7.42 8.16 8.08 8.41 7.08 8.47 hsa-asg-14163hsa-asg-14163_st2 8.27 6.58 6.19 6.61 7.29 5.66 5.67 hsa-asg-14155hsa-asg-14155_st2 5.99 4.22 3.24 4.68 3.44 3.40 3.26 hsa-miR144hsa-miR144_st2 5.63 4.59 4.38 2.63 3.38 2.65 2.65 hsa-miR-181dhsa-miR-181d_st1 10.48 9.12 7.78 8.91 8.92 7.57 7.91 hsa-miR-181dhsa-miR74_st1 10.39 8.96 8.17 9.00 8.79 7.99 8.20 hsa-miR-181dhsa-asg-13508_st1 7.81 6.60 5.77 6.86 6.60 5.60 5.62 hsa-cand22hsa-cand22_st2 7.88 6.13 6.15 6.43 6.80 5.31 5.31 hsa-asg-12339hsa-asg-12339_st1 9.04 7.59 6.69 7.66 7.02 6.26 5.77 hsa-asg-10448hsa-asg-10448_st2 6.01 4.00 4.76 3.38 3.75 3.55 4.12 hsa-miR-181a*hsa-miR-213_st1 7.89 6.29 6.17 5.68 5.71 5.42 5.37 hsa-miR-409-5phsa-miR-409-5p_st1 7.71 5.13 4.98 6.68 6.08 6.08 5.11 hsa-asg-1221hsa-asg-1221_st2 5.60 4.00 3.24 4.38 4.21 3.73 3.08 hsa-miR-454-3phsa-asg-11541_st2 10.13 8.62 8.26 8.54 8.45 7.84 7.87 hsa-miR-454-3phsa-asg-13305_st1 10.73 9.62 8.93 9.25 9.13 8.52 8.72 hsa-miR-339hsa-miR-339_st1 7.56 6.65 5.92 5.21 6.67 5.25 5.07 hsa-miR-7hsa-miR-7_st1 10.65 9.45 8.18 9.71 8.61 8.16 7.65 hsa-asg-14150hsa-asg-14150_st1 8.38 7.04 6.30 7.01 7.73 6.64 5.75 hsa-asg-119hsa-asg-119_st1 6.77 5.24 4.94 4.93 5.11 4.38 3.43 hsa-asg-3376hsa-asg-3376_st1 5.71 3.83 2.97 4.87 4.65 3.97 4.12 hsa-miR-19bhsa-miR-19b_st2 9.32 8.37 8.28 7.32 8.02 8.01 6.45 hsa-asg-2005hsa-asg-2005_st1 6.77 4.00 3.95 5.74 4.14 5.16 5.34 hsa-miR-10ahsa-miR-10a_st1 12.93 11.99 10.95 11.21 11.7 11.3 10.4 hsa-miR-18a*hsa-miR-18a-_st1 6.71 5.66 5.32 5.53 5.44 4.76 5.00 hsa-miR-148bhsa-miR-148b_st2 10.97 10.03 8.74 9.45 10.0 9.60 8.22 hsa-asg-8476hsa-asg-8476_st1 7.84 5.58 6.36 6.43 6.32 5.75 6.17 hsa-miR-128ahsa-miR-128a_st2 11.12 10.77 9.31 9.72 9.95 8.83 8.94 hsa-asg-13912hsa-asg-13912_st1 5.93 5.09 4.18 4.68 3.98 4.07 3.81 hsa-miR257hsa-miR257_st1 10.53 9.61 9.05 9.23 9.55 8.84 8.49 hsa-asg-925hsa-asg-925_st1 8.02 7.35 6.32 6.19 6.96 6.32 5.21 hsa-miR-106bhsa-miR-106b_st2 11.16 9.90 9.59 10.02 10.0 9.40 8.12 hsa-asg-14175hsa-asg-14175_st1 7.91 6.55 6.31 6.43 7.06 6.07 6.08 hsa-asg-10401hsa-asg-10401_st1 5.99 5.09 4.98 3.90 5.03 4.20 4.49 hsa-miR-181bhsa-miR-181b_st2 12.14 11.22 10.43 10.98 10.8 9.81 10.2 hsa-asg-3046hsa-asg-3046_st2 7.16 5.13 6.50 4.93 5.84 5.09 6.22 hsa-asg-7131hsa-asg-7131_st1 8.45 6.24 7.21 6.71 7.29 6.60 7.16 hsa-miR-212hsa-miR-212_st2 6.88 6.26 5.68 6.18 5.71 5.14 5.05 hsa-asg-12429hsa-asg-12429_st2 6.77 4.84 5.33 5.82 5.18 4.88 5.48 hsa-asg-4515hsa-asg-4515_st1 6.47 5.17 4.98 4.61 5.20 5.12 5.30 hsa-miR-566hsa-miR-566_st1 7.36 6.72 6.12 5.12 5.83 6.11 6.10 hsa-miR-15ahsa-miR-15a_st1 12.46 11.57 11.16 11.16 11.3 10.7 10.6 hsa-asg-5827hsa-asg-5827_st2 6.68 5.61 4.71 5.71 5.52 5.65 5.15 hsa-asg-5134hsa-asg-5134_st2 5.99 4.93 4.50 4.87 4.35 4.94 4.25 hsa-asg-1362hsa-asg-1362_st2 5.76 5.09 4.11 4.81 5.11 4.00 3.63 hsa-asg-9115hsa-asg-9115_st1 5.39 4.59 4.25 4.11 3.98 3.73 4.54 hsa-miR-660hsa-miR-660_st1 9.92 9.28 8.94 7.94 9.15 8.60 8.38 hsa-asg-14233hsa-asg-14233_st1 10.62 9.44 9.21 9.23 10.1 9.41 8.84 hsa-asg-10298hsa-asg-10298_st1 5.45 4.53 3.78 4.38 4.21 3.93 3.97 hsa-asg-1565hsa-asg-1565_st2 5.78 4.88 4.03 4.75 5.11 4.98 4.25 hsa-asg-75hsa-asg-75_st2 6.16 5.81 5.18 4.38 5.28 4.33 4.29 hsa-asg-3084hsa-asg-3084_st1 6.70 5.05 4.85 6.09 5.58 5.09 5.18 hsa-asg-3070hsa-asg-3070_st2 6.50 5.84 5.21 4.93 4.85 5.17 5.65 hsa-asg-3854hsa-asg-3854_st1 6.59 5.05 5.50 6.14 5.74 5.02 5.52 hsa-asg-1398hsa-asg-1398_st2 6.48 5.31 5.53 5.33 5.41 4.49 4.85 hsa-asg-13298hsa-asg-13298_st1 7.01 5.17 6.20 4.87 5.96 5.40 6.18 hsa-miR-324-5phsa-miR-324-5p_st1 9.20 7.91 8.08 8.34 8.26 7.77 7.37 hsa-asg-4122hsa-asg-4122_st2 6.27 5.31 4.61 5.81 4.80 4.94 5.11 hsa-miR-222hsa-miR-222_st2 10.96 10.40 9.52 9.55 10.2 9.72 9.28 hsa-miR-510hsa-miR-510_st2 6.04 5.27 5.02 4.75 4.65 4.71 5.04 hsa-asg-5695hsa-asg-5695_st2 5.60 4.84 4.25 4.87 4.28 4.86 3.43 hsa-asg-13752hsa-asg-13752_st2 5.99 4.79 4.25 5.60 5.33 4.36 5.04 hsa-asg-4224hsa-asg-4224_st2 6.10 5.24 5.28 5.41 5.29 4.73 4.60 hsa-miR-185hsa-miR-185_st2 11.86 11.04 10.95 11.29 10.6 10.0 10.1 hsa-asg-1631hsa-asg-1631_st1 5.68 4.79 4.18 4.81 4.94 4.38 4.25 hsa-miR-223hsa-miR-223_st1 12.29 11.67 11.19 11.40 11.3 11.0 11.1 hsa-miR-22hsa-miR-22_st1 12.63 11.71 11.56 12.02 12.0 11.6 11.0 hsa-miR-362hsa-miR-362_st1 8.42 7.42 7.66 7.94 7.60 7.44 7.08 hsa-asg-6462hsa-asg-6462_st1 6.51 5.47 5.77 5.41 6.08 5.66 4.70 hsa-asg-12973hsa-asg-12973_st2 6.77 6.05 5.79 5.81 6.18 5.82 4.96 hsa-asg-10922hsa-asg-10922_st2 6.96 6.54 5.74 5.98 5.85 6.20 5.46 hsa-asg-3221hsa-asg-3221_st1 6.61 5.24 5.41 6.26 5.75 5.56 5.69 hsa-asg-11579hsa-asg-11579_st2 6.47 5.40 5.02 5.96 6.04 5.43 5.34 hsa-asg-10626hsa-asg-10626_st2 5.93 5.13 5.14 4.61 5.36 4.96 4.25 hsa-asg-12746hsa-asg-12746_st1 6.97 6.15 5.64 6.47 6.12 5.86 5.37 hsa-miR-425-5phsa-asg-14166_st2 11.31 10.97 10.56 10.57 10.4 9.80 9.85 hsa-asg-3604hsa-asg-3604_st1 6.66 5.88 5.79 5.98 5.79 5.75 5.15 LNneg MicroRNA 7 8 9M SD % ttest log2Diff Fold hsa-miR-495 5.14 2.41 4.87 5.23 1.36 565.72E−03 −5.36 41.2 hsa-miR-493-5p 4.36 1.85 4.58 4.66 1.58 33 1.37E−02−5.22 37.3 hsa-asg-13404 2.14 1.09 2.29 2.82 1.29 0 5.46E−03 −5.13 34.9hsa-miR-493-3p 1.96 1.56 1.71 1.40 0.73 0 2.07E−04 −4.91 30.2hsa-miR-493-3p 4.31 3.46 4.08 3.41 0.95 0 1.22E−02 −3.24 9.5 hsa-miR-3384.76 3.40 4.87 4.30 1.04 11 2.39E−03 −4.80 27.9 hsa-miR-368 5.62 3.975.07 6.25 1.40 67 1.26E−02 −4.73 26.6 hsa-miR202 4.25 2.41 3.06 3.450.88 0 9.77E−04 −4.68 25.6 hsa-miR-487b 4.88 3.71 4.37 5.58 1.11 674.58E−03 −4.56 23.6 hsa-asg-13166 3.57 2.05 4.04 3.94 0.88 0 1.24E−03−4.54 23.3 hsa-miR-139 9.34 7.34 5.90 8.84 1.48 100 2.08E−02 −4.48 22.3hsa-miR-376a 2.47 3.80 3.73 4.34 1.02 0 3.50E−03 −4.39 21.0 hsa-miR-376a2.90 4.04 1.83 3.57 0.86 0 1.67E−03 −4.23 18.8 hsa-miR-411 3.38 2.232.07 3.83 1.27 11 1.32E−02 −4.26 19.1 hsa-miR-539 1.96 2.58 1.36 2.520.63 0 2.40E−04 −4.18 18.1 hsa-miR-539 3.48 1.66 2.07 2.19 0.63 02.42E−04 −4.16 17.9 hsa-miR-376a 3.83 4.12 4.64 4.83 0.75 11 7.73E−04−4.16 17.9 hsa-asg-5021 4.57 3.03 2.07 4.63 1.58 22 3.72E−02 −4.16 17.8hsa-miR-432 3.48 4.25 2.71 4.80 1.13 22 8.36E−03 −4.14 17.6 hsa-miR-4245.80 5.70 7.21 5.69 0.98 89 4.07E−03 −4.11 17.3 hsa-asg-5617 1.42 1.461.60 2.78 1.03 0 5.29E−03 −4.10 17.2 hsa-miR-409-3p 3.95 2.58 3.73 3.751.06 0 7.08E−03 −4.00 16.0 hsa-miR-369-5p 3.38 2.74 3.50 3.36 0.56 01.54E−04 −3.96 15.5 hsa-miR-369-5p 2.90 2.58 3.50 3.42 0.81 0 2.36E−03−3.72 13.1 hsa-asg-13254 4.71 2.41 3.36 4.86 1.37 56 2.57E−02 −3.96 15.5hsa-miR-381 3.48 1.28 3.22 3.08 0.91 0 3.70E−03 −3.90 14.9 hsa-miR-4504.18 4.44 4.04 3.76 0.62 0 3.29E−04 −3.90 14.9 hsa-miR-450 2.90 2.892.71 2.79 0.42 0 2.64E−05 −3.75 13.5 hsa-cand26 5.27 4.04 5.44 4.98 0.9122 3.63E−03 −3.89 14.9 hsa-miR-127 6.57 5.47 5.42 6.51 1.03 56 7.92E−03−3.82 14.2 hsa-asg-10881 4.18 5.01 5.20 5.19 0.55 22 1.80E−04 −3.79 13.8hsa-asg-13135 5.14 4.44 5.57 5.11 0.44 22 3.78E−05 −3.76 13.5hsa-miR-410 3.15 1.85 2.51 2.51 0.68 0 7.96E−04 −3.76 13.5hsa-miR-485-3p 3.57 2.74 3.06 3.24 0.66 0 7.24E−04 −3.69 12.9hsa-miR-485-3p 3.66 3.51 3.84 3.74 0.51 0 5.52E−04 −3.00 8.0hsa-asg-7472 3.57 0.92 0.93 1.97 0.85 0 3.44E−03 −3.66 12.7 hsa-miR-487a0.93 2.23 2.89 2.50 0.73 0 1.51E−03 −3.64 12.5 hsa-miR-487a 3.66 3.713.62 3.72 0.20 0 3.41E−06 −2.39 5.3 hsa-miR-376b 2.77 2.50 0.22 2.281.17 0 1.99E−02 −3.56 11.8 hsa-cand206 3.57 1.28 2.71 2.65 0.86 04.47E−03 −3.54 11.6 hsa-miR-154 3.15 1.28 3.94 3.47 1.10 0 1.95E−02−3.39 10.5 hsa-miR-542-5p 3.83 4.66 5.16 3.81 0.76 0 2.87E−03 −3.38 10.4hsa-miR-542-3p 5.38 5.88 4.70 5.55 0.49 78 1.95E−04 −3.37 10.3hsa-asg-8067 5.30 5.28 5.38 5.97 0.60 33 7.22E−04 −3.35 10.2 hsa-miR-1344.71 4.29 5.00 5.16 0.68 0 1.71E−03 −3.33 10.1 hsa-miR-379 6.33 6.136.20 6.58 0.37 100 3.12E−05 −3.25 9.5 hsa-miR-329 2.77 3.29 2.71 3.410.74 0 3.38E−03 −3.19 9.1 hsa-miR-494 5.71 5.84 6.58 6.68 0.64 1001.55E−03 −3.18 9.0 hsa-miR-494 5.38 5.77 6.87 6.45 0.55 89 1.64E−03−2.71 6.5 hsa-asg-9696 6.61 6.53 5.07 6.46 0.65 89 1.78E−03 −3.16 8.9hsa-miR-503 6.39 6.60 5.99 6.62 0.46 78 2.03E−04 −3.15 8.9 hsa-miR-5036.85 6.87 6.74 6.87 0.30 100 2.17E−05 −2.79 6.9 hsa-miR-329 3.66 2.743.06 3.16 0.84 0 7.80E−03 −3.13 8.8 hsa-miR-329 3.98 2.58 3.50 3.58 0.590 1.10E−03 −3.11 8.6 hsa-miR-382 6.27 5.91 5.79 6.70 0.61 78 1.44E−03−3.07 8.4 hsa-miR-95 9.08 7.80 8.19 9.00 0.80 100 7.21E−03 −3.01 8.0hsa-miR-582 4.05 4.32 4.64 4.49 1.12 22 3.59E−02 −2.98 7.9 hsa-miR-154*4.25 3.17 4.37 4.31 0.79 11 7.61E−03 −2.95 7.7 hsa-miR-204 5.17 5.405.76 6.18 1.00 44 2.43E−02 −2.92 7.6 hsa-miR-433 1.60 1.66 2.89 2.801.00 0 2.60E−02 −2.89 7.4 hsa-miR-218 7.75 7.43 8.74 8.77 0.77 1009.32E−03 −2.76 6.8 hsa-asg-14230 2.77 3.93 4.04 3.82 0.58 0 2.33E−03−2.69 6.4 hsa-miR-335 9.57 7.32 9.01 9.40 1.03 100 3.93E−02 −2.67 6.4hsa-miR-140 4.76 4.75 5.24 6.02 1.05 44 4.91E−02 −2.58 6.0 hsa-miR-18a5.67 5.65 4.87 5.51 0.91 44 2.72E−02 −2.57 6.0 hsa-asg-9681 4.05 3.294.51 4.13 0.75 0 1.27E−02 −2.51 5.7 hsa-asg-10679 3.15 3.29 3.06 3.440.64 0 5.81E−03 −2.51 5.7 hsa-asg-14238 3.83 1.46 2.71 3.39 0.93 03.40E−02 −2.49 5.6 hsa-asg-14146 5.78 4.66 6.46 5.82 0.68 78 8.92E−03−2.46 5.5 hsa-asg-11883 6.11 4.55 6.42 6.06 0.64 78 7.01E−03 −2.43 5.4hsa-miR-501 4.47 4.25 4.37 4.41 0.70 0 1.32E−02 −2.35 5.1 hsa-miR-18b5.69 4.85 5.57 5.58 0.67 44 1.07E−02 −2.33 5.0 hsa-miR-594 8.44 7.716.09 7.76 0.79 67 2.39E−02 −2.32 5.0 hsa-asg-14163 6.29 4.89 4.37 5.950.91 78 4.15E−02 −2.32 5.0 hsa-asg-14155 3.75 3.80 3.36 3.68 0.49 02.06E−03 −2.31 5.0 hsa-miR144 2.77 3.17 4.21 3.38 0.81 0 2.94E−02 −2.254.7 hsa-miR-181d 8.26 8.20 8.00 8.30 0.56 100 5.94E−03 −2.19 4.6hsa-miR-181d 8.08 8.15 7.86 8.36 0.44 100 2.20E−03 −2.04 4.1hsa-miR-181d 5.67 5.79 5.51 6.00 0.52 67 1.13E−02 −1.81 3.5 hsa-cand225.80 5.65 3.73 5.70 0.89 78 4.81E−02 −2.18 4.5 hsa-asg-12339 6.82 7.426.59 6.87 0.63 89 1.12E−02 −2.17 4.5 hsa-asg-10448 3.38 4.25 4.04 3.910.45 0 2.26E−03 −2.10 4.3 hsa-miR-181a* 5.30 5.77 6.53 5.80 0.43 561.86E−03 −2.09 4.2 hsa-miR-409-5p 5.90 5.43 5.24 5.63 0.58 44 9.34E−03−2.09 4.2 hsa-asg-1221 2.62 2.89 3.50 3.52 0.61 0 1.17E−02 −2.08 4.2hsa-miR-454-3p 7.89 7.31 7.80 8.06 0.43 100 1.86E−03 −2.07 4.2hsa-miR-454-3p 8.55 7.80 8.31 8.76 0.54 100 8.84E−03 −1.97 3.9hsa-miR-339 5.00 4.22 5.42 5.49 0.80 33 3.93E−02 −2.07 4.2 hsa-miR-78.64 8.56 8.46 8.60 0.64 100 1.58E−02 −2.05 4.1 hsa-asg-14150 5.77 5.136.37 6.41 0.80 11 4.74E−02 −1.96 3.9 hsa-asg-119 4.84 5.43 5.51 4.870.63 44 2.15E−02 −1.91 3.7 hsa-asg-3376 2.47 4.19 3.36 3.82 0.77 04.97E−02 −1.88 3.7 hsa-miR-19b 6.90 6.51 7.18 7.45 0.74 100 4.42E−02−1.87 3.7 hsa-asg-2005 5.07 5.30 5.64 4.93 0.71 44 3.82E−02 −1.85 3.6hsa-miR-10a 11.2 9.96 10.8 11.0 0.62 100 2.18E−02 −1.85 3.6 hsa-miR-18a*3.66 4.12 4.29 4.86 0.70 11 3.79E−02 −1.85 3.6 hsa-miR-148b 9.07 8.129.12 9.16 0.71 100 4.17E−02 −1.81 3.5 hsa-asg-8476 6.75 5.40 5.61 6.040.47 11 6.51E−03 −1.80 3.5 hsa-miR-128a 9.00 8.75 8.66 9.33 0.70 1004.06E−02 −1.79 3.5 hsa-asg-13912 3.98 4.19 3.73 4.19 0.43 11 5.25E−03−1.74 3.3 hsa-miR257 8.48 8.13 7.89 8.81 0.61 100 2.74E−02 −1.72 3.3hsa-asg-925 5.80 5.72 6.86 6.31 0.67 89 4.20E−02 −1.72 3.3 hsa-miR-106b9.19 9.08 9.75 9.46 0.61 100 2.88E−02 −1.71 3.3 hsa-asg-14175 5.57 5.916.23 6.25 0.42 89 5.65E−03 −1.67 3.2 hsa-asg-10401 4.18 4.04 3.06 4.330.65 22 4.27E−02 −1.66 3.2 hsa-miR-181b 10.2 10.4 10.1 10.4 0.45 1008.24E−03 −1.65 3.1 hsa-asg-3046 5.64 5.36 5.84 5.62 0.54 78 2.58E−02−1.54 2.9 hsa-asg-7131 6.28 7.49 7.39 6.93 0.48 33 1.70E−02 −1.52 2.9hsa-miR-212 4.57 4.95 4.73 5.36 0.61 11 4.78E−02 −1.51 2.9 hsa-asg-124295.10 5.28 5.38 5.26 0.30 0 1.48E−03 −1.51 2.9 hsa-asg-4515 4.71 4.665.11 4.99 0.26 0 6.14E−04 −1.48 2.8 hsa-miR-566 5.55 6.02 5.42 5.89 0.4756 1.81E−02 −1.48 2.8 hsa-miR-15a 10.6 10.5 11.0 10.9 0.36 100 4.51E−03−1.47 2.8 hsa-asg-5827 4.76 5.16 4.70 5.22 0.42 22 1.08E−02 −1.47 2.8hsa-asg-5134 4.80 3.80 4.44 4.54 0.38 0 6.94E−03 −1.45 2.7 hsa-asg-13623.75 4.04 4.29 4.31 0.56 11 3.91E−02 −1.45 2.7 hsa-asg-9115 3.57 3.403.50 3.96 0.44 0 1.59E−02 −1.42 2.7 hsa-miR-660 7.78 7.90 8.59 8.51 0.55100 4.13E−02 −1.42 2.7 hsa-asg-14233 9.03 8.85 8.87 9.23 0.41 1001.30E−02 −1.39 2.6 hsa-asg-10298 4.25 3.71 3.84 4.07 0.29 0 1.82E−03−1.38 2.6 hsa-asg-1565 3.57 4.12 4.21 4.43 0.52 0 3.81E−02 −1.35 2.6hsa-asg-75 5.03 4.55 4.44 4.81 0.54 11 4.43E−02 −1.35 2.5 hsa-asg-30845.44 5.48 5.40 5.35 0.36 56 8.07E−03 −1.34 2.5 hsa-asg-3070 5.36 4.614.87 5.16 0.40 22 1.33E−02 −1.33 2.5 hsa-asg-3854 4.52 5.23 4.76 5.280.50 56 3.87E−02 −1.31 2.5 hsa-asg-1398 4.84 5.43 5.57 5.20 0.37 111.15E−02 −1.29 2.4 hsa-asg-13298 6.00 5.48 6.20 5.72 0.50 44 4.05E−02−1.29 2.4 hsa-miR-324-5p 7.80 7.85 7.97 7.93 0.29 100 3.05E−03 −1.27 2.4hsa-asg-4122 5.03 4.71 4.82 5.01 0.37 33 1.18E−02 −1.25 2.4 hsa-miR-2229.50 9.37 9.84 9.71 0.38 100 1.45E−02 −1.25 2.4 hsa-miR-510 4.52 4.255.07 4.81 0.32 11 6.41E−03 −1.23 2.3 hsa-asg-5695 4.67 4.04 4.21 4.380.48 0 4.26E−02 −1.22 2.3 hsa-asg-13752 4.52 4.66 4.58 4.79 0.45 03.55E−02 −1.20 2.3 hsa-asg-4224 4.71 4.61 4.37 4.92 0.39 22 2.00E−02−1.18 2.3 hsa-miR-185 10.4 11.2 10.2 10.6 0.47 100 4.51E−02 −1.18 2.3hsa-asg-1631 4.42 4.50 4.64 4.55 0.26 0 3.55E−03 −1.14 2.2 hsa-miR-22311.0 10.6 11.0 11.1 0.30 100 7.92E−03 −1.13 2.2 hsa-miR-22 11.1 11.211.1 11.5 0.39 100 2.55E−02 −1.13 2.2 hsa-miR-362 6.93 6.55 7.04 7.300.43 100 3.75E−02 −1.13 2.2 hsa-asg-6462 5.20 4.80 5.40 5.39 0.44 444.21E−02 −1.13 2.2 hsa-asg-12973 5.03 5.60 5.70 5.66 0.41 78 3.41E−02−1.11 2.2 hsa-asg-10922 5.80 5.75 5.40 5.86 0.35 56 1.78E−02 −1.10 2.1hsa-asg-3221 5.24 5.33 5.16 5.52 0.35 0 1.72E−02 −1.10 2.1 hsa-asg-115795.07 4.80 5.28 5.37 0.41 67 3.47E−02 −1.09 2.1 hsa-asg-10626 4.84 4.254.97 4.84 0.39 0 2.90E−02 −1.09 2.1 hsa-asg-12746 6.05 5.54 5.87 5.900.34 100 1.77E−02 −1.08 2.1 hsa-miR-425-5p 10.1 9.93 9.87 10.2 0.41 1003.91E−02 −1.07 2.1 hsa-asg-3604 5.71 5.30 5.28 5.63 0.30 78 1.18E−02−1.03 2.0 SD, standard deviation; %, percentage of samples expressingmiRNA above background levels; Log2Diff, difference in VSN-transformedexpression bewteen SPL and LNneg samples.

Example 33 miRNAs Differentally Expressed Between Normal Tongue Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous tongue cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal tonguetissue than in cancer-negative lymph nodes.

Total RNA, purified from one normal human tongue sample (FirstChoice®Human Tongue Total RNA, cat. no. B7800-2, Ambion, Austin, Tex., USA) andfrom nine normal human lymph node samples (FirstChoice® Human Lymph NodeTotal RNA, cat. no. AM7894; Ambion, Austin, Tex., USA) was purchasedfrom Ambion. miRNA purification and labeling and microarray expressionanalyses were performed as described above in Example 2.

The inventors identified 158 miRNAs (62 verified, 96 predicted) withsignificant differential expression between thenormal tongue and normallymph node sample (|log 2Diff| tongue vs LN neg≧1 and p-value<0.05)(Table 32). Among the verified miRNAs, hsa-miR-206, -205 and -375 wereexpressed at levels between 150- and 400-fold higher in the tonguesample; 9 miRNAs (hsa-miR-381, -499, -133b, -203, -429, -299-5p, -411,-133a and -493-5p) were expressed at levels between 50- and 100-foldhigher in tongue; 9 miRNAs were expressed at levels between 20- and50-fold higher in tongue compared to LNneg; 11 miRNAs were expressed atlevels between 10- and 20-fold higher in tongue; and 22 miRNAs wereexpressed at levels between 5- and 10-fold higher in the tongue samplethan in the lymph node samples. Among the predicted miRNAs, 9 miRNAs(hsa-asg-2027_st1, -14153_st2, -5617_st1, -2345_st2, -3376_st1,-7637_st2, -1794_st1, -5021_st1, and hsa-cand14_st1) were expressed atlevels between 20- and 50-fold higher in tongue; 24 miRNAs wereexpressed at levels between 10- and 20-fold higher in the tongue samplecompared to normal lymph nodes; and 30 miRNAs were expressed at levelsbetween 5- and 10-fold higher in the tongue sample than in normal lymphnodes.

TABLE 32 MicroRNAs Differentially Expressed Between Normal TongueSamples (TNG) and Cancer-Negative Lymph Node Samples (LNneg) fromPatients. Probe LNneg MicroRNA ID TNG 1 2 3 4 5 6 hsa-miR-206hsa-miR-206_st2 13.91 4.07 4.94 1.89 9.39 3.93 5.79 hsa-miR-205hsa-miR-205_st1 13.17 2.90 5.86 5.64 2.00 3.55 6.06 hsa-miR-375hsa-miR-375_st1 11.90 5.95 4.88 6.24 2.82 2.93 3.63 hsa-miR-381hsa-miR-381_st2 9.71 2.75 2.32 4.21 3.38 4.17 2.94 hsa-miR-499hsa-miR12_st1 11.50 5.44 4.76 5.37 6.66 4.07 4.38 hsa-miR-499hsa-miR-499_st1 11.40 5.31 4.61 4.75 6.57 4.49 4.12 hsa-miR-133bhsa-miR-133b_st2 13.07 7.35 6.17 4.46 10.19 7.42 6.15 hsa-miR-203hsa-miR-203_st2 13.23 7.32 5.84 7.22 8.24 6.42 5.36 hsa-miR-429hsa-miR-429_st1 9.16 3.42 1.54 3.66 2.10 2.33 2.65 hsa-miR-299-5phsa-miR-299-5p_st2 10.94 5.71 4.44 5.81 6.37 5.96 2.94 hsa-miR-411hsa-miR-411_st1 9.95 4.84 3.68 4.54 5.22 5.52 2.94 hsa-miR-133ahsa-miR-133a_st1 13.48 7.76 7.65 5.93 10.81 8.16 7.08 hsa-miR-493-5phsa-miR-493-5p_st1 10.32 4.35 3.78 6.89 6.18 6.30 3.63 hsa-asg-2027hsa-asg-2027_st1 9.44 3.53 3.48 1.63 5.22 4.44 4.19 hsa-miR-200ahsa-miR-200a_st2 11.04 6.26 5.02 6.49 5.71 5.25 4.54 hsa-miR-200bhsa-miR-200b_st1 13.05 7.61 7.75 8.91 6.69 6.79 6.82 hsa-asg-14153hsa-asg-14153_st2 7.99 3.05 2.23 3.04 2.98 2.33 2.65 hsa-miR-539hsa-miR177_st1 7.42 2.58 2.50 1.37 2.39 1.80 1.86 hsa-miR-539hsa-miR-539_st1 7.62 2.03 3.11 2.63 3.38 2.65 2.94 hsa-miR-378hsa-miR-378_st1 9.24 4.69 4.41 4.84 6.38 5.43 2.14 hsa-miR-368hsa-miR-368_st2 11.08 6.48 6.47 7.70 8.08 7.68 5.18 hsa-asg-5617hsa-asg-5617_st1 7.57 3.73 3.24 3.90 3.60 3.40 2.65 hsa-miR-495hsa-miR-495_st1 9.90 6.01 4.76 6.97 6.25 6.29 4.38 hsa-miR-200chsa-miR-200c_st1 13.61 9.59 8.79 10.3 8.17 8.12 8.08 hsa-asg-2345hsa-asg-2345_st2 6.91 1.63 2.50 2.40 3.60 1.44 2.49 hsa-asg-3376hsa-asg-3376_st1 8.36 3.83 2.97 4.87 4.65 3.97 4.12 hsa-miR-376ahsa-miR-376a_st2 8.83 5.13 4.03 5.49 5.66 4.82 3.97 hsa-miR-376ahsa-asg-14234_st1 7.88 3.31 4.25 3.38 3.98 4.82 3.63 hsa-miR-376ahsa-asg-10049_st1 9.05 5.01 4.85 6.09 5.55 5.31 4.05 hsa-asg-7637hsa-asg-7637_st2 8.24 3.64 2.50 4.46 3.44 3.89 3.97 hsa-miR-576hsa-miR-576_st2 6.74 2.22 2.83 3.04 1.69 1.98 1.95 hsa-miR-487bhsa-miR-487b_st2 10.01 6.05 5.79 6.97 6.90 6.22 5.34 hsa-cand14hsa-cand14_st1 6.63 1.83 1.54 1.37 2.10 2.79 1.95 hsa-asg-1794hsa-asg-1794_st1 6.35 1.83 1.94 2.63 1.69 2.16 1.03 hsa-asg-5021hsa-asg-5021_st1 8.96 5.17 4.38 7.00 6.05 5.88 3.48 hsa-asg-9198hsa-asg-9198_st1 5.85 2.03 1.54 1.37 1.09 2.07 1.76 hsa-cand206hsa-cand206_st1 6.87 2.22 1.94 3.78 3.60 2.65 2.14 hsa-asg-2399hsa-asg-2399_st2 6.71 3.05 3.36 2.84 2.98 2.16 2.49 hsa-asg-7472hsa-asg-7472_st2 6.14 1.63 2.13 1.89 2.00 2.86 1.76 hsa-miR-141hsa-miR-141_st1 8.31 5.05 3.78 4.93 4.41 4.36 2.80 hsa-asg-11715hsa-asg-11715_st2 6.03 1.83 2.32 2.15 1.48 2.07 1.76 hsa-miR-605hsa-miR-605_st1 6.64 2.58 2.13 3.04 0.73 1.71 2.14 hsa-asg-8428hsa-asg-8428_st2 5.90 2.22 1.94 2.63 2.00 2.49 1.03 hsa-asg-14065hsa-asg-14065_st2 6.84 1.24 2.58 5.31 4.62 1.80 2.49 hsa-asg-13254hsa-asg-13254_st1 8.91 5.58 5.32 6.66 5.89 5.87 3.97 hsa-asg-791hsa-asg-791_st1 6.00 2.22 2.97 1.63 2.00 2.16 2.40 hsa-miR-379hsa-miR-379_st1 10.58 6.51 6.37 7.12 6.96 7.01 6.54 hsa-asg-2484hsa-asg-2484_st2 6.46 2.41 2.32 1.37 3.98 2.16 2.32 hsa-miR-127hsa-miR-127_st1 10.35 6.80 5.52 7.96 7.53 7.66 5.66 hsa-asg-13404hsa-asg-13404_st2 6.53 4.15 2.67 5.28 2.90 3.24 1.57 hsa-asg-1826hsa-asg-1826_st2 6.38 3.31 2.97 2.15 3.55 2.49 2.14 hsa-miR-517chsa-miR-517c_st2 6.32 2.90 2.50 3.04 3.60 3.06 3.43 hsa-asg-1296hsa-asg-1296_st1 6.85 3.05 2.50 4.81 2.39 2.49 2.73 hsa-miR-34chsa-miR-34c_st2 7.18 1.83 2.50 3.52 3.66 3.59 3.01 hsa-miR-376bhsa-miR-376b_st1 5.86 1.83 2.13 4.11 3.32 2.65 1.03 hsa-asg-11777hsa-asg-11777_st1 6.06 2.58 2.13 1.89 3.60 1.98 1.95 hsa-miR-369-5phsa-asg-1521_st1 6.94 3.73 3.36 4.01 2.20 3.68 3.68 hsa-miR-369-5phsa-miR-369-5p_st2 6.55 3.53 2.67 4.46 4.35 4.33 2.49 hsa-asg-10250hsa-asg-10250_st1 5.61 1.83 2.13 1.12 2.10 2.79 1.76 hsa-asg-12139hsa-asg-12139_st2 5.88 2.75 2.32 2.63 2.30 1.62 2.14 hsa-miR-154hsa-miR-154_st2 7.01 3.73 2.50 4.30 4.35 4.88 3.08 hsa-asg-10131hsa-asg-10131_st2 6.76 3.31 3.11 3.52 2.48 2.49 3.20 hsa-asg-128hsa-asg-128_st2 8.08 4.84 4.71 4.54 5.11 4.52 4.12 hsa-asg-13705hsa-asg-13705_st1 5.75 1.63 0.97 3.78 2.30 2.65 2.32 hsa-asg-10393hsa-asg-10393_st1 6.13 2.75 1.54 2.40 3.89 2.79 2.80 hsa-asg-11088hsa-asg-11088_st1 5.74 1.24 1.06 3.84 1.79 0.94 2.32 hsa-asg-1765hsa-asg-1765_st1 5.96 2.22 2.13 2.84 3.60 2.33 2.32 hsa-asg-924hsa-asg-924_st1 6.77 4.64 4.25 3.38 4.44 2.65 2.73 hsa-miR-187hsa-miR-187_st1 6.72 4.74 2.75 2.74 3.75 3.18 3.32 hsa-asg-12142hsa-asg-12142_st1 5.66 2.58 2.67 2.15 3.50 1.80 1.76 hsa-miR-517ahsa-miR-517b_st1 7.54 4.97 5.56 6.51 3.85 3.18 3.77 hsa-asg-6178hsa-asg-6178_st2 5.56 2.41 3.36 2.40 3.80 2.16 1.21 hsa-miR-489hsa-miR-489_st2 9.45 7.34 6.75 6.01 7.47 6.33 5.45 hsa-asg-13166hsa-asg-13166_st2 7.21 4.47 3.36 4.87 4.94 4.07 4.12 hsa-asg-5119hsa-asg-5119_st1 5.73 2.41 2.67 1.89 1.90 1.98 2.49 hsa-miR202hsa-miR202_st2 6.63 3.83 2.67 4.93 3.94 3.59 2.40 hsa-asg-13231hsa-asg-13231_st1 6.63 3.91 4.11 4.21 4.44 2.93 3.38 hsa-asg-3998hsa-asg-3998_st1 11.90 8.62 8.53 8.70 9.41 8.74 8.32 hsa-asg-1619hsa-asg-1619_st2 6.45 4.35 3.24 2.84 4.80 3.12 2.80 hsa-miR-432hsa-miR-432_st2 7.92 4.53 5.38 5.93 5.52 5.74 5.62 hsa-miR-493-3phsa-miR-493-3p_st1 6.51 4.64 3.05 4.06 2.98 2.24 1.86 hsa-asg-54hsa-asg-54_st2 5.23 2.22 0.97 2.15 2.48 1.35 2.80 hsa-miR-329hsa-asg-13589_st2 6.21 1.63 3.11 3.78 3.71 4.38 2.32 hsa-miR-329hsa-miR-329_st1 5.80 3.73 2.97 4.61 3.25 4.54 2.80 hsa-miR-329hsa-asg-13204_st2 5.04 4.07 2.83 4.21 3.38 4.20 3.43 hsa-miR-410hsa-miR-410_st1 5.56 2.41 2.32 1.37 3.71 2.49 2.80 hsa-asg-378hsa-asg-378_st1 5.75 2.22 2.67 2.15 4.70 2.79 2.94 hsa-asg-13878hsa-asg-13878_st2 6.21 2.90 3.48 2.84 4.82 4.24 3.32 hsa-asg-13070hsa-asg-13070_st1 5.51 2.90 2.50 2.63 4.14 3.06 2.14 hsa-miR-128ahsa-miR-128a_st2 12.26 10.7 9.31 9.72 9.95 8.83 8.94 hsa-miR-422ahsa-miR-422a_st1 12.14 9.09 8.97 9.17 9.80 8.95 8.80 hsa-asg-3822hsa-asg-3822_st2 5.65 2.03 2.97 3.66 2.00 2.41 2.14 hsa-miR-189hsa-miR-189_st1 8.24 6.07 5.74 4.93 7.23 6.15 4.49 hsa-miR-200a*hsa-miR-200a-_st2 5.62 3.05 2.83 2.15 3.89 2.57 1.76 hsa-asg-1203hsa-asg-1203_st1 6.59 4.47 4.31 2.63 3.80 4.17 4.49 hsa-asg-4439hsa-asg-4439_st2 6.20 3.83 4.31 3.21 2.66 1.62 3.63 hsa-miR-299-3phsa-asg-10378_st1 5.62 3.64 3.36 3.21 1.90 2.41 1.95 hsa-miR-31hsa-miR-31_st2 12.04 10.4 9.74 8.95 10.15 8.68 8.54 hsa-miR-338hsa-miR-338_st1 7.12 5.09 4.03 5.57 4.85 4.00 2.14 hsa-asg-9365hsa-asg-9365_st2 5.25 3.31 2.13 1.89 2.48 2.49 2.32 hsa-miR-128bhsa-miR-128b_st1 12.52 11.2 9.78 10.0 10.35 9.17 9.27 hsa-asg-9723hsa-asg-9723_st2 5.21 2.58 2.83 2.84 2.57 1.10 2.14 hsa-asg-2844hsa-asg-2844_st1 5.61 3.73 4.18 2.15 2.82 1.98 2.94 hsa-miR-582hsa-miR-582_st1 7.23 5.84 2.13 4.54 5.95 4.86 4.05 hsa-miR-95hsa-miR-95_st1 11.71 9.38 8.60 9.83 10.16 9.54 8.39 hsa-miR-154*hsa-miR-154-_st2 7.01 5.81 3.48 4.21 4.98 4.59 3.89 hsa-asg-13997hsa-asg-13997_st2 5.21 3.05 3.24 2.15 2.98 2.16 2.32 hsa-asg-11160hsa-asg-11160_st2 5.89 2.75 3.11 3.38 3.05 2.86 2.65 hsa-asg-10947hsa-asg-10947_st1 5.99 3.64 3.48 1.63 5.09 3.73 3.63 hsa-asg-7159hsa-asg-7159_st2 6.01 3.53 3.24 2.40 3.94 3.89 3.43 hsa-miR-96hsa-miR-96_st1 5.90 4.97 3.36 3.04 2.98 4.07 2.80 hsa-asg-1587hsa-asg-1587_st1 5.08 2.22 1.94 3.04 1.48 3.06 2.32 hsa-asg-8174hsa-asg-8174_st1 5.85 3.31 4.03 2.84 2.98 2.86 3.20 hsa-miR-422bhsa-miR-422b_st2 13.35 10.6 10.5 10.7 11.31 10.68 10.34 hsa-asg-1270hsa-asg-1270_st2 6.23 4.22 3.58 1.89 4.06 4.54 4.83 hsa-asg-10360hsa-asg-10360_st1 5.82 3.42 3.36 3.04 4.65 3.68 3.20 hsa-cand207hsa-cand207_st1 5.67 2.58 4.11 3.66 2.48 3.64 3.63 hsa-asg-8616hsa-asg-8616_st2 5.89 3.42 4.44 1.63 4.53 3.55 4.19 hsa-miR-382hsa-miR-382_st1 9.15 7.16 6.80 7.35 7.13 7.40 6.51 hsa-cand330hsa-cand330_st2 6.58 4.74 4.98 3.52 4.70 5.12 4.12 hsa-asg-4283hsa-asg-4283_st2 5.57 3.91 3.48 2.63 3.80 3.89 2.80 hsa-miR-9*hsa-miR-9-_st2 7.88 6.19 6.05 5.57 6.10 5.37 5.81 hsa-asg-13471hsa-asg-13471_st1 6.94 4.74 4.71 4.38 5.11 5.31 5.34 hsa-miR-183hsa-miR-183_st1 9.28 7.65 6.71 6.71 7.93 7.54 5.78 hsa-asg-4840hsa-asg-4840_st1 7.09 5.44 5.02 3.52 5.28 5.40 4.98 hsa-asg-11361hsa-asg-11361_st2 5.66 2.75 3.48 3.38 3.25 3.59 3.32 hsa-asg-1596hsa-asg-1596_st2 6.45 4.41 5.06 4.01 3.19 4.36 4.12 hsa-asg-13888hsa-asg-13888_st2 4.47 1.43 2.83 1.63 3.12 1.80 2.14 hsa-asg-11883hsa-asg-11883_st1 8.28 5.90 6.01 6.68 6.73 6.15 5.96 hsa-miR-9hsa-miR-9_st2 5.48 3.42 3.48 2.84 3.25 3.59 2.14 hsa-asg-12515hsa-asg-12515_st2 6.16 4.00 4.25 3.04 4.10 4.57 4.32 hsa-asg-10236hsa-asg-10236_st2 5.97 4.07 3.48 3.04 4.51 5.14 3.72 hsa-asg-13135hsa-asg-13135_st1 7.28 5.61 5.61 5.24 4.89 4.76 4.70 hsa-asg-3251hsa-asg-3251_st1 5.64 4.22 4.11 4.01 2.82 3.50 3.89 hsa-asg-11923hsa-asg-11923_st1 5.61 4.15 3.86 3.38 4.28 4.20 3.43 hsa-asg-4140hsa-asg-4140_st2 5.66 3.42 3.48 3.21 4.35 3.77 3.43 hsa-asg-2419hsa-asg-2419_st2 4.57 2.58 2.67 3.38 2.82 1.98 1.76 hsa-asg-67hsa-asg-67_st2 5.56 4.53 3.24 4.01 2.30 3.18 3.53 hsa-miR-494hsa-miR-494_st1 8.70 6.39 7.25 7.23 6.95 6.60 7.61 hsa-miR-409-5phsa-miR-409-5p_st1 7.64 5.13 4.98 6.68 6.08 6.08 5.11 hsa-cand443hsa-cand443_st2 6.55 3.91 5.10 3.90 4.48 5.73 5.21 hsa-asg-9514hsa-asg-9514_st1 5.34 2.58 4.50 3.21 2.48 4.33 3.63 hsa-miR-135bhsa-cand78_st1 5.08 3.42 2.50 3.66 4.14 3.68 2.80 hsa-asg-10478hsa-asg-10478_st2 5.28 3.05 4.03 3.38 2.48 4.38 4.12 hsa-asg-10679hsa-asg-10679_st2 5.29 4.07 2.97 3.66 4.75 3.35 2.65 hsa-asg-10881hsa-asg-10881_st1 7.02 5.71 4.98 6.06 5.43 4.76 5.40 hsa-miR-134hsa-miR-134_st2 6.85 5.09 5.73 6.00 5.84 5.67 4.15 hsa-miR-486hsa-miR-486_st2 10.66 8.64 9.11 8.28 9.94 9.23 8.87 hsa-asg-11397hsa-asg-11397_st1 5.28 3.64 4.25 3.90 3.50 3.93 3.63 hsa-asg-3742hsa-asg-3742_st1 5.60 3.83 4.90 3.66 4.25 4.84 3.43 hsa-asg-6803hsa-asg-6803_st1 5.56 5.01 3.36 3.66 4.48 4.59 4.12 hsa-asg-9009hsa-asg-9009_st1 6.11 5.01 5.52 4.16 4.48 4.41 5.20 hsa-asg-14233hsa-asg-14233_st1 10.70 9.44 9.21 9.23 10.14 9.41 8.84 hsa-asg-807hsa-asg-807_st2 6.30 4.15 5.41 4.54 4.35 4.71 5.52 hsa-asg-13279hsa-asg-13279_st1 8.60 7.57 7.42 7.55 8.17 7.21 6.54 hsa-asg-5149hsa-asg-5149_st2 6.21 5.31 4.92 4.71 4.82 5.40 5.21 hsa-asg-11181hsa-asg-11181_st1 6.89 5.66 5.19 6.13 6.30 5.56 5.54 hsa-asg-7471hsa-asg-7471_st1 6.96 5.58 5.67 5.68 6.74 5.67 5.25 hsa-asg-8476hsa-asg-8476_st1 7.35 5.58 6.36 6.43 6.32 5.75 6.17 hsa-miR-628hsa-miR-628_st2 8.90 8.29 7.83 7.21 8.00 7.32 7.47 hsa-miR-210hsa-miR-210_st1 7.89 6.50 5.60 6.85 7.08 7.08 6.66 hsa-asg-4438hsa-asg-4438_st2 5.87 4.53 4.50 5.04 4.53 4.11 4.49 hsa-asg-12179hsa-asg-12179_st1 6.45 6.15 4.98 5.45 5.67 5.36 4.88 hsa-asg-5675hsa-asg-5675_st2 5.57 5.47 4.41 4.71 4.32 4.59 4.49 hsa-asg-8682hsa-asg-8682_st2 5.50 4.22 4.61 5.09 4.80 4.11 4.79 LNneg MicroRNA 7 8 9M SD % ttest log2Diff Fold hsa-miR-206 8.45 4.44 4.97 5.32 2.31 447.80E−03 −8.59 385.6 hsa-miR-205 3.38 5.30 7.74 4.71 1.85 33 2.48E−03−8.45 349.9 hsa-miR-375 1.00 4.61 6.37 4.27 1.82 0 4.07E−03 −7.63 198.6hsa-miR-381 3.48 1.28 3.22 3.08 0.91 0 1.28E−04 −6.63 98.9 hsa-miR-4994.80 4.55 3.84 4.87 0.85 22 7.86E−05 −6.63 98.7 hsa-miR-499 5.03 4.254.58 4.86 0.74 22 3.10E−05 −6.54 93.1 hsa-miR-133b 7.20 4.44 5.36 6.531.79 67 8.48E−03 −6.54 92.9 hsa-miR-203 5.17 6.05 9.34 6.77 1.38 782.22E−03 −6.45 87.6 hsa-miR-429 2.31 2.41 5.36 2.86 1.14 0 7.79E−04−6.30 78.7 hsa-miR-299-5p 5.03 2.41 3.94 4.74 1.40 44 2.99E−03 −6.2073.7 hsa-miR-411 3.38 2.23 2.07 3.83 1.27 11 1.85E−03 −6.13 69.9hsa-miR-133a 8.20 6.23 6.46 7.59 1.47 89 5.17E−03 −5.89 59.4hsa-miR-493-5p 4.36 1.85 4.58 4.66 1.58 33 9.25E−03 −5.66 50.5hsa-asg-2027 4.25 4.19 4.51 3.94 1.01 0 8.48E−04 −5.51 45.5 hsa-miR-200a4.05 4.75 7.76 5.54 1.15 67 1.89E−03 −5.50 45.3 hsa-miR-200b 6.50 7.6410.4 7.68 1.27 100 3.86E−03 −5.37 41.4 hsa-asg-14153 1.17 2.74 4.37 2.730.85 0 3.77E−04 −5.26 38.4 hsa-miR-539 3.48 1.66 2.07 2.19 0.63 04.96E−05 −5.23 37.4 hsa-miR-539 1.96 2.58 1.36 2.52 0.63 0 5.96E−05−5.10 34.4 hsa-miR-378 6.14 3.56 0.64 4.25 1.87 22 3.54E−02 −4.99 31.8hsa-miR-368 5.62 3.97 5.07 6.25 1.40 67 1.14E−02 −4.83 28.5 hsa-asg-56171.42 1.46 1.60 2.78 1.03 0 2.20E−03 −4.79 27.7 hsa-miR-495 5.14 2.414.87 5.23 1.36 56 1.16E−02 −4.67 25.5 hsa-miR-200c 7.96 8.87 11.5 9.051.21 100 7.35E−03 −4.56 23.6 hsa-asg-2345 3.15 2.58 1.36 2.35 0.76 04.69E−04 −4.55 23.5 hsa-asg-3376 2.47 4.19 3.36 3.82 0.77 0 5.36E−04−4.53 23.1 hsa-miR-376a 2.47 3.80 3.73 4.34 1.02 0 3.10E−03 −4.49 22.4hsa-miR-376a 2.90 4.04 1.83 3.57 0.86 0 1.48E−03 −4.31 19.8 hsa-miR-376a3.83 4.12 4.64 4.83 0.75 11 7.04E−04 −4.23 18.7 hsa-asg-7637 3.27 3.615.24 3.78 0.77 0 5.67E−04 −4.46 22.1 hsa-miR-576 1.96 2.05 3.06 2.310.52 0 4.13E−05 −4.44 21.6 hsa-miR-487b 4.88 3.71 4.37 5.58 1.11 675.34E−03 −4.43 21.6 hsa-cand14 2.31 2.41 3.50 2.20 0.65 0 2.05E−04 −4.4321.5 hsa-asg-1794 2.14 1.46 2.89 1.97 0.57 0 8.28E−05 −4.38 20.8hsa-asg-5021 4.57 3.03 2.07 4.63 1.58 22 3.14E−02 −4.34 20.2hsa-asg-9198 1.60 1.09 1.83 1.60 0.36 0 3.95E−06 −4.25 19.1 hsa-cand2063.57 1.28 2.71 2.65 0.86 0 1.61E−03 −4.22 18.6 hsa-asg-2399 1.09 2.232.51 2.52 0.67 0 3.45E−04 −4.19 18.2 hsa-asg-7472 3.57 0.92 0.93 1.970.85 0 1.60E−03 −4.17 18.0 hsa-miR-141 3.83 3.80 4.51 4.16 0.69 04.63E−04 −4.15 17.8 hsa-asg-11715 0.63 1.46 3.36 1.90 0.74 0 7.38E−04−4.13 17.5 hsa-miR-605 2.90 3.03 4.44 2.52 1.03 0 5.33E−03 −4.12 17.4hsa-asg-8428 0.04 2.14 1.60 1.79 0.81 0 1.32E−03 −4.12 17.3hsa-asg-14065 2.90 1.37 2.40 2.75 1.39 0 2.31E−02 −4.09 17.1hsa-asg-13254 4.71 2.41 3.36 4.86 1.37 56 2.34E−02 −4.04 16.5hsa-asg-791 0.50 1.46 2.51 1.98 0.72 0 7.17E−04 −4.02 16.2 hsa-miR-3796.33 6.13 6.20 6.58 0.37 100 6.68E−06 −4.01 16.1 hsa-asg-2484 3.38 2.742.51 2.57 0.74 0 1.13E−03 −3.88 14.7 hsa-miR-127 6.57 5.47 5.42 6.511.03 56 7.77E−03 −3.84 14.3 hsa-asg-13404 2.14 1.09 2.29 2.82 1.29 02.58E−02 −3.72 13.1 hsa-asg-1826 2.90 3.10 1.83 2.72 0.59 0 3.59E−04−3.67 12.7 hsa-miR-517c 1.42 2.23 2.07 2.70 0.70 0 1.16E−03 −3.63 12.4hsa-asg-1296 3.15 3.88 4.13 3.24 0.85 0 3.80E−03 −3.62 12.3 hsa-miR-34c3.48 3.71 6.83 3.57 1.38 11 3.77E−02 −3.61 12.2 hsa-miR-376b 2.77 2.500.22 2.28 1.17 0 1.95E−02 −3.58 11.9 hsa-asg-11777 2.90 2.23 3.06 2.480.60 0 4.60E−04 −3.58 11.9 hsa-miR-369-5p 3.38 2.74 3.50 3.36 0.56 03.09E−04 −3.57 11.9 hsa-miR-369-5p 2.90 2.58 3.50 3.42 0.81 0 6.19E−03−3.13 8.7 hsa-asg-10250 3.48 2.05 1.14 2.04 0.74 0 1.87E−03 −3.57 11.9hsa-asg-12139 1.60 2.05 3.50 2.32 0.59 0 4.35E−04 −3.56 11.8 hsa-miR-1543.15 1.28 3.94 3.47 1.10 0 1.60E−02 −3.54 11.6 hsa-asg-10131 2.62 3.175.07 3.22 0.79 0 2.76E−03 −3.54 11.6 hsa-asg-128 4.96 4.32 4.13 4.580.35 0 1.35E−05 −3.50 11.3 hsa-asg-13705 0.37 3.17 3.22 2.27 1.11 01.75E−02 −3.49 11.2 hsa-asg-10393 1.78 3.51 2.51 2.66 0.74 0 2.21E−03−3.47 11.0 hsa-asg-11088 1.60 3.35 4.48 2.29 1.30 0 3.57E−02 −3.45 10.9hsa-asg-1765 3.27 2.23 2.07 2.56 0.55 0 3.78E−04 −3.41 10.6 hsa-asg-9242.14 2.74 3.50 3.38 0.89 0 7.12E−03 −3.38 10.4 hsa-miR-187 3.03 3.353.14 3.33 0.61 0 7.92E−04 −3.38 10.4 hsa-asg-12142 2.77 1.09 2.29 2.290.70 0 1.77E−03 −3.37 10.3 hsa-miR-517a 4.05 3.88 2.51 4.25 1.23 223.51E−02 −3.29 9.7 hsa-asg-6178 0.78 2.23 2.29 2.29 0.93 0 1.05E−02−3.27 9.6 hsa-miR-489 5.71 5.40 5.20 6.18 0.84 56 6.33E−03 −3.27 9.6hsa-asg-13166 3.57 2.05 4.04 3.94 0.88 0 8.09E−03 −3.26 9.6 hsa-asg-51192.90 2.58 3.62 2.49 0.55 0 5.48E−04 −3.24 9.4 hsa-miR202 4.25 2.41 3.063.45 0.88 0 8.93E−03 −3.17 9.0 hsa-asg-13231 1.78 2.05 4.44 3.47 1.01 01.83E−02 −3.15 8.9 hsa-asg-3998 9.20 8.74 8.47 8.75 0.35 100 2.63E−05−3.15 8.9 hsa-asg-1619 2.31 3.03 3.22 3.30 0.78 0 5.18E−03 −3.15 8.9hsa-miR-432 3.48 4.25 2.71 4.80 1.13 22 3.05E−02 −3.12 8.7hsa-miR-493-3p 4.31 3.46 4.08 3.41 0.95 0 1.50E−02 −3.10 8.6 hsa-asg-542.90 1.85 2.71 2.16 0.66 0 2.34E−03 −3.07 8.4 hsa-miR-329 3.66 2.74 3.063.16 0.84 0 8.78E−03 −3.06 8.3 hsa-miR-329 2.77 3.29 2.71 3.41 0.74 01.52E−02 −2.39 5.2 hsa-miR-329 3.98 2.58 3.50 3.58 0.59 0 4.72E−02 −1.472.8 hsa-miR-410 3.15 1.85 2.51 2.51 0.68 0 2.82E−03 −3.05 8.3hsa-asg-378 1.09 2.74 3.06 2.71 0.96 0 1.66E−02 −3.05 8.3 hsa-asg-138783.03 2.41 1.83 3.21 0.90 0 1.37E−02 −3.00 8.0 hsa-asg-13070 2.31 1.851.36 2.54 0.79 0 7.55E−03 −2.97 7.8 hsa-miR-128a 9.00 8.75 8.66 9.330.70 100 3.97E−03 −2.94 7.7 hsa-miR-422a 9.67 9.22 9.12 9.20 0.33 1003.00E−05 −2.94 7.7 hsa-asg-3822 1.60 2.89 4.76 2.72 0.99 0 2.27E−02−2.93 7.6 hsa-miR-189 4.92 3.51 4.92 5.33 1.09 44 3.49E−02 −2.91 7.5hsa-miR-200a* 2.14 3.17 2.89 2.72 0.64 0 2.67E−03 −2.91 7.5 hsa-asg-12033.91 3.71 1.83 3.70 0.90 0 1.60E−02 −2.89 7.4 hsa-asg-4439 2.77 3.714.04 3.31 0.84 0 1.15E−02 −2.89 7.4 hsa-miR-299-3p 1.60 3.17 3.50 2.750.78 0 8.25E−03 −2.88 7.3 hsa-miR-31 7.71 8.66 10.0 9.21 0.92 1001.89E−02 −2.83 7.1 hsa-miR-338 4.76 3.40 4.87 4.30 1.04 11 3.33E−02−2.82 7.1 hsa-asg-9365 2.77 2.74 2.07 2.47 0.43 0 2.90E−04 −2.78 6.9hsa-miR-128b 9.53 9.18 9.23 9.75 0.70 100 5.58E−03 −2.77 6.8hsa-asg-9723 2.31 3.66 2.07 2.46 0.70 0 5.60E−03 −2.76 6.8 hsa-asg-28442.77 2.58 2.51 2.85 0.71 0 5.98E−03 −2.76 6.8 hsa-miR-582 4.05 4.32 4.644.49 1.12 22 4.90E−02 −2.75 6.7 hsa-miR-95 9.08 7.80 8.19 9.00 0.80 1001.22E−02 −2.71 6.5 hsa-miR-154* 4.25 3.17 4.37 4.31 0.79 11 1.16E−02−2.71 6.5 hsa-asg-13997 1.25 3.17 2.29 2.51 0.65 0 4.31E−03 −2.70 6.5hsa-asg-11160 2.47 3.51 5.11 3.21 0.79 0 1.21E−02 −2.68 6.4hsa-asg-10947 3.57 2.58 2.51 3.32 0.98 0 3.17E−02 −2.68 6.4 hsa-asg-71593.98 3.40 2.29 3.35 0.62 0 3.64E−03 −2.67 6.3 hsa-miR-96 3.03 1.85 3.063.24 0.87 11 1.94E−02 −2.66 6.3 hsa-asg-1587 2.77 2.41 2.51 2.42 0.51 01.11E−03 −2.66 6.3 hsa-asg-8174 2.77 2.74 4.13 3.21 0.53 0 1.47E−03−2.65 6.3 hsa-miR-422b 10.94 10.7 10.5 10.7 0.28 100 2.07E−05 −2.64 6.2hsa-asg-1270 3.03 3.29 3.22 3.63 0.90 0 2.53E−02 −2.60 6.1 hsa-asg-103603.15 2.41 2.71 3.29 0.63 0 5.38E−03 −2.53 5.8 hsa-cand207 2.62 2.74 3.223.19 0.60 0 4.21E−03 −2.49 5.6 hsa-asg-8616 3.83 2.05 3.06 3.41 1.02 04.92E−02 −2.48 5.6 hsa-miR-382 6.27 5.91 5.79 6.70 0.61 78 5.29E−03−2.45 5.5 hsa-cand330 2.62 3.97 3.94 4.19 0.80 11 2.19E−02 −2.38 5.2hsa-asg-4283 3.48 2.23 2.51 3.19 0.65 0 8.46E−03 −2.38 5.2 hsa-miR-9*4.92 4.71 5.20 5.55 0.53 44 3.24E−03 −2.33 5.0 hsa-asg-13471 4.36 4.043.50 4.61 0.61 11 6.89E−03 −2.32 5.0 hsa-miR-183 7.50 6.30 6.63 6.970.72 89 1.56E−02 −2.31 5.0 hsa-asg-4840 4.62 4.55 4.37 4.80 0.61 117.43E−03 −2.29 4.9 hsa-asg-11361 3.83 2.58 4.29 3.39 0.52 0 3.12E−03−2.27 4.8 hsa-asg-1596 4.12 4.19 4.37 4.20 0.49 0 2.43E−03 −2.25 4.7hsa-asg-13888 2.62 1.85 2.71 2.24 0.60 0 7.71E−03 −2.23 4.7hsa-asg-11883 6.11 4.55 6.42 6.06 0.64 78 1.09E−02 −2.23 4.7 hsa-miR-93.48 3.29 4.13 3.29 0.55 0 5.32E−03 −2.19 4.6 hsa-asg-12515 3.66 4.123.73 3.98 0.45 0 1.74E−03 −2.18 4.5 hsa-asg-10236 3.15 3.71 3.36 3.800.68 0 1.59E−02 −2.18 4.5 hsa-asg-13135 5.14 4.44 5.57 5.11 0.44 221.52E−03 −2.17 4.5 hsa-asg-3251 2.90 3.03 3.22 3.52 0.55 0 6.41E−03−2.11 4.3 hsa-asg-11923 2.31 2.41 3.62 3.52 0.73 0 2.66E−02 −2.10 4.3hsa-asg-4140 4.12 3.71 2.71 3.58 0.49 0 3.60E−03 −2.08 4.2 hsa-asg-24191.60 2.41 3.22 2.49 0.62 0 1.27E−02 −2.08 4.2 hsa-asg-67 3.66 3.51 3.623.51 0.61 0 1.29E−02 −2.05 4.1 hsa-miR-494 5.71 5.84 6.58 6.68 0.64 1001.77E−02 −2.01 4.0 hsa-miR-409-5p 5.90 5.43 5.24 5.63 0.58 44 1.12E−02−2.01 4.0 hsa-cand443 4.84 3.80 4.29 4.59 0.68 33 2.51E−02 −1.96 3.9hsa-asg-9514 4.18 2.58 3.36 3.43 0.79 0 4.98E−02 −1.91 3.8 hsa-miR-135b3.57 1.66 3.22 3.18 0.75 0 4.41E−02 −1.89 3.7 hsa-asg-10478 3.66 2.742.71 3.39 0.69 0 3.24E−02 −1.88 3.7 hsa-asg-10679 3.15 3.29 3.06 3.440.64 0 2.49E−02 −1.85 3.6 hsa-asg-10881 4.18 5.01 5.20 5.19 0.55 221.36E−02 −1.82 3.5 hsa-miR-134 4.71 4.29 5.00 5.16 0.68 0 4.74E−02 −1.693.2 hsa-miR-486 9.77 9.32 7.96 9.01 0.65 100 4.31E−02 −1.65 3.1hsa-asg-11397 3.27 3.51 3.36 3.66 0.31 0 1.13E−03 −1.61 3.1 hsa-asg-37423.57 4.19 3.62 4.03 0.55 0 2.63E−02 −1.57 3.0 hsa-asg-6803 3.15 3.514.13 4.00 0.62 11 4.49E−02 −1.56 2.9 hsa-asg-9009 4.92 3.66 3.99 4.590.61 0 4.56E−02 −1.52 2.9 hsa-asg-14233 9.03 8.85 8.87 9.23 0.41 1009.66E−03 −1.48 2.8 hsa-asg-807 4.57 4.97 5.54 4.86 0.52 11 3.11E−02−1.44 2.7 hsa-asg-13279 6.86 6.98 6.66 7.22 0.52 100 3.47E−02 −1.39 2.6hsa-asg-5149 3.66 4.78 4.90 4.86 0.51 0 3.67E−02 −1.35 2.5 hsa-asg-111815.33 5.03 5.18 5.55 0.43 11 1.83E−02 −1.34 2.5 hsa-asg-7471 5.24 4.976.07 5.65 0.52 56 4.37E−02 −1.31 2.5 hsa-asg-8476 6.75 5.40 5.61 6.040.47 11 2.91E−02 −1.31 2.5 hsa-miR-628 7.23 7.64 7.43 7.60 0.37 1001.07E−02 −1.30 2.5 hsa-miR-210 6.09 6.46 7.00 6.59 0.50 89 3.82E−02−1.30 2.5 hsa-asg-4438 4.18 4.80 5.32 4.61 0.39 0 1.50E−02 −1.26 2.4hsa-asg-12179 4.80 5.01 5.32 5.29 0.43 11 3.50E−02 −1.16 2.2hsa-asg-5675 4.42 4.22 4.17 4.53 0.39 0 3.48E−02 −1.04 2.1 hsa-asg-86823.75 4.61 4.21 4.46 0.42 0 4.84E−02 −1.03 2.0 SD, standard deviation; %,percentage of samples expressing miRNA above background levels;Log2Diff, difference in VSN-transformed expression bewteen TNG and LNnegsamples.

Example 34 miRNAs Differentally Expressed Between Normal Brain Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous brain cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal brain tissuethan in cancer-negative lymph nodes.

Total RNA, purified from one normal human brain sample (FirstChoice®Human Brain Total RNA, cat. no. AM7962; Ambion, Austin, Tex., USA) andfrom nine normal human lymph node samples (FirstChoice® Human Lymph NodeTotal RNA, cat. no. AM7894; Ambion, Austin, Tex., USA) was purchasedfrom Ambion. miRNA purification and labeling and microarray expressionanalyses were performed as described above in Example 2.

The inventors identified 180 miRNAs (81 verified, 99 predicted) withsignificant differential expression between the normal brain and normallymph node samples (log 2Diff brain vs LN neg≧1 and p-value<0.05) (Table33). Among the verified miRNAs, hsa-miR-124a was expressed at a levelmore than 1000-fold higher in the brain sample; 5 miRNAs (hsa-miR-433,-383, -129, -487b, and -323) were expressed at levels between 100- and210-fold higher in brain; 12 miRNAs were expressed at levels between 50-and 100-fold higher in brain; 21 miRNAs were expressed at levels between20- and 50-fold higher in brain; 15 miRNAs were expressed at levelsbetween 10- and 20-fold higher in brain; and 15 miRNAs were expressed atlevels between 5- and 10-fold higher in the brain sample than in normallymph nodes. Among the predicted miRNAs, hsa-asg-13159_st1 was expressedat a level over 1000-fold higher in the brain sample; 3 miRNAs(hsa-miR-13404 st2, -3373_st2 and hsa-miR202_st2) were expressed atlevels between 100- and 200-fold higher in brain; 4 miRNAs wereexpressed at levels between 50- and 100-fold higher in brain; 10 miRNAswere expressed at levels between 20- and 50-fold higher in brain; 20miRNAs were expressed at levels between 10- and 20-fold higher in brain;and 27 miRNAs were expressed at levels between 5- and 10-fold higher inthe brain sample than in normal lymph nodes.

TABLE 33 MicroRNAs Differentially Expressed Between Normal Brain Samples(BRN) and Cancer-Negative Lymph Node Samples (LNneg) from Patients.Probe LNneg MicroRNA ID BRN 1 2 3 4 5 6 7 hsa-miR-124a hsa-cand79_st113.89 3.53 3.78 3.78 4.44 3.64 2.14 2.31 hsa-miR-124a hsa-miR-124a_st113.76 4.29 4.18 3.78 4.80 3.81 3.32 2.14 hsa-asg-13159 hsa-asg-13159_st113.49 3.18 3.11 4.01 3.05 4.11 2.65 2.62 hsa-miR-433 hsa-miR-433_st210.49 2.41 2.67 4.54 2.90 4.17 2.32 1.60 hsa-asg-13404 hsa-asg-13404_st210.25 4.15 2.67 5.28 2.90 3.24 1.57 2.14 hsa-miR-383 hsa-miR-383_st19.70 3.31 2.83 2.40 2.57 3.18 1.30 2.62 hsa-asg-3373 hsa-asg-3373_st29.88 3.53 1.94 2.63 3.75 4.04 3.63 1.78 hsa-miR-129 hsa-miR-129_st111.47 4.84 4.76 5.24 4.80 4.17 3.89 4.47 hsa-miR-487b hsa-miR-487b_st212.44 6.05 5.79 6.97 6.90 6.22 5.34 4.88 hsa-miR202 hsa-miR202_st2 10.293.83 2.67 4.93 3.94 3.59 2.40 4.25 hsa-miR-323 hsa-miR-323_st1 10.724.15 3.42 4.01 5.76 5.09 2.94 3.62 hsa-miR-329 hsa-asg-13589_st2 9.721.63 3.11 3.78 3.71 4.38 2.32 3.66 hsa-miR-329 hsa-miR-329_st1 9.94 3.732.97 4.61 3.25 4.54 2.80 2.77 hsa-miR-329 hsa-asg-13204_st2 9.81 4.072.83 4.21 3.38 4.20 3.43 3.98 hsa-miR-485-3p hsa-miR23_st2 9.77 4.292.50 4.11 3.25 2.41 3.20 3.57 hsa-miR-485-3p hsa-miR-485-3p_st2 9.683.64 2.97 4.30 4.41 4.24 3.08 3.66 hsa-miR-539 hsa-miR-539_st1 9.01 2.033.11 2.63 3.38 2.65 2.94 1.96 hsa-miR-539 hsa-miR177_st1 8.42 2.58 2.501.37 2.39 1.80 1.86 3.48 hsa-miR-9 hsa-miR-9_st2 9.71 3.42 3.48 2.843.25 3.59 2.14 3.48 hsa-miR-9 hsa-cand90_st2 8.39 3.53 3.11 2.63 2.982.79 2.14 2.62 hsa-cand26 hsa-cand26_st2 11.37 6.11 5.56 6.04 4.06 3.644.70 5.27 hsa-miR-410 hsa-miR-410_st1 8.90 2.41 2.32 1.37 3.71 2.49 2.803.15 hsa-miR-149 hsa-miR-149_st2 9.39 4.47 3.82 1.76 4.14 2.72 1.48 2.47hsa-asg-13135 hsa-asg-13135_st1 11.46 5.61 5.61 5.24 4.89 4.76 4.70 5.14hsa-asg-4574 hsa-asg-4574_st2 9.73 3.42 2.83 3.66 2.82 4.90 3.20 3.27hsa-miR-495 hsa-miR-495_st1 11.49 6.01 4.76 6.97 6.25 6.29 4.38 5.14hsa-miR-485-5p hsa-miR-485-5p_st2 9.22 3.42 3.24 3.38 3.66 3.24 1.953.48 hsa-miR-432 hsa-miR-432_st2 10.87 4.53 5.38 5.93 5.52 5.74 5.623.48 hsa-miR-487a hsa-miR-487a_st2 8.51 2.22 2.83 2.63 3.38 2.16 3.200.93 hsa-miR-487a hsa-asg-6915_st1 8.81 4.07 3.58 3.90 3.38 3.85 3.723.66 hsa-asg-10881 hsa-asg-10881_st1 11.02 5.71 4.98 6.06 5.43 4.76 5.404.18 hsa-miR-338 hsa-miR-338_st1 10.10 5.09 4.03 5.57 4.85 4.00 2.144.76 hsa-miR-137 hsa-miR-137_st1 8.38 2.58 2.32 3.38 1.90 2.33 3.32 2.14hsa-miR-346 hsa-miR-346_st1 8.85 2.75 2.03 2.52 3.75 4.44 2.65 3.48hsa-asg-7295 hsa-asg-7295_st2 7.99 2.90 2.13 3.38 1.59 2.07 2.49 2.77hsa-miR-411 hsa-miR-411_st1 9.37 4.84 3.68 4.54 5.22 5.52 2.94 3.38hsa-miR-592 hsa-miR-592_st1 8.40 4.29 2.50 2.84 3.12 1.80 1.76 3.03hsa-miR-592 hsa-asg-4400_st1 8.24 3.05 3.24 3.66 1.69 1.62 2.65 3.03hsa-miR-330 hsa-miR-330_st2 10.62 6.07 5.65 5.82 4.25 5.22 5.15 5.33hsa-miR-330 hsa-asg-14199_st1 10.49 5.88 5.23 6.15 5.26 5.30 4.85 5.10hsa-asg-13166 hsa-asg-13166_st2 9.29 4.47 3.36 4.87 4.94 4.07 4.12 3.57hsa-asg-5617 hsa-asg-5617_st1 8.05 3.73 3.24 3.90 3.60 3.40 2.65 1.42hsa-asg-4201 hsa-asg-4201_st1 7.22 1.43 1.94 1.37 1.90 2.33 2.49 2.47hsa-asg-7472 hsa-asg-7472_st2 7.14 1.63 2.13 1.89 2.00 2.86 1.76 3.57hsa-miR-299-5p hsa-miR-299-5p_st2 9.91 5.71 4.44 5.81 6.37 5.96 2.945.03 hsa-miR-328 hsa-miR-328_st1 10.13 7.38 1.15 5.90 7.34 4.54 4.125.78 hsa-asg-10202 hsa-asg-10202_st2 8.26 4.69 2.03 3.30 5.24 3.06 1.952.47 hsa-asg-3985 hsa-asg-3985_st1 7.23 2.22 2.50 1.37 1.69 2.24 2.490.78 hsa-miR-127 hsa-miR-127_st1 11.61 6.80 5.52 7.96 7.53 7.66 5.666.57 hsa-miR-380-3p hsa-miR-380-3p_st2 6.60 2.22 1.15 1.12 0.57 2.162.32 1.96 hsa-miR-380-3p hsa-asg-13118_st1 6.52 2.03 3.11 3.04 1.28 1.622.32 1.25 hsa-asg-14178 hsa-asg-14178_st2 6.89 1.05 1.15 2.63 1.69 3.640.87 0.26 hsa-miR-9* hsa-miR-9-_st2 10.58 6.19 6.05 5.57 6.10 5.37 5.814.92 hsa-asg-13284 hsa-asg-13284_st1 8.82 4.88 2.03 3.59 5.83 4.82 2.804.71 hsa-miR-379 hsa-miR-379_st1 11.51 6.51 6.37 7.12 6.96 7.01 6.546.33 hsa-miR-138 hsa-miR-138_st1 10.50 6.36 5.68 6.82 6.41 5.91 4.574.92 hsa-cand206 hsa-cand206_st1 7.51 2.22 1.94 3.78 3.60 2.65 2.14 3.57hsa-miR-584 hsa-miR-584_st2 10.60 5.74 6.05 6.24 6.23 5.72 5.72 5.24hsa-miR-132 hsa-miR-132_st1 13.06 9.71 8.51 8.34 8.34 8.56 8.02 8.00hsa-miR-382 hsa-miR-382_st1 11.32 7.16 6.80 7.35 7.13 7.40 6.51 6.27hsa-miR-154* hsa-miR-154-_st2 8.92 5.81 3.48 4.21 4.98 4.59 3.89 4.25hsa-miR-598 hsa-miR-598_st2 10.20 6.70 5.93 5.90 6.87 5.94 5.53 5.57hsa-miR-212 hsa-miR-212_st2 9.94 6.26 5.68 6.18 5.71 5.14 5.05 4.57hsa-miR-153 hsa-miR-153_st2 6.56 2.41 1.74 1.89 1.28 2.93 1.76 2.47hsa-miR-139 hsa-miR-139_st2 13.32 10.99 9.36 9.56 9.74 8.42 8.94 9.34hsa-miR-381 hsa-miR-381_st2 7.43 2.75 2.32 4.21 3.38 4.17 2.94 3.48hsa-miR-409-3p hsa-miR-409-3p_st1 8.07 2.22 3.42 5.14 4.98 4.73 2.943.95 hsa-asg-9757 hsa-asg-9757_st1 6.40 2.41 1.15 2.15 1.79 1.80 2.232.90 hsa-asg-2364 hsa-asg-2364_st1 5.74 1.05 0.97 0.66 2.00 1.35 1.392.77 hsa-asg-5021 hsa-asg-5021_st1 8.84 5.17 4.38 7.00 6.05 5.88 3.484.57 hsa-asg-4471 hsa-asg-4471_st1 6.81 1.63 2.97 2.84 2.98 2.79 2.492.62 hsa-miR-204 hsa-miR-204_st2 10.36 7.17 5.77 5.98 8.20 6.73 5.405.17 hsa-miR-128a hsa-miR-128a_st2 13.48 10.77 9.31 9.72 9.95 8.83 8.949.00 hsa-miR-105 hsa-miR-105_st1 6.15 1.63 1.34 2.63 0.82 2.72 1.39 2.47hsa-asg-13254 hsa-asg-13254_st1 8.98 5.58 5.32 6.66 5.89 5.87 3.97 4.71hsa-asg-10304 hsa-asg-10304_st1 6.36 1.43 1.54 1.89 2.48 2.33 1.95 2.14hsa-cand660 hsa-cand660_st1 6.18 2.22 1.74 1.37 1.48 2.65 1.57 2.77hsa-miR-369-5p hsa-asg-1521_st1 7.42 3.73 3.36 4.01 2.20 3.68 3.68 3.38hsa-miR-369-5p hsa-miR-369-5p_st2 7.18 3.53 2.67 4.46 4.35 4.33 2.492.90 hsa-asg-12824 hsa-asg-12824_st1 6.20 2.03 1.74 3.04 1.90 2.49 1.572.62 hsa-asg-924 hsa-asg-924_st1 7.41 4.64 4.25 3.38 4.44 2.65 2.73 2.14hsa-asg-9303 hsa-asg-9303_st2 6.19 1.43 2.67 1.12 3.05 2.93 1.86 2.62hsa-miR-128b hsa-miR-128b_st1 13.63 11.26 9.78 10.01 10.35 9.17 9.279.53 hsa-miR-184 hsa-miR-184_st1 7.97 5.50 4.50 4.11 4.28 3.89 5.11 1.60hsa-miR-370 hsa-miR-370_st1 8.69 4.64 5.23 4.71 5.05 4.69 5.34 4.31hsa-asg-7465 hsa-asg-7465_st2 8.69 4.64 4.31 4.01 6.17 5.05 4.15 5.14hsa-asg-9365 hsa-asg-9365_st2 6.26 3.31 2.13 1.89 2.48 2.49 2.32 2.77hsa-asg-6667 hsa-asg-6667_st1 5.54 2.41 2.13 2.40 0.57 1.89 1.76 1.42hsa-miR-491 hsa-miR-491_st1 9.24 5.24 6.12 5.04 5.61 5.51 5.70 5.12hsa-miR-134 hsa-miR-134_st2 8.91 5.09 5.73 6.00 5.84 5.67 4.15 4.71hsa-miR-154 hsa-miR-154_st2 7.18 3.73 2.50 4.30 4.35 4.88 3.08 3.15hsa-asg-9514 hsa-asg-9514_st1 7.14 2.58 4.50 3.21 2.48 4.33 3.63 4.18hsa-miR-187 hsa-miR-187_st1 7.00 4.74 2.75 2.74 3.75 3.18 3.32 3.03hsa-miR-409-5p hsa-miR-409-5p_st1 9.29 5.13 4.98 6.68 6.08 6.08 5.115.90 hsa-asg-5455 hsa-asg-5455_st2 5.83 2.41 2.97 3.04 0.90 2.16 1.951.78 hsa-miR-376a hsa-miR-376a_st2 7.97 5.13 4.03 5.49 5.66 4.82 3.972.47 hsa-miR-376a hsa-asg-14234_st1 7.18 3.31 4.25 3.38 3.98 4.82 3.632.90 hsa-miR-376a hsa-asg-10049_st1 8.33 5.01 4.85 6.09 5.55 5.31 4.053.83 hsa-asg-14020 hsa-asg-14020_st2 5.51 1.63 2.97 1.12 2.00 2.16 1.391.09 hsa-asg-2637 hsa-asg-2637_st1 7.67 4.35 4.14 4.96 4.53 4.24 3.383.38 hsa-asg-9971 hsa-asg-9971_st2 7.11 4.29 3.48 2.84 3.71 3.59 3.324.18 hsa-miR-7 hsa-miR-7_st1 12.00 9.45 8.18 9.71 8.61 8.16 7.65 8.64hsa-miR-577 hsa-miR-577_st1 6.15 4.59 2.83 3.21 2.98 2.79 1.39 1.96hsa-asg-12250 hsa-asg-12250_st2 5.40 2.75 2.13 1.89 1.48 1.98 1.76 2.31hsa-asg-10106 hsa-asg-10106_st1 5.51 3.31 2.32 1.63 1.28 2.41 1.76 1.96hsa-asg-10439 hsa-asg-10439_st1 5.46 2.22 1.94 2.84 1.69 2.16 1.57 1.96hsa-asg-14176 hsa-asg-14176_st1 8.76 6.05 5.67 5.28 6.77 6.23 5.37 5.44hsa-asg-10916 hsa-asg-10916_st2 5.64 2.58 2.32 2.63 2.10 2.79 1.76 2.90hsa-asg-11880 hsa-asg-11880_st1 5.69 3.73 2.50 2.15 2.30 2.79 2.32 2.90hsa-miR-181d hsa-asg-13508_st1 9.23 6.60 5.77 6.86 6.60 5.60 5.62 5.67hsa-asg-8067 hsa-asg-8067_st2 9.14 6.66 6.35 5.82 6.62 6.65 5.69 5.30hsa-miR-299-3p hsa-asg-10378_st1 5.90 3.64 3.36 3.21 1.90 2.41 1.95 1.60hsa-miR-299-3p hsa-miR-299-3p_st2 5.91 2.41 3.36 3.38 2.90 3.40 2.733.27 hsa-asg-12177 hsa-asg-12177_st2 6.79 4.74 3.36 3.52 4.62 3.06 3.933.03 hsa-asg-13496 hsa-asg-13496_st1 6.15 3.18 2.50 2.40 3.44 3.12 3.323.27 hsa-asg-14159 hsa-asg-14159_st2 6.37 3.18 2.83 3.78 4.17 2.57 2.653.03 hsa-miR-499 hsa-miR12_st1 7.95 5.44 4.76 5.37 6.66 4.07 4.38 4.80hsa-miR-499 hsa-miR-499_st1 7.66 5.31 4.61 4.75 6.57 4.49 4.12 5.03hsa-cand165 hsa-cand165_st1 6.70 4.22 4.18 3.21 4.14 3.55 4.19 1.60hsa-miR-628 hsa-miR-628_st2 10.66 8.29 7.83 7.21 8.00 7.32 7.47 7.23hsa-asg-2261 hsa-asg-2261_st1 6.05 3.53 3.11 2.84 2.98 2.86 3.63 1.60hsa-miR-494 hsa-miR-494_st1 9.66 6.39 7.25 7.23 6.95 6.60 7.61 5.71hsa-miR-494 hsa-miR61_st2 8.90 6.81 6.50 6.86 6.42 6.35 7.05 5.38hsa-miR-340 hsa-miR-340_st1 7.65 5.81 4.94 5.24 5.70 4.86 4.41 4.47hsa-miR-504 hsa-miR-504_st1 7.39 4.29 4.74 2.52 5.95 5.81 4.22 3.83hsa-miR-181d hsa-miR-181d_st1 11.20 9.12 7.78 8.91 8.92 7.57 7.91 8.26hsa-miR-181d hsa-miR74_st1 10.79 8.96 8.17 9.00 8.79 7.99 8.20 8.08hsa-asg-5083 hsa-asg-5083_st1 5.31 2.58 2.32 2.40 2.48 2.33 2.14 2.90hsa-miR-189 hsa-miR-189_st1 8.21 6.07 5.74 4.93 7.23 6.15 4.49 4.92hsa-asg-11040 hsa-asg-11040_st2 5.57 2.75 2.32 3.38 2.30 2.99 2.94 3.27hsa-asg-1388 hsa-asg-1388_st1 5.59 2.03 3.58 2.15 2.10 3.55 2.80 2.62hsa-asg-13258 hsa-asg-13258_st2 10.65 9.04 8.90 7.04 8.65 7.48 8.01 7.17hsa-asg-13646 hsa-asg-13646_st1 10.04 8.23 8.08 6.97 7.98 6.52 7.51 6.66hsa-asg-13231 hsa-asg-13231_st1 6.22 3.91 4.11 4.21 4.44 2.93 3.38 1.78hsa-asg-11949 hsa-asg-11949_st1 6.89 4.88 4.55 4.01 3.94 3.68 3.93 3.15hsa-asg-14175 hsa-asg-14175_st1 8.88 6.55 6.31 6.43 7.06 6.07 6.08 5.57hsa-asg-12515 hsa-asg-12515_st2 6.56 4.00 4.25 3.04 4.10 4.57 4.32 3.66hsa-miR-331 hsa-asg-14205_st1 9.01 7.52 5.80 7.38 7.77 6.83 4.72 6.62hsa-miR-331 hsa-miR-331_st2 10.34 9.03 7.52 8.36 8.78 7.66 7.04 7.68hsa-asg-4283 hsa-asg-4283_st2 5.72 3.91 3.48 2.63 3.80 3.89 2.80 3.48hsa-miR-181b hsa-miR-181b_st2 13.01 11.22 10.43 10.98 10.87 9.81 10.2610.24 hsa-cand208 hsa-cand208_st1 5.51 2.58 3.48 3.04 3.12 3.29 3.531.78 hsa-miR-218 hsa-miR-218_st2 11.23 9.61 9.22 8.83 9.68 9.13 8.597.75 hsa-asg-13878 hsa-asg-13878_st2 5.64 2.90 3.48 2.84 4.82 4.24 3.323.03 hsa-miR-551b hsa-miR167_st2 6.77 5.24 4.38 3.78 5.84 3.35 3.97 4.80hsa-miR-551b hsa-miR-551b_st1 6.30 4.79 4.81 4.87 4.75 3.97 2.65 4.12hsa-asg-4553 hsa-asg-4553_st1 5.92 2.41 2.97 3.66 4.14 4.07 3.81 3.03hsa-miR-181a* hsa-miR-213_st1 8.20 6.29 6.17 5.68 5.71 5.42 5.37 5.30hsa-asg-3376 hsa-asg-3376_st1 6.21 3.83 2.97 4.87 4.65 3.97 4.12 2.47hsa-asg-2335 hsa-asg-2335_st1 5.49 4.00 2.97 4.11 2.98 2.93 2.32 3.57hsa-asg-4439 hsa-asg-4439_st2 5.67 3.83 4.31 3.21 2.66 1.62 3.63 2.77hsa-miR-421 hsa-asg-5033_st2 10.55 8.21 8.07 8.49 8.61 7.77 7.98 7.76hsa-miR-421 hsa-miR-421-3p_st1 11.25 9.49 9.15 8.99 9.42 8.68 8.78 8.36hsa-miR-31 hsa-miR-31_st2 11.57 10.40 9.74 8.95 10.15 8.68 8.54 7.71hsa-cand244 hsa-cand244_st1 7.02 5.44 4.58 4.84 4.38 4.86 5.28 4.36hsa-asg-12164 hsa-asg-12164_st2 6.21 4.64 4.31 5.45 4.06 2.99 2.65 4.05hsa-miR-324-5p hsa-miR-324-5p_st1 10.20 7.91 8.08 8.34 8.26 7.77 7.377.80 hsa-asg-8476 hsa-asg-8476_st1 8.27 5.58 6.36 6.43 6.32 5.75 6.176.75 hsa-miR-654 hsa-asg-33_st1 6.74 3.18 6.20 5.09 4.51 4.41 5.15 4.57hsa-asg-8776 hsa-asg-8776_st2 5.64 3.83 2.83 4.38 3.94 2.57 3.89 2.90hsa-asg-8266 hsa-asg-8266_st2 6.36 4.15 3.78 4.61 5.22 4.41 4.70 4.47hsa-asg-879 hsa-asg-879_st1 5.49 3.91 2.97 3.66 4.21 2.86 3.63 2.90hsa-cand330 hsa-cand330_st2 6.18 4.74 4.98 3.52 4.70 5.12 4.12 2.62hsa-asg-12183 hsa-asg-12183_st1 5.91 4.69 4.03 4.11 3.94 4.14 4.41 1.96hsa-miR-181c hsa-miR-181c_st1 10.81 9.24 8.57 9.33 9.09 8.64 7.66 9.05hsa-asg-13279 hsa-asg-13279_st1 9.08 7.57 7.42 7.55 8.17 7.21 6.54 6.86hsa-asg-4294 hsa-asg-4294_st2 5.37 4.29 3.11 4.54 4.06 3.18 3.08 2.31hsa-cand443 hsa-cand443_st2 6.40 3.91 5.10 3.90 4.48 5.73 5.21 4.84hsa-asg-191 hsa-asg-191_st1 5.49 3.31 3.86 2.84 3.55 3.81 4.05 3.98hsa-asg-14138 hsa-asg-14138_st2 6.64 5.76 4.52 5.35 5.49 4.88 5.26 4.12hsa-miR-99b hsa-miR-99b_st1 12.28 10.99 10.46 10.53 11.45 11.1 10.0810.15 hsa-miR-99b hsa-asg-14196_st2 12.35 11.28 10.60 10.63 11.69 11.210.27 10.31 hsa-asg-4493 hsa-asg-4493_st1 5.40 4.22 3.36 4.46 2.98 3.594.05 2.77 hsa-asg-12179 hsa-asg-12179_st1 6.98 6.15 4.98 5.45 5.67 5.364.88 4.80 hsa-asg-1467 hsa-asg-1467_st2 6.69 6.03 5.28 3.66 5.24 4.925.43 5.10 hsa-asg-9646 hsa-asg-9646_st2 5.49 4.59 3.48 4.38 3.80 3.293.81 3.91 hsa-asg-13293 hsa-asg-13293_st1 5.43 3.91 3.24 4.21 4.10 4.472.94 3.83 hsa-miR-425-3p hsa-miR-425_st2 7.87 6.58 6.61 6.42 6.87 6.405.96 5.84 hsa-asg-3145 hsa-asg-3145_st1 7.04 5.66 5.14 5.28 6.40 6.225.53 4.96 hsa-asg-3405 hsa-asg-3405_st1 5.46 4.07 4.03 3.78 4.41 3.813.63 3.66 hsa-asg-6543 hsa-asg-6543_st1 5.46 4.22 3.58 4.11 4.25 4.303.89 3.48 hsa-asg-13471 hsa-asg-13471_st1 6.18 4.74 4.71 4.38 5.11 5.315.34 4.36 hsa-asg-11199 hsa-asg-11199_st2 13.06 12.25 11.85 11.24 12.2811.9 11.42 11.31 hsa-asg-6050 hsa-asg-6050_st2 5.40 3.73 4.25 3.52 3.554.57 4.25 3.48 hsa-miR-376a* hsa-miR-376a-_st2 5.92 4.79 4.11 5.33 5.114.49 4.38 3.75 hsa-miR-98 hsa-miR-98_st1 12.38 11.57 11.02 11.17 11.5711.0 10.83 10.89 hsa-asg-7507 hsa-asg-7507_st2 5.92 3.73 4.50 4.46 4.324.47 5.07 4.05 hsa-miR-185 hsa-miR-185_st2 12.00 11.04 10.95 11.29 10.6810.0 10.19 10.45 hsa-asg-2460 hsa-asg-2460_st1 5.74 4.47 4.18 4.93 4.753.59 4.12 4.47 hsa-asg-5134 hsa-asg-5134_st2 5.83 4.93 4.50 4.87 4.354.94 4.25 4.80 hsa-miR-181a hsa-miR-181a_st1 13.32 12.53 11.68 12.2812.46 11.9 11.25 12.00 hsa-asg-4438 hsa-asg-4438_st2 5.81 4.53 4.50 5.044.53 4.11 4.49 4.18 hsa-let-7e hsa-let-7e_st1 13.47 12.65 12.76 12.3512.64 12.2 12.64 12.01 hsa-asg-10626 hsa-asg-10626_st2 6.02 5.13 5.144.61 5.36 4.96 4.25 4.84 hsa-miR-324-3p hsa-miR-324-3p_st1 8.51 7.657.25 7.35 8.09 7.53 6.86 7.39 hsa-asg-7335 hsa-asg-7335_st2 6.25 5.134.25 5.41 5.07 5.16 5.28 5.20 hsa-asg-917 hsa-asg-917_st2 6.26 5.69 5.254.61 4.89 5.89 4.79 5.00 hsa-asg-12275 hsa-asg-12275_st1 7.39 5.90 5.726.76 6.49 6.43 6.32 6.19 hsa-asg-6462 hsa-asg-6462_st1 6.46 5.47 5.775.41 6.08 5.66 4.70 5.20 hsa-asg-8485 hsa-asg-8485_st1 5.83 4.47 4.904.99 5.20 4.80 4.19 4.57 hsa-asg-11764 hsa-asg-11764_st1 6.23 5.01 5.505.77 5.81 4.94 4.70 5.14 hsa-miR-222 hsa-miR-222_st2 10.72 10.40 9.529.55 10.21 9.72 9.28 9.50 LNneg MicroRNA 8 9 M SD % ttest log2Diff Foldhsa-miR-124a 3.40 4.82 3.54 0.87 0 3.46E−06 10.3 1307 hsa-miR-124a 3.614.37 3.81 0.77 0 1.82E−06 9.95 986 hsa-asg-13159 2.05 3.84 3.18 0.70 06.44E−07 10.3 1270 hsa-miR-433 1.66 2.89 2.80 1.00 0 8.60E−05 7.70 207.8hsa-asg-13404 1.09 2.29 2.82 1.29 0 5.95E−04 7.44 173.3 hsa-miR-383 2.581.36 2.46 0.71 0 1.04E−05 7.24 151.3 hsa-asg-3373 1.85 1.83 2.78 0.96 01.08E−04 7.10 137.1 hsa-miR-129 4.55 4.37 4.57 0.40 0 1.90E−07 6.90119.7 hsa-miR-487b 3.71 4.37 5.58 1.11 67 3.79E−04 6.86 115.9 hsa-miR2022.41 3.06 3.45 0.88 0 7.68E−05 6.83 114.0 hsa-miR-323 2.89 3.22 3.900.98 0 1.68E−04 6.82 112.8 hsa-miR-329 2.74 3.06 3.16 0.84 0 7.69E−056.56 94.6 hsa-miR-329 3.29 2.71 3.41 0.74 0 3.02E−05 6.53 92.7hsa-miR-329 2.58 3.50 3.58 0.59 0 8.74E−06 6.23 75.2 hsa-miR-485-3p 2.743.06 3.24 0.66 0 1.36E−05 6.53 92.4 hsa-miR-485-3p 3.51 3.84 3.74 0.51 04.24E−06 5.94 61.4 hsa-miR-539 2.58 1.36 2.52 0.63 0 1.03E−05 6.49 90.1hsa-miR-539 1.66 2.07 2.19 0.63 0 1.38E−05 6.23 75.0 hsa-miR-9 3.29 4.133.29 0.55 0 3.88E−06 6.42 85.4 hsa-miR-9 1.66 3.62 2.79 0.63 0 2.86E−055.60 48.5 hsa-cand26 4.04 5.44 4.98 0.91 22 1.59E−04 6.39 83.8hsa-miR-410 1.85 2.51 2.51 0.68 0 2.03E−05 6.39 83.7 hsa-miR-149 3.353.14 3.04 1.03 0 3.80E−04 6.35 81.7 hsa-asg-13135 4.44 5.57 5.11 0.44 227.39E−07 6.35 81.6 hsa-asg-4574 3.29 3.06 3.38 0.63 0 1.17E−05 6.35 81.4hsa-miR-495 2.41 4.87 5.23 1.36 56 2.41E−03 6.26 76.4 hsa-miR-485-5p1.85 3.50 3.08 0.68 0 2.64E−05 6.14 70.5 hsa-miR-432 4.25 2.71 4.80 1.1322 9.21E−04 6.08 67.5 hsa-miR-487a 2.23 2.89 2.50 0.73 0 5.28E−05 6.0164.6 hsa-miR-487a 3.71 3.62 3.72 0.20 0 9.79E−09 5.08 33.9 hsa-asg-108815.01 5.20 5.19 0.55 22 8.15E−06 5.82 56.6 hsa-miR-338 3.40 4.87 4.301.04 11 7.55E−04 5.80 55.6 hsa-miR-137 3.29 2.89 2.68 0.56 0 1.07E−055.69 51.8 hsa-miR-346 4.63 3.14 3.27 0.88 0 3.28E−04 5.58 48.0hsa-asg-7295 2.74 1.83 2.43 0.57 0 1.56E−05 5.56 47.2 hsa-miR-411 2.232.07 3.83 1.27 11 3.31E−03 5.54 46.6 hsa-miR-592 2.58 4.51 2.94 0.96 06.38E−04 5.46 44.0 hsa-miR-592 1.66 5.40 2.89 1.21 11 2.99E−03 5.35 40.9hsa-miR-330 5.03 4.48 5.22 0.59 22 2.57E−05 5.39 42.0 hsa-miR-330 5.444.17 5.27 0.57 0 2.37E−05 5.23 37.4 hsa-asg-13166 2.05 4.04 3.94 0.88 04.39E−04 5.35 40.7 hsa-asg-5617 1.46 1.60 2.78 1.03 0 1.24E−03 5.27 38.6hsa-asg-4201 2.05 1.60 1.95 0.43 0 2.54E−06 5.27 38.5 hsa-asg-7472 0.920.93 1.97 0.85 0 4.08E−04 5.17 36.1 hsa-miR-299-5p 2.41 3.94 4.74 1.4044 8.03E−03 5.17 36.1 hsa-miR-328 4.66 3.73 4.96 1.94 0 3.51E−02 5.1736.0 hsa-asg-10202 3.35 1.71 3.09 1.22 0 3.91E−03 5.17 36.0 hsa-asg-39853.03 2.29 2.07 0.68 0 9.34E−05 5.16 35.7 hsa-miR-127 5.47 5.42 6.51 1.0356 1.57E−03 5.10 34.4 hsa-miR-380-3p 0.76 1.83 1.57 0.67 0 9.91E−05 5.0332.7 hsa-miR-380-3p 1.28 2.07 2.00 0.72 0 3.46E−04 4.52 22.9hsa-asg-14178 3.17 2.29 1.86 1.14 0 3.07E−03 5.03 32.6 hsa-miR-9* 4.715.20 5.55 0.53 44 1.98E−05 5.03 32.6 hsa-asg-13284 3.75 2.40 3.87 1.29 06.51E−03 4.95 30.8 hsa-miR-379 6.13 6.20 6.58 0.37 100 1.39E−06 4.9330.5 hsa-miR-138 4.87 5.09 5.62 0.80 33 4.11E−04 4.88 29.4 hsa-cand2061.28 2.71 2.65 0.86 0 6.69E−04 4.86 29.0 hsa-miR-584 5.88 5.82 5.85 0.3167 4.79E−07 4.75 27.0 hsa-miR-132 8.42 6.86 8.31 0.74 89 2.88E−04 4.7526.9 hsa-miR-382 5.91 5.79 6.70 0.61 78 9.77E−05 4.62 24.5 hsa-miR-154*3.17 4.37 4.31 0.79 11 5.45E−04 4.61 24.5 hsa-miR-598 3.03 5.02 5.611.12 56 4.63E−03 4.59 24.1 hsa-miR-212 4.95 4.73 5.36 0.61 11 1.06E−044.57 23.8 hsa-miR-153 1.46 2.07 2.00 0.52 0 3.46E−05 4.56 23.5hsa-miR-139 7.34 5.90 8.84 1.48 100 2.10E−02 4.47 22.2 hsa-miR-381 1.283.22 3.08 0.91 0 1.98E−03 4.35 20.4 hsa-miR-409-3p 2.58 3.73 3.75 1.06 04.66E−03 4.32 20.0 hsa-asg-9757 2.05 2.29 2.09 0.49 0 2.99E−05 4.31 19.8hsa-asg-2364 1.46 1.36 1.45 0.62 0 1.72E−04 4.29 19.6 hsa-asg-5021 3.032.07 4.63 1.58 22 3.54E−02 4.21 18.5 hsa-asg-4471 2.41 2.89 2.63 0.43 01.42E−05 4.19 18.2 hsa-miR-204 5.40 5.76 6.18 1.00 44 4.11E−03 4.18 18.2hsa-miR-128a 8.75 8.66 9.33 0.70 100 4.82E−04 4.15 17.8 hsa-miR-105 2.232.71 1.99 0.71 0 5.42E−04 4.15 17.8 hsa-asg-13254 2.41 3.36 4.86 1.37 562.17E−02 4.11 17.3 hsa-asg-10304 3.40 3.22 2.26 0.68 0 4.62E−04 4.0917.1 hsa-cand660 2.58 2.71 2.12 0.58 0 1.61E−04 4.06 16.7 hsa-miR-369-5p2.74 3.50 3.36 0.56 0 1.30E−04 4.05 16.6 hsa-miR-369-5p 2.58 3.50 3.420.81 0 2.22E−03 3.75 13.5 hsa-asg-12824 2.23 1.83 2.16 0.48 0 4.21E−054.04 16.4 hsa-asg-924 2.74 3.50 3.38 0.89 0 2.73E−03 4.03 16.3hsa-asg-9303 2.58 1.36 2.18 0.74 0 8.86E−04 4.01 16.1 hsa-miR-128b 9.189.23 9.75 0.70 100 7.64E−04 3.88 14.7 hsa-miR-184 3.80 4.13 4.10 1.09 119.85E−03 3.87 14.6 hsa-miR-370 4.47 5.09 4.84 0.36 0 6.89E−06 3.86 14.5hsa-asg-7465 5.20 5.02 4.86 0.66 11 5.99E−04 3.83 14.2 hsa-asg-9365 2.742.07 2.47 0.43 0 3.26E−05 3.80 13.9 hsa-asg-6667 1.85 1.36 1.76 0.58 02.57E−04 3.78 13.8 hsa-miR-491 5.48 5.44 5.47 0.33 0 4.37E−06 3.77 13.6hsa-miR-134 4.29 5.00 5.16 0.68 0 8.41E−04 3.74 13.4 hsa-miR-154 1.283.94 3.47 1.10 0 1.27E−02 3.72 13.1 hsa-asg-9514 2.58 3.36 3.43 0.79 02.04E−03 3.71 13.1 hsa-miR-187 3.35 3.14 3.33 0.61 0 4.69E−04 3.67 12.7hsa-miR-409-5p 5.43 5.24 5.63 0.58 44 3.34E−04 3.66 12.7 hsa-asg-54552.74 1.83 2.20 0.68 0 9.61E−04 3.63 12.4 hsa-miR-376a 3.80 3.73 4.341.02 0 9.77E−03 3.62 12.3 hsa-miR-376a 4.04 1.83 3.57 0.86 0 4.15E−033.61 12.2 hsa-miR-376a 4.12 4.64 4.83 0.75 11 2.24E−03 3.50 11.3hsa-asg-14020 1.76 3.06 1.91 0.73 0 1.51E−03 3.61 12.2 hsa-asg-2637 3.844.33 4.13 0.52 0 1.98E−04 3.54 11.6 hsa-asg-9971 4.12 3.62 3.68 0.46 01.05E−04 3.43 10.8 hsa-miR-7 8.56 8.46 8.60 0.64 100 9.79E−04 3.40 10.6hsa-miR-577 2.05 3.22 2.78 0.92 0 8.63E−03 3.37 10.3 hsa-asg-12250 2.052.07 2.05 0.35 0 1.80E−05 3.35 10.2 hsa-asg-10106 2.58 2.51 2.20 0.61 08.32E−04 3.32 10.0 hsa-asg-10439 2.05 2.89 2.15 0.46 0 1.28E−04 3.31 9.9hsa-asg-14176 4.19 4.13 5.46 0.88 44 7.19E−03 3.31 9.9 hsa-asg-109162.23 1.83 2.35 0.41 0 5.85E−05 3.29 9.8 hsa-asg-11880 1.28 2.07 2.450.67 0 1.83E−03 3.24 9.5 hsa-miR-181d 5.79 5.51 6.00 0.52 67 3.90E−043.23 9.4 hsa-asg-8067 5.28 5.38 5.97 0.60 33 1.03E−03 3.17 9.0hsa-miR-299-3p 3.17 3.50 2.75 0.78 0 5.13E−03 3.15 8.9 hsa-miR-299-3p1.85 2.89 2.91 0.52 0 6.22E−04 3.00 8.0 hsa-asg-12177 3.51 3.06 3.650.65 0 1.86E−03 3.14 8.8 hsa-asg-13496 3.03 3.06 3.04 0.36 0 3.42E−053.11 8.6 hsa-asg-14159 2.89 4.37 3.28 0.67 0 2.28E−03 3.10 8.6hsa-miR-499 4.55 3.84 4.87 0.85 22 9.02E−03 3.08 8.5 hsa-miR-499 4.254.58 4.86 0.74 22 6.98E−03 2.81 7.0 hsa-cand165 3.80 3.84 3.64 0.84 08.36E−03 3.06 8.3 hsa-miR-628 7.64 7.43 7.60 0.37 100 5.28E−05 3.06 8.3hsa-asg-2261 3.03 3.36 3.00 0.59 0 1.22E−03 3.06 8.3 hsa-miR-494 5.846.58 6.68 0.64 100 2.30E−03 2.97 7.9 hsa-miR-494 5.77 6.87 6.45 0.55 892.94E−03 2.45 5.5 hsa-miR-340 3.97 2.71 4.68 0.95 11 1.82E−02 2.97 7.8hsa-miR-504 3.56 5.26 4.46 1.11 0 3.67E−02 2.92 7.6 hsa-miR-181d 8.208.00 8.30 0.56 100 1.15E−03 2.91 7.5 hsa-miR-181d 8.15 7.86 8.36 0.44100 7.47E−04 2.43 5.4 hsa-asg-5083 2.58 2.07 2.42 0.25 0 4.77E−06 2.897.4 hsa-miR-189 3.51 4.92 5.33 1.09 44 3.64E−02 2.88 7.4 hsa-asg-110402.23 2.07 2.69 0.48 0 4.74E−04 2.87 7.3 hsa-asg-1388 2.41 3.50 2.75 0.640 3.03E−03 2.84 7.2 hsa-asg-13258 6.22 7.92 7.82 0.94 100 2.18E−02 2.827.1 hsa-asg-13646 6.05 7.10 7.23 0.76 100 8.27E−03 2.80 7.0hsa-asg-13231 2.05 4.44 3.47 1.01 0 3.28E−02 2.75 6.7 hsa-asg-11949 4.444.87 4.16 0.57 0 1.93E−03 2.73 6.6 hsa-asg-14175 5.91 6.23 6.25 0.42 893.52E−04 2.64 6.2 hsa-asg-12515 4.12 3.73 3.98 0.45 0 6.04E−04 2.58 6.0hsa-miR-331 5.44 5.91 6.44 1.04 56 4.71E−02 2.57 5.9 hsa-miR-331 7.267.18 7.83 0.72 100 1.08E−02 2.51 5.7 hsa-asg-4283 2.23 2.51 3.19 0.65 06.25E−03 2.52 5.8 hsa-miR-181b 10.46 10.1 10.4 0.45 100 7.18E−04 2.525.7 hsa-cand208 3.97 2.51 3.03 0.66 0 7.09E−03 2.48 5.6 hsa-miR-218 7.438.74 8.77 0.77 100 1.63E−02 2.46 5.5 hsa-asg-13878 2.41 1.83 3.21 0.90 03.38E−02 2.43 5.4 hsa-miR-551b 3.51 4.29 4.35 0.82 22 2.34E−02 2.42 5.3hsa-miR-551b 2.89 4.37 4.14 0.84 0 4.03E−02 2.16 4.5 hsa-asg-4553 3.803.73 3.51 0.58 0 4.34E−03 2.40 5.3 hsa-miR-181a* 5.77 6.53 5.80 0.43 568.02E−04 2.39 5.2 hsa-asg-3376 4.19 3.36 3.82 0.77 0 1.90E−02 2.39 5.2hsa-asg-2335 2.23 2.89 3.11 0.66 0 9.37E−03 2.37 5.2 hsa-asg-4439 3.714.04 3.31 0.84 0 2.86E−02 2.36 5.1 hsa-miR-421 8.76 8.05 8.19 0.36 1002.57E−04 2.36 5.1 hsa-miR-421 9.37 8.90 9.02 0.38 100 5.16E−04 2.23 4.7hsa-miR-31 8.66 10.0 9.21 0.92 100 4.03E−02 2.36 5.1 hsa-cand244 4.633.89 4.70 0.48 0 1.67E−03 2.32 5.0 hsa-asg-12164 4.12 2.89 3.91 0.91 04.29E−02 2.31 4.9 hsa-miR-324-5p 7.85 7.97 7.93 0.29 100 6.99E−05 2.274.8 hsa-asg-8476 5.40 5.61 6.04 0.47 11 1.95E−03 2.23 4.7 hsa-miR-6544.19 3.62 4.55 0.89 11 4.64E−02 2.20 4.6 hsa-asg-8776 3.51 3.22 3.450.61 0 9.13E−03 2.19 4.6 hsa-asg-8266 3.40 3.84 4.29 0.55 0 7.51E−032.07 4.2 hsa-asg-879 2.74 3.84 3.42 0.55 0 7.00E−03 2.07 4.2 hsa-cand3303.97 3.94 4.19 0.80 11 4.54E−02 1.99 4.0 hsa-asg-12183 3.97 4.21 3.940.78 0 4.35E−02 1.97 3.9 hsa-miR-181c 8.69 9.81 8.90 0.61 100 1.72E−021.92 3.8 hsa-asg-13279 6.98 6.66 7.22 0.52 100 9.24E−03 1.86 3.6hsa-asg-4294 4.19 3.06 3.53 0.75 0 4.92E−02 1.84 3.6 hsa-cand443 3.804.29 4.59 0.68 33 3.45E−02 1.82 3.5 hsa-asg-191 3.17 4.82 3.71 0.58 01.95E−02 1.78 3.4 hsa-asg-14138 3.93 4.54 4.87 0.64 0 3.00E−02 1.77 3.4hsa-miR-99b 9.51 10.3 10.5 0.60 100 2.35E−02 1.76 3.4 hsa-miR-99b 9.5310.5 10.6 0.65 100 4.17E−02 1.67 3.2 hsa-asg-4493 3.80 3.62 3.65 0.56 01.76E−02 1.75 3.4 hsa-asg-12179 5.01 5.32 5.29 0.43 11 6.02E−03 1.69 3.2hsa-asg-1467 4.71 4.64 5.00 0.65 11 4.04E−02 1.68 3.2 hsa-asg-9646 3.403.73 3.82 0.43 0 6.43E−03 1.66 3.2 hsa-asg-13293 3.88 3.36 3.77 0.49 01.29E−02 1.66 3.2 hsa-miR-425-3p 6.24 5.26 6.24 0.49 89 1.33E−02 1.633.1 hsa-asg-3145 4.44 5.32 5.44 0.61 33 3.65E−02 1.60 3.0 hsa-asg-34053.29 4.13 3.87 0.33 0 1.87E−03 1.59 3.0 hsa-asg-6543 3.71 3.36 3.88 0.360 3.06E−03 1.58 3.0 hsa-asg-13471 4.04 3.50 4.61 0.61 11 4.08E−02 1.573.0 hsa-asg-11199 10.45 11.4 11.5 0.58 100 4.17E−02 1.48 2.8hsa-asg-6050 4.32 3.73 3.93 0.41 0 9.66E−03 1.47 2.8 hsa-miR-376a* 3.974.21 4.46 0.53 0 3.05E−02 1.46 2.7 hsa-miR-98 10.16 10.6 10.9 0.44 1001.72E−02 1.39 2.6 hsa-asg-7507 4.89 5.40 4.54 0.51 0 3.45E−02 1.37 2.6hsa-miR-185 11.24 10.2 10.6 0.47 100 2.84E−02 1.32 2.5 hsa-asg-2460 4.754.76 4.45 0.42 0 1.97E−02 1.29 2.4 hsa-asg-5134 3.80 4.44 4.54 0.38 01.26E−02 1.29 2.4 hsa-miR-181a 12.10 12.5 12.0 0.43 100 2.72E−02 1.232.3 hsa-asg-4438 4.80 5.32 4.61 0.39 0 1.90E−02 1.20 2.3 hsa-let-7e11.33 11.8 12.2 0.48 100 4.48E−02 1.20 2.3 hsa-asg-10626 4.25 4.97 4.840.39 0 2.07E−02 1.18 2.3 hsa-miR-324-3p 6.63 7.25 7.33 0.42 0 3.01E−021.17 2.3 hsa-asg-7335 5.09 5.28 5.10 0.34 22 1.18E−02 1.15 2.2hsa-asg-917 4.85 5.20 5.13 0.43 33 3.52E−02 1.13 2.2 hsa-asg-12275 6.436.56 6.31 0.33 100 1.44E−02 1.07 2.1 hsa-asg-6462 4.80 5.40 5.39 0.44 445.00E−02 1.07 2.1 hsa-asg-8485 5.13 4.87 4.79 0.33 0 1.63E−02 1.04 2.1hsa-asg-11764 5.13 4.76 5.20 0.41 22 4.44E−02 1.04 2.0 hsa-miR-222 9.379.84 9.71 0.38 100 3.53E−02 1.01 2.0 SD, standard deviation; %,percentage of samples expressing miRNA above background levels;Log2Diff, difference in VSN-transformed expression bewteen BRN and LNnegsamples.

Example 35 miRNAs Differentally Expressed Between Normal Thymus Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous thymus cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal thymustissue than in cancer-negative lymph nodes.

Total RNA, purified from one normal human thymus sample (FirstChoice®Human Thymus Total RNA, cat. no. AM7964; Ambion, Austin, Tex., USA) andfrom nine normal human lymph node samples (FirstChoice® Human Lymph NodeTotal RNA, cat. no. AM7894; Ambion, Austin, Tex., USA) was purchasedfrom Ambion. miRNA purification and labeling and microarray expressionanalyses were performed as described above in Example 2.

The inventors identified 108 miRNAs (44 verified, 64 predicted) withsignificant differential expression between the normal thymus and normallymph node samples (|log 2Diff|thymus vs LN neg≧1 and p-value<0.05)(Table 34). Among the verified miRNAs, hsa-miR-205 was expressed at alevel over 300-fold higher in the thymus sample; hsa-miR-383 and -429were expressed at levels between 50- and 70-fold higher in thymus; 15miRNAs were expressed at levels between 10- and 40-fold higher inthymus; and 15 miRNAs were expressed at levels between 5- and 10-foldhigher in the thymus sample than in normal lymph nodes. Among thepredicted miRNAs, hsa-asg-14153_st2 was expressed at a level over50-fold higher in the thymus sample; 6 miRNAs (hsa-miR-7637_st2, -13404st2, -1088_st1, -11715_st2, -3711 st2, and hsa-miR202_st2) wereexpressed at levels between 10- and 30-fold higher in thymus; and 17miRNAs were expressed at levels between 5- and 10-fold higher in thethymus samples than in normal lymph nodes.

TABLE 34 MicroRNAs Differentially Expressed Between Normal ThymusSamples (TMS) and Cancer-Negative Lymph Node Samples (LNneg) fromPatients. Probe LNneg MicroRNA ID TMS 1 2 3 4 5 6 hsa-miR-205hsa-miR-205_st1 12.96 2.90 5.86 5.64 2.00 3.55 6.06 hsa-miR-383hsa-miR-383_st1 8.51 3.31 2.83 2.40 2.57 3.18 1.30 hsa-miR-429hsa-miR-429_st1 8.74 3.42 1.54 3.66 2.10 2.33 2.65 hsa-asg-14153hsa-asg-14153_st2 8.51 3.05 2.23 3.04 2.98 2.33 2.65 hsa-miR-200ahsa-miR-200a_st2 10.75 6.26 5.02 6.49 5.71 5.25 4.54 hsa-miR-200bhsa-miR-200b_st1 12.84 7.61 7.75 8.91 6.69 6.79 6.82 hsa-miR-183hsa-miR-183_st1 11.95 7.65 6.71 6.71 7.93 7.54 5.78 hsa-miR-96hsa-miR-96_st1 8.13 4.97 3.36 3.04 2.98 4.07 2.80 hsa-asg-7637hsa-asg-7637_st2 8.66 3.64 2.50 4.46 3.44 3.89 3.97 hsa-miR-375hsa-miR-375_st1 8.79 5.95 4.88 6.24 2.82 2.93 3.63 hsa-asg-13404hsa-asg-13404_st2 7.28 4.15 2.67 5.28 2.90 3.24 1.57 hsa-miR-203hsa-miR-203_st2 11.16 7.32 5.84 7.22 8.24 6.42 5.36 hsa-miR-495hsa-miR-495_st1 9.49 6.01 4.76 6.97 6.25 6.29 4.38 hsa-miR-200chsa-miR-200c_st1 13.29 9.59 8.79 10.31 8.17 8.12 8.08 hsa-miR-149hsa-miR-149_st2 7.17 4.47 3.82 1.76 4.14 2.72 1.48 hsa-miR-493-5phsa-miR-493-5p_st1 8.74 4.35 3.78 6.89 6.18 6.30 3.63 hsa-asg-1088hsa-asg-1088_st1 5.88 1.83 2.13 1.63 1.59 2.07 0.87 hsa-asg-11715hsa-asg-11715_st2 5.84 1.83 2.32 2.15 1.48 2.07 1.76 hsa-miR202hsa-miR202_st2 7.36 3.83 2.67 4.93 3.94 3.59 2.40 hsa-miR-411hsa-miR-411_st1 7.69 4.84 3.68 4.54 5.22 5.52 2.94 hsa-miR-487bhsa-miR-487b_st2 9.32 6.05 5.79 6.97 6.90 6.22 5.34 hsa-miR-182hsa-miR-182_st1 12.99 10.22 9.98 8.86 9.82 9.35 9.50 hsa-miR-368hsa-miR-368_st2 9.77 6.48 6.47 7.70 8.08 7.68 5.18 hsa-asg-3711hsa-asg-3711_st2 8.65 5.69 6.12 5.12 5.52 5.11 5.20 hsa-miR-605hsa-miR-605_st1 5.97 2.58 2.13 3.04 0.73 1.71 2.14 hsa-asg-10247hsa-asg-10247_st1 5.79 2.75 2.13 4.01 1.69 2.16 2.32 hsa-miR-432hsa-miR-432_st2 8.02 4.53 5.38 5.93 5.52 5.74 5.62 hsa-asg-12139hsa-asg-12139_st2 5.50 2.75 2.32 2.63 2.30 1.62 2.14 hsa-asg-3807hsa-asg-3807_st1 6.33 3.05 3.36 3.04 3.94 3.85 1.67 hsa-miR-200a*hsa-miR-200a-_st2 5.83 3.05 2.83 2.15 3.89 2.57 1.76 hsa-asg-8054hsa-asg-8054_st1 5.27 3.42 2.32 1.37 1.69 2.33 1.21 hsa-miR-376ahsa-asg-10049_st1 7.79 5.01 4.85 6.09 5.55 5.31 4.05 hsa-miR-376ahsa-miR-376a_st2 7.09 5.13 4.03 5.49 5.66 4.82 3.97 hsa-miR-376ahsa-asg-14234_st1 6.06 3.31 4.25 3.38 3.98 4.82 3.63 hsa-asg-5119hsa-asg-5119_st1 5.45 2.41 2.67 1.89 1.90 1.98 2.49 hsa-asg-13908hsa-asg-13908_st2 5.56 2.03 2.50 3.66 2.48 1.44 2.80 hsa-miR-433hsa-miR-433_st2 5.67 2.41 2.67 4.54 2.90 4.17 2.32 hsa-miR-485-3phsa-miR23_st2 6.09 4.29 2.50 4.11 3.25 2.41 3.20 hsa-miR-485-3phsa-miR-485-3p_st2 6.06 3.64 2.97 4.30 4.41 4.24 3.08 hsa-asg-9980hsa-asg-9980_st1 6.74 5.17 4.44 4.01 1.69 3.35 2.80 hsa-asg-5721hsa-asg-5721_st2 5.33 2.22 3.36 2.15 3.12 1.98 2.32 hsa-miR-369-5phsa-miR-369-5p_st2 6.21 3.53 2.67 4.46 4.35 4.33 2.49 hsa-miR-369-5phsa-asg-1521_st1 5.92 3.73 3.36 4.01 2.20 3.68 3.68 hsa-asg-4978hsa-asg-4978_st2 5.27 1.63 2.83 2.84 1.69 1.98 1.95 hsa-miR-539hsa-miR-539_st1 5.30 2.03 3.11 2.63 3.38 2.65 2.94 hsa-miR-127hsa-miR-127_st1 9.26 6.80 5.52 7.96 7.53 7.66 5.66 hsa-asg-13705hsa-asg-13705_st1 5.01 1.63 0.97 3.78 2.30 2.65 2.32 hsa-asg-8428hsa-asg-8428_st2 4.52 2.22 1.94 2.63 2.00 2.49 1.03 hsa-miR-485-5phsa-miR-485-5p_st2 5.79 3.42 3.24 3.38 3.66 3.24 1.95 hsa-miR-128ahsa-miR-128a_st2 12.00 10.77 9.31 9.72 9.95 8.83 8.94 hsa-miR-410hsa-miR-410_st1 5.13 2.41 2.32 1.37 3.71 2.49 2.80 hsa-miR-128bhsa-miR-128b_st1 12.36 11.26 9.78 10.01 10.3 9.17 9.27 hsa-asg-10131hsa-asg-10131_st2 5.81 3.31 3.11 3.52 2.48 2.49 3.20 hsa-asg-13166hsa-asg-13166_st2 6.53 4.47 3.36 4.87 4.94 4.07 4.12 hsa-miR-141hsa-miR-141_st1 6.71 5.05 3.78 4.93 4.41 4.36 2.80 hsa-asg-13997hsa-asg-13997_st2 5.04 3.05 3.24 2.15 2.98 2.16 2.32 hsa-asg-8174hsa-asg-8174_st1 5.73 3.31 4.03 2.84 2.98 2.86 3.20 hsa-miR-379hsa-miR-379_st1 9.04 6.51 6.37 7.12 6.96 7.01 6.54 hsa-asg-3912hsa-asg-3912_st1 4.73 1.63 2.50 2.84 1.59 2.16 1.21 hsa-asg-4219hsa-asg-4219_st1 4.87 3.42 1.74 1.63 1.48 2.24 1.76 hsa-miR-329hsa-miR-329_st1 5.84 3.73 2.97 4.61 3.25 4.54 2.80 hsa-miR-329hsa-asg-13204_st2 5.63 4.07 2.83 4.21 3.38 4.20 3.43 hsa-cand206hsa-cand206_st1 4.94 2.22 1.94 3.78 3.60 2.65 2.14 hsa-asg-1296hsa-asg-1296_st1 5.52 3.05 2.50 4.81 2.39 2.49 2.73 hsa-asg-924hsa-asg-924_st1 5.65 4.64 4.25 3.38 4.44 2.65 2.73 hsa-asg-10306hsa-asg-10306_st1 5.07 2.22 2.32 3.52 2.74 3.40 2.73 hsa-asg-11160hsa-asg-11160_st2 5.38 2.75 3.11 3.38 3.05 2.86 2.65 hsa-cand696hsa-cand696_st1 5.19 3.53 3.11 2.84 2.98 2.49 1.95 hsa-miR-382hsa-miR-382_st1 8.84 7.16 6.80 7.35 7.13 7.40 6.51 hsa-asg-1358hsa-asg-1358_st1 5.01 3.18 3.11 3.38 2.30 2.33 3.20 hsa-asg-1596hsa-asg-1596_st2 6.33 4.41 5.06 4.01 3.19 4.36 4.12 hsa-asg-4294hsa-asg-4294_st2 5.65 4.29 3.11 4.54 4.06 3.18 3.08 hsa-asg-12880hsa-asg-12880_st1 8.95 8.00 6.95 5.74 6.87 7.96 6.98 hsa-miR-132hsa-miR-132_st1 10.29 9.71 8.51 8.34 8.34 8.56 8.02 hsa-miR-154*hsa-miR-154-_st2 6.28 5.81 3.48 4.21 4.98 4.59 3.89 hsa-asg-758hsa-asg-758_st1 7.97 6.45 6.59 5.07 6.13 7.04 6.24 hsa-miR-134hsa-miR-134_st2 7.08 5.09 5.73 6.00 5.84 5.67 4.15 hsa-miR-181dhsa-miR-181d_st1 10.07 9.12 7.78 8.91 8.92 7.57 7.91 hsa-miR-181dhsa-miR74_st1 9.64 8.96 8.17 9.00 8.79 7.99 8.20 hsa-miR-181bhsa-miR-181b_st2 12.23 11.22 10.4 10.98 10.8 9.81 10.2 hsa-asg-12177hsa-asg-12177_st2 5.35 4.74 3.36 3.52 4.62 3.06 3.93 hsa-miR-181a*hsa-miR-213_st1 7.50 6.29 6.17 5.68 5.71 5.42 5.37 hsa-asg-8515hsa-asg-8515_st2 9.39 8.26 7.40 7.32 6.91 8.54 6.77 hsa-asg-11361hsa-asg-11361_st2 5.04 2.75 3.48 3.38 3.25 3.59 3.32 hsa-asg-5326hsa-asg-5326_st2 7.94 6.95 7.13 5.74 6.26 6.65 6.47 hsa-asg-1306hsa-asg-1306_st2 5.92 4.07 4.58 3.72 3.94 4.86 3.81 hsa-cand914hsa-cand914_st1 11.84 11.06 10.8 9.59 10.0 10.84 10.2 hsa-asg-13598hsa-asg-13598_st1 7.16 5.58 6.43 4.96 5.53 6.03 6.27 hsa-asg-7578hsa-asg-7578_st2 6.84 6.37 5.23 4.16 5.28 5.82 5.04 hsa-miR-212hsa-miR-212_st2 6.94 6.26 5.68 6.18 5.71 5.14 5.05 hsa-asg-10638hsa-asg-10638_st2 10.27 9.24 9.20 7.43 8.68 9.23 9.03 hsa-asg-872hsa-asg-872_st2 8.23 7.32 7.06 5.45 7.16 6.76 6.81 hsa-asg-10731hsa-asg-10731_st1 5.50 4.53 3.78 3.66 3.75 4.86 3.58 hsa-asg-6883hsa-asg-6883_st1 5.73 4.59 4.18 5.09 4.44 3.59 4.35 hsa-asg-4663hsa-asg-4663_st1 9.17 7.99 8.01 6.67 7.47 8.39 7.64 hsa-asg-5444hsa-asg-5444_st2 5.67 4.07 5.02 3.52 4.17 4.04 5.07 hsa-cand533hsa-cand533_st1 5.47 4.84 4.18 4.11 4.48 3.85 3.53 hsa-miR-566hsa-miR-566_st1 7.21 6.72 6.12 5.12 5.83 6.11 6.10 hsa-asg-3729hsa-asg-3729_st2 8.45 7.38 7.23 6.88 6.83 7.81 6.82 hsa-asg-8568hsa-asg-8568_st1 10.93 10.10 10.1 9.53 9.55 10.29 10.0 hsa-miR-487ahsa-asg-6915_st1 4.91 4.07 3.58 3.90 3.38 3.85 3.72 hsa-asg-3130hsa-asg-3130_st1 9.72 9.01 8.29 8.09 8.36 9.14 8.29 hsa-asg-11729hsa-asg-11729_st2 9.24 8.50 8.40 8.13 7.84 8.47 7.83 hsa-asg-10892hsa-asg-10892_st1 9.65 8.85 8.57 8.04 8.11 9.12 8.50 hsa-asg-9874hsa-asg-9874_st1 11.38 10.31 10.5 9.69 9.95 10.92 10.1 hsa-asg-12179hsa-asg-12179_st1 6.40 6.15 4.98 5.45 5.67 5.36 4.88 hsa-miR-652hsa-miR-652_st1 10.56 9.77 9.23 9.20 9.98 9.11 9.01 hsa-asg-4246hsa-asg-4246_st2 5.93 4.59 5.41 4.75 4.87 5.17 4.92 hsa-asg-13604hsa-asg-13604_st1 9.69 8.97 8.88 8.07 8.16 9.26 8.81 hsa-asg-8068hsa-asg-8068_st2 7.28 6.73 6.65 5.59 5.96 6.42 6.58 hsa-asg-3722hsa-asg-3722_st1 9.93 9.12 9.51 9.24 8.61 8.85 8.99 hsa-asg-3038hsa-asg-3038_st2 6.66 5.84 5.79 5.45 5.90 5.89 5.94 LNneg MicroRNA 7 8 9M SD % ttest log2Diff Fold hsa-miR-205 3.38 5.30 7.74 4.71 1.85 332.87E−03 −8.24 302 hsa-miR-383 2.62 2.58 1.36 2.46 0.71 0 3.86E−05 −6.0566.4 hsa-miR-429 2.31 2.41 5.36 2.86 1.14 0 1.20E−03 −5.88 58.8hsa-asg-14153 1.17 2.74 4.37 2.73 0.85 0 1.99E−04 −5.79 55.2hsa-miR-200a 4.05 4.75 7.76 5.54 1.15 67 2.60E−03 −5.21 37.0hsa-miR-200b 6.50 7.64 10.41 7.68 1.27 100 4.79E−03 −5.17 35.9hsa-miR-183 7.50 6.30 6.63 6.97 0.72 89 1.69E−04 −4.98 31.5 hsa-miR-963.03 1.85 3.06 3.24 0.87 11 6.82E−04 −4.89 29.6 hsa-asg-7637 3.27 3.615.24 3.78 0.77 0 3.16E−04 −4.88 29.4 hsa-miR-375 1.00 4.61 6.37 4.271.82 0 4.63E−02 −4.52 22.9 hsa-asg-13404 2.14 1.09 2.29 2.82 1.29 01.11E−02 −4.46 22.1 hsa-miR-203 5.17 6.05 9.34 6.77 1.38 78 1.68E−02−4.39 21.0 hsa-miR-495 5.14 2.41 4.87 5.23 1.36 56 1.80E−02 −4.26 19.1hsa-miR-200c 7.96 8.87 11.51 9.05 1.21 100 1.06E−02 −4.25 19.0hsa-miR-149 2.47 3.35 3.14 3.04 1.03 0 5.15E−03 −4.13 17.6hsa-miR-493-5p 4.36 1.85 4.58 4.66 1.58 33 3.95E−02 −4.08 16.9hsa-asg-1088 3.27 1.46 2.51 1.93 0.68 0 5.90E−04 −3.95 15.5hsa-asg-11715 0.63 1.46 3.36 1.90 0.74 0 9.89E−04 −3.95 15.4 hsa-miR2024.25 2.41 3.06 3.45 0.88 0 2.88E−03 −3.91 15.0 hsa-miR-411 3.38 2.232.07 3.83 1.27 11 2.05E−02 −3.87 14.6 hsa-miR-487b 4.88 3.71 4.37 5.581.11 67 1.28E−02 −3.73 13.3 hsa-miR-182 8.97 8.56 8.11 9.26 0.70 1009.59E−04 −3.72 13.2 hsa-miR-368 5.62 3.97 5.07 6.25 1.40 67 4.46E−02−3.52 11.5 hsa-asg-3711 4.52 4.41 4.84 5.17 0.55 0 3.13E−04 −3.48 11.2hsa-miR-605 2.90 3.03 4.44 2.52 1.03 0 1.33E−02 −3.44 10.9 hsa-asg-102470.37 3.17 3.94 2.50 1.14 0 2.54E−02 −3.28 9.7 hsa-miR-432 3.48 4.25 2.714.80 1.13 22 2.67E−02 −3.22 9.3 hsa-asg-12139 1.60 2.05 3.50 2.32 0.59 09.13E−04 −3.18 9.0 hsa-asg-3807 3.38 2.89 3.62 3.20 0.68 0 2.35E−03−3.13 8.8 hsa-miR-200a* 2.14 3.17 2.89 2.72 0.64 0 1.80E−03 −3.11 8.6hsa-asg-8054 3.03 2.05 2.51 2.21 0.73 0 4.13E−03 −3.06 8.3 hsa-miR-376a3.83 4.12 4.64 4.83 0.75 11 5.75E−03 −2.96 7.8 hsa-miR-376a 2.47 3.803.73 4.34 1.02 0 3.39E−02 −2.75 6.7 hsa-miR-376a 2.90 4.04 1.83 3.570.86 0 2.57E−02 −2.49 5.6 hsa-asg-5119 2.90 2.58 3.62 2.49 0.55 09.79E−04 −2.96 7.8 hsa-asg-13908 2.62 2.41 3.73 2.63 0.72 0 4.85E−03−2.93 7.6 hsa-miR-433 1.60 1.66 2.89 2.80 1.00 0 2.64E−02 −2.88 7.3hsa-miR-485-3p 3.57 2.74 3.06 3.24 0.66 0 3.41E−03 −2.86 7.2hsa-miR-485-3p 3.66 3.51 3.84 3.74 0.51 0 2.65E−03 −2.32 5.0hsa-asg-9980 3.83 5.05 4.64 3.89 1.13 11 4.34E−02 −2.85 7.2 hsa-asg-57211.78 2.89 2.51 2.48 0.54 0 1.01E−03 −2.84 7.2 hsa-miR-369-5p 2.90 2.583.50 3.42 0.81 0 1.11E−02 −2.79 6.9 hsa-miR-369-5p 3.38 2.74 3.50 3.360.56 0 2.57E−03 −2.55 5.9 hsa-asg-4978 2.77 2.58 4.13 2.49 0.79 09.89E−03 −2.78 6.9 hsa-miR-539 1.96 2.58 1.36 2.52 0.63 0 3.09E−03 −2.786.9 hsa-miR-127 6.57 5.47 5.42 6.51 1.03 56 3.53E−02 −2.75 6.7hsa-asg-13705 0.37 3.17 3.22 2.27 1.11 0 4.69E−02 −2.74 6.7 hsa-asg-84280.04 2.14 1.60 1.79 0.81 0 1.26E−02 −2.73 6.6 hsa-miR-485-5p 3.48 1.853.50 3.08 0.68 0 5.36E−03 −2.71 6.5 hsa-miR-128a 9.00 8.75 8.66 9.330.70 100 6.58E−03 −2.68 6.4 hsa-miR-410 3.15 1.85 2.51 2.51 0.68 06.52E−03 −2.62 6.2 hsa-miR-128b 9.53 9.18 9.23 9.75 0.70 100 7.67E−03−2.61 6.1 hsa-asg-10131 2.62 3.17 5.07 3.22 0.79 0 1.43E−02 −2.59 6.0hsa-asg-13166 3.57 2.05 4.04 3.94 0.88 0 2.43E−02 −2.58 6.0 hsa-miR-1413.83 3.80 4.51 4.16 0.69 0 8.27E−03 −2.54 5.8 hsa-asg-13997 1.25 3.172.29 2.51 0.65 0 6.17E−03 −2.53 5.8 hsa-asg-8174 2.77 2.74 4.13 3.210.53 0 1.95E−03 −2.52 5.8 hsa-miR-379 6.33 6.13 6.20 6.58 0.37 1002.22E−04 −2.46 5.5 hsa-asg-3912 2.90 2.14 3.50 2.27 0.74 0 1.33E−02−2.45 5.5 hsa-asg-4219 2.90 2.74 3.94 2.43 0.87 0 2.90E−02 −2.44 5.4hsa-miR-329 2.77 3.29 2.71 3.41 0.74 0 1.39E−02 −2.44 5.4 hsa-miR-3293.98 2.58 3.50 3.58 0.59 0 1.12E−02 −2.05 4.2 hsa-cand206 3.57 1.28 2.712.65 0.86 0 3.52E−02 −2.29 4.9 hsa-asg-1296 3.15 3.88 4.13 3.24 0.85 03.46E−02 −2.28 4.9 hsa-asg-924 2.14 2.74 3.50 3.38 0.89 0 4.30E−02 −2.274.8 hsa-asg-10306 3.38 2.05 3.06 2.82 0.55 0 4.67E−03 −2.25 4.8hsa-asg-11160 2.47 3.51 5.11 3.21 0.79 0 3.13E−02 −2.17 4.5 hsa-cand6963.48 3.80 3.22 3.05 0.57 0 7.09E−03 −2.15 4.4 hsa-miR-382 6.27 5.91 5.796.70 0.61 78 1.07E−02 −2.14 4.4 hsa-asg-1358 2.47 2.05 3.84 2.87 0.61 01.01E−02 −2.14 4.4 hsa-asg-1596 4.12 4.19 4.37 4.20 0.49 0 3.30E−03−2.13 4.4 hsa-asg-4294 2.31 4.19 3.06 3.53 0.75 0 2.84E−02 −2.12 4.3hsa-asg-12880 6.48 7.23 5.67 6.88 0.83 33 4.52E−02 −2.07 4.2 hsa-miR-1328.00 8.42 6.86 8.31 0.74 89 3.42E−02 −1.98 4.0 hsa-miR-154* 4.25 3.174.37 4.31 0.79 11 4.50E−02 −1.97 3.9 hsa-asg-758 5.41 6.52 4.79 6.030.76 67 4.19E−02 −1.94 3.8 hsa-miR-134 4.71 4.29 5.00 5.16 0.68 02.89E−02 −1.92 3.8 hsa-miR-181d 8.26 8.20 8.00 8.30 0.56 100 1.67E−02−1.78 3.4 hsa-miR-181d 8.08 8.15 7.86 8.36 0.44 100 2.34E−02 −1.29 2.4hsa-miR-181b 10.2 10.46 10.13 10.4 0.45 100 6.29E−03 −1.74 3.3hsa-asg-12177 3.03 3.51 3.06 3.65 0.65 0 3.85E−02 −1.70 3.3hsa-miR-181a* 5.30 5.77 6.53 5.80 0.43 56 5.97E−03 −1.70 3.2hsa-asg-8515 7.71 8.26 8.11 7.70 0.63 100 3.51E−02 −1.69 3.2hsa-asg-11361 3.83 2.58 4.29 3.39 0.52 0 1.62E−02 −1.66 3.1 hsa-asg-53265.52 6.55 5.32 6.29 0.63 67 3.82E−02 −1.65 3.1 hsa-asg-1306 4.18 5.414.17 4.31 0.55 0 2.34E−02 −1.62 3.1 hsa-cand914 9.80 10.58 9.10 10.20.65 100 4.72E−02 −1.62 3.1 hsa-asg-13598 4.31 5.47 5.38 5.55 0.66 224.85E−02 −1.61 3.1 hsa-asg-7578 5.14 5.55 4.67 5.25 0.64 11 4.62E−02−1.59 3.0 hsa-miR-212 4.57 4.95 4.73 5.36 0.61 11 4.13E−02 −1.57 3.0hsa-asg-10638 8.31 8.70 8.51 8.70 0.58 100 3.42E−02 −1.57 3.0hsa-asg-872 6.77 6.82 5.82 6.66 0.62 33 4.38E−02 −1.56 3.0 hsa-asg-107313.48 3.71 4.44 3.98 0.50 0 1.98E−02 −1.52 2.9 hsa-asg-6883 4.18 3.514.58 4.28 0.49 0 2.36E−02 −1.45 2.7 hsa-asg-4663 7.42 8.45 7.49 7.730.55 100 3.81E−02 −1.45 2.7 hsa-asg-5444 4.42 4.61 3.50 4.27 0.57 04.83E−02 −1.40 2.6 hsa-cand533 3.48 4.04 4.21 4.08 0.43 0 1.51E−02 −1.392.6 hsa-miR-566 5.55 6.02 5.42 5.89 0.47 56 2.85E−02 −1.33 2.5hsa-asg-3729 6.87 7.41 7.31 7.17 0.34 100 7.63E−03 −1.28 2.4hsa-asg-8568 9.12 9.67 8.93 9.71 0.47 100 3.96E−02 −1.22 2.3hsa-miR-487a 3.66 3.71 3.62 3.72 0.20 0 5.12E−04 −1.19 2.3 hsa-asg-31308.46 9.00 8.31 8.55 0.39 100 2.11E−02 −1.18 2.3 hsa-asg-11729 8.00 8.027.68 8.10 0.30 78 7.02E−03 −1.14 2.2 hsa-asg-10892 8.17 8.85 8.56 8.530.37 22 2.14E−02 −1.12 2.2 hsa-asg-9874 9.78 10.56 10.41 10.2 0.41 1003.09E−02 −1.12 2.2 hsa-asg-12179 4.80 5.01 5.32 5.29 0.43 11 4.18E−02−1.10 2.1 hsa-miR-652 9.63 9.50 9.64 9.45 0.33 100 1.32E−02 −1.10 2.1hsa-asg-4246 4.25 5.30 4.58 4.87 0.38 0 2.85E−02 −1.06 2.1 hsa-asg-136048.39 8.80 8.38 8.64 0.40 100 3.65E−02 −1.06 2.1 hsa-asg-8068 5.86 6.375.86 6.22 0.41 89 4.21E−02 −1.05 2.1 hsa-asg-3722 8.29 9.00 8.68 8.920.37 100 3.03E−02 −1.01 2.0 hsa-asg-3038 5.38 5.13 5.61 5.66 0.28 441.01E−02 −1.00 2.0 SD, standard deviation; %, percentage of samplesexpressing miRNA above background levels; Log2Diff, difference inVSN-transformed expression bewteen TMS and LNneg samples.

Example 36 miRNAs Differentally Expressed Between Normal Trachea Tissueand Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous trachea cells to lymph nodes, the inventors identified miRNAsthat are expressed at significantly higher levels in normal tracheatissue than in cancer-negative lymph nodes.

Total RNA, purified from one normal human trachea sample (FirstChoice®Human Trachea Total RNA, cat. no. AM6846; Ambion, Austin, Tex., USA) andfrom nine normal human lymph node samples (FirstChoice® Human Lymph NodeTotal RNA, cat. no. AM7894; Ambion, Austin, Tex., USA) was purchasedfrom Ambion. miRNA purification and labeling and microarray expressionanalyses were performed as described above in Example 2.

The inventors identified 97 miRNAs (38 verified, 59 predicted) withsignificant differential expression between the normal trachea andnormal lymph node samples (|log 2-Diff| trachea vs LN neg≧1 andp-value<0.05) (Table 35). Among the verified miRNAs, hsa-miR-375 and-205 were expressed at levels between 300- and 400-fold higher in thetrachea sample; hsa-miR-429 was expressed at a level between 100- and200-fold higher in trachea; hsa-miR-200a, -200b, and -449 were expressedat levels between 50- and 100-fold higher in trachea; 12 miRNas wereexpressed at levels between 10- and 50-fold higher in trachea; and 12miRNAs were expressed at levels between 5- and 10-fold higher in thetrachea sample than in normal lymph nodes. Among the predicted miRNAs,hsa-asg-13231 st1 and -14153_st2 were expressed at levels over 100-foldhigher in the trachea sample; 4 miRNAs (hsa-asg-4439_st2, -9269_st2,-7637_st2, and cand150 st2) were expressed at levels between 50- and100-fold higher in trachea; 17 miRNAs were expressed at levels between10- and 30-fold higher in trachea; and 15 miRNAs were expressed atlevels between 5- and 10-fold higher in the trachea sample than innormal lymph nodes.

TABLE 35 MicroRNAs Differentially Expressed Between Normal TracheaSamples (TRA) and Cancer-Negative Lymph Node Samples (LNneg) fromPatients. Probe LNneg MicroRNA ID TRA 1 2 3 4 5 6 hsa-miR-375hsa-miR-375_st1 12.78 5.95 4.88 6.24 2.82 2.93 3.63 hsa-miR-205hsa-miR-205_st1 13.08 2.90 5.86 5.64 2.00 3.55 6.06 hsa-miR-429hsa-miR-429_st1 10.31 3.42 1.54 3.66 2.10 2.33 2.65 hsa-asg-13231hsa-asg-13231_st1 10.38 3.91 4.11 4.21 4.44 2.93 3.38 hsa-asg-14153hsa-asg-14153_st2 9.57 3.05 2.23 3.04 2.98 2.33 2.65 hsa-asg-4439hsa-asg-4439_st2 9.86 3.83 4.31 3.21 2.66 1.62 3.63 hsa-miR-200ahsa-miR-200a_st2 12.02 6.26 5.02 6.49 5.71 5.25 4.54 hsa-miR-200bhsa-miR-200b_st1 13.63 7.61 7.75 8.91 6.69 6.79 6.82 hsa-asg-9269hsa-asg-9269_st2 8.90 2.58 2.50 4.68 2.90 2.57 2.65 hsa-asg-7637hsa-asg-7637_st2 9.65 3.64 2.50 4.46 3.44 3.89 3.97 hsa-cand150hsa-cand150_st2 9.46 6.26 3.68 2.40 2.48 3.12 3.43 hsa-miR-449hsa-miR-449_st2 8.38 2.58 1.54 4.54 3.05 3.59 1.67 hsa-miR-187hsa-miR-187_st1 8.73 4.74 2.75 2.74 3.75 3.18 3.32 hsa-miR-34chsa-miR-34c_st2 8.78 1.83 2.50 3.52 3.66 3.59 3.01 hsa-miR-200chsa-miR-200c_st1 14.08 9.59 8.79 10.31 8.17 8.12 8.08 hsa-miR-449bhsa-miR-449b_st1 8.03 1.83 3.24 4.46 2.48 3.55 2.80 hsa-asg-4438hsa-asg-4438_st2 9.40 4.53 4.50 5.04 4.53 4.11 4.49 hsa-asg-924hsa-asg-924_st1 8.16 4.64 4.25 3.38 4.44 2.65 2.73 hsa-asg-12227hsa-asg-12227_st1 6.67 1.63 0.80 2.15 1.18 1.89 1.95 hsa-asg-11715hsa-asg-11715_st2 6.54 1.83 2.32 2.15 1.48 2.07 1.76 hsa-miR-141hsa-miR-141_st1 8.74 5.05 3.78 4.93 4.41 4.36 2.80 hsa-miR-200a*hsa-miR-200a-_st2 7.21 3.05 2.83 2.15 3.89 2.57 1.76 hsa-miR-605hsa-miR-605_st1 6.89 2.58 2.13 3.04 0.73 1.71 2.14 hsa-asg-9830hsa-asg-9830_st2 6.93 3.05 2.50 1.89 2.20 2.16 2.23 hsa-asg-12139hsa-asg-12139_st2 6.51 2.75 2.32 2.63 2.30 1.62 2.14 hsa-asg-2424hsa-asg-2424_st1 5.76 2.22 1.15 1.12 1.79 1.27 1.86 hsa-asg-10247hsa-asg-10247_st1 6.36 2.75 2.13 4.01 1.69 2.16 2.32 hsa-asg-10131hsa-asg-10131_st2 7.04 3.31 3.11 3.52 2.48 2.49 3.20 hsa-miR-203hsa-miR-203_st2 10.56 7.32 5.84 7.22 8.24 6.42 5.36 hsa-asg-1296hsa-asg-1296_st1 6.83 3.05 2.50 4.81 2.39 2.49 2.73 hsa-asg-4219hsa-asg-4219_st1 6.02 3.42 1.74 1.63 1.48 2.24 1.76 hsa-asg-13705hsa-asg-13705_st1 5.83 1.63 0.97 3.78 2.30 2.65 2.32 hsa-asg-3822hsa-asg-3822_st2 6.27 2.03 2.97 3.66 2.00 2.41 2.14 hsa-asg-5119hsa-asg-5119_st1 6.00 2.41 2.67 1.89 1.90 1.98 2.49 hsa-asg-10080hsa-asg-10080_st1 5.81 2.03 1.74 2.40 1.79 2.33 2.40 hsa-miR-383hsa-miR-383_st1 5.93 3.31 2.83 2.40 2.57 3.18 1.30 hsa-miR-495hsa-miR-495_st1 8.64 6.01 4.76 6.97 6.25 6.29 4.38 hsa-asg-4978hsa-asg-4978_st2 5.88 1.63 2.83 2.84 1.69 1.98 1.95 hsa-miR-31hsa-miR-31_st2 12.60 10.40 9.74 8.95 10.1 8.68 8.54 hsa-miR-376ahsa-asg-10049_st1 8.17 5.01 4.85 6.09 5.55 5.31 4.05 hsa-miR-376ahsa-miR-376a_st2 7.46 5.13 4.03 5.49 5.66 4.82 3.97 hsa-miR-376ahsa-asg-14234_st1 6.30 3.31 4.25 3.38 3.98 4.82 3.63 hsa-asg-13254hsa-asg-13254_st1 8.20 5.58 5.32 6.66 5.89 5.87 3.97 hsa-cand317hsa-cand317_st1 5.65 3.42 2.67 1.63 3.12 1.10 1.57 hsa-miR-135bhsa-miR-135b_st2 5.81 1.43 2.67 2.40 2.82 2.33 3.08 hsa-miR-140hsa-asg-14239_st1 9.34 7.74 6.98 6.53 7.45 6.60 5.00 hsa-miR-140hsa-miR-140_st2 9.14 7.72 6.11 6.37 7.21 6.60 5.45 hsa-asg-13908hsa-asg-13908_st2 5.86 2.03 2.50 3.66 2.48 1.44 2.80 hsa-asg-8174hsa-asg-8174_st1 6.39 3.31 4.03 2.84 2.98 2.86 3.20 hsa-miR-539hsa-miR-539_st1 5.65 2.03 3.11 2.63 3.38 2.65 2.94 hsa-cand852hsa-cand852_st2 5.81 3.05 1.94 2.84 2.98 2.24 2.65 hsa-asg-280hsa-asg-280_st1 6.02 3.18 2.67 4.46 2.10 2.49 2.14 hsa-miR-127hsa-miR-127_st1 9.46 6.80 5.52 7.96 7.53 7.66 5.66 hsa-miR-487bhsa-miR-487b_st2 8.51 6.05 5.79 6.97 6.90 6.22 5.34 hsa-miR-154hsa-miR-154_st2 6.39 3.73 2.50 4.30 4.35 4.88 3.08 hsa-asg-7464hsa-asg-7464_st2 6.42 4.59 3.86 4.81 2.48 3.97 2.14 hsa-asg-11160hsa-asg-11160_st2 6.07 2.75 3.11 3.38 3.05 2.86 2.65 hsa-asg-9980hsa-asg-9980_st1 6.74 5.17 4.44 4.01 1.69 3.35 2.80 hsa-miR-96hsa-miR-96_st1 6.09 4.97 3.36 3.04 2.98 4.07 2.80 hsa-miR-410hsa-miR-410_st1 5.33 2.41 2.32 1.37 3.71 2.49 2.80 hsa-asg-11361hsa-asg-11361_st2 6.13 2.75 3.48 3.38 3.25 3.59 3.32 hsa-cand26hsa-cand26_st2 7.67 6.11 5.56 6.04 4.06 3.64 4.70 hsa-asg-1596hsa-asg-1596_st2 6.78 4.41 5.06 4.01 3.19 4.36 4.12 hsa-asg-3251hsa-asg-3251_st1 6.09 4.22 4.11 4.01 2.82 3.50 3.89 hsa-miR-134hsa-miR-134_st2 7.70 5.09 5.73 6.00 5.84 5.67 4.15 hsa-miR-34bhsa-miR-34b_st2 7.92 4.74 5.06 6.41 6.23 5.02 5.18 hsa-miR-329hsa-asg-13589_st2 5.65 1.63 3.11 3.78 3.71 4.38 2.32 hsa-miR-329hsa-miR-329_st1 5.73 3.73 2.97 4.61 3.25 4.54 2.80 hsa-miR-329hsa-asg-13204_st2 5.78 4.07 2.83 4.21 3.38 4.20 3.43 hsa-asg-13135hsa-asg-13135_st1 7.56 5.61 5.61 5.24 4.89 4.76 4.70 hsa-miR-183hsa-miR-183_st1 9.37 7.65 6.71 6.71 7.93 7.54 5.78 hsa-asg-10881hsa-asg-10881_st1 7.57 5.71 4.98 6.06 5.43 4.76 5.40 hsa-asg-13166hsa-asg-13166_st2 6.30 4.47 3.36 4.87 4.94 4.07 4.12 hsa-miR-369-5phsa-miR-369-5p_st2 5.73 3.53 2.67 4.46 4.35 4.33 2.49 hsa-asg-548hsa-asg-548_st1 5.33 3.05 1.94 3.52 3.50 2.79 1.95 hsa-asg-7471hsa-asg-7471_st1 7.89 5.58 5.67 5.68 6.74 5.67 5.25 hsa-miR-485-3phsa-miR-485-3p_st2 5.93 3.64 2.97 4.30 4.41 4.24 3.08 hsa-miR-379hsa-miR-379_st1 8.66 6.51 6.37 7.12 6.96 7.01 6.54 hsa-miR-142-5phsa-miR-142-5p_st2 8.12 6.41 6.67 6.06 6.22 5.77 6.60 hsa-asg-13997hsa-asg-13997_st2 4.42 3.05 3.24 2.15 2.98 2.16 2.32 hsa-miR-182hsa-miR-182_st1 11.13 10.22 9.98 8.86 9.82 9.35 9.50 hsa-miR-132hsa-miR-132_st1 10.15 9.71 8.51 8.34 8.34 8.56 8.02 hsa-asg-3711hsa-asg-3711_st2 6.99 5.69 6.12 5.12 5.52 5.11 5.20 hsa-asg-9411hsa-asg-9411_st1 6.90 5.66 4.61 5.33 5.36 5.05 4.83 hsa-asg-10830hsa-asg-10830_st2 5.70 4.35 3.95 3.66 3.89 3.89 4.43 hsa-asg-14048hsa-asg-14048_st1 6.07 4.41 4.90 3.52 3.60 5.00 3.20 hsa-asg-4513hsa-asg-4513_st2 5.53 3.18 4.44 4.30 2.98 3.40 4.12 hsa-miR-494hsa-miR-494_st1 8.41 6.39 7.25 7.23 6.95 6.60 7.61 hsa-miR-494hsa-miR61_st2 7.88 6.81 6.50 6.86 6.42 6.35 7.05 hsa-asg-3614hsa-asg-3614_st1 6.19 4.79 5.36 3.59 4.38 4.00 5.11 hsa-asg-13298hsa-asg-13298_st1 7.24 5.17 6.20 4.87 5.96 5.40 6.18 hsa-asg-9706hsa-asg-9706_st1 6.78 4.97 4.66 5.90 5.31 5.89 5.90 hsa-asg-8266hsa-asg-8266_st2 5.76 4.15 3.78 4.61 5.22 4.41 4.70 hsa-asg-3046hsa-asg-3046_st2 7.08 5.13 6.50 4.93 5.84 5.09 6.22 hsa-asg-917hsa-asg-917_st2 6.58 5.69 5.25 4.61 4.89 5.89 4.79 hsa-asg-3224hsa-asg-3224_st1 5.56 4.79 3.78 4.46 4.48 4.38 4.32 hsa-miR-510hsa-miR-510_st2 6.07 5.27 5.02 4.75 4.65 4.71 5.04 hsa-asg-10065hsa-asg-10065_st1 5.36 4.47 4.31 3.66 4.70 4.44 3.63 hsa-asg-9575hsa-asg-9575_st1 5.73 4.53 4.38 4.11 4.89 4.33 4.96 hsa-asg-1939hsa-asg-1939_st1 4.60 3.73 3.36 3.52 3.12 3.18 2.94 hsa-asg-5385hsa-asg-5385_st2 6.90 5.79 6.49 5.59 5.50 5.73 6.35 hsa-asg-14233hsa-asg-14233_st1 10.32 9.44 9.21 9.23 10.1 9.41 8.84 hsa-asg-8485hsa-asg-8485_st1 5.83 4.47 4.90 4.99 5.20 4.80 4.19 LNneg MicroRNA 7 8 9M SD % ttest log2Diff Fold hsa-miR-375 1.00 4.61 6.37 4.27 1.82 02.19E−03 −8.51 363 hsa-miR-205 3.38 5.30 7.74 4.71 1.85 33 2.64E−03−8.36 329 hsa-miR-429 2.31 2.41 5.36 2.86 1.14 0 2.60E−04 −7.44 174hsa-asg-13231 1.78 2.05 4.44 3.47 1.01 0 1.95E−04 −6.91 120hsa-asg-14153 1.17 2.74 4.37 2.73 0.85 0 6.18E−05 −6.84 114 hsa-asg-44392.77 3.71 4.04 3.31 0.84 0 7.60E−05 −6.56 94.1 hsa-miR-200a 4.05 4.757.76 5.54 1.15 67 6.81E−04 −6.48 89.4 hsa-miR-200b 6.50 7.64 10.41 7.681.27 100 2.13E−03 −5.96 62.1 hsa-asg-9269 2.62 2.23 3.94 2.96 0.80 01.12E−04 −5.94 61.4 hsa-asg-7637 3.27 3.61 5.24 3.78 0.77 0 8.82E−05−5.87 58.5 hsa-cand150 2.62 3.97 4.44 3.60 1.21 11 1.81E−03 −5.86 58.2hsa-miR-449 0.93 1.66 4.70 2.70 1.37 0 4.29E−03 −5.68 51.3 hsa-miR-1873.03 3.35 3.14 3.33 0.61 0 3.20E−05 −5.40 42.2 hsa-miR-34c 3.48 3.716.83 3.57 1.38 11 7.09E−03 −5.21 37.1 hsa-miR-200c 7.96 8.87 11.51 9.051.21 100 4.35E−03 −5.03 32.7 hsa-miR-449b 2.77 3.17 4.29 3.18 0.84 05.86E−04 −4.85 28.9 hsa-asg-4438 4.18 4.80 5.32 4.61 0.39 0 2.56E−06−4.79 27.7 hsa-asg-924 2.14 2.74 3.50 3.38 0.89 0 9.71E−04 −4.78 27.4hsa-asg-12227 2.77 2.58 2.71 1.96 0.68 0 1.75E−04 −4.71 26.1hsa-asg-11715 0.63 1.46 3.36 1.90 0.74 0 3.47E−04 −4.64 24.9 hsa-miR-1413.83 3.80 4.51 4.16 0.69 0 2.42E−04 −4.58 23.8 hsa-miR-200a* 2.14 3.172.89 2.72 0.64 0 1.67E−04 −4.49 22.5 hsa-miR-605 2.90 3.03 4.44 2.521.03 0 3.86E−03 −4.37 20.7 hsa-asg-9830 3.48 2.23 4.21 2.66 0.76 07.30E−04 −4.27 19.3 hsa-asg-12139 1.60 2.05 3.50 2.32 0.59 0 1.47E−04−4.18 18.2 hsa-asg-2424 2.62 1.66 2.89 1.84 0.63 0 3.83E−04 −3.91 15.1hsa-asg-10247 0.37 3.17 3.94 2.50 1.14 0 1.23E−02 −3.85 14.4hsa-asg-10131 2.62 3.17 5.07 3.22 0.79 0 1.75E−03 −3.82 14.1 hsa-miR-2035.17 6.05 9.34 6.77 1.38 78 3.19E−02 −3.78 13.8 hsa-asg-1296 3.15 3.884.13 3.24 0.85 0 3.94E−03 −3.59 12.1 hsa-asg-4219 2.90 2.74 3.94 2.430.87 0 4.53E−03 −3.59 12.1 hsa-asg-13705 0.37 3.17 3.22 2.27 1.11 01.58E−02 −3.57 11.8 hsa-asg-3822 1.60 2.89 4.76 2.72 0.99 0 9.30E−03−3.55 11.7 hsa-asg-5119 2.90 2.58 3.62 2.49 0.55 0 3.24E−04 −3.51 11.4hsa-asg-10080 2.47 2.23 3.36 2.31 0.48 0 1.19E−04 −3.50 11.3 hsa-miR-3832.62 2.58 1.36 2.46 0.71 0 1.61E−03 −3.47 11.1 hsa-miR-495 5.14 2.414.87 5.23 1.36 56 4.46E−02 −3.41 10.7 hsa-asg-4978 2.77 2.58 4.13 2.490.79 0 3.44E−03 −3.39 10.5 hsa-miR-31 7.71 8.66 10.09 9.21 0.92 1008.02E−03 −3.38 10.4 hsa-miR-376a 3.83 4.12 4.64 4.83 0.75 11 2.93E−03−3.34 10.2 hsa-miR-376a 2.47 3.80 3.73 4.34 1.02 0 1.98E−02 −3.12 8.7hsa-miR-376a 2.90 4.04 1.83 3.57 0.86 0 1.72E−02 −2.73 6.6 hsa-asg-132544.71 2.41 3.36 4.86 1.37 56 5.00E−02 −3.34 10.1 hsa-cand317 3.75 1.852.51 2.40 0.92 0 1.01E−02 −3.24 9.5 hsa-miR-135b 3.27 2.23 2.89 2.570.55 0 5.23E−04 −3.24 9.4 hsa-miR-140 5.69 3.80 5.20 6.11 1.28 674.40E−02 −3.23 9.4 hsa-miR-140 4.76 4.75 5.24 6.02 1.05 44 2.32E−02−3.11 8.6 hsa-asg-13908 2.62 2.41 3.73 2.63 0.72 0 2.84E−03 −3.23 9.4hsa-asg-8174 2.77 2.74 4.13 3.21 0.53 0 4.53E−04 −3.18 9.1 hsa-miR-5391.96 2.58 1.36 2.52 0.63 0 1.55E−03 −3.13 8.7 hsa-cand852 2.90 2.05 3.502.68 0.51 0 4.20E−04 −3.13 8.7 hsa-asg-280 1.78 3.40 4.51 2.97 1.00 02.01E−02 −3.05 8.3 hsa-miR-127 6.57 5.47 5.42 6.51 1.03 56 2.66E−02−2.95 7.7 hsa-miR-487b 4.88 3.71 4.37 5.58 1.11 67 3.69E−02 −2.93 7.6hsa-miR-154 3.15 1.28 3.94 3.47 1.10 0 3.63E−02 −2.92 7.6 hsa-asg-74643.66 2.23 3.84 3.51 0.99 0 2.37E−02 −2.91 7.5 hsa-asg-11160 2.47 3.515.11 3.21 0.79 0 8.90E−03 −2.86 7.2 hsa-asg-9980 3.83 5.05 4.64 3.891.13 11 4.33E−02 −2.85 7.2 hsa-miR-96 3.03 1.85 3.06 3.24 0.87 111.43E−02 −2.85 7.2 hsa-miR-410 3.15 1.85 2.51 2.51 0.68 0 4.44E−03 −2.817.0 hsa-asg-11361 3.83 2.58 4.29 3.39 0.52 0 1.02E−03 −2.74 6.7hsa-cand26 5.27 4.04 5.44 4.98 0.91 22 2.32E−02 −2.69 6.4 hsa-asg-15964.12 4.19 4.37 4.20 0.49 0 1.06E−03 −2.58 6.0 hsa-asg-3251 2.90 3.033.22 3.52 0.55 0 2.17E−03 −2.56 5.9 hsa-miR-134 4.71 4.29 5.00 5.16 0.680 7.87E−03 −2.54 5.8 hsa-miR-34b 4.71 5.09 6.38 5.43 0.71 56 1.01E−02−2.50 5.6 hsa-miR-329 3.66 2.74 3.06 3.16 0.84 0 2.31E−02 −2.49 5.6hsa-miR-329 2.77 3.29 2.71 3.41 0.74 0 1.74E−02 −2.32 5.0 hsa-miR-3293.98 2.58 3.50 3.58 0.59 0 7.78E−03 −2.21 4.6 hsa-asg-13135 5.14 4.445.57 5.11 0.44 22 7.06E−04 −2.45 5.5 hsa-miR-183 7.50 6.30 6.63 6.970.72 89 1.29E−02 −2.40 5.3 hsa-asg-10881 4.18 5.01 5.20 5.19 0.55 223.45E−03 −2.37 5.2 hsa-asg-13166 3.57 2.05 4.04 3.94 0.88 0 3.54E−02−2.36 5.1 hsa-miR-369-5p 2.90 2.58 3.50 3.42 0.81 0 2.64E−02 −2.31 4.9hsa-asg-548 3.38 2.74 4.58 3.05 0.83 0 3.09E−02 −2.28 4.8 hsa-asg-74715.24 4.97 6.07 5.65 0.52 56 3.52E−03 −2.23 4.7 hsa-miR-485-3p 3.66 3.513.84 3.74 0.51 0 3.70E−03 −2.19 4.6 hsa-miR-379 6.33 6.13 6.20 6.58 0.37100 6.65E−04 −2.09 4.2 hsa-miR-142-5p 5.57 5.75 5.51 6.06 0.44 1002.17E−03 −2.05 4.2 hsa-asg-13997 1.25 3.17 2.29 2.51 0.65 0 2.39E−02−1.91 3.8 hsa-miR-182 8.97 8.56 8.11 9.26 0.70 100 3.43E−02 −1.87 3.7hsa-miR-132 8.00 8.42 6.86 8.31 0.74 89 4.50E−02 −1.85 3.6 hsa-asg-37114.52 4.41 4.84 5.17 0.55 0 1.37E−02 −1.82 3.5 hsa-asg-9411 5.03 4.715.24 5.09 0.34 11 1.00E−03 −1.81 3.5 hsa-asg-10830 3.38 3.61 3.84 3.890.34 0 9.37E−04 −1.81 3.5 hsa-asg-14048 4.57 5.13 4.04 4.26 0.70 04.14E−02 −1.80 3.5 hsa-asg-4513 4.12 3.40 3.73 3.74 0.53 0 1.22E−02−1.78 3.4 hsa-miR-494 5.71 5.84 6.58 6.68 0.64 100 3.41E−02 −1.73 3.3hsa-miR-494 5.38 5.77 6.87 6.45 0.55 89 3.91E−02 −1.43 2.7 hsa-asg-36143.83 4.73 4.61 4.49 0.59 0 2.61E−02 −1.70 3.2 hsa-asg-13298 6.00 5.486.20 5.72 0.50 44 2.06E−02 −1.52 2.9 hsa-asg-9706 4.52 5.20 5.07 5.270.53 11 2.68E−02 −1.51 2.8 hsa-asg-8266 4.47 3.40 3.84 4.29 0.55 03.60E−02 −1.47 2.8 hsa-asg-3046 5.64 5.36 5.84 5.62 0.54 78 3.15E−02−1.47 2.8 hsa-asg-917 5.00 4.85 5.20 5.13 0.43 33 1.19E−02 −1.45 2.7hsa-asg-3224 3.48 3.61 4.29 4.18 0.45 0 1.88E−02 −1.38 2.6 hsa-miR-5104.52 4.25 5.07 4.81 0.32 11 5.64E−03 −1.26 2.4 hsa-asg-10065 4.05 3.803.94 4.11 0.39 0 1.53E−02 −1.25 2.4 hsa-asg-9575 4.71 4.12 4.37 4.490.31 0 5.32E−03 −1.24 2.4 hsa-asg-1939 3.38 3.51 3.73 3.39 0.27 112.73E−03 −1.22 2.3 hsa-asg-5385 5.20 5.66 5.86 5.80 0.40 33 3.08E−02−1.11 2.2 hsa-asg-14233 9.03 8.85 8.87 9.23 0.41 100 3.75E−02 −1.09 2.1hsa-asg-8485 4.57 5.13 4.87 4.79 0.33 0 1.62E−02 −1.04 2.1 SD, standarddeviation; %, percentage of samples expressing miRNA above backgroundlevels; Log2Diff, difference in VSN-transformed expression bewteen TRAand LNneg samples.

Example 37 miRNAs Differentally Expressed Between Normal Small IntestineTissue and Normal Lymph Nodes

To identify miRNAs that may be useful markers for metastasis ofcancerous small intestine cells to lymph nodes, the inventors identifiedmiRNAs that are expressed at significantly higher levels in normal smallintestine tissue than in cancer-negative lymph nodes.

Total RNA, purified from one normal human small intestine sample(FirstChoice® Human Small Intestine Total RNA, cat. no. AM7984; Ambion,Austin, Tex., USA) and from nine normal human lymph node samples(FirstChoice® Human Lymph Node Total RNA, cat. no. AM7894; Ambion,Austin, Tex., USA) was purchased from Ambion. miRNA purification andlabeling and microarray expression analyses were performed as describedabove in Example 2.

The inventors identified 413 miRNAs (28 verified, 385 predicted) withsignificant differential expression between the normal small intestineand normal lymph node samples (|log 2-Diff| small intestine vs LN neg≧1and p-value<0.05) (Table 36) Among the verified miRNAs, hsa-miR-429 and-375 were expressed at levels between 85- and 100-fold higher in thesmall intestine sample; 4 miRNAs (hsa-miR-215, -200a, -187, and -200b)were expressed at levels between 25- and 50-fold higher in smallintestine; 5 miRNAs (hsa-miR-141, -200c, -605, -194, and -802) wereexpressed at levels between 10- and 20-fold higher in small intestine;and 5 miRNAs were expressed at levels between 5- and 10-fold higher inthe small intestine sample than in normal lymph nodes. Among thepredicted miRNAs, 6 miRNA (hsa-asg-9939_st2, -7846_st1, -6880 st1,-14182_st1, -6838 st1, and -924 st1) were expressed at levels between25- and 40-fold higher in the small intestine sample; 13 miRNAs wereexpressed at levels between 10- and 20-fold higher in small intestine;and 56 miRNAs were expressed at levels between 5- and 10-fold higher inthe small intestine sample than in normal lymph nodes.

TABLE 36 MicroRNAs Differentially Expressed Between Normal SmallIntestine Samples (SI) and Cancer-Negative Lymph Node Samples (LNneg)from Patients. Probe LNneg MicroRNA ID SI 1 2 3 5 6 7 hsa-miR-429hsa-miR-429_st1 9.49 3.42 1.54 3.66 2.10 2.33 2.65 2.31 hsa-miR-375hsa-miR-375_st1 10.70 5.95 4.88 6.24 2.82 2.93 3.63 1.00 hsa-miR-215hsa-miR-215_st1 11.33 6.95 5.77 5.71 6.66 5.51 4.79 5.49 hsa-asg-9939hsa-asg-9939_st2 7.85 2.22 2.32 2.84 2.30 2.24 3.72 1.09 hsa-asg-7846hsa-asg-7846_st1 7.98 4.07 2.41 1.76 2.57 2.93 1.39 2.90 hsa-asg-6880hsa-asg-6880_st1 11.08 5.50 6.43 5.64 5.81 5.80 5.77 5.45 hsa-miR-200ahsa-miR-200a_st2 10.52 6.26 5.02 6.49 5.71 5.25 4.54 4.05 hsa-miR-187hsa-miR-187_st1 8.30 4.74 2.75 2.74 3.75 3.18 3.32 3.03 hsa-asg-14182hsa-asg-14182_st1 6.78 2.41 0.97 1.89 1.79 2.57 1.76 2.77 hsa-miR-200bhsa-miR-200b_st1 12.57 7.61 7.75 8.91 6.69 6.79 6.82 6.50 hsa-asg-6838hsa-asg-6838_st1 11.03 5.81 6.21 5.69 6.12 6.49 6.23 5.69 hsa-asg-924hsa-asg-924_st1 8.03 4.64 4.25 3.38 4.44 2.65 2.73 2.14 hsa-miR-141hsa-miR-141_st1 8.47 5.05 3.78 4.93 4.41 4.36 2.80 3.83 hsa-asg-13498hsa-asg-13498_st1 7.65 3.91 4.58 1.76 1.28 5.42 5.30 1.25 hsa-asg-8330hsa-asg-8330_st2 8.93 3.18 5.50 4.46 4.78 4.88 5.49 3.83 hsa-miR206hsa-miR206_st1 8.57 3.42 5.53 2.84 4.48 3.97 5.67 3.83 hsa-miR-200chsa-miR-200c_st1 13.09 9.59 8.79 10.31 8.17 8.12 8.08 7.96 hsa-asg-7637hsa-asg-7637_st2 7.73 3.64 2.50 4.46 3.44 3.89 3.97 3.27 hsa-asg-12552hsa-asg-12552_st1 8.11 4.07 3.78 3.52 4.44 3.40 4.15 3.83 hsa-cand317hsa-cand317_st1 6.23 3.42 2.67 1.63 3.12 1.10 1.57 3.75 hsa-asg-11715hsa-asg-11715_st2 5.50 1.83 2.32 2.15 1.48 2.07 1.76 0.63 hsa-asg-6377hsa-asg-6377_st2 6.24 2.22 2.83 3.66 1.90 1.71 3.43 2.77 hsa-asg-3595hsa-asg-3595_st1 6.82 2.58 3.78 2.15 2.66 1.71 4.12 3.27 hsa-miR-605hsa-miR-605_st1 6.07 2.58 2.13 3.04 0.73 1.71 2.14 2.90 hsa-asg-9262hsa-asg-9262_st1 7.94 4.59 4.28 3.96 5.74 4.71 4.52 3.75 hsa-asg-11612hsa-asg-11612_st1 8.52 5.17 4.96 4.71 5.67 4.54 4.65 4.62 hsa-miR-194hsa-miR-194_st2 12.34 9.82 9.32 8.92 9.34 8.92 8.29 8.54 hsa-miR-802hsa-miR140_st1 5.08 1.24 2.32 1.37 1.48 1.80 1.76 0.78 hsa-miR-802hsa-asg-353_st1 5.15 2.41 2.97 2.63 2.48 2.49 0.87 0.63 hsa-asg-6027hsa-asg-6027_st2 12.10 7.74 8.47 8.57 8.01 9.01 8.09 8.52 hsa-asg-4691hsa-asg-4691_st1 9.24 5.81 6.57 5.69 5.43 5.74 6.28 5.03 hsa-miR-31hsa-miR-31_st2 12.52 10.40 9.74 8.95 10.15 8.68 8.54 7.71 hsa-asg-3667hsa-asg-3667_st2 5.97 2.03 2.83 2.84 2.00 2.93 2.57 2.31 hsa-asg-12983hsa-asg-12983_st2 5.82 1.83 3.24 1.89 2.20 2.93 3.68 0.63 hsa-asg-3046hsa-asg-3046_st2 8.87 5.13 6.50 4.93 5.84 5.09 6.22 5.64 hsa-asg-9082hsa-asg-9082_st1 6.21 2.75 4.47 1.24 2.90 3.06 3.63 2.47 hsa-cand601hsa-cand601_st2 6.20 3.31 4.38 1.89 1.48 2.86 3.43 3.15 hsa-asg-13298hsa-asg-13298_st1 8.91 5.17 6.20 4.87 5.96 5.40 6.18 6.00 hsa-miR-200a*hsa-miR-200a-_st2 5.88 3.05 2.83 2.15 3.89 2.57 1.76 2.14 hsa-asg-2154hsa-asg-2154_st1 5.38 1.43 2.97 1.63 1.59 2.49 2.87 0.63 hsa-asg-12799hsa-asg-12799_st1 7.19 3.53 5.30 3.30 2.90 4.20 5.40 3.91 hsa-asg-13705hsa-asg-13705_st1 5.24 1.63 0.97 3.78 2.30 2.65 2.32 0.37 hsa-asg-11160hsa-asg-11160_st2 6.16 2.75 3.11 3.38 3.05 2.86 2.65 2.47 hsa-asg-12058hsa-asg-12058_st1 5.27 1.83 1.74 2.84 2.00 2.99 3.43 1.09 hsa-asg-10481hsa-asg-10481_st1 6.16 2.41 3.95 3.66 2.90 2.24 3.32 3.98 hsa-miR-192hsa-miR-192_st2 12.49 10.83 9.82 10.13 10.00 9.55 8.66 9.31hsa-asg-11410 hsa-asg-11410_st1 5.37 2.90 3.68 2.84 3.19 2.24 1.21 1.60hsa-asg-11320 hsa-asg-11320_st2 7.03 4.88 4.44 4.11 4.10 4.36 4.05 3.83hsa-asg-10131 hsa-asg-10131_st2 6.04 3.31 3.11 3.52 2.48 2.49 3.20 2.62hsa-asg-12139 hsa-asg-12139_st2 5.12 2.75 2.32 2.63 2.30 1.62 2.14 1.60hsa-asg-11926 hsa-asg-11926_st1 5.34 1.83 3.24 1.63 1.90 2.41 3.08 1.42hsa-asg-13030 hsa-asg-13030_st1 7.06 4.07 4.94 3.78 4.28 4.41 4.54 3.48hsa-asg-5986 hsa-asg-5986_st1 9.89 6.99 7.15 6.21 7.52 6.14 6.77 8.00hsa-cand899 hsa-cand899_st2 6.75 2.75 4.74 3.30 3.98 4.17 4.77 3.38hsa-asg-5119 hsa-asg-5119_st1 5.19 2.41 2.67 1.89 1.90 1.98 2.49 2.90hsa-asg-7095 hsa-asg-7095_st1 8.17 4.88 5.49 5.17 5.15 5.03 5.39 5.84hsa-asg-4219 hsa-asg-4219_st1 5.10 3.42 1.74 1.63 1.48 2.24 1.76 2.90hsa-asg-4066 hsa-asg-4066_st2 6.29 2.75 5.02 2.15 3.55 3.35 2.80 4.05hsa-miR-9 hsa-cand90_st2 5.44 3.53 3.11 2.63 2.98 2.79 2.14 2.62hsa-miR-9 hsa-miR-9_st2 5.89 3.42 3.48 2.84 3.25 3.59 2.14 3.48hsa-asg-8239 hsa-asg-8239_st1 5.86 3.18 3.95 1.89 2.82 3.73 3.20 2.77hsa-asg-10411 hsa-asg-10411_st1 5.84 2.41 2.67 4.21 3.55 2.57 4.49 3.15hsa-asg-4015 hsa-asg-4015_st2 7.83 5.37 6.12 5.07 5.98 4.76 5.64 3.03hsa-asg-3036 hsa-asg-3036_st1 6.27 3.18 3.73 5.12 3.05 2.65 2.49 3.27hsa-asg-1260 hsa-asg-1260_st1 5.20 2.41 2.83 2.40 3.19 2.57 3.08 2.90hsa-asg-7262 hsa-asg-7262_st1 8.10 4.59 6.34 5.09 4.85 5.79 5.79 5.17hsa-cand830 hsa-cand830_st2 5.41 1.05 2.83 2.15 3.44 3.64 2.80 3.03hsa-asg-9752 hsa-asg-9752_st2 7.19 4.35 4.25 4.54 4.32 4.52 3.97 4.84hsa-asg-7014 hsa-asg-7014_st2 6.34 2.58 4.83 3.72 3.71 3.81 5.26 2.14hsa-asg-2551 hsa-asg-2551_st1 7.60 4.15 6.06 4.16 4.75 3.55 5.91 5.10hsa-asg-4870 hsa-asg-4870_st1 6.44 3.05 4.21 4.42 3.80 4.17 4.67 1.78hsa-asg-4685 hsa-asg-4685_st1 6.35 3.31 4.50 3.52 3.25 4.36 3.32 3.03hsa-asg-6071 hsa-asg-6071_st2 6.08 2.41 3.86 2.63 4.25 3.45 3.81 3.27hsa-asg-3521 hsa-asg-3521_st2 4.92 1.83 3.58 3.38 2.00 1.98 1.12 2.31hsa-asg-12755 hsa-asg-12755_st2 6.62 3.64 5.81 3.66 3.80 3.18 4.38 3.48hsa-asg-12625 hsa-asg-12625_st1 6.26 4.22 5.33 3.59 3.44 3.18 4.05 3.57hsa-asg-2738 hsa-asg-2738_st2 7.70 4.35 6.02 4.42 4.51 5.16 5.94 5.33hsa-asg-12941 hsa-asg-12941_st1 10.64 8.98 8.54 7.03 8.48 8.59 7.83 8.03hsa-miR-381 hsa-miR-381_st2 5.50 2.75 2.32 4.21 3.38 4.17 2.94 3.48hsa-asg-12590 hsa-asg-12590_st1 5.67 2.22 4.38 2.40 2.30 3.93 3.63 3.27hsa-asg-6773 hsa-asg-6773_st1 5.05 2.41 3.24 2.63 1.38 3.29 3.48 2.62hsa-asg-13797 hsa-asg-13797_st2 5.78 2.41 3.48 2.84 2.98 3.40 3.63 2.90hsa-asg-8922 hsa-asg-8922_st2 9.65 6.85 8.18 6.30 6.38 7.72 8.16 6.42hsa-asg-1689 hsa-asg-1689_st2 7.80 5.58 5.96 5.21 5.13 5.54 5.43 4.76hsa-asg-11219 hsa-asg-11219_st1 5.88 3.31 5.10 3.52 2.82 2.24 3.72 3.38hsa-asg-13450 hsa-asg-13450_st1 5.20 2.90 3.68 2.63 2.20 2.99 1.21 3.03hsa-asg-12365 hsa-asg-12365_st1 7.47 4.00 6.58 4.21 4.48 4.71 5.67 4.96hsa-asg-933 hsa-asg-933_st2 9.58 7.05 7.45 6.69 7.03 7.41 7.31 6.23hsa-asg-8174 hsa-asg-8174_st1 5.55 3.31 4.03 2.84 2.98 2.86 3.20 2.77hsa-asg-9574 hsa-asg-9574_st2 8.19 6.61 6.74 4.46 5.50 5.60 6.11 5.03hsa-asg-8397 hsa-asg-8397_st2 5.96 3.18 5.02 3.38 2.90 3.50 4.22 2.14hsa-asg-9319 hsa-asg-9319_st2 6.85 3.91 5.06 4.38 3.38 5.07 4.32 3.83hsa-asg-4080 hsa-asg-4080_st2 5.15 3.31 3.11 1.12 2.82 2.49 2.65 3.66hsa-asg-3900 hsa-asg-3900_st1 5.14 3.18 3.24 3.21 3.32 2.57 2.80 3.27hsa-asg-11056 hsa-asg-11056_st1 5.65 2.41 4.11 2.84 3.25 2.99 3.89 3.15hsa-asg-4915 hsa-asg-4915_st1 5.26 2.41 3.78 2.15 2.57 3.59 2.87 2.77hsa-asg-6500 hsa-asg-6500_st1 7.05 4.74 4.81 4.68 4.02 5.11 5.31 3.66hsa-asg-3716 hsa-asg-3716_st1 7.38 5.13 5.87 3.45 4.89 5.72 4.90 4.67hsa-asg-1351 hsa-asg-1351_st1 6.28 4.00 5.21 3.38 2.98 3.73 4.12 3.27hsa-asg-7385 hsa-asg-7385_st1 7.60 4.84 5.65 4.84 5.09 5.19 5.57 5.62hsa-asg-9879 hsa-asg-9879_st1 5.44 2.22 4.25 2.63 3.32 3.93 3.14 2.77hsa-asg-6381 hsa-asg-6381_st1 6.10 4.22 4.85 2.40 3.98 3.64 4.25 3.27hsa-miR-124a hsa-cand79_st1 5.77 3.53 3.78 3.78 4.44 3.64 2.14 2.31hsa-asg-8986 hsa-asg-8986_st1 7.22 4.53 5.96 4.25 4.98 3.97 5.23 4.62hsa-asg-10457 hsa-asg-10457_st1 5.83 3.05 3.78 2.15 3.66 3.89 4.65 2.62hsa-asg-11656 hsa-asg-11656_st1 6.86 3.83 5.00 4.42 5.05 4.20 4.88 4.67hsa-asg-9573 hsa-asg-9573_st2 5.22 2.90 3.58 2.63 2.48 3.40 2.14 3.15hsa-asg-8602 hsa-asg-8602_st2 7.76 4.79 6.41 5.31 5.43 5.24 5.91 4.80hsa-asg-3061 hsa-asg-3061_st2 7.66 5.86 6.26 4.75 5.31 5.14 5.34 4.84hsa-asg-6135 hsa-asg-6135_st2 6.32 3.73 4.50 4.21 3.55 4.96 4.49 4.18hsa-asg-8972 hsa-asg-8972_st2 5.23 1.83 3.48 1.89 2.20 3.24 3.53 3.91hsa-asg-8703 hsa-asg-8703_st1 5.55 2.58 3.11 1.89 3.85 3.18 3.58 3.66hsa-asg-8522 hsa-asg-8522_st2 6.52 4.74 4.55 4.11 4.67 4.11 3.72 3.57hsa-asg-6116 hsa-asg-6116_st2 4.63 2.41 2.13 1.89 1.90 2.79 3.20 3.15hsa-asg-9781 hsa-asg-9781_st1 7.23 4.00 5.93 4.75 4.32 4.57 5.62 4.71hsa-asg-1727 hsa-asg-1727_st2 7.14 5.13 6.02 4.25 4.78 4.86 5.46 3.48hsa-asg-2894 hsa-asg-2894_st2 5.37 3.18 3.86 2.84 1.79 2.99 2.65 3.83hsa-asg-12922 hsa-asg-12922_st1 6.59 4.47 4.55 3.21 4.56 4.52 4.88 3.98hsa-asg-1715 hsa-asg-1715_st2 5.40 3.53 3.78 3.21 2.30 2.57 3.20 4.18hsa-asg-10580 hsa-asg-10580_st1 10.34 7.42 8.56 7.69 8.41 7.64 8.71 8.21hsa-asg-7183 hsa-asg-7183_st1 5.86 3.42 4.71 3.38 3.32 3.45 3.32 3.83hsa-asg-11797 hsa-asg-11797_st1 7.60 5.61 6.64 3.96 5.20 5.25 6.25 4.71hsa-asg-13091 hsa-asg-13091_st2 8.38 4.79 7.10 5.93 6.02 5.55 7.27 6.02hsa-asg-12684 hsa-asg-12684_st2 10.09 8.07 8.42 7.61 7.79 7.86 8.14 7.41hsa-asg-3841 hsa-asg-3841_st2 8.61 6.36 5.97 5.64 6.58 6.98 6.76 5.94hsa-asg-7228 hsa-asg-7228_st1 7.33 5.79 6.36 4.25 4.59 5.39 5.95 4.71hsa-asg-8643 hsa-asg-8643_st2 6.98 3.64 5.44 5.28 4.78 4.30 5.43 5.00hsa-asg-1296 hsa-asg-1296_st1 5.34 3.05 2.50 4.81 2.39 2.49 2.73 3.15hsa-asg-5662 hsa-asg-5662_st2 6.48 4.53 5.60 3.96 4.96 3.85 4.79 3.03hsa-asg-6614 hsa-asg-6614_st1 6.40 4.07 5.21 2.63 3.75 5.00 4.49 3.98hsa-asg-12119 hsa-asg-12119_st2 6.38 4.15 4.81 4.11 4.80 3.68 4.38 3.57hsa-asg-9053 hsa-asg-9053_st1 7.93 5.86 6.50 4.78 6.21 6.07 6.86 4.88hsa-asg-9270 hsa-asg-9270_st1 4.87 2.41 3.58 2.15 3.44 2.65 3.68 1.42hsa-cand894 hsa-cand894_st1 5.50 1.83 4.11 3.04 4.02 3.24 3.81 3.27hsa-asg-1513 hsa-asg-1513_st1 6.80 3.18 5.75 4.25 5.26 4.84 4.98 4.76hsa-asg-191 hsa-asg-191_st1 5.77 3.31 3.86 2.84 3.55 3.81 4.05 3.98hsa-asg-3815 hsa-asg-3815_st1 6.56 3.18 5.32 4.75 4.02 4.69 4.52 3.48hsa-asg-10181 hsa-asg-10181_st1 7.25 4.64 6.19 5.37 4.38 5.07 5.70 4.52hsa-asg-12697 hsa-asg-12697_st1 5.96 3.31 4.31 4.01 3.60 3.85 4.38 3.75hsa-asg-6269 hsa-asg-6269_st2 7.45 5.47 6.21 4.25 4.51 5.02 5.49 4.88hsa-asg-10114 hsa-asg-10114_st1 6.16 4.41 4.90 4.54 3.89 3.81 5.31 3.75hsa-asg-10399 hsa-asg-10399_st1 5.98 3.73 4.76 4.11 3.25 3.73 4.12 3.75hsa-asg-13266 hsa-asg-13266_st1 5.17 3.31 4.11 3.04 2.48 3.93 2.80 2.47hsa-asg-6000 hsa-asg-6000_st1 6.94 5.09 5.65 4.25 4.67 4.86 5.07 3.98hsa-asg-7166 hsa-asg-7166_st2 5.32 2.58 3.86 3.21 2.66 3.12 3.20 3.48hsa-asg-466 hsa-asg-466_st1 6.61 4.07 4.63 2.74 4.96 5.22 4.43 4.84hsa-asg-6934 hsa-asg-6934_st2 5.16 2.75 4.50 2.40 2.57 2.72 3.97 2.77hsa-asg-265 hsa-asg-265_st2 7.98 6.45 7.13 5.14 5.70 5.87 7.03 5.27hsa-asg-13334 hsa-asg-13334_st2 5.00 3.05 3.36 2.15 3.25 3.45 2.80 3.27hsa-asg-5712 hsa-asg-5712_st2 8.23 5.20 6.25 6.05 6.23 5.84 6.36 6.09hsa-asg-4829 hsa-asg-4829_st1 11.16 8.07 9.70 8.28 8.56 9.57 9.40 8.62hsa-asg-1596 hsa-asg-1596_st2 6.21 4.41 5.06 4.01 3.19 4.36 4.12 4.12hsa-asg-7278 hsa-asg-7278_st1 7.02 5.20 5.73 4.34 4.92 5.28 5.54 3.98hsa-miR-202 hsa-miR-202_st1 6.84 4.69 5.02 4.38 4.96 5.02 5.58 3.66hsa-asg-244 hsa-asg-244_st1 6.62 3.31 5.23 4.65 4.44 4.14 5.21 4.52hsa-asg-3614 hsa-asg-3614_st1 6.47 4.79 5.36 3.59 4.38 4.00 5.11 3.83hsa-asg-3289 hsa-asg-3289_st1 5.48 3.64 4.25 3.21 3.89 3.06 3.81 2.47hsa-asg-11361 hsa-asg-11361_st2 5.36 2.75 3.48 3.38 3.25 3.59 3.32 3.83hsa-asg-8953 hsa-asg-8953_st1 9.78 6.78 8.45 7.10 7.73 7.26 8.40 7.21hsa-asg-572 hsa-asg-572_st2 6.42 4.69 5.27 3.84 3.66 3.93 5.21 3.48hsa-asg-11796 hsa-asg-11796_st2 7.40 4.64 5.74 4.61 5.38 5.72 5.60 5.52hsa-cand630 hsa-cand630_st2 7.05 5.17 6.10 5.26 4.53 4.96 5.64 3.75hsa-asg-9085 hsa-asg-9085_st1 7.47 5.44 6.38 4.90 5.18 5.47 6.50 3.98hsa-asg-3150 hsa-asg-3150_st1 8.11 6.39 7.40 5.51 6.17 5.96 6.77 5.67hsa-asg-6734 hsa-asg-6734_st2 4.81 2.41 4.18 2.84 2.74 3.06 2.73 1.96hsa-asg-1010 hsa-asg-10104_st2 6.67 3.53 5.74 4.87 4.44 5.11 5.37 3.91hsa-asg-7067 hsa-asg-7067_st1 6.44 4.35 5.41 3.38 4.35 4.17 5.07 4.12hsa-asg-11875 hsa-asg-11875_st1 5.82 4.53 4.50 3.38 2.82 2.99 4.05 3.48hsa-asg-9320 hsa-asg-9320_st2 6.74 4.41 5.88 4.38 4.28 3.97 5.21 4.88hsa-asg-9873 hsa-asg-9873_st1 4.90 2.58 4.11 1.63 2.57 2.65 3.01 3.15hsa-asg-5115 hsa-asg-5115_st1 9.09 6.97 8.00 6.75 7.15 6.96 7.34 6.17hsa-asg-12556 hsa-asg-12556_st1 9.59 8.06 8.67 6.55 7.78 8.32 8.39 6.96hsa-asg-807 hsa-asg-807_st2 6.79 4.15 5.41 4.54 4.35 4.71 5.52 4.57hsa-asg-9025 hsa-asg-9025_st2 7.15 4.35 5.41 4.75 5.00 5.27 5.40 4.62hsa-asg-12652 hsa-asg-12652_st1 4.72 3.31 2.67 3.66 2.30 2.65 3.43 1.78hsa-asg-9547 hsa-asg-9547_st2 5.89 3.53 4.90 3.78 3.32 3.40 3.85 3.38hsa-asg-5385 hsa-asg-5385_st2 7.71 5.79 6.49 5.59 5.50 5.73 6.35 5.20hsa-asg-13661 hsa-asg-13661_st1 6.94 5.64 5.46 3.30 4.92 5.46 5.18 4.76hsa-asg-7178 hsa-asg-7178_st1 6.97 4.29 6.06 4.16 4.48 5.00 5.69 4.42hsa-cand173 hsa-cand173_st1 7.98 6.74 7.22 5.91 5.65 6.21 6.24 5.03hsa-asg-1981 hsa-asg-1981_st1 5.50 3.64 4.76 3.52 2.82 3.35 3.53 2.90hsa-asg-477 hsa-asg-477_st1 7.08 4.97 5.88 4.11 4.73 4.76 5.75 4.67hsa-asg-11742 hsa-asg-11742_st2 5.80 3.42 4.61 3.04 3.32 4.57 4.65 2.77hsa-asg-7776 hsa-asg-7776_st2 6.48 3.73 5.30 4.34 3.89 4.24 5.50 5.07hsa-asg-7215 hsa-asg-7215_st2 7.21 5.31 6.09 4.81 5.78 5.12 5.71 4.71hsa-asg-4814 hsa-asg-4814_st1 8.13 6.51 7.06 5.35 6.03 6.30 6.85 5.41hsa-asg-9163 hsa-asg-9163_st2 5.74 4.07 4.76 2.84 3.80 3.24 4.43 2.90hsa-asg-9453 hsa-asg-9453_st2 8.82 7.57 8.10 6.21 6.88 7.39 7.53 6.23hsa-asg-8404 hsa-asg-8404_st2 5.49 2.22 4.25 3.04 3.55 4.17 3.63 3.57hsa-asg-8926 hsa-asg-8926_st1 6.88 4.59 5.43 4.25 4.75 5.03 5.56 4.62hsa-asg-10962 hsa-asg-10962_st1 5.23 2.58 2.67 4.21 3.05 2.99 4.05 3.03hsa-asg-225 hsa-asg-225_st1 7.45 5.64 6.52 5.12 5.75 5.48 5.79 4.84hsa-asg-8512 hsa-asg-8512_st2 5.89 4.41 4.44 3.21 4.21 4.04 3.63 3.48hsa-asg-6941 hsa-asg-6941_st2 6.31 3.42 4.25 4.11 4.02 4.71 5.31 4.18hsa-asg-956 hsa-asg-956_st2 4.81 3.53 3.58 2.84 2.48 2.33 3.32 2.47hsa-asg-7344 hsa-asg-7344_st2 6.32 3.91 5.25 4.21 4.02 4.41 4.12 4.52hsa-cand624 hsa-cand624_st1 8.69 6.15 7.84 5.97 6.52 6.47 7.40 6.19hsa-asg-12925 hsa-asg-12925_st1 9.30 8.27 8.12 6.40 7.75 7.69 7.64 6.72hsa-asg-12989 hsa-asg-12989_st2 5.51 3.64 4.31 2.63 3.32 3.40 3.68 4.12hsa-asg-6618 hsa-asg-6618_st2 7.22 5.47 5.94 4.50 5.31 5.71 6.06 4.92hsa-asg-3296 hsa-asg-3296_st1 6.02 3.73 4.81 3.21 3.25 4.71 4.83 3.91hsa-asg-3003 hsa-asg-3003_st1 5.84 3.73 4.90 4.01 3.05 4.24 4.60 2.62hsa-asg-3155 hsa-asg-3155_st1 7.20 5.64 5.47 5.37 4.89 5.61 4.92 5.27hsa-miR-7 hsa-miR-7_st1 10.44 9.45 8.18 9.71 8.61 8.16 7.65 8.64hsa-asg-6792 hsa-asg-6792_st1 7.90 5.86 6.91 5.26 5.93 6.10 6.84 5.10hsa-asg-12395 hsa-asg-12395_st2 7.48 4.93 5.80 4.78 5.96 6.82 6.08 5.20hsa-asg-2045 hsa-asg-2045_st1 8.23 5.69 7.16 5.74 6.46 5.94 6.89 5.69hsa-asg-3745 hsa-asg-3745_st1 8.10 6.03 7.05 5.41 5.93 6.90 6.19 5.53hsa-miR189 hsa-miR189_st2 6.40 4.00 5.60 3.84 4.38 4.64 5.40 4.36hsa-asg-4767 hsa-asg-4767_st1 6.46 4.35 5.57 4.84 3.89 4.47 4.15 4.12hsa-asg-9849 hsa-asg-9849_st2 8.49 6.37 7.98 5.47 6.39 6.76 7.48 6.11hsa-asg-394 hsa-asg-394_st1 5.42 3.42 3.95 3.66 2.48 3.59 3.89 3.57hsa-asg-4061 hsa-asg-4061_st1 8.69 5.79 7.95 6.30 6.52 6.79 7.36 6.47hsa-asg-8931 hsa-asg-8931_st1 6.27 4.59 4.85 3.90 4.32 4.04 4.25 4.76hsa-asg-4055 hsa-asg-4055_st1 5.28 3.05 2.50 3.52 3.38 3.97 3.72 3.57hsa-asg-6866 hsa-asg-6866_st2 5.14 1.83 3.48 3.04 3.44 3.45 3.63 3.15hsa-asg-10802 hsa-asg-10802_st2 6.13 4.22 5.10 3.52 3.89 4.30 4.88 3.66hsa-asg-13197 hsa-asg-13197_st1 4.66 2.41 2.67 3.38 3.25 2.16 2.94 2.47hsa-asg-4964 hsa-asg-4964_st2 6.63 4.79 5.02 3.21 4.78 5.05 4.98 4.67hsa-asg-242 hsa-asg-242_st2 6.43 4.64 4.90 5.19 4.82 4.41 4.32 3.48hsa-asg-4693 hsa-asg-4693_st1 6.93 5.05 5.74 4.21 5.38 5.00 5.21 4.52hsa-asg-1127 hsa-asg-1127_st2 5.32 3.64 4.03 3.52 2.98 3.64 4.19 3.75hsa-asg-1172 hsa-asg-1172_st2 6.42 4.97 5.32 4.01 4.32 5.50 5.54 4.05hsa-asg-12214 hsa-asg-12214_st1 6.53 4.22 5.65 4.50 4.28 4.24 4.94 4.47hsa-asg-1241 hsa-asg-1241_st2 8.85 6.75 7.68 5.81 6.87 7.23 7.62 6.45hsa-asg-10832 hsa-asg-10832_st1 6.06 4.35 4.94 3.66 4.28 3.64 4.43 3.57hsa-cand668 hsa-cand668_st1 9.39 6.64 7.73 6.89 7.67 7.60 7.76 7.33hsa-asg-7900 hsa-asg-7900_st1 6.87 4.41 6.30 4.50 5.03 4.47 5.66 4.36hsa-miR-518c* hsa-miR-518c-_st2 5.78 3.83 5.14 4.38 3.12 3.35 4.38 3.66hsa-asg-1576 hsa-asg-1576_st1 9.26 7.85 8.09 6.65 7.36 8.04 7.46 7.09hsa-asg-3109 hsa-asg-3109_st2 5.60 4.53 4.18 2.84 3.89 4.90 4.25 2.90hsa-asg-3771 hsa-asg-3771_st1 6.05 3.42 4.38 4.11 4.62 5.00 4.74 3.66hsa-asg-4440 hsa-asg-4440_st1 7.20 5.93 5.71 5.51 5.83 6.14 5.49 4.88hsa-asg-7671 hsa-asg-7671_st1 7.99 6.24 7.39 5.76 6.13 6.82 6.90 5.60hsa-asg-11795 hsa-asg-11795_st2 7.17 5.09 6.45 5.45 4.92 5.99 5.72 4.76hsa-asg-8731 hsa-asg-8731_st2 6.18 3.91 4.85 4.30 4.02 4.80 4.25 3.83hsa-asg-10510 hsa-asg-10510_st2 5.59 3.42 4.18 3.90 3.66 2.72 3.53 4.31hsa-asg-3744 hsa-asg-3744_st1 6.59 4.74 5.69 4.38 5.13 4.90 5.48 3.38hsa-asg-1716 hsa-asg-1716_st1 6.54 4.59 5.36 4.50 4.38 4.38 5.11 4.18hsa-asg-4703 hsa-asg-4703_st2 6.95 5.34 6.13 4.61 4.87 5.70 5.84 4.76hsa-asg-13135 hsa-asg-13135_st1 6.81 5.61 5.61 5.24 4.89 4.76 4.70 5.14hsa-asg-5326 hsa-asg-5326_st2 7.99 6.95 7.13 5.74 6.26 6.65 6.47 5.52hsa-asg-11898 hsa-asg-11898_st2 6.42 4.53 5.50 4.75 3.98 4.66 4.88 3.91hsa-miR-565 hsa-miR-565_st1 8.93 7.20 7.07 7.80 6.92 5.73 7.61 7.76hsa-asg-7224 hsa-asg-7224_st2 8.55 6.70 7.80 5.73 7.16 7.20 7.60 6.09hsa-asg-3970 hsa-asg-3970_st2 4.49 2.75 3.36 1.89 2.30 2.16 3.43 2.31hsa-asg-9334 hsa-asg-9334_st2 8.40 6.32 7.60 6.15 6.41 6.92 7.31 6.22hsa-asg-92 hsa-asg-92_st1 6.17 4.15 5.53 4.01 4.14 4.24 5.15 3.83hsa-asg-7174 hsa-asg-7174_st2 7.74 5.20 6.63 5.79 5.33 5.76 7.07 5.78hsa-asg-7133 hsa-asg-7133_st1 7.36 4.88 6.33 5.62 5.00 5.66 6.13 4.96hsa-asg-7023 hsa-asg-7023_st2 6.02 4.15 4.55 4.21 4.02 4.57 4.38 4.18hsa-asg-11096 hsa-asg-11096_st1 8.25 5.74 7.49 6.18 6.03 6.22 7.30 6.48hsa-asg-3998 hsa-asg-3998_st1 10.40 8.62 8.53 8.70 9.41 8.74 8.32 9.20hsa-asg-9629 hsa-asg-9629_st2 6.42 4.93 5.35 4.21 4.51 5.11 4.96 3.98hsa-miR-9* hsa-miR-9-_st2 7.20 6.19 6.05 5.57 6.10 5.37 5.81 4.92hsa-asg-8948 hsa-asg-8948_st2 5.69 4.29 4.31 2.40 4.44 4.36 4.25 3.57hsa-asg-13598 hsa-asg-13598_st1 7.20 5.58 6.43 4.96 5.53 6.03 6.27 4.31hsa-asg-7131 hsa-asg-7131_st1 8.57 6.24 7.21 6.71 7.29 6.60 7.16 6.28hsa-cand960 hsa-cand960_st2 6.50 4.97 5.80 4.25 4.67 5.28 5.04 4.76hsa-asg-2237 hsa-asg-2237_st1 6.02 4.93 4.66 3.90 4.82 4.24 3.89 4.12hsa-asg-10426 hsa-asg-10426_st1 6.72 5.27 6.07 4.54 4.59 4.82 5.70 4.18hsa-asg-1591 hsa-asg-1591_st2 6.37 4.79 5.50 4.01 4.87 4.52 4.38 4.57hsa-asg-2042 hsa-asg-2042_st2 6.89 4.88 5.91 4.38 5.17 4.84 5.28 5.00hsa-asg-1815 hsa-asg-1815_st1 7.80 6.01 6.70 5.09 6.19 7.04 6.69 5.38hsa-asg-2342 hsa-asg-2342_st2 8.58 6.92 7.60 6.15 6.71 7.09 7.65 6.12hsa-asg-13316 hsa-asg-13316_st1 6.41 4.00 5.74 3.90 4.56 4.66 5.49 4.52hsa-asg-4007 hsa-asg-4007_st2 5.41 3.31 4.25 3.66 4.02 3.89 4.25 2.90hsa-asg-999 hsa-asg-999_st2 6.26 3.91 4.66 4.99 4.80 4.80 4.05 4.52hsa-asg-18 hsa-asg-18_st1 6.77 5.81 5.78 4.34 4.96 4.90 5.94 4.76hsa-asg-2542 hsa-asg-2542_st1 6.47 4.69 4.83 3.72 4.62 5.16 5.21 4.96hsa-asg-8636 hsa-asg-8636_st2 7.73 5.84 6.86 5.43 5.53 6.21 6.65 5.64hsa-asg-11900 hsa-asg-11900_st1 7.29 5.34 6.05 5.37 5.50 6.18 5.07 6.12hsa-asg-8617 hsa-asg-8617_st1 10.31 7.74 9.23 8.02 9.09 8.38 8.78 8.38hsa-asg-8188 hsa-asg-8188_st1 8.60 7.37 8.04 6.15 6.88 7.25 7.06 6.32hsa-asg-3587 hsa-asg-3587_st1 6.52 5.24 5.63 4.25 5.33 5.58 5.02 4.42hsa-asg-12814 hsa-asg-12814_st2 5.23 3.42 4.55 2.63 4.25 3.64 4.38 3.38hsa-asg-12146 hsa-asg-12146_st2 7.10 5.44 5.96 4.90 5.47 5.75 5.77 4.84hsa-asg-2318 hsa-asg-2318_st1 8.27 6.54 7.57 6.25 6.23 6.82 7.24 6.00hsa-asg-5875 hsa-asg-5875_st2 4.42 2.90 3.86 2.40 1.90 2.65 2.94 2.62hsa-asg-8159 hsa-asg-8159_st1 7.57 5.47 6.55 4.96 5.80 6.07 6.47 6.12hsa-asg-10731 hsa-asg-10731_st1 5.55 4.53 3.78 3.66 3.75 4.86 3.58 3.48hsa-asg-13779 hsa-asg-13779_st2 6.57 4.53 5.80 4.65 5.09 4.73 5.43 4.31hsa-miR-566 hsa-miR-566_st1 7.45 6.72 6.12 5.12 5.83 6.11 6.10 5.55hsa-asg-9886 hsa-asg-9886_st2 8.08 5.90 7.21 5.76 5.63 6.76 7.19 6.21hsa-asg-9511 hsa-asg-9511_st2 8.23 6.96 7.27 6.18 6.81 7.12 7.02 5.88hsa-cand109 hsa-cand109_st1 8.57 6.97 7.70 6.23 6.78 7.61 7.41 6.69hsa-asg-10971 hsa-asg-10971_st2 7.56 6.22 6.90 5.39 5.96 6.17 5.98 5.98hsa-asg-6422 hsa-asg-6422_st2 5.76 3.83 5.44 3.90 3.98 3.55 4.12 3.66hsa-asg-11500 hsa-asg-11500_st1 6.39 4.97 5.23 4.78 4.87 4.44 5.11 4.18hsa-asg-8121 hsa-asg-8121_st2 7.00 4.74 6.23 5.31 4.96 5.54 5.49 5.10hsa-asg-5880 hsa-asg-5880_st1 5.96 4.64 5.78 3.72 4.17 4.44 3.97 4.12hsa-asg-3714 hsa-asg-3714_st1 7.79 6.91 6.48 5.24 5.86 6.97 6.44 5.98hsa-asg-11228 hsa-asg-11228_st1 9.00 7.31 8.32 6.80 7.06 7.21 7.93 7.07hsa-asg-80 hsa-asg-80_st1 6.30 4.53 5.12 4.42 4.87 4.86 4.19 4.18hsa-asg-11990 hsa-asg-11990_st1 6.62 4.59 5.54 4.71 5.17 4.90 6.17 4.18hsa-asg-14051 hsa-asg-14051_st2 8.10 6.09 7.27 5.94 5.91 6.88 7.07 6.09hsa-asg-9399 hsa-asg-9399_st2 6.73 5.40 5.72 4.54 4.85 5.72 5.18 4.88hsa-asg-6924 hsa-asg-6924_st1 8.07 6.53 7.33 6.10 7.10 6.12 6.92 6.35hsa-asg-10259 hsa-asg-10259_st1 7.09 5.13 6.34 5.19 5.22 6.50 5.60 4.88hsa-asg-2150 hsa-asg-2150_st2 5.30 3.83 4.61 3.78 3.55 3.93 4.22 4.12hsa-asg-7027 hsa-asg-7027_st2 6.88 5.47 6.08 5.12 5.29 5.07 6.14 4.80hsa-asg-8932 hsa-asg-8932_st1 5.68 4.69 4.90 3.90 3.38 3.81 4.19 3.57hsa-miR-422a hsa-miR-422a_st1 10.71 9.09 8.97 9.17 9.80 8.95 8.80 9.67hsa-asg-2468 hsa-asg-2468_st2 4.30 2.22 3.48 1.89 2.82 3.29 3.53 2.31hsa-asg-3347 hsa-asg-3347_st2 5.10 2.75 3.48 3.04 3.75 3.50 3.77 3.75hsa-asg-128 hsa-asg-128_st2 6.08 4.84 4.71 4.54 5.11 4.52 4.12 4.96hsa-asg-819 hsa-asg-819_st1 9.01 7.79 8.45 6.60 7.27 7.45 8.01 6.94hsa-asg-3503 hsa-asg-3503_st2 5.17 3.31 3.68 3.38 3.12 3.97 3.32 3.15hsa-asg-4971 hsa-asg-4971_st1 7.43 5.64 6.65 5.39 5.63 5.58 6.54 5.03hsa-asg-8115 hsa-asg-8115_st2 5.03 3.53 4.03 3.04 3.98 3.06 3.32 3.38hsa-cand892 hsa-cand892_st1 6.56 4.69 5.68 4.34 4.89 4.96 5.99 4.57hsa-asg-10007 hsa-asg-10007_st1 8.84 7.15 7.80 6.62 7.01 7.45 7.60 6.98hsa-asg-8565 hsa-asg-8565_st1 7.80 6.55 7.29 5.69 6.16 6.72 6.99 5.62hsa-asg-4372 hsa-asg-4372_st2 7.77 6.03 6.76 5.57 5.91 5.98 7.06 5.86hsa-asg-4843 hsa-asg-4843_st2 7.67 5.90 6.72 5.85 6.07 6.12 6.30 5.55hsa-asg-9870 hsa-asg-9870_st2 6.59 5.05 5.79 4.30 4.82 5.58 5.67 4.62hsa-asg-6183 hsa-asg-6183_st1 7.95 6.20 7.28 5.76 6.32 6.78 7.19 5.80hsa-asg-7429 hsa-asg-7429_st1 5.74 3.73 5.38 3.78 3.66 4.24 4.60 4.25hsa-asg-10805 hsa-asg-10805_st1 8.42 7.52 7.33 6.30 6.99 7.21 7.30 5.76hsa-asg-105 hsa-asg-105_st1 5.71 3.73 4.94 3.66 3.94 4.80 4.19 3.57hsa-asg-1997 hsa-asg-1997_st1 6.31 4.53 5.35 4.11 4.02 4.84 5.34 4.71hsa-asg-2592 hsa-asg-2592_st1 7.97 6.50 7.12 5.55 6.41 6.78 6.72 6.42hsa-asg-11943 hsa-asg-11943_st1 6.64 5.24 5.75 4.96 4.87 5.05 6.00 4.80hsa-asg-12681 hsa-asg-12681_st1 10.59 9.39 9.67 8.50 8.88 9.36 9.45 8.31hsa-asg-7463 hsa-asg-7463_st1 7.02 5.76 5.92 5.26 5.26 5.33 5.78 5.36hsa-asg-4715 hsa-asg-4715_st1 5.67 3.83 5.06 3.04 4.17 4.30 4.54 3.98hsa-asg-5032 hsa-asg-5032_st2 9.45 8.73 8.68 7.37 7.60 8.41 8.30 7.13hsa-asg-3064 hsa-asg-3064_st2 5.05 3.18 4.25 3.04 3.71 3.59 3.81 2.62hsa-asg-4100 hsa-asg-4100_st1 6.49 4.74 5.25 5.14 4.62 5.21 5.57 4.80hsa-asg-7214 hsa-asg-7214_st1 6.53 4.35 5.94 4.65 4.56 5.22 5.70 5.10hsa-asg-4001 hsa-asg-4001_st1 5.19 3.53 4.18 2.84 3.98 3.73 4.05 3.03hsa-asg-8687 hsa-asg-8687_st1 8.25 6.37 7.78 5.82 6.51 7.19 7.07 6.30hsa-asg-6569 hsa-asg-6569_st2 5.48 4.64 4.11 3.78 3.50 4.24 4.25 3.48hsa-asg-7053 hsa-asg-7053_st2 7.45 5.52 6.79 5.33 5.62 5.93 6.19 5.80hsa-asg-3694 hsa-asg-3694_st1 7.33 6.27 6.68 5.43 5.09 5.51 6.02 5.36hsa-asg-1762 hsa-asg-1762_st1 9.53 7.86 8.20 7.23 8.24 9.03 8.02 7.61hsa-asg-8655 hsa-asg-8655_st1 8.17 6.39 6.84 6.30 6.21 6.86 7.25 6.50hsa-asg-3156 hsa-asg-3156_st1 6.34 4.93 5.53 4.61 4.73 4.96 4.49 4.42hsa-asg-10448 hsa-asg-10448_st2 5.35 4.00 4.76 3.38 3.75 3.55 4.12 3.38hsa-asg-7170 hsa-asg-7170_st2 5.35 4.22 3.78 3.21 4.10 3.81 4.70 3.48hsa-asg-13296 hsa-asg-13296_st2 5.40 3.91 4.85 3.38 3.89 4.30 4.12 4.05hsa-asg-12979 hsa-asg-12979_st1 7.56 5.81 7.02 5.57 6.01 6.45 6.64 5.33hsa-asg-9409 hsa-asg-9409_st1 6.80 5.47 5.40 5.51 5.71 5.17 5.42 4.31hsa-asg-7475 hsa-asg-7475_st1 5.46 3.53 4.11 3.78 4.21 4.57 3.72 3.57hsa-asg-12893 hsa-asg-12893_st2 5.51 3.73 4.71 3.66 3.98 4.11 4.83 3.15hsa-asg-8311 hsa-asg-8311_st1 6.10 5.58 3.95 4.54 4.85 4.80 4.88 4.42hsa-asg-4031 hsa-asg-4031_st2 9.40 8.14 8.64 7.15 7.73 8.26 8.27 7.59hsa-asg-3941 hsa-asg-3941_st2 8.18 6.26 7.55 5.82 6.57 7.23 7.44 6.35hsa-asg-8068 hsa-asg-8068_st2 7.63 6.73 6.65 5.59 5.96 6.42 6.58 5.86hsa-miR203 hsa-miR203_st1 7.51 6.05 6.89 5.91 5.49 5.97 6.40 5.74hsa-asg-773 hsa-asg-773_st1 8.29 7.21 7.41 6.30 6.35 7.33 6.73 6.33hsa-asg-9117 hsa-asg-9117_st1 6.97 5.50 6.36 5.21 5.52 5.33 6.28 5.38hsa-asg-8946 hsa-asg-8946_st1 5.84 4.22 4.98 4.01 3.85 4.71 5.21 3.57hsa-miR-510 hsa-miR-510_st2 6.20 5.27 5.02 4.75 4.65 4.71 5.04 4.52hsa-asg-7130 hsa-asg-7130_st1 6.77 4.93 6.25 4.65 5.34 5.51 6.13 4.67hsa-asg-11652 hsa-asg-11652_st1 7.02 5.76 6.35 4.65 5.50 5.94 6.13 4.92hsa-asg-9484 hsa-asg-9484_st2 8.80 7.05 8.01 6.86 6.79 7.55 7.50 6.91hsa-asg-4863 hsa-asg-4863_st1 6.49 5.17 5.40 4.34 5.28 5.34 5.04 4.62hsa-asg-7617 hsa-asg-7617_st1 8.62 7.59 7.81 6.79 7.34 7.25 7.58 6.99hsa-asg-12512 hsa-asg-12512_st1 6.03 5.27 4.50 4.61 4.25 5.03 4.74 4.36hsa-asg-8682 hsa-asg-8682_st2 5.82 4.22 4.61 5.09 4.80 4.11 4.79 3.75hsa-asg-11747 hsa-asg-11747_st1 7.55 5.71 6.76 5.33 6.00 6.22 6.33 5.83hsa-cand6 hsa-cand6_st2 8.25 7.03 7.79 6.10 6.90 7.17 7.36 6.27hsa-miR165 hsa-miR165_st1 6.53 5.66 5.71 5.31 5.17 5.40 5.43 4.71hsa-asg-9167 hsa-asg-9167_st1 6.90 5.34 6.14 5.31 5.39 5.67 5.76 5.33hsa-asg-3335 hsa-asg-3335_st1 8.09 6.19 7.04 6.10 6.97 7.40 7.00 6.11hsa-cand764 hsa-cand764_st1 7.14 5.86 6.04 5.55 5.73 6.35 6.04 5.03hsa-asg-6339 hsa-asg-6339_st1 8.73 7.51 8.13 6.67 7.33 7.99 7.78 6.59hsa-asg-5740 hsa-asg-5740_st2 6.40 5.71 5.74 4.81 5.31 5.50 5.11 4.62hsa-asg-8508 hsa-asg-8508_st2 5.92 4.47 5.59 4.30 4.02 4.17 5.04 4.47hsa-asg-2712 hsa-asg-2712_st1 5.98 4.47 5.02 4.38 4.38 4.47 5.00 4.25hsa-asg-6892 hsa-asg-6892_st2 8.53 7.42 7.70 6.32 6.79 7.86 7.49 6.68hsa-asg-9710 hsa-asg-9710_st1 6.00 5.09 5.10 4.01 4.65 4.98 4.92 4.36hsa-asg-4246 hsa-asg-4246_st2 6.16 4.59 5.41 4.75 4.87 5.17 4.92 4.25hsa-asg-2114 hsa-asg-2114_st1 5.17 3.05 4.25 4.01 3.55 4.27 3.58 4.12hsa-asg-8160 hsa-asg-8160_st2 8.39 6.86 7.61 6.38 6.98 7.48 7.48 6.27hsa-asg-11563 hsa-asg-11563_st2 7.92 6.19 7.32 5.94 6.22 7.21 6.77 6.32hsa-asg-9946 hsa-asg-9946_st1 6.40 4.69 5.25 5.49 4.38 4.84 4.83 5.67hsa-asg-7240 hsa-asg-7240_st1 7.02 6.05 6.19 5.01 6.10 6.23 6.05 5.10hsa-asg-9648 hsa-asg-9648_st1 6.85 5.93 5.78 5.21 5.28 6.17 5.54 5.30hsa-miR-487a hsa-asg-6915_st1 4.98 4.07 3.58 3.90 3.38 3.85 3.72 3.66hsa-asg-7507 hsa-asg-7507_st2 5.80 3.73 4.50 4.46 4.32 4.47 5.07 4.05hsa-asg-1833 hsa-asg-1833_st1 5.17 3.64 4.50 3.04 4.32 4.38 3.68 3.91hsa-cand634 hsa-cand634_st1 9.54 8.27 9.00 7.49 8.10 8.48 8.90 7.80hsa-asg-5507 hsa-asg-5507_st2 5.51 3.91 4.98 3.78 4.06 3.85 4.60 4.18hsa-asg-3969 hsa-asg-3969_st1 7.41 5.88 6.75 5.57 5.85 6.35 6.73 5.88hsa-cand471 hsa-cand471_st1 9.96 9.36 9.21 8.23 8.55 8.85 9.23 7.92hsa-cand356 hsa-cand356_st1 6.39 4.84 5.38 4.99 5.41 5.00 5.28 4.76hsa-asg-6876 hsa-asg-6876_st2 6.52 4.69 5.41 5.41 5.28 5.21 5.31 5.07hsa-miR-422b hsa-miR-422b_st2 11.91 10.62 10.51 10.71 11.31 10.68 10.310.94 hsa-asg-14002 hsa-asg-14002_st1 6.81 4.93 5.94 5.69 5.55 5.83 5.265.27 hsa-asg-2864 hsa-asg-2864_st2 6.54 5.05 5.77 4.87 5.47 5.51 5.395.27 hsa-asg-12328 hsa-asg-12328_st1 4.13 2.90 3.68 2.84 2.90 3.18 2.322.62 hsa-cand753 hsa-cand753_st1 6.55 5.55 5.86 4.93 5.18 5.39 5.79 4.62hsa-asg-9513 hsa-asg-9513_st1 7.31 5.93 6.75 5.33 5.83 6.13 6.33 5.74hsa-asg-9021 hsa-asg-9021_st1 8.96 7.85 8.35 7.22 7.57 8.31 8.01 7.37hsa-asg-8954 hsa-asg-8954_st2 7.07 5.81 6.48 5.14 5.66 5.91 6.62 5.60hsa-asg-3653 hsa-asg-3653_st2 7.26 6.60 6.57 5.62 5.85 6.07 6.04 5.60hsa-asg-739 hsa-asg-739_st1 5.94 5.13 4.85 4.30 4.80 5.22 5.28 4.36hsa-asg-14038 hsa-asg-14038_st1 8.58 7.85 7.48 7.36 7.65 7.43 7.00 7.34hsa-asg-8549 hsa-asg-8549_st1 9.31 8.16 8.63 7.64 7.82 8.41 8.33 7.49hsa-asg-9209 hsa-asg-9209_st2 7.51 6.13 6.97 5.97 6.34 6.48 6.84 5.74hsa-asg-4564 hsa-asg-4564_st2 6.85 5.76 6.16 5.59 5.62 6.12 6.17 4.84hsa-asg-8995 hsa-asg-8995_st1 8.84 7.45 8.22 7.19 7.54 8.09 8.24 7.14hsa-asg-2862 hsa-asg-2862_st1 7.91 6.32 7.27 7.50 6.84 6.71 6.93 6.57hsa-asg-1233 hsa-asg-1233_st1 10.13 9.21 9.51 8.40 8.78 9.38 9.42 8.65hsa-asg-13791 hsa-asg-13791_st2 5.47 4.47 4.50 4.68 3.94 4.17 4.19 4.25hsa-asg-77552 hsa-asg-7755_st2 5.79 5.31 4.85 4.54 4.70 4.76 4.49 4.80hsa-miR-379 hsa-miR-379_st1 7.65 6.51 6.37 7.12 6.96 7.01 6.54 6.33hsa-asg-2230 hsa-asg-2230_st2 6.35 5.47 6.00 4.96 4.70 5.27 5.42 4.80hsa-asg-2014 hsa-asg-2014_st1 5.78 4.93 5.02 4.11 5.24 4.71 5.15 4.62hsa-asg-2284 hsa-asg-2284_st1 5.67 4.69 5.32 4.21 4.17 4.57 4.96 4.25hsa-asg-1717 hsa-asg-1717_st2 5.35 3.42 4.25 4.93 4.62 4.17 4.25 4.31hsa-asg-1431 hsa-asg-1431_st2 6.05 5.24 5.21 4.93 5.28 5.17 4.25 4.80hsa-asg-8529 hsa-asg-8529_st1 7.87 6.94 7.18 6.44 6.40 7.22 7.16 6.42hsa-asg-3722 hsa-asg-3722_st1 9.94 9.12 9.51 9.24 8.61 8.85 8.99 8.29LNneg MicroRNA 8 9 M SD % ttest log2Diff Fold hsa-miR-429 2.41 5.36 2.861.14 0 5.59E−04 −6.63 99.1 hsa-miR-375 4.61 6.37 4.27 1.82 0 1.00E−02−6.44 86.5 hsa-miR-215 5.09 5.20 5.69 0.71 33 6.57E−05 −5.64 49.9hsa-asg-9939 2.23 4.97 2.66 1.11 0 2.14E−03 −5.19 36.5 hsa-asg-7846 4.293.89 2.91 1.01 0 1.45E−03 −5.07 33.6 hsa-asg-6880 6.86 7.29 6.06 0.65 228.15E−05 −5.02 32.4 hsa-miR-200a 4.75 7.76 5.54 1.15 67 3.37E−03 −4.9831.6 hsa-miR-187 3.35 3.14 3.33 0.61 0 5.85E−05 −4.96 31.2 hsa-asg-141821.85 0.73 1.86 0.68 0 1.31E−04 −4.92 30.2 hsa-miR-200b 7.64 10.4 7.681.27 100 6.41E−03 −4.89 29.7 hsa-asg-6838 6.95 7.53 6.30 0.61 448.12E−05 −4.73 26.6 hsa-asg-924 2.74 3.50 3.38 0.89 0 1.16E−03 −4.6525.0 hsa-miR-141 3.80 4.51 4.16 0.69 0 3.64E−04 −4.31 19.8 hsa-asg-134983.46 3.14 3.34 1.63 0 3.64E−02 −4.30 19.7 hsa-asg-8330 4.32 5.90 4.700.87 11 1.69E−03 −4.23 18.7 hsa-miR206 4.66 6.05 4.49 1.09 22 7.53E−03−4.07 16.8 hsa-miR-200c 8.87 11.5 9.05 1.21 100 1.34E−02 −4.04 16.5hsa-asg-7637 3.61 5.24 3.78 0.77 0 1.23E−03 −3.95 15.5 hsa-asg-125524.61 6.27 4.23 0.86 11 2.72E−03 −3.88 14.7 hsa-cand317 1.85 2.51 2.400.92 0 4.19E−03 −3.83 14.2 hsa-asg-11715 1.46 3.36 1.90 0.74 0 1.75E−03−3.60 12.1 hsa-asg-6377 2.41 3.06 2.67 0.66 0 9.16E−04 −3.58 12.0hsa-asg-3595 3.88 5.16 3.26 1.09 0 1.44E−02 −3.56 11.8 hsa-miR-605 3.034.44 2.52 1.03 0 1.15E−02 −3.55 11.7 hsa-asg-9262 4.08 4.48 4.46 0.58 04.31E−04 −3.49 11.2 hsa-asg-11612 5.25 6.01 5.07 0.51 11 2.06E−04 −3.4511.0 hsa-miR-194 8.20 9.03 8.93 0.53 100 2.85E−04 −3.41 10.6 hsa-miR-8023.03 1.60 1.71 0.65 0 1.21E−03 −3.37 10.3 hsa-miR-802 2.58 1.83 2.100.82 0 7.93E−03 −3.05 8.3 hsa-asg-6027 10.1 10.1 8.75 0.89 44 7.13E−03−3.35 10.2 hsa-asg-4691 5.90 6.63 5.90 0.52 22 3.01E−04 −3.34 10.1hsa-miR-31 8.66 10.0 9.21 0.92 100 9.06E−03 −3.30 9.9 hsa-asg-3667 2.414.13 2.67 0.64 0 1.25E−03 −3.30 9.8 hsa-asg-12983 3.03 3.62 2.56 1.00 01.50E−02 −3.26 9.6 hsa-asg-3046 5.36 5.84 5.62 0.54 78 4.18E−04 −3.269.6 hsa-asg-9082 3.56 2.80 2.99 0.89 0 8.96E−03 −3.23 9.4 hsa-cand6013.40 2.89 2.98 0.86 0 7.45E−03 −3.22 9.3 hsa-asg-13298 5.48 6.20 5.720.50 44 3.06E−04 −3.19 9.1 hsa-miR-200a* 3.17 2.89 2.72 0.64 0 1.64E−03−3.16 8.9 hsa-asg-2154 3.29 3.22 2.24 0.94 0 1.32E−02 −3.15 8.9hsa-asg-12799 4.29 4.84 4.19 0.87 0 1.15E−02 −3.00 8.0 hsa-asg-137053.17 3.22 2.27 1.11 0 3.46E−02 −2.97 7.8 hsa-asg-11160 3.51 5.11 3.210.79 0 7.52E−03 −2.95 7.7 hsa-asg-12058 3.88 1.60 2.38 0.94 0 1.96E−02−2.89 7.4 hsa-asg-10481 2.89 4.29 3.29 0.73 0 5.72E−03 −2.87 7.3hsa-miR-192 8.79 9.63 9.64 0.67 100 3.84E−03 −2.85 7.2 hsa-asg-114102.23 2.89 2.53 0.78 0 8.86E−03 −2.84 7.2 hsa-asg-11320 3.97 4.21 4.220.31 0 2.66E−05 −2.82 7.0 hsa-asg-10131 3.17 5.07 3.22 0.79 0 9.48E−03−2.82 7.0 hsa-asg-12139 2.05 3.50 2.32 0.59 0 1.96E−03 −2.80 7.0hsa-asg-11926 4.25 3.22 2.55 0.95 0 2.36E−02 −2.79 6.9 hsa-asg-130305.05 4.13 4.30 0.51 0 8.82E−04 −2.76 6.8 hsa-asg-5986 7.41 8.39 7.180.75 0 9.04E−03 −2.72 6.6 hsa-cand899 4.22 5.09 4.04 0.78 0 1.08E−02−2.71 6.5 hsa-asg-5119 2.58 3.62 2.49 0.55 0 1.71E−03 −2.70 6.5hsa-asg-7095 6.02 6.41 5.49 0.51 22 1.05E−03 −2.68 6.4 hsa-asg-4219 2.743.94 2.43 0.87 0 1.98E−02 −2.67 6.4 hsa-asg-4066 3.61 5.40 3.63 1.06 04.44E−02 −2.66 6.3 hsa-miR-9 1.66 3.62 2.79 0.63 0 3.86E−03 −2.65 6.3hsa-miR-9 3.29 4.13 3.29 0.55 0 2.02E−03 −2.60 6.1 hsa-asg-8239 3.294.13 3.22 0.68 0 6.31E−03 −2.64 6.3 hsa-asg-10411 3.71 2.07 3.20 0.84 01.77E−02 −2.64 6.2 hsa-asg-4015 5.47 5.45 5.21 0.92 0 2.67E−02 −2.62 6.1hsa-asg-3036 4.73 4.67 3.65 0.96 0 3.32E−02 −2.61 6.1 hsa-asg-1260 2.411.60 2.60 0.48 0 8.69E−04 −2.61 6.1 hsa-asg-7262 5.82 6.05 5.50 0.59 563.13E−03 −2.60 6.1 hsa-cand830 3.17 3.50 2.85 0.81 0 1.69E−02 −2.57 5.9hsa-asg-9752 5.57 5.28 4.63 0.52 0 1.50E−03 −2.57 5.9 hsa-asg-7014 3.934.08 3.78 0.97 0 3.74E−02 −2.55 5.9 hsa-asg-2551 5.32 6.57 5.06 1.01 114.38E−02 −2.54 5.8 hsa-asg-4870 4.63 4.41 3.91 0.94 0 3.38E−02 −2.53 5.8hsa-asg-4685 4.32 4.76 3.82 0.65 0 6.26E−03 −2.53 5.8 hsa-asg-6071 4.384.13 3.58 0.70 0 9.42E−03 −2.50 5.7 hsa-asg-3521 2.74 2.89 2.42 0.79 01.72E−02 −2.50 5.7 hsa-asg-12755 3.61 5.57 4.13 0.95 22 3.66E−02 −2.505.6 hsa-asg-12625 3.46 3.29 3.79 0.67 0 8.12E−03 −2.46 5.5 hsa-asg-27385.07 6.36 5.24 0.74 33 1.36E−02 −2.46 5.5 hsa-asg-12941 8.99 7.44 8.210.68 100 9.49E−03 −2.43 5.4 hsa-miR-381 1.28 3.22 3.08 0.91 0 3.68E−02−2.41 5.3 hsa-asg-12590 3.80 3.36 3.25 0.78 0 1.90E−02 −2.41 5.3hsa-asg-6773 2.23 2.51 2.64 0.64 0 7.55E−03 −2.41 5.3 hsa-asg-13797 4.554.13 3.37 0.67 0 9.49E−03 −2.41 5.3 hsa-asg-8922 7.54 7.77 7.26 0.77 1001.89E−02 −2.39 5.3 hsa-asg-1689 5.32 5.77 5.41 0.36 11 2.32E−04 −2.395.2 hsa-asg-11219 2.58 4.92 3.51 0.97 0 4.99E−02 −2.36 5.2 hsa-asg-134503.71 3.22 2.84 0.77 0 2.00E−02 −2.36 5.1 hsa-asg-12365 5.43 5.97 5.110.86 22 3.21E−02 −2.36 5.1 hsa-asg-933 8.21 7.68 7.23 0.57 0 4.53E−03−2.35 5.1 hsa-asg-8174 2.74 4.13 3.21 0.53 0 3.02E−03 −2.34 5.1hsa-asg-9574 6.54 6.00 5.84 0.77 44 2.01E−02 −2.34 5.1 hsa-asg-8397 3.514.70 3.62 0.90 0 3.90E−02 −2.34 5.1 hsa-asg-9319 4.97 5.79 4.52 0.76 01.99E−02 −2.32 5.0 hsa-asg-4080 3.29 3.06 2.83 0.74 0 1.77E−02 −2.31 5.0hsa-asg-3900 3.17 0.73 2.83 0.83 0 2.95E−02 −2.30 4.9 hsa-asg-11056 2.415.07 3.35 0.87 0 3.59E−02 −2.30 4.9 hsa-asg-4915 2.05 4.44 2.96 0.81 02.73E−02 −2.30 4.9 hsa-asg-6500 5.09 5.47 4.77 0.59 0 6.37E−03 −2.28 4.9hsa-asg-3716 5.78 5.56 5.11 0.76 0 2.20E−02 −2.28 4.8 hsa-asg-1351 4.444.92 4.00 0.76 11 2.14E−02 −2.27 4.8 hsa-asg-7385 5.63 5.59 5.34 0.35 222.64E−04 −2.27 4.8 hsa-asg-9879 3.40 3.06 3.19 0.63 0 9.53E−03 −2.25 4.8hsa-asg-6381 3.40 4.64 3.85 0.76 0 2.31E−02 −2.25 4.7 hsa-miR-124a 3.404.82 3.54 0.87 0 4.14E−02 −2.23 4.7 hsa-asg-8986 5.22 6.22 5.00 0.75 02.26E−02 −2.23 4.7 hsa-asg-10457 4.04 4.64 3.61 0.85 0 3.92E−02 −2.224.7 hsa-asg-11656 4.82 5.04 4.66 0.43 0 1.17E−03 −2.20 4.6 hsa-asg-95732.74 4.13 3.02 0.61 0 9.37E−03 −2.20 4.6 hsa-asg-8602 5.83 6.38 5.570.61 11 8.90E−03 −2.19 4.6 hsa-asg-3061 6.20 5.54 5.47 0.54 33 5.08E−03−2.19 4.6 hsa-asg-6135 2.41 5.16 4.13 0.83 0 3.60E−02 −2.19 4.6hsa-asg-8972 3.17 4.13 3.04 0.86 0 4.24E−02 −2.19 4.5 hsa-asg-8703 3.714.70 3.36 0.80 0 3.24E−02 −2.18 4.5 hsa-asg-8522 4.75 4.76 4.33 0.47 02.17E−03 −2.18 4.5 hsa-asg-6116 2.74 1.83 2.45 0.54 0 5.24E−03 −2.18 4.5hsa-asg-9781 5.46 6.18 5.06 0.76 11 2.68E−02 −2.17 4.5 hsa-asg-1727 5.115.68 4.97 0.77 11 2.77E−02 −2.17 4.5 hsa-asg-2894 3.61 4.13 3.21 0.74 02.36E−02 −2.16 4.5 hsa-asg-12922 4.75 4.92 4.43 0.53 0 4.92E−03 −2.164.5 hsa-asg-1715 3.40 3.06 3.25 0.58 0 7.66E−03 −2.15 4.4 hsa-asg-105807.76 9.33 8.19 0.62 100 1.10E−02 −2.15 4.4 hsa-asg-7183 3.61 4.37 3.710.50 0 3.73E−03 −2.14 4.4 hsa-asg-11797 5.47 6.15 5.47 0.83 33 3.99E−02−2.13 4.4 hsa-asg-13091 6.30 7.24 6.25 0.83 78 4.17E−02 −2.13 4.4hsa-asg-12684 8.14 8.25 7.97 0.32 100 2.46E−04 −2.12 4.4 hsa-asg-38417.20 6.93 6.48 0.54 89 5.74E−03 −2.12 4.3 hsa-asg-7228 4.35 5.59 5.220.76 0 3.06E−02 −2.11 4.3 hsa-asg-8643 5.40 4.58 4.87 0.62 0 1.19E−02−2.11 4.3 hsa-asg-1296 3.88 4.13 3.24 0.85 0 4.71E−02 −2.11 4.3hsa-asg-5662 3.66 5.09 4.39 0.81 11 4.04E−02 −2.10 4.3 hsa-asg-6614 4.325.40 4.32 0.85 0 4.85E−02 −2.09 4.2 hsa-asg-12119 3.71 5.44 4.29 0.62 111.32E−02 −2.09 4.2 hsa-asg-9053 5.90 5.62 5.85 0.69 33 2.07E−02 −2.084.2 hsa-asg-9270 2.74 3.06 2.79 0.74 0 2.82E−02 −2.08 4.2 hsa-cand8943.80 3.73 3.43 0.70 0 2.32E−02 −2.08 4.2 hsa-asg-1513 4.53 5.04 4.730.72 0 2.62E−02 −2.07 4.2 hsa-asg-191 3.17 4.82 3.71 0.58 0 9.73E−03−2.06 4.2 hsa-asg-3815 5.01 5.61 4.51 0.81 0 4.28E−02 −2.06 4.2hsa-asg-10181 5.13 5.79 5.20 0.62 11 1.37E−02 −2.05 4.1 hsa-asg-126974.04 4.04 3.92 0.34 0 4.41E−04 −2.04 4.1 hsa-asg-6269 6.39 6.48 5.410.82 33 4.59E−02 −2.04 4.1 hsa-asg-10114 3.40 3.06 4.12 0.73 0 2.87E−02−2.04 4.1 hsa-asg-10399 3.88 4.21 3.95 0.42 0 1.74E−03 −2.03 4.1hsa-asg-13266 2.89 3.22 3.14 0.58 0 1.01E−02 −2.03 4.1 hsa-asg-6000 5.255.30 4.90 0.53 11 6.51E−03 −2.03 4.1 hsa-asg-7166 3.17 4.37 3.29 0.56 08.68E−03 −2.03 4.1 hsa-asg-466 5.28 5.09 4.59 0.79 0 4.24E−02 −2.02 4.1hsa-asg-6934 3.40 3.22 3.14 0.70 0 2.61E−02 −2.02 4.0 hsa-asg-265 5.775.36 5.97 0.74 44 3.24E−02 −2.01 4.0 hsa-asg-13334 2.89 2.71 2.99 0.41 01.57E−03 −2.01 4.0 hsa-asg-5712 6.82 7.18 6.23 0.56 67 9.55E−03 −2.014.0 hsa-asg-4829 9.60 10.6 9.16 0.82 89 4.98E−02 −2.00 4.0 hsa-asg-15964.19 4.37 4.20 0.49 0 4.65E−03 −2.00 4.0 hsa-asg-7278 4.73 5.42 5.020.58 11 1.12E−02 −2.00 4.0 hsa-miR-202 5.46 4.82 4.84 0.57 0 1.08E−02−2.00 4.0 hsa-asg-244 4.68 5.49 4.63 0.66 0 2.13E−02 −1.98 4.0hsa-asg-3614 4.73 4.61 4.49 0.59 0 1.30E−02 −1.98 4.0 hsa-asg-3289 3.173.94 3.49 0.56 0 9.64E−03 −1.98 4.0 hsa-asg-11361 2.58 4.29 3.39 0.52 06.76E−03 −1.98 3.9 hsa-asg-8953 8.34 8.97 7.81 0.76 100 3.94E−02 −1.973.9 hsa-asg-572 4.58 5.34 4.44 0.74 0 3.46E−02 −1.97 3.9 hsa-asg-117965.70 5.97 5.43 0.48 11 4.84E−03 −1.97 3.9 hsa-cand630 5.03 5.26 5.080.66 0 2.25E−02 −1.97 3.9 hsa-asg-9085 5.58 6.11 5.50 0.78 11 4.48E−02−1.97 3.9 hsa-asg-3150 5.87 5.59 6.15 0.62 56 1.69E−02 −1.96 3.9hsa-asg-6734 2.58 3.22 2.86 0.61 0 1.66E−02 −1.96 3.9 hsa-asg-1010 4.754.70 4.71 0.69 11 2.73E−02 −1.95 3.9 hsa-asg-7067 4.44 5.07 4.48 0.62 111.70E−02 −1.95 3.9 hsa-asg-11875 4.19 4.92 3.87 0.74 0 3.64E−02 −1.953.9 hsa-asg-9320 4.80 5.28 4.79 0.60 11 1.51E−02 −1.95 3.9 hsa-asg-98733.51 3.36 2.95 0.71 0 3.08E−02 −1.94 3.9 hsa-asg-5115 7.52 7.44 7.150.52 100 7.57E−03 −1.94 3.8 hsa-asg-12556 7.29 6.90 7.66 0.76 784.16E−02 −1.93 3.8 hsa-asg-807 4.97 5.54 4.86 0.52 11 8.15E−03 −1.93 3.8hsa-asg-9025 6.05 6.16 5.22 0.62 11 1.80E−02 −1.92 3.8 hsa-asg-126522.23 3.22 2.80 0.63 0 2.08E−02 −1.92 3.8 hsa-asg-9547 4.25 5.32 3.970.72 0 3.48E−02 −1.92 3.8 hsa-asg-5385 5.66 5.86 5.80 0.40 33 1.93E−03−1.92 3.8 hsa-asg-13661 5.28 5.26 5.03 0.70 0 3.25E−02 −1.92 3.8hsa-asg-7178 5.83 5.62 5.06 0.75 0 4.15E−02 −1.91 3.8 hsa-cand173 6.125.56 6.08 0.65 56 2.35E−02 −1.91 3.8 hsa-asg-1981 3.40 4.37 3.59 0.63 02.02E−02 −1.91 3.7 hsa-asg-477 5.30 6.44 5.18 0.73 22 3.83E−02 −1.90 3.7hsa-asg-11742 4.25 4.51 3.90 0.76 0 4.52E−02 −1.89 3.7 hsa-asg-7776 4.534.73 4.59 0.61 0 1.90E−02 −1.89 3.7 hsa-asg-7215 5.40 4.97 5.32 0.47 114.99E−03 −1.89 3.7 hsa-asg-4814 6.42 6.22 6.24 0.58 78 1.49E−02 −1.893.7 hsa-asg-9163 4.38 4.29 3.86 0.71 0 3.49E−02 −1.89 3.7 hsa-asg-94536.75 5.76 6.94 0.77 33 4.91E−02 −1.88 3.7 hsa-asg-8404 3.40 4.58 3.600.70 0 3.46E−02 −1.88 3.7 hsa-asg-8926 5.55 5.22 5.00 0.47 0 5.40E−03−1.88 3.7 hsa-asg-10962 3.03 4.51 3.35 0.71 0 3.64E−02 −1.88 3.7hsa-asg-225 5.76 5.22 5.57 0.48 22 6.28E−03 −1.88 3.7 hsa-asg-8512 4.324.44 4.02 0.46 0 5.12E−03 −1.87 3.7 hsa-asg-6941 4.55 5.44 4.44 0.64 02.40E−02 −1.87 3.7 hsa-asg-956 2.58 3.36 2.94 0.50 0 7.86E−03 −1.87 3.7hsa-asg-7344 4.55 5.11 4.46 0.47 0 5.22E−03 −1.87 3.6 hsa-cand624 7.307.60 6.83 0.71 78 3.74E−02 −1.86 3.6 hsa-asg-12925 7.72 6.68 7.44 0.67100 3.02E−02 −1.86 3.6 hsa-asg-12989 2.89 4.92 3.66 0.71 0 3.86E−02−1.86 3.6 hsa-asg-6618 5.18 5.22 5.37 0.49 22 7.23E−03 −1.86 3.6hsa-asg-3296 4.50 4.58 4.17 0.65 0 2.76E−02 −1.85 3.6 hsa-asg-3003 4.254.58 4.00 0.75 0 4.77E−02 −1.84 3.6 hsa-asg-3155 5.54 5.47 5.35 0.28 442.37E−04 −1.84 3.6 hsa-miR-7 8.56 8.46 8.60 0.64 100 2.57E−02 −1.84 3.6hsa-asg-6792 6.08 6.50 6.06 0.63 44 2.36E−02 −1.84 3.6 hsa-asg-123955.41 5.77 5.64 0.64 44 2.54E−02 −1.84 3.6 hsa-asg-2045 6.78 7.28 6.400.65 89 2.85E−02 −1.83 3.6 hsa-asg-3745 6.75 6.61 6.27 0.59 11 1.91E−02−1.83 3.6 hsa-miR189 4.35 4.54 4.57 0.59 11 1.81E−02 −1.83 3.6hsa-asg-4767 5.03 5.26 4.63 0.57 0 1.67E−02 −1.82 3.5 hsa-asg-9849 6.557.02 6.68 0.74 89 5.00E−02 −1.81 3.5 hsa-asg-394 4.61 3.36 3.62 0.57 01.64E−02 −1.80 3.5 hsa-asg-4061 7.34 7.49 6.89 0.69 78 3.81E−02 −1.803.5 hsa-asg-8931 4.71 4.82 4.47 0.35 0 1.30E−03 −1.80 3.5 hsa-asg-40554.38 3.22 3.48 0.54 0 1.35E−02 −1.80 3.5 hsa-asg-6866 3.88 4.13 3.340.66 0 3.16E−02 −1.80 3.5 hsa-asg-10802 4.85 4.58 4.33 0.56 0 1.61E−02−1.80 3.5 hsa-asg-13197 3.03 3.50 2.87 0.47 0 6.60E−03 −1.79 3.5hsa-asg-4964 5.30 5.76 4.84 0.69 0 3.96E−02 −1.79 3.5 hsa-asg-242 5.055.02 4.65 0.53 0 1.25E−02 −1.78 3.4 hsa-asg-4693 5.65 5.54 5.14 0.51 111.11E−02 −1.78 3.4 hsa-asg-1127 2.41 3.73 3.54 0.54 0 1.43E−02 −1.78 3.4hsa-asg-1172 4.50 3.62 4.65 0.71 0 4.50E−02 −1.78 3.4 hsa-asg-12214 4.875.65 4.76 0.57 0 1.83E−02 −1.77 3.4 hsa-asg-1241 7.30 8.09 7.09 0.70 224.35E−02 −1.76 3.4 hsa-asg-10832 5.09 4.70 4.30 0.57 0 1.88E−02 −1.763.4 hsa-cand668 8.50 8.59 7.63 0.65 100 3.23E−02 −1.76 3.4 hsa-asg-79005.44 5.83 5.11 0.72 0 4.99E−02 −1.76 3.4 hsa-miR-518c* 3.71 4.64 4.020.65 0 3.46E−02 −1.75 3.4 hsa-asg-1576 7.43 7.63 7.51 0.46 100 6.83E−03−1.75 3.4 hsa-asg-3109 3.40 3.73 3.85 0.70 0 4.59E−02 −1.75 3.4hsa-asg-3771 4.75 4.04 4.30 0.53 0 1.43E−02 −1.75 3.4 hsa-asg-4440 4.684.95 5.46 0.51 22 1.18E−02 −1.74 3.3 hsa-asg-7671 6.17 5.30 6.26 0.67 784.03E−02 −1.73 3.3 hsa-asg-11795 5.75 4.82 5.44 0.59 22 2.30E−02 −1.733.3 hsa-asg-8731 5.36 4.76 4.45 0.52 0 1.31E−02 −1.73 3.3 hsa-asg-105104.32 4.76 3.87 0.61 0 2.74E−02 −1.73 3.3 hsa-asg-3744 5.46 4.64 4.870.71 11 4.93E−02 −1.72 3.3 hsa-asg-1716 5.53 5.42 4.83 0.52 0 1.45E−02−1.71 3.3 hsa-asg-4703 5.23 4.70 5.24 0.55 22 1.86E−02 −1.71 3.3hsa-asg-13135 4.44 5.57 5.11 0.44 22 6.03E−03 −1.70 3.3 hsa-asg-53266.55 5.32 6.29 0.63 67 3.39E−02 −1.70 3.3 hsa-asg-11898 4.93 5.36 4.720.54 11 1.78E−02 −1.70 3.2 hsa-miR-565 7.88 7.17 7.24 0.66 56 4.21E−02−1.69 3.2 hsa-asg-7224 6.69 6.78 6.86 0.67 89 4.32E−02 −1.69 3.2hsa-asg-3970 3.29 3.73 2.80 0.67 0 4.29E−02 −1.69 3.2 hsa-asg-9334 6.606.85 6.71 0.50 89 1.26E−02 −1.69 3.2 hsa-asg-92 4.19 5.11 4.48 0.61 113.04E−02 −1.68 3.2 hsa-asg-7174 6.63 6.39 6.06 0.64 44 3.84E−02 −1.673.2 hsa-asg-7133 5.83 6.77 5.69 0.66 33 4.23E−02 −1.67 3.2 hsa-asg-70234.85 4.29 4.36 0.26 0 2.94E−04 −1.66 3.2 hsa-asg-11096 6.64 7.22 6.590.62 89 3.50E−02 −1.66 3.2 hsa-asg-3998 8.74 8.47 8.75 0.35 100 1.97E−03−1.66 3.2 hsa-asg-9629 4.75 5.16 4.77 0.46 0 8.93E−03 −1.65 3.1hsa-miR-9* 4.71 5.20 5.55 0.53 44 1.92E−02 −1.65 3.1 hsa-asg-8948 4.324.44 4.04 0.67 0 4.78E−02 −1.65 3.1 hsa-asg-13598 5.47 5.38 5.55 0.66 224.51E−02 −1.64 3.1 hsa-asg-7131 7.49 7.39 6.93 0.48 33 1.17E−02 −1.643.1 hsa-cand960 4.63 4.41 4.87 0.47 11 1.12E−02 −1.63 3.1 hsa-asg-22374.19 4.70 4.38 0.40 0 4.61E−03 −1.63 3.1 hsa-asg-10426 5.48 5.16 5.090.61 44 3.49E−02 −1.63 3.1 hsa-asg-1591 4.44 5.64 4.75 0.53 11 1.93E−02−1.63 3.1 hsa-asg-2042 6.07 5.87 5.27 0.57 22 2.75E−02 −1.62 3.1hsa-asg-1815 6.20 6.31 6.18 0.63 78 3.97E−02 −1.62 3.1 hsa-asg-2342 7.037.34 6.96 0.56 89 2.45E−02 −1.62 3.1 hsa-asg-13316 5.23 5.07 4.80 0.6411 4.24E−02 −1.62 3.1 hsa-asg-4007 4.44 3.50 3.80 0.50 0 1.63E−02 −1.613.1 hsa-asg-999 4.89 5.20 4.65 0.42 0 7.01E−03 −1.61 3.0 hsa-asg-18 5.354.61 5.16 0.58 22 3.05E−02 −1.61 3.0 hsa-asg-2542 5.28 5.30 4.87 0.50 01.58E−02 −1.60 3.0 hsa-asg-8636 6.60 6.45 6.14 0.54 67 2.24E−02 −1.603.0 hsa-asg-11900 6.22 5.36 5.69 0.45 56 9.41E−03 −1.59 3.0 hsa-asg-86179.05 9.78 8.72 0.64 100 4.69E−02 −1.59 3.0 hsa-asg-8188 7.08 6.98 7.010.56 89 2.68E−02 −1.59 3.0 hsa-asg-3587 4.35 4.61 4.94 0.54 0 2.35E−02−1.59 3.0 hsa-asg-12814 3.17 3.36 3.64 0.63 0 4.42E−02 −1.58 3.0hsa-asg-12146 5.35 6.22 5.52 0.46 33 1.12E−02 −1.58 3.0 hsa-asg-23186.64 6.93 6.69 0.51 100 1.85E−02 −1.58 3.0 hsa-asg-5875 3.40 2.89 2.840.57 0 2.93E−02 −1.58 3.0 hsa-asg-8159 6.13 6.41 6.00 0.52 56 2.01E−02−1.58 3.0 hsa-asg-10731 3.71 4.44 3.98 0.50 0 1.70E−02 −1.57 3.0hsa-asg-13779 5.61 4.90 5.01 0.51 11 2.02E−02 −1.57 3.0 hsa-miR-566 6.025.42 5.89 0.47 56 1.37E−02 −1.57 3.0 hsa-asg-9886 6.88 7.05 6.51 0.64 674.81E−02 −1.57 3.0 hsa-asg-9511 6.36 6.48 6.67 0.47 100 1.37E−02 −1.562.9 hsa-cand109 7.10 6.68 7.02 0.48 100 1.58E−02 −1.55 2.9 hsa-asg-109716.43 5.04 6.01 0.54 78 2.66E−02 −1.55 2.9 hsa-asg-6422 4.50 4.97 4.220.63 11 4.99E−02 −1.54 2.9 hsa-asg-11500 4.99 5.04 4.85 0.34 0 2.43E−03−1.54 2.9 hsa-asg-8121 5.69 6.11 5.46 0.50 22 1.91E−02 −1.53 2.9hsa-asg-5880 4.08 4.90 4.43 0.62 11 4.70E−02 −1.53 2.9 hsa-asg-3714 6.925.61 6.27 0.63 78 4.94E−02 −1.53 2.9 hsa-asg-11228 7.68 7.87 7.47 0.50100 2.00E−02 −1.53 2.9 hsa-asg-80 5.18 5.59 4.77 0.48 0 1.67E−02 −1.532.9 hsa-asg-11990 5.50 5.14 5.10 0.59 11 4.02E−02 −1.52 2.9hsa-asg-14051 6.95 6.96 6.57 0.55 67 3.03E−02 −1.52 2.9 hsa-asg-93995.60 4.97 5.21 0.43 33 9.46E−03 −1.52 2.9 hsa-asg-6924 5.96 6.54 6.550.48 78 1.61E−02 −1.52 2.9 hsa-asg-10259 5.30 5.97 5.57 0.57 33 3.61E−02−1.52 2.9 hsa-asg-2150 3.51 2.51 3.78 0.59 0 4.00E−02 −1.52 2.9hsa-asg-7027 4.95 5.38 5.37 0.47 0 1.60E−02 −1.51 2.8 hsa-asg-8932 4.754.37 4.17 0.54 0 3.02E−02 −1.51 2.8 hsa-miR-422a 9.22 9.12 9.20 0.33 1002.54E−03 −1.51 2.8 hsa-asg-2468 2.41 3.22 2.80 0.61 0 4.70E−02 −1.50 2.8hsa-asg-3347 3.71 4.64 3.60 0.53 0 2.71E−02 −1.50 2.8 hsa-asg-128 4.324.13 4.58 0.35 0 3.75E−03 −1.50 2.8 hsa-asg-819 7.70 7.36 7.51 0.56 1003.42E−02 −1.50 2.8 hsa-asg-3503 4.66 4.44 3.67 0.57 0 3.60E−02 −1.50 2.8hsa-asg-4971 6.32 6.57 5.93 0.60 44 4.46E−02 −1.50 2.8 hsa-asg-8115 3.513.94 3.53 0.38 0 5.74E−03 −1.50 2.8 hsa-cand892 4.91 5.52 5.06 0.55 03.28E−02 −1.49 2.8 hsa-asg-10007 7.78 7.69 7.34 0.42 56 9.61E−03 −1.492.8 hsa-asg-8565 5.98 5.83 6.32 0.60 67 4.62E−02 −1.49 2.8 hsa-asg-43726.11 7.26 6.28 0.59 89 4.48E−02 −1.48 2.8 hsa-asg-4843 6.39 6.76 6.180.40 67 7.95E−03 −1.48 2.8 hsa-asg-9870 4.61 5.51 5.11 0.55 11 3.32E−02−1.48 2.8 hsa-asg-6183 6.60 6.28 6.47 0.54 56 3.28E−02 −1.48 2.8hsa-asg-7429 4.19 4.51 4.26 0.54 0 3.10E−02 −1.48 2.8 hsa-asg-10805 7.186.87 6.94 0.57 100 3.86E−02 −1.48 2.8 hsa-asg-105 4.25 4.97 4.23 0.56 03.57E−02 −1.48 2.8 hsa-asg-1997 5.46 5.16 4.84 0.54 0 3.11E−02 −1.48 2.8hsa-asg-2592 6.43 6.57 6.50 0.42 89 1.08E−02 −1.48 2.8 hsa-asg-119434.58 5.26 5.17 0.46 22 1.59E−02 −1.47 2.8 hsa-asg-12681 9.38 9.14 9.120.46 78 1.69E−02 −1.47 2.8 hsa-asg-7463 6.15 5.18 5.56 0.35 11 4.18E−03−1.47 2.8 hsa-asg-4715 4.44 4.51 4.21 0.57 0 3.96E−02 −1.47 2.8hsa-asg-5032 7.70 7.93 7.98 0.58 100 4.25E−02 −1.46 2.8 hsa-asg-30643.88 4.21 3.59 0.55 0 3.43E−02 −1.46 2.8 hsa-asg-4100 5.09 4.82 5.030.30 0 1.83E−03 −1.46 2.8 hsa-asg-7214 5.15 4.95 5.07 0.52 11 2.81E−02−1.46 2.8 hsa-asg-4001 3.97 4.29 3.73 0.51 0 2.55E−02 −1.46 2.8hsa-asg-8687 7.05 7.05 6.79 0.59 89 4.70E−02 −1.46 2.8 hsa-asg-6569 3.514.70 4.02 0.48 0 2.07E−02 −1.45 2.7 hsa-asg-7053 6.14 6.72 6.00 0.51 782.66E−02 −1.45 2.7 hsa-asg-3694 6.40 6.15 5.88 0.55 56 3.64E−02 −1.452.7 hsa-asg-1762 8.02 8.59 8.09 0.53 100 3.14E−02 −1.44 2.7 hsa-asg-86556.91 7.32 6.73 0.40 100 9.57E−03 −1.44 2.7 hsa-asg-3156 5.23 5.24 4.900.38 11 6.87E−03 −1.44 2.7 hsa-asg-10448 4.25 4.04 3.91 0.45 0 1.66E−02−1.44 2.7 hsa-asg-7170 4.44 3.50 3.91 0.49 0 2.33E−02 −1.44 2.7hsa-asg-13296 2.89 4.29 3.97 0.56 0 4.20E−02 −1.44 2.7 hsa-asg-129795.99 6.29 6.12 0.53 78 3.40E−02 −1.44 2.7 hsa-asg-9409 5.50 5.83 5.370.44 0 1.51E−02 −1.43 2.7 hsa-asg-7475 4.75 4.04 4.03 0.43 0 1.33E−02−1.43 2.7 hsa-asg-12893 4.50 4.13 4.09 0.54 0 3.59E−02 −1.42 2.7hsa-asg-8311 4.93 4.13 4.67 0.49 11 2.39E−02 −1.42 2.7 hsa-asg-4031 8.447.66 7.99 0.48 100 2.33E−02 −1.42 2.7 hsa-asg-3941 6.82 6.88 6.77 0.5889 4.90E−02 −1.41 2.7 hsa-asg-8068 6.37 5.86 6.22 0.41 89 1.19E−02 −1.412.7 hsa-miR203 6.17 6.28 6.10 0.40 33 1.08E−02 −1.41 2.7 hsa-asg-7737.34 6.93 6.88 0.47 100 2.12E−02 −1.41 2.7 hsa-asg-9117 4.68 5.88 5.570.53 0 3.67E−02 −1.40 2.6 hsa-asg-8946 4.61 4.87 4.45 0.56 0 4.62E−02−1.39 2.6 hsa-miR-510 4.25 5.07 4.81 0.32 11 3.20E−03 −1.39 2.6hsa-asg-7130 5.44 5.49 5.38 0.57 22 4.98E−02 −1.39 2.6 hsa-asg-116526.00 5.42 5.63 0.56 22 4.83E−02 −1.39 2.6 hsa-asg-9484 7.79 8.33 7.420.55 100 4.44E−02 −1.38 2.6 hsa-asg-4863 5.15 5.68 5.11 0.41 0 1.25E−02−1.38 2.6 hsa-asg-7617 7.30 6.54 7.24 0.41 100 1.26E−02 −1.38 2.6hsa-asg-12512 4.85 4.44 4.67 0.33 0 4.86E−03 −1.36 2.6 hsa-asg-8682 4.614.21 4.46 0.42 0 1.57E−02 −1.35 2.6 hsa-asg-11747 6.74 6.82 6.19 0.52 113.93E−02 −1.35 2.6 hsa-cand6 6.43 7.12 6.91 0.55 89 4.82E−02 −1.34 2.5hsa-miR165 4.82 4.73 5.22 0.38 11 1.16E−02 −1.31 2.5 hsa-asg-9167 5.286.04 5.58 0.33 0 5.56E−03 −1.31 2.5 hsa-asg-3335 6.92 7.33 6.79 0.52 784.27E−02 −1.31 2.5 hsa-cand764 6.36 5.62 5.84 0.42 44 1.83E−02 −1.30 2.5hsa-asg-6339 7.56 7.30 7.43 0.53 100 4.88E−02 −1.30 2.5 hsa-asg-57404.25 4.87 5.10 0.51 22 4.16E−02 −1.30 2.5 hsa-asg-8508 4.50 5.07 4.620.50 11 4.01E−02 −1.30 2.5 hsa-asg-2712 4.93 5.20 4.68 0.36 0 8.43E−03−1.30 2.5 hsa-asg-6892 7.61 7.21 7.23 0.52 100 4.65E−02 −1.30 2.5hsa-asg-9710 4.71 4.51 4.70 0.37 22 9.93E−03 −1.29 2.5 hsa-asg-4246 5.304.58 4.87 0.38 0 1.19E−02 −1.29 2.4 hsa-asg-2114 3.61 4.51 3.88 0.46 02.98E−02 −1.29 2.4 hsa-asg-8160 7.28 7.63 7.11 0.52 100 4.57E−02 −1.292.4 hsa-asg-11563 6.74 7.05 6.64 0.49 78 3.93E−02 −1.28 2.4 hsa-asg-99465.20 5.79 5.13 0.47 0 3.38E−02 −1.28 2.4 hsa-asg-7240 5.58 5.42 5.750.48 11 3.63E−02 −1.27 2.4 hsa-asg-9648 5.63 5.45 5.59 0.32 22 5.95E−03−1.27 2.4 hsa-miR-487a 3.71 3.62 3.72 0.20 0 3.55E−04 −1.25 2.4hsa-asg-7507 4.89 5.40 4.54 0.51 0 4.86E−02 −1.25 2.4 hsa-asg-1833 3.514.29 3.92 0.49 0 4.17E−02 −1.25 2.4 hsa-cand634 8.30 8.32 8.30 0.48 1003.82E−02 −1.25 2.4 hsa-asg-5507 4.12 4.92 4.27 0.45 11 3.17E−02 −1.252.4 hsa-asg-3969 6.13 6.49 6.18 0.42 89 2.42E−02 −1.23 2.3 hsa-cand4718.82 8.46 8.74 0.49 100 4.45E−02 −1.23 2.3 hsa-cand356 5.30 5.67 5.180.30 0 5.02E−03 −1.21 2.3 hsa-asg-6876 5.43 6.02 5.31 0.35 0 1.16E−02−1.21 2.3 hsa-miR-422b 10.7 10.5 10.7 0.28 100 3.76E−03 −1.20 2.3hsa-asg-14002 5.53 6.57 5.62 0.47 11 4.40E−02 −1.19 2.3 hsa-asg-28645.13 5.67 5.35 0.29 11 4.97E−03 −1.19 2.3 hsa-asg-12328 2.97 3.06 2.940.37 0 1.68E−02 −1.19 2.3 hsa-cand753 5.33 5.61 5.36 0.40 33 2.36E−02−1.18 2.3 hsa-asg-9513 6.45 6.64 6.13 0.46 78 4.25E−02 −1.18 2.3hsa-asg-9021 7.88 7.54 7.79 0.40 100 2.28E−02 −1.17 2.3 hsa-asg-89545.65 6.20 5.90 0.47 67 4.46E−02 −1.17 2.2 hsa-asg-3653 6.49 6.04 6.100.38 78 2.06E−02 −1.16 2.2 hsa-asg-739 4.19 4.97 4.79 0.41 33 2.92E−02−1.16 2.2 hsa-asg-14038 7.48 7.30 7.43 0.23 78 1.69E−03 −1.15 2.2hsa-asg-8549 8.43 8.53 8.16 0.41 100 2.99E−02 −1.15 2.2 hsa-asg-92096.55 6.38 6.38 0.39 67 2.58E−02 −1.14 2.2 hsa-asg-4564 5.66 5.52 5.720.42 33 3.32E−02 −1.13 2.2 hsa-asg-8995 7.98 7.71 7.73 0.42 100 3.72E−02−1.11 2.2 hsa-asg-2862 6.67 6.53 6.81 0.37 22 2.33E−02 −1.09 2.1hsa-asg-1233 9.15 8.89 9.04 0.38 78 2.75E−02 −1.08 2.1 hsa-asg-137914.19 5.07 4.38 0.34 0 1.61E−02 −1.08 2.1 hsa-asg-77552 4.89 4.04 4.710.34 11 1.76E−02 −1.08 2.1 hsa-miR-379 6.13 6.20 6.58 0.37 100 2.49E−02−1.07 2.1 hsa-asg-2230 5.18 5.74 5.28 0.43 0 4.59E−02 −1.07 2.1hsa-asg-2014 4.66 4.04 4.72 0.42 0 4.45E−02 −1.06 2.1 hsa-asg-2284 4.894.76 4.65 0.39 0 3.59E−02 −1.03 2.0 hsa-asg-1717 4.61 4.37 4.33 0.42 04.69E−02 −1.03 2.0 hsa-asg-1431 5.33 5.07 5.03 0.34 0 2.10E−02 −1.02 2.0hsa-asg-8529 7.40 6.47 6.85 0.41 100 4.68E−02 −1.02 2.0 hsa-asg-37229.00 8.68 8.92 0.37 100 3.00E−02 −1.02 2.0 SD, standard deviation; %,percentage of samples expressing miRNA above background levels;Log2Diff, difference in VSN-transformed expression bewteen SI and LNnegsamples.

Example 38 Differentially-Expressed miRNAs, Most Commonly Observed,Between 21 Normal Tissue Types and Normal Lymph Nodes

The inventors compiled a listing of miRNAs representing those that wereobserved by microarray expression analysis to be the miRNAs mostfrequently differentially-expressed between each of 21 normal tissuetypes and normal lymph nodes. The listing describes miRNAs whosedifferential expression was characterized by a p-value<0.05 and a levelof expression at least 10-fold higher in a particular tissue compared tonormal lymph nodes. The presence of a “1” in the column indicates that adifferentially expressed miRNA fulfills the criteria described above fora particular tissue; whereas a “0” in the column indicates that themiRNA does not fulfill the criteria. The column on the right indicatesthe number of time a miRNA was identified as differentially expressedbetween the 21 normal tissues and normal lymph nodes. Among the mostcommon differentially expressed miRNAs identified, four (hsa-miR-200a,-200b, -200c, and -429) were identified as differentially expressedbetween fifteen different tissues and normal lymph nodes; hsa-miR-605and -187 were identified as differentially expressed between 14 or 13different tissues, respectively, and normal lymph nodes; five miRNAs(the predicted miRNA hsa-asg-7637-st2 and the verified miRNAshsa-miR-203, -375, -411, and -487b) were identified as differentiallyexpressed between 12 different tissues and normal lymph nodes; 5 miRNAs(two predicted miRNAs, hsa-asg-14153_st2, and -5021_st1 and threeverified miRNAs, hsa-miR-368, -493-5p, and -495) were identified 11times; and 4 miRNAs (hsa-miR-141, -154, -205, and 381) were identifiedas differentially expressed between 10 different tissues and normallymph nodes. In addition, a total of 229 miRNAs were founddifferentially expressed between only one particular tissue and normallymph nodes. These miRNAs may potentially be used to specificallyidentify or confirm the origin of the tissue present in a metastaticlymph node.

TABLE 37 Differentially-expressed miRNAS, most commonly observed,between each of 21 normal tissue types and normal lymph nodes. Probe IDTYR KID STO TES UTR SPL BRN TNG THY TRA SI hsa-miR-200a_st2 1 1 1 0 0 00 1 1 1 1 hsa-miR-200b_st1 1 1 1 0 0 0 0 1 1 1 1 hsa-miR-200c_st1 1 1 10 0 0 0 1 1 1 1 hsa-miR-429_st1 1 1 1 0 0 0 0 1 1 1 1 hsa-miR-605_st1 11 1 0 0 0 0 1 1 1 1 hsa-miR-187_st1 0 1 0 1 1 0 1 1 0 1 1hsa-asg-7637_st2 0 1 1 0 0 0 0 1 1 1 1 hsa-miR-203_st2 1 1 1 0 0 0 0 1 11 0 hsa-miR-375_st1 0 0 1 0 0 0 0 1 1 1 1 hsa-miR-411_st1 0 1 0 1 1 1 11 1 0 0 hsa-miR-487b_st2 0 0 0 1 1 1 1 1 1 0 0 hsa-asg-14153_st2 0 1 1 00 0 0 1 1 1 0 hsa-asg-5021_st1 0 0 0 1 1 1 1 1 0 0 0 hsa-miR-368_st2 1 00 1 1 1 0 1 1 0 0 hsa-miR-493-5p_st1 0 1 0 1 1 1 0 1 1 0 0hsa-miR-495_st1 0 0 0 1 1 1 1 1 1 1 0 hsa-miR-141_st1 1 1 1 0 0 0 0 1 01 1 hsa-miR-154_st2 0 1 0 1 1 1 1 1 0 0 0 hsa-miR-205_st1 0 0 1 0 0 0 01 1 1 0 hsa-miR-381_st2 1 0 0 1 1 1 1 1 0 0 0 hsa-asg-11715_st2 1 1 1 00 0 0 1 1 1 1 hsa-asg-13254_st1 0 0 0 1 1 1 1 1 0 1 0 hsa-asg-13404_st20 0 0 1 1 1 1 1 1 0 0 hsa-asg-924_st1 0 1 1 0 0 0 1 1 0 1 1hsa-asg-5617_st1 0 0 0 1 1 1 1 1 0 0 0 hsa-miR-539_st1 0 0 0 1 0 1 1 1 00 0 hsa-miR202_st2 0 0 0 1 1 1 1 0 1 0 0 hsa-asg-10049_st1 0 0 0 1 0 1 11 0 1 0 hsa-miR-127_st1 0 0 0 1 1 1 1 1 0 0 0 hsa-miR-133a_st1 1 0 0 0 10 0 1 0 0 0 hsa-miR-133b_st2 1 0 0 0 0 0 0 1 0 0 0 hsa-miR-299-5p_st2 00 0 1 1 0 1 1 0 0 0 hsa-miR-432_st2 0 0 0 1 0 1 1 0 0 0 0hsa-asg-10131_st2 0 0 1 0 0 0 0 1 0 1 0 hsa-asg-14182_st1 0 1 1 0 0 0 00 0 0 1 hsa-asg-7472_st2 0 0 0 1 0 1 1 1 0 0 0 hsa-cand206_st1 0 0 0 1 01 1 1 0 0 0 hsa-miR-204_st2 0 1 0 1 1 0 1 0 0 0 0 hsa-miR-215_st1 0 1 10 0 0 0 0 0 0 1 hsa-miR-383_st1 0 0 0 1 1 0 1 0 1 1 0 hsa-miR-424_st1 01 0 1 1 1 0 0 0 0 0 hsa-asg-13166_st2 0 0 0 1 0 1 1 0 0 0 0hsa-miR-134_st2 0 0 0 1 0 1 1 0 0 0 0 hsa-miR-192_st2 0 1 1 0 0 0 0 0 00 0 hsa-miR-194_st2 0 1 1 0 0 0 0 0 0 0 1 hsa-miR-206_st2 1 0 0 0 0 0 01 0 0 0 hsa-miR-338_st1 0 0 0 0 0 1 1 0 0 0 0 hsa-miR-379_st1 0 0 0 1 00 1 1 0 0 0 hsa-miR-410_st1 0 0 0 1 0 1 1 0 0 0 0 hsa-miR-542-5p_st2 0 10 1 0 1 0 0 0 0 0 hsa-asg-12139_st2 0 0 1 0 0 0 0 1 0 1 0hsa-asg-12227_st1 0 1 1 0 0 0 0 0 0 1 0 hsa-asg-13705_st1 0 0 1 0 0 0 01 0 1 0 hsa-asg-2027_st1 1 0 0 0 0 0 0 1 0 0 0 hsa-asg-2345_st2 1 0 0 00 0 0 1 0 0 0 hsa-asg-8428_st2 0 0 1 0 0 0 0 1 0 0 0 hsa-miR-184_st1 0 10 1 0 0 1 0 0 0 0 hsa-miR-189_st1 0 0 0 0 1 0 0 0 0 0 0hsa-miR-200a-_st2 0 1 1 0 0 0 0 0 0 1 0 hsa-miR-34c_st2 0 0 0 1 0 0 0 10 1 0 hsa-miR-376a_st2 0 0 0 0 1 0 0 0 0 0 0 hsa-miR-376b_st1 0 0 0 1 01 0 1 0 0 0 hsa-miR-382_st1 0 0 0 1 0 0 1 0 0 0 0 hsa-miR-409-3p_st1 0 00 1 0 1 1 0 0 0 0 hsa-miR-485-5p_st2 0 0 0 1 0 0 1 0 0 0 0hsa-miR-592_st1 0 0 1 0 0 0 1 0 0 0 0 hsa-miR-96_st1 0 0 1 0 0 0 0 0 1 00 hsa-miR-9_st2 0 1 0 0 1 0 1 0 0 0 0 hsa-miR23_st2 0 0 0 1 0 1 1 0 0 00 hsa-asg-1088_st1 0 1 0 1 0 0 0 0 1 0 0 hsa-asg-11443_st1 0 1 0 0 1 0 00 0 0 0 hsa-asg-1296_st1 0 0 0 0 0 0 0 1 0 1 0 hsa-asg-14208_st1 0 1 0 10 0 0 0 0 0 0 hsa-asg-14228_st2 0 1 0 0 1 0 0 0 0 0 0 hsa-asg-1521_st1 00 0 0 0 1 1 1 0 0 0 hsa-asg-1794_st1 0 0 0 0 0 0 0 1 0 0 0hsa-asg-4915_st1 1 1 0 0 0 0 0 0 0 0 0 hsa-asg-4978_st2 0 1 1 0 0 0 0 00 1 0 hsa-asg-5119_st1 0 0 1 0 0 0 0 0 0 1 0 hsa-asg-5638_st2 0 1 0 1 00 0 0 0 0 0 hsa-asg-8128_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-9696_st1 0 00 1 0 0 0 0 0 0 0 hsa-cand150_st2 0 0 0 1 0 0 0 0 0 1 0 hsa-miR-132_st10 0 0 0 1 0 1 0 0 0 0 hsa-miR-149_st2 0 0 0 0 0 0 1 0 1 0 0hsa-miR-31_st2 0 0 1 0 0 0 0 0 0 1 0 hsa-miR-369-5p_st2 0 0 0 1 0 0 0 00 0 0 hsa-miR-433_st2 0 0 0 1 0 0 1 0 0 0 0 hsa-miR-449_st2 0 0 0 1 0 00 0 0 1 0 hsa-miR-450_st2 0 0 0 1 0 1 0 0 0 0 0 hsa-miR-582_st1 0 1 0 01 0 0 0 0 0 0 hsa-miR-9-_st2 0 0 0 0 1 0 1 0 0 0 0 hsa-miR12_st1 1 0 0 00 0 0 1 0 0 0 hsa-miR144_st2 0 1 0 0 1 0 0 0 0 0 0 hsa-asg-10080_st1 0 00 0 0 0 0 0 0 1 0 hsa-asg-10202_st2 0 0 0 0 0 0 1 0 0 0 0hsa-asg-10247_st1 0 0 0 0 0 0 0 0 0 1 0 hsa-asg-10309_st1 1 0 0 1 0 0 00 0 0 0 hsa-asg-10393_st1 1 0 0 0 0 0 0 1 0 0 0 hsa-asg-10881_st1 0 0 00 0 1 1 0 0 0 0 hsa-asg-10883_st1 0 0 0 0 0 0 0 0 0 0 0hsa-asg-11088_st1 0 0 0 0 0 0 0 1 0 0 0 hsa-asg-11338_st2 0 0 0 1 0 0 00 0 0 0 hsa-asg-13135_st1 0 0 0 0 0 1 1 0 0 0 0 hsa-asg-13231_st1 0 0 00 0 0 0 0 0 1 0 hsa-asg-13284_st1 0 0 0 0 0 0 1 0 0 0 0hsa-asg-13922_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-14065_st2 0 0 0 0 1 0 01 0 0 0 hsa-asg-2399_st2 1 0 0 0 0 0 0 1 0 0 0 hsa-asg-2424_st1 0 0 1 00 0 0 0 0 1 0 hsa-asg-2484_st2 1 0 0 0 0 0 0 1 0 0 0 hsa-asg-3081_st1 10 0 1 0 0 0 0 0 0 0 hsa-asg-3822_st2 0 0 0 0 0 0 0 0 0 1 0hsa-asg-3983_st1 1 0 0 0 0 0 0 0 0 0 0 hsa-asg-4209_st2 1 0 0 0 0 0 0 00 0 0 hsa-asg-4219_st1 0 0 1 0 0 0 0 0 0 1 0 hsa-asg-4438_st2 0 0 0 0 00 0 0 0 1 0 hsa-asg-4439_st2 0 0 0 0 0 0 0 0 0 1 0 hsa-asg-5602_st2 0 00 0 0 0 0 0 0 0 0 hsa-asg-5630_st2 0 0 0 1 0 0 0 0 0 0 0hsa-asg-6283_st1 1 0 0 1 0 0 0 0 0 0 0 hsa-asg-7295_st2 0 0 0 1 0 0 1 00 0 0 hsa-asg-8480_st1 0 0 0 1 0 1 0 0 0 0 0 hsa-asg-8616_st2 0 0 0 1 00 0 0 0 0 0 hsa-asg-9198_st1 0 0 0 0 0 0 0 1 0 0 0 hsa-asg-9219_st2 0 01 0 0 0 0 0 0 0 0 hsa-asg-9262_st1 0 0 0 1 0 0 0 0 0 0 1hsa-asg-9269_st2 0 0 0 0 0 0 0 0 0 1 0 hsa-asg-9514_st1 0 0 0 1 0 0 1 00 0 0 hsa-asg-9687_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-9830_st2 0 0 0 0 00 0 0 0 1 0 hsa-cand14_st1 1 0 0 0 0 0 0 1 0 0 0 hsa-cand26_st2 0 0 0 00 1 1 0 0 0 0 hsa-cand317_st1 0 0 1 0 0 0 0 0 0 0 1 hsa-cand79_st1 0 1 00 0 0 1 0 0 0 0 hsa-miR-105_st1 0 0 0 1 0 0 1 0 0 0 0 hsa-miR-139_st2 00 0 0 0 1 1 0 0 0 0 hsa-miR-154-_st2 0 0 0 1 0 0 1 0 0 0 0hsa-miR-182_st1 0 0 0 0 0 0 0 0 1 0 0 hsa-miR-183_st1 0 0 0 0 0 0 0 0 10 0 hsa-miR-328_st1 0 0 0 0 0 0 1 0 0 0 0 hsa-miR-329_st1 0 0 0 1 0 0 00 0 0 0 hsa-miR-378_st1 0 0 0 0 0 0 0 1 0 0 0 hsa-miR-380-3p_st2 0 0 0 10 0 1 0 0 0 0 hsa-miR-409-5p_st1 0 0 0 1 0 0 1 0 0 0 0 hsa-miR-449b_st10 0 0 1 0 0 0 0 0 1 0 hsa-miR-487a_st2 0 0 0 1 0 0 1 0 0 0 0hsa-miR-494_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-miR-503_st1 0 0 0 1 0 0 0 0 00 0 hsa-miR-508_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-miR-509_st2 0 0 0 1 0 0 00 0 0 0 hsa-miR-513_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-miR-576_st2 0 0 0 0 00 0 1 0 0 0 hsa-miR-7_st1 0 0 0 0 0 0 1 0 0 0 0 hsa-asg-1020_st2 0 0 0 10 0 0 0 0 0 0 hsa-asg-10250_st1 0 0 0 0 0 0 0 1 0 0 0 hsa-asg-10304_st10 0 0 0 0 0 1 0 0 0 0 hsa-asg-1035_st2 0 0 0 0 0 0 0 0 0 0 0hsa-asg-10378_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-10478_st2 0 0 0 1 0 0 00 0 0 0 hsa-asg-1089_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-10947_st1 0 0 0 10 0 0 0 0 0 0 hsa-asg-11391_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-11425_st10 0 0 0 0 0 0 0 0 0 0 hsa-asg-11463_st1 0 0 1 0 0 0 0 0 0 0 0hsa-asg-11612_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-11777_st1 0 0 0 0 0 0 01 0 0 0 hsa-asg-1196_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-1199_st1 0 0 0 10 0 0 0 0 0 0 hsa-asg-1203_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-12142_st1 00 0 0 0 0 0 1 0 0 0 hsa-asg-12172_st2 1 0 0 0 0 0 0 0 0 0 0hsa-asg-12250_st2 0 0 0 0 0 0 1 0 0 0 0 hsa-asg-12253_st2 0 0 0 1 0 0 00 0 0 0 hsa-asg-12356_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-12440_st2 0 0 01 0 0 0 0 0 0 0 hsa-asg-124_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-12515_st20 0 0 1 0 0 0 0 0 0 0 hsa-asg-12531_st1 0 0 0 1 0 0 0 0 0 0 0hsa-asg-12552_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-1260_st1 0 1 0 0 0 0 0 00 0 0 hsa-asg-1270_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-12735_st1 0 0 0 1 00 0 0 0 0 0 hsa-asg-12799_st1 0 1 0 0 0 0 0 0 0 0 0 hsa-asg-12824_st1 00 0 0 0 0 1 0 0 0 0 hsa-asg-128_st2 0 0 0 0 0 0 0 1 0 0 0hsa-asg-12959_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-13077_st1 0 0 0 1 0 0 00 0 0 0 hsa-asg-13097_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-13159_st1 0 0 00 0 0 1 0 0 0 0 hsa-asg-13160_st1 0 0 0 1 0 0 0 0 0 0 0hsa-asg-13258_st2 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-13498_st1 0 0 0 0 0 0 00 0 0 1 hsa-asg-13589_st2 0 0 0 0 0 0 1 0 0 0 0 hsa-asg-13646_st1 0 0 00 0 0 0 0 0 0 0 hsa-asg-13741_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-1375_st11 0 0 0 0 0 0 0 0 0 0 hsa-asg-13801_st1 0 0 0 1 0 0 0 0 0 0 0hsa-asg-13878_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-13908_st2 0 0 0 0 0 0 00 0 0 0 hsa-asg-13997_st2 0 0 1 0 0 0 0 0 0 0 0 hsa-asg-14020_st2 0 0 00 0 0 1 0 0 0 0 hsa-asg-14134_st1 0 0 0 0 0 0 0 0 0 0 0hsa-asg-14147_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-14160_st1 0 0 0 0 0 0 00 0 0 0 hsa-asg-14176_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-14178_st2 0 0 00 0 0 1 0 0 0 0 hsa-asg-14209_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-1608_st20 0 0 1 0 0 0 0 0 0 0 hsa-asg-1619_st2 0 0 0 1 0 0 0 0 0 0 0hsa-asg-1765_st1 0 0 0 0 0 0 0 1 0 0 0 hsa-asg-1826_st2 0 0 0 0 0 0 0 10 0 0 hsa-asg-1881_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-2025_st2 0 0 0 0 00 0 0 0 0 0 hsa-asg-20_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-2303_st1 0 0 00 0 0 0 0 0 0 0 hsa-asg-2364_st1 0 0 0 0 0 0 1 0 0 0 0 hsa-asg-2381_st20 0 0 0 0 0 0 0 0 0 0 hsa-asg-2637_st1 0 0 0 0 0 0 1 0 0 0 0hsa-asg-2845_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-3009_st1 0 0 0 1 0 0 0 00 0 0 hsa-asg-3185_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-3322_st2 0 0 0 1 00 0 0 0 0 0 hsa-asg-3373_st2 0 0 0 0 0 0 1 0 0 0 0 hsa-asg-3376_st1 0 00 0 0 0 0 1 0 0 0 hsa-asg-3406_st1 0 0 0 1 0 0 0 0 0 0 0hsa-asg-3473_st1 1 0 0 0 0 0 0 0 0 0 0 hsa-asg-353_st1 0 0 0 0 0 0 0 0 00 0 hsa-asg-3595_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-3597_st2 0 0 0 1 0 00 0 0 0 0 hsa-asg-3672_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-3711_st2 0 0 00 0 0 0 0 1 0 0 hsa-asg-3731_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-3740_st10 0 0 1 0 0 0 0 0 0 0 hsa-asg-3742_st1 0 0 0 1 0 0 0 0 0 0 0hsa-asg-3743_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-3754_st2 0 0 0 1 0 0 0 00 0 0 hsa-asg-3782_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-3910_st2 0 0 0 0 00 0 0 0 0 0 hsa-asg-3922_st2 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-3979_st1 0 01 0 0 0 0 0 0 0 0 hsa-asg-3985_st1 0 0 0 0 0 0 1 0 0 0 0hsa-asg-4037_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-4201_st1 0 0 0 0 0 0 1 00 0 0 hsa-asg-4283_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-4441_st1 0 1 0 0 00 0 0 0 0 0 hsa-asg-4471_st1 0 0 0 0 0 0 1 0 0 0 0 hsa-asg-4472_st2 0 00 1 0 0 0 0 0 0 0 hsa-asg-4574_st2 0 0 0 0 0 0 1 0 0 0 0hsa-asg-4691_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-4756_st2 0 0 0 1 0 0 0 00 0 0 hsa-asg-4803_st2 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-4872_st1 0 0 0 1 00 0 0 0 0 0 hsa-asg-4913_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-5149_st2 0 00 1 0 0 0 0 0 0 0 hsa-asg-5205_st1 0 0 0 1 0 0 0 0 0 0 0hsa-asg-5455_st2 0 0 0 0 0 0 1 0 0 0 0 hsa-asg-558_st1 0 0 0 1 0 0 0 0 00 0 hsa-asg-5606_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-5618_st1 0 0 0 0 0 00 0 0 0 0 hsa-asg-562_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-5741_st2 0 0 0 10 0 0 0 0 0 0 hsa-asg-5886_st2 0 0 1 0 0 0 0 0 0 0 0 hsa-asg-5996_st2 00 0 1 0 0 0 0 0 0 0 hsa-asg-6027_st2 0 0 0 0 0 0 0 0 0 0 1hsa-asg-6160_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-6178_st2 1 0 0 0 0 0 0 00 0 0 hsa-asg-6377_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-6619_st1 0 0 0 1 00 0 0 0 0 0 hsa-asg-6623_st2 1 0 0 0 0 0 0 0 0 0 0 hsa-asg-6667_st1 0 00 0 0 0 1 0 0 0 0 hsa-asg-677_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-6815_st10 0 0 1 0 0 0 0 0 0 0 hsa-asg-6838_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-6845_st2 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-6880_st1 0 0 0 0 0 0 0 00 0 1 hsa-asg-6890_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-6915_st1 0 0 0 1 00 0 0 0 0 0 hsa-asg-7159_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-7388_st1 0 00 1 0 0 0 0 0 0 0 hsa-asg-7465_st2 0 0 0 0 0 0 1 0 0 0 0hsa-asg-7527_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-7615_st2 0 0 0 0 0 0 0 00 0 0 hsa-asg-7671_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-7751_st2 0 0 0 1 00 0 0 0 0 0 hsa-asg-7846_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-791_st1 0 0 00 0 0 0 1 0 0 0 hsa-asg-8039_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-8067_st20 0 0 0 0 1 0 0 0 0 0 hsa-asg-8129_st2 0 0 0 1 0 0 0 0 0 0 0hsa-asg-8138_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-8242_st2 0 0 0 1 0 0 0 00 0 0 hsa-asg-8293_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-8329_st1 0 0 0 1 00 0 0 0 0 0 hsa-asg-8330_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-8411_st2 0 00 0 0 0 0 0 0 0 0 hsa-asg-8486_st2 0 0 0 1 0 0 0 0 0 0 0hsa-asg-8630_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-8893_st1 0 0 0 1 0 0 0 00 0 0 hsa-asg-8987_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-9009_st1 0 0 0 1 00 0 0 0 0 0 hsa-asg-9303_st2 0 0 0 0 0 0 1 0 0 0 0 hsa-asg-9365_st2 0 00 0 0 0 1 0 0 0 0 hsa-asg-9491_st1 0 0 0 1 0 0 0 0 0 0 0hsa-asg-9510_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-asg-9512_st1 0 0 0 1 0 0 0 00 0 0 hsa-asg-9663_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-asg-9757_st1 0 0 0 0 00 1 0 0 0 0 hsa-asg-9939_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-9971_st2 0 00 0 0 0 1 0 0 0 0 hsa-cand330_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-cand443_st20 0 0 1 0 0 0 0 0 0 0 hsa-cand561_st1 0 0 0 1 0 0 0 0 0 0 0hsa-cand660_st1 0 0 0 0 0 0 1 0 0 0 0 hsa-cand733_st1 0 0 0 1 0 0 0 0 00 0 hsa-cand852_st2 0 0 0 0 0 0 0 0 0 0 0 hsa-cand91_st1 0 0 0 1 0 0 0 00 0 0 hsa-cand930_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-miR-101_st1 0 0 0 0 0 00 0 0 0 0 hsa-miR-122a_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-miR-128a_st2 0 0 00 0 0 1 0 0 0 0 hsa-miR-128b_st1 0 0 0 0 0 0 1 0 0 0 0 hsa-miR-129_st1 00 0 0 0 0 1 0 0 0 0 hsa-miR-135b_st2 0 0 0 1 0 0 0 0 0 0 0hsa-miR-137_st1 0 0 0 0 0 0 1 0 0 0 0 hsa-miR-138_st1 0 0 0 0 0 0 1 0 00 0 hsa-miR-153_st2 0 0 0 0 0 0 1 0 0 0 0 hsa-miR-193b_st1 0 0 0 0 0 0 00 0 0 0 hsa-miR-196b_st2 0 0 0 0 0 0 0 0 0 0 0 hsa-miR-199b_st2 0 0 0 00 0 0 0 0 0 0 hsa-miR-19a_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-miR-1_st1 0 0 00 0 0 0 0 0 0 0 hsa-miR-202_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-miR-212_st2 00 0 0 0 0 1 0 0 0 0 hsa-miR-216_st2 0 0 0 0 0 0 0 0 0 0 0hsa-miR-217_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-miR-218_st2 0 0 0 0 0 0 0 0 00 0 hsa-miR-224_st2 0 0 0 0 0 0 0 0 0 0 0 hsa-miR-299-3p_st2 0 0 0 1 0 00 0 0 0 0 hsa-miR-323_st1 0 0 0 0 0 0 1 0 0 0 0 hsa-miR-330_st2 0 0 0 00 0 1 0 0 0 0 hsa-miR-346_st1 0 0 0 0 0 0 1 0 0 0 0 hsa-miR-370_st1 0 00 0 0 0 1 0 0 0 0 hsa-miR-377_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-miR-452-_st10 0 0 0 0 0 0 0 0 0 0 hsa-miR-452_st1 0 0 0 0 0 0 0 0 0 0 0hsa-miR-455_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-miR-483_st1 0 0 0 0 0 0 0 0 00 0 hsa-miR-489_st2 0 1 0 0 0 0 0 0 0 0 0 hsa-miR-491_st1 0 0 0 0 0 0 10 0 0 0 hsa-miR-493-3p_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-miR-499_st1 0 0 0 00 0 0 0 0 0 0 hsa-miR-506_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-miR-510_st2 0 00 1 0 0 0 0 0 0 0 hsa-miR-514_st1 0 0 0 1 0 0 0 0 0 0 0hsa-miR-515-3p_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-miR-517a_st1 0 0 0 1 0 0 00 0 0 0 hsa-miR-517b_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-miR-517c_st2 0 0 0 00 0 0 1 0 0 0 hsa-miR-518f-_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-miR-519d_st1 00 0 1 0 0 0 0 0 0 0 hsa-miR-520a-_st2 0 0 0 1 0 0 0 0 0 0 0hsa-miR-520c_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-miR-520g_st1 0 0 0 1 0 0 0 00 0 0 hsa-miR-520h_st1 0 0 0 1 0 0 0 0 0 0 0 hsa-miR-525_st2 0 0 0 1 0 00 0 0 0 0 hsa-miR-526a_st2 0 0 0 1 0 0 0 0 0 0 0 hsa-miR-526b_st2 0 0 01 0 0 0 0 0 0 0 hsa-miR-526c_st1 0 0 0 1 0 0 0 0 0 0 0hsa-miR-542-3p_st1 0 0 0 0 0 1 0 0 0 0 0 hsa-miR-572_st1 0 0 0 1 0 0 0 00 0 0 hsa-miR-577_st1 0 0 0 0 0 0 1 0 0 0 0 hsa-miR-584_st2 0 0 0 0 0 01 0 0 0 0 hsa-miR-598_st2 0 0 0 0 0 0 1 0 0 0 0 hsa-miR-608_st2 0 0 0 10 0 0 0 0 0 0 hsa-miR140_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-miR141_st1 0 0 00 0 0 0 0 0 0 0 hsa-miR154_st1 0 0 0 0 0 0 0 0 0 0 0 hsa-miR157_st2 0 00 0 0 0 0 0 0 0 0 hsa-miR167_st2 0 0 0 0 0 0 0 0 0 0 0 hsa-miR177_st1 00 0 0 0 0 0 1 0 0 0 hsa-miR206_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-miR223_st20 0 0 1 0 0 0 0 0 0 0 Probe ID CRX CO BRS BLD ESO LIV OVR PRO LUN PANSUM hsa-miR-200a_st2 1 1 1 1 1 0 0 1 1 1 15 hsa-miR-200b_st1 1 1 1 1 1 00 1 1 1 15 hsa-miR-200c_st1 1 1 1 1 1 0 0 1 1 1 15 hsa-miR-429_st1 1 1 11 1 0 0 1 1 1 15 hsa-miR-605_st1 1 1 0 1 1 0 0 1 1 1 14 hsa-miR-187_st11 1 0 1 1 0 0 1 1 0 13 hsa-asg-7637_st2 1 1 0 0 1 0 0 1 1 1 12hsa-miR-203_st2 1 1 0 1 1 0 0 1 1 0 12 hsa-miR-375_st1 1 1 0 0 1 1 0 1 11 12 hsa-miR-411_st1 1 0 1 1 0 0 1 0 0 1 12 hsa-miR-487b_st2 1 0 1 1 0 01 1 0 1 12 hsa-asg-14153_st2 1 1 0 1 1 0 0 1 0 1 11 hsa-asg-5021_st1 1 01 1 0 0 1 1 0 1 11 hsa-miR-368_st2 1 0 1 1 0 0 1 0 0 1 11hsa-miR-493-5p_st1 1 0 0 1 0 0 1 1 0 1 11 hsa-miR-495_st1 1 0 0 1 0 0 11 0 0 11 hsa-miR-141_st1 0 1 0 0 0 0 0 1 1 1 10 hsa-miR-154_st2 1 0 0 10 0 1 0 0 1 10 hsa-miR-205_st1 1 0 1 1 1 0 0 1 1 0 10 hsa-miR-381_st2 10 0 1 0 0 1 0 0 1 10 hsa-asg-11715_st2 0 1 0 0 0 0 0 0 0 1 9hsa-asg-13254_st1 1 0 0 1 0 0 1 0 0 0 9 hsa-asg-13404_st2 1 0 0 0 0 0 10 0 1 9 hsa-asg-924_st1 0 1 0 1 1 0 0 0 0 0 9 hsa-asg-5617_st1 1 0 0 1 00 1 0 0 0 8 hsa-miR-539_st1 1 0 1 0 0 0 1 0 0 1 8 hsa-miR202_st2 1 0 0 00 0 1 0 0 1 8 hsa-asg-10049_st1 0 0 1 0 0 0 1 0 0 0 7 hsa-miR-127_st1 10 0 0 0 0 1 0 0 0 7 hsa-miR-133a_st1 1 0 0 1 1 0 0 1 0 0 7hsa-miR-133b_st2 1 1 0 1 1 0 0 1 0 0 7 hsa-miR-299-5p_st2 1 0 0 1 0 0 10 0 0 7 hsa-miR-432_st2 1 0 1 0 0 0 1 0 0 1 7 hsa-asg-10131_st2 0 1 0 00 0 0 0 1 1 6 hsa-asg-14182_st1 0 1 0 0 0 1 0 0 0 1 6 hsa-asg-7472_st2 00 0 0 0 0 1 0 0 1 6 hsa-cand206_st1 1 0 0 0 0 0 1 0 0 0 6hsa-miR-204_st2 1 0 0 0 0 0 1 0 0 0 6 hsa-miR-215_st1 0 1 0 0 0 1 0 0 01 6 hsa-miR-383_st1 0 0 0 0 0 0 1 0 0 0 6 hsa-miR-424_st1 1 0 0 0 0 0 10 0 0 6 hsa-asg-13166_st2 1 0 0 0 0 0 1 0 0 0 5 hsa-miR-134_st2 1 0 0 00 0 1 0 0 0 5 hsa-miR-192_st2 0 1 0 0 0 1 0 0 0 1 5 hsa-miR-194_st2 0 10 0 0 1 0 0 0 0 5 hsa-miR-206_st2 0 0 0 1 1 0 0 1 0 0 5 hsa-miR-338_st10 0 0 0 1 0 0 0 1 1 5 hsa-miR-379_st1 1 0 0 0 0 0 1 0 0 0 5hsa-miR-410_st1 0 0 0 0 0 0 1 0 0 1 5 hsa-miR-542-5p_st2 1 0 0 0 0 0 1 00 0 5 hsa-asg-12139_st2 0 0 0 0 0 0 0 0 0 1 4 hsa-asg-12227_st1 0 1 0 00 0 0 0 0 0 4 hsa-asg-13705_st1 0 0 0 0 0 0 0 0 0 1 4 hsa-asg-2027_st1 00 0 1 1 0 0 0 0 0 4 hsa-asg-2345_st2 0 0 0 1 1 0 0 0 0 0 4hsa-asg-8428_st2 0 0 0 0 1 0 0 0 1 0 4 hsa-miR-184_st1 0 0 0 0 0 0 0 0 10 4 hsa-miR-189_st1 1 0 0 1 1 0 0 0 0 0 4 hsa-miR-200a-_st2 0 1 0 0 0 00 0 0 0 4 hsa-miR-34c_st2 0 0 0 0 0 0 0 0 1 0 4 hsa-miR-376a_st2 1 0 0 10 0 1 0 0 0 4 hsa-miR-376b_st1 0 0 0 0 0 0 1 0 0 0 4 hsa-miR-382_st1 1 00 0 0 0 1 0 0 0 4 hsa-miR-409-3p_st1 1 0 0 0 0 0 0 0 0 0 4hsa-miR-485-5p_st2 1 0 0 0 0 0 1 0 0 0 4 hsa-miR-592_st1 0 0 0 1 0 0 1 00 0 4 hsa-miR-96_st1 0 0 0 0 0 0 0 1 0 1 4 hsa-miR-9_st2 1 0 0 0 0 0 0 00 0 4 hsa-miR23_st2 0 0 0 0 0 0 1 0 0 0 4 hsa-asg-1088_st1 0 0 0 0 0 0 00 0 0 3 hsa-asg-11443_st1 0 0 0 0 0 0 1 0 0 0 3 hsa-asg-1296_st1 0 0 0 00 0 0 0 0 1 3 hsa-asg-14208_st1 0 0 0 0 0 1 0 0 0 0 3 hsa-asg-14228_st20 0 0 0 0 0 1 0 0 0 3 hsa-asg-1521_st1 0 0 0 0 0 0 0 0 0 0 3hsa-asg-1794_st1 0 0 0 0 1 0 0 0 1 0 3 hsa-asg-4915_st1 0 0 0 0 0 0 0 10 0 3 hsa-asg-4978_st2 0 0 0 0 0 0 0 0 0 0 3 hsa-asg-5119_st1 0 0 0 0 00 0 0 0 1 3 hsa-asg-5638_st2 0 0 0 0 0 0 1 0 0 0 3 hsa-asg-8128_st1 0 00 0 0 1 0 0 0 1 3 hsa-asg-9696_st1 1 0 0 0 0 0 1 0 0 0 3 hsa-cand150_st20 0 0 0 0 0 0 0 1 0 3 hsa-miR-132_st1 1 0 0 0 0 0 0 0 0 0 3hsa-miR-149_st2 0 0 0 0 0 0 0 1 0 0 3 hsa-miR-31_st2 0 0 0 1 0 0 0 0 0 03 hsa-miR-369-5p_st2 1 0 0 0 0 0 1 0 0 0 3 hsa-miR-433_st2 0 0 0 0 0 0 10 0 0 3 hsa-miR-449_st2 0 0 0 0 0 0 0 0 1 0 3 hsa-miR-450_st2 1 0 0 0 00 0 0 0 0 3 hsa-miR-582_st1 0 0 0 1 0 0 0 0 0 0 3 hsa-miR-9-_st2 1 0 0 00 0 0 0 0 0 3 hsa-miR12_st1 0 0 0 0 1 0 0 0 0 0 3 hsa-miR144_st2 0 0 0 00 0 1 0 0 0 3 hsa-asg-10080_st1 0 0 0 0 0 0 0 0 0 1 2 hsa-asg-10202_st20 0 0 1 0 0 0 0 0 0 2 hsa-asg-10247_st1 0 0 0 0 0 0 0 0 0 1 2hsa-asg-10309_st1 0 0 0 0 0 0 0 0 0 0 2 hsa-asg-10393_st1 0 0 0 0 0 0 00 0 0 2 hsa-asg-10881_st1 0 0 0 0 0 0 0 0 0 0 2 hsa-asg-10883_st1 1 0 00 0 0 1 0 0 0 2 hsa-asg-11088_st1 0 0 0 0 0 0 0 0 0 1 2hsa-asg-11338_st2 0 0 0 0 0 0 1 0 0 0 2 hsa-asg-13135_st1 0 0 0 0 0 0 00 0 0 2 hsa-asg-13231_st1 0 0 0 0 0 0 0 0 1 0 2 hsa-asg-13284_st1 0 0 01 0 0 0 0 0 0 2 hsa-asg-13922_st1 0 0 1 0 0 0 0 0 0 0 2hsa-asg-14065_st2 0 0 0 0 0 0 0 0 0 0 2 hsa-asg-2399_st2 0 0 0 0 0 0 0 00 0 2 hsa-asg-2424_st1 0 0 0 0 0 0 0 0 0 0 2 hsa-asg-2484_st2 0 0 0 0 00 0 0 0 0 2 hsa-asg-3081_st1 0 0 0 0 0 0 0 0 0 0 2 hsa-asg-3822_st2 0 00 0 0 0 0 0 0 1 2 hsa-asg-3983_st1 0 0 1 0 0 0 0 0 0 0 2hsa-asg-4209_st2 0 0 1 0 0 0 0 0 0 0 2 hsa-asg-4219_st1 0 0 0 0 0 0 0 00 0 2 hsa-asg-4438_st2 0 0 0 0 0 0 0 0 1 0 2 hsa-asg-4439_st2 0 0 0 0 00 0 0 1 0 2 hsa-asg-5602_st2 0 0 0 1 1 0 0 0 0 0 2 hsa-asg-5630_st2 0 00 0 0 0 1 0 0 0 2 hsa-asg-6283_st1 0 0 0 0 0 0 0 0 0 0 2hsa-asg-7295_st2 0 0 0 0 0 0 0 0 0 0 2 hsa-asg-8480_st1 0 0 0 0 0 0 0 00 0 2 hsa-asg-8616_st2 0 0 0 0 0 0 0 0 0 1 2 hsa-asg-9198_st1 0 0 0 0 10 0 0 0 0 2 hsa-asg-9219_st2 0 0 0 0 0 0 0 0 0 1 2 hsa-asg-9262_st1 0 00 0 0 0 0 0 0 0 2 hsa-asg-9269_st2 0 0 0 0 0 0 0 0 1 0 2hsa-asg-9514_st1 0 0 0 0 0 0 0 0 0 0 2 hsa-asg-9687_st1 1 0 0 0 0 0 1 00 0 2 hsa-asg-9830_st2 0 0 0 0 0 0 0 0 0 1 2 hsa-cand14_st1 0 0 0 0 0 00 0 0 0 2 hsa-cand26_st2 0 0 0 0 0 0 0 0 0 0 2 hsa-cand317_st1 0 0 0 0 00 0 0 0 0 2 hsa-cand79_st1 0 0 0 0 0 0 0 0 0 0 2 hsa-miR-105_st1 0 0 0 00 0 0 0 0 0 2 hsa-miR-139_st2 0 0 0 0 0 0 0 0 0 0 2 hsa-miR-154-_st2 0 00 0 0 0 0 0 0 0 2 hsa-miR-182_st1 0 0 0 0 0 0 0 0 0 1 2 hsa-miR-183_st10 0 0 0 0 0 0 0 0 1 2 hsa-miR-328_st1 0 0 0 0 0 0 1 0 0 0 2hsa-miR-329_st1 1 0 0 0 0 0 0 0 0 0 2 hsa-miR-378_st1 0 0 0 1 0 0 0 0 00 2 hsa-miR-380-3p_st2 0 0 0 0 0 0 0 0 0 0 2 hsa-miR-409-5p_st1 0 0 0 00 0 0 0 0 0 2 hsa-miR-449b_st1 0 0 0 0 0 0 0 0 0 0 2 hsa-miR-487a_st2 00 0 0 0 0 0 0 0 0 2 hsa-miR-494_st1 1 0 0 0 0 0 0 0 0 0 2hsa-miR-503_st1 1 0 0 0 0 0 0 0 0 0 2 hsa-miR-508_st1 0 0 0 0 0 0 1 0 00 2 hsa-miR-509_st2 0 0 0 0 0 0 1 0 0 0 2 hsa-miR-513_st1 0 0 0 0 0 0 10 0 0 2 hsa-miR-576_st2 0 0 0 0 1 0 0 0 0 0 2 hsa-miR-7_st1 0 0 0 0 0 00 0 0 1 2 hsa-asg-1020_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-10250_st1 0 0 00 0 0 0 0 0 0 1 hsa-asg-10304_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-1035_st20 0 0 0 0 1 0 0 0 0 1 hsa-asg-10378_st1 0 0 0 0 0 0 1 0 0 0 1hsa-asg-10478_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-1089_st1 0 0 0 0 0 0 0 00 0 1 hsa-asg-10947_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-11391_st1 0 0 0 00 1 0 0 0 0 1 hsa-asg-11425_st1 0 0 0 0 0 1 0 0 0 0 1 hsa-asg-11463_st10 0 0 0 0 0 0 0 0 0 1 hsa-asg-11612_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-11777_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-1196_st1 0 0 0 0 0 0 0 00 0 1 hsa-asg-1199_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-1203_st1 0 0 0 0 00 0 0 0 0 1 hsa-asg-12142_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-12172_st2 00 0 0 0 0 0 0 0 0 1 hsa-asg-12250_st2 0 0 0 0 0 0 0 0 0 0 1hsa-asg-12253_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-12356_st1 0 0 0 0 0 0 00 0 0 1 hsa-asg-12440_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-124_st1 0 0 0 00 0 0 0 0 0 1 hsa-asg-12515_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-12531_st10 0 0 0 0 0 0 0 0 0 1 hsa-asg-12552_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-1260_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-1270_st2 0 0 0 0 0 0 0 00 0 1 hsa-asg-12735_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-12799_st1 0 0 0 00 0 0 0 0 0 1 hsa-asg-12824_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-128_st2 00 0 0 0 0 0 0 0 0 1 hsa-asg-12959_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-13077_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-13097_st1 1 0 0 0 0 0 00 0 0 1 hsa-asg-13159_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-13160_st1 0 0 00 0 0 0 0 0 0 1 hsa-asg-13258_st2 0 0 0 0 0 0 1 0 0 0 1hsa-asg-13498_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-13589_st2 0 0 0 0 0 0 00 0 0 1 hsa-asg-13646_st1 0 0 0 0 0 0 1 0 0 0 1 hsa-asg-13741_st2 0 0 00 0 0 0 0 0 0 1 hsa-asg-1375_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-13801_st10 0 0 0 0 0 0 0 0 0 1 hsa-asg-13878_st2 0 0 0 0 0 0 0 0 0 0 1hsa-asg-13908_st2 0 0 0 0 0 0 0 0 0 1 1 hsa-asg-13997_st2 0 0 0 0 0 0 00 0 0 1 hsa-asg-14020_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-14134_st1 1 0 00 0 0 0 0 0 0 1 hsa-asg-14147_st1 0 0 0 0 0 0 1 0 0 0 1hsa-asg-14160_st1 0 0 1 0 0 0 0 0 0 0 1 hsa-asg-14176_st1 1 0 0 0 0 0 00 0 0 1 hsa-asg-14178_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-14209_st1 0 0 00 0 0 0 0 0 0 1 hsa-asg-1608_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-1619_st20 0 0 0 0 0 0 0 0 0 1 hsa-asg-1765_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-1826_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-1881_st2 0 0 0 0 0 0 0 00 0 1 hsa-asg-2025_st2 0 0 0 0 0 0 1 0 0 0 1 hsa-asg-20_st1 0 0 0 0 0 00 0 0 0 1 hsa-asg-2303_st1 0 0 0 0 0 1 0 0 0 0 1 hsa-asg-2364_st1 0 0 00 0 0 0 0 0 0 1 hsa-asg-2381_st2 0 0 0 0 0 0 0 0 0 1 1 hsa-asg-2637_st10 0 0 0 0 0 0 0 0 0 1 hsa-asg-2845_st2 0 0 0 0 0 0 0 0 0 0 1hsa-asg-3009_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-3185_st1 0 0 0 0 0 0 0 00 0 1 hsa-asg-3322_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-3373_st2 0 0 0 0 00 0 0 0 0 1 hsa-asg-3376_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-3406_st1 0 00 0 0 0 0 0 0 0 1 hsa-asg-3473_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-353_st10 0 0 0 0 0 0 0 0 1 1 hsa-asg-3595_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-3597_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-3672_st1 0 0 0 0 0 0 0 00 0 1 hsa-asg-3711_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-3731_st1 0 0 0 0 00 0 0 0 0 1 hsa-asg-3740_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-3742_st1 0 00 0 0 0 0 0 0 0 1 hsa-asg-3743_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-3754_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-3782_st1 0 0 0 0 0 0 0 00 0 1 hsa-asg-3910_st2 0 0 0 1 0 0 0 0 0 0 1 hsa-asg-3922_st2 0 0 0 0 01 0 0 0 0 1 hsa-asg-3979_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-3985_st1 0 00 0 0 0 0 0 0 0 1 hsa-asg-4037_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-4201_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-4283_st2 0 0 0 0 0 0 0 00 0 1 hsa-asg-4441_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-4471_st1 0 0 0 0 00 0 0 0 0 1 hsa-asg-4472_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-4574_st2 0 00 0 0 0 0 0 0 0 1 hsa-asg-4691_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-4756_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-4803_st2 0 0 1 0 0 0 0 00 0 1 hsa-asg-4872_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-4913_st1 0 0 0 0 00 0 0 0 1 1 hsa-asg-5149_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-5205_st1 0 00 0 0 0 0 0 0 0 1 hsa-asg-5455_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-558_st10 0 0 0 0 0 0 0 0 0 1 hsa-asg-5606_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-5618_st1 0 0 0 0 0 0 0 0 0 1 1 hsa-asg-562_st1 1 0 0 0 0 0 0 0 00 1 hsa-asg-5741_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-5886_st2 0 0 0 0 0 00 0 0 0 1 hsa-asg-5996_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-6027_st2 0 0 00 0 0 0 0 0 0 1 hsa-asg-6160_st1 0 0 0 0 0 0 0 0 0 1 1 hsa-asg-6178_st20 0 0 0 0 0 0 0 0 0 1 hsa-asg-6377_st2 0 0 0 0 0 0 0 0 0 0 1hsa-asg-6619_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-6623_st2 0 0 0 0 0 0 0 00 0 1 hsa-asg-6667_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-677_st2 0 0 0 0 0 00 0 0 0 1 hsa-asg-6815_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-6838_st1 0 0 00 0 0 0 0 0 0 1 hsa-asg-6845_st2 0 0 0 1 0 0 0 0 0 0 1 hsa-asg-6880_st10 0 0 0 0 0 0 0 0 0 1 hsa-asg-6890_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-6915_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-7159_st2 0 0 0 0 0 0 0 00 0 1 hsa-asg-7388_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-7465_st2 0 0 0 0 00 0 0 0 0 1 hsa-asg-7527_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-7615_st2 0 00 0 0 0 0 0 0 1 1 hsa-asg-7671_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-7751_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-7846_st1 0 0 0 0 0 0 0 00 0 1 hsa-asg-791_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-8039_st2 0 0 0 0 0 00 0 0 0 1 hsa-asg-8067_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-8129_st2 0 0 00 0 0 0 0 0 0 1 hsa-asg-8138_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-8242_st20 0 0 0 0 0 0 0 0 0 1 hsa-asg-8293_st2 0 0 0 0 0 0 0 0 0 0 1hsa-asg-8329_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-8330_st2 0 0 0 0 0 0 0 00 0 1 hsa-asg-8411_st2 0 0 0 0 0 0 1 0 0 0 1 hsa-asg-8486_st2 0 0 0 0 00 0 0 0 0 1 hsa-asg-8630_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-8893_st1 0 00 0 0 0 0 0 0 0 1 hsa-asg-8987_st1 0 0 0 0 0 0 0 0 0 0 1hsa-asg-9009_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-9303_st2 0 0 0 0 0 0 0 00 0 1 hsa-asg-9365_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-9491_st1 0 0 0 0 00 0 0 0 0 1 hsa-asg-9510_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-9512_st1 0 00 0 0 0 0 0 0 0 1 hsa-asg-9663_st1 0 0 0 0 0 1 0 0 0 0 1hsa-asg-9757_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-asg-9939_st2 0 0 0 0 0 0 0 00 0 1 hsa-asg-9971_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-cand330_st2 0 0 0 0 0 00 0 0 0 1 hsa-cand443_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-cand561_st1 0 0 0 00 0 0 0 0 0 1 hsa-cand660_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-cand733_st1 0 00 0 0 0 0 0 0 0 1 hsa-cand852_st2 0 0 0 0 0 0 0 0 0 1 1 hsa-cand91_st1 00 0 0 0 0 0 0 0 0 1 hsa-cand930_st1 0 0 0 0 0 0 0 0 0 0 1hsa-miR-101_st1 0 0 0 0 0 0 1 0 0 0 1 hsa-miR-122a_st1 0 0 0 0 0 1 0 0 00 1 hsa-miR-128a_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-128b_st1 0 0 0 0 0 00 0 0 0 1 hsa-miR-129_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-135b_st2 0 0 0 00 0 0 0 0 0 1 hsa-miR-137_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-138_st1 0 00 0 0 0 0 0 0 0 1 hsa-miR-153_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-193b_st11 0 0 0 0 0 0 0 0 0 1 hsa-miR-196b_st2 1 0 0 0 0 0 0 0 0 0 1hsa-miR-199b_st2 1 0 0 0 0 0 0 0 0 0 1 hsa-miR-19a_st1 0 0 0 0 0 0 1 0 00 1 hsa-miR-1_st1 1 0 0 0 0 0 0 0 0 0 1 hsa-miR-202_st1 0 0 0 0 0 0 0 00 0 1 hsa-miR-212_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-216_st2 0 0 0 0 0 00 0 0 1 1 hsa-miR-217_st1 0 0 0 0 0 0 0 0 0 1 1 hsa-miR-218_st2 1 0 0 00 0 0 0 0 0 1 hsa-miR-224_st2 1 0 0 0 0 0 0 0 0 0 1 hsa-miR-299-3p_st2 00 0 0 0 0 0 0 0 0 1 hsa-miR-323_st1 0 0 0 0 0 0 0 0 0 0 1hsa-miR-330_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-346_st1 0 0 0 0 0 0 0 0 00 1 hsa-miR-370_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-377_st1 0 0 0 0 0 0 00 0 0 1 hsa-miR-452-_st1 0 0 1 0 0 0 0 0 0 0 1 hsa-miR-452_st1 1 0 0 0 00 0 0 0 0 1 hsa-miR-455_st1 1 0 0 0 0 0 0 0 0 0 1 hsa-miR-483_st1 0 0 00 0 1 0 0 0 0 1 hsa-miR-489_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-491_st1 00 0 0 0 0 0 0 0 0 1 hsa-miR-493-3p_st1 0 0 0 0 0 0 1 0 0 0 1hsa-miR-499_st1 0 0 0 0 1 0 0 0 0 0 1 hsa-miR-506_st2 0 0 0 0 0 0 0 0 00 1 hsa-miR-510_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-514_st1 0 0 0 0 0 0 00 0 0 1 hsa-miR-515-3p_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-517a_st1 0 0 00 0 0 0 0 0 0 1 hsa-miR-517b_st1 0 0 1 0 0 0 0 0 0 0 1 hsa-miR-517c_st20 0 0 0 0 0 0 0 0 0 1 hsa-miR-518f-_st2 0 0 0 0 0 0 0 0 0 0 1hsa-miR-519d_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-520a-_st2 0 0 0 0 0 0 0 00 0 1 hsa-miR-520c_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-520g_st1 0 0 0 0 00 0 0 0 0 1 hsa-miR-520h_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-525_st2 0 0 00 0 0 0 0 0 0 1 hsa-miR-526a_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-526b_st20 0 0 0 0 0 0 0 0 0 1 hsa-miR-526c_st1 0 0 0 0 0 0 0 0 0 0 1hsa-miR-542-3p_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-572_st1 0 0 0 0 0 0 0 00 0 1 hsa-miR-577_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-584_st2 0 0 0 0 0 00 0 0 0 1 hsa-miR-598_st2 0 0 0 0 0 0 0 0 0 0 1 hsa-miR-608_st2 0 0 0 00 0 0 0 0 0 1 hsa-miR140_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-miR141_st1 0 0 00 0 0 1 0 0 0 1 hsa-miR154_st1 0 0 1 0 0 0 0 0 0 0 1 hsa-miR157_st2 1 00 0 0 0 0 0 0 0 1 hsa-miR167_st2 0 0 1 0 0 0 0 0 0 0 1 hsa-miR177_st1 00 0 0 0 0 0 0 0 0 1 hsa-miR206_st1 0 0 0 0 0 0 0 0 0 0 1 hsa-miR223_st20 0 0 0 0 0 0 0 0 0 1 Abbreviations: THY, thymus; KID, kideny; STO,stomach; TES, testicle; UTR, uterus; SPL, spleen; BRN, brain; TNG,tongue; TMS, thymus; TRA, trachea; SI, small intestine; CRX, cervix;COL, colon; BRS, breast; BLD, bladder; ESO, esophagus; LIV, liver; OVR,ovary; PRO, prostate; LUN, lung; PAN, pancreas

Example 39 miRNA Combinations for the Detection of Lymph Node Metastasesfrom Different Cancer Types

The inventors evaluated the most differentially-expressed miRNA markersbetween positive and negative lymph nodes from patients with cervicalcancer (Example 3), colon cancer (Example 17) and breast cancer (Example40) and determined that several useful markers (miR-141, -203, miR-429,miR-200a, miR-200b, and miR-200c ) are conserved for these differentcancer types. qRT-PCR quantification of these miRNAs, from positive andnegative lymph nodes from patients with cervical cancer and coloncancer, was performed as described above in Example 7, Example 17, andExample 40) (FIG. 12A). As shown in FIG. 12B, the summation of thenormalized Ct values for representative combinations of these miRNAsincreased the separation between the positive and negative lymph nodesin each cancer type. More generally, common miRNA markers useful for thedetermination of metastasis of multiple cancers can be identified aftercomparison of the different normal tissues and normal lymph nodes, asreported in Example 38 (Table 37).

Example 40 miRNAs Differentially Expressed Between PairedCancer-Positive and Cancer-Negative Axillary Lymph Nodes from BreastCancer Patients

To identify miRNAs that may be useful markers for the presence of breastcancer metastasis in axillary lymph nodes, the inventors identifiedmiRNAs that are expressed at significantly higher levels incancer-positive lymph nodes (LNpos) compared to cancer-negative lymphnodes (LNneg) from breast cancer patients.

FFPE samples of paired cancer-positive (LNpos 1-4) and cancer-negative(LNneg 1-4) axillary lymph nodes from ductal breast cancer patients werepurchased from ProteoGenex (ProteoGenex, Inc.; Culver City, Calif., USA)(Table 38). A pathology report and hematoxilin-eosin (H&E)-stainedslides were available for each lymph node sample. H&E slides werereviewed by a pathologist at Asuragen to confirm lymph node statuses ascancer cell-positive or cancer cell-negative. Positive lymph node 1(LNpos1) contained only a minute amount of tumor cells and was excludedfrom the analysis. Its counterpart sample, LNneg1, was kept in theanalysis, unmatched.

TABLE 38 Patient and tissue sample pathology information for four,paired cancer-positive (LNpos) and cancer-negative (LNneg) axillarylymph nodes from four independent ductal breast cancer patients. LNPatient Patient's Tumor Sample Cancer Age (yr) Histological size TNMAJCC LN ID Diagnosis Sex Ethnicity Diagnosis (cm) Stage Stage MetsLNneg1 normal F 72 infiltrating ductal 2.5 T2N1M0 IIB 1/11 LNpos1metastatic Caucasian carcinoma, moderately differentiated LNneg2 normalF 46 infiltrating ductal 2.5 T2N2M0 IIIA 5/9  LNpos2 metastaticCaucasian and lobular carcinoma, moderately differentiated LNneg3 normalF 66 infiltrating ductal 3.5 T2N1M0 IIB 1/11 LNpos3 metastatic Caucasiancarcinoma, moderately differentiated LNneg4 normal F 65 infiltratingductal 3 T2N1M0 IIB 1/8  LNpos4 metastatic Caucasian carcinoma,moderately differentiated TNM stage (Sobin and Wittekind, 2002); AJCCStage (Greene, 2002); LN Mets, number of lymph nodes withmetastases/total number of lymph nodes examined.

Total RNA isolation from the FFPE lymph node samples was performed usingthe RecoverAll™ Total Nucleic Acid Isolation Kit (cat. No. AM1975;Ambion, Inc.; Austin, Tex., USA), as described above in Example 1 andanalyzed on miRNA microarrays, as described below.

miRNA expression was compared between three paired LN samples from threeindividual patients (LNpos2-4; LNneg2-4) plus one unmatchedcancer-negative lymph node (LNneg1). Total RNA from each sample (200 ng)was processed by Asuragen Services (Asuragen Inc., Austin, Tex., USA),according to the company's standard operating procedures. RNA labelingwas adapted from a published method (Wang et. al., 2006) that wasmodified so that RNA is end-labeled with biotin instead of Cy3. Briefly,total RNA is dephosphorylated with calf intestinal phosphatase and thepCp-Biotin labeling molecule is ligated to the 3′ ends of the RNAmolecules. Labeled RNA was purified using Bio-Spin 6 columns (Bio-RadLaboratories, Inc.; Hercules, Calif., USA). Labeled RNA was hybridizedto a custom-made microRNA array (miRChip V1.0, manufactured byAffymetrix, Santa Clara, Calif., USA for Ambion), as described above inExample 2. Hybridization, washing, staining, imaging, and signalextraction were performed as recommended by the miRChip manufacturer,except that the 20× GeneChip® Eukaryotic Hybridization Control cocktailwas omitted. Signal processing was as described above in Example 2.

A total of 146 miRNAs (50 verified and 96 predicted) exhibitedsignificant differential expression between positive (LNpos) andnegative (LNneg) lymph node samples (t-test (LNpos vs LNneg)<0.05 andlog 2-Diff (LNpos vs LNneg)≧1; Flag=1) (Table 39). Among the verifiedmiRNAs, four (hsa-miR-375, -200b, -141, and -200a) had averageexpression levels between 100- and 350-fold higher in LNpos compared toLNneg; eight (hsa-miR-182, -196a, -200c, -203, -183, -429, -542-5p, and-421-3p) had average expression levels between 10- and 90-fold higher inLNpos; and nine had average expression levels between 5- and 10-foldhigher in LNpos samples than in LNneg samples (Table 39). Among thepredicted miRNAs, two (hsa-asg-2924_st1 and -1937_st1) had averageexpression levels at least 10-fold higher in LNpos and thirteen hadaverage expression levels between 5- and 9-fold higher in LNpos than inLNneg samples. In addition, four miRNAs (hsa-asg-6116_st2, -6449_st1,-10487_st1, and -2449_st1) had average expression levels between 5- and20 fold lower in LNpos compared to LNneg (Table 39).

TABLE 39 miRNAs differentially expressed between three cancer-positive(LNpos2-4) and four cancer-negative (LNneg1-4) lymph node samples frombreast cancer patients. Probe LNneg MicroRNA ID 1 2 3 4 Mean SD %hsa-let-7e hsa-let-7e_st1 10.2 9.61 10.1 10.4 10.0 0.34 100 hsa-miR-103hsa-miR-103_st1 10.7 10.7 11.2 11.2 11.0 0.26 100 hsa-miR-106bhsa-miR-106b_st2 7.39 6.69 8.17 7.51 7.44 0.61 100 hsa-miR-107hsa-miR-107_st2 10.2 10.5 10.9 11.0 10.7 0.34 100 hsa-miR-128ahsa-miR-128a_st2 5.28 5.95 6.91 6.64 6.19 0.73 100 hsa-miR-130bhsa-miR-130b_st1 5.88 6.53 5.93 6.29 6.16 0.31 75 hsa-miR-141hsa-miR-141_st1 1.83 1.74 1.19 2.79 1.89 0.67 0 hsa-miR-145hsa-miR-145_st1 12.2 12.2 12.6 12.7 12.4 0.23 100 hsa-miR-148ahsa-miR-148a_st1 8.68 7.61 8.84 8.70 8.46 0.57 100 hsa-miR-148bhsa-miR-148b_st2 4.91 4.53 5.26 6.53 5.31 0.87 100 hsa-miR-151hsa-miR-151_st2 6.77 6.09 6.13 7.08 6.52 0.48 100 hsa-miR-181ahsa-miR-181a_st1 9.13 9.33 10.1 9.38 9.49 0.43 100 hsa-miR-181a*hsa-miR-213_st1 2.51 5.30 1.53 4.85 3.55 1.82 0 hsa-miR-181dhsa-miR-181d_st1 4.18 4.73 4.23 3.75 4.22 0.40 25 hsa-miR-182hsa-miR-182_st1 4.12 3.75 4.13 2.79 3.70 0.63 25 hsa-miR-183hsa-miR-183_st1 1.22 2.61 1.98 3.34 2.29 0.91 0 hsa-miR-189hsa-miR-189_st1 1.54 0.58 0.61 1.94 1.17 0.68 0 hsa-miR-191hsa-miR-191_st2 11.1 11.0 11.6 11.6 11.3 0.32 100 hsa-miR-193bhsa-miR-193b_st1 8.14 7.05 8.93 8.20 8.08 0.77 25 hsa-miR-196ahsa-miR-196a_st2 1.83 1.35 2.90 5.38 2.86 1.80 25 hsa-miR-199ahsa-miR-199a_st2 9.70 9.17 9.48 9.31 9.42 0.23 100 hsa-miR-200ahsa-miR-200a_st2 2.08 2.45 2.55 2.79 2.47 0.29 0 hsa-miR-200a*hsa-miR-200a-_st2 4.36 3.93 2.55 2.92 3.44 0.85 0 hsa-miR-200bhsa-miR-200b_st1 2.51 1.86 2.55 3.52 2.61 0.68 0 hsa-miR-200chsa-miR-200c_st1 5.06 5.86 5.74 7.26 5.98 0.92 100 hsa-miR-203hsa-miR-203_st2 3.36 3.34 2.45 2.65 2.95 0.47 0 hsa-miR-21hsa-miR-21_st2 10.8 9.74 10.5 10.9 10.4 0.53 100 hsa-miR-218hsa-miR-218_st2 4.05 1.35 2.90 1.94 2.56 1.18 25 hsa-miR-22hsa-miR-22_st1 8.89 9.06 9.09 9.27 9.08 0.16 100 hsa-miR-224hsa-miR-224_st2 5.45 5.33 4.29 6.19 5.32 0.78 75 hsa-miR-24hsa-miR-24_st1 10.2 10.1 10.8 10.9 10.5 0.43 100 hsa-miR-25hsa-miR-25_st2 9.69 9.79 10.0 9.77 9.82 0.15 100 hsa-miR-27ahsa-miR-27a_st2 8.41 8.64 8.45 9.08 8.65 0.30 100 hsa-miR-30a-3phsa-miR-30a-3p_st1 4.87 5.94 5.55 6.75 5.78 0.78 75 hsa-miR-30a-5phsa-miR-30a-5p_st2 9.61 9.62 9.73 10.0 9.76 0.22 100 hsa-miR-30e-5phsa-miR-30e-5p_st2 8.61 7.64 8.19 8.22 8.17 0.40 100 hsa-miR-34ahsa-miR-34a_st1 5.90 5.96 6.98 7.40 6.56 0.75 100 hsa-miR-34bhsa-miR-34b_st2 2.69 2.08 2.34 2.65 2.44 0.29 0 hsa-miR-363hsa-miR-363_st2 6.24 7.00 6.45 7.05 6.68 0.40 100 hsa-miR-373*hsa-miR-373-_st2 7.87 7.95 7.54 7.55 7.73 0.21 100 hsa-miR-375hsa-miR-375_st1 1.38 1.48 0.23 5.42 2.13 2.27 0 hsa-miR-376ahsa-asg-14234_st1 1.83 1.62 2.11 1.46 1.75 0.28 0 hsa-miR-421hsa-miR-421-3p_st1 3.13 1.86 2.82 1.71 2.38 0.70 0 hsa-miR-425-5phsa-asg-14166_st2 8.42 8.16 8.63 8.23 8.36 0.21 100 hsa-miR-429hsa-miR-429_st1 1.83 3.60 1.84 3.82 2.77 1.09 0 hsa-miR-452hsa-miR-452_st1 4.18 5.62 5.35 4.67 4.96 0.65 50 hsa-miR-489hsa-miR-489_st2 3.13 1.62 1.98 2.79 2.38 0.70 0 hsa-miR-508hsa-miR-508_st1 4.12 3.13 3.82 3.25 3.58 0.47 25 hsa-miR-539hsa-miR-539_st1 2.08 2.28 2.34 1.94 2.16 0.18 0 hsa-miR-542-5phsa-miR-542-5p_st2 1.83 2.69 0.81 0.89 1.55 0.89 0 hsa-miR-654hsa-miR-654_st1 8.29 7.27 6.92 7.62 7.53 0.58 50 hsa-miR-93hsa-miR-93_st2 7.78 8.07 7.65 7.70 7.80 0.19 100 hsa-miR-98hsa-miR-98_st1 6.21 5.76 6.81 6.05 6.21 0.44 100 hsa-miR-99bhsa-miR-99b_st1 8.04 8.22 8.49 8.50 8.31 0.22 0 hsa-asg-9769hsa-asg-9769_st2 2.99 2.45 2.23 3.25 2.73 0.47 0 hsa-asg-8901hsa-asg-8901_st2 12.3 12.2 12.2 12.1 12.2 0.06 100 hsa-asg-860hsa-asg-860_st2 2.99 2.61 2.34 2.65 2.65 0.27 0 hsa-asg-6457hsa-asg-6457_st2 3.36 3.43 3.59 2.92 3.32 0.29 0 hsa-asg-5021hsa-asg-5021_st1 2.08 2.61 1.98 1.46 2.03 0.47 0 hsa-asg-14175hsa-asg-14175_st1 2.08 2.45 2.34 2.65 2.38 0.24 0 hsa-asg-13258hsa-asg-13258_st2 4.52 5.79 5.44 6.49 5.56 0.82 25 hsa-asg-13254hsa-asg-13254_st1 1.22 1.74 3.05 4.28 2.57 1.38 0 hsa-asg-13205hsa-asg-13205_st1 2.31 4.05 3.59 3.34 3.32 0.74 0 hsa-asg-12885hsa-asg-12885_st1 3.13 2.76 2.90 2.79 2.89 0.17 0 hsa-asg-12471hsa-asg-12471_st1 2.31 1.86 1.84 2.15 2.04 0.23 0 hsa-asg-12372hsa-asg-12372_st2 10.4 10.3 10.3 10.1 10.3 0.10 100 hsa-asg-11896hsa-asg-11896_st2 11.6 11.9 11.9 11.9 11.8 0.15 25 hsa-asg-11527hsa-asg-11527_st1 2.51 2.76 2.11 2.79 2.54 0.31 0 hsa-asg-11515hsa-asg-11515_st2 9.93 10.3 10.1 10.1 10.1 0.18 100 hsa-asg-114hsa-asg-114_st2 7.40 7.70 7.42 6.99 7.38 0.30 0 hsa-asg-10065hsa-asg-10065_st1 2.31 2.45 2.11 2.50 2.34 0.18 0 hsa-miR-594hsa-miR-594_st2 11.2 10.9 11.2 11.4 11.2 0.22 100 hsa-miR246hsa-miR246_st2 4.79 4.25 5.00 3.52 4.39 0.66 75 hsa-miR181hsa-miR181_st2 3.36 3.34 4.20 4.51 3.85 0.59 25 hsa-asg-999hsa-asg-999_st2 6.33 6.30 5.73 6.57 6.23 0.36 100 hsa-asg-9951hsa-asg-9951_st2 2.69 1.74 2.11 4.18 2.68 1.07 0 hsa-asg-9885hsa-asg-9885_st1 6.93 6.75 7.18 7.23 7.02 0.22 100 hsa-asg-9856hsa-asg-9856_st2 2.08 2.08 2.65 0.58 1.85 0.89 0 hsa-asg-9795hsa-asg-9795_st2 5.93 5.99 5.88 5.67 5.87 0.14 25 hsa-asg-9714hsa-asg-9714_st1 3.25 3.13 4.29 5.33 4.00 1.03 25 hsa-asg-9687hsa-asg-9687_st1 1.22 1.35 1.53 1.18 1.32 0.16 0 hsa-asg-9627hsa-asg-9627_st1 9.91 9.74 10.0 9.66 9.84 0.18 100 hsa-asg-960hsa-asg-960_st1 9.39 9.58 8.62 9.32 9.23 0.42 100 hsa-asg-9530hsa-asg-9530_st2 12.6 12.6 12.6 12.6 12.6 0.01 100 hsa-asg-9521hsa-asg-9521_st2 2.08 2.08 2.90 2.15 2.30 0.40 0 hsa-asg-9484hsa-asg-9484_st2 10.0 10.1 10.1 10.0 10.1 0.08 100 hsa-asg-9474hsa-asg-9474_st2 4.75 5.26 4.67 4.78 4.87 0.27 100 hsa-asg-9457hsa-asg-9457_st1 12.3 12.4 12.3 12.2 12.3 0.08 100 hsa-asg-944hsa-asg-944_st2 2.85 2.89 3.43 3.04 3.05 0.26 0 hsa-asg-9386hsa-asg-9386_st2 10.7 10.8 10.5 11.0 10.8 0.21 100 hsa-asg-9297hsa-asg-9297_st2 3.57 3.19 3.31 4.12 3.55 0.42 0 hsa-asg-9262hsa-asg-9262_st1 7.91 7.56 7.43 7.89 7.70 0.24 25 hsa-asg-9261hsa-asg-9261_st1 12.5 12.0 12.6 12.3 12.3 0.27 100 hsa-asg-9238hsa-asg-9238_st1 9.80 9.27 9.34 9.16 9.39 0.28 100 hsa-asg-9185hsa-asg-9185_st2 9.19 9.37 8.94 9.42 9.23 0.22 100 hsa-asg-9164hsa-asg-9164_st1 8.46 8.11 7.51 7.76 7.96 0.41 75 hsa-asg-916hsa-asg-916_st1 9.84 10.1 9.78 10.0 9.94 0.16 100 hsa-asg-9146hsa-asg-9146_st1 12.9 12.7 12.9 12.6 12.8 0.15 100 hsa-asg-9078hsa-asg-9078_st2 11.7 11.6 11.2 11.5 11.5 0.22 0 hsa-asg-907hsa-asg-907_st1 7.32 7.85 7.24 7.56 7.49 0.27 100 hsa-asg-8957hsa-asg-8957_st2 9.50 9.63 9.42 9.37 9.48 0.11 100 hsa-asg-8953hsa-asg-8953_st1 10.6 10.3 10.6 10.3 10.4 0.15 100 hsa-asg-8941hsa-asg-8941_st2 9.53 9.42 9.22 8.85 9.26 0.30 75 hsa-asg-8932hsa-asg-8932_st1 7.39 6.96 7.25 6.29 6.97 0.49 100 hsa-asg-8928Has-asg-8928_st2 8.07 8.25 7.91 8.25 8.12 0.16 0 hsa-asg-88Has-asg-88_st1 11.8 11.9 11.7 11.7 11.8 0.12 0 hsa-asg-8736Has-asg-8736_st2 6.22 6.09 4.92 5.49 5.68 0.60 25 hsa-asg-8732Has-asg-8732_st1 8.74 8.29 8.71 8.13 8.47 0.30 0 hsa-asg-8617hsa-asg-8617_st1 10.5 9.86 10.2 9.76 10.1 0.36 100 hsa-asg-8546hsa-asg-8546_st1 8.69 8.95 8.99 7.53 8.54 0.69 100 hsa-asg-8522hsa-asg-8522_st2 7.73 7.81 7.52 7.77 7.71 0.13 100 hsa-asg-8520hsa-asg-8520_st1 10.6 10.4 10.5 10.4 10.5 0.09 100 hsa-asg-8454hsa-asg-8454_st1 4.24 3.52 4.38 3.82 3.99 0.40 50 hsa-asg-8435hsa-asg-8435_st2 8.21 8.04 7.37 7.34 7.74 0.45 75 hsa-asg-8316hsa-asg-8316_st1 10.1 9.98 10.2 10.1 10.1 0.12 100 hsa-asg-8290hsa-asg-8290_st1 9.19 9.14 9.12 9.31 9.19 0.09 100 hsa-asg-826hsa-asg-826_st1 10.2 9.98 10.1 10.0 10.1 0.10 100 hsa-asg-8197hsa-asg-8197_st2 5.31 5.11 5.21 5.38 5.25 0.12 0 hsa-asg-8146hsa-asg-8146_st2 5.99 6.26 5.72 5.15 5.78 0.47 50 hsa-asg-8137hsa-asg-8137_st2 6.13 6.03 5.57 5.15 5.72 0.45 75 hsa-asg-8099hsa-asg-8099_st2 4.71 5.14 5.01 5.83 5.17 0.48 25 hsa-asg-7999hsa-asg-7999_st1 2.85 3.43 4.55 3.44 3.57 0.71 25 hsa-asg-7946hsa-asg-7946_st2 4.05 4.02 2.11 3.34 3.38 0.91 25 hsa-asg-786hsa-asg-786_st2 7.17 6.98 6.83 7.21 7.05 0.18 100 hsa-asg-7782hsa-asg-7782_st2 10.2 9.92 10.5 10.4 10.2 0.27 100 hsa-asg-7770hsa-asg-7770_st2 7.05 6.94 6.56 7.23 6.94 0.28 100 hsa-asg-7653hsa-asg-7653_st1 9.25 9.39 9.28 9.21 9.28 0.08 100 hsa-asg-7579hsa-asg-7579_st2 8.34 8.51 8.41 8.20 8.36 0.13 100 hsa-asg-7550hsa-asg-7550_st1 8.04 7.85 8.29 8.18 8.09 0.19 0 hsa-asg-7488hsa-asg-7488_st1 5.19 3.99 4.50 4.85 4.63 0.51 0 hsa-asg-7450hsa-asg-7450_st1 10.1 10.3 10.2 10.1 10.2 0.10 100 hsa-asg-7438hsa-asg-7438_st2 2.08 2.28 2.82 2.15 2.33 0.34 0 hsa-asg-7423hsa-asg-7423_st2 2.08 2.18 1.84 2.92 2.26 0.46 0 hsa-asg-7422hsa-asg-7422_st1 3.13 2.76 2.82 2.65 2.84 0.20 0 hsa-asg-7351hsa-asg-7351_st2 1.54 2.83 1.00 0.89 1.56 0.89 0 hsa-asg-7293hsa-asg-7293_st1 10.3 9.74 10.2 9.98 10.0 0.29 100 hsa-asg-7292hsa-asg-7292_st1 9.64 9.85 10.1 9.72 9.83 0.21 100 hsa-asg-723hsa-asg-723_st2 2.31 2.28 3.31 3.04 2.73 0.52 0 hsa-asg-7213hsa-asg-7213_st1 9.26 10.4 9.66 9.32 9.66 0.53 100 hsa-asg-7203hsa-asg-7203_st1 8.26 8.28 7.84 7.50 7.97 0.37 100 hsa-asg-7199hsa-asg-7199_st1 9.91 9.57 9.68 9.35 9.63 0.23 100 hsa-asg-7166hsa-asg-7166_st2 6.21 6.06 6.42 6.67 6.34 0.26 50 hsa-asg-7091hsa-asg-7091_st1 7.41 7.80 7.51 7.90 7.65 0.23 100 hsa-asg-7063hsa-asg-7063_st1 8.81 8.16 8.44 8.22 8.41 0.29 100 hsa-asg-7027hsa-asg-7027_st2 7.80 8.00 7.51 7.78 7.77 0.20 25 hsa-asg-6898hsa-asg-6898_st2 3.13 2.45 2.90 3.04 2.88 0.30 0 hsa-asg-6788hsa-asg-6788_st1 12.0 11.4 11.7 11.5 11.7 0.26 100 hsa-asg-6773hsa-asg-6773_st1 4.18 3.83 4.29 2.92 3.80 0.62 0 hsa-asg-6765hsa-asg-6765_st1 5.78 4.92 5.65 5.76 5.53 0.41 50 hsa-asg-6532hsa-asg-6532_st1 3.13 3.02 3.05 4.18 3.34 0.56 25 hsa-asg-6503hsa-asg-6503_st1 3.47 3.83 3.73 3.60 3.66 0.16 0 hsa-asg-6500hsa-asg-6500_st1 8.14 7.90 7.83 7.48 7.83 0.27 100 hsa-asg-6488hsa-asg-6488_st1 5.93 6.39 6.49 6.72 6.38 0.33 75 hsa-asg-6449hsa-asg-6449_st1 3.91 5.06 5.23 2.65 4.21 1.19 50 hsa-asg-641hsa-asg-641_st2 1.54 2.28 2.11 2.65 2.14 0.46 0 hsa-asg-6383hsa-asg-6383_st1 8.24 8.41 8.01 8.24 8.23 0.16 0 hsa-asg-6371hsa-asg-6371_st2 10.9 10.8 10.6 10.9 10.8 0.16 100 hsa-asg-6367hsa-asg-6367_st1 12.1 11.8 11.8 11.7 11.8 0.16 100 hsa-asg-6346hsa-asg-6346_st1 9.86 9.61 9.69 9.74 9.72 0.10 100 hsa-asg-6339hsa-asg-6339_st1 9.95 10.0 9.64 9.73 9.85 0.21 100 hsa-asg-6282hsa-asg-6282_st1 8.34 8.22 8.26 8.01 8.21 0.14 100 hsa-asg-6211hsa-asg-6211_st2 9.65 9.67 9.76 9.71 9.70 0.05 100 hsa-asg-6165hsa-asg-6165_st2 4.57 4.35 3.48 4.18 4.14 0.47 25 hsa-asg-6116hsa-asg-6116_st2 2.85 3.34 5.45 4.33 3.99 1.15 50 hsa-asg-6071hsa-asg-6071_st2 6.69 6.80 6.82 6.57 6.72 0.11 100 hsa-asg-6018hsa-asg-6018_st2 10.3 10.3 10.2 9.98 10.2 0.17 100 hsa-asg-584_st1hsa-asg-584_st1 3.13 3.19 2.65 1.18 2.54 0.93 0 hsa-asg-5810hsa-asg-5810_st2 10.0 10.1 10.0 10.3 10.1 0.13 100 hsa-asg-5758hsa-asg-5758_st2 1.54 4.25 2.34 2.50 2.66 1.14 25 hsa-asg-5691hsa-asg-5691_st2 9.59 9.96 9.48 9.81 9.71 0.22 100 hsa-asg-551hsa-asg-551_st2 8.06 8.73 7.83 8.36 8.25 0.39 100 hsa-asg-5326hsa-asg-5326_st2 8.20 7.96 8.26 7.65 8.02 0.28 100 hsa-asg-529hsa-asg-529_st1 4.83 3.68 3.59 3.34 3.86 0.66 25 hsa-asg-5240hsa-asg-5240_st1 3.36 3.60 3.54 3.52 3.51 0.10 75 hsa-asg-5227hsa-asg-5227_st1 5.16 4.22 3.64 4.38 4.35 0.63 0 hsa-asg-5213hsa-asg-5213_st2 5.75 6.52 6.65 4.42 5.84 1.02 50 hsa-asg-5208hsa-asg-5208_st1 8.37 8.68 8.39 8.50 8.49 0.14 100 hsa-asg-5141hsa-asg-5141_st1 5.92 5.67 5.80 4.74 5.53 0.54 25 hsa-asg-5109hsa-asg-5109_st1 11.1 11.3 10.8 10.9 11.0 0.24 100 hsa-asg-4974hsa-asg-4974_st1 1.83 2.45 2.11 2.65 2.26 0.36 0 hsa-asg-497hsa-asg-497_st2 5.47 4.67 4.44 6.18 5.19 0.79 25 hsa-asg-4844hsa-asg-4844_st2 9.97 9.65 10.1 9.87 9.90 0.19 100 hsa-asg-4840hsa-asg-4840_st1 3.75 3.24 5.05 3.60 3.91 0.79 25 hsa-asg-4834hsa-asg-4834_st1 9.25 9.13 9.20 8.99 9.14 0.11 50 hsa-asg-4828hsa-asg-4828_st1 10.0 9.85 9.83 10.2 10.0 0.20 100 hsa-asg-4808hsa-asg-4808_st1 7.81 7.40 8.09 7.38 7.67 0.35 50 hsa-asg-471hsa-asg-471_st1 10.7 10.7 10.7 10.9 10.7 0.09 100 hsa-asg-4703hsa-asg-4703_st2 8.16 7.55 7.02 7.61 7.59 0.46 100 hsa-asg-4441hsa-asg-4441_st1 6.55 6.50 6.98 6.76 6.70 0.22 0 hsa-asg-4426hsa-asg-4426_st2 5.64 5.74 4.70 5.85 5.48 0.53 100 hsa-asg-4284hsa-asg-4284_st1 3.83 2.61 3.31 3.34 3.28 0.50 0 hsa-asg-4203hsa-asg-4203_st1 3.75 4.19 3.90 4.23 4.02 0.23 50 hsa-asg-4164hsa-asg-4164_st1 3.25 3.24 3.43 3.68 3.40 0.21 0 hsa-asg-4159hsa-asg-4159_st2 4.12 3.96 3.73 3.75 3.89 0.18 75 hsa-asg-4112hsa-asg-4112_st1 3.75 4.94 5.47 3.60 4.44 0.91 50 hsa-asg-4077hsa-asg-4077_st1 2.31 2.61 1.19 2.50 2.15 0.66 0 hsa-asg-4031hsa-asg-4031_st2 10.2 10.2 10.4 10.0 10.2 0.15 100 hsa-asg-3946hsa-asg-3946_st2 9.84 9.81 9.41 8.98 9.51 0.40 100 hsa-asg-3728hsa-asg-3728_st1 8.00 7.59 7.62 7.59 7.70 0.20 25 hsa-asg-3722hsa-asg-3722_st1 9.77 10.2 10.0 10.0 10.0 0.19 100 hsa-asg-3715hsa-asg-3715_st1 10.2 10.5 9.98 9.96 10.1 0.25 100 hsa-asg-3703hsa-asg-3703_st1 11.3 11.3 10.9 11.1 11.1 0.20 100 hsa-asg-3691hsa-asg-3691_st1 9.68 9.76 9.68 9.23 9.59 0.24 100 hsa-asg-3654hsa-asg-3654_st1 6.42 7.42 7.11 7.36 7.08 0.46 0 hsa-asg-3565hsa-asg-3565_st2 8.62 8.99 9.06 9.09 8.94 0.22 25 hsa-asg-3548hsa-asg-3548_st1 6.58 5.86 5.75 6.60 6.20 0.46 0 hsa-asg-3514hsa-asg-3514_st1 4.75 3.89 3.73 2.50 3.72 0.93 25 hsa-asg-3290hsa-asg-3290_st1 9.36 9.13 9.05 9.15 9.17 0.14 50 hsa-asg-3240hsa-asg-3240_st1 11.5 11.7 11.7 11.7 11.7 0.14 0 hsa-asg-3168hsa-asg-3168_st1 3.75 4.65 3.64 5.44 4.37 0.85 50 hsa-asg-3118hsa-asg-3118_st2 3.25 4.30 2.74 2.65 3.23 0.76 25 hsa-asg-3112hsa-asg-3112_st1 9.58 9.35 9.79 9.32 9.51 0.22 100 hsa-asg-3101hsa-asg-3101_st2 8.65 8.70 8.51 8.65 8.63 0.08 100 hsa-asg-3083hsa-asg-3083_st1 4.66 3.89 2.82 4.12 3.88 0.77 25 hsa-asg-3070hsa-asg-3070_st2 2.08 2.96 3.43 2.50 2.74 0.58 0 hsa-asg-305hsa-asg-305_st1 1.83 2.28 2.55 2.50 2.29 0.33 0 hsa-asg-3046hsa-asg-3046_st2 9.42 9.36 9.10 9.30 9.30 0.14 100 hsa-asg-304hsa-asg-304_st2 7.15 6.39 6.43 5.96 6.49 0.49 100 hsa-asg-3014hsa-asg-3014_st2 8.29 8.39 8.19 8.25 8.28 0.08 100 hsa-asg-2940hsa-asg-2940_st1 3.91 3.79 3.73 4.38 3.95 0.29 0 hsa-asg-2930hsa-asg-2930_st1 2.51 2.08 2.98 2.92 2.62 0.42 0 hsa-asg-2924hsa-asg-2924_st1 1.83 2.76 1.36 −0.02 1.48 1.16 0 hsa-asg-2906hsa-asg-2906_st2 11.4 11.3 11.3 11.42 11.4 0.06 100 hsa-asg-2886hsa-asg-2886_st2 7.92 8.45 7.92 7.86 8.04 0.28 100 hsa-asg-2855hsa-asg-2855_st2 2.51 2.61 1.84 2.33 2.32 0.34 0 hsa-asg-278hsa-asg-278_st1 9.10 8.49 8.92 8.69 8.80 0.27 25 hsa-asg-2563hsa-asg-2563_st2 8.80 8.96 8.84 8.82 8.86 0.07 100 hsa-asg-251hsa-asg-251_st1 4.12 3.96 3.98 4.28 4.09 0.15 75 hsa-asg-247hsa-asg-247_st1 8.44 8.47 8.41 8.14 8.37 0.15 100 hsa-asg-2459hsa-asg-2459_st1 2.08 2.89 2.11 2.33 2.35 0.38 0 hsa-asg-2449hsa-asg-2449_st1 7.65 4.55 6.35 4.42 5.74 1.55 50 hsa-asg-2437hsa-asg-2437_st1 8.20 8.23 7.86 8.13 8.10 0.17 100 hsa-asg-2433hsa-asg-2433_st2 4.12 4.49 3.86 4.01 4.12 0.27 25 hsa-asg-2401hsa-asg-2401_st2 9.50 9.28 9.27 9.34 9.35 0.11 100 hsa-asg-2371hsa-asg-2371_st1 2.31 1.35 1.84 2.15 1.91 0.42 0 hsa-asg-2232hsa-asg-2232_st1 1.83 1.86 2.34 1.94 1.99 0.24 0 hsa-asg-2076hsa-asg-2076_st1 2.08 3.13 2.74 3.25 2.80 0.53 25 hsa-asg-1978hsa-asg-1978_st2 3.91 3.02 3.82 3.34 3.52 0.42 0 hsa-asg-1974hsa-asg-1974_st1 2.69 2.89 2.74 2.79 2.78 0.09 0 hsa-asg-1937hsa-asg-1937_st1 1.83 1.05 1.19 2.92 1.75 0.85 0 hsa-asg-1919hsa-asg-1919_st1 9.27 9.16 9.62 9.03 9.27 0.25 100 hsa-asg-1886hsa-asg-1886_st2 11.3 10.9 11.0 10.81 11.0 0.22 100 hsa-asg-1832hsa-asg-1832_st2 8.01 8.54 7.95 8.21 8.18 0.27 0 hsa-asg-1807hsa-asg-1807_st2 0.86 1.05 1.00 2.33 1.31 0.68 0 hsa-asg-1797hsa-asg-1797_st2 9.92 9.76 9.76 9.74 9.80 0.08 100 hsa-asg-1726hsa-asg-1726_st1 11.0 10.9 11.0 11.16 11.0 0.10 100 hsa-asg-1658hsa-asg-1658_st1 5.09 5.77 5.62 5.57 5.51 0.29 50 hsa-asg-1557hsa-asg-1557_st1 3.91 3.52 4.32 3.15 3.72 0.51 25 hsa-asg-1532hsa-asg-1532_st1 8.62 7.73 7.82 8.25 8.10 0.41 75 hsa-asg-145hsa-asg-145_st2 10.1 10.0 9.75 9.97 9.99 0.19 100 hsa-asg-1448hsa-asg-1448_st1 8.17 7.58 7.79 7.17 7.68 0.42 0 hsa-asg-14237hsa-asg-14237_st2 9.20 8.77 8.50 8.98 8.86 0.30 100 hsa-asg-142hsa-asg-142_st1 9.64 9.46 9.08 9.20 9.35 0.25 50 hsa-asg-14197hsa-asg-14197_st1 13.4 13.2 13.5 13.49 13.4 0.14 100 hsa-asg-14189hsa-asg-14189_st2 14.6 14.4 14.6 14.49 14.5 0.11 100 hsa-asg-14135hsa-asg-14135_st1 3.36 3.83 3.19 3.68 3.51 0.29 0 hsa-asg-14053hsa-asg-14053_st2 6.12 6.06 6.23 5.96 6.09 0.11 0 hsa-asg-14029hsa-asg-14029_st2 7.01 6.21 6.52 6.11 6.46 0.41 100 hsa-asg-14026hsa-asg-14026_st1 2.99 3.13 2.55 3.75 3.11 0.50 0 hsa-asg-1398hsa-asg-1398_st2 4.95 4.69 4.02 4.67 4.58 0.40 50 hsa-asg-13973hsa-asg-13973_st1 7.98 7.47 8.52 8.25 8.06 0.45 50 hsa-asg-13963hsa-asg-13963_st2 8.75 8.70 8.45 8.04 8.48 0.32 0 hsa-asg-13895hsa-asg-13895_st1 4.30 4.19 4.13 4.07 4.17 0.10 100 hsa-asg-13808hsa-asg-13808_st1 3.83 4.73 3.98 5.30 4.46 0.69 50 hsa-asg-13736hsa-asg-13736_st2 8.87 8.80 8.62 8.60 8.72 0.13 25 hsa-asg-13705hsa-asg-13705_st1 3.25 3.13 2.34 2.65 2.84 0.42 0 hsa-asg-1369hsa-asg-1369_st1 1.83 1.86 2.74 1.71 2.04 0.47 0 hsa-asg-13606hsa-asg-13606_st2 12.1 11.7 11.8 11.80 11.9 0.19 100 hsa-asg-13604hsa-asg-13604_st1 11.0 11.2 11.1 11.04 11.1 0.11 100 hsa-asg-1360hsa-asg-1360_st1 9.01 8.96 8.94 8.97 8.97 0.03 25 hsa-asg-13501hsa-asg-13501_st1 6.56 7.42 6.40 6.08 6.62 0.57 100 hsa-asg-13334hsa-asg-13334_st2 3.75 4.17 3.31 5.33 4.14 0.87 0 hsa-asg-13306hsa-asg-13306_st2 12.5 12.3 12.1 12.49 12.3 0.20 100 hsa-asg-13294hsa-asg-13294_st2 4.52 4.51 4.20 4.47 4.42 0.15 0 hsa-asg-13197hsa-asg-13197_st1 2.08 2.28 2.34 4.01 2.68 0.89 0 hsa-asg-13083hsa-asg-13083_st1 9.50 9.54 8.85 9.40 9.32 0.32 50 hsa-asg-12922hsa-asg-12922_st1 6.94 7.41 6.34 7.19 6.97 0.47 100 hsa-asg-1270hsa-asg-1270_st2 3.75 5.31 3.73 3.68 4.12 0.80 25 hsa-asg-12687hsa-asg-12687_st1 9.08 8.92 9.07 9.37 9.11 0.19 50 hsa-asg-12602hsa-asg-12602_st1 1.54 1.62 1.53 1.94 1.66 0.19 0 hsa-asg-12556hsa-asg-12556_st1 10.9 10.7 10.4 10.33 10.6 0.26 100 hsa-asg-12549hsa-asg-12549_st1 3.66 4.94 2.34 3.34 3.57 1.07 25 hsa-asg-12531hsa-asg-12531_st1 6.76 4.32 4.85 5.04 5.24 1.05 25 hsa-asg-12463hsa-asg-12463_st2 1.83 2.08 1.84 1.94 1.92 0.12 0 hsa-asg-12461hsa-asg-12461_st1 0.86 1.86 1.84 2.33 1.72 0.62 0 hsa-asg-12460hsa-asg-12460_st2 7.92 8.60 8.95 8.51 8.49 0.42 100 hsa-asg-12441hsa-asg-12441_st1 11.0 10.8 10.8 10.80 10.8 0.13 100 hsa-asg-12421hsa-asg-12421_st1 9.94 10.1 9.58 9.73 9.86 0.26 100 hsa-asg-12356hsa-asg-12356_st1 9.24 8.90 9.06 9.24 9.11 0.17 100 hsa-asg-12344hsa-asg-12344_st1 10.1 9.65 9.57 9.56 9.75 0.30 50 hsa-asg-12343hsa-asg-12343_st1 7.32 6.59 6.82 6.26 6.75 0.44 100 hsa-asg-12320hsa-asg-12320_st2 2.51 2.54 1.53 3.34 2.48 0.74 0 hsa-asg-12286hsa-asg-12286_st2 9.32 9.24 8.82 8.67 9.01 0.32 50 hsa-asg-12150hsa-asg-12150_st1 10.9 10.9 11.0 10.56 10.8 0.19 100 hsa-asg-12045hsa-asg-12045_st2 3.25 2.83 3.98 3.89 3.49 0.55 0 hsa-asg-12005hsa-asg-12005_st1 9.84 9.22 9.59 9.42 9.52 0.26 100 hsa-asg-11973hsa-asg-11973_st2 9.02 8.75 9.27 8.65 8.92 0.28 25 hsa-asg-11969hsa-asg-11969_st1 8.89 8.78 8.42 8.82 8.73 0.21 25 hsa-asg-11956hsa-asg-11956_st1 8.71 8.42 8.38 8.20 8.43 0.21 0 hsa-asg-11945hsa-asg-11945_st1 10.0 9.87 9.64 9.70 9.81 0.18 100 hsa-asg-11931hsa-asg-11931_st1 3.47 4.67 3.86 2.92 3.73 0.74 0 hsa-asg-11930hsa-asg-11930_st1 4.83 5.36 5.09 5.88 5.29 0.45 100 hsa-asg-11898hsa-asg-11898_st2 7.87 8.08 7.50 7.76 7.80 0.24 100 hsa-asg-1189hsa-asg-1189_st1 7.61 9.05 8.28 8.12 8.26 0.60 75 hsa-asg-11877hsa-asg-11877_st1 7.07 6.75 6.47 6.82 6.78 0.24 0 hsa-asg-11797hsa-asg-11797_st1 8.12 8.21 8.34 7.84 8.13 0.21 0 hsa-asg-1174hsa-asg-1174_st2 9.88 9.61 9.72 9.65 9.72 0.12 100 hsa-asg-11643hsa-asg-11643_st1 2.69 2.89 3.19 3.15 2.98 0.23 0 hsa-asg-11623hsa-asg-11623_st1 11.6 11.2 11.5 11.33 11.4 0.16 0 hsa-asg-11617hsa-asg-11617_st2 10.0 10.5 10.3 10.49 10.3 0.24 100 hsa-asg-11612hsa-asg-11612_st1 7.82 7.99 7.93 7.89 7.91 0.07 0 hsa-asg-11606hsa-asg-11606_st1 11.3 11.1 11.5 11.13 11.3 0.18 100 hsa-asg-11580hsa-asg-11580_st2 1.54 1.62 2.55 2.50 2.05 0.55 0 hsa-asg-11555hsa-asg-11555_st2 4.75 4.84 3.90 4.59 4.52 0.42 75 hsa-asg-11521hsa-asg-11521_st1 9.76 9.35 8.96 8.91 9.25 0.39 75 hsa-asg-11513hsa-asg-11513_st1 9.28 9.57 9.48 9.26 9.40 0.15 75 hsa-asg-11193hsa-asg-11193_st1 12.2 12.2 11.9 11.98 12.1 0.17 75 hsa-asg-11128hsa-asg-11128_st2 4.05 3.89 3.73 4.33 4.00 0.25 50 hsa-asg-11112hsa-asg-11112_st2 11.5 11.2 11.4 11.39 11.4 0.13 100 hsa-asg-11002hsa-asg-11002_st2 3.47 2.37 3.37 3.68 3.22 0.58 0 hsa-asg-10958hsa-asg-10958_st2 1.54 2.37 2.34 2.50 2.19 0.44 0 hsa-asg-10901hsa-asg-10901_st1 7.24 6.90 7.15 6.89 7.04 0.18 100 hsa-asg-10810hsa-asg-10810_st1 3.36 3.43 3.82 3.34 3.49 0.22 0 hsa-asg-1072hsa-asg-1072_st1 2.08 3.43 2.55 3.89 2.99 0.82 50 hsa-asg-10639hsa-asg-10639_st1 11.7 11.9 11.7 11.59 11.7 0.12 100 hsa-asg-10517hsa-asg-10517_st2 1.83 2.18 1.36 2.65 2.01 0.54 0 hsa-asg-105hsa-asg-105_st1 3.36 4.17 3.25 2.33 3.28 0.75 0 hsa-asg-10487hsa-asg-10487_st1 3.25 4.22 6.37 4.47 4.58 1.30 25 hsa-asg-10486hsa-asg-10486_st2 2.69 3.13 3.31 2.50 2.91 0.38 50 hsa-asg-10464hsa-asg-10464_st2 2.69 3.43 5.40 4.91 4.11 1.26 50 hsa-asg-10392hsa-asg-10392_st1 10.5 9.84 10.0 10.02 10.1 0.30 100 hsa-asg-10280hsa-asg-10280_st1 2.31 3.60 2.74 3.82 3.12 0.71 0 hsa-asg-10278hsa-asg-10278_st2 2.51 2.08 2.55 2.33 2.37 0.21 0 hsa-asg-1026hsa-asg-1026_st2 5.47 5.58 5.74 5.85 5.66 0.17 100 hsa-asg-10247hsa-asg-10247_st1 2.08 2.45 2.11 1.94 2.15 0.22 0 hsa-asg-1020hsa-asg-1020_st2 8.76 8.76 8.67 8.46 8.66 0.14 0 hsa-asg-1018hsa-asg-1018_st2 6.18 6.90 6.65 6.67 6.60 0.30 100 hsa-asg-10131hsa-asg-10131_st2 3.57 3.13 4.32 3.34 3.59 0.52 25 hsa-miR-224hsa-asg-14247_st1 5.02 4.65 5.53 5.98 5.30 0.58 100 hsa-miR-99bhsa-asg-14196_st2 8.48 8.15 8.73 8.43 8.45 0.24 0 LNpos vs LNneg LNposFold MicroRNA 2 3 4 Mean SD % ttest Log2Diff Change hsa-let-7e 10.6 11.211.0 11.0 0.28 100 1.36E−02 0.90 2 hsa-miR-103 11.3 12.7 12.0 12.0 0.66100 3.39E−02 1.03 2 hsa-miR-106b 9.40 9.81 9.37 9.53 0.25 100 2.68E−032.09 4 hsa-miR-107 11.1 12.5 11.9 11.8 0.68 100 3.05E−02 1.15 2hsa-miR-128a 7.58 8.49 7.24 7.77 0.65 100 3.17E−02 1.58 3 hsa-miR-130b6.61 7.49 7.70 7.27 0.58 100 2.09E−02 1.11 2 hsa-miR-141 7.90 10.2 8.688.93 1.18 100 1.58E−04 7.05 132 hsa-miR-145 11.9 11.1 12.0 11.7 0.49 1004.96E−02 −0.70 2 hsa-miR-148a 11.2 10.0 10.4 10.5 0.63 100 5.54E−03 2.124 hsa-miR-148b 6.16 8.19 7.87 7.41 1.09 100 3.58E−02 2.10 4 hsa-miR-1517.14 8.42 7.83 7.80 0.64 100 2.88E−02 1.28 2 hsa-miR-181a 10.0 11.2 10.410.5 0.62 100 3.76E−02 1.10 2 hsa-miR-181a* 6.37 7.12 6.81 6.77 0.38 1003.17E−02 3.22 9 hsa-miR-181d 6.77 8.72 6.91 7.47 1.09 100 2.47E−03 3.249 hsa-miR-182 8.25 11.0 11.0 10.0 1.60 100 6.82E−04 6.39 84 hsa-miR-1833.60 8.39 7.97 6.65 2.65 67 2.56E−02 4.36 21 hsa-miR-189 2.26 4.83 2.853.32 1.35 0 3.76E−02 2.15 4 hsa-miR-191 12.2 12.3 13.0 12.5 0.45 1008.79E−03 1.20 2 hsa-miR-193b 9.82 9.91 10.8 10.1 0.55 100 1.07E−02 2.104 hsa-miR-196a 8.79 10.4 8.12 9.12 1.19 100 3.55E−03 6.25 76hsa-miR-199a 9.75 10.7 10.9 10.4 0.62 100 2.47E−02 1.04 2 hsa-miR-200a9.05 8.38 10.2 9.22 0.93 100 3.29E−05 6.75 107 hsa-miR-200a* 6.26 5.135.18 5.52 0.64 33 1.64E−02 2.08 4 hsa-miR-200b 11.2 9.92 11.7 10.9 0.91100 3.45E−05 8.33 322 hsa-miR-200c 11.4 12.6 11.2 11.7 0.77 100 3.18E−045.79 56 hsa-miR-203 7.23 7.97 8.59 7.93 0.68 100 8.39E−05 4.98 32hsa-miR-21 11.9 14.0 13.8 13.2 1.18 100 7.59E−03 2.81 7 hsa-miR-218 5.603.48 6.34 5.14 1.48 67 4.95E−02 2.58 6 hsa-miR-22 9.74 10.8 10.8 10.40.63 100 7.20E−03 1.40 3 hsa-miR-224 6.32 8.37 10.3 8.34 2.01 1003.74E−02 3.03 8 hsa-miR-24 10.9 12.2 11.6 11.6 0.62 100 4.48E−02 1.05 2hsa-miR-25 10.7 10.7 10.9 10.8 0.10 100 1.62E−04 1.01 2 hsa-miR-27a 9.5310.7 10.4 10.2 0.65 100 6.67E−03 1.61 3 hsa-miR-30a-3p 7.36 8.29 8.448.03 0.59 100 8.88E−03 2.26 5 hsa-miR-30a-5p 10.3 11.4 11.1 10.9 0.60100 1.17E−02 1.23 2 hsa-miR-30e-5p 8.48 9.66 10.1 9.43 0.85 100 4.47E−021.26 2 hsa-miR-34a 7.55 8.07 8.89 8.17 0.68 100 3.24E−02 1.61 3hsa-miR-34b 3.95 6.05 6.12 5.37 1.23 67 5.12E−03 2.93 8 hsa-miR-363 7.247.92 7.75 7.64 0.35 100 2.29E−02 0.95 2 hsa-miR-373* 6.95 7.23 7.39 7.190.23 100 2.26E−02 −0.54 1 hsa-miR-375 10.7 10.4 10.2 10.4 0.23 1001.60E−03 8.35 325 hsa-miR-376a 4.53 2.71 3.63 3.62 0.91 33 1.05E−02 1.874 hsa-miR-421 5.06 6.36 6.43 5.95 0.78 100 1.38E−03 3.57 12hsa-miR-425-5p 9.73 10.8 11.2 10.6 0.78 100 2.38E−03 2.24 5 hsa-miR-4297.13 5.83 7.50 6.82 0.88 100 3.32E−03 4.05 17 hsa-miR-452 6.66 6.23 7.386.76 0.58 100 1.30E−02 1.80 3 hsa-miR-489 7.99 4.71 3.83 5.51 2.20 334.02E−02 3.13 9 hsa-miR-508 4.90 4.37 5.33 4.87 0.48 67 1.63E−02 1.29 2hsa-miR-539 1.41 2.02 1.36 1.60 0.37 0 4.23E−02 −0.56 1 hsa-miR-542-5p5.26 5.21 5.09 5.19 0.09 0 9.84E−04 3.64 12 hsa-miR-654 6.26 6.84 6.536.54 0.29 0 4.55E−02 −0.98 2 hsa-miR-93 9.41 8.57 9.64 9.21 0.56 1004.95E−03 1.41 3 hsa-miR-98 7.23 8.80 7.48 7.83 0.84 100 1.99E−02 1.63 3hsa-miR-99b 9.34 9.87 9.37 9.53 0.29 0 1.53E−03 1.21 2 hsa-asg-9769 4.174.46 5.82 4.82 0.88 33 9.26E−03 2.08 4 hsa-asg-8901 12.3 12.4 12.6 12.40.15 100 2.52E−02 0.26 1 hsa-asg-860 3.00 3.79 3.23 3.34 0.41 0 4.07E−020.69 2 hsa-asg-6457 3.00 2.40 1.79 2.40 0.61 0 4.07E−02 −0.93 2hsa-asg-5021 2.48 4.55 4.79 3.94 1.27 0 3.68E−02 1.91 4 hsa-asg-141753.00 3.72 4.46 3.73 0.73 33 1.66E−02 1.35 3 hsa-asg-13258 6.93 6.94 6.996.95 0.04 100 3.47E−02 1.40 3 hsa-asg-13254 4.98 4.46 6.43 5.29 1.02 333.56E−02 2.72 7 hsa-asg-13205 5.09 6.99 6.49 6.19 0.99 67 6.74E−03 2.877 hsa-asg-12885 3.95 3.79 3.94 3.90 0.09 100 2.35E−04 1.00 2hsa-asg-12471 2.26 2.71 2.99 2.66 0.37 0 3.94E−02 0.62 2 hsa-asg-1237210.7 10.6 10.6 10.6 0.05 100 4.22E−03 0.32 1 hsa-asg-11896 12.3 12.112.0 12.1 0.16 100 3.91E−02 0.33 1 hsa-asg-11527 3.39 4.37 4.36 4.040.56 67 6.17E−03 1.50 3 hsa-asg-11515 9.39 9.64 9.77 9.60 0.20 1001.29E−02 −0.54 1 hsa-asg-114 6.40 6.93 6.82 6.72 0.28 0 3.06E−02 −0.66 2hsa-asg-10065 3.39 2.98 3.12 3.16 0.21 0 2.43E−03 0.82 2 hsa-miR-59411.6 11.8 11.6 11.6 0.10 100 2.35E−02 0.46 1 hsa-miR246 5.23 5.62 5.905.58 0.34 100 3.67E−02 1.20 2 hsa-miR181 2.67 2.85 2.70 2.74 0.10 02.58E−02 −1.11 2 hsa-asg-999 6.77 6.99 7.17 6.98 0.20 100 2.40E−02 0.742 hsa-asg-9951 4.30 5.72 4.14 4.72 0.87 33 4.39E−02 2.04 4 hsa-asg-98855.96 5.96 5.89 5.94 0.04 100 4.67E−04 −1.08 2 hsa-asg-9856 3.95 3.095.04 4.03 0.98 33 2.72E−02 2.18 5 hsa-asg-9795 4.10 4.95 3.63 4.23 0.670 4.36E−03 −1.64 3 hsa-asg-9714 2.26 2.22 2.62 2.37 0.22 0 4.58E−02−1.63 3 hsa-asg-9687 2.02 2.22 1.91 2.05 0.16 0 1.75E−03 0.73 2hsa-asg-9627 9.40 9.02 9.55 9.32 0.28 100 2.79E−02 −0.52 1 hsa-asg-9607.51 8.79 8.27 8.19 0.65 67 4.78E−02 −1.04 2 hsa-asg-9530 12.7 12.7 12.712.7 0.02 100 2.47E−03 0.07 1 hsa-asg-9521 2.84 3.72 3.63 3.40 0.48 02.16E−02 1.10 2 hsa-asg-9484 9.30 9.86 9.41 9.52 0.29 100 1.14E−02 −0.581 hsa-asg-9474 4.30 4.50 4.49 4.43 0.11 0 4.76E−02 −0.43 1 hsa-asg-945711.8 11.8 11.7 11.8 0.04 100 1.37E−04 −0.52 1 hsa-asg-944 4.48 3.93 3.543.98 0.47 33 2.00E−02 0.93 2 hsa-asg-9386 10.5 10.4 10.5 10.5 0.05 1004.74E−02 −0.32 1 hsa-asg-9297 6.44 4.78 5.66 5.63 0.83 67 6.90E−03 2.084 hsa-asg-9262 8.60 8.57 8.65 8.61 0.04 100 1.49E−03 0.91 2 hsa-asg-926112.8 12.7 12.8 12.8 0.04 100 4.62E−02 0.42 1 hsa-asg-9238 8.94 8.98 8.758.89 0.12 100 3.74E−02 −0.50 1 hsa-asg-9185 8.61 8.81 8.92 8.78 0.16 1003.06E−02 −0.45 1 hsa-asg-9164 7.12 7.21 7.23 7.19 0.06 33 2.57E−02 −0.772 hsa-asg-916 9.68 9.56 9.34 9.52 0.17 100 2.26E−02 −0.42 1 hsa-asg-914613.1 13.0 13.2 13.1 0.12 100 2.90E−02 0.33 1 hsa-asg-9078 10.9 11.2 11.011.0 0.15 0 2.13E−02 −0.49 1 hsa-asg-907 8.59 8.05 8.12 8.25 0.29 1001.70E−02 0.76 2 hsa-asg-8957 8.70 8.53 8.93 8.72 0.20 100 1.28E−03 −0.762 hsa-asg-8953 11.2 10.8 10.7 10.9 0.27 100 2.59E−02 0.49 1 hsa-asg-89418.58 8.61 8.95 8.72 0.21 67 4.49E−02 −0.54 1 hsa-asg-8932 5.92 5.79 6.185.96 0.20 100 2.08E−02 −1.01 2 hsa-asg-8928 7.68 7.75 7.41 7.61 0.18 01.10E−02 −0.50 1 hsa-asg-88 11.1 11.2 11.2 11.2 0.05 0 5.80E−04 −0.58 1hsa-asg-8736 4.17 2.57 3.94 3.56 0.87 0 1.19E−02 −2.12 4 hsa-asg-87326.73 7.70 7.48 7.30 0.51 0 1.22E−02 −1.16 2 hsa-asg-8617 10.5 11.1 10.610.7 0.28 100 4.45E−02 0.67 2 hsa-asg-8546 7.09 6.53 7.70 7.11 0.59 333.43E−02 −1.43 3 hsa-asg-8522 7.45 6.86 6.92 7.08 0.33 100 1.53E−02−0.63 2 hsa-asg-8520 10.1 9.91 10.2 10.1 0.19 100 1.08E−02 −0.41 1hsa-asg-8454 5.38 4.63 4.65 4.89 0.43 100 3.55E−02 0.90 2 hsa-asg-84357.19 6.21 6.96 6.78 0.51 0 4.67E−02 −0.95 2 hsa-asg-8316 9.53 9.67 9.929.71 0.20 100 1.47E−02 −0.43 1 hsa-asg-8290 8.56 8.71 8.34 8.54 0.18 1001.40E−03 −0.65 2 hsa-asg-826 9.67 9.35 9.81 9.61 0.24 100 1.18E−02 −0.501 hsa-asg-8197 5.88 5.72 5.38 5.66 0.25 67 3.40E−02 0.41 1 hsa-asg-81466.95 6.42 7.72 7.03 0.65 100 3.14E−02 1.25 2 hsa-asg-8137 6.72 6.70 7.226.88 0.29 100 1.21E−02 1.16 2 hsa-asg-8099 2.84 3.76 4.01 3.54 0.61 01.03E−02 −1.63 3 hsa-asg-7999 5.47 5.01 4.89 5.12 0.31 100 1.75E−02 1.563 hsa-asg-7946 2.02 1.80 1.91 1.91 0.11 0 4.15E−02 −1.47 3 hsa-asg-7865.67 6.49 6.48 6.21 0.47 67 2.01E−02 −0.84 2 hsa-asg-7782 10.9 11.5 11.011.1 0.35 100 1.14E−02 0.91 2 hsa-asg-7770 7.57 7.77 7.35 7.56 0.21 1002.51E−02 0.62 2 hsa-asg-7653 9.00 9.21 9.01 9.07 0.12 100 3.92E−02 −0.211 hsa-asg-7579 7.48 7.63 7.83 7.65 0.17 100 1.48E−03 −0.72 2hsa-asg-7550 9.06 8.82 8.70 8.86 0.19 100 3.04E−03 0.77 2 hsa-asg-74886.19 5.36 6.58 6.05 0.62 67 2.12E−02 1.42 3 hsa-asg-7450 9.22 9.81 9.539.52 0.30 100 6.31E−03 −0.70 2 hsa-asg-7438 6.34 4.81 5.54 5.56 0.76 675.78E−04 3.23 9 hsa-asg-7423 3.79 3.09 3.44 3.44 0.35 0 1.43E−02 1.19 2hsa-asg-7422 3.50 3.57 3.18 3.41 0.21 0 1.46E−02 0.58 1 hsa-asg-73514.03 5.82 3.12 4.32 1.37 33 2.26E−02 2.76 7 hsa-asg-7293 9.67 9.54 9.629.61 0.07 100 4.01E−02 −0.48 1 hsa-asg-7292 9.52 9.14 9.18 9.28 0.21 1001.74E−02 −0.55 1 hsa-asg-723 3.60 4.27 4.20 4.02 0.37 33 1.52E−02 1.29 2hsa-asg-7213 9.25 8.05 8.26 8.52 0.64 100 4.83E−02 −1.14 2 hsa-asg-72036.81 7.00 7.42 7.08 0.31 33 2.01E−02 −0.89 2 hsa-asg-7199 9.05 9.37 8.969.13 0.21 100 3.33E−02 −0.50 1 hsa-asg-7166 3.27 5.10 5.03 4.47 1.04 01.59E−02 −1.87 4 hsa-asg-7091 6.09 6.88 6.54 6.50 0.40 67 4.64E−03 −1.152 hsa-asg-7063 7.62 7.97 7.35 7.65 0.31 100 2.16E−02 −0.76 2hsa-asg-7027 7.23 7.01 7.61 7.28 0.30 33 4.79E−02 −0.49 1 hsa-asg-68984.10 4.88 5.46 4.81 0.68 67 3.49E−03 1.93 4 hsa-asg-6788 12.3 11.9 12.212.1 0.17 100 4.61E−02 0.46 1 hsa-asg-6773 5.26 4.92 4.63 4.93 0.32 333.69E−02 1.13 2 hsa-asg-6765 4.77 4.65 4.60 4.67 0.09 0 1.76E−02 −0.85 2hsa-asg-6532 4.58 4.37 4.14 4.36 0.22 67 3.27E−02 1.02 2 hsa-asg-65034.17 4.11 4.46 4.25 0.19 0 6.10E−03 0.59 2 hsa-asg-6500 8.29 8.34 8.368.33 0.04 100 2.72E−02 0.50 1 hsa-asg-6488 6.90 7.17 7.60 7.22 0.36 1002.35E−02 0.84 2 hsa-asg-6449 1.73 2.02 1.51 1.76 0.26 0 1.88E−02 −2.46 5hsa-asg-641 2.67 4.59 3.79 3.68 0.96 33 3.56E−02 1.54 3 hsa-asg-63838.64 8.55 8.40 8.53 0.12 67 4.27E−02 0.31 1 hsa-asg-6371 10.4 10.3 10.510.4 0.09 100 1.19E−02 −0.40 1 hsa-asg-6367 11.3 11.3 11.7 11.4 0.25 1004.63E−02 −0.40 1 hsa-asg-6346 9.47 9.39 9.40 9.42 0.05 100 5.90E−03−0.30 1 hsa-asg-6339 9.47 9.33 9.50 9.43 0.09 100 2.31E−02 −0.42 1hsa-asg-6282 7.59 7.45 7.97 7.67 0.27 33 1.87E−02 −0.54 1 hsa-asg-621110.1 9.86 10.0 10.0 0.13 100 6.60E−03 0.32 1 hsa-asg-6165 2.67 2.40 2.452.51 0.15 0 2.25E−03 −1.64 3 hsa-asg-6116 2.02 2.40 1.04 1.82 0.70 03.58E−02 −2.17 5 hsa-asg-6071 5.44 5.49 6.10 5.68 0.37 33 2.78E−03 −1.042 hsa-asg-6018 8.97 9.74 9.81 9.51 0.46 100 3.30E−02 −0.71 2hsa-asg-584_st1 4.77 5.67 4.31 4.92 0.69 33 1.41E−02 2.38 5 hsa-asg-58109.90 9.62 9.97 9.83 0.18 100 3.93E−02 −0.32 1 hsa-asg-5758 0.67 0.981.04 0.89 0.20 0 4.88E−02 −1.76 3 hsa-asg-5691 9.21 8.98 9.34 9.18 0.18100 1.89E−02 −0.53 1 hsa-asg-551 7.17 7.46 7.66 7.43 0.25 100 2.59E−02−0.82 2 hsa-asg-5326 6.24 5.98 6.79 6.34 0.41 67 1.31E−03 −1.68 3hsa-asg-529 2.48 2.40 2.62 2.50 0.11 0 1.85E−02 −1.36 3 hsa-asg-52404.63 4.67 4.25 4.52 0.23 100 4.83E−04 1.01 2 hsa-asg-5227 6.05 5.64 5.665.79 0.23 100 1.37E−02 1.44 3 hsa-asg-5213 4.48 4.14 4.07 4.23 0.22 04.71E−02 −1.61 3 hsa-asg-5208 8.11 7.51 7.73 7.78 0.30 100 8.55E−03−0.71 2 hsa-asg-5141 4.73 4.59 4.51 4.61 0.11 0 3.47E−02 −0.92 2hsa-asg-5109 10.8 10.3 10.5 10.5 0.24 100 4.53E−02 −0.49 1 hsa-asg-49743.14 5.58 3.71 4.14 1.28 33 3.45E−02 1.88 4 hsa-asg-497 3.87 3.79 3.233.63 0.35 0 2.61E−02 −1.56 3 hsa-asg-4844 10.4 10.2 10.2 10.3 0.09 1001.84E−02 0.41 1 hsa-asg-4840 6.09 4.71 6.68 5.82 1.01 67 3.67E−02 1.91 4hsa-asg-4834 8.86 9.00 8.73 8.86 0.14 100 3.05E−02 −0.28 1 hsa-asg-48289.58 9.29 9.00 9.29 0.29 100 1.20E−02 −0.71 2 hsa-asg-4808 7.08 7.077.13 7.09 0.03 33 3.70E−02 −0.58 1 hsa-asg-471 10.7 10.4 10.4 10.5 0.17100 3.58E−02 −0.27 1 hsa-asg-4703 7.11 6.21 6.54 6.62 0.45 100 4.00E−02−0.97 2 hsa-asg-4441 6.98 8.16 8.04 7.72 0.65 67 2.93E−02 1.03 2hsa-asg-4426 4.94 4.50 4.01 4.48 0.47 67 4.91E−02 −1.00 2 hsa-asg-42844.30 5.24 4.07 4.54 0.62 100 2.99E−02 1.26 2 hsa-asg-4203 4.94 4.67 5.244.95 0.29 100 5.05E−03 0.93 2 hsa-asg-4164 3.87 4.50 4.01 4.13 0.33 331.52E−02 0.73 2 hsa-asg-4159 4.53 4.05 4.51 4.36 0.27 67 3.84E−02 0.47 1hsa-asg-4112 3.27 1.55 2.45 2.42 0.86 0 3.14E−02 −2.02 4 hsa-asg-40774.58 4.42 3.12 4.04 0.80 67 1.84E−02 1.89 4 hsa-asg-4031 9.93 9.53 9.719.72 0.20 100 1.01E−02 −0.53 1 hsa-asg-3946 10.2 9.99 10.2 10.1 0.15 1004.93E−02 0.64 2 hsa-asg-3728 7.23 7.28 7.00 7.17 0.15 0 1.20E−02 −0.53 1hsa-asg-3722 9.82 9.19 9.19 9.40 0.37 100 3.16E−02 −0.62 2 hsa-asg-37159.83 9.56 9.38 9.59 0.23 100 2.64E−02 −0.58 1 hsa-asg-3703 10.9 10.610.8 10.8 0.13 100 4.76E−02 −0.36 1 hsa-asg-3691 9.16 9.18 9.12 9.150.03 100 2.90E−02 −0.44 1 hsa-asg-3654 7.75 8.22 7.94 7.97 0.24 332.93E−02 0.89 2 hsa-asg-3565 8.58 8.52 8.01 8.37 0.31 67 3.65E−02 −0.561 hsa-asg-3548 7.26 6.96 7.18 7.13 0.16 33 2.08E−02 0.93 2 hsa-asg-35142.26 1.80 1.36 1.81 0.45 0 2.30E−02 −1.91 4 hsa-asg-3290 9.34 9.54 9.379.42 0.11 100 4.92E−02 0.25 1 hsa-asg-3240 11.4 11.1 11.2 11.3 0.12 01.08E−02 −0.40 1 hsa-asg-3168 3.00 2.98 2.99 2.99 0.01 0 4.02E−02 −1.383 hsa-asg-3118 2.26 1.80 1.91 1.99 0.24 0 4.36E−02 −1.24 2 hsa-asg-31128.60 9.15 9.01 8.92 0.28 100 2.64E−02 −0.59 2 hsa-asg-3101 9.16 8.789.40 9.11 0.31 100 2.86E−02 0.48 1 hsa-asg-3083 1.73 2.40 3.06 2.40 0.660 4.53E−02 −1.48 3 hsa-asg-3070 6.19 3.65 4.56 4.80 1.29 33 3.41E−022.06 4 hsa-asg-305 1.41 1.55 1.91 1.63 0.26 0 3.49E−02 −0.66 2hsa-asg-3046 10.0 9.83 9.73 9.88 0.17 100 4.27E−03 0.58 1 hsa-asg-3045.26 5.81 5.77 5.61 0.31 67 4.46E−02 −0.87 2 hsa-asg-3014 7.69 7.78 7.377.61 0.21 67 2.10E−03 −0.67 2 hsa-asg-2940 5.16 4.85 7.21 5.74 1.28 333.88E−02 1.79 3 hsa-asg-2930 3.39 4.46 3.97 3.94 0.54 0 1.42E−02 1.32 2hsa-asg-2924 6.11 5.50 3.49 5.03 1.37 67 1.36E−02 3.55 12 hsa-asg-290611.0 11.1 11.2 11.1 0.12 100 1.34E−02 −0.26 1 hsa-asg-2886 7.04 7.096.92 7.02 0.08 100 1.76E−03 −1.02 2 hsa-asg-2855 3.27 4.22 3.23 3.570.56 33 1.39E−02 1.25 2 hsa-asg-278 9.39 9.53 9.65 9.52 0.13 1007.82E−03 0.72 2 hsa-asg-2563 7.48 8.04 8.08 7.86 0.33 67 1.95E−03 −0.992 hsa-asg-251 4.58 4.46 5.03 4.69 0.30 100 1.59E−02 0.60 2 hsa-asg-2477.98 7.65 7.97 7.87 0.19 100 1.17E−02 −0.50 1 hsa-asg-2459 3.87 2.983.94 3.60 0.54 0 1.51E−02 1.24 2 hsa-asg-2449 1.05 2.31 1.04 1.47 0.73 07.32E−03 −4.28 19 hsa-asg-2437 8.74 8.63 8.24 8.54 0.27 100 4.40E−020.43 1 hsa-asg-2433 3.00 3.09 3.75 3.28 0.41 0 2.12E−02 −0.84 2hsa-asg-2401 9.42 10.1 10.0 9.90 0.42 100 4.98E−02 0.55 1 hsa-asg-23713.00 4.32 4.85 4.06 0.95 67 9.41E−03 2.15 4 hsa-asg-2232 2.67 2.57 2.542.59 0.07 0 9.25E−03 0.60 2 hsa-asg-2076 2.02 2.02 1.91 1.98 0.06 04.73E−02 −0.82 2 hsa-asg-1978 5.16 4.17 5.18 4.84 0.58 67 1.71E−02 1.312 hsa-asg-1974 4.90 3.72 3.71 4.11 0.68 33 1.04E−02 1.34 3 hsa-asg-19376.67 3.48 5.21 5.12 1.60 67 1.45E−02 3.38 10 hsa-asg-1919 8.42 8.80 8.168.46 0.32 100 1.33E−02 −0.81 2 hsa-asg-1886 11.4 11.7 11.4 11.5 0.17 1002.02E−02 0.51 1 hsa-asg-1832 7.45 7.22 7.63 7.43 0.20 0 1.03E−02 −0.74 2hsa-asg-1807 4.94 2.22 5.11 4.09 1.62 67 2.53E−02 2.78 7 hsa-asg-179710.4 9.99 10.1 10.1 0.22 100 2.06E−02 0.40 1 hsa-asg-1726 10.4 10.6 10.510.5 0.14 100 1.78E−03 −0.52 1 hsa-asg-1658 4.42 4.53 5.06 4.67 0.34 01.70E−02 −0.84 2 hsa-asg-1557 2.84 2.71 2.85 2.80 0.08 0 2.86E−02 −0.922 hsa-asg-1532 7.18 7.07 6.34 6.86 0.46 0 1.28E−02 −1.24 2 hsa-asg-14510.4 10.8 10.6 10.6 0.22 100 7.73E−03 0.65 2 hsa-asg-1448 7.01 6.15 6.256.47 0.47 0 1.56E−02 −1.21 2 hsa-asg-14237 9.22 9.99 10.9 10.0 0.88 1004.80E−02 1.20 2 hsa-asg-142 8.94 8.97 8.90 8.94 0.04 100 4.00E−02 −0.411 hsa-asg-14197 13.7 14.2 13.7 13.8 0.32 100 4.76E−02 0.46 1hsa-asg-14189 14.8 14.7 14.7 14.7 0.03 100 1.70E−02 0.23 1 hsa-asg-141354.30 3.79 4.46 4.19 0.35 33 3.81E−02 0.67 2 hsa-asg-14053 7.29 7.54 6.957.26 0.30 33 7.49E−04 1.17 2 hsa-asg-14029 5.16 5.85 5.83 5.61 0.39 333.92E−02 −0.85 2 hsa-asg-14026 4.03 5.24 5.30 4.85 0.72 67 1.19E−02 1.753 hsa-asg-1398 5.20 5.07 5.67 5.31 0.32 100 4.77E−02 0.73 2hsa-asg-13973 7.05 7.43 7.32 7.27 0.19 33 3.77E−02 −0.79 2 hsa-asg-139637.81 6.94 7.91 7.55 0.53 0 3.34E−02 −0.93 2 hsa-asg-13895 4.68 4.81 4.464.65 0.18 100 5.76E−03 0.48 1 hsa-asg-13808 2.02 3.79 2.70 2.84 0.90 04.11E−02 −1.62 3 hsa-asg-13736 8.11 8.18 8.39 8.22 0.15 67 5.12E−03−0.50 1 hsa-asg-13705 6.07 4.42 5.83 5.44 0.89 100 3.46E−03 2.59 6hsa-asg-1369 2.48 4.92 4.31 3.90 1.27 33 3.92E−02 1.87 4 hsa-asg-1360611.6 11.3 11.5 11.4 0.14 100 2.77E−02 −0.41 1 hsa-asg-13604 10.3 10.410.6 10.4 0.18 100 1.98E−03 −0.63 2 hsa-asg-1360 8.46 8.87 8.21 8.520.34 67 3.84E−02 −0.45 1 hsa-asg-13501 5.94 5.30 4.31 5.18 0.82 334.04E−02 −1.43 3 hsa-asg-13334 5.32 5.84 5.88 5.68 0.31 67 3.41E−02 1.543 hsa-asg-13306 12.6 13.0 12.9 12.8 0.21 100 2.46E−02 0.50 1hsa-asg-13294 4.73 4.78 4.89 4.80 0.08 0 1.24E−02 0.38 1 hsa-asg-131975.29 4.95 4.51 4.92 0.39 100 1.05E−02 2.24 5 hsa-asg-13083 8.38 8.358.66 8.46 0.17 67 8.70E−03 −0.86 2 hsa-asg-12922 5.23 6.30 5.24 5.590.62 33 1.93E−02 −1.38 3 hsa-asg-1270 2.26 2.40 2.70 2.46 0.22 01.85E−02 −1.66 3 hsa-asg-12687 8.20 8.53 8.89 8.54 0.34 67 3.58E−02−0.57 1 hsa-asg-12602 3.87 4.92 3.79 4.19 0.63 33 5.43E−04 2.54 6hsa-asg-12556 10.2 9.80 10.1 10.0 0.22 100 3.37E−02 −0.55 1hsa-asg-12549 2.02 1.80 1.91 1.91 0.11 0 4.72E−02 −1.66 3 hsa-asg-125313.79 3.35 2.35 3.16 0.74 0 3.39E−02 −2.08 4 hsa-asg-12463 1.41 1.55 1.651.54 0.12 0 7.84E−03 −0.38 1 hsa-asg-12461 4.42 2.57 4.46 3.82 1.08 332.20E−02 2.09 4 hsa-asg-12460 7.94 7.62 7.79 7.78 0.16 100 4.19E−02−0.71 2 hsa-asg-12441 10.6 10.6 10.6 10.6 0.03 100 2.93E−02 −0.23 1hsa-asg-12421 9.40 8.62 9.45 9.16 0.46 100 4.86E−02 −0.70 2hsa-asg-12356 8.60 8.34 8.19 8.38 0.21 100 3.45E−03 −0.73 2hsa-asg-12344 9.13 8.77 8.66 8.85 0.24 100 8.73E−03 −0.89 2hsa-asg-12343 7.49 7.26 7.76 7.50 0.25 100 4.82E−02 0.76 2 hsa-asg-123203.70 3.72 5.53 4.32 1.05 33 4.08E−02 1.84 4 hsa-asg-12286 8.54 8.07 7.958.19 0.31 33 1.88E−02 −0.83 2 hsa-asg-12150 10.2 10.0 10.6 10.3 0.31 1003.55E−02 −0.54 1 hsa-asg-12045 5.85 5.43 6.27 5.85 0.42 67 1.59E−03 2.365 hsa-asg-12005 10.3 9.73 10.3 10.1 0.35 100 4.35E−02 0.62 2hsa-asg-11973 8.46 8.11 8.21 8.26 0.18 67 1.60E−02 −0.66 2 hsa-asg-1196911.0 9.95 9.11 10.0 0.97 100 4.27E−02 1.31 2 hsa-asg-11956 7.68 8.017.00 7.56 0.51 33 2.62E−02 −0.86 2 hsa-asg-11945 9.06 9.30 9.66 9.340.30 100 4.68E−02 −0.47 1 hsa-asg-11931 1.73 2.64 1.91 2.10 0.48 02.14E−02 −1.63 3 hsa-asg-11930 4.86 4.27 4.34 4.49 0.32 33 4.82E−02−0.80 2 hsa-asg-11898 6.65 6.70 6.68 6.68 0.03 100 5.38E−04 −1.12 2hsa-asg-1189 7.52 7.22 7.14 7.29 0.20 33 4.51E−02 −0.97 2 hsa-asg-118775.41 5.94 5.08 5.48 0.44 0 3.86E−03 −1.30 2 hsa-asg-11797 7.80 7.85 7.447.70 0.23 33 4.96E−02 −0.43 1 hsa-asg-1174 9.53 9.21 9.07 9.27 0.24 1002.22E−02 −0.45 1 hsa-asg-11643 3.60 4.42 3.71 3.91 0.44 0 1.46E−02 0.932 hsa-asg-11623 11.0 11.0 10.8 11.0 0.12 0 1.19E−02 −0.44 1hsa-asg-11617 9.79 9.71 9.99 9.83 0.14 100 1.96E−02 −0.53 1hsa-asg-11612 8.58 9.37 8.89 8.94 0.40 67 3.25E−03 1.04 2 hsa-asg-1160612.0 12.0 11.8 11.9 0.13 100 3.67E−03 0.65 2 hsa-asg-11580 3.00 4.113.12 3.41 0.61 33 2.69E−02 1.36 3 hsa-asg-11555 3.60 3.79 3.63 3.67 0.100 2.13E−02 −0.85 2 hsa-asg-11521 8.29 8.72 8.70 8.57 0.24 67 4.85E−02−0.68 2 hsa-asg-11513 9.15 8.81 9.15 9.03 0.20 100 3.88E−02 −0.36 1hsa-asg-11193 11.7 11.7 11.9 11.8 0.08 67 3.30E−02 −0.31 1 hsa-asg-111284.24 4.63 4.65 4.50 0.23 67 4.37E−02 0.50 1 hsa-asg-11112 11.2 10.9 11.011.0 0.16 100 3.01E−02 −0.33 1 hsa-asg-11002 1.73 0.20 1.65 1.20 0.86 01.34E−02 −2.03 4 hsa-asg-10958 2.67 3.65 3.12 3.15 0.49 0 4.08E−02 0.962 hsa-asg-10901 5.88 6.55 5.06 5.83 0.75 67 2.29E−02 −1.22 2hsa-asg-10810 6.61 5.01 4.36 5.33 1.16 100 2.40E−02 1.84 4 hsa-asg-10721.41 2.02 1.36 1.60 0.37 0 4.29E−02 −1.39 3 hsa-asg-10639 11.6 11.3 11.211.3 0.22 100 3.73E−02 −0.37 1 hsa-asg-10517 3.14 3.90 3.94 3.66 0.45 08.06E−03 1.65 3 hsa-asg-105 6.93 4.44 5.09 5.49 1.29 33 3.44E−02 2.21 5hsa-asg-10487 2.02 1.41 1.04 1.49 0.49 0 1.24E−02 −3.09 8 hsa-asg-104862.26 2.02 1.91 2.07 0.18 0 1.73E−02 −0.84 2 hsa-asg-10464 2.26 2.02 1.912.07 0.18 0 4.22E−02 −2.04 4 hsa-asg-10392 9.35 9.48 9.38 9.40 0.07 1001.12E−02 −0.71 2 hsa-asg-10280 5.70 5.66 6.21 5.86 0.31 100 1.68E−032.74 7 hsa-asg-10278 3.14 2.64 3.12 2.97 0.28 0 2.36E−02 0.60 2hsa-asg-1026 4.63 3.72 5.17 4.51 0.73 33 2.55E−02 −1.15 2 hsa-asg-102473.95 4.55 4.01 4.17 0.33 33 1.78E−04 2.02 4 hsa-asg-1020 7.69 8.20 8.148.01 0.28 67 9.20E−03 −0.65 2 hsa-asg-1018 3.70 4.65 5.71 4.68 1.00 01.40E−02 −1.92 4 hsa-asg-10131 5.98 4.71 4.65 5.11 0.75 100 2.42E−021.52 3 hsa-miR-224 6.68 9.06 10.8 8.87 2.09 100 2.05E−02 3.57 12hsa-miR-99b 9.40 10.2 9.37 9.66 0.47 0 6.19E−03 1.21 2 LNpos2/LNneg2,LNpos3/LNneg3, and LNpos4/LNneg4 represent matched samples obtained fromthree different patients. SD, standard deviation; %, percentage ofsamples expressing miRNA above background levels; Log2Diff, differencein VSN-transformed expression between LNpos and LNneg samples.

Example 41 miRNAs Differentially Expressed Between Normal Breast Tissueand Cancer-negative Axillary Lymph Nodes from Breast Cancer Patients

To identify the miRNAs differentially expressed between normal breasttissue samples and cancer negative axillary lymph nodes, the inventorscompared the average miRNA expression levels in three normal humanbreast tissue samples (described above in Example 19) with those in thefour cancer-negative axillary lymph nodes from ductal breast cancerpatients (described above in Example 40). RNA labeling and microarrayexpression analysis was performed as described above in Example 40. Atotal of 620 miRNAs (168 verified and 452 predicted) were significantlydifferentially expressed between normal breast (NBr) and negative lymphnode (LNneg) samples (Log2Diff(NBr vs LNneg)≧1; t-test (NBr vsLNneg)<0.05) (Table 40). Among the verified miRNAs, only three(hsa-miR-155, -150, and -155) had average expression levels that werelower (3-6 fold) in NBr compared to LNneg. One (hsa-miR-205) had anaverage expression level at least 1,000 fold higher in NBr compared tonegative LNneg; seven (hsa-miR-200b, -368, -200a, -141, -199b, -411, and-335) had average expression levels 100- to 500-fold higher in NBr;eleven (hsa-miR-1, -224, -218, -200c, -551b, -19a, -140, -495, -182,-424, and -451) had average expression levels 50- to 95-fold higher inNBr samples; nineteen (hsa-miR-452, -486, -429, -189, -96, -421-3p,-133a, -30a-3p, -487b, -452, -154, -375, -127, -22, -148b, -379, -19b,-489, and -152) had average expression levels 20- to 49-fold higher inNBr; and 98 had expression levels 5- to 19-fold higher in NBr samplescompared to LNneg (Table 40). Among the predicted miRNAs, three(hsa-asg-5021_st1, -13254_st1, and hsa-miR154_st1) had averageexpression levels more than 50-fold lower in LNneg compared to NBrsamples, and 24 had average expression levels 10- to 50-fold lower inLNneg compared to NBr samples (Table 40).

TABLE 40 miRNAs differentially expressed between three normal breast(NBr) tissue samples and four cancer-negative lymph nodes (LNneg1-4)obtained from breast cancer patients. Micro Probe NBr LNneg RNA ID 1 2 3Mean SD % 1 2 hsa-let-7a hsa-let-7a_st2 14.97 14.67 14.62 14.75 0.19 10012.99 12.58 hsa-let-7b hsa-let-7b_st2 13.68 13.46 13.50 13.55 0.11 10011.22 10.72 hsa-let-7c hsa-let-7c_st2 14.54 14.25 14.32 14.37 0.15 10012.11 11.99 hsa-let-7d hsa-let-7d_st2 14.54 14.24 14.34 14.37 0.15 10012.27 12.03 hsa-let-7e hsa-let-7e_st1 12.44 12.34 12.56 12.45 0.11 10010.22 9.61 hsa-let-7f hsa-let-7f_st1 14.57 14.27 14.37 14.40 0.15 10012.02 11.76 hsa-let-7g hsa-let-7g_st2 14.00 13.58 13.80 13.79 0.21 10012.20 11.41 hsa-let-7i hsa-let-7i_st1 13.55 13.10 13.36 13.34 0.22 10011.08 10.41 hsa-miR-1 hsa-miR-1_st1 6.42 12.28 8.34 9.02 2.99 100 2.081.35 hsa-miR-100 hsa-miR-100_st2 13.26 12.89 12.96 13.04 0.20 100 9.419.53 hsa-miR-101 hsa-miR-101_st1 8.96 8.67 9.64 9.09 0.50 100 5.69 6.20hsa-miR-103 hsa-miR-103_st1 13.44 13.23 13.36 13.34 0.11 100 10.78 10.79hsa-miR-106a hsa-miR-106a_st1 13.04 12.32 12.66 12.67 0.36 100 9.68 9.37hsa-miR-106b hsa-miR-106b_st2 11.61 10.58 11.01 11.07 0.52 100 7.39 6.69hsa-miR-107 hsa-miR-107_st2 13.24 13.07 13.22 13.17 0.09 100 10.29 10.58hsa-miR-10a hsa-miR-10a_st1 11.74 11.83 11.81 11.79 0.05 100 8.20 8.14hsa-miR-10b hsa-miR-10b_st2 12.96 12.83 12.84 12.88 0.07 100 9.46 10.02hsa-miR-125a hsa-miR-125a_st2 12.40 12.10 12.45 12.32 0.19 100 9.87 9.93hsa-miR-125b hsa-miR-125b_st1 14.60 14.16 14.20 14.32 0.24 100 10.6911.56 hsa-miR-126 hsa-miR-126_st2 14.61 14.60 14.47 14.56 0.08 100 12.3411.78 hsa-miR-126* hsa-miR-126_st2 4.99 5.51 6.37 5.62 0.70 100 2.512.76 hsa-miR-127 hsa-miR-127_st1 10.43 8.06 9.23 9.24 1.18 100 4.68 4.27hsa-miR-128a hsa-miR-128a_st2 9.63 9.63 9.15 9.47 0.28 100 5.28 5.95hsa-miR-128b hsa-miR-128b_st1 9.71 9.83 9.81 9.78 0.07 100 5.99 7.01hsa-miR-130a hsa-miR-130a_st1 11.78 10.85 11.16 11.26 0.47 100 8.01 8.35hsa-miR-130b hsa-miR-130b_st1 8.20 7.48 7.98 7.89 0.37 100 5.88 6.53hsa-miR-132 hsa-miR-132_st1 10.13 8.20 8.90 9.07 0.98 33.33 2.20 6.16hsa-miR-133a hsa-miR-133a_st1 5.22 11.48 4.77 7.16 3.75 33.33 2.69 1.48hsa-miR-134 hsa-miR-134_st2 8.84 8.26 8.06 8.38 0.41 100 7.40 7.97hsa-miR-137 hsa-miR-137_st1 2.38 2.49 2.12 2.33 0.19 0 2.08 2.08hsa-miR-139 hsa-miR-139_st2 10.50 10.45 10.60 10.52 0.08 100 7.60 4.95hsa-miR-140 hsa-miR-140_st2 7.33 7.49 8.28 7.70 0.51 100 2.69 1.35hsa-miR-141 hsa-miR-141_st1 11.26 7.11 9.31 9.23 2.08 100 1.83 1.74hsa-miR-143 hsa-miR-143_st2 14.18 14.09 14.07 14.11 0.06 100 9.93 9.84hsa-miR-145 hsa-miR-145_st1 14.67 14.51 14.48 14.55 0.10 100 12.23 12.28hsa-miR-146b hsa-miR-146b_st1 12.27 10.47 11.75 11.49 0.93 100 9.47 9.10hsa-miR-148a hsa-miR-148a_st1 12.93 11.80 12.38 12.37 0.57 100 8.68 7.61hsa-miR-148b hsa-miR-148b_st2 9.78 9.70 9.82 9.76 0.06 100 4.91 4.53hsa-miR-150 hsa-miR-150_st1 12.01 12.18 12.16 12.12 0.10 100 13.80 13.42hsa-miR-151 hsa-miR-151_st2 10.03 9.45 9.63 9.70 0.30 100 6.77 6.09hsa-miR-152 hsa-miR-152_st1 11.81 11.37 11.73 11.64 0.24 100 7.06 6.96hsa-miR-154 hsa-miR-154_st2 8.25 5.85 6.72 6.94 1.22 66.66 2.99 1.74hsa-miR-154* hsa-miR-154_st2 5.42 5.75 6.08 5.75 0.33 100 3.25 2.08hsa-miR-155 hsa-miR-155_st1 9.03 9.17 9.32 9.17 0.15 100 10.79 10.00hsa-miR-15a hsa-miR-15a_st1 12.12 11.79 12.32 12.08 0.27 100 8.81 8.10hsa-miR-15b hsa-miR-15b_st1 11.46 11.44 11.94 11.61 0.28 100 9.15 8.36hsa-miR-16 hsa-miR-16_st2 14.41 14.45 14.37 14.41 0.04 100 12.13 12.04hsa-miR-17- hsa-miR-17- 8.98 6.99 7.55 7.84 1.03 100 6.67 4.67 3p 3p_st1hsa-miR-17- hsa-miR-17- 12.85 11.83 12.43 12.37 0.51 100 9.52 9.17 5p5p_st1 hsa-miR-181a hsa-miR-181a_st1 11.83 10.83 10.96 11.21 0.54 1009.13 9.33 hsa-miR- hsa-miR-213_st1 7.04 7.04 7.42 7.16 0.22 100 2.515.30 181a* hsa-miR-181b hsa-miR-181b_st2 9.19 8.25 8.67 8.70 0.47 1007.45 7.38 hsa-miR-181d hsa-miR-181d_st1 7.21 6.91 7.77 7.29 0.44 1004.18 4.73 hsa-miR-182 hsa-miR-182_st1 9.70 9.09 9.63 9.47 0.33 100 4.123.75 hsa-miR-185 hsa-miR-185_st2 9.58 9.81 10.80 10.06 0.65 100 5.066.84 hsa-miR-186 hsa-miR-186_st1 6.02 5.81 7.06 6.30 0.67 100 3.57 1.86hsa-miR-189 hsa-miR-189_st1 6.18 7.04 5.84 6.35 0.62 66.66 1.54 0.58hsa-miR-18a hsa-miR-18a_st1 7.31 5.05 6.94 6.43 1.21 100 2.51 2.08hsa-miR-18b hsa-miR-18b_st1 6.63 5.09 6.50 6.07 0.85 100 3.36 1.62hsa-miR-191 hsa-miR-191_st2 13.23 12.86 13.06 13.05 0.19 100 11.18 11.02hsa-miR-192 hsa-miR-192_st2 7.96 7.76 8.56 8.09 0.42 100 6.92 5.76hsa-miR-193a hsa-miR-193a_st1 9.64 9.17 9.17 9.33 0.27 100 6.03 6.23hsa-miR-193b hsa-miR-193b_st1 11.05 12.17 12.16 11.79 0.65 100 8.14 7.05hsa-miR-194 hsa-miR-194_st2 8.68 7.84 8.82 8.45 0.53 100 5.69 5.23hsa-miR-195 hsa-miR-195_st2 14.32 13.82 13.93 14.03 0.26 100 10.38 9.75hsa-miR-196b hsa-miR-196b_st2 7.13 3.76 7.05 5.98 1.92 66.66 3.91 2.45hsa-miR-199a hsa-miR-199a_st2 12.99 12.28 12.43 12.57 0.37 100 9.70 9.17hsa-miR- hsa-miR-199a-_st1 14.39 13.95 13.99 14.11 0.24 100 10.66 9.33199a* hsa-miR-199b hsa-miR-199b_st2 12.23 11.44 11.87 11.85 0.40 1003.25 5.52 hsa-miR-19a hsa-miR-19a_st1 9.37 8.45 9.10 8.97 0.47 100 3.572.08 hsa-miR-19b hsa-miR-19b_st2 12.95 12.11 12.57 12.54 0.42 100 8.007.31 hsa-miR-200a hsa-miR-200a_st2 11.71 8.54 9.48 9.91 1.63 100 2.082.45 hsa-miR-200b hsa-miR-200b_st1 12.95 10.21 11.57 11.57 1.37 100 2.511.86 hsa-miR-200c hsa-miR-200c_st1 13.23 10.88 11.90 12.00 1.18 100 5.065.86 hsa-miR-203 hsa-miR-203_st2 7.43 6.28 7.83 7.18 0.81 100 3.36 3.34hsa-miR-205 hsa-miR-205_st1 14.02 13.00 13.39 13.47 0.52 100 0.86 2.69hsa-miR-20a hsa-miR-20a_st2 12.66 11.83 12.24 12.24 0.41 100 9.19 8.56hsa-miR-20b hsa-miR-20b_st2 10.76 9.88 10.77 10.47 0.51 100 7.39 6.98hsa-miR-21 hsa-miR-21_st2 13.82 13.64 13.88 13.78 0.12 100 10.80 9.74hsa-miR-214 hsa-miR-214_st1 10.39 10.05 9.96 10.14 0.23 100 8.49 9.41hsa-miR-215 hsa-miR-215_st1 5.58 6.18 7.31 6.36 0.88 100 2.51 2.28hsa-miR-218 hsa-miR-218_st2 9.00 8.32 8.64 8.66 0.34 100 4.05 1.35hsa-miR-22 hsa-miR-22_st1 13.32 13.56 13.74 13.54 0.21 100 8.89 9.06hsa-miR-221 hsa-miR-221_st1 12.86 12.24 12.48 12.53 0.31 100 10.19 9.55hsa-miR-222 hsa-miR-222_st2 12.51 11.97 11.95 12.14 0.31 100 10.65 9.93hsa-miR-223 hsa-miR-223_st1 11.40 11.75 12.48 11.88 0.55 100 9.37 9.34hsa-miR-224 hsa-miR-224_st2 11.33 12.08 11.84 11.75 0.38 100 5.45 5.33hsa-miR-23a hsa-miR-23a_st2 14.58 14.50 14.51 14.53 0.04 100 11.86 11.73hsa-miR-23b hsa-miR-23b_st1 14.29 14.24 14.27 14.26 0.03 100 11.64 11.44hsa-miR-24 hsa-miR-24_st1 13.65 13.60 13.61 13.62 0.03 100 10.23 10.14hsa-miR-25 hsa-miR-25_st2 12.32 12.28 12.40 12.33 0.06 100 9.69 9.79hsa-miR-26a hsa-miR-26a_st2 14.82 14.64 14.49 14.65 0.17 100 13.06 12.62hsa-miR-26b hsa-miR-26b_st1 12.88 12.61 13.18 12.89 0.29 100 10.06 9.20hsa-miR-27a hsa-miR-27a_st2 12.38 12.89 13.00 12.76 0.33 100 8.41 8.64hsa-miR-27b hsa-miR-27b_st1 12.65 12.83 12.76 12.75 0.09 100 8.08 8.11hsa-miR-28 hsa-miR-28_st1 11.32 10.88 11.12 11.11 0.22 100 8.45 7.82hsa-miR-299- hsa-miR-299- 5.97 5.83 6.88 6.23 0.57 100 2.51 2.08 5p5p_st2 hsa-miR-29a hsa-miR-29a_st2 13.48 13.26 13.38 13.37 0.11 10011.97 11.45 hsa-miR-29b hsa-miR-29b_st1 11.69 11.17 11.96 11.61 0.40 1009.79 8.32 hsa-miR-29c hsa-miR-29c_st2 10.51 10.54 11.30 10.78 0.45 1009.31 7.57 hsa-miR-30a- hsa-miR-30a- 10.41 10.65 10.55 10.54 0.12 1004.87 5.94 3p 3p_st1 hsa-miR-30a- hsa-miR-30a- 13.65 13.10 13.19 13.310.29 100 9.61 9.62 5p 5p_st2 hsa-miR-30b hsa-miR-30b_st2 13.07 12.4712.91 12.81 0.31 100 9.69 9.02 hsa-miR-30c hsa-miR-30c_st1 13.75 13.3313.51 13.53 0.21 100 10.75 10.18 hsa-miR-30d hsa-miR-30d_st1 13.33 12.7413.08 13.05 0.29 100 10.10 10.30 hsa-miR-30e- hsa-miR-30e- 9.06 9.779.61 9.48 0.37 100 7.65 6.41 3p 3p_st1 hsa-miR-30e- hsa-miR-30e- 12.1411.39 11.67 11.73 0.38 100 8.61 7.64 5p 5p_st2 hsa-miR-31 hsa-miR-31_st210.75 8.14 9.29 9.39 1.31 100 5.67 6.96 hsa-miR-320 hsa-miR-320_st110.69 10.78 10.67 10.71 0.06 100 9.75 9.60 hsa-miR-329 hsa-miR-329_st17.54 5.43 6.75 6.57 1.07 100 2.99 3.24 hsa-miR-335 hsa-miR-335_st1 13.0312.83 13.23 13.03 0.20 100 6.37 5.28 hsa-miR-34a hsa-miR-34a_st1 9.338.74 8.60 8.89 0.39 100 5.90 5.96 hsa-miR-34b hsa-miR-34b_st2 6.06 2.996.13 5.06 1.79 66.66 2.69 2.08 hsa-miR-361 hsa-miR-361_st2 11.47 11.0511.33 11.29 0.21 100 8.58 8.30 hsa-miR-362 hsa-miR-362_st1 7.72 7.337.18 7.41 0.28 100 5.82 6.00 hsa-miR-363 hsa-miR-363_st2 8.31 9.13 9.719.05 0.70 100 6.24 7.00 hsa-miR-365 hsa-miR-365_st1 6.06 7.13 7.92 7.040.93 100 2.99 2.89 hsa-miR-368 hsa-miR-368_st2 10.88 9.03 10.47 10.130.97 100 1.54 1.35 hsa-miR-369- hsa-miR-369- 8.27 5.63 7.34 7.08 1.34100 1.83 1.62 5p 5p_st2 hsa-miR-375 hsa-miR-375_st1 7.02 6.36 6.76 6.710.33 0 1.38 1.48 hsa-miR-376a hsa-miR-376a_st2 8.33 5.25 7.10 6.90 1.55100 2.85 2.28 hsa-miR-377 hsa-miR-377_st1 6.95 4.94 7.42 6.44 1.32 1001.83 1.86 hsa-miR-379 hsa-miR-379_st1 9.59 8.33 8.93 8.95 0.63 100 2.994.05 hsa-miR-381 hsa-miR-381_st2 7.94 5.40 6.69 6.68 1.27 66.66 4.182.37 hsa-miR-382 hsa-miR-382_st1 9.50 7.98 8.25 8.58 0.81 100 5.57 4.55hsa-miR-409- hsa-miR-409- 6.93 4.98 7.01 6.31 1.15 66.66 3.75 2.37 5p5p_st1 hsa-miR-410 hsa-miR-410_st1 6.26 3.13 5.92 5.11 1.72 66.66 2.313.24 hsa-miR-411 hsa-miR-411_st1 9.25 8.07 8.21 8.51 0.64 100 0.86 2.37hsa-miR-421 hsa-miR-421- 7.00 7.36 8.22 7.53 0.63 100 3.13 1.86 3p_st1hsa-miR-422a hsa-miR-422a_st1 10.90 11.75 11.25 11.30 0.42 100 6.93 6.31hsa-miR-422b hsa-miR-422b_st2 12.13 12.79 11.99 12.30 0.43 100 8.48 8.85hsa-miR-423 hsa-miR-423_st1 10.02 9.43 9.52 9.66 0.31 100 7.07 7.76hsa-miR-424 hsa-miR-424_st1 9.13 6.45 8.54 8.04 1.41 100 1.83 1.62hsa-miR-425- hsa-asg-14166_st2 10.37 10.38 10.61 10.46 0.13 100 8.428.16 5p hsa-miR-429 hsa-miR-429_st1 8.53 7.14 8.50 8.05 0.79 100 1.833.60 hsa-miR-432 hsa-miR-432_st2 6.93 6.06 7.04 6.68 0.54 100 3.66 4.05hsa-miR-451 hsa-miR-451_st1 13.45 13.23 13.92 13.53 0.35 100 7.73 8.54hsa-miR-452 hsa-miR-452_st1 10.40 10.61 10.60 10.53 0.12 100 4.18 5.62hsa-miR-452* hsa-miR-452-_st1 7.47 7.61 7.62 7.56 0.08 100 2.85 0.74hsa-miR-486 hsa-miR-486_st2 10.51 11.75 11.69 11.32 0.70 100 5.26 6.46hsa-miR-487a hsa-asg-6915_st1 7.92 6.50 7.12 7.18 0.71 100 5.60 4.91hsa-miR-487b hsa-miR-487b_st2 10.01 7.76 8.93 8.90 1.13 100 4.66 4.19hsa-miR-489 hsa-miR-489_st2 5.64 7.10 7.52 6.75 0.99 100 3.13 1.62hsa-miR-493- hsa-miR-493- 5.83 2.84 5.99 4.89 1.77 66.66 1.54 1.86 5p5p_st1 hsa-miR-495 hsa-miR-495_st1 9.05 7.23 8.32 8.20 0.91 100 2.692.28 hsa-miR-497 hsa-miR-497_st1 12.82 11.98 12.29 12.36 0.42 100 7.887.99 hsa-miR-500 hsa-miR-500_st1 8.23 7.89 7.97 8.03 0.18 100 7.11 7.14hsa-miR-503 hsa-miR141_st1 8.87 7.69 8.46 8.34 0.60 66.66 5.61 6.69hsa-miR-505 hsa-miR-505_st1 6.58 7.02 7.42 7.01 0.42 100 4.05 4.42hsa-miR-516- hsa-miR-516- 6.63 5.02 4.44 5.36 1.13 33.33 1.83 2.08 5p5p_st1 hsa-miR-517a hsa-miR-517a_st1 6.47 6.38 6.78 6.54 0.21 100 2.853.89 hsa-miR-542- hsa-miR-542- 7.36 4.82 5.64 5.94 1.30 100 4.52 2.76 3p3p_st1 hsa-miR-542- hsa-miR-542- 6.38 5.02 5.35 5.58 0.71 33.33 1.832.69 5p 5p_st2 hsa-miR-551b hsa-miR-551b_st1 7.58 8.21 8.92 8.24 0.67100 1.54 2.18 hsa-miR-557 hsa-miR-557_st1 9.23 9.95 9.51 9.56 0.37 1008.65 8.44 hsa-miR-565 hsa-miR-565_st1 11.20 12.02 11.63 11.62 0.41 10010.27 10.17 hsa-miR-566 hsa-miR-566_st1 6.27 5.66 7.29 6.41 0.83 0 9.409.08 hsa-miR-574 hsa-miR-574_st1 9.64 9.20 9.57 9.47 0.23 100 7.53 7.06hsa-miR-583 hsa-miR-583_st1 6.99 6.41 7.00 6.80 0.34 100 2.85 2.76hsa-miR-598 hsa-miR-598_st2 6.67 5.43 5.72 5.94 0.65 100 2.08 3.60hsa-miR-624 hsa-miR-624_st2 4.85 3.48 5.96 4.76 1.24 66.66 3.13 2.45hsa-miR-628 hsa-miR-628_st2 8.36 8.10 8.85 8.44 0.38 100 7.06 7.95hsa-miR-630 hsa-miR-630_st1 4.69 4.07 5.16 4.64 0.55 66.66 3.25 2.89hsa-miR-655 hsa-miR-655_st2 5.80 5.83 6.02 5.88 0.12 100 3.13 3.13hsa-miR-660 hsa-miR-660_st1 9.49 8.95 9.54 9.33 0.33 100 6.78 6.45hsa-miR-9* hsa-miR-9-_st2 5.20 3.68 5.47 4.78 0.97 66.66 2.51 3.52hsa-miR-92 hsa-miR-92_st1 12.48 11.82 12.02 12.11 0.34 100 11.21 11.04hsa-miR-93 hsa-miR-93_st2 11.12 10.65 11.02 10.93 0.25 100 7.78 8.07hsa-miR-96 hsa-miR-96_st1 7.93 6.61 7.48 7.34 0.67 100 2.08 2.28hsa-miR-98 hsa-miR-98_st1 10.17 9.90 11.00 10.36 0.57 100 6.21 5.76hsa-miR-99a hsa-miR-99a_st2 13.81 13.33 13.59 13.58 0.24 100 9.44 9.42hsa-miR-99b hsa-miR-99b_st1 11.39 11.21 11.42 11.34 0.12 66.66 8.04 8.22hsa-miR154 hsa-miR154_st1 9.37 9.79 10.52 9.89 0.58 100 3.57 3.43hsa-miR144 hsa-miR144_st2 8.26 7.44 7.91 7.87 0.41 100 1.83 1.86hsa-asg-9769 hsa-asg-9769_st2 5.31 4.78 5.40 5.16 0.34 66.66 2.99 2.45hsa-asg-9483 hsa-asg-9483_st1 9.93 10.78 10.42 10.38 0.43 100 8.18 8.15hsa-asg-9405 hsa-asg-9405_st1 10.71 11.81 10.90 11.14 0.59 100 7.78 8.41hsa-asg-9253 hsa-asg-9253_st2 2.72 2.05 2.80 2.52 0.41 0 7.20 7.06hsa-asg-9232 hsa-asg-9232_st2 5.59 5.28 4.69 5.19 0.46 33.33 2.31 2.18hsa-asg-9200 hsa-asg-9200_st1 6.30 5.93 5.60 5.94 0.35 100 4.05 3.96hsa-asg-9112 hsa-asg-9112_st2 12.45 12.82 12.58 12.62 0.19 100 9.9410.04 hsa-asg-8901 hsa-asg-8901_st2 13.45 14.00 13.44 13.63 0.32 10012.30 12.23 hsa-asg-860 hsa-asg-860_st2 4.74 4.21 4.34 4.43 0.28 66.662.99 2.61 hsa-asg-8195 hsa-asg-8195_st2 0.52 1.79 1.37 1.23 0.65 0 8.276.86 hsa-asg-7985 hsa-asg-7985_st2 11.73 11.91 11.74 11.79 0.11 10010.50 10.69 hsa-asg-772 hsa-asg-772_st1 10.13 9.69 9.88 9.90 0.22 1008.93 8.65 hsa-asg-7569 hsa-asg-7569_st1 6.70 6.18 6.91 6.60 0.38 1008.59 8.16 hsa-asg-7461 hsa-asg-7461_st2 8.40 8.38 8.67 8.49 0.16 1006.81 7.17 hsa-asg-7352 hsa-asg-7352_st1 11.19 11.54 11.09 11.27 0.23 1009.82 9.82 hsa-asg-7189 hsa-asg-7189_st1 11.80 12.20 11.68 11.89 0.27 10010.08 10.02 hsa-asg-7098 hsa-asg-7098_st2 0.97 2.05 1.90 1.64 0.58 07.19 6.69 hsa-asg-6957 hsa-asg-6957_st1 11.54 11.83 11.58 11.65 0.16 1009.90 10.05 hsa-asg-6932 hsa-asg-6932_st1 10.04 10.22 10.31 10.19 0.14100 11.33 11.85 hsa-asg-6870 hsa-asg-6870_st1 11.52 12.13 11.65 11.760.32 100 10.37 10.61 hsa-asg-680 hsa-asg-680_st2 10.96 10.57 10.67 10.730.20 100 7.50 7.25 hsa-asg-6457 hsa-asg-6457_st2 5.16 4.82 5.52 5.170.35 66.66 3.36 3.43 hsa-asg-6247 hsa-asg-6247_st1 11.41 11.83 11.5711.60 0.21 100 9.33 10.21 hsa-asg-5998 hsa-asg-5998_st1 12.95 13.3812.94 13.09 0.25 100 9.50 9.14 hsa-asg-5932 hsa-asg-5932_st2 14.65 14.5814.33 14.52 0.17 100 13.15 13.07 hsa-asg-5760 hsa-asg-5760_st1 1.78 1.482.12 1.79 0.32 0 7.21 5.52 hsa-asg-5021 hsa-asg-5021_st1 9.33 8.06 8.688.69 0.63 100 2.08 2.61 hsa-asg-4557 hsa-asg-4557_st2 6.80 6.45 7.757.00 0.67 100 3.25 3.60 hsa-asg-4523 hsa-asg-4523_st2 8.58 8.75 9.068.80 0.24 100 7.22 7.53 hsa-asg-4515 hsa-asg-4515_st1 4.74 4.39 5.014.71 0.31 66.66 2.31 2.08 hsa-asg-4416 hsa-asg-4416_st1 4.08 3.85 4.394.10 0.27 33.33 1.83 2.28 hsa-asg-4350 hsa-asg-4350_st1 2.10 2.05 1.071.74 0.58 0 6.11 5.72 hsa-asg-4163 hsa-asg-4163_st2 5.42 5.22 5.19 5.280.12 100 3.47 3.52 hsa-asg-3998 hsa-asg-3998_st1 10.96 11.63 11.04 11.210.37 100 8.72 8.11 hsa-asg-3811 hsa-asg-3811_st1 10.50 9.73 10.47 10.230.43 100 7.54 7.78 hsa-asg-3604 hsa-asg-3604_st1 5.42 5.53 5.56 5.500.07 100 3.25 3.43 hsa-asg-3545 hsa-asg-3545_st1 12.35 12.92 12.71 12.660.29 100 11.08 11.06 hsa-asg-3167 hsa-asg-3167_st1 11.18 11.62 11.2011.33 0.25 100 10.03 10.46 hsa-asg-3138 hsa-asg-3138_st2 10.03 10.209.96 10.06 0.13 100 8.57 7.95 hsa-asg-2944 hsa-asg-2944_st2 4.94 4.695.25 4.96 0.28 66.66 3.13 2.76 hsa-asg-2824 hsa-asg-2824_st2 10.09 9.589.80 9.82 0.25 100 11.24 11.16 hsa-asg-2785 hsa-asg-2785_st2 12.53 12.8612.60 12.66 0.17 100 10.07 9.83 hsa-asg-2750 hsa-asg-2750_st2 13.0613.59 13.25 13.30 0.27 100 12.15 12.06 hsa-asg-2739 hsa-asg-2739_st211.51 11.56 11.59 11.55 0.04 100 10.25 10.50 hsa-asg-253 hsa-asg-253_st210.62 10.66 10.91 10.73 0.16 100 9.25 9.00 hsa-asg-2445 hsa-asg-2445_st11.40 1.14 2.80 1.78 0.89 0 7.58 6.32 hsa-asg-2073 hsa-asg-2073_st1 4.995.09 4.65 4.91 0.23 100 2.69 3.34 hsa-asg-1895 hsa-asg-1895_st1 11.9012.35 12.07 12.11 0.23 100 9.77 10.32 hsa-asg-1514 hsa-asg-1514_st1 8.508.56 9.20 8.75 0.39 66.66 7.01 6.78 hsa-asg-1468 hsa-asg-1468_st2 10.5410.81 10.28 10.54 0.26 100 8.93 9.35 hsa-asg-14238 hsa-asg-14238_st17.28 8.04 8.32 7.88 0.54 100 3.98 3.02 hsa-asg-14204 hsa-asg-14204_st17.77 7.41 7.71 7.63 0.19 0 8.61 9.00 hsa-asg-14186 hsa-asg-14186_st15.04 5.22 4.18 4.81 0.56 66.66 2.08 2.89 hsa-asg-14175 hsa-asg-14175_st16.71 5.19 6.89 6.26 0.94 100 2.08 2.45 hsa-asg-14170 hsa-asg-14170_st18.43 7.96 8.80 8.39 0.42 100 4.05 2.76 hsa-asg-14146 hsa-asg-14146_st17.31 7.20 6.64 7.05 0.36 100 2.31 1.35 hsa-asg-14134 hsa-asg-14134_st18.50 8.53 9.30 8.78 0.46 100 4.36 5.06 hsa-asg-14038 hsa-asg-14038_st111.72 12.67 11.93 12.11 0.50 100 9.86 9.77 hsa-asg-13770hsa-asg-13770_st1 9.71 9.98 9.78 9.82 0.14 100 8.10 8.15 hsa-asg-13403hsa-asg-13403_st2 9.92 10.32 10.16 10.13 0.20 100 7.81 6.98hsa-asg-13354 hsa-asg-13354_st1 11.86 11.65 11.11 11.54 0.38 100 13.8913.61 hsa-asg-13312 hsa-asg-13312_st2 5.12 5.94 4.34 5.13 0.80 0 10.579.78 hsa-asg-13258 hsa-asg-13258_st2 9.08 8.75 9.47 9.10 0.36 100 4.525.79 hsa-asg-13254 hsa-asg-13254_st1 9.42 8.19 8.42 8.67 0.65 100 1.221.74 hsa-asg-13244 hsa-asg-13244_st1 7.74 7.69 7.50 7.64 0.13 100 9.729.15 hsa-asg-13205 hsa-asg-13205_st1 7.97 8.87 8.92 8.59 0.53 100 2.314.05 hsa-asg-13127 hsa-asg-13127_st2 7.91 7.26 7.17 7.45 0.41 100 4.365.24 hsa-asg-12949 hsa-asg-12949_st1 9.15 9.71 9.27 9.37 0.29 100 7.868.12 hsa-asg-12902 hsa-asg-12902_st1 8.22 7.43 7.37 7.67 0.48 100 9.639.46 hsa-asg-12885 hsa-asg-12885_st1 4.94 4.86 4.95 4.92 0.05 100 3.132.76 hsa-asg-12765 hsa-asg-12765_st2 5.35 4.94 5.22 5.17 0.21 100 2.691.62 hsa-asg-12729 hsa-asg-12729_st1 13.40 13.73 13.33 13.49 0.21 10011.43 11.17 hsa-asg-12600 hsa-asg-12600_st2 9.42 9.75 9.38 9.52 0.20 1008.59 8.54 hsa-asg-12471 hsa-asg-12471_st1 3.58 3.58 3.64 3.60 0.04 1002.31 1.86 hsa-asg-12423 hsa-asg-12423_st1 9.06 9.38 9.54 9.32 0.24 08.31 8.04 hsa-asg-12406 hsa-asg-12406_st1 11.32 12.10 11.36 11.59 0.44100 9.09 9.21 hsa-asg-12372 hsa-asg-12372_st2 12.13 12.41 12.20 12.250.14 100 10.40 10.36 hsa-asg-12192 hsa-asg-12192_st2 9.42 9.83 9.68 9.640.21 100 8.15 8.50 hsa-asg-11896 hsa-asg-11896_st2 12.78 13.36 13.3513.17 0.33 100 11.65 11.91 hsa-asg-11867 hsa-asg-11867_st1 4.90 4.784.65 4.78 0.12 100 2.85 2.89 hsa-asg-11844 hsa-asg-11844_st2 4.08 4.394.12 4.19 0.17 33.33 2.51 2.28 hsa-asg-11764 hsa-asg-11764_st1 5.20 5.165.16 5.17 0.02 100 3.36 3.75 hsa-asg-11572 hsa-asg-11572_st2 12.16 12.5812.04 12.26 0.28 100 10.81 10.76 hsa-asg-11527 hsa-asg-11527_st1 4.384.39 3.87 4.21 0.30 66.66 2.51 2.76 hsa-asg-11515 hsa-asg-11515_st211.93 12.61 12.18 12.24 0.34 100 9.93 10.38 hsa-asg-11486hsa-asg-11486_st1 12.36 12.88 12.34 12.53 0.31 100 10.79 10.88hsa-asg-11443 hsa-asg-11443_st1 8.75 7.37 7.62 7.91 0.74 100 2.99 4.25hsa-asg-11428 hsa-asg-11428_st2 1.19 1.14 2.65 1.66 0.86 0 6.50 5.67hsa-asg-114 hsa-asg-114_st2 9.09 9.12 9.09 9.10 0.02 100 7.40 7.70hsa-asg-11373 hsa-asg-11373_st1 10.53 10.46 10.38 10.46 0.07 100 9.239.31 hsa-asg-11199 hsa-asg-11199_st2 12.41 12.19 12.23 12.28 0.12 1009.96 9.33 hsa-asg-10981 hsa-asg-10981_st1 4.74 4.55 4.88 4.72 0.17 66.663.13 3.34 hsa-asg-10883 hsa-asg-10883_st1 9.08 8.50 8.12 8.57 0.49 1003.98 3.56 hsa-asg-10577 hsa-asg-10577_st1 12.54 12.70 12.75 12.66 0.11100 10.81 10.79 hsa-asg-10473 hsa-asg-10473_st1 5.04 4.33 4.91 4.76 0.3866.66 2.69 2.61 hsa-asg-10409 hsa-asg-10409_st1 4.08 4.39 4.29 4.25 0.1633.33 2.85 2.61 hsa-asg-10401 hsa-asg-10401_st1 4.85 5.22 4.81 4.96 0.23100 3.13 3.13 hsa-asg-10154 hsa-asg-10154_st1 11.77 12.13 11.83 11.910.19 100 8.84 9.78 hsa-asg-10113 hsa-asg-10113_st1 3.80 4.69 4.23 4.240.45 33.33 1.83 1.62 hsa-asg-10065 hsa-asg-10065_st1 3.80 4.49 4.12 4.140.35 33.33 2.31 2.45 hsa-miR257 hsa-miR257_st1 8.77 8.26 9.39 8.81 0.57100 4.12 2.61 hsa-miR229 hsa-miR229_st1 3.45 3.26 2.93 3.21 0.26 0 2.312.28 hsa-miR223 hsa-miR223_st2 8.08 7.69 7.55 7.77 0.28 0 8.71 8.58hsa-miR207 hsa-miR207_st1 9.05 9.13 9.08 9.08 0.04 100 7.26 7.56hsa-miR189 hsa-miR189_st2 8.22 8.77 8.59 8.52 0.28 66.66 7.44 8.09hsa-miR166 hsa-miR166_st1 7.61 7.47 7.72 7.60 0.13 0 7.27 6.88hsa-miR157 hsa-miR157_st2 9.96 8.40 8.87 9.08 0.80 100 5.99 6.22hsa-miR120 hsa-miR120_st2 2.10 1.79 1.65 1.84 0.23 0 2.31 2.28hsa-miR102 hsa-miR102_st2 7.78 8.06 8.39 8.08 0.30 100 6.16 6.22hsa-asg-999 hsa-asg-999_st2 7.27 6.76 8.43 7.49 0.85 100 6.33 6.30hsa-asg-9927 hsa-asg-9927_st2 4.31 3.68 3.64 3.88 0.38 0 6.94 6.79hsa-asg-9886 hsa-asg-9886_st2 8.96 8.76 8.79 8.84 0.11 100 8.25 8.68hsa-asg-9880 hsa-asg-9880_st2 11.60 11.83 11.64 11.69 0.13 100 11.1910.84 hsa-asg-9874 hsa-asg-9874_st1 13.01 13.49 12.92 13.14 0.31 10012.65 12.53 hsa-asg-9858 hsa-asg-9858_st2 8.77 9.18 9.08 9.01 0.21 1006.90 8.29 hsa-asg-9845 hsa-asg-9845_st1 3.32 1.79 1.90 2.33 0.85 0 6.535.47 hsa-asg-9735 hsa-asg-9735_st1 7.00 6.54 6.81 6.78 0.23 100 5.846.60 hsa-asg-9729 hsa-asg-9729_st2 9.18 9.30 9.11 9.20 0.10 100 7.908.11 hsa-asg-9714 hsa-asg-9714_st1 2.10 2.05 1.90 2.02 0.11 0 3.25 3.13hsa-asg-9696 hsa-asg-9696_st1 7.80 5.81 6.32 6.64 1.03 66.66 2.69 4.55hsa-asg-9665 hsa-asg-9665_st2 11.37 11.70 11.78 11.62 0.22 100 12.4312.36 hsa-asg-9649 hsa-asg-9649_st1 3.00 1.79 1.07 1.95 0.98 0 7.32 6.65hsa-asg-9625 hsa-asg-9625_st2 9.96 10.03 10.38 10.13 0.23 100 11.1710.45 hsa-asg-9610 hsa-asg-9610_st1 2.38 2.05 2.12 2.18 0.17 0 4.52 4.69hsa-asg-9575 hsa-asg-9575_st1 6.08 4.98 4.18 5.08 0.96 66.66 3.13 4.19hsa-asg-9573 hsa-asg-9573_st2 6.20 7.96 7.24 7.14 0.88 100 6.13 5.60hsa-asg-9569 hsa-asg-9569_st2 0.97 0.78 1.37 1.04 0.30 0 2.31 1.62hsa-asg-9562 hsa-asg-9562_st2 4.97 4.74 5.56 5.09 0.42 33.33 2.31 3.07hsa-asg-9559 hsa-asg-9559_st2 6.34 5.95 5.74 6.01 0.31 66.66 4.36 3.72hsa-asg-9547 hsa-asg-9547_st2 6.26 6.55 6.13 6.31 0.22 100 6.50 6.73hsa-asg-9530 hsa-asg-9530_st2 13.28 13.61 13.44 13.44 0.16 100 12.6612.64 hsa-asg-9521 hsa-asg-9521_st2 4.24 3.93 2.80 3.65 0.76 33.33 2.082.08 hsa-asg-9508 hsa-asg-9508_st1 12.06 12.47 12.36 12.30 0.21 10010.32 11.21 hsa-asg-9503 hsa-asg-9503_st2 12.20 12.31 12.16 12.22 0.08100 12.62 12.46 hsa-asg-9486 hsa-asg-9486_st2 4.80 4.39 2.93 4.04 0.98 05.02 5.65 hsa-asg-9476 hsa-asg-9476_st1 1.78 3.26 2.49 2.51 0.74 0 4.986.46 hsa-asg-9457 hsa-asg-9457_st1 11.93 11.88 11.86 11.89 0.03 10012.35 12.41 hsa-asg-9434 hsa-asg-9434_st2 9.11 9.14 8.81 9.02 0.18 1008.69 8.70 hsa-asg-9411 hsa-asg-9411_st1 4.90 3.93 4.48 4.44 0.49 66.662.99 3.02 hsa-asg-9368 hsa-asg-9368_st2 8.91 8.48 9.18 8.85 0.36 1007.51 7.41 hsa-asg-9332 hsa-asg-9332_st2 9.83 9.88 9.78 9.83 0.05 1009.67 9.50 hsa-asg-9319 hsa-asg-9319_st2 8.17 8.33 7.87 8.13 0.23 1007.29 7.55 hsa-asg-9297 hsa-asg-9297_st2 6.31 5.51 4.06 5.29 1.14 33.333.57 3.19 hsa-asg-9273 hsa-asg-9273_st2 11.24 11.49 11.31 11.34 0.13 10011.79 11.59 hsa-asg-925 hsa-asg-925_st1 6.71 7.01 6.64 6.79 0.20 1003.25 5.36 hsa-asg-9248 hsa-asg-9248_st2 10.01 10.12 10.03 10.06 0.06 10010.30 10.23 hsa-asg-924 hsa-asg-924_st1 8.38 5.43 6.98 6.93 1.48 1001.83 1.05 hsa-asg-922 hsa-asg-922_st1 7.30 7.80 7.27 7.46 0.30 100 2.995.05 hsa-asg-9212 hsa-asg-9212_st2 2.82 4.17 −0.45 2.18 2.38 0 8.95 9.07hsa-asg-916 hsa-asg-916_st1 10.61 11.19 10.35 10.72 0.43 100 9.84 10.14hsa-asg-914 hsa-asg-914_st1 10.58 10.76 10.35 10.56 0.20 100 10.32 9.74hsa-asg-9128 hsa-asg-9128_st1 8.39 8.42 8.63 8.48 0.13 100 7.78 7.64hsa-asg-9120 hsa-asg-9120_st2 9.59 9.25 8.80 9.22 0.40 100 8.66 8.39hsa-asg-9099 hsa-asg-9099_st2 6.18 7.04 6.04 6.42 0.54 100 4.95 5.55hsa-asg-9092 hsa-asg-9092_st2 12.44 12.49 12.32 12.42 0.08 100 12.9912.57 hsa-asg-9082 hsa-asg-9082_st1 7.28 6.99 8.28 7.52 0.68 33.33 3.316.55 hsa-asg-9060 hsa-asg-9060_st1 8.37 7.94 8.12 8.14 0.21 100 7.237.85 hsa-asg-9052 hsa-asg-9052_st1 7.71 7.51 7.90 7.71 0.20 0 9.06 9.11hsa-asg-9025 hsa-asg-9025_st2 8.23 7.65 7.79 7.89 0.30 100 7.20 7.35hsa-asg-9008 hsa-asg-9008_st2 1.40 2.84 0.42 1.56 1.22 0 5.02 4.11hsa-asg-9004 hsa-asg-9004_st1 9.67 10.40 9.62 9.90 0.44 100 8.69 8.62hsa-asg-8972 hsa-asg-8972_st2 6.35 6.81 6.46 6.54 0.24 100 2.08 4.55hsa-asg-8970 hsa-asg-8970_st2 5.22 4.90 4.95 5.02 0.17 0 6.59 6.68hsa-asg-8957 hsa-asg-8957_st2 9.88 10.19 9.80 9.96 0.21 100 9.50 9.63hsa-asg-8955 hsa-asg-8955_st2 8.59 9.23 7.76 8.53 0.74 100 1.54 6.26hsa-asg-8953 hsa-asg-8953_st1 11.30 11.51 11.19 11.34 0.16 100 10.6010.32 hsa-asg-8928 hsa-asg-8928_st2 8.56 8.61 8.80 8.65 0.13 66.66 8.078.25 hsa-asg-8925 hsa-asg-8925_st2 6.77 1.79 −1.17 2.46 4.02 0 9.22 8.13hsa-asg-8889 hsa-asg-8889_st1 5.44 6.64 6.27 6.11 0.62 66.66 4.24 4.22hsa-asg-8882 hsa-asg-8882_st1 5.95 7.77 7.23 6.98 0.94 100 5.25 6.15hsa-asg-8878 hsa-asg-8878_st2 10.88 11.07 10.92 10.95 0.10 100 10.179.78 hsa-asg-8872 hsa-asg-8872_st1 8.88 8.53 8.71 8.71 0.17 100 10.129.90 hsa-asg-8770 hsa-asg-8770_st1 6.35 5.19 4.98 5.51 0.74 100 4.243.52 hsa-asg-8757 hsa-asg-8757_st2 2.24 1.48 2.12 1.95 0.41 0 5.31 4.17hsa-asg-8699 hsa-asg-8699_st2 3.74 4.21 4.44 4.13 0.35 0 6.04 6.22hsa-asg-8687 hsa-asg-8687_st1 9.48 10.10 9.18 9.59 0.47 100 8.96 8.64hsa-asg-8670 hsa-asg-8670_st2 1.78 1.79 2.12 1.89 0.19 0 3.91 2.76hsa-asg-8642 hsa-asg-8642_st1 6.07 4.82 5.45 5.45 0.63 33.33 4.52 4.11hsa-asg-8623 hsa-asg-8623_st1 10.09 10.07 9.69 9.95 0.23 100 9.73 9.58hsa-asg-8617 hsa-asg-8617_st1 11.23 11.62 10.91 11.25 0.36 100 10.549.86 hsa-asg-8607 hsa-asg-8607_st1 10.34 10.59 10.05 10.33 0.27 100 9.609.71 hsa-asg-8602 hsa-asg-8602_st2 7.54 7.15 7.43 7.37 0.20 0 7.66 7.70hsa-asg-8579 hsa-asg-8579_st2 5.48 4.44 4.23 4.72 0.67 66.66 2.85 2.45hsa-asg-8576 hsa-asg-8576_st2 11.99 12.14 12.11 12.08 0.08 100 11.9511.80 hsa-asg-8520 hsa-asg-8520_st1 10.74 11.10 10.72 10.85 0.22 10010.62 10.48 hsa-asg-8518 hsa-asg-8518_st1 9.15 8.93 9.08 9.06 0.11 1008.22 7.29 hsa-asg-8515 hsa-asg-8515_st2 10.77 10.84 10.60 10.74 0.12 10011.29 11.20 hsa-asg-8510 hsa-asg-8510_st1 10.83 10.51 10.54 10.63 0.18100 9.97 10.28 hsa-asg-8459 hsa-asg-8459_st1 9.01 9.81 9.25 9.36 0.41100 8.78 8.78 hsa-asg-8454 hsa-asg-8454_st1 6.04 5.48 5.82 5.78 0.28 1004.24 3.52 hsa-asg-8450 hsa-asg-8450_st1 10.49 10.75 10.54 10.59 0.14 10011.89 11.69 hsa-asg-8444 hsa-asg-8444_st2 8.08 8.92 8.32 8.44 0.43 1006.31 7.20 hsa-asg-8421 hsa-asg-8421_st1 11.26 11.31 11.48 11.35 0.12 10011.04 10.86 hsa-asg-8411 hsa-asg-8411_st2 6.65 6.74 5.49 6.30 0.70 1004.91 3.52 hsa-asg-8391 hsa-asg-8391_st1 7.73 8.38 8.36 8.16 0.37 1007.21 6.76 hsa-asg-8379 hsa-asg-8379_st2 7.65 6.88 6.80 7.11 0.47 1003.98 5.32 hsa-asg-8357 hsa-asg-8357_st1 4.38 4.33 3.56 4.09 0.46 33.332.69 1.86 hsa-asg-8300 hsa-asg-8300_st2 6.27 6.46 4.06 5.60 1.33 66.664.47 3.64 hsa-asg-8297 hsa-asg-8297_st2 5.08 3.93 4.61 4.54 0.58 66.663.13 3.43 hsa-asg-8282 hsa-asg-8282_st1 3.69 3.58 3.87 3.71 0.14 0 6.066.15 hsa-asg-8280 hsa-asg-8280_st2 0.97 2.29 3.47 2.24 1.25 0 6.97 5.96hsa-asg-8257 hsa-asg-8257_st1 8.07 8.83 8.67 8.53 0.40 100 6.38 7.06hsa-asg-8253 hsa-asg-8253_st2 11.94 12.11 11.78 11.94 0.17 100 11.3211.21 hsa-asg-8227 hsa-asg-8227_st2 9.87 9.93 10.25 10.02 0.20 100 9.599.58 hsa-asg-8221 hsa-asg-8221_st2 10.50 11.37 10.69 10.85 0.46 100 9.839.79 hsa-asg-8197 hsa-asg-8197_st2 6.17 6.32 5.74 6.08 0.30 100 5.315.11 hsa-asg-8163 hsa-asg-8163_st1 12.32 12.24 12.10 12.22 0.11 10012.99 12.77 hsa-asg-8137 hsa-asg-8137_st2 6.67 7.80 7.93 7.47 0.70 1006.13 6.03 hsa-asg-8130 hsa-asg-8130_st2 −0.33 5.60 −1.26 1.34 3.72 08.17 7.23 hsa-asg-8121 hsa-asg-8121_st2 7.96 8.16 8.31 8.14 0.18 33.337.35 7.75 hsa-asg-8099 hsa-asg-8099_st2 2.10 2.05 4.34 2.83 1.31 0 4.715.14 hsa-asg-8084 hsa-asg-8084_st2 1.40 1.48 1.90 1.59 0.26 0 2.08 2.08hsa-asg-8055 hsa-asg-8055_st2 6.50 4.07 4.95 5.17 1.23 66.66 1.83 1.62hsa-asg-8054 hsa-asg-8054_st1 4.24 3.85 3.47 3.85 0.38 33.33 2.08 1.86hsa-asg-8042 hsa-asg-8042_st2 9.27 8.28 9.05 8.87 0.52 100 11.02 11.08hsa-asg-8036 hsa-asg-8036_st2 7.43 6.76 6.61 6.93 0.44 100 4.71 4.59hsa-asg-8002 hsa-asg-8002_st2 9.89 10.42 9.32 9.88 0.55 100 8.43 6.55hsa-asg-7973 hsa-asg-7973_st2 9.76 10.45 9.78 10.00 0.39 100 9.01 8.80hsa-asg-7964 hsa-asg-7964_st2 6.27 5.16 5.49 5.64 0.57 66.66 6.55 7.27hsa-asg-7952 hsa-asg-7952_st2 0.97 1.48 1.65 1.37 0.35 0 2.31 3.68hsa-asg-7939 hsa-asg-7939_st1 13.56 13.72 13.63 13.64 0.08 100 14.1414.01 hsa-asg-7930 hsa-asg-7930_st1 8.06 8.23 8.31 8.20 0.13 66.66 9.698.96 hsa-asg-791 hsa-asg-791_st1 5.08 3.68 4.12 4.29 0.72 33.33 1.832.89 hsa-asg-7900 hsa-asg-7900_st1 10.00 10.66 9.72 10.13 0.48 100 8.008.66 hsa-asg-7859 hsa-asg-7859_st1 12.07 12.23 12.13 12.14 0.08 10011.64 11.47 hsa-asg-7777 hsa-asg-7777_st2 8.62 9.49 9.42 9.18 0.48 66.667.92 7.70 hsa-asg-7770 hsa-asg-7770_st2 8.91 10.10 8.96 9.32 0.68 1007.05 6.94 hsa-asg-7753 hsa-asg-7753_st2 12.46 12.38 12.20 12.35 0.13 10012.90 12.81 hsa-asg-7751 hsa-asg-7751_st2 4.69 6.84 6.79 6.10 1.23 66.663.25 3.64 hsa-asg-7749 hsa-asg-7749_st1 7.60 8.16 7.82 7.86 0.28 33.338.04 8.75 hsa-asg-773 hsa-asg-773_st1 10.55 11.12 11.09 10.92 0.32 1009.19 9.91 hsa-asg-7721 hsa-asg-7721_st1 8.49 8.32 8.93 8.58 0.31 1006.79 7.22 hsa-asg-7704 hsa-asg-7704_st2 8.57 8.69 8.06 8.44 0.34 1007.85 7.75 hsa-asg-7690 hsa-asg-7690_st1 4.63 4.98 4.39 4.67 0.30 1005.39 5.36 hsa-asg-7688 hsa-asg-7688_st1 5.20 4.55 4.91 4.89 0.33 1002.69 2.28 hsa-asg-7684 hsa-asg-7684_st2 2.10 2.05 1.90 2.02 0.11 0 7.454.80 hsa-asg-7667 hsa-asg-7667_st2 11.95 12.15 12.08 12.06 0.10 10011.64 11.61 hsa-asg-7651 hsa-asg-7651_st2 10.12 10.22 10.13 10.16 0.06100 9.67 9.69 hsa-asg-7640 hsa-asg-7640_st2 8.88 8.86 8.88 8.87 0.0166.66 9.28 9.08 hsa-asg-7637 hsa-asg-7637_st2 7.85 6.50 6.88 7.08 0.70100 4.79 2.18 hsa-asg-7617 hsa-asg-7617_st1 8.45 8.84 9.00 8.76 0.2866.66 9.43 9.49 hsa-asg-7585 hsa-asg-7585_st2 5.48 6.15 5.47 5.70 0.39100 7.13 7.03 hsa-asg-7579 hsa-asg-7579_st2 7.91 8.12 7.85 7.96 0.14 1008.34 8.51 hsa-asg-7564 hsa-asg-7564_st1 8.13 6.67 7.67 7.49 0.75 1006.56 6.55 hsa-asg-7540 hsa-asg-7540_st2 6.85 6.01 6.88 6.58 0.50 1008.00 7.40 hsa-asg-7532 hsa-asg-7532_st2 6.57 6.99 6.70 6.76 0.22 1004.95 5.08 hsa-asg-7514 hsa-asg-7514_st1 11.38 11.17 11.22 11.25 0.11 10011.80 11.49 hsa-asg-7510 hsa-asg-7510_st1 11.54 11.08 10.87 11.16 0.34100 10.73 10.69 hsa-asg-7491 hsa-asg-7491_st1 9.11 9.60 9.89 9.53 0.3966.66 9.29 8.58 hsa-asg-7487 hsa-asg-7487_st1 7.04 7.95 8.11 7.70 0.58100 7.29 6.18 hsa-asg-7478 hsa-asg-7478_st1 4.90 4.27 3.87 4.34 0.5233.33 3.25 3.02 hsa-asg-7471 hsa-asg-7471_st1 4.97 6.46 5.56 5.66 0.7566.66 3.57 4.92 hsa-asg-7465 hsa-asg-7465_st2 7.84 5.66 6.38 6.62 1.11100 3.91 2.61 hsa-asg-7463 hsa-asg-7463_st1 8.24 7.96 7.64 7.95 0.3066.66 6.88 7.47 hsa-asg-7458 hsa-asg-7458_st2 1.40 2.29 0.75 1.48 0.77 04.98 5.26 hsa-asg-744 hsa-asg-744_st1 12.32 12.78 12.29 12.46 0.27 10011.93 11.68 hsa-asg-7428 hsa-asg-7428_st1 11.21 11.22 11.32 11.25 0.06100 10.46 10.54 hsa-asg-7426 hsa-asg-7426_st1 9.82 9.66 9.94 9.81 0.14100 8.87 9.32 hsa-asg-7424 hsa-asg-7424_st1 7.41 7.82 6.89 7.37 0.46 07.97 7.84 hsa-asg-7423 hsa-asg-7423_st2 4.27 3.68 6.27 4.74 1.36 33.332.08 2.18 hsa-asg-7410 hsa-asg-7410_st1 8.64 8.40 8.67 8.57 0.15 1008.05 8.06 hsa-asg-7400 hsa-asg-7400_st1 12.21 12.30 11.90 12.14 0.21 10011.21 11.24 hsa-asg-7367 hsa-asg-7367_st2 2.82 2.84 2.12 2.59 0.41 01.83 2.08 hsa-asg-7363 hsa-asg-7363_st1 4.31 3.93 3.87 4.03 0.24 33.332.85 1.62 hsa-asg-7319 hsa-asg-7319_st2 3.80 3.58 3.56 3.65 0.13 0 2.513.13 hsa-asg-7314 hsa-asg-7314_st2 4.63 2.84 2.65 3.38 1.09 0 4.47 6.01hsa-asg-7302 hsa-asg-7302_st2 3.69 3.76 4.39 3.95 0.38 33.33 1.22 2.08hsa-asg-730 hsa-asg-730_st1 9.76 9.98 9.94 9.89 0.12 100 10.62 10.34hsa-asg-7292 hsa-asg-7292_st1 10.18 10.66 10.58 10.47 0.26 100 9.64 9.85hsa-asg-7281 hsa-asg-7281_st1 4.63 3.93 4.12 4.23 0.36 66.66 2.31 2.61hsa-asg-7258 hsa-asg-7258_st2 3.32 2.84 5.76 3.97 1.57 0 6.68 6.92hsa-asg-7252 hsa-asg-7252_st1 4.20 4.27 4.39 4.28 0.10 0 3.98 4.22hsa-asg-7239 hsa-asg-7239_st2 4.74 4.21 4.73 4.56 0.31 66.66 2.99 2.76hsa-asg-723 hsa-asg-723_st2 3.80 3.85 4.23 3.96 0.24 33.33 2.31 2.28hsa-asg-7224 hsa-asg-7224_st2 9.31 9.34 9.25 9.30 0.04 100 8.84 8.63hsa-asg-7221 hsa-asg-7221_st1 10.27 10.66 10.37 10.44 0.20 100 9.65 9.73hsa-asg-7217 hsa-asg-7217_st2 11.48 11.67 11.51 11.56 0.10 100 11.1711.01 hsa-asg-7201 hsa-asg-7201_st2 8.19 8.32 8.44 8.32 0.13 100 7.637.72 hsa-asg-7200 hsa-asg-7200_st2 10.15 10.32 10.36 10.28 0.11 100 9.8510.00 hsa-asg-7199 hsa-asg-7199_st1 10.06 10.70 10.44 10.40 0.32 1009.91 9.57 hsa-asg-7175 hsa-asg-7175_st1 13.26 13.36 13.06 13.23 0.15 10013.82 13.45 hsa-asg-7173 hsa-asg-7173_st1 9.64 9.43 9.95 9.67 0.26 10010.12 10.08 hsa-asg-7097 hsa-asg-7097_st2 11.72 11.80 11.68 11.74 0.06100 11.39 11.37 hsa-asg-7096 hsa-asg-7096_st2 12.57 12.67 12.38 12.540.15 100 13.15 12.78 hsa-asg-7070 hsa-asg-7070_st1 9.38 8.90 9.52 9.270.32 100 9.66 9.78 hsa-asg-7062 hsa-asg-7062_st1 8.14 7.68 8.52 8.110.42 100 8.84 8.81 hsa-asg-7025 hsa-asg-7025_st1 11.26 11.37 11.05 11.230.16 100 10.64 10.14 hsa-asg-7009 hsa-asg-7009_st1 9.46 9.63 9.28 9.460.17 100 8.69 8.35 hsa-asg-6926 hsa-asg-6926_st2 5.90 5.05 4.65 5.200.64 66.66 3.75 3.83 hsa-asg-6922 hsa-asg-6922_st2 2.38 3.76 2.93 3.020.70 0 1.54 1.35 hsa-asg-6910 hsa-asg-6910_st2 9.93 10.06 10.21 10.070.14 100 7.44 8.78 hsa-asg-6898 hsa-asg-6898_st2 4.74 3.68 4.18 4.200.53 33.33 3.13 2.45 hsa-asg-6883 hsa-asg-6883_st1 5.38 5.09 3.56 4.680.98 66.66 2.08 2.45 hsa-asg-6860 hsa-asg-6860_st1 10.79 10.53 10.7210.68 0.14 100 9.87 9.66 hsa-asg-6856 hsa-asg-6856_st2 4.85 4.55 4.534.64 0.18 33.33 4.30 3.83 hsa-asg-6831 hsa-asg-6831_st2 9.60 9.67 9.689.65 0.05 100 10.45 10.19 hsa-asg-6823 hsa-asg-6823_st1 8.06 8.04 8.608.23 0.32 100 6.83 7.02 hsa-asg-682 hsa-asg-682_st2 7.97 7.14 7.65 7.590.42 100 6.75 6.96 hsa-asg-6782 hsa-asg-6782_st1 6.98 7.08 7.20 7.090.11 100 6.18 6.95 hsa-asg-6754 hsa-asg-6754_st1 5.35 4.27 4.00 4.540.71 0 6.28 6.79 hsa-asg-6733 hsa-asg-6733_st2 4.85 4.94 4.77 4.85 0.09100 4.24 2.76 hsa-asg-6729 hsa-asg-6729_st2 9.04 9.98 9.09 9.37 0.53 1007.92 8.13 hsa-asg-6728 hsa-asg-6728_st2 9.83 10.07 10.10 10.00 0.15 10011.24 10.59 hsa-asg-6633 hsa-asg-6633_st2 6.01 6.31 5.04 5.78 0.66 66.664.30 4.55 hsa-asg-657 hsa-asg-657_st1 8.99 8.56 9.59 9.05 0.52 66.667.40 8.08 hsa-asg-6569 hsa-asg-6569_st2 7.23 7.23 7.86 7.44 0.36 1005.86 6.35 hsa-asg-6532 hsa-asg-6532_st1 4.38 4.90 3.94 4.41 0.48 66.663.13 3.02 hsa-asg- hsa-asg-6507_st2 11.52 11.48 11.35 11.45 0.09 10012.50 12.26 6507_st2 hsa-asg-6500 hsa-asg-6500_st1 8.77 8.25 8.77 8.600.30 100 8.14 7.90 hsa-asg-6466 hsa-asg-6466_st1 11.71 11.74 11.69 11.710.03 100 12.19 11.79 hsa-asg-644 hsa-asg-644_st1 7.94 8.26 6.70 7.630.83 100 4.71 6.35 hsa-asg-6412 hsa-asg-6412_st2 8.89 9.24 9.65 9.260.38 66.66 7.71 8.92 hsa-asg-641 hsa-asg-641_st2 3.69 2.99 4.12 3.600.57 0 1.54 2.28 hsa-asg-6383 hsa-asg-6383_st1 8.83 9.49 9.55 9.29 0.4066.66 8.24 8.41 hsa-asg-6371 hsa-asg-6371_st2 11.07 11.44 11.31 11.270.19 100 10.94 10.82 hsa-asg-6341 hsa-asg-6341_st2 12.94 12.85 12.5812.79 0.18 100 13.74 13.55 hsa-asg-6331 hsa-asg-6331_st2 8.47 8.70 8.758.64 0.15 100 8.15 8.30 hsa-asg-6239 hsa-asg-6239_st2 2.82 3.37 2.492.90 0.45 0 2.08 1.05 hsa-asg-6203 hsa-asg-6203_st2 10.83 10.99 11.1410.99 0.16 100 11.92 11.58 hsa-asg-6202 hsa-asg-6202_st2 8.80 8.64 8.448.63 0.18 100 7.99 7.85 hsa-asg-6186 hsa-asg-6186_st2 5.89 5.19 7.016.03 0.92 66.66 4.24 3.93 hsa-asg-6180 hsa-asg-6180_st1 11.75 11.8211.74 11.77 0.05 100 11.38 10.91 hsa-asg-6168 hsa-asg-6168_st1 1.78 2.051.90 1.91 0.14 0 2.69 2.76 hsa-asg-6165 hsa-asg-6165_st2 1.40 2.99 3.062.49 0.94 0 4.57 4.35 hsa-asg-6149 hsa-asg-6149_st2 10.50 10.69 10.5210.57 0.11 100 9.82 9.66 hsa-asg-6138 hsa-asg-6138_st1 7.09 8.06 8.177.77 0.60 100 3.91 5.02 hsa-asg-6116 hsa-asg-6116_st2 1.78 1.79 1.901.82 0.07 0 2.85 3.34 hsa-asg-6092 hsa-asg-6092_st2 11.53 11.37 11.4911.46 0.09 66.66 12.21 12.04 hsa-asg-6071 hsa-asg-6071_st2 4.99 5.405.84 5.41 0.42 0 6.69 6.80 hsa-asg-6021 hsa-asg-6021_st2 9.84 9.55 9.839.74 0.16 100 9.46 9.50 hsa-asg-6019 hsa-asg-6019_st1 6.63 7.21 6.206.68 0.51 0 8.56 7.98 hsa-asg-6018 hsa-asg-6018_st2 9.25 9.15 10.01 9.470.47 100 10.35 10.32 hsa-asg-6000 hsa-asg-6000_st1 8.39 8.49 8.11 8.330.19 33.33 7.83 6.81 hsa-asg-5960 hsa-asg-5960_st2 11.11 11.54 11.0511.23 0.26 100 10.35 10.26 hsa-asg-5952 hsa-asg-5952_st2 5.97 6.36 5.405.91 0.49 66.66 4.18 4.17 hsa-asg-5889 hsa-asg-5889_st2 7.62 7.59 7.807.67 0.11 100 4.05 5.71 hsa-asg-5873 hsa-asg-5873_st2 5.55 5.02 4.535.03 0.51 100 2.51 3.83 hsa-asg-585 hsa-asg-585_st2 12.45 12.36 12.1412.32 0.16 100 12.81 12.56 hsa-asg-5827 hsa-asg-5827_st2 7.85 7.43 7.127.47 0.37 100 3.57 4.59 hsa-asg-5810 hsa-asg-5810_st2 9.99 9.79 9.499.76 0.25 100 10.08 10.10 hsa-asg-5773 hsa-asg-5773_st2 7.70 8.93 8.218.28 0.62 33.33 6.91 6.65 hsa-asg-5728 hsa-asg-5728_st2 10.73 11.3211.13 11.06 0.30 100 9.14 9.99 hsa-asg-572 hsa-asg-572_st2 8.06 9.678.85 8.86 0.81 66.66 7.61 7.14 hsa-asg-5695 hsa-asg-5695_st2 3.90 4.144.84 4.29 0.49 33.33 2.69 2.76 hsa-asg-562 hsa-asg-562_st1 10.65 9.069.79 9.84 0.80 100 8.07 6.86 hsa-asg-5519 hsa-asg-5519_st1 4.63 5.075.85 5.19 0.62 0 6.79 7.19 hsa-asg-5511 hsa-asg-5511_st2 7.82 8.13 9.188.38 0.71 100 7.05 7.77 hsa-asg-547 hsa-asg-547_st1 2.61 2.49 2.49 2.530.07 0 1.54 2.08 hsa-asg-5467 hsa-asg-5467_st1 3.00 2.84 3.06 2.97 0.110 4.24 3.96 hsa-asg-5451 hsa-asg-5451_st2 5.75 5.16 5.40 5.43 0.30 1002.69 3.68 hsa-asg-5421 hsa-asg-5421_st1 8.23 8.49 8.11 8.28 0.19 1007.08 7.59 hsa-asg-5399 hsa-asg-5399_st1 7.31 8.69 9.30 8.43 1.02 66.6610.21 10.46 hsa-asg-5365 hsa-asg-5365_st2 4.08 5.51 3.72 4.43 0.95 05.52 6.37 hsa-asg-5326 hsa-asg-5326_st2 6.93 6.16 5.68 6.26 0.63 66.668.20 7.96 hsa-asg-5304 hsa-asg-5304_st1 9.42 8.41 8.91 8.91 0.51 33.337.21 7.06 hsa-asg-5290 hsa-asg-5290_st2 3.00 3.48 2.65 3.05 0.42 0 5.314.77 hsa-asg-529 hsa-asg-529_st1 1.40 2.29 2.12 1.94 0.47 0 4.83 3.68hsa-asg-5289 hsa-asg-5289_st2 8.95 9.13 9.22 9.10 0.14 66.66 9.32 9.79hsa-asg-5240 hsa-asg-5240_st1 4.16 4.21 4.00 4.12 0.11 100 3.36 3.60hsa-asg-5227 hsa-asg-5227_st1 5.01 6.16 5.68 5.62 0.58 66.66 5.16 4.22hsa-asg-5176 hsa-asg-5176_st2 5.37 5.09 5.97 5.48 0.45 33.33 2.69 3.64hsa-asg-5134 hsa-asg-5134_st2 3.32 4.69 3.38 3.79 0.78 33.33 2.85 2.08hsa-asg-5122 hsa-asg-5122_st2 10.19 11.33 10.77 10.76 0.57 100 9.39 9.12hsa-asg-5115 hsa-asg-5115_st1 10.96 10.97 10.98 10.97 0.01 100 9.5310.17 hsa-asg-5109 hsa-asg-5109_st1 11.62 12.01 11.75 11.79 0.20 10011.13 11.36 hsa-asg-509 hsa-asg-509_st1 2.38 2.49 2.12 2.33 0.19 0 4.873.68 hsa-asg-5085 hsa-asg-5085_st2 10.83 11.14 10.68 10.88 0.24 10010.56 10.39 hsa-asg-5061 hsa-asg-5061_st1 8.76 8.76 9.43 8.98 0.39 10010.07 9.53 hsa-asg-5053 hsa-asg-5053_st1 4.85 4.44 4.57 4.62 0.21 66.664.05 3.13 hsa-asg-5005 hsa-asg-5005_st2 9.30 9.55 9.35 9.40 0.13 1008.69 7.96 hsa-asg-5000 hsa-asg-5000_st2 6.96 6.88 6.86 6.90 0.06 1005.06 6.65 hsa-asg-4990 hsa-asg-4990_st2 10.28 10.69 10.17 10.38 0.27 1008.86 9.24 hsa-asg-4971 hsa-asg-4971_st1 9.99 10.19 9.78 9.99 0.21 1009.58 9.34 hsa-asg-4914 hsa-asg-4914_st1 4.08 2.68 4.98 3.91 1.16 33.331.22 1.05 hsa-asg-4912 hsa-asg-4912_st2 11.30 11.21 11.31 11.27 0.05 10010.43 10.45 hsa-asg-4892 hsa-asg-4892_st2 3.99 4.14 3.87 4.00 0.14 03.75 3.52 hsa-asg-4854 hsa-asg-4854_st2 8.29 8.18 7.83 8.10 0.24 1009.21 9.07 hsa-asg-4847 hsa-asg-4847_st1 9.84 10.08 9.90 9.94 0.12 1009.36 9.37 hsa-asg-4844 hsa-asg-4844_st2 8.88 9.58 9.52 9.33 0.39 1009.97 9.65 hsa-asg-4840 hsa-asg-4840_st1 6.78 5.40 5.58 5.92 0.75 1003.75 3.24 hsa-asg-4839 hsa-asg-4839_st2 12.31 12.57 12.35 12.41 0.14 10012.91 12.63 hsa-asg-481 hsa-asg-481_st1 5.28 3.76 3.06 4.03 1.13 33.331.83 1.86 hsa-asg-4722 hsa-asg-4722_st1 6.48 6.46 6.96 6.64 0.29 0 7.717.92 hsa-asg-471 hsa-asg-471_st1 10.07 10.26 10.11 10.15 0.10 100 10.7710.72 hsa-asg-4703 hsa-asg-4703_st2 5.64 6.90 6.73 6.42 0.68 100 8.167.55 hsa-asg-47 hsa-asg-47_st1 11.40 11.40 10.93 11.24 0.27 100 10.4610.71 hsa-asg-468 hsa-asg-468_st1 9.55 9.98 9.40 9.64 0.30 100 8.10 8.52hsa-asg-4654 hsa-asg-4654_st1 11.98 12.08 12.01 12.02 0.05 100 12.3312.19 hsa-asg-4618 hsa-asg-4618_st1 6.20 5.77 5.40 5.79 0.40 66.66 7.237.11 hsa-asg-459 hsa-asg-459_st1 11.70 11.76 11.86 11.77 0.08 100 12.4512.28 hsa-asg-4580 hsa-asg-4580_st2 5.20 5.05 5.42 5.23 0.19 0 6.30 6.57hsa-asg-458 hsa-asg-458_st1 7.46 7.31 7.86 7.54 0.28 0 9.51 10.08hsa-asg-4529 hsa-asg-4529_st1 10.96 11.46 10.78 11.07 0.36 100 10.299.81 hsa-asg-4469 hsa-asg-4469_st1 9.65 9.87 9.73 9.75 0.11 100 8.248.65 hsa-asg-4458 hsa-asg-4458_st1 9.13 9.19 8.58 8.97 0.34 100 7.087.93 hsa-asg-4448 hsa-asg-4448_st1 4.16 4.14 4.61 4.30 0.27 33.33 2.083.02 hsa-asg-430 hsa-asg-430_st1 2.10 1.48 1.65 1.74 0.32 0 5.06 4.49hsa-asg-4262 hsa-asg-4262_st1 8.80 8.32 8.58 8.56 0.24 100 7.55 8.37hsa-asg-425 hsa-asg-425_st2 10.24 10.38 9.28 9.97 0.60 100 8.16 7.94hsa-asg-4236 hsa-asg-4236_st1 4.80 4.07 4.91 4.59 0.46 66.66 3.25 3.52hsa-asg-4223 hsa-asg-4223_st2 12.63 12.46 12.66 12.58 0.10 100 11.8811.86 hsa-asg-4222 hsa-asg-4222_st2 9.28 9.84 9.14 9.42 0.37 100 6.978.03 hsa-asg-4209 hsa-asg-4209_st2 10.21 10.68 10.42 10.44 0.24 100 9.619.38 hsa-asg-4199 hsa-asg-4199_st1 7.62 7.71 8.10 7.81 0.26 100 7.427.29 hsa-asg-4172 hsa-asg-4172_st2 5.12 4.39 4.91 4.81 0.38 100 3.133.13 hsa-asg-4146 hsa-asg-4146_st2 9.38 9.89 9.12 9.46 0.39 100 8.808.73 hsa-asg-4122 hsa-asg-4122_st2 5.04 3.68 4.65 4.46 0.70 66.66 2.513.52 hsa-asg-412 hsa-asg-412_st1 9.74 9.95 9.69 9.79 0.14 100 9.66 9.43hsa-asg-4114 hsa-asg-4114_st2 5.67 5.31 4.44 5.14 0.63 100 3.91 3.60hsa-asg-4075 hsa-asg-4075_st1 4.69 2.68 3.47 3.61 1.01 33.33 2.08 2.45hsa-asg-4064 hsa-asg-4064_st1 1.40 1.79 3.28 2.16 0.99 0 4.36 5.91hsa-asg-4061 hsa-asg-4061_st1 10.38 10.70 10.12 10.40 0.29 100 9.47 9.55hsa-asg-4059 hsa-asg-4059_st1 9.52 9.75 9.52 9.60 0.14 100 10.31 9.86hsa-asg-4057 hsa-asg-4057_st1 2.38 2.05 2.49 2.31 0.23 0 1.83 1.86hsa-asg-4045 hsa-asg-4045_st1 3.39 4.00 3.80 3.73 0.31 0 5.93 5.02hsa-asg-4023 hsa-asg-4023_st2 8.67 8.59 9.30 8.86 0.39 66.66 9.63 9.64hsa-asg-4019 hsa-asg-4019_st1 7.34 7.47 7.37 7.39 0.07 100 6.98 7.12hsa-asg-4005 hsa-asg-4005_st2 6.65 6.01 7.05 6.57 0.53 100 7.50 8.02hsa-asg-4002 hsa-asg-4002_st1 9.44 8.91 8.61 8.99 0.42 100 7.79 7.96hsa-asg-3999 hsa-asg-3999_st1 11.25 11.46 11.09 11.27 0.18 100 10.7410.86 hsa-asg-3988 hsa-asg-3988_st2 9.18 9.04 9.12 9.11 0.07 100 8.758.80 hsa-asg-3983 hsa-asg-3983_st1 11.29 12.10 11.41 11.60 0.44 10010.34 10.13 hsa-asg-3977 hsa-asg-3977_st2 6.37 6.13 6.37 6.29 0.14 1003.66 5.03 hsa-asg-3970 hsa-asg-3970_st2 6.95 5.66 6.59 6.40 0.67 1004.36 4.57 hsa-asg-395 hsa-asg-395_st2 9.38 9.26 9.19 9.28 0.09 100 9.9010.05 hsa-asg-3941 hsa-asg-3941_st2 10.20 10.91 10.15 10.42 0.43 1009.34 9.22 hsa-asg-3915 hsa-asg-3915_st1 10.89 10.76 10.75 10.80 0.08 10010.57 10.55 hsa-asg-3910 hsa-asg-3910_st2 4.90 4.98 6.53 5.47 0.92 33.331.22 1.97 hsa-asg-3882 hsa-asg-3882_st1 6.16 6.24 6.23 6.21 0.04 1004.91 5.93 hsa-asg-3854 hsa-asg-3854_st1 4.99 4.60 5.04 4.88 0.24 1002.51 1.05 hsa-asg-3842 hsa-asg-3842_st1 4.85 4.33 2.80 3.99 1.06 33.332.69 2.28 hsa-asg-3822 hsa-asg-3822_st2 4.31 4.33 6.29 4.98 1.14 33.332.69 3.47 hsa-asg-3807 hsa-asg-3807_st1 4.69 3.68 6.23 4.86 1.28 66.662.85 2.76 hsa-asg-3786 hsa-asg-3786_st2 8.52 8.27 8.38 8.39 0.13 1008.50 9.00 hsa-asg-3777 hsa-asg-3777_st2 8.05 7.97 8.39 8.14 0.22 33.339.34 8.77 hsa-asg-377 hsa-asg-377_st2 11.96 12.35 11.94 12.08 0.23 10011.65 11.55 hsa-asg-3722 hsa-asg-3722_st1 10.92 11.60 11.04 11.19 0.36100 9.77 10.24 hsa-asg-3715 hsa-asg-3715_st1 9.64 9.32 9.67 9.54 0.1933.33 10.24 10.50 hsa-asg-3714 hsa-asg-3714_st1 7.27 7.44 6.91 7.21 0.27100 7.97 8.02 hsa-asg-3694 hsa-asg-3694_st1 7.31 7.88 8.16 7.78 0.43 08.76 8.50 hsa-asg-3654 hsa-asg-3654_st1 8.60 8.82 8.66 8.69 0.11 66.666.42 7.42 hsa-asg-3637 hsa-asg-3637_st1 11.38 11.37 11.63 11.46 0.15 10011.08 10.72 hsa-asg-3551 hsa-asg-3551_st1 4.69 4.27 4.77 4.58 0.27 66.662.51 3.96 hsa-asg-355 hsa-asg-355_st1 4.31 4.00 4.18 4.16 0.15 33.333.13 2.89 hsa-asg-3516 hsa-asg-3516_st1 3.80 3.13 3.80 3.57 0.38 0 4.714.35 hsa-asg-3513 hsa-asg-3513_st1 4.38 4.14 4.44 4.32 0.16 66.66 3.573.02 hsa-asg-3464 hsa-asg-3464_st2 13.22 13.23 13.24 13.23 0.01 10013.88 13.71 hsa-asg-3462 hsa-asg-3462_st2 11.68 11.58 11.53 11.59 0.08100 12.42 12.22 hsa-asg-3383 hsa-asg-3383_st1 12.14 12.43 12.45 12.340.18 100 11.74 11.84 hsa-asg-3360 hsa-asg-3360_st2 8.17 8.02 7.95 8.050.12 100 7.57 7.44 hsa-asg-3345 hsa-asg-3345_st1 11.91 12.34 12.05 12.100.22 100 11.70 11.43 hsa-asg-3296 hsa-asg-3296_st1 5.10 5.51 5.84 5.480.37 33.33 3.98 4.92 hsa-asg-3291 hsa-asg-3291_st2 7.68 7.60 7.91 7.730.16 0 8.82 8.30 hsa-asg-3290 hsa-asg-3290_st1 9.71 9.57 9.71 9.66 0.08100 9.36 9.13 hsa-asg-3278 hsa-asg-3278_st1 10.38 10.02 10.44 10.28 0.22100 8.33 8.38 hsa-asg-3271 hsa-asg-3271_st1 10.19 10.33 10.30 10.27 0.07100 11.26 10.97 hsa-asg-3265 hsa-asg-3265_st1 7.10 7.75 7.99 7.61 0.46100 3.98 6.41 hsa-asg-3231 hsa-asg-3231_st2 12.91 13.28 12.83 13.00 0.24100 12.28 12.30 hsa-asg-32 hsa-asg-32_st2 7.05 7.22 7.05 7.10 0.10 1007.59 8.07 hsa-asg-3199 hsa-asg-3199_st1 6.49 7.07 7.39 6.98 0.46 1005.67 5.55 hsa-asg-3186 hsa-asg-3186_st2 11.88 11.72 11.81 11.80 0.08 10011.58 11.62 hsa-asg-3182 hsa-asg-3182_st1 9.28 9.76 9.80 9.61 0.29 1008.46 7.90 hsa-asg-3156 hsa-asg-3156_st1 8.17 9.19 7.76 8.37 0.73 1006.59 6.93 hsa-asg-3130 hsa-asg-3130_st1 11.67 11.42 11.63 11.57 0.13 10012.13 11.73 hsa-asg-3124 hsa-asg-3124_st2 4.74 5.58 4.34 4.89 0.63 66.661.22 2.28 hsa-asg-3112 hsa-asg-3112_st1 8.75 8.35 8.51 8.54 0.20 1009.58 9.35 hsa-asg-3101 hsa-asg-3101_st2 9.49 9.86 9.08 9.48 0.39 1008.65 8.70 hsa-asg-3073 hsa-asg-3073_st2 8.71 7.99 7.84 8.18 0.46 1009.37 9.44 hsa-asg-3070 hsa-asg-3070_st2 4.87 5.37 4.91 5.05 0.28 0 2.082.96 hsa-asg-3046 hsa-asg-3046_st2 10.48 9.72 10.11 10.10 0.38 100 9.429.36 hsa-asg-3038 hsa-asg-3038_st2 5.28 5.56 5.30 5.38 0.16 0 5.12 4.95hsa-asg-3015 hsa-asg-3015_st1 1.78 1.48 2.93 2.07 0.77 0 6.74 3.24hsa-asg-3013 hsa-asg-3013_st1 6.70 6.47 6.71 6.62 0.14 0 7.08 7.21hsa-asg-2981 hsa-asg-2981_st2 5.12 5.09 4.98 5.06 0.07 100 4.71 4.49hsa-asg-2966 hsa-asg-2966_st2 8.93 8.32 9.29 8.85 0.49 0 7.90 7.92hsa-asg-2952 hsa-asg-2952_st1 3.69 3.68 4.44 3.93 0.43 33.33 3.13 2.45hsa-asg-2940 hsa-asg-2940_st1 4.97 4.98 4.53 4.82 0.26 0 3.91 3.79hsa-asg-2930 hsa-asg-2930_st1 4.74 4.86 4.12 4.58 0.40 33.33 2.51 2.08hsa-asg-2924 hsa-asg-2924_st1 6.46 5.75 6.33 6.18 0.38 100 1.83 2.76hsa-asg-2899 hsa-asg-2899_st2 12.75 12.82 12.51 12.69 0.16 100 13.1512.95 hsa-asg-2872 hsa-asg-2872_st2 12.28 11.98 12.43 12.23 0.23 10011.86 11.59 hsa-asg-2862 hsa-asg-2862_st1 10.12 10.53 10.25 10.30 0.21100 9.72 9.71 hsa-asg-2858 hsa-asg-2858_st1 9.64 9.87 10.11 9.87 0.24100 9.68 9.56 hsa-asg-2856 hsa-asg-2856_st1 12.06 12.17 11.82 12.01 0.18100 10.38 10.39 hsa-asg-2855 hsa-asg-2855_st2 4.08 3.37 3.87 3.77 0.36 02.51 2.61 hsa-asg-2843 hsa-asg-2843_st1 3.69 3.13 3.47 3.43 0.28 0 2.852.28 hsa-asg-2660 hsa-asg-2660_st1 6.29 3.76 5.54 5.20 1.30 66.66 10.009.02 hsa-asg-265 hsa-asg-265_st2 9.13 8.98 9.04 9.05 0.08 100 9.55 9.20hsa-asg-2620 hsa-asg-2620_st1 5.12 5.34 4.95 5.14 0.20 100 2.85 3.34hsa-asg-2588 hsa-asg-2588_st1 10.17 10.20 10.57 10.31 0.22 100 9.94 9.72hsa-asg-2580 hsa-asg-2580_st1 11.90 12.23 12.21 12.11 0.18 100 11.6511.38 hsa-asg-257 hsa-asg-257_st2 3.45 4.55 4.18 4.06 0.56 66.66 3.252.89 hsa-asg-2565 hsa-asg-2565_st2 5.83 6.39 4.65 5.62 0.89 100 2.992.45 hsa-asg-2563 hsa-asg-2563_st2 9.34 9.70 9.56 9.53 0.18 100 8.808.96 hsa-asg-2542 hsa-asg-2542_st1 7.06 6.01 6.62 6.56 0.53 0 9.62 8.31hsa-asg-2541 hsa-asg-2541_st1 8.80 9.61 9.27 9.23 0.41 66.66 8.61 8.91hsa-asg-2473 hsa-asg-2473_st1 6.06 5.99 5.62 5.89 0.23 100 7.67 7.37hsa-asg-247 hsa-asg-247_st1 8.88 9.05 8.98 8.97 0.08 100 8.44 8.47hsa-asg-2459 hsa-asg-2459_st1 3.99 2.99 5.13 4.04 1.07 33.33 2.08 2.89hsa-asg-2449 hsa-asg-2449_st1 2.10 2.68 2.93 2.57 0.43 0 7.65 4.55hsa-asg-2437 hsa-asg-2437_st1 8.43 8.48 8.37 8.43 0.06 100 8.20 8.23hsa-asg-2433 hsa-asg-2433_st2 3.32 2.99 3.80 3.37 0.40 0 4.12 4.49hsa-asg-2401 hsa-asg-2401_st2 9.94 9.85 9.43 9.74 0.27 100 9.50 9.28hsa-asg-2371 hsa-asg-2371_st1 3.17 2.68 3.17 3.01 0.28 0 2.31 1.35hsa-asg-2365 hsa-asg-2365_st1 4.80 5.19 6.00 5.33 0.62 33.33 2.85 1.48hsa-asg-2273 hsa-asg-2273_st1 4.69 4.82 4.98 4.83 0.15 100 2.31 2.45hsa-asg-2267 hsa-asg-2267_st2 8.33 8.74 8.48 8.52 0.20 100 8.78 9.54hsa-asg-2237 hsa-asg-2237_st1 5.24 5.56 4.12 4.97 0.75 0 6.64 6.30hsa-asg-2228 hsa-asg-2228_st1 10.84 11.20 10.79 10.95 0.22 100 9.85 9.98hsa-asg-2199 hsa-asg-2199_st1 11.56 11.55 11.56 11.56 0.01 100 12.1711.84 hsa-asg-2195 hsa-asg-2195_st1 11.69 11.76 11.62 11.69 0.07 10012.41 11.96 hsa-asg-2174 hsa-asg-2174_st1 8.45 9.11 9.06 8.87 0.37 08.05 7.86 hsa-asg-2160 hsa-asg-2160_st1 7.79 8.03 7.75 7.86 0.16 1008.36 7.98 hsa-asg-2149 hsa-asg-2149_st1 7.20 7.18 7.10 7.16 0.05 1007.56 7.37 hsa-asg-2138 hsa-asg-2138_st2 9.84 9.13 9.34 9.44 0.37 33.339.09 8.68 hsa-asg-2094 hsa-asg-2094_st1 5.67 4.90 5.01 5.19 0.41 1003.47 2.08 hsa-asg-1981 hsa-asg-1981_st1 7.19 8.94 7.17 7.77 1.02 1005.55 5.71 hsa-asg-1978 hsa-asg-1978_st2 4.51 4.27 4.53 4.44 0.14 66.663.91 3.02 hsa-asg-1941 hsa-asg-1941_st2 3.00 2.49 3.06 2.85 0.31 0 3.363.52 hsa-asg-1911 hsa-asg-1911_st1 8.84 9.09 9.29 9.08 0.23 66.66 10.109.54 hsa-asg-1888 hsa-asg-1888_st1 10.59 10.94 10.91 10.81 0.20 10010.39 9.90 hsa-asg-1861 hsa-asg-1861_st1 9.86 10.25 10.01 10.04 0.20 10010.68 10.47 hsa-asg-1858 hsa-asg-1858_st1 12.77 13.03 12.51 12.77 0.26100 11.81 11.88 hsa-asg-1852 hsa-asg-1852_st1 3.63 4.39 4.23 4.08 0.40 04.75 4.92 hsa-asg-1838 hsa-asg-1838_st2 6.69 5.37 6.94 6.33 0.84 66.665.02 5.19 hsa-asg-1827 hsa-asg-1827_st2 1.78 1.79 1.65 1.74 0.08 0 3.753.24 hsa-asg-1813 hsa-asg-1813_st2 10.95 10.93 11.27 11.05 0.19 10011.63 11.51 hsa-asg-1809 hsa-asg-1809_st1 10.89 11.00 11.07 10.99 0.09100 10.90 10.67 hsa-asg-18 hsa-asg-18_st1 7.73 7.24 7.83 7.60 0.31 08.58 8.14 hsa-asg-1797 hsa-asg-1797_st2 9.06 9.47 9.35 9.29 0.21 1009.92 9.76 hsa-asg-1775 hsa-asg-1775_st2 9.11 9.36 9.51 9.33 0.20 10010.28 9.83 hsa-asg-1766 hsa-asg-1766_st2 4.45 4.14 4.00 4.20 0.23 33.333.66 1.86 hsa-asg-1755 hsa-asg-1755_st2 4.45 5.61 4.34 4.80 0.70 66.662.69 1.62 hsa-asg-1747 hsa-asg-1747_st1 6.52 6.67 6.88 6.69 0.18 1004.91 4.89 hsa-asg-1736 hsa-asg-1736_st1 10.68 10.97 10.97 10.87 0.17 10010.13 9.33 hsa-asg-1727 hsa-asg-1727_st2 8.64 9.00 8.62 8.76 0.21 66.668.11 8.08 hsa-asg-1726 hsa-asg-1726_st1 10.34 10.62 10.28 10.41 0.19 10011.09 10.94 hsa-asg-1716 hsa-asg-1716_st1 8.07 8.09 8.34 8.17 0.15 33.339.23 9.05 hsa-asg-1699 hsa-asg-1699_st2 10.35 10.55 10.73 10.54 0.19 1009.92 9.80 hsa-asg-1686 hsa-asg-1686_st1 8.17 8.42 8.35 8.32 0.13 66.667.63 8.00 hsa-asg-1641 hsa-asg-1641_st1 9.17 10.23 9.95 9.78 0.55 1008.99 8.87 hsa-asg-1591 hsa-asg-1591_st2 7.78 7.43 7.61 7.61 0.17 1008.70 8.88 hsa-asg-1588 hsa-asg-1588_st1 6.63 6.15 6.20 6.32 0.26 1004.30 5.92 hsa-asg-1573 hsa-asg-1573_st2 11.01 11.06 11.05 11.04 0.03 10011.36 11.27 hsa-asg-156 hsa-asg-156_st2 7.27 7.08 6.51 6.96 0.40 1006.22 6.62 hsa-asg-1544 hsa-asg-1544_st1 4.74 4.94 4.53 4.74 0.21 1002.69 1.86 hsa-asg-1534 hsa-asg-1534_st1 3.32 3.37 4.39 3.69 0.60 33.331.83 1.86 hsa-asg-1532 hsa-asg-1532_st1 6.49 5.69 6.78 6.32 0.56 0 8.627.73 hsa-asg-1519 hsa-asg-1519_st1 6.26 6.72 6.66 6.55 0.25 100 5.691.86 hsa-asg-1482 hsa-asg-1482_st1 4.94 6.04 4.34 5.11 0.86 100 3.913.89 hsa-asg-145 hsa-asg-145_st2 10.76 11.62 10.99 11.12 0.44 100 10.1910.04 hsa-asg-1448 hsa-asg-1448_st1 9.54 9.88 9.14 9.52 0.37 66.66 8.177.58 hsa-asg-14242 hsa-asg-14242_st2 5.30 5.79 4.34 5.14 0.74 0 7.576.32 hsa-asg-14237 hsa-asg-14237_st2 9.94 9.45 9.62 9.67 0.25 100 9.208.77 hsa-asg-14233 hsa-asg-14233_st1 9.21 9.66 9.82 9.56 0.32 100 7.627.03 hsa-asg-14217 hsa-asg-14217_st1 9.61 9.29 9.04 9.31 0.28 100 6.516.53 hsa-asg-1420 hsa-asg-1420_st2 10.17 10.61 10.62 10.47 0.26 100 9.188.97 hsa-asg-14198 hsa-asg-14198_st2 3.90 3.48 4.91 4.10 0.74 66.66 2.081.86 hsa-asg-14197 hsa-asg-14197_st1 13.05 13.07 12.86 12.99 0.12 10013.46 13.23 hsa-asg-14189 hsa-asg-14189_st2 15.21 15.06 14.80 15.02 0.21100 14.60 14.43 hsa-asg-14176 hsa-asg-14176_st1 8.31 8.40 8.30 8.34 0.06100 8.18 6.50 hsa-asg-14136 hsa-asg-14136_st2 8.47 8.35 8.38 8.40 0.07100 8.66 8.52 hsa-asg-14135 hsa-asg-14135_st1 5.52 3.85 5.37 4.91 0.9266.66 3.36 3.83 hsa-asg-14133 hsa-asg-14133_st2 6.85 6.87 7.42 7.05 0.32100 4.61 6.17 hsa-asg-14068 hsa-asg-14068_st2 13.22 13.21 13.08 13.170.08 100 13.69 13.41 hsa-asg-14066 hsa-asg-14066_st2 8.66 8.66 8.77 8.690.07 66.66 9.41 9.16 hsa-asg-14053 hsa-asg-14053_st2 4.94 5.27 3.38 4.531.01 0 6.12 6.06 hsa-asg-14034 hsa-asg-14034_st1 13.49 13.52 13.30 13.430.12 100 13.13 13.01 hsa-asg-14026 hsa-asg-14026_st1 2.38 1.48 2.31 2.060.50 0 2.99 3.13 hsa-asg-14002 hsa-asg-14002_st1 10.35 10.75 10.04 10.380.35 100 9.13 9.15 hsa-asg-14 hsa-asg-14_st1 4.99 4.94 5.42 5.12 0.26100 2.51 4.14 hsa-asg-13988 hsa-asg-13988_st1 2.38 2.68 2.93 2.66 0.28 04.98 6.90 hsa-asg-13970 hsa-asg-13970_st2 8.15 8.73 8.61 8.50 0.31 1007.93 7.44 hsa-asg-13966 hsa-asg-13966_st2 11.28 10.45 10.83 10.85 0.42100 9.29 9.62 hsa-asg-13933 hsa-asg-13933_st2 10.30 9.91 9.55 9.92 0.38100 8.00 8.74 hsa-asg-13931 hsa-asg-13931_st2 7.60 5.34 6.72 6.55 1.1466.66 8.64 7.61 hsa-asg-1389 hsa-asg-1389_st2 13.17 13.06 12.95 13.060.11 100 13.67 13.61 hsa-asg-13882 hsa-asg-13882_st1 1.40 0.40 1.37 1.060.57 0 2.08 1.86 hsa-asg-13870 hsa-asg-13870_st2 10.00 10.70 10.45 10.380.35 100 9.96 9.85 hsa-asg-13851 hsa-asg-13851_st1 3.63 1.79 1.65 2.361.11 0 5.34 5.61 hsa-asg-13819 hsa-asg-13819_st1 3.00 2.99 2.65 2.880.20 0 2.08 1.86 hsa-asg-13764 hsa-asg-13764_st1 8.97 7.95 8.77 8.560.54 33.33 9.64 10.19 hsa-asg-13736 hsa-asg-13736_st2 9.46 9.04 9.659.38 0.31 100 8.87 8.80 hsa-asg-13730 hsa-asg-13730_st2 1.78 1.48 1.371.54 0.21 0 3.66 5.86 hsa-asg-13659 hsa-asg-13659_st2 9.82 10.04 10.029.96 0.12 100 9.65 9.24 hsa-asg-13646 hsa-asg-13646_st1 8.57 8.58 9.358.83 0.45 100 5.47 6.55 hsa-asg-13620 hsa-asg-13620_st1 12.85 12.6012.60 12.69 0.14 100 13.20 12.88 hsa-asg-13611 hsa-asg-13611_st2 4.243.26 5.62 4.37 1.19 0 6.91 6.09 hsa-asg-13606 hsa-asg-13606_st2 10.7811.55 10.81 11.05 0.44 100 12.17 11.74 hsa-asg-13501 hsa-asg-13501_st14.80 3.68 5.42 4.63 0.89 0 6.56 7.42 hsa-asg-13500 hsa-asg-13500_st19.27 9.05 9.41 9.24 0.18 0 10.23 9.80 hsa-asg-13498 hsa-asg-13498_st17.36 7.56 6.64 7.18 0.48 0 8.57 8.79 hsa-asg-13450 hsa-asg-13450_st13.99 2.29 3.87 3.38 0.95 0 5.82 7.05 hsa-asg-13444 hsa-asg-13444_st22.10 2.05 1.65 1.93 0.25 0 3.83 4.80 hsa-asg-13436 hsa-asg-13436_st17.21 8.28 7.87 7.79 0.54 100 6.58 5.20 hsa-asg-13404 hsa-asg-13404_st26.80 3.26 2.80 4.29 2.19 33.33 1.83 0.27 hsa-asg-13387 hsa-asg-13387_st17.88 8.31 9.12 8.44 0.63 66.66 9.51 9.43 hsa-asg-1337 hsa-asg-1337_st18.73 8.76 8.18 8.56 0.33 100 6.44 6.99 hsa-asg-13353 hsa-asg-13353_st14.31 3.20 5.96 4.49 1.39 0 9.28 8.16 hsa-asg-13349 hsa-asg-13349_st24.16 3.26 4.69 4.04 0.73 66.66 2.69 3.02 hsa-asg-13334 hsa-asg-13334_st25.14 5.85 5.68 5.56 0.37 33.33 3.75 4.17 hsa-asg-13320 hsa-asg-13320_st15.75 4.90 5.19 5.28 0.43 66.66 3.66 4.35 hsa-asg-13306 hsa-asg-13306_st213.07 12.92 12.80 12.93 0.14 100 12.58 12.37 hsa-asg-13304hsa-asg-13304_st2 7.39 7.73 6.70 7.27 0.53 100 3.66 2.37 hsa-asg-13303hsa-asg-13303_st1 3.45 2.05 1.90 2.47 0.86 0 6.98 4.65 hsa-asg-13294hsa-asg-13294_st2 5.40 5.28 6.02 5.57 0.40 33.33 4.52 4.51 hsa-asg-13279hsa-asg-13279_st1 8.30 8.39 8.92 8.54 0.33 100 6.33 5.05 hsa-asg-13270hsa-asg-13270_st2 6.78 6.13 3.56 5.49 1.70 66.66 2.99 1.74 hsa-asg-13260hsa-asg-13260_st1 11.11 10.42 11.00 10.85 0.37 100 10.27 9.97hsa-asg-1324 hsa-asg-1324_st2 4.38 4.33 4.69 4.47 0.20 66.66 3.13 2.76hsa-asg-13237 hsa-asg-13237_st1 10.10 9.92 10.13 10.05 0.11 100 9.229.07 hsa-asg-13230 hsa-asg-13230_st2 7.32 6.24 8.03 7.20 0.90 100 5.023.02 hsa-asg-13203 hsa-asg-13203_st1 3.69 4.14 4.65 4.16 0.48 0 9.118.17 hsa-asg-13166 hsa-asg-13166_st2 6.42 3.48 5.30 5.07 1.48 66.66 2.512.08 hsa-asg-13143 hsa-asg-13143_st2 15.11 14.88 14.67 14.89 0.22 10014.41 14.27 hsa-asg-13097 hsa-asg-13097_st1 8.94 9.60 8.91 9.15 0.39 1005.62 1.86 hsa-asg-13091 hsa-asg-13091_st2 8.85 8.89 8.57 8.77 0.17 1009.99 9.72 hsa-asg-13079 hsa-asg-13079_st2 11.42 11.62 11.33 11.46 0.15100 11.68 11.76 hsa-asg-1306 hsa-asg-1306_st2 6.27 7.43 6.62 6.77 0.59 07.80 8.28 hsa-asg-13051 hsa-asg-13051_st1 3.32 4.21 4.53 4.02 0.63 33.332.99 2.28 hsa-asg-12996 hsa-asg-12996_st1 9.48 9.07 9.35 9.30 0.21 1008.60 8.67 hsa-asg-12973 hsa-asg-12973_st2 6.18 5.48 6.79 6.15 0.65 1003.91 5.47 hsa-asg-12946 hsa-asg-12946_st1 11.39 11.18 11.37 11.31 0.12100 12.14 11.74 hsa-asg-12943 hsa-asg-12943_st2 4.57 4.14 4.61 4.44 0.2666.66 3.57 2.89 hsa-asg-12941 hsa-asg-12941_st1 8.00 8.37 8.18 8.18 0.19100 8.79 9.46 hsa-asg-12937 hsa-asg-12937_st2 11.61 11.84 11.62 11.690.13 100 11.26 11.38 hsa-asg-12930 hsa-asg-12930_st1 0.97 1.14 1.65 1.260.35 0 4.57 1.86 hsa-asg-12919 hsa-asg-12919_st2 12.71 12.70 12.53 12.650.10 100 13.37 13.11 hsa-asg-12897 hsa-asg-12897_st1 7.63 8.03 7.87 7.850.20 0 7.36 7.00 hsa-asg-12880 hsa-asg-12880_st1 9.33 9.66 9.48 9.490.17 33.33 10.65 10.10 hsa-asg-12842 hsa-asg-12842_st1 2.61 2.99 5.323.64 1.47 33.33 6.97 5.93 hsa-asg-12821 hsa-asg-12821_st1 6.34 5.31 5.455.70 0.56 100 4.36 4.30 hsa-asg-12807 hsa-asg-12807_st2 5.06 5.25 5.625.31 0.29 33.33 1.83 4.71 hsa-asg-12799 hsa-asg-12799_st1 8.94 8.95 8.218.70 0.43 33.33 8.24 6.62 hsa-asg-12775 hsa-asg-12775_st1 11.21 11.1711.14 11.18 0.03 100 12.17 11.83 hsa-asg-12746 hsa-asg-12746_st1 4.695.05 5.25 5.00 0.28 100 4.12 2.76 hsa-asg-12734 hsa-asg-12734_st1 8.268.90 8.07 8.41 0.44 100 7.57 6.92 hsa-asg-12653 hsa-asg-12653_st1 7.077.56 7.34 7.33 0.24 100 5.02 5.86 hsa-asg-12605 hsa-asg-12605_st2 8.989.58 8.96 9.17 0.35 100 7.31 8.35 hsa-asg-12602 hsa-asg-12602_st1 2.382.29 2.31 2.33 0.04 0 1.54 1.62 hsa-asg-12595 hsa-asg-12595_st2 8.278.80 8.05 8.37 0.38 100 9.57 9.43 hsa-asg-12581 hsa-asg-12581_st1 10.5110.62 10.52 10.55 0.06 100 11.32 11.19 hsa-asg-12503 hsa-asg-12503_st11.78 3.26 4.23 3.09 1.24 0 4.61 5.02 hsa-asg-12485 hsa-asg-12485_st24.45 5.05 3.80 4.43 0.63 66.66 3.13 3.24 hsa-asg-12464 hsa-asg-12464_st12.38 2.49 2.49 2.45 0.07 0 1.83 2.28 hsa-asg-12443 hsa-asg-12443_st13.32 3.85 3.80 3.65 0.29 100 2.69 2.45 hsa-asg-12441 hsa-asg-12441_st110.28 10.19 9.82 10.10 0.24 100 11.08 10.84 hsa-asg-1243hsa-asg-1243_st1 6.79 6.40 7.03 6.74 0.32 0 7.67 7.82 hsa-asg-12424hsa-asg-12424_st2 10.64 10.54 10.07 10.41 0.30 100 9.37 9.23hsa-asg-12421 hsa-asg-12421_st1 8.77 8.30 9.65 8.91 0.69 0 9.94 10.18hsa-asg-12403 hsa-asg-12403_st2 5.48 4.98 4.98 5.15 0.29 100 4.57 4.35hsa-asg-12386 hsa-asg-12386_st1 7.87 6.87 7.75 7.49 0.55 0 8.52 8.65hsa-asg-12331 hsa-asg-12331_st1 4.85 6.91 6.49 6.08 1.09 66.66 7.93 7.67hsa-asg-12330 hsa-asg-12330_st1 5.86 4.55 5.16 5.19 0.66 33.33 4.12 4.46hsa-asg-1233 hsa-asg-1233_st1 11.24 10.87 10.89 11.00 0.21 33.33 11.4011.71 hsa-asg-12309 hsa-asg-12309_st1 10.83 10.89 10.73 10.81 0.08 10010.00 9.96 hsa-asg-12302 hsa-asg-12302_st1 9.13 9.52 9.59 9.41 0.2533.33 8.05 8.91 hsa-asg-12297 hsa-asg-12297_st2 10.83 10.88 11.16 10.960.18 100 9.53 9.05 hsa-asg-12289 hsa-asg-12289_st1 11.81 11.96 11.7511.84 0.11 100 12.42 12.11 hsa-asg-12278 hsa-asg-12278_st2 10.19 10.2610.76 10.40 0.31 100 9.81 9.88 hsa-asg-1227 hsa-asg-1227_st1 4.99 5.895.16 5.35 0.48 33.33 6.55 6.22 hsa-asg-12247 hsa-asg-12247_st2 12.3012.43 12.27 12.33 0.08 100 12.13 12.18 hsa-asg-12244 hsa-asg-12244_st19.41 8.74 8.57 8.91 0.45 100 7.59 7.67 hsa-asg-12119 hsa-asg-12119_st26.76 6.03 6.59 6.46 0.39 100 2.08 3.13 hsa-asg-12096 hsa-asg-12096_st23.80 3.48 3.47 3.58 0.18 0 3.36 2.61 hsa-asg-12058 hsa-asg-12058_st15.24 6.72 5.76 5.91 0.75 33.33 7.09 7.85 hsa-asg-12049 hsa-asg-12049_st210.45 10.55 10.46 10.49 0.06 100 9.48 9.47 hsa-asg-12027hsa-asg-12027_st1 4.90 4.33 4.73 4.65 0.29 33.33 2.85 3.60 hsa-asg-1201hsa-asg-1201_st2 9.63 9.66 9.89 9.73 0.14 100 10.24 10.49 hsa-asg-11997hsa-asg-11997_st1 5.59 5.89 4.88 5.45 0.52 33.33 4.05 5.05 hsa-asg-11982hsa-asg-11982_st1 9.23 10.26 9.28 9.59 0.58 100 7.95 8.73 hsa-asg-11975hsa-asg-11975_st1 9.98 10.04 9.91 9.98 0.06 100 10.61 10.25hsa-asg-11962 hsa-asg-11962_st1 8.73 9.28 8.98 9.00 0.28 100 8.58 8.55hsa-asg-11956 hsa-asg-11956_st1 9.36 9.02 9.25 9.21 0.17 100 8.71 8.42hsa-asg-11946 hsa-asg-11946_st1 10.46 10.88 10.39 10.58 0.26 100 9.8110.13 hsa-asg-11940 hsa-asg-11940_st1 9.01 9.12 8.90 9.01 0.11 100 8.608.57 hsa-asg-11912 hsa-asg-11912_st2 7.97 8.10 7.49 7.85 0.32 100 3.753.72 hsa-asg-1189 hsa-asg-1189_st1 9.54 9.97 9.58 9.70 0.24 100 7.619.05 hsa-asg-11877 hsa-asg-11877_st1 7.44 7.98 7.13 7.52 0.43 33.33 7.076.75 hsa-asg-11872 hsa-asg-11872_st1 6.38 3.93 3.87 4.72 1.43 33.33 1.832.69 hsa-asg-11827 hsa-asg-11827_st1 2.10 3.48 2.65 2.74 0.69 0 5.253.75 hsa-asg-11818 hsa-asg-11818_st2 11.87 12.21 11.89 11.99 0.19 10011.33 11.14 hsa-asg-11766 hsa-asg-11766_st1 3.69 3.37 3.38 3.48 0.18 03.83 3.83 hsa-asg-11758 hsa-asg-11758_st2 12.83 12.81 12.56 12.73 0.15100 13.12 13.01 hsa-asg-11698 hsa-asg-11698_st2 10.99 11.24 10.97 11.070.15 100 11.99 11.58 hsa-asg-11686 hsa-asg-11686_st2 1.40 1.48 1.37 1.420.06 0 2.31 1.86 hsa-asg-11643 hsa-asg-11643_st1 4.08 4.33 3.80 4.070.27 33.33 2.69 2.89 hsa-asg-11639 hsa-asg-11639_st2 3.99 4.00 4.77 4.250.45 33.33 3.57 2.61 hsa-asg-11635 hsa-asg-11635_st1 3.69 1.48 2.12 2.431.14 0 3.47 6.03 hsa-asg-11617 hsa-asg-11617_st2 9.91 9.97 9.59 9.830.21 100 10.06 10.59 hsa-asg-11612 hsa-asg-11612_st1 9.65 8.56 9.66 9.290.63 100 7.82 7.99 hsa-asg-11608 hsa-asg-11608_st1 11.02 11.12 10.9811.04 0.08 100 9.68 10.28 hsa-asg-11606 hsa-asg-11606_st1 11.76 12.0511.79 11.87 0.16 100 11.39 11.17 hsa-asg-11579 hsa-asg-11579_st2 4.905.45 5.54 5.30 0.35 100 2.51 2.89 hsa-asg-1157 hsa-asg-1157_st1 8.729.50 8.69 8.97 0.46 100 7.55 7.14 hsa-asg-11563 hsa-asg-11563_st2 10.1311.19 10.58 10.63 0.54 100 9.65 9.44 hsa-asg-11535 hsa-asg-11535_st213.98 13.99 13.79 13.92 0.12 100 13.64 13.43 hsa-asg-11513hsa-asg-11513_st1 10.14 10.06 10.08 10.09 0.04 100 9.28 9.57hsa-asg-11503 hsa-asg-11503_st1 9.42 8.62 9.22 9.09 0.41 33.33 8.74 8.38hsa-asg-1140 hsa-asg-1140_st2 7.94 8.12 8.47 8.18 0.27 100 7.58 7.60hsa-asg-11389 hsa-asg-11389_st1 11.85 11.83 11.77 11.82 0.04 100 12.4812.24 hsa-asg-11388 hsa-asg-11388_st1 7.74 7.72 7.73 7.73 0.01 100 6.977.26 hsa-asg-11384 hsa-asg-11384_st1 1.78 1.79 2.12 1.89 0.19 0 2.083.13 hsa-asg-11381 hsa-asg-11381_st2 8.76 9.20 9.60 9.19 0.42 100 7.928.57 hsa-asg-11336 hsa-asg-11336_st2 10.27 10.53 10.67 10.49 0.20 10010.89 10.85 hsa-asg-11326 hsa-asg-11326_st2 7.64 8.33 8.35 8.11 0.40 1007.12 7.14 hsa-asg-11246 hsa-asg-11246_st2 1.78 1.48 1.65 1.64 0.15 02.69 1.86 hsa-asg-11228 hsa-asg-11228_st1 9.92 10.26 9.97 10.05 0.19 1009.62 9.65 hsa-asg-11196 hsa-asg-11196_st1 2.38 2.29 1.90 2.19 0.26 03.75 2.76 hsa-asg-11193 hsa-asg-11193_st1 11.45 11.71 11.63 11.60 0.14100 12.26 12.27 hsa-asg-11191 hsa-asg-11191_st1 4.38 3.93 3.87 4.06 0.2833.33 3.25 2.89 hsa-asg-11174 hsa-asg-11174_st1 7.85 7.64 8.51 8.00 0.4633.33 9.32 8.70 hsa-asg-11130 hsa-asg-11130_st2 1.78 1.79 2.31 1.96 0.310 3.66 4.53 hsa-asg-11112 hsa-asg-11112_st2 10.51 11.18 10.86 10.85 0.33100 11.57 11.26 hsa-asg-11089 hsa-asg-11089_st1 9.98 9.12 9.72 9.60 0.44100 10.13 10.39 hsa-asg-11061 hsa-asg-11061_st2 2.10 2.49 2.65 2.42 0.280 6.65 3.60 hsa-asg-11056 hsa-asg-11056_st1 6.57 6.63 6.27 6.49 0.19 1006.06 5.08 hsa-asg-11002 hsa-asg-11002_st2 5.67 4.64 6.46 5.59 0.91 66.663.47 2.37 hsa-asg-10971 hsa-asg-10971_st2 9.85 9.54 10.21 9.87 0.34 1009.47 8.83 hsa-asg-10957 hsa-asg-10957_st1 0.07 0.78 2.12 0.99 1.04 04.47 4.82 hsa-asg-10932 hsa-asg-10932_st2 5.06 4.69 5.70 5.15 0.51 33.334.18 3.38 hsa-asg-10926 hsa-asg-10926_st2 2.10 1.79 1.65 1.84 0.23 02.99 5.95 hsa-asg-10922 hsa-asg-10922_st2 7.24 5.81 6.08 6.38 0.76 1008.11 7.84 hsa-asg-10918 hsa-asg-10918_st1 7.11 7.71 6.51 7.11 0.60 1004.57 4.57 hsa-asg-10914 hsa-asg-10914_st1 3.45 3.76 4.39 3.87 0.48 33.332.51 2.08 hsa-asg-10901 hsa-asg-10901_st1 6.53 6.69 5.89 6.37 0.42 1007.24 6.90 hsa-asg-10893 hsa-asg-10893_st1 4.45 3.93 4.91 4.43 0.49 66.662.85 2.45 hsa-asg-10862 hsa-asg-10862_st2 4.24 4.64 4.61 4.50 0.23 1002.99 3.34 hsa-asg-10860 hsa-asg-10860_st2 3.17 2.99 4.12 3.43 0.61 66.661.83 2.28 hsa-asg-10810 hsa-asg-10810_st1 4.38 4.69 4.44 4.50 0.17 1003.36 3.43 hsa-asg-10807 hsa-asg-10807_st2 7.73 8.41 8.06 8.07 0.34 33.339.34 9.19 hsa-asg-10647 hsa-asg-10647_st1 4.12 2.99 1.90 3.00 1.11 05.25 6.48 hsa-asg-10639 hsa-asg-10639_st1 12.16 12.55 12.23 12.31 0.21100 11.78 11.90 hsa-asg-10638 hsa-asg-10638_st2 11.88 12.00 11.96 11.950.06 100 12.33 12.02 hsa-asg-10580 hsa-asg-10580_st1 11.38 11.04 10.4310.95 0.48 100 12.66 12.38 hsa-asg-10576 hsa-asg-10576_st1 10.14 11.1811.09 10.80 0.58 100 9.43 9.58 hsa-asg-10556 hsa-asg-10556_st1 3.45 3.853.64 3.65 0.20 0 5.22 4.59 hsa-as -10512 hsa-asg-10512_st1 9.91 9.9910.04 9.98 0.07 100 10.56 10.38 hsa-asg-10510 hsa-asg-10510_st2 5.674.78 5.91 5.45 0.59 33.33 6.54 7.63 hsa-asg-10481 hsa-asg-10481_st1 7.647.06 6.80 7.17 0.43 100 6.37 5.35 hsa-asg-10480 hsa-asg-10480_st1 2.501.79 3.28 2.52 0.75 0 6.42 3.79 hsa-asg-10411 hsa-asg-10411_st1 7.476.39 5.66 6.51 0.91 66.66 10.49 10.07 hsa-asg-1038 hsa-asg-1038_st2 3.994.00 3.56 3.85 0.25 0 8.03 6.60 hsa-asg-10371 hsa-asg-10371_st2 3.002.49 3.17 2.89 0.35 0 1.83 1.05 hsa-asg-10273 hsa-asg-10273_st1 2.382.29 3.94 2.87 0.93 0 6.44 6.06 hsa-asg-10263 hsa-asg-10263_st2 4.904.44 4.34 4.56 0.30 100 2.31 2.28 hsa-asg-1026 hsa-asg-1026_st2 5.045.45 5.13 5.21 0.22 100 5.47 5.58 hsa-asg-10256 hsa-asg-10256_st1 2.102.84 1.37 2.11 0.74 0 3.36 4.84 hsa-asg-10247 hsa-asg-10247_st1 4.312.84 3.56 3.57 0.73 33.33 2.08 2.45 hsa-asg-10235 hsa-asg-10235_st2 4.854.00 4.12 4.32 0.46 33.33 6.25 6.13 hsa-asg-10231 hsa-asg-10231_st1 5.334.98 4.77 5.03 0.28 0 2.08 3.86 hsa-asg-10229 hsa-asg-10229_st2 5.384.60 4.44 4.81 0.51 66.66 3.66 2.61 hsa-asg-10222 hsa-asg-10222_st2 5.204.69 4.88 4.92 0.26 100 3.57 3.75 hsa-asg-10199 hsa-asg-10199_st1 10.0910.28 10.46 10.28 0.19 100 9.77 9.55 hsa-asg-10197 hsa-asg-10197_st210.47 10.25 9.89 10.20 0.29 100 9.22 9.74 hsa-asg-10195hsa-asg-10195_st2 9.87 10.00 9.61 9.83 0.20 100 8.13 8.97 hsa-asg-10181hsa-asg-10181_st1 8.53 8.20 8.86 8.53 0.33 100 10.02 9.80 hsa-asg-1018hsa-asg-1018_st2 7.14 7.82 8.36 7.77 0.61 100 6.18 6.90 hsa-asg-10170hsa-asg-10170_st2 5.08 5.97 6.71 5.92 0.82 66.66 3.66 4.17 hsa-asg-10158hsa-asg-10158_st2 5.20 6.46 7.39 6.35 1.10 66.66 3.83 4.37 hsa-asg-10141hsa-asg-10141_st1 1.78 0.40 1.37 1.18 0.71 0 2.51 4.02 hsa-asg-10081hsa-asg-10081_st1 3.99 4.60 3.06 3.88 0.77 33.33 1.54 2.08 hsa-asg-10076hsa-asg-10076_st1 10.16 10.05 10.50 10.24 0.24 100 9.46 9.82hsa-asg-10037 hsa-asg-10037_st1 4.51 4.39 4.65 4.52 0.13 0 6.85 5.38hsa-asg-1001 hsa-asg-1001_st1 10.43 10.77 10.64 10.61 0.17 100 9.38 9.27hsa-miR-140 hsa-asg-14239 8.56 7.97 9.14 8.56 0.58 100 6.71 2.37hsa-miR-29b hsa-asg-14229_st2 11.64 11.42 11.99 11.68 0.29 100 9.51 8.26hsa-miR-329 hsa-asg-13589_st2 7.90 2.84 6.69 5.81 2.64 66.66 1.83 1.62hsa-miR-329 hsa-asg-13204_st2 8.02 5.31 6.37 6.57 1.36 100 3.66 2.08hsa-miR-365 hsa-asg-13156_st1 6.30 6.79 8.37 7.15 1.08 100 1.22 2.08hsa-miR-369- hsa-asg-1521_st1 6.41 4.94 6.88 6.07 1.01 66.66 2.51 1.355p hsa-miR-376a hsa-asg-10049_st1 8.08 6.54 7.85 7.49 0.83 100 3.66 2.76hsa-miR-376a hsa-asg-14234_st1 6.61 4.55 6.72 5.96 1.22 100 1.83 1.62hsa-miR-421 hsa-asg-5033_st2 7.09 7.30 8.00 7.46 0.47 100 1.22 3.86hsa-miR-451 hsa-asg-13195_st2 14.10 13.96 14.24 14.10 0.14 100 9.36 9.26hsa-miR-497 hsa-miR43_st1 12.90 12.34 12.47 12.57 0.29 100 8.05 8.41hsa-miR-517a hsa-miR-517b_st1 7.65 6.77 6.29 6.91 0.69 100 2.31 1.86hsa-miR-551b hsa-miR167_st2 8.16 8.63 9.04 8.61 0.44 100 0.86 3.47hsa-miR-99b hsa-asg-14196_st2 11.33 11.54 11.57 11.48 0.13 66.66 8.488.15 hsa-miR- hsa-asg-14140_st1 14.32 13.89 13.98 14.07 0.23 100 10.499.76 199a* hsa-miR-224 hsa-asg-14247_st1 11.48 12.27 12.00 11.92 0.40100 5.02 4.65 NBr vs LNneg Micro LNneg Fold RNA 3 4 Mean SD % ttestLog2Diff Change hsa-let-7a 13.24 13.22 13.01 0.30 100 3.44E−04 1.74 3hsa-let-7b 11.57 11.57 11.27 0.40 100 2.43E−04 2.28 5 hsa-let-7c 12.4412.53 12.27 0.26 100 5.79E−05 2.11 4 hsa-let-7d 12.65 12.66 12.40 0.31100 1.69E−04 1.97 4 hsa-let-7e 10.14 10.40 10.09 0.34 100 9.27E−05 2.355 hsa-let-7f 12.70 12.91 12.35 0.54 100 1.56E−03 2.05 4 hsa-let-7g 12.3412.33 12.07 0.44 100 1.71E−03 1.72 3 hsa-let-7i 10.84 11.37 10.92 0.40100 2.57E−04 2.41 5 hsa-miR-1 2.34 4.12 2.47 1.18 25 9.54E−03 6.54 93hsa-miR-100 10.11 10.00 9.76 0.34 100 2.72E−05 3.27 10 hsa-miR-101 4.924.18 5.25 0.89 75 1.14E−03 3.84 14 hsa-miR-103 11.22 11.27 11.02 0.26100 3.22E−05 2.33 5 hsa-miR-106a 9.68 10.20 9.73 0.34 100 1.11E−04 2.948 hsa-miR-106b 8.17 7.51 7.44 0.61 100 4.15E−04 3.63 12 hsa-miR-10710.93 11.04 10.71 0.34 100 7.31E−05 2.46 6 hsa-miR-10a 8.27 8.56 8.300.19 100 6.66E−07 3.50 11 hsa-miR-10b 9.93 10.39 9.95 0.38 100 5.10E−052.93 8 hsa-miR-125a 10.35 9.98 10.03 0.22 100 2.75E−05 2.29 5hsa-miR-125b 11.62 12.09 11.49 0.58 100 5.73E−04 2.83 7 hsa-miR-12612.27 12.39 12.20 0.28 100 3.48E−05 2.36 5 hsa-miR-126* 1.84 1.94 2.260.44 0 5.29E−04 3.36 10 hsa-miR-127 4.65 5.23 4.71 0.39 0 7.37E−04 4.5323 hsa-miR-128a 6.91 6.64 6.19 0.73 100 7.88E−04 3.28 10 hsa-miR-128b7.50 7.65 7.04 0.75 75 1.63E−03 2.75 7 hsa-miR-130a 8.60 8.45 8.35 0.25100 1.22E−04 2.91 8 hsa-miR-130b 5.93 6.29 6.16 0.31 75 1.09E−03 1.73 3hsa-miR-132 5.81 6.08 5.06 1.92 0 2.23E−02 4.01 16 hsa-miR-133a 1.532.92 2.16 0.75 0 4.37E−02 5.00 32 hsa-miR-134 7.94 7.41 7.68 0.32 504.88E−02 0.71 2 hsa-miR-137 2.11 1.94 2.05 0.08 0 4.52E−02 0.28 1hsa-miR-139 8.02 6.99 6.89 1.36 75 6.32E−03 3.63 12 hsa-miR-140 1.981.46 1.87 0.61 0 4.21E−05 5.83 57 hsa-miR-141 1.19 2.79 1.89 0.67 01.04E−03 7.34 162 hsa-miR-143 9.75 10.53 10.01 0.35 100 6.33E−06 4.10 17hsa-miR-145 12.61 12.70 12.45 0.23 100 2.88E−05 2.10 4 hsa-miR-146b 9.8510.06 9.62 0.42 100 1.47E−02 1.87 4 hsa-miR-148a 8.84 8.70 8.46 0.57 1002.84E−04 3.91 15 hsa-miR-148b 5.26 6.53 5.31 0.87 100 3.40E−04 4.46 22hsa-miR-150 14.14 13.62 13.74 0.31 100 3.40E−04 −1.63 3 hsa-miR-151 6.137.08 6.52 0.48 100 1.76E−04 3.19 9 hsa-miR-152 7.47 7.63 7.28 0.32 1006.30E−06 4.35 20 hsa-miR-154 1.53 3.15 2.35 0.83 0 1.87E−03 4.59 24hsa-miR-154* 3.19 2.79 2.83 0.54 0 4.39E−04 2.92 8 hsa-miR-155 10.9711.11 10.72 0.49 100 3.68E−03 −1.54 3 hsa-miR-15a 8.67 9.05 8.66 0.41100 5.73E−05 3.42 11 hsa-miR-15b 9.64 9.32 9.12 0.55 100 8.52E−04 2.49 6hsa-miR-16 12.53 12.53 12.31 0.26 100 3.98E−05 2.10 4 hsa-miR-17- 2.745.76 4.96 1.69 25 4.98E−02 2.88 7 3p hsa-miR-17- 9.78 9.88 9.59 0.32 1002.89E−04 2.78 7 5p hsa-miR-181a 10.11 9.38 9.49 0.43 100 5.19E−03 1.72 3hsa-miR- 1.53 4.85 3.55 1.82 0 2.03E−02 3.62 12 181a* hsa-miR-181b 8.087.62 7.63 0.32 100 1.51E−02 1.07 2 hsa-miR-181d 4.23 3.75 4.22 0.40 251.99E−04 3.07 8 hsa-miR-182 4.13 2.79 3.70 0.63 25 3.10E−05 5.77 55hsa-miR-185 6.97 7.23 6.52 0.99 100 3.14E−03 3.54 12 hsa-miR-186 1.534.33 2.82 1.34 25 9.85E−03 3.47 11 hsa-miR-189 0.61 1.94 1.17 0.68 01.47E−04 5.19 36 hsa-miR-18a 3.12 5.12 3.21 1.35 25 2.23E−02 3.22 9hsa-miR-18b 2.98 2.33 2.57 0.77 0 2.30E−03 3.50 11 hsa-miR-191 11.6611.63 11.38 0.32 100 4.95E−04 1.68 3 hsa-miR-192 4.35 6.63 5.92 1.15 752.81E−02 2.18 5 hsa-miR-193a 5.66 5.38 5.82 0.38 50 4.02E−05 3.50 11hsa-miR-193b 8.93 8.20 8.08 0.77 25 1.12E−03 3.71 13 hsa-miR-194 6.516.01 5.86 0.54 100 1.46E−03 2.59 6 hsa-miR-195 10.48 10.73 10.33 0.41100 4.17E−05 3.69 13 hsa-miR-196b 2.55 1.94 2.71 0.84 0 2.67E−02 3.27 10hsa-miR-199a 9.48 9.31 9.42 0.23 100 3.31E−05 3.15 9 hsa-miR- 10.6410.87 10.38 0.71 100 3.50E−04 3.73 13 199a* hsa-miR-199b 5.60 5.01 4.841.09 75 1.44E−04 7.00 128 hsa-miR-19a 3.54 3.15 3.08 0.70 0 5.77E−055.89 59 hsa-miR-19b 8.84 8.36 8.13 0.65 100 1.55E−04 4.41 21hsa-miR-200a 2.55 2.79 2.47 0.29 0 2.49E−04 7.44 174 hsa-miR-200b 2.553.52 2.61 0.68 0 8.44E−05 8.96 499 hsa-miR-200c 5.74 7.26 5.98 0.92 1006.12E−04 6.02 65 hsa-miR-203 2.45 2.65 2.95 0.47 0 3.08E−04 4.23 19hsa-miR-205 3.25 6.93 3.43 2.54 25 1.22E−03 10.03 1048 hsa-miR-20a 9.529.21 9.12 0.40 100 1.66E−04 3.12 9 hsa-miR-20b 6.40 7.83 7.15 0.61 1006.29E−04 3.32 10 hsa-miR-21 10.51 10.91 10.49 0.53 100 1.42E−04 3.29 10hsa-miR-214 9.16 9.01 9.02 0.39 25 7.08E−03 1.12 2 hsa-miR-215 2.34 3.342.62 0.49 0 7.79E−04 3.74 13 hsa-miR-218 2.90 1.94 2.56 1.18 25 3.75E−046.10 68 hsa-miR-22 9.09 9.27 9.08 0.16 100 5.38E−07 4.46 22 hsa-miR-22110.77 10.32 10.21 0.50 100 9.55E−04 2.32 5 hsa-miR-222 10.88 10.66 10.530.41 75 2.43E−03 1.61 3 hsa-miR-223 9.41 10.19 9.58 0.41 100 1.42E−032.30 5 hsa-miR-224 4.29 6.19 5.32 0.78 75 4.95E−05 6.43 86 hsa-miR-23a12.52 12.63 12.19 0.46 100 3.41E−04 2.34 5 hsa-miR-23b 12.18 12.31 11.890.42 100 2.14E−04 2.37 5 hsa-miR-24 10.89 10.97 10.56 0.43 100 7.03E−053.06 8 hsa-miR-25 10.03 9.77 9.82 0.15 100 1.25E−06 2.51 6 hsa-miR-26a13.57 13.37 13.15 0.41 100 2.13E−03 1.50 3 hsa-miR-26b 9.45 10.73 9.860.68 100 8.57E−04 3.03 8 hsa-miR-27a 8.45 9.08 8.65 0.30 100 1.25E−054.11 17 hsa-miR-27b 8.56 9.15 8.47 0.50 100 3.01E−05 4.27 19 hsa-miR-287.07 8.72 8.02 0.74 100 9.78E−04 3.09 9 hsa-miR-299- 2.90 2.50 2.50 0.340 1.07E−04 3.73 13 5p hsa-miR-29a 11.84 12.05 11.83 0.27 100 2.47E−041.55 3 hsa-miR-29b 9.35 10.04 9.38 0.76 100 6.13E−03 2.23 5 hsa-miR-29c7.25 9.01 8.29 1.03 100 1.17E−02 2.50 6 hsa-miR-30a- 5.55 6.75 5.78 0.7875 1.55E−04 4.76 27 3p hsa-miR-30a- 9.73 10.09 9.76 0.22 100 8.94E−063.55 12 5p hsa-miR-30b 9.52 10.22 9.61 0.50 100 1.97E−04 3.20 9hsa-miR-30c 11.13 10.79 10.71 0.39 100 1.04E−04 2.82 7 hsa-miR-30d 10.5410.50 10.36 0.20 100 2.82E−05 2.69 6 hsa-miR-30e- 6.33 6.55 6.73 0.62100 1.08E−03 2.75 7 3p hsa-miR-30e- 8.19 8.22 8.17 0.40 100 7.24E−053.57 12 5p hsa-miR-31 8.00 6.53 6.79 0.97 75 2.83E−02 2.61 6 hsa-miR-32010.18 9.91 9.86 0.25 75 2.29E−03 0.85 2 hsa-miR-329 2.11 4.81 3.29 1.1325 1.15E−02 3.28 10 hsa-miR-335 5.88 7.29 6.21 0.85 100 4.25E−05 6.82113 hsa-miR-34a 6.98 7.40 6.56 0.75 100 4.61E−03 2.33 5 hsa-miR-34b 2.342.65 2.44 0.29 0 3.10E−02 2.62 6 hsa-miR-361 9.84 9.66 9.09 0.77 1005.33E−03 2.19 5 hsa-miR-362 6.08 5.04 5.73 0.48 25 3.02E−03 1.68 3hsa-miR-363 6.45 7.05 6.68 0.40 100 2.34E−03 2.36 5 hsa-miR-365 1.843.44 2.79 0.68 0 8.86E−04 4.25 19 hsa-miR-368 2.34 3.52 2.19 0.99 251.30E−04 7.94 246 hsa-miR-369- 1.53 1.71 1.67 0.13 0 4.10E−04 5.41 42 5phsa-miR-375 0.23 5.42 2.13 2.27 0 1.94E−02 4.59 24 hsa-miR-376a 3.312.92 2.84 0.43 0 3.68E−03 4.06 17 hsa-miR-377 4.44 3.89 3.00 1.36 252.03E−02 3.43 11 hsa-miR-379 6.13 4.81 4.50 1.32 25 3.17E−03 4.45 22hsa-miR-381 0.81 2.33 2.42 1.38 0 8.79E−03 4.25 19 hsa-miR-382 5.12 5.095.09 0.42 0 6.58E−04 3.49 11 hsa-miR-409- 4.06 3.25 3.35 0.74 0 8.68E−032.95 8 5p hsa-miR-410 2.34 2.33 2.56 0.45 0 3.29E−02 2.55 6 hsa-miR-4110.42 2.50 1.54 1.05 0 1.69E−04 6.97 126 hsa-miR-421 2.82 1.71 2.38 0.700 1.69E−04 5.15 35 hsa-miR-422a 7.74 7.47 7.11 0.63 0 1.87E−04 4.19 18hsa-miR-422b 8.56 7.80 8.42 0.44 100 8.12E−05 3.88 15 hsa-miR-423 7.698.47 7.75 0.57 25 3.61E−03 1.91 4 hsa-miR-424 2.74 3.15 2.33 0.73 08.66E−04 5.71 52 hsa-miR-425- 8.63 8.23 8.36 0.21 100 2.58E−05 2.10 4 5phsa-miR-429 1.84 3.82 2.77 1.09 0 8.85E−04 5.28 39 hsa-miR-432 3.98 4.714.10 0.44 0 9.04E−04 2.58 6 hsa-miR-451 8.24 6.92 7.85 0.71 100 5.71E−055.68 51 hsa-miR-452 5.35 4.67 4.96 0.65 50 3.05E−05 5.58 48 hsa-miR-452*5.83 2.33 2.94 2.13 25 1.44E−02 4.63 25 hsa-miR-486 7.23 4.85 5.95 1.090 7.35E−04 5.37 41 hsa-miR-487a 4.65 4.38 4.88 0.52 100 4.21E−03 2.30 5hsa-miR-487b 5.07 2.65 4.14 1.06 75 2.26E−03 4.76 27 hsa-miR-489 1.982.79 2.38 0.70 0 9.64E−04 4.38 21 hsa-miR-493- 0.81 1.71 1.48 0.47 01.28E−02 3.41 11 5p hsa-miR-495 1.84 2.79 2.40 0.43 0 9.20E−05 5.80 56hsa-miR-497 8.76 8.41 8.26 0.40 25 4.70E−05 4.10 17 hsa-miR-500 7.197.40 7.21 0.13 100 8.65E−04 0.82 2 hsa-miR-503 7.29 7.03 6.65 0.74 02.37E−02 1.69 3 hsa-miR-505 6.57 5.51 5.14 1.14 25 4.51E−02 1.87 4hsa-miR-516- 4.58 2.15 2.66 1.29 0 3.45E−02 2.70 7 5p hsa-miR-517a 2.981.94 2.92 0.80 0 6.63E−04 3.63 12 hsa-miR-542- 3.43 4.23 3.73 0.80 503.73E−02 2.21 5 3p hsa-miR-542- 0.81 0.89 1.55 0.89 0 1.36E−03 4.03 165p hsa-miR-551b 2.34 3.25 2.33 0.70 0 9.90E−05 5.91 60 hsa-miR-557 8.598.73 8.60 0.12 100 3.90E−03 0.96 2 hsa-miR-565 8.68 10.03 9.79 0.74 1001.28E−02 1.83 4 hsa-miR-566 8.95 8.47 8.97 0.39 25 2.56E−03 −2.57 6hsa-miR-574 7.88 6.88 7.34 0.45 25 7.50E−04 2.13 4 hsa-miR-583 4.41 5.093.78 1.16 25 7.88E−03 3.02 8 hsa-miR-598 2.65 2.50 2.71 0.64 0 1.23E−033.23 9 hsa-miR-624 1.69 3.34 2.65 0.75 0 3.65E−02 2.11 4 hsa-miR-6287.18 6.75 7.23 0.51 100 1.91E−02 1.20 2 hsa-miR-630 2.45 3.04 2.91 0.340 3.34E−03 1.73 3 hsa-miR-655 3.54 4.91 3.68 0.85 25 7.17E−03 2.21 5hsa-miR-660 4.77 6.49 6.12 0.91 100 2.33E−03 3.21 9 hsa-miR-9* 3.19 1.942.79 0.70 0 2.45E−02 1.99 4 hsa-miR-92 11.79 11.42 11.36 0.32 1003.18E−02 0.74 2 hsa-miR-93 7.65 7.70 7.80 0.19 100 6.87E−06 3.13 9hsa-miR-96 2.34 1.94 2.16 0.18 0 2.23E−05 5.18 36 hsa-miR-98 6.81 6.056.21 0.44 100 1.12E−04 4.15 18 hsa-miR-99a 9.97 9.91 9.69 0.30 1008.36E−06 3.89 15 hsa-miR-99b 8.49 8.50 8.31 0.22 0 4.46E−06 3.03 8hsa-miR154 4.96 1.71 3.42 1.33 25 5.69E−04 6.48 89 hsa-miR144 3.05 2.792.38 0.63 0 4.82E−05 5.48 45 hsa-asg-9769 2.23 3.25 2.73 0.47 0 6.46E−042.43 5 hsa-asg-9483 8.05 7.94 8.08 0.11 0 1.26E−04 2.30 5 hsa-asg-94057.70 8.03 7.98 0.32 100 2.53E−04 3.16 9 hsa-asg-9253 6.73 6.60 6.90 0.28100 1.32E−05 −4.37 21 hsa-asg-9232 2.11 1.46 2.01 0.38 0 1.63E−04 3.18 9hsa-asg-9200 4.50 4.12 4.16 0.24 50 4.47E−04 1.79 3 hsa-asg-9112 9.749.58 9.83 0.21 100 8.86E−06 2.79 7 hsa-asg-8901 12.24 12.15 12.23 0.06100 3.22E−04 1.40 3 hsa-asg-860 2.34 2.65 2.65 0.27 0 3.55E−04 1.78 3hsa-asg-8195 7.36 7.49 7.49 0.58 100 4.07E−05 −6.27 77 hsa-asg-798510.23 10.13 10.39 0.26 100 3.26E−04 1.40 3 hsa-asg-772 8.90 8.95 8.860.14 25 5.77E−04 1.05 2 hsa-asg-7569 8.39 7.98 8.28 0.27 100 9.40E−04−1.68 3 hsa-asg-7461 6.36 6.45 6.69 0.37 100 5.87E−04 1.79 3hsa-asg-7352 9.84 10.13 9.90 0.15 100 2.21E−04 1.37 3 hsa-asg-7189 10.2410.16 10.12 0.10 100 6.29E−05 1.77 3 hsa-asg-7098 6.84 5.40 6.53 0.78100 2.77E−04 −4.89 30 hsa-asg-6957 9.26 9.58 9.70 0.35 0 3.25E−04 1.95 4hsa-asg-6932 11.81 11.58 11.64 0.24 100 2.59E−04 −1.45 3 hsa-asg-687010.65 10.35 10.50 0.16 100 8.88E−04 1.27 2 hsa-asg-680 5.75 7.15 6.910.79 100 4.84E−04 3.82 14 hsa-asg-6457 3.59 2.92 3.32 0.29 0 5.86E−041.84 4 hsa-asg-6247 9.96 10.02 9.88 0.38 100 9.38E−04 1.72 3hsa-asg-5998 8.89 8.98 9.13 0.27 75 5.94E−06 3.96 16 hsa-asg-5932 12.9212.77 12.98 0.17 100 7.04E−05 1.54 3 hsa-asg-5760 5.68 5.93 6.09 0.77100 2.90E−04 −4.29 20 hsa-asg-5021 1.98 1.46 2.03 0.47 0 1.70E−05 6.66101 hsa-asg-4557 3.90 3.44 3.55 0.28 0 2.19E−04 3.45 11 hsa-asg-45237.14 7.28 7.29 0.17 0 1.89E−04 1.51 3 hsa-asg-4515 2.98 2.15 2.38 0.41 04.54E−04 2.34 5 hsa-asg-4416 2.74 2.15 2.25 0.38 0 8.12E−04 1.86 4hsa-asg-4350 5.16 5.07 5.51 0.49 100 2.39E−04 −3.77 14 hsa-asg-4163 4.133.82 3.73 0.31 0 4.68E−04 1.54 3 hsa-asg-3998 8.44 8.22 8.37 0.27 1007.50E−05 2.84 7 hsa-asg-3811 7.32 8.42 7.77 0.48 100 9.02E−04 2.47 6hsa-asg-3604 3.73 4.18 3.65 0.41 25 6.14E−04 1.86 4 hsa-asg-3545 11.1510.69 10.99 0.21 100 2.79E−04 1.67 3 hsa-asg-3167 10.19 10.08 10.19 0.19100 9.23E−04 1.14 2 hsa-asg-3138 7.86 7.90 8.07 0.33 100 2.06E−04 1.99 4hsa-asg-2944 3.25 3.44 3.14 0.29 0 3.88E−04 1.82 4 hsa-asg-2824 11.2611.01 11.17 0.11 100 1.99E−04 −1.34 3 hsa-asg-2785 9.77 9.64 9.83 0.18100 4.65E−06 2.84 7 hsa-asg-2750 12.35 12.19 12.19 0.12 75 6.41E−04 1.122 hsa-asg-2739 10.12 9.93 10.20 0.24 100 2.23E−04 1.35 3 hsa-asg-2539.39 8.52 9.04 0.38 100 8.76E−04 1.69 3 hsa-asg-2445 7.13 7.35 7.09 0.55100 1.85E−04 −5.31 40 hsa-asg-2073 2.34 3.04 2.85 0.43 0 6.93E−04 2.06 4hsa-asg-1895 9.60 9.37 9.77 0.40 0 2.97E−04 2.34 5 hsa-asg-1514 7.167.26 7.05 0.21 0 6.35E−04 1.70 3 hsa-asg-1468 8.93 8.56 8.94 0.32 509.03E−04 1.60 3 hsa-asg-14238 2.11 2.65 2.94 0.79 0 2.46E−04 4.94 31hsa-asg-14204 8.81 8.56 8.75 0.20 25 7.11E−04 −1.12 2 hsa-asg-14186 2.112.50 2.40 0.38 0 9.89E−04 2.42 5 hsa-asg-14175 2.34 2.65 2.38 0.24 04.39E−04 3.88 15 hsa-asg-14170 2.65 2.50 2.99 0.72 0 8.75E−05 5.40 42hsa-asg-14146 2.11 3.89 2.41 1.07 0 8.66E−04 4.64 25 hsa-asg-14134 4.814.42 4.66 0.33 50 3.43E−05 4.11 17 hsa-asg-14038 9.72 9.56 9.73 0.13 502.29E−04 2.38 5 hsa-asg-13770 7.63 7.52 7.85 0.32 100 1.89E−04 1.97 4hsa-asg-13403 7.04 7.54 7.34 0.40 100 1.11E−04 2.79 7 hsa-asg-1335413.79 13.36 13.66 0.23 100 2.50E−04 −2.12 4 hsa-asg-13312 9.98 9.34 9.920.51 100 1.94E−04 −4.79 28 hsa-asg-13258 5.44 6.49 5.56 0.82 25 9.93E−043.54 12 hsa-asg-13254 3.05 4.28 2.57 1.38 0 9.23E−04 6.10 69hsa-asg-13244 9.75 9.41 9.51 0.29 100 1.44E−04 −1.86 4 hsa-asg-132053.59 3.34 3.32 0.74 0 1.42E−04 5.26 38 hsa-asg-13127 5.32 4.78 4.93 0.450 6.06E−04 2.52 6 hsa-asg-12949 8.17 7.88 8.01 0.16 100 4.92E−04 1.37 3hsa-asg-12902 9.54 9.55 9.55 0.07 100 4.82E−04 −1.87 4 hsa-asg-128852.90 2.79 2.89 0.17 0 5.73E−06 2.02 4 hsa-asg-12765 2.90 2.79 2.50 0.590 7.56E−04 2.67 6 hsa-asg-12729 10.85 10.90 11.09 0.27 100 5.32E−05 2.405 hsa-asg-12600 8.35 8.36 8.46 0.12 100 3.57E−04 1.06 2 hsa-asg-124711.84 2.15 2.04 0.23 0 8.80E−05 1.56 3 hsa-asg-12423 8.26 8.24 8.21 0.120 4.50E−04 1.11 2 hsa-asg-12406 8.75 8.50 8.89 0.32 100 2.24E−04 2.71 7hsa-asg-12372 10.32 10.17 10.31 0.10 100 4.45E−06 1.93 4 hsa-asg-121928.15 8.02 8.21 0.20 100 2.54E−04 1.44 3 hsa-asg-11896 11.98 11.92 11.870.15 25 8.27E−04 1.30 2 hsa-asg-11867 3.64 3.25 3.16 0.37 0 8.10E−041.62 3 hsa-asg-11844 2.11 2.92 2.45 0.35 0 5.47E−04 1.74 3 hsa-asg-117643.31 3.82 3.56 0.26 25 1.42E−04 1.61 3 hsa-asg-11572 10.27 10.15 10.500.34 100 7.65E−04 1.76 3 hsa-asg-11527 2.11 2.79 2.54 0.31 0 8.61E−041.67 3 hsa-asg-11515 10.13 10.13 10.14 0.18 100 1.28E−04 2.10 4hsa-asg-11486 11.18 10.64 10.87 0.23 100 4.34E−04 1.65 3 hsa-asg-114432.74 1.94 2.98 0.96 25 7.15E−04 4.94 31 hsa-asg-11428 6.69 5.15 6.000.72 50 7.55E−04 −4.34 20 hsa-asg-114 7.42 6.99 7.38 0.30 0 1.85E−041.72 3 hsa-asg-11373 9.60 9.62 9.44 0.20 50 4.34E−04 1.02 2hsa-asg-11199 9.96 9.76 9.75 0.30 75 3.70E−05 2.52 6 hsa-asg-10981 3.543.34 3.34 0.17 0 1.17E−04 1.39 3 hsa-asg-10883 5.12 4.78 4.36 0.72 03.37E−04 4.21 18 hsa-asg-10577 10.25 10.52 10.59 0.26 100 5.68E−05 2.074 hsa-asg-10473 1.69 2.33 2.33 0.45 0 6.68E−04 2.43 5 hsa-asg-10409 3.193.15 2.95 0.27 0 7.40E−04 1.30 2 hsa-asg-10401 3.73 2.92 3.23 0.35 07.24E−04 1.73 3 hsa-asg-10154 8.42 9.31 9.09 0.59 0 5.46E−04 2.82 7hsa-asg-10113 2.11 2.50 2.01 0.38 0 8.42E−04 2.23 5 hsa-asg-10065 2.112.50 2.34 0.18 0 2.74E−04 1.80 3 hsa-miR257 5.73 4.47 4.23 1.28 752.37E−03 4.57 24 hsa-miR229 2.98 2.15 2.43 0.37 0 2.73E−02 0.79 2hsa-miR223 8.81 8.99 8.77 0.17 100 1.92E−03 −1.00 2 hsa-miR207 8.31 8.517.91 0.59 50 2.06E−02 1.18 2 hsa-miR189 7.59 7.12 7.56 0.40 0 1.66E−020.97 2 hsa-miR166 7.36 7.32 7.21 0.22 25 3.98E−02 0.40 1 hsa-miR157 8.097.17 6.87 0.96 75 2.37E−02 2.21 5 hsa-miR120 2.34 2.15 2.27 0.09 01.83E−02 −0.42 1 hsa-miR102 7.04 7.33 6.69 0.59 100 1.42E−02 1.39 3hsa-asg-999 5.73 6.57 6.23 0.36 100 4.23E−02 1.26 2 hsa-asg-9927 6.165.67 6.39 0.59 100 1.36E−03 −2.51 6 hsa-asg-9886 8.34 8.19 8.36 0.22 1001.96E−02 0.47 1 hsa-asg-9880 11.28 11.33 11.16 0.22 0 1.48E−02 0.53 1hsa-asg-9874 12.54 12.42 12.53 0.09 100 1.25E−02 0.61 2 hsa-asg-98587.52 6.35 7.27 0.83 25 1.80E−02 1.74 3 hsa-asg-9845 4.02 3.82 4.96 1.2850 2.84E−02 −2.63 6 hsa-asg-9735 5.99 5.38 5.95 0.50 100 4.76E−02 0.83 2hsa-asg-9729 6.33 8.52 7.72 0.96 100 4.84E−02 1.48 3 hsa-asg-9714 4.295.33 4.00 1.03 25 2.27E−02 −1.98 4 hsa-asg-9696 2.34 4.12 3.43 1.08 01.06E−02 3.22 9 hsa-asg-9665 12.34 12.14 12.32 0.12 100 2.85E−03 −0.70 2hsa-asg-9649 6.26 4.59 6.21 1.16 75 3.77E−03 −4.25 19 hsa-asg-9625 10.9210.68 10.81 0.31 100 2.44E−02 −0.68 2 hsa-asg-9610 2.90 3.25 3.84 0.9050 2.72E−02 −1.66 3 hsa-asg-9575 3.90 2.79 3.50 0.66 25 4.73E−02 1.58 3hsa-asg-9573 5.27 4.55 5.39 0.66 100 2.95E−02 1.75 3 hsa-asg-9569 1.842.15 1.98 0.31 0 1.04E−02 −0.94 2 hsa-asg-9562 3.05 3.52 2.99 0.50 02.13E−03 2.10 4 hsa-asg-9559 4.83 4.51 4.36 0.47 0 3.25E−03 1.66 3hsa-asg-9547 6.61 6.88 6.68 0.16 100 4.99E−02 −0.37 1 hsa-asg-9530 12.6712.64 12.65 0.01 100 1.82E−04 0.79 2 hsa-asg-9521 2.90 2.15 2.30 0.40 02.67E−02 1.35 3 hsa-asg-9508 11.09 11.07 10.92 0.41 0 3.33E−03 1.37 3hsa-asg-9503 12.56 12.42 12.52 0.09 100 6.76E−03 −0.29 1 hsa-asg-94866.09 5.63 5.60 0.44 50 3.42E−02 −1.56 3 hsa-asg-9476 4.16 5.80 5.35 1.0050 9.15E−03 −2.84 7 hsa-asg-9457 12.37 12.23 12.34 0.08 100 2.70E−04−0.45 1 hsa-asg-9434 8.67 8.43 8.62 0.13 0 1.86E−02 0.40 1 hsa-asg-94112.74 3.60 3.09 0.37 0 8.31E−03 1.35 3 hsa-asg-9368 6.51 7.24 7.17 0.45 03.14E−03 1.69 3 hsa-asg-9332 9.52 9.14 9.46 0.23 100 3.96E−02 0.37 1hsa-asg-9319 7.07 6.66 7.14 0.37 100 1.07E−02 0.98 2 hsa-asg-9297 3.314.12 3.55 0.42 0 3.39E−02 1.74 3 hsa-asg-9273 11.59 11.57 11.63 0.10 1002.03E−02 −0.29 1 hsa-asg-925 3.90 5.76 4.57 1.19 50 2.60E−02 2.22 5hsa-asg-9248 10.19 10.38 10.28 0.09 100 1.27E−02 −0.22 1 hsa-asg-9242.65 3.15 2.17 0.92 0 3.20E−03 4.76 27 hsa-asg-922 6.06 4.95 4.76 1.2825 1.75E−02 2.69 6 hsa-asg-9212 8.91 8.32 8.81 0.34 100 2.34E−03 −6.6399 hsa-asg-916 9.78 10.01 9.94 0.16 100 1.97E−02 0.78 2 hsa-asg-91410.11 10.21 10.09 0.25 100 4.67E−02 0.47 1 hsa-asg-9128 8.19 7.28 7.720.38 100 2.21E−02 0.76 2 hsa-asg-9120 8.29 8.69 8.51 0.20 25 2.57E−020.71 2 hsa-asg-9099 5.26 5.25 5.25 0.25 0 1.14E−02 1.17 2 hsa-asg-909213.01 12.74 12.83 0.21 100 2.49E−02 −0.41 1 hsa-asg-9082 4.29 5.49 4.911.41 0 3.37E−02 2.61 6 hsa-asg-9060 7.22 6.57 7.22 0.52 0 3.60E−02 0.922 hsa-asg-9052 8.32 8.72 8.80 0.37 50 5.71E−03 −1.10 2 hsa-asg-9025 6.406.68 6.91 0.45 100 2.24E−02 0.99 2 hsa-asg-9008 2.82 5.09 4.26 1.06 02.55E−02 −2.70 7 hsa-asg-9004 7.92 8.35 8.40 0.35 100 3.78E−03 1.50 3hsa-asg-8972 3.12 4.78 3.63 1.27 0 1.22E−02 2.91 8 hsa-asg-8970 5.376.53 6.29 0.62 75 1.99E−02 −1.27 2 hsa-asg-8957 9.42 9.37 9.48 0.11 1001.06E−02 0.48 1 hsa-asg-8955 3.64 5.15 4.15 2.04 25 1.77E−02 4.38 21hsa-asg-8953 10.62 10.39 10.48 0.15 100 8.10E−04 0.86 2 hsa-asg-89287.91 8.25 8.12 0.16 0 5.16E−03 0.54 1 hsa-asg-8925 7.86 7.88 8.27 0.64 03.23E−02 −5.81 56 hsa-asg-8889 3.64 2.50 3.65 0.82 0 7.43E−03 2.46 6hsa-asg-8882 5.23 5.76 5.60 0.44 50 4.54E−02 1.39 3 hsa-asg-8878 9.589.38 9.73 0.34 100 1.91E−03 1.23 2 hsa-asg-8872 9.81 9.34 9.80 0.33 1003.64E−03 −1.09 2 hsa-asg-8770 4.32 4.33 4.10 0.39 75 2.17E−02 1.40 3hsa-asg-8757 4.50 5.85 4.95 0.77 0 1.73E−03 −3.01 8 hsa-asg-8699 4.745.95 5.74 0.67 25 1.37E−02 −1.61 3 hsa-asg-8687 8.24 9.08 8.73 0.38 254.38E−02 0.86 2 hsa-asg-8670 3.78 2.33 3.19 0.77 0 3.83E−02 −1.30 2hsa-asg-8642 4.65 3.44 4.18 0.55 0 3.51E−02 1.27 2 hsa-asg-8623 9.639.45 9.60 0.12 100 4.02E−02 0.36 1 hsa-asg-8617 10.26 9.76 10.10 0.36100 8.61E−03 1.15 2 hsa-asg-8607 9.35 9.80 9.61 0.19 75 9.39E−03 0.71 2hsa-asg-8602 7.84 7.75 7.74 0.08 50 1.83E−02 −0.37 1 hsa-asg-8579 3.313.52 3.03 0.48 0 1.12E−02 1.69 3 hsa-asg-8576 11.76 11.57 11.77 0.15 1002.75E−02 0.31 1 hsa-asg-8520 10.52 10.42 10.51 0.09 100 3.12E−02 0.35 1hsa-asg-8518 7.73 7.80 7.76 0.38 100 2.60E−03 1.30 2 hsa-asg-8515 11.3111.16 11.24 0.07 100 9.99E−04 −0.50 1 hsa-asg-8510 10.06 10.10 10.100.13 100 6.21E−03 0.52 1 hsa-asg-8459 8.81 8.53 8.72 0.13 100 3.21E−020.63 2 hsa-asg-8454 4.38 3.82 3.99 0.40 50 1.20E−03 1.79 3 hsa-asg-845011.70 11.32 11.65 0.24 100 1.06E−03 −1.06 2 hsa-asg-8444 6.96 6.90 6.840.38 100 3.39E−03 1.59 3 hsa-asg-8421 10.74 10.91 10.89 0.12 1004.19E−03 0.46 1 hsa-asg-8411 4.98 2.15 3.89 1.34 50 3.83E−02 2.41 5hsa-asg-8391 6.86 6.43 6.82 0.32 100 3.56E−03 1.34 3 hsa-asg-8379 3.825.55 4.67 0.89 0 8.13E−03 2.44 5 hsa-asg-8357 2.74 2.33 2.40 0.40 03.57E−03 1.69 3 hsa-asg-8300 2.74 2.79 3.41 0.82 0 4.19E−02 2.19 5hsa-asg-8297 3.31 3.52 3.35 0.17 0 1.02E−02 1.19 2 hsa-asg-8282 5.944.18 5.58 0.94 75 2.06E−02 −1.87 4 hsa-asg-8280 3.37 6.27 5.64 1.57 752.79E−02 −3.40 11 hsa-asg-8257 6.03 6.75 6.55 0.44 100 1.80E−03 1.97 4hsa-asg-8253 10.97 11.11 11.15 0.15 100 1.17E−03 0.79 2 hsa-asg-82279.82 9.73 9.68 0.12 100 3.81E−02 0.34 1 hsa-asg-8221 9.66 9.37 9.66 0.21100 5.44E−03 1.19 2 hsa-asg-8197 5.21 5.38 5.25 0.12 0 3.62E−03 0.83 2hsa-asg-8163 12.82 12.64 12.80 0.15 100 2.20E−03 −0.59 2 hsa-asg-81375.57 5.15 5.72 0.45 75 9.62E−03 1.75 3 hsa-asg-8130 7.01 6.80 7.30 0.6125 2.25E−02 −5.97 63 hsa-asg-8121 7.40 7.09 7.40 0.27 0 9.53E−03 0.74 2hsa-asg-8099 5.01 5.83 5.17 0.48 25 1.95E−02 −2.34 5 hsa-asg-8084 2.341.94 2.11 0.17 0 2.39E−02 −0.52 1 hsa-asg-8055 2.98 2.15 2.14 0.60 07.15E−03 3.03 8 hsa-asg-8054 2.74 1.46 2.04 0.53 0 4.23E−03 1.82 4hsa-asg-8042 10.85 10.41 10.84 0.30 100 1.36E−03 −1.97 4 hsa-asg-80365.96 2.50 4.44 1.43 25 3.55E−02 2.50 6 hsa-asg-8002 7.60 7.63 7.55 0.77100 6.94E−03 2.33 5 hsa-asg-7973 9.20 8.83 8.96 0.19 100 5.21E−03 1.04 2hsa-asg-7964 7.90 6.92 7.16 0.57 100 1.76E−02 −1.52 3 hsa-asg-7952 4.094.01 3.52 0.83 0 8.89E−03 −2.15 4 hsa-asg-7939 13.93 13.78 13.96 0.15100 1.86E−02 −0.33 1 hsa-asg-7930 8.84 8.95 9.11 0.39 100 1.28E−02 −0.912 hsa-asg-791 2.45 1.71 2.22 0.55 0 7.38E−03 2.07 4 hsa-asg-7900 9.008.33 8.50 0.43 75 5.28E−03 1.63 3 hsa-asg-7859 11.76 11.35 11.56 0.18 03.75E−03 0.59 2 hsa-asg-7777 7.73 7.97 7.83 0.13 0 2.83E−03 1.34 3hsa-asg-7770 6.56 7.23 6.94 0.28 100 1.30E−03 2.38 5 hsa-asg-7753 12.9412.60 12.81 0.15 100 8.66E−03 −0.46 1 hsa-asg-7751 2.65 3.75 3.32 0.50 08.43E−03 2.78 7 hsa-asg-7749 8.43 8.77 8.50 0.34 75 4.75E−02 −0.64 2hsa-asg-773 9.15 8.98 9.31 0.41 50 2.57E−03 1.61 3 hsa-asg-7721 6.436.14 6.65 0.47 0 1.66E−03 1.93 4 hsa-asg-7704 7.48 7.38 7.61 0.22 1001.09E−02 0.83 2 hsa-asg-7690 5.64 4.88 5.32 0.32 100 3.98E−02 −0.65 2hsa-asg-7688 3.05 3.60 2.90 0.56 25 2.99E−03 1.98 4 hsa-asg-7684 2.825.67 5.19 1.92 75 3.87E−02 −3.17 9 hsa-asg-7667 11.65 11.41 11.58 0.11100 1.98E−03 0.49 1 hsa-asg-7651 9.52 9.00 9.47 0.32 100 1.58E−02 0.69 2hsa-asg-7640 9.19 9.07 9.15 0.10 50 5.19E−03 −0.28 1 hsa-asg-7637 5.054.33 4.09 1.31 0 1.65E−02 2.99 8 hsa-asg-7617 9.55 9.59 9.51 0.07 753.19E−03 −0.75 2 hsa-asg-7585 6.40 6.60 6.79 0.35 100 1.13E−02 −1.09 2hsa-asg-7579 8.41 8.20 8.36 0.13 100 1.08E−02 −0.41 1 hsa-asg-7564 5.556.46 6.28 0.49 100 4.70E−02 1.21 2 hsa-asg-7540 7.50 7.02 7.48 0.40 1004.46E−02 −0.90 2 hsa-asg-7532 5.61 5.83 5.37 0.42 25 3.69E−03 1.39 3hsa-asg-7514 11.56 11.41 11.57 0.17 100 3.87E−02 −0.31 1 hsa-asg-751010.56 10.56 10.63 0.09 100 2.94E−02 0.53 1 hsa-asg-7491 8.53 8.20 8.650.46 25 4.45E−02 0.88 2 hsa-asg-7487 6.48 5.76 6.43 0.65 100 4.35E−021.27 2 hsa-asg-7478 3.19 2.79 3.06 0.21 0 5.81E−03 1.28 2 hsa-asg-74711.53 3.04 3.26 1.40 0 4.54E−02 2.40 5 hsa-asg-7465 2.45 1.94 2.73 0.84 03.09E−03 3.90 15 hsa-asg-7463 7.27 7.42 7.26 0.26 25 2.38E−02 0.68 2hsa-asg-7458 2.90 2.65 3.95 1.36 50 3.89E−02 −2.47 6 hsa-asg-744 12.1211.68 11.85 0.21 100 2.03E−02 0.61 2 hsa-asg-7428 10.54 10.07 10.40 0.23100 1.64E−03 0.85 2 hsa-asg-7426 8.78 8.82 8.95 0.25 100 3.29E−03 0.86 2hsa-asg-7424 8.31 8.03 8.04 0.20 0 4.60E−02 −0.67 2 hsa-asg-7423 1.842.92 2.26 0.46 0 1.74E−02 2.48 6 hsa-asg-7410 7.71 7.58 7.85 0.24 1006.31E−03 0.72 2 hsa-asg-7400 11.34 11.51 11.32 0.13 0 1.43E−03 0.81 2hsa-asg-7367 2.11 1.46 1.87 0.30 0 4.24E−02 0.72 2 hsa-asg-7363 0.812.15 1.85 0.86 0 8.72E−03 2.18 5 hsa-asg-7319 2.11 2.50 2.56 0.42 258.46E−03 1.08 2 hsa-asg-7314 5.35 5.76 5.40 0.68 75 2.84E−02 −2.02 4hsa-asg-7302 2.55 2.33 2.05 0.58 0 4.68E−03 1.90 4 hsa-asg-730 10.2610.07 10.32 0.23 100 3.32E−02 −0.43 1 hsa-asg-7292 10.11 9.72 9.83 0.21100 1.40E−02 0.64 2 hsa-asg-7281 3.19 2.15 2.56 0.46 0 3.65E−03 1.66 3hsa-asg-7258 6.75 5.53 6.47 0.63 75 3.16E−02 −2.50 6 hsa-asg-7252 3.984.01 4.05 0.11 0 3.48E−02 0.24 1 hsa-asg-7239 3.54 3.25 3.13 0.33 02.21E−03 1.43 3 hsa-asg-723 3.31 3.04 2.73 0.52 0 1.38E−02 1.23 2hsa-asg-7224 8.19 8.34 8.50 0.29 0 5.64E−03 0.80 2 hsa-asg-7221 9.379.33 9.52 0.20 100 2.00E−03 0.92 2 hsa-asg-7217 10.96 11.37 11.12 0.18 01.54E−02 0.43 1 hsa-asg-7201 7.91 8.07 7.83 0.19 100 1.38E−02 0.48 1hsa-asg-7200 10.21 9.84 9.97 0.17 100 4.59E−02 0.30 1 hsa-asg-7199 9.689.35 9.63 0.23 100 1.37E−02 0.77 2 hsa-asg-7175 13.70 13.49 13.62 0.17100 2.84E−02 −0.39 1 hsa-asg-7173 10.27 9.93 10.10 0.14 100 3.81E−02−0.43 1 hsa-asg-7097 11.61 11.55 11.48 0.12 0 2.05E−02 0.26 1hsa-asg-7096 12.95 12.80 12.92 0.17 100 2.73E−02 −0.38 1 hsa-asg-70709.73 9.99 9.79 0.14 100 3.22E−02 −0.52 1 hsa-asg-7062 8.98 9.04 8.920.11 100 1.27E−02 −0.80 2 hsa-asg-7025 10.79 10.72 10.57 0.30 1001.92E−02 0.65 2 hsa-asg-7009 8.61 8.02 8.42 0.30 100 3.30E−03 1.04 2hsa-asg-6926 4.02 4.33 3.98 0.26 0 1.63E−02 1.22 2 hsa-asg-6922 1.692.50 1.77 0.51 0 3.89E−02 1.26 2 hsa-asg-6910 8.98 9.23 8.61 0.80 1002.82E−02 1.46 3 hsa-asg-6898 2.90 3.04 2.88 0.30 0 8.33E−03 1.32 2hsa-asg-6883 2.98 3.04 2.64 0.45 0 1.33E−02 2.04 4 hsa-asg-6860 9.669.79 9.74 0.10 100 1.30E−04 0.93 2 hsa-asg-6856 3.94 3.52 3.90 0.32 01.67E−02 0.74 2 hsa-asg-6831 10.44 10.16 10.31 0.16 100 9.19E−04 −0.66 2hsa-asg-6823 7.46 5.30 6.65 0.94 75 4.06E−02 1.58 3 hsa-asg-682 6.897.06 6.92 0.13 100 2.78E−02 0.67 2 hsa-asg-6782 6.61 6.56 6.57 0.32 754.53E−02 0.52 1 hsa-asg-6754 6.18 4.98 6.05 0.77 0 4.50E−02 −1.51 3hsa-asg-6733 3.64 3.68 3.58 0.61 25 1.75E−02 1.27 2 hsa-asg-6729 7.326.79 7.54 0.61 100 9.01E−03 1.83 4 hsa-asg-6728 10.65 10.26 10.69 0.41100 4.24E−02 −0.69 2 hsa-asg-6633 4.70 5.18 4.68 0.37 0 3.55E−02 1.10 2hsa-asg-657 7.84 7.60 7.73 0.29 0 7.60E−03 1.32 2 hsa-asg-6569 6.11 6.936.31 0.46 75 1.76E−02 1.13 2 hsa-asg-6532 3.05 4.18 3.34 0.56 254.69E−02 1.06 2 hsa-asg- 12.37 12.04 12.29 0.20 100 1.09E−03 −0.84 26507_st2 hsa-asg-6500 7.83 7.48 7.83 0.27 100 1.67E−02 0.76 2hsa-asg-6466 12.35 12.06 12.10 0.24 75 4.21E−02 −0.38 1 hsa-asg-644 6.076.38 5.87 0.79 75 3.54E−02 1.76 3 hsa-asg-6412 8.43 8.33 8.35 0.50 254.60E−02 0.92 2 hsa-asg-641 2.11 2.65 2.14 0.46 0 1.31E−02 1.46 3hsa-asg-6383 8.01 8.24 8.23 0.16 0 4.36E−03 1.06 2 hsa-asg-6371 10.6210.97 10.84 0.16 100 1.98E−02 0.44 1 hsa-asg-6341 13.64 13.38 13.58 0.15100 1.64E−03 −0.79 2 hsa-asg-6331 7.77 8.22 8.11 0.24 100 2.02E−02 0.531 hsa-asg-6239 1.69 2.15 1.74 0.50 0 2.59E−02 1.15 2 hsa-asg-6203 11.7811.46 11.68 0.21 100 4.58E−03 −0.70 2 hsa-asg-6202 8.02 8.43 8.07 0.2550 2.25E−02 0.56 1 hsa-asg-6186 3.54 4.81 4.13 0.54 0 1.78E−02 1.90 4hsa-asg-6180 11.04 10.70 11.01 0.29 100 6.95E−03 0.76 2 hsa-asg-61683.43 2.33 2.80 0.46 0 2.41E−02 −0.89 2 hsa-asg-6165 3.48 4.18 4.14 0.4725 2.61E−02 −1.66 3 hsa-asg-6149 9.70 9.87 9.76 0.10 75 1.50E−04 0.81 2hsa-asg-6138 6.91 6.01 5.46 1.29 50 3.66E−02 2.31 5 hsa-asg-6116 5.454.33 3.99 1.15 50 2.45E−02 −2.17 5 hsa-asg-6092 12.23 12.01 12.13 0.11100 4.09E−04 −0.66 2 hsa-asg-6071 6.82 6.57 6.72 0.11 100 1.70E−03 −1.312 hsa-asg-6021 9.26 9.20 9.36 0.15 50 2.13E−02 0.39 1 hsa-asg-6019 7.017.80 7.84 0.64 50 4.95E−02 −1.16 2 hsa-asg-6018 10.24 9.98 10.22 0.17100 2.94E−02 −0.75 2 hsa-asg-6000 6.61 7.53 7.20 0.58 0 2.42E−02 1.13 2hsa-asg-5960 9.92 9.78 10.08 0.27 100 2.51E−03 1.16 2 hsa-asg-5952 4.293.44 4.02 0.39 0 2.27E−03 1.89 4 hsa-asg-5889 4.81 6.15 5.18 0.94 256.59E−03 2.49 6 hsa-asg-5873 2.74 3.25 3.08 0.59 0 5.91E−03 1.95 4hsa-asg-585 12.97 12.72 12.77 0.17 100 1.66E−02 −0.45 1 hsa-asg-58275.93 3.75 4.46 1.08 25 6.09E−03 3.01 8 hsa-asg-5810 10.08 10.34 10.150.13 100 4.13E−02 −0.39 1 hsa-asg-5773 6.90 7.36 6.96 0.29 0 1.22E−021.33 3 hsa-asg-5728 9.75 9.44 9.58 0.37 100 2.43E−03 1.48 3 hsa-asg-5726.71 6.56 7.01 0.47 0 1.17E−02 1.85 4 hsa-asg-5695 2.11 2.65 2.55 0.30 02.01E−03 1.74 3 hsa-asg-562 7.97 8.06 7.74 0.59 100 1.01E−02 2.10 4hsa-asg-5519 6.06 7.72 6.94 0.70 25 1.84E−02 −1.76 3 hsa-asg-5511 6.807.21 7.21 0.41 100 3.96E−02 1.17 2 hsa-asg-547 2.11 2.33 2.02 0.34 04.98E−02 0.52 1 hsa-asg-5467 4.55 3.60 4.09 0.40 75 6.04E−03 −1.12 2hsa-asg-5451 4.16 3.75 3.57 0.63 0 5.40E−03 1.86 4 hsa-asg-5421 7.767.24 7.42 0.31 25 8.72E−03 0.86 2 hsa-asg-5399 9.69 9.79 10.04 0.36 1003.07E−02 −1.61 3 hsa-asg-5365 6.18 5.55 5.91 0.43 50 3.76E−02 −1.47 3hsa-asg-5326 8.26 7.65 8.02 0.28 100 3.77E−03 −1.76 3 hsa-asg-5304 7.647.24 7.29 0.25 0 2.35E−03 1.62 3 hsa-asg-5290 3.59 5.53 4.80 0.87 752.46E−02 −1.75 3 hsa-asg-529 3.59 3.34 3.86 0.66 25 8.06E−03 −1.93 4hsa-asg-5289 10.02 9.83 9.74 0.30 75 1.90E−02 −0.64 2 hsa-asg-5240 3.543.52 3.51 0.10 75 5.76E−04 0.62 2 hsa-asg-5227 3.64 4.38 4.35 0.63 04.05E−02 1.27 2 hsa-asg-5176 3.19 1.94 2.86 0.73 0 2.93E−03 2.61 6hsa-asg-5134 2.11 1.71 2.19 0.48 0 1.86E−02 1.61 3 hsa-asg-5122 9.639.65 9.45 0.25 100 8.36E−03 1.31 2 hsa-asg-5115 10.03 10.23 9.99 0.32100 3.37E−03 0.98 2 hsa-asg-5109 10.81 10.93 11.06 0.24 100 8.06E−030.73 2 hsa-asg-509 4.20 4.18 4.23 0.49 25 1.53E−03 −1.90 4 hsa-asg-508510.28 10.13 10.34 0.18 100 1.80E−02 0.54 1 hsa-asg-5061 9.98 9.32 9.730.36 100 4.69E−02 −0.74 2 hsa-asg-5053 4.20 2.92 3.57 0.64 25 4.51E−021.05 2 hsa-asg-5005 8.29 8.42 8.34 0.30 0 2.61E−03 1.06 2 hsa-asg-50004.47 5.18 5.34 0.93 75 3.61E−02 1.56 3 hsa-asg-4990 9.06 9.65 9.20 0.33100 4.31E−03 1.18 2 hsa-asg-4971 9.26 9.28 9.36 0.15 100 5.56E−03 0.62 2hsa-asg-4914 1.00 1.94 1.30 0.44 0 8.23E−03 2.61 6 hsa-asg-4912 10.309.95 10.28 0.23 100 8.42E−04 0.99 2 hsa-asg-4892 3.64 3.75 3.66 0.11 01.53E−02 0.34 1 hsa-asg-4854 8.94 9.16 9.10 0.12 100 6.85E−04 −1.00 2hsa-asg-4847 8.56 8.54 8.96 0.47 100 1.86E−02 0.98 2 hsa-asg-4844 10.109.87 9.90 0.19 100 4.82E−02 −0.57 1 hsa-asg-4840 5.05 3.60 3.91 0.79 251.93E−02 2.01 4 hsa-asg-4839 12.99 12.70 12.81 0.17 100 2.22E−02 −0.40 1hsa-asg-481 2.23 1.46 1.85 0.31 0 1.29E−02 2.19 5 hsa-asg-4722 8.54 8.138.08 0.35 0 2.23E−03 −1.44 3 hsa-asg-471 10.75 10.92 10.79 0.09 1002.93E−04 −0.64 2 hsa-asg-4703 7.02 7.61 7.59 0.46 100 4.24E−02 −1.16 2hsa-asg-47 10.85 10.58 10.65 0.17 100 1.47E−02 0.59 2 hsa-asg-468 7.868.10 8.15 0.28 100 1.02E−03 1.50 3 hsa-asg-4654 12.44 12.17 12.28 0.13100 2.32E−02 −0.26 1 hsa-asg-4618 5.98 7.19 6.88 0.60 100 4.42E−02 −1.092 hsa-asg-459 12.45 12.31 12.37 0.09 100 2.53E−04 −0.60 2 hsa-asg-45806.23 5.30 6.10 0.55 75 4.90E−02 −0.87 2 hsa-asg-458 8.97 8.75 9.33 0.59100 5.17E−03 −1.79 3 hsa-asg-4529 10.42 9.98 10.13 0.28 100 1.10E−020.94 2 hsa-asg-4469 9.28 8.88 8.76 0.43 100 1.30E−02 0.99 2 hsa-asg-44587.55 8.39 7.74 0.56 100 2.04E−02 1.23 2 hsa-asg-4448 3.90 3.15 3.04 0.750 4.02E−02 1.27 2 hsa-asg-430 3.59 5.85 4.74 0.95 75 3.65E−03 −3.00 8hsa-asg-4262 8.00 7.46 7.85 0.42 100 4.83E−02 0.72 2 hsa-asg-425 8.157.02 7.82 0.54 100 4.18E−03 2.15 4 hsa-asg-4236 3.94 2.33 3.26 0.68 03.37E−02 1.33 3 hsa-asg-4223 11.91 11.75 11.85 0.07 100 9.52E−05 0.73 2hsa-asg-4222 7.63 7.83 7.62 0.46 100 2.57E−03 1.81 3 hsa-asg-4209 9.189.09 9.31 0.23 100 1.42E−03 1.12 2 hsa-asg-4199 6.53 6.49 6.93 0.49 1003.90E−02 0.88 2 hsa-asg-4172 3.05 4.28 3.40 0.59 25 1.60E−02 1.41 3hsa-asg-4146 8.92 8.40 8.71 0.22 100 2.18E−02 0.75 2 hsa-asg-4122 2.342.15 2.63 0.61 0 1.42E−02 1.83 4 hsa-asg-412 9.53 9.30 9.48 0.15 1004.00E−02 0.31 1 hsa-asg-4114 3.64 4.33 3.87 0.34 25 1.77E−02 1.27 2hsa-asg-4075 2.55 1.94 2.26 0.29 0 4.75E−02 1.36 3 hsa-asg-4064 4.925.51 5.18 0.68 100 4.77E−03 −3.02 8 hsa-asg-4061 9.33 9.12 9.37 0.19 1002.20E−03 1.03 2 hsa-asg-4059 10.19 10.30 10.16 0.21 100 9.73E−03 −0.57 1hsa-asg-4057 2.11 1.71 1.88 0.17 0 3.36E−02 0.43 1 hsa-asg-4045 5.515.82 5.57 0.41 0 1.34E−03 −1.84 4 hsa-asg-4023 9.41 9.35 9.51 0.15 1002.50E−02 −0.65 2 hsa-asg-4019 6.41 6.75 6.82 0.31 100 2.81E−02 0.57 1hsa-asg-4005 7.42 7.24 7.55 0.33 100 2.87E−02 −0.98 2 hsa-asg-4002 8.347.93 8.01 0.24 100 1.03E−02 0.98 2 hsa-asg-3999 10.46 10.47 10.63 0.20100 7.68E−03 0.63 2 hsa-asg-3988 8.55 8.38 8.62 0.19 100 9.35E−03 0.49 1hsa-asg-3983 10.46 9.76 10.18 0.31 0 3.75E−03 1.42 3 hsa-asg-3977 5.555.07 4.83 0.81 75 2.96E−02 1.46 3 hsa-asg-3970 3.43 4.42 4.20 0.52 754.30E−03 2.20 5 hsa-asg-395 9.59 9.44 9.74 0.28 100 4.20E−02 −0.47 1hsa-asg-3941 8.85 8.84 9.06 0.26 50 3.20E−03 1.36 3 hsa-asg-3915 10.4810.46 10.51 0.06 100 2.44E−03 0.29 1 hsa-asg-3910 2.82 2.65 2.17 0.73 03.13E−03 3.30 10 hsa-asg-3882 4.72 4.88 5.11 0.55 100 2.01E−02 1.10 2hsa-asg-3854 2.74 4.23 2.63 1.30 25 3.46E−02 2.25 5 hsa-asg-3842 2.342.65 2.49 0.21 0 3.62E−02 1.50 3 hsa-asg-3822 1.36 3.68 2.80 1.05 04.69E−02 2.18 5 hsa-asg-3807 2.65 1.94 2.55 0.41 0 1.78E−02 2.32 5hsa-asg-3786 8.93 8.73 8.79 0.22 100 4.14E−02 −0.40 1 hsa-asg-3777 9.219.00 9.08 0.25 25 3.46E−03 −0.94 2 hsa-asg-377 11.63 11.35 11.55 0.14 01.10E−02 0.54 1 hsa-asg-3722 10.02 10.05 10.02 0.19 100 2.50E−03 1.17 2hsa-asg-3715 9.98 9.96 10.17 0.25 100 1.59E−02 −0.63 2 hsa-asg-3714 7.857.83 7.92 0.10 100 4.11E−03 −0.71 2 hsa-asg-3694 8.85 8.83 8.73 0.16 258.99E−03 −0.95 2 hsa-asg-3654 7.11 7.36 7.08 0.46 0 2.10E−03 1.62 3hsa-asg-3637 10.66 10.28 10.68 0.33 100 1.33E−02 0.78 2 hsa-asg-35513.05 3.89 3.35 0.70 0 3.64E−02 1.23 2 hsa-asg-355 2.74 3.75 3.13 0.45 01.28E−02 1.04 2 hsa-asg-3516 4.02 4.12 4.30 0.30 50 3.75E−02 −0.72 2hsa-asg-3513 3.43 3.25 3.32 0.24 0 1.50E−03 1.00 2 hsa-asg-3464 13.7813.52 13.72 0.15 100 2.87E−03 −0.49 1 hsa-asg-3462 12.33 12.09 12.260.14 100 7.82E−04 −0.67 2 hsa-asg-3383 11.14 11.19 11.48 0.37 1001.40E−02 0.86 2 hsa-asg-3360 7.62 7.78 7.60 0.14 100 6.87E−03 0.44 1hsa-asg-3345 11.65 11.51 11.57 0.13 100 9.62E−03 0.53 1 hsa-asg-32964.06 4.71 4.42 0.47 0 2.33E−02 1.06 2 hsa-asg-3291 8.34 8.27 8.43 0.26100 9.63E−03 −0.70 2 hsa-asg-3290 9.05 9.15 9.17 0.14 50 2.72E−03 0.49 1hsa-asg-3278 9.33 8.59 8.65 0.46 25 2.63E−03 1.62 3 hsa-asg-3271 10.9410.78 10.99 0.20 100 2.04E−03 −0.71 2 hsa-asg-3265 5.48 5.12 5.25 1.0175 1.37E−02 2.36 5 hsa-asg-3231 12.48 12.13 12.30 0.14 100 4.27E−03 0.712 hsa-asg-32 8.11 8.14 7.98 0.26 100 2.83E−03 −0.87 2 hsa-asg-3199 5.985.71 5.72 0.18 50 3.65E−03 1.26 2 hsa-asg-3186 11.74 11.55 11.62 0.08 03.35E−02 0.18 1 hsa-asg-3182 7.72 7.42 7.87 0.44 75 1.97E−03 1.74 3hsa-asg-3156 5.99 6.64 6.54 0.39 100 7.54E−03 1.83 4 hsa-asg-3130 12.2111.87 11.98 0.22 100 3.89E−02 −0.41 1 hsa-asg-3124 2.82 3.52 2.46 0.97 01.36E−02 2.43 5 hsa-asg-3112 9.79 9.32 9.51 0.22 100 1.81E−03 −0.97 2hsa-asg-3101 8.51 8.65 8.63 0.08 100 7.02E−03 0.85 2 hsa-asg-3073 8.928.73 9.12 0.35 100 2.70E−02 −0.94 2 hsa-asg-3070 3.43 2.50 2.74 0.58 01.52E−03 2.31 5 hsa-asg-3046 9.10 9.30 9.30 0.14 100 1.02E−02 0.81 2hsa-asg-3038 4.90 5.15 5.03 0.12 0 2.18E−02 0.35 1 hsa-asg-3015 6.383.89 5.06 1.76 50 4.20E−02 −2.99 8 hsa-asg-3013 7.28 6.93 7.12 0.15 06.60E−03 −0.50 1 hsa-asg-2981 4.29 4.71 4.55 0.20 75 8.65E−03 0.51 1hsa-asg-2966 7.57 8.48 7.97 0.38 0 4.21E−02 0.88 2 hsa-asg-2952 3.052.50 2.78 0.36 0 1.17E−02 1.15 2 hsa-asg-2940 3.73 4.38 3.95 0.29 09.42E−03 0.87 2 hsa-asg-2930 2.98 2.92 2.62 0.42 0 1.54E−03 1.96 4hsa-asg-2924 1.36 −0.02 1.48 1.16 0 1.18E−03 4.69 26 hsa-asg-2899 13.2512.89 13.06 0.17 100 3.45E−02 −0.37 1 hsa-asg-2872 11.61 11.90 11.740.16 100 2.05E−02 0.49 1 hsa-asg-2862 9.49 9.63 9.64 0.10 50 2.58E−030.66 2 hsa-asg-2858 9.39 9.36 9.50 0.15 100 4.70E−02 0.38 1 hsa-asg-285610.49 11.27 10.63 0.43 100 3.65E−03 1.38 3 hsa-asg-2855 1.84 2.33 2.320.34 0 2.89E−03 1.45 3 hsa-asg-2843 2.90 2.79 2.70 0.29 0 2.07E−02 0.732 hsa-asg-2660 8.85 8.72 9.15 0.58 100 2.65E−03 −3.95 15 hsa-asg-2659.28 9.28 9.33 0.15 100 3.64E−02 −0.28 1 hsa-asg-2620 3.98 4.38 3.640.68 25 1.50E−02 1.50 3 hsa-asg-2588 9.80 9.81 9.82 0.09 100 9.29E−030.50 1 hsa-asg-2580 11.69 11.54 11.57 0.14 100 6.12E−03 0.55 1hsa-asg-257 1.84 3.15 2.78 0.65 0 4.11E−02 1.28 2 hsa-asg-2565 4.60 3.683.43 0.93 25 2.53E−02 2.19 5 hsa-asg-2563 8.84 8.82 8.86 0.07 1008.80E−04 0.68 2 hsa-asg-2542 7.18 8.58 8.42 1.01 50 3.49E−02 −1.86 4hsa-asg-2541 8.38 8.36 8.57 0.26 25 4.45E−02 0.66 2 hsa-asg-2473 7.226.92 7.30 0.31 100 1.30E−03 −1.41 3 hsa-asg-247 8.41 8.14 8.37 0.15 1001.65E−03 0.60 2 hsa-asg-2459 2.11 2.33 2.35 0.38 0 3.04E−02 1.68 3hsa-asg-2449 6.35 4.42 5.74 1.55 50 1.97E−02 −3.17 9 hsa-asg-2437 7.868.13 8.10 0.17 100 2.56E−02 0.32 1 hsa-asg-2433 3.86 4.01 4.12 0.27 253.02E−02 −0.75 2 hsa-asg-2401 9.27 9.34 9.35 0.11 100 4.41E−02 0.39 1hsa-asg-2371 1.84 2.15 1.91 0.42 0 1.22E−02 1.10 2 hsa-asg-2365 2.230.27 1.71 1.11 0 3.99E−03 3.62 12 hsa-asg-2273 4.70 2.15 2.90 1.20 254.31E−02 1.93 4 hsa-asg-2267 9.59 9.00 9.23 0.40 100 3.87E−02 −0.71 2hsa-asg-2237 7.37 6.41 6.68 0.48 100 1.42E−02 −1.71 3 hsa-asg-2228 10.529.95 10.08 0.30 100 9.02E−03 0.87 2 hsa-asg-2199 11.74 11.89 11.91 0.19100 2.40E−02 −0.35 1 hsa-asg-2195 12.16 11.99 12.13 0.20 100 1.71E−02−0.44 1 hsa-asg-2174 8.24 7.17 7.83 0.47 0 2.48E−02 1.04 2 hsa-asg-21608.46 8.51 8.33 0.24 100 3.31E−02 −0.47 1 hsa-asg-2149 7.47 7.62 7.510.11 100 4.40E−03 −0.35 1 hsa-asg-2138 8.69 8.67 8.78 0.20 25 2.83E−020.66 2 hsa-asg-2094 3.54 3.68 3.19 0.75 0 9.03E−03 2.00 4 hsa-asg-19815.18 4.88 5.33 0.37 0 6.21E−03 2.44 5 hsa-asg-1978 3.82 3.34 3.52 0.42 01.62E−02 0.91 2 hsa-asg-1941 3.90 4.07 3.71 0.33 25 1.72E−02 −0.86 2hsa-asg-1911 9.81 9.90 9.84 0.23 100 7.60E−03 −0.76 2 hsa-asg-1888 10.029.74 10.01 0.27 100 7.95E−03 0.80 2 hsa-asg-1861 10.31 10.35 10.45 0.17100 2.97E−02 −0.41 1 hsa-asg-1858 12.11 11.90 11.93 0.13 50 2.27E−030.84 2 hsa-asg-1852 4.96 4.91 4.89 0.09 0 1.03E−02 −0.80 2 hsa-asg-18385.45 5.12 5.20 0.18 0 4.29E−02 1.14 2 hsa-asg-1827 3.05 2.15 3.05 0.6750 2.15E−02 −1.31 2 hsa-asg-1813 11.63 11.27 11.51 0.17 100 1.94E−02−0.46 1 hsa-asg-1809 10.68 10.70 10.74 0.11 100 2.29E−02 0.25 1hsa-asg-18 8.75 8.16 8.41 0.31 0 1.89E−02 −0.81 2 hsa-asg-1797 9.76 9.749.80 0.08 100 7.02E−03 −0.50 1 hsa-asg-1775 9.96 9.55 9.91 0.30 1003.73E−02 −0.58 1 hsa-asg-1766 2.90 2.50 2.73 0.75 0 2.42E−02 1.47 3hsa-asg-1755 3.05 1.71 2.27 0.71 0 5.45E−03 2.53 6 hsa-asg-1747 2.983.25 4.01 1.04 0 7.49E−03 2.68 6 hsa-asg-1736 9.68 9.24 9.60 0.40 1003.77E−03 1.28 2 hsa-asg-1727 7.65 8.13 7.99 0.23 0 6.40E−03 0.76 2hsa-asg-1726 11.03 11.16 11.05 0.10 100 1.77E−03 −0.64 2 hsa-asg-17169.35 8.85 9.12 0.22 50 1.28E−03 −0.95 2 hsa-asg-1699 10.03 10.05 9.950.11 75 3.46E−03 0.60 2 hsa-asg-1686 7.59 7.99 7.80 0.22 0 1.69E−02 0.511 hsa-asg-1641 8.80 8.74 8.85 0.11 25 1.84E−02 0.94 2 hsa-asg-1591 9.337.80 8.68 0.64 100 4.00E−02 −1.07 2 hsa-asg-1588 5.44 4.91 5.14 0.70 254.09E−02 1.18 2 hsa-asg-1573 11.35 11.27 11.31 0.05 100 3.47E−04 −0.27 1hsa-asg-156 6.21 5.88 6.23 0.30 100 4.05E−02 0.72 2 hsa-asg-1544 2.743.89 2.79 0.83 0 1.17E−02 1.95 4 hsa-asg-1534 2.34 2.15 2.05 0.25 03.89E−03 1.65 3 hsa-asg-1532 7.82 8.25 8.10 0.41 75 4.46E−03 −1.79 3hsa-asg-1519 2.74 4.33 3.65 1.70 50 3.55E−02 2.89 7 hsa-asg-1482 4.133.25 3.79 0.38 75 3.93E−02 1.31 2 hsa-asg-145 9.75 9.97 9.99 0.19 1005.25E−03 1.14 2 hsa-asg-1448 7.79 7.17 7.68 0.42 0 1.80E−03 1.84 4hsa-asg-14242 6.06 6.61 6.64 0.66 100 3.61E−02 −1.50 3 hsa-asg-142378.50 8.98 8.86 0.30 100 1.30E−02 0.81 2 hsa-asg-14233 8.25 7.72 7.660.50 100 2.32E−03 1.91 4 hsa-asg-14217 4.83 6.96 6.21 0.94 0 2.87E−033.11 9 hsa-asg-1420 9.64 9.57 9.34 0.32 100 4.19E−03 1.13 2hsa-asg-14198 2.34 2.92 2.30 0.46 0 1.01E−02 1.79 3 hsa-asg-14197 13.5513.49 13.43 0.14 100 6.78E−03 −0.44 1 hsa-asg-14189 14.67 14.49 14.550.11 100 1.03E−02 0.47 1 hsa-asg-14176 6.94 7.08 7.17 0.71 100 4.03E−021.16 2 hsa-asg-14136 8.55 8.48 8.55 0.08 100 4.14E−02 −0.15 1hsa-asg-14135 3.19 3.68 3.51 0.29 0 3.29E−02 1.40 3 hsa-asg-14133 5.166.08 5.51 0.75 100 2.20E−02 1.54 3 hsa-asg-14068 13.77 13.39 13.56 0.20100 2.28E−02 −0.40 1 hsa-asg-14066 9.29 9.20 9.27 0.11 50 5.23E−04 −0.571 hsa-asg-14053 6.23 5.96 6.09 0.11 0 2.46E−02 −1.56 3 hsa-asg-1403413.36 13.11 13.15 0.15 100 4.45E−02 0.28 1 hsa-asg-14026 2.55 3.75 3.110.50 0 3.95E−02 −1.05 2 hsa-asg-14002 8.73 8.06 8.77 0.51 100 5.66E−031.61 3 hsa-asg-14 3.73 2.92 3.32 0.74 25 1.12E−02 1.79 3 hsa-asg-139884.50 5.40 5.45 1.04 100 6.89E−03 −2.78 7 hsa-asg-13970 7.37 8.13 7.720.37 25 3.23E−02 0.78 2 hsa-asg-13966 9.38 8.14 9.11 0.66 100 1.05E−021.75 3 hsa-asg-13933 8.55 8.63 8.48 0.33 100 2.98E−03 1.44 3hsa-asg-13931 8.25 8.11 8.15 0.42 100 4.57E−02 −1.60 3 hsa-asg-138913.65 13.42 13.59 0.12 100 1.79E−03 −0.53 1 hsa-asg-13882 2.11 2.15 2.050.13 0 1.79E−02 −0.99 2 hsa-asg-13870 9.78 9.36 9.74 0.26 100 3.80E−020.65 2 hsa-asg-13851 5.78 4.74 5.37 0.45 25 4.01E−03 −3.01 8hsa-asg-13819 2.34 2.65 2.24 0.34 0 3.36E−02 0.65 2 hsa-asg-13764 9.509.58 9.73 0.31 100 1.49E−02 −1.16 2 hsa-asg-13736 8.62 8.60 8.72 0.13 251.20E−02 0.66 2 hsa-asg-13730 3.12 3.04 3.92 1.32 25 2.99E−02 −2.38 5hsa-asg-13659 9.34 9.36 9.40 0.18 75 5.67E−03 0.56 1 hsa-asg-13646 6.576.55 6.29 0.54 75 1.22E−03 2.55 6 hsa-asg-13620 13.21 13.07 13.09 0.16100 1.75E−02 −0.40 1 hsa-asg-13611 5.99 6.48 6.37 0.42 100 2.41E−02−2.00 4 hsa-asg-13606 11.89 11.80 11.90 0.19 100 1.60E−02 −0.85 2hsa-asg-13501 6.40 6.08 6.62 0.57 100 1.50E−02 −1.98 4 hsa-asg-135009.70 9.78 9.88 0.24 75 1.21E−02 −0.64 2 hsa-asg-13498 8.77 8.49 8.650.15 100 1.99E−03 −1.47 3 hsa-asg-13450 4.77 4.95 5.64 1.04 25 3.20E−02−2.26 5 hsa-asg-13444 4.70 2.33 3.92 1.14 50 3.43E−02 −1.98 4hsa-asg-13436 6.62 5.57 5.99 0.71 100 1.54E−02 1.79 3 hsa-asg-13404 0.611.71 1.10 0.78 0 4.02E−02 3.18 9 hsa-asg-13387 9.11 9.15 9.30 0.20 754.69E−02 −0.86 2 hsa-asg-1337 6.94 7.38 6.94 0.39 100 2.12E−03 1.62 3hsa-asg-13353 8.24 8.92 8.65 0.54 100 2.52E−03 −4.16 18 hsa-asg-133492.74 3.04 2.87 0.18 0 2.45E−02 1.17 2 hsa-asg-13334 3.31 5.33 4.14 0.870 4.73E−02 1.42 3 hsa-asg-13320 3.86 4.55 4.10 0.41 25 1.46E−02 1.18 2hsa-asg-13306 12.12 12.49 12.39 0.20 100 1.09E−02 0.54 1 hsa-asg-133044.52 4.95 3.87 1.14 0 5.22E−03 3.40 11 hsa-asg-13303 5.85 6.71 6.05 1.05100 4.86E−03 −3.58 12 hsa-asg-13294 4.20 4.47 4.42 0.15 0 2.90E−03 1.152 hsa-asg-13279 2.65 3.75 4.44 1.59 25 7.86E−03 4.09 17 hsa-asg-132702.65 −0.28 1.77 1.47 0 2.67E−02 3.71 13 hsa-asg-13260 10.10 10.32 10.160.16 100 1.99E−02 0.68 2 hsa-asg-1324 1.53 2.79 2.55 0.70 0 6.33E−031.92 4 hsa-asg-13237 8.56 9.37 9.06 0.35 100 5.66E−03 1.00 2hsa-asg-13230 4.06 6.49 4.65 1.48 50 4.73E−02 2.55 6 hsa-asg-13203 9.127.01 8.35 1.00 100 1.22E−03 −4.19 18 hsa-asg-13166 2.55 3.52 2.67 0.6125 3.03E−02 2.40 5 hsa-asg-13143 14.62 14.37 14.42 0.15 100 1.87E−020.47 1 hsa-asg-13097 2.65 4.01 3.53 1.65 25 2.41E−03 5.62 49hsa-asg-13091 9.34 9.37 9.61 0.31 100 8.59E−03 −0.84 2 hsa-asg-1307911.86 11.80 11.77 0.08 0 1.36E−02 −0.32 1 hsa-asg-1306 7.37 8.13 7.890.40 0 2.98E−02 −1.12 2 hsa-asg-13051 3.43 2.92 2.90 0.48 0 4.33E−021.11 2 hsa-asg-12996 8.98 8.59 8.71 0.18 100 1.01E−02 0.59 2hsa-asg-12973 4.38 5.15 4.73 0.71 75 4.29E−02 1.42 3 hsa-asg-12946 12.1011.87 11.96 0.19 100 3.45E−03 −0.65 2 hsa-asg-12943 3.05 3.44 3.24 0.320 3.08E−03 1.21 2 hsa-asg-12941 8.98 8.63 8.96 0.36 100 1.93E−02 −0.78 2hsa-asg-12937 11.39 11.14 11.29 0.12 100 8.03E−03 0.40 1 hsa-asg-129302.74 4.55 3.43 1.35 50 4.51E−02 −2.17 5 hsa-asg-12919 13.30 13.02 13.200.16 100 3.97E−03 −0.55 1 hsa-asg-12897 7.44 6.92 7.18 0.26 0 1.43E−020.67 2 hsa-asg-12880 10.14 9.98 10.22 0.30 100 1.29E−02 −0.73 2hsa-asg-12842 6.11 5.07 6.02 0.78 100 3.77E−02 −2.38 5 hsa-asg-128214.02 4.42 4.28 0.18 0 4.33E−03 1.42 3 hsa-asg-12807 3.05 3.52 3.28 1.190 3.67E−02 2.03 4 hsa-asg-12799 7.05 6.96 7.22 0.70 0 2.43E−02 1.48 3hsa-asg-12775 12.08 11.63 11.93 0.24 100 3.49E−03 −0.75 2 hsa-asg-127464.26 4.01 3.79 0.69 75 3.81E−02 1.21 2 hsa-asg-12734 7.51 6.51 7.13 0.51100 1.71E−02 1.28 2 hsa-asg-12653 6.59 5.18 5.66 0.72 25 1.28E−02 1.67 3hsa-asg-12605 7.84 7.16 7.67 0.54 50 9.02E−03 1.51 3 hsa-asg-12602 1.531.94 1.66 0.19 0 2.23E−03 0.67 2 hsa-asg-12595 9.50 8.96 9.36 0.27 1001.02E−02 −0.99 2 hsa-asg-12581 10.71 11.12 11.09 0.26 100 1.94E−02 −0.541 hsa-asg-12503 6.51 4.85 5.25 0.86 25 4.01E−02 −2.16 4 hsa-asg-124853.05 3.95 3.34 0.41 0 3.79E−02 1.09 2 hsa-asg-12464 2.11 2.15 2.09 0.190 2.58E−02 0.36 1 hsa-asg-12443 3.05 2.65 2.71 0.25 25 5.81E−03 0.94 2hsa-asg-12441 10.86 10.80 10.89 0.13 100 2.15E−03 −0.80 2 hsa-asg-12437.58 7.71 7.70 0.10 25 2.07E−03 −0.96 2 hsa-asg-12424 9.92 9.46 9.500.30 75 1.00E−02 0.92 2 hsa-asg-12421 9.58 9.73 9.86 0.26 100 4.76E−02−0.95 2 hsa-asg-12403 4.74 4.47 4.53 0.17 100 1.60E−02 0.62 2hsa-asg-12386 8.22 8.24 8.41 0.21 0 2.61E−02 −0.91 2 hsa-asg-12331 7.507.32 7.61 0.26 100 3.89E−02 −1.52 3 hsa-asg-12330 3.90 4.38 4.22 0.25 03.95E−02 0.98 2 hsa-asg-1233 11.48 11.17 11.44 0.22 0 4.43E−02 −0.44 1hsa-asg-12309 10.02 10.47 10.11 0.24 100 5.07E−03 0.70 2 hsa-asg-123027.61 8.10 8.17 0.54 0 1.52E−02 1.25 2 hsa-asg-12297 9.75 10.00 9.58 0.40100 2.88E−03 1.37 3 hsa-asg-12289 12.12 12.08 12.18 0.16 100 2.63E−02−0.34 1 hsa-asg-12278 9.68 9.53 9.73 0.16 100 1.25E−02 0.68 2hsa-asg-1227 6.61 6.73 6.53 0.22 100 6.72E−03 −1.18 2 hsa-asg-1224712.22 12.05 12.14 0.07 75 2.33E−02 0.19 1 hsa-asg-12244 8.18 7.60 7.760.28 0 8.40E−03 1.15 2 hsa-asg-12119 5.75 4.23 3.80 1.57 50 3.77E−022.66 6 hsa-asg-12096 2.74 2.50 2.80 0.39 0 2.44E−02 0.78 2 hsa-asg-120586.94 7.80 7.42 0.47 100 2.15E−02 −1.51 3 hsa-asg-12049 9.51 9.65 9.530.08 100 1.32E−05 0.96 2 hsa-asg-12027 2.45 1.94 2.71 0.70 0 6.77E−031.94 4 hsa-asg-1201 10.33 9.96 10.25 0.22 100 1.73E−02 −0.52 1hsa-asg-11997 3.54 4.38 4.25 0.63 0 4.46E−02 1.20 2 hsa-asg-11982 8.718.25 8.41 0.38 0 2.22E−02 1.18 2 hsa-asg-11975 10.43 10.39 10.42 0.15100 5.08E−03 −0.45 1 hsa-asg-11962 8.54 8.65 8.58 0.05 100 2.77E−02 0.421 hsa-asg-11956 8.38 8.20 8.43 0.21 0 3.25E−03 0.79 2 hsa-asg-11946 9.639.66 9.81 0.23 100 8.88E−03 0.77 2 hsa-asg-11940 8.34 8.52 8.51 0.11 1002.12E−03 0.50 1 hsa-asg-11912 5.97 6.72 5.04 1.54 50 2.84E−02 2.82 7hsa-asg-1189 8.28 8.12 8.26 0.60 75 1.18E−02 1.43 3 hsa-asg-11877 6.476.82 6.78 0.24 0 3.25E−02 0.74 2 hsa-asg-11872 3.05 2.92 2.62 0.55 04.03E−02 2.10 4 hsa-asg-11827 5.48 4.12 4.65 0.84 50 2.47E−02 −1.91 4hsa-asg-11818 11.00 11.04 11.13 0.15 100 1.06E−03 0.86 2 hsa-asg-117663.73 3.82 3.80 0.05 25 1.73E−02 −0.32 1 hsa-asg-11758 13.23 12.94 13.080.12 100 2.10E−02 −0.34 1 hsa-asg-11698 11.94 11.53 11.76 0.24 1007.08E−03 −0.70 2 hsa-asg-11686 2.34 1.71 2.06 0.32 0 2.02E−02 −0.64 2hsa-asg-11643 3.19 3.15 2.98 0.23 0 2.23E−03 1.09 2 hsa-asg-11639 3.313.75 3.31 0.50 0 4.96E−02 0.94 2 hsa-asg-11635 4.35 5.01 4.71 1.08 504.21E−02 −2.28 5 hsa-asg-11617 10.30 10.49 10.36 0.24 100 2.65E−02 −0.531 hsa-asg-11612 7.93 7.89 7.91 0.07 0 6.43E−03 1.38 3 hsa-asg-1160810.21 9.63 9.95 0.34 100 3.23E−03 1.09 2 hsa-asg-11606 11.52 11.13 11.300.18 100 8.51E−03 0.57 1 hsa-asg-11579 3.82 3.25 3.12 0.56 0 2.02E−032.18 5 hsa-asg-1157 6.70 6.88 7.07 0.37 0 1.72E−03 1.90 4 hsa-asg-115639.59 9.69 9.59 0.11 100 1.14E−02 1.04 2 hsa-asg-11535 13.66 13.47 13.550.12 100 8.80E−03 0.37 1 hsa-asg-11513 9.48 9.26 9.40 0.15 75 5.98E−040.70 2 hsa-asg-11503 8.00 8.38 8.37 0.30 0 4.54E−02 0.71 2 hsa-asg-11406.96 7.44 7.39 0.30 100 1.63E−02 0.78 2 hsa-asg-11389 12.32 12.14 12.290.15 100 2.94E−03 −0.48 1 hsa-asg-11388 6.18 6.64 6.76 0.46 100 1.67E−020.97 2 hsa-asg-11384 2.90 2.50 2.65 0.46 25 4.61E−02 −0.76 2hsa-asg-11381 8.49 8.30 8.32 0.29 100 2.22E−02 0.87 2 hsa-asg-1133610.88 10.69 10.83 0.09 100 2.82E−02 −0.34 1 hsa-asg-11326 7.46 7.65 7.340.26 100 2.69E−02 0.76 2 hsa-asg-11246 2.34 2.50 2.35 0.35 0 2.35E−02−0.71 2 hsa-asg-11228 9.39 9.59 9.56 0.12 100 7.94E−03 0.49 1hsa-asg-11196 2.74 3.34 3.15 0.49 0 2.82E−02 −0.96 2 hsa-asg-11193 11.9711.98 12.12 0.17 75 7.13E−03 −0.52 1 hsa-asg-11191 3.31 2.92 3.09 0.22 03.63E−03 0.96 2 hsa-asg-11174 8.95 8.79 8.94 0.27 25 1.85E−02 −0.94 2hsa-asg-11130 3.43 4.78 4.10 0.66 50 3.61E−03 −2.14 4 hsa-asg-1111211.41 11.39 11.41 0.13 100 2.60E−02 −0.56 1 hsa-asg-11089 10.76 10.2910.39 0.27 100 3.11E−02 −0.79 2 hsa-asg-11061 4.29 4.42 4.74 1.32 253.26E−02 −2.33 5 hsa-asg-11056 5.59 5.35 5.52 0.42 0 1.43E−02 0.97 2hsa-asg-11002 3.37 3.68 3.22 0.58 0 8.23E−03 2.37 5 hsa-asg-10971 9.478.99 9.19 0.33 50 4.55E−02 0.68 2 hsa-asg-10957 2.82 5.74 4.46 1.22 251.08E−02 −3.48 11 hsa-asg-10932 2.74 2.92 3.31 0.65 0 9.79E−03 1.85 4hsa-asg-10926 5.18 7.15 5.32 1.75 75 2.06E−02 −3.47 11 hsa-asg-109227.47 7.33 7.68 0.35 100 2.68E−02 −1.31 2 hsa-asg-10918 6.57 4.55 5.071.00 50 2.72E−02 2.04 4 hsa-asg-10914 2.74 2.92 2.56 0.36 0 8.84E−031.31 2 hsa-asg-10901 7.15 6.89 7.04 0.18 100 3.16E−02 −0.68 2hsa-asg-10893 3.19 3.15 2.91 0.34 0 4.56E−03 1.52 3 hsa-asg-10862 3.052.50 2.97 0.35 0 1.25E−03 1.53 3 hsa-asg-10860 2.90 1.94 2.24 0.48 03.34E−02 1.19 2 hsa-asg-10810 3.82 3.34 3.49 0.22 0 1.24E−03 1.01 2hsa-asg-10807 8.49 8.56 8.90 0.43 50 4.16E−02 −0.83 2 hsa-asg-10647 5.406.46 5.89 0.66 50 7.36E−03 −2.89 7 hsa-asg-10639 11.76 11.59 11.76 0.12100 6.88E−03 0.56 1 hsa-asg-10638 12.30 12.26 12.23 0.14 100 2.35E−02−0.28 1 hsa-asg-10580 12.78 12.14 12.49 0.29 100 3.04E−03 −1.54 3hsa-asg-10576 9.15 9.32 9.37 0.18 100 4.96E−03 1.43 3 hsa-asg-10556 5.056.60 5.37 0.87 25 2.15E−02 −1.72 3 hsa-as -10512 10.57 10.13 10.41 0.21100 1.97E−02 −0.43 1 hsa-asg-10510 6.24 6.88 6.82 0.60 100 2.95E−02−1.37 3 hsa-asg-10481 6.14 4.18 5.51 0.99 50 4.42E−02 1.66 3hsa-asg-10480 3.98 4.55 4.69 1.20 25 4.19E−02 −2.17 4 hsa-asg-10411 9.719.16 9.86 0.56 100 1.72E−03 −3.35 10 hsa-asg-1038 5.99 6.76 6.84 0.86100 2.24E−03 −2.99 8 hsa-asg-10371 1.53 0.89 1.32 0.43 0 3.85E−03 1.57 3hsa-asg-10273 4.29 6.01 5.70 0.96 75 1.11E−02 −2.83 7 hsa-asg-10263 3.642.33 2.64 0.67 0 5.97E−03 1.92 4 hsa-asg-1026 5.74 5.85 5.66 0.17 1002.58E−02 −0.45 1 hsa-asg-10256 3.82 3.04 3.76 0.78 25 3.64E−02 −1.66 3hsa-asg-10247 2.11 1.94 2.15 0.22 0 1.28E−02 1.42 3 hsa-asg-10235 5.485.25 5.78 0.49 100 1.02E−02 −1.46 3 hsa-asg-10231 1.19 3.34 2.62 1.21 02.15E−02 2.41 5 hsa-asg-10229 3.90 3.15 3.33 0.57 0 1.68E−02 1.47 3hsa-asg-10222 4.50 4.18 4.00 0.42 50 2.08E−02 0.92 2 hsa-asg-10199 9.379.34 9.51 0.20 75 3.44E−03 0.77 2 hsa-asg-10197 9.58 9.55 9.52 0.22 751.60E−02 0.68 2 hsa-asg-10195 8.54 7.80 8.36 0.51 100 5.46E−03 1.47 3hsa-asg-10181 9.50 9.55 9.72 0.24 100 2.65E−03 −1.18 2 hsa-asg-1018 6.656.67 6.60 0.30 100 1.95E−02 1.17 2 hsa-asg-10170 5.09 4.91 4.46 0.67 04.69E−02 1.46 3 hsa-asg-10158 3.86 4.28 4.09 0.28 0 9.61E−03 2.27 5hsa-asg-10141 2.55 3.44 3.13 0.73 25 1.68E−02 −1.95 4 hsa-asg-10081 2.902.50 2.26 0.58 0 2.42E−02 1.63 3 hsa-asg-10076 9.78 9.54 9.65 0.18 1001.30E−02 0.59 2 hsa-asg-10037 6.34 5.15 5.93 0.80 100 3.17E−02 −1.41 3hsa-asg-1001 8.78 9.48 9.23 0.31 100 1.05E−03 1.38 3 hsa-miR-140 4.795.51 4.85 1.83 25 2.11E−02 3.71 13 hsa-miR-29b 8.96 9.82 9.14 0.69 1001.93E−03 2.55 6 hsa-miR-329 2.55 1.94 1.98 0.40 0 3.19E−02 3.83 14hsa-miR-329 2.55 3.60 2.97 0.78 0 6.61E−03 3.59 12 hsa-miR-365 2.55 2.332.05 0.58 0 4.53E−04 5.11 34 hsa-miR-369- 2.65 2.65 2.29 0.63 0 1.63E−033.79 14 5p hsa-miR-376a 1.84 3.34 2.90 0.80 0 7.07E−04 4.59 24hsa-miR-376a 2.11 1.46 1.75 0.28 0 1.02E−03 4.20 18 hsa-miR-421 1.534.07 2.67 1.50 0 3.41E−03 4.79 28 hsa-miR-451 8.72 7.76 8.78 0.73 1006.76E−05 5.32 40 hsa-miR-497 8.38 9.13 8.49 0.45 0 4.09E−05 4.08 17hsa-miR-517a 1.98 1.46 1.90 0.35 0 5.22E−05 5.00 32 hsa-miR-551b 2.903.68 2.73 1.29 0 6.93E−04 5.88 59 hsa-miR-99b 8.73 8.43 8.45 0.24 06.16E−06 3.03 8 hsa-miR- 10.54 10.96 10.44 0.50 100 8.60E−05 3.63 12199a* hsa-miR-224 5.53 5.98 5.30 0.58 100 1.37E−05 6.62 98 SD, standarddeviation; %, percentage of samples expressing miRNA above backgroundlevels; Log2Diff, difference in VSN-transformed expression between NBrand LNneg samples.

Example 42 Common miRNAs Differentially Expressed between Normal BreastTissue Samples and Cancer-negative Axillary Lymph Nodes and betweenCancer-positive and Cancer-negative Axillary Lymph Nodes from BreastCancer Patients

The inventors determined the common miRNAs that are differentiallyexpressed between normal breast tissue (NBr) samples and cancer-negativeaxillary lymph nodes (LNneg) (identified in Example 41) and betweencancer-positive (LNpos) and cancer-negative (LNneg) axillary lymph nodesfrom breast cancer patients (identified in Example 40). For thisanalysis, differentially expressed miRNAs were identified, using ap-value<0.05 and a fold-change>2 (Table 39 and Table 40). These miRNAsrepresent particularly useful biomarkers for monitoring the presence ofbreast cancer metastasis to axillary lymph nodes.

Among the 50 verified miRNAs expressed at higher levels in LNpos than inLNneg samples, 45 also had average levels of expression higher in NBrtissues than in LNneg samples (Table 41). Among these, five miRNAs(hsa-miR-200b, -200a, -141, -200c, and -182) had a level of expressionbetween 50- and 500-fold lower in LNneg than their average level ofexpression in either NBr or LNpos samples.

TABLE 41 miRNAs differentially expressed between both normal breast(NBr) tissue samples and cancer-negative axillary lymph nodes (LNneg)and between cancer-positive (LNpos) and cancer-negative (LNneg) axillarylymph nodes. Fold Fold Change Change (NBr vs (LNpos vs Probe ID MicroRNALNneg) LNneg) hsa-miR-375_st1 hsa-miR-375 24 325 hsa-miR-200b_st1hsa-miR-200b 499 322 hsa-miR-141_st1 hsa-miR-141 162 132hsa-miR-200a_st2 hsa-miR-200a 174 107 hsa-miR-182_st1 hsa-miR-182 55 84hsa-miR-200c_st1 hsa-miR-200c 65 56 hsa-miR-203_st2 hsa-miR-203 19 32hsa-miR-429_st1 hsa-miR-429 39 17 hsa-miR-542-5p_st2 hsa-miR-542-5p 1612 hsa-miR-421-3p_st1 hsa-miR-421 35 12 hsa-miR-181d_st1 hsa-miR-181d 89 hsa-miR-213_st1 hsa-miR-181a* 12 9 hsa-miR-489_st2 hsa-miR-489 21 9hsa-miR-224_st2 hsa-miR-224 86 8 hsa-miR-34b_st2 hsa-miR-34b 6 8hsa-miR-21_st2 hsa-miR-21 10 7 hsa-miR-218_st2 hsa-miR-218 68 6hsa-miR-30a-3p_st1 hsa-miR-30a-3p 27 5 hsa-asg-14166_st2 hsa-miR-425-5p4 5 hsa-miR-189_st1 hsa-miR-189 36 4 hsa-miR-148a_st1 hsa-miR-148a 15 4hsa-miR-148b_st2 hsa-miR-148b 22 4 hsa-miR-193a_st1 hsa-miR-193a 11 4hsa-miR-106b_st2 hsa-miR-106b 12 4 hsa-miR-376a_st2 hsa-miR-376a 17 4hsa-miR-452_st1 hsa-miR-452 48 3 hsa-miR-98_st1 hsa-miR-98 18 3hsa-miR-34a_st1 hsa-miR-34a 5 3 hsa-miR-27a_st2 hsa-miR-27a 17 3hsa-miR-128a_st2 hsa-miR-128a 10 3 hsa-miR-93_st2 hsa-miR-93 9 3hsa-miR-22_st1 hsa-miR-22 22 3 hsa-miR-151_st2 hsa-miR-151 9 2hsa-miR-30e-5p_st2 hsa-miR-30e-5p 12 2 hsa-miR-30a-5p_st2 hsa-miR-30a-5p12 2 hsa-miR-99b_st1 hsa-miR-99b 8 2 hsa-miR-191_st2 hsa-miR-191 3 2hsa-miR-107_st2 hsa-miR-107 6 2 hsa-miR-130b_st1 hsa-miR-130b 3 2hsa-miR-181a_st1 hsa-miR-181a 3 2 hsa-miR-24_st1 hsa-miR-24 8 2hsa-miR-199a_st2 hsa-miR-199a 9 2 hsa-miR-103_st1 hsa-miR-103 5 2hsa-miR-25_st2 hsa-miR-25 6 2 hsa-miR-363_st2 hsa-miR-363 5 2

Example 43 qRT-PCR Verification of Differentially Expressed miRNAs inNormal Breast Tissue and Cancer-positive and Cancer-negative Lymph Nodesfrom Breast Cancer Patients

The inventors also evaluated the differential expression of selectedmiRNAs (identified in Example 40 and Example 41) in LNpos and LNnegsamples from breast cancer patients (Table 38, Example 40), by qRT-PCR.qRT-PCR reactions were performed using Taqman® MicroRNA Assays (AppliedBiosystems; Foster City, Calif., USA) specific for eleven miRNAsdifferentially expressed between LNpos and LNneg and between NBr andLNneg samples (hsa-miR-200b, -200a, -203, -182, -200c, -429, -141, -375,-196a, -205, and -183). Assays were performed according to themanufacturer's instructions with 15 ng of input total RNA from the sevensamples (LNpos2-4 and LNneg1-4). Reactions were incubated in a 7900HTReal-Time PCR system (Applied Biosystems, Foster City, Calif., USA).Initial data analysis was done using the 7500 Fast System SDS V2.3software (Applied Biosystems). miR-638, whose expression level did notchange between the different samples, was used for normalization.Results of qRT-PCR expression analysis for the eleven miRNAs werecompared with those obtained from microarray expression analysis (FIG.13A, FIG. 13B) and confirmed the microarray results. Interestingly, theaverage expression level of hsa-miR-205 was higher in only two (LNpos2and 3) of the three positive lymph node samples compared to the LNnegsamples. In one positive lymph node (LNpos4), however, the averageexpression level of miR-205 was reduced compared to that observed innegative lymph nodes, indicating some heterogeneity in the expression ofmiR-205 in metastatic axillary lymph nodes of breast cancer patients.

Example 44 Microarray Analysis of miRNAs Differentially Expressedbetween a Distinct Group of Cancer-positive and Cancer-negative LymphNodes from Breast Cancer Patients

A second and independent set of FFPE samples often cancer-positive(LNpos5-14) and five cancer-negative (LNneg5-9) axillary lymph nodesfrom ductal breast cancer patients were purchased from ProteoGenex(ProteoGenex, Inc.; Culver City, Calif., USA) (Table 42). A pathologyreport and hematoxilin-eosin (H&E)-stained slides were available foreach lymph node sample. H&E slides were reviewed by a pathologist atAsuragen to confirm lymph node statuses as cancer cell-positive orcancer cell-negative.

TABLE 42 Patient and tissue sample pathology information for tencancer-positive (LNpos) and five cancer-negative (LNneg) axillary lymphnodes from 15 independent ductal breast cancer patients. TNM stage(Sobin and Wittekind, 2002); AJCC Stage (Greene, 2002); LN Mets, numberof lymph nodes with metastases/total number of lymph nodes examined. LNPatient Patient's Sample Cancer Age (yr) Histological Tumor TNM AJCC LNID Diagnosis Sex Ethnicity Diagnosis size (cm) Stage Stage Mets LNpos5metastatic F 65 infiltrating 4.3 × 3 × 2.2 T2N2M0 IIIA  7/10 Caucasianductal carcinoma LNpos6 metastatic F 73 infiltrating 4 × 2 × 3.5 T4bN2M0IIIB 11/11 Caucasian ductal carcinoma LNpos7 metastatic F 54infiltrating 3 × 2.3 × 1.5 T2N1M0 IIB  2/22 Caucasian ductal carcinomaLNpos8 metastatic F 44 infiltrating 4.5 × 4.5 × 2.3 T2N2M0 IIIA 12/18Caucasian ductal carcinoma LNpos9 metastatic F 44 infiltrating 5 × 3 ×4.5 T2N2M0 IIIA  9/16 Caucasian ductal carcinoma LNpos10 metastatic F 42infiltrating 2.5 × 3.2 × 1.6 T2N1M0 IIB  3/13 Caucasian ductal carcinomaLNpos11 metastatic F 69 infiltrating 5.5 × 3 × 2.5 T3N2M0 IIIA 13/37Caucasian ductal carcinoma LNpos12 metastatic F 44 infiltrating 5 × 4.5× 4 T2N2M0 IIIA  6/13 Caucasian ductal carcinoma LNpos13 metastatic F 54infiltrating 1.8 × 3 × 1; T2N2M0 IIIA  7/15 Caucasian ductal 5 × 3.7 ×3.5 carcinoma LNpos14 metastatic F 46 infiltrating 2.8 × 2.5 × 2.7T2N1M0 IIB 2/5 Caucasian ductal carcinoma LNneg5 normal F 50infiltrating 3 T2N0M0 IIA 0/9 Caucasian ductal carcinoma LNneg6 normal F50 infiltrating 2.3 T2N1M0 IIB 1/4 Caucasian ductal carcinoma LNneg7normal F 60 infiltrating N/A T1N1M0 IIA 2/6 Caucasian ductal carcinomaLNneg8 normal F 35 infiltrating 2.5 T2N1M0 IIB  2/12 Caucasian ductalcarcinoma LNneg9 normal F 49 infiltrating 3.2 × 3 × 2.3 T2N1M0 IIB 2/9Caucasian ductal carcinoma

Total RNA isolation from the FFPE lymph node samples was performed usingthe RecoverAll™ Total Nucleic Acid Isolation Kit (cat. No. AM1975;Ambion, Inc.; Austin, Tex., USA), as described above in Example 1 andanalyzed on miRNA microarrays, as described below.

These samples were evaluated using the Agilent Human miRNA Microarray(V2) (Agilent Technologies, Inc.; Santa Clara, Calif. USA), whichcontains probes for 723 human and 76 human viral microRNAs from themiRBase database V.10.1 (Griffiths-Jones et al., 2006). Arrayhybridization, washing, staining, imaging, and signal extraction wereperformed according to Agilent's recommended procedures, as explainedbelow.

miRNA array expression analysis: Samples for miRNA profiling studieswere processed by Asuragen Services (Asuragen, Inc.; Austin, Tex., USA).Total RNA from each sample (100 ng) was dephosphorylated and a pCp-Cy3labeling molecule was ligated to the 3′ end of the RNA molecules,following the manufacturer's recommendations (Agilent Technologies, Inc.Santa Clara, USA). The labeled RNA was purified using a Bio-Spin P-6column (Bio-Rad Laboratories Inc.; Hercules, Calif., USA).

miRNA array signal processing: The signal processing implemented for theAgilent miRNA array is a multi-step process involving probe specificsignal detection calls, background correction, and global normalization.For each probe, the contribution of signal due to background wasestimated and removed by the Agilent Feature Extraction software as partof the data file output. Similarly, detection calls were based on theAgilent Feature Extraction software. Arrays within a specific analysisexperiment were normalized together according to the VSN methoddescribed elsewhere (Huber et al., 2002).

Background estimate and correction and probe detection: Three types ofdata are provided to evaluate each hybridization. The “Total GeneSignal” is the total probe signal multiplied by the number of probes pergene and is calculated after the background effects have been accountedfor. The “Total Gene Error” is the square root of the square of thetotal probe error multiplied by the number of probes per gene. The“Total Probe Error” is the robust average for each replicated probemultiplied by the total number of probe replicates. The “Detection Call”is a binary number that indicates if the gene was detected on the miRNAmicroarray. Probes detected at least once across all samples in theexperiment were considered for statistical analysis.

Global normalization: The inventors have found that the VarianceStabilization Normalization (VSN) algorithm provides an ideal balance ofaccuracy and precision while optimizing sensitivity and specificity ofsignal. One advantage of VSN, is that it accommodates negative values byusing the generalized log₂ transformation.

Generalized log₂ transformed: The post-normalized data scale is reportedas generalized log₂ data. The distribution of microarray data istypically log normal (i.e., it tends to follow a normal distributionpattern after log transformation). A normal distributed data isamendable to classical statistical treatments, including t-tests andone-way or two-way ANOVA.

For statistical hypothesis testing, a two-sample t-test, with assumptionof equal variance, was applied. This test is used to define which probesare considered to be significantly differentially expressed, or“significant”, based on false discovery rate set at 0.05.

LNpos5-14 and LNneg5-9 lymph node specimens from breast cancer patientswere evaluated. A total of 204 human miRNAs were expressed abovebackground level in the LNpos samples, representing 28% of the humanmiRNAs present on the arrays. A total of 176 human miRNAs were expressedabove background level in the LNneg samples, representing 24% of thehuman miRNAs present on the arrays.

A total of 61 miRNAs were significantly differentially expressed betweenLNpos and LNneg samples (Table 43). Among these, 54 miRNA had averageexpression levels higher in LNpos compare to LNneg samples. Three miRNAs(hsa-miR-200c, -141, and -200b) had average expression levels between170- and 400-fold higher in LNpos, six miRNAs (hsa-miR-183, -200a, -375,-429, -205, and -96) had average expression levels between 20- and65-higher in LNpos, and nine miRNAs (hsa-miR-196a, -21, -200a*, -21*,-210, -200b*, -503, -301a, -and -425) had average expression levelsbetween 5- and 10-fold higher in LNpos. A total of three miRNAs(hsa-miR-150, -139-5p, and -139-3p) had average expression levelsbetween 2- and 5-fold lower in LNpos than in LNneg samples.

TABLE 43 miRNAs differentially expressed between five cancer-negative(LNneg5-9) lymph nodes and ten cancer-positive (LNpos5-14) lymph nodesfrom breast cancer patients, following microarray expression analysis.Probe ID LNneg LNpos miRNA 5 6 7 8 9 Mean SD % 5 6 7 8 9 hsa-miR-200c−1.5 −2.5 −2.2 2.46 −1.4 −1.0 2.01 20 7.41 8.41 9.27 7.41 7.82hsa-miR-141 −0.7 −1.8 −1.0 1.32 −0.4 −0.5 1.16 0 7.49 7.98 9.42 7.007.15 hsa-miR-200b −0.5 −2.3 −0.6 2.25 −1.0 −0.4 1.68 20 7.99 8.53 7.405.66 6.00 hsa-miR-183 −0.6 0.12 −0.9 0.43 −0.3 −0.2 0.56 0 5.52 6.057.75 4.73 4.06 hsa-miR-200a −2.1 −1.5 −0.8 −0.9 −0.7 −1.2 0.57 0 5.246.72 4.25 3.16 3.57 hsa-miR-375 −2.0 −2.2 −2.2 1.21 −2.2 −1.5 1.52 05.21 5.64 1.68 2.42 3.98 hsa-miR-429 −1.6 −0.9 −0.8 −0.4 −1.1 −1.0 0.420 4.64 5.87 4.34 3.13 2.77 hsa-miR-205 −0.1 −1.0 −0.8 1.36 −0.2 −0.10.95 0 5.37 5.33 −0.2 5.45 0.56 hsa-miR-96 −0.5 0.90 0.46 0.23 0.79 0.370.57 0 4.60 4.78 6.14 3.98 2.21 hsa-miR-196a 0.97 1.25 2.73 2.84 0.741.71 1.00 40 6.11 6.45 6.65 6.29 0.98 hsa-miR-21 8.46 7.47 7.90 8.058.68 8.11 0.47 100 11.7 11.6 11.1 12.3 10.9 hsa-miR- −0.5 −1.8 −2.1 −0.5−1.0 −1.2 0.74 0 2.96 3.89 2.82 1.23 1.56 200a* hsa-miR-21* 0.86 −1.00.59 1.17 1.54 0.63 1.00 0 4.00 3.56 3.49 4.81 4.65 hsa-miR-210 2.251.76 2.34 2.70 2.74 2.36 0.40 80 6.76 6.61 6.46 3.99 5.26 hsa-miR- −0.9−2.4 −1.5 −1.1 −1.8 −1.5 0.59 0 1.62 3.28 1.60 0.63 0.70 200b*hsa-miR-503 −1.3 −1.2 −1.6 −1.0 −1.1 −1.2 0.22 0 1.68 1.36 0.91 3.311.04 hsa-miR-301a 0.21 −0.4 −0.1 0.18 0.21 0.02 0.28 0 3.54 4.39 4.854.42 2.70 hsa-miR-425 3.05 2.79 2.29 2.95 3.01 2.82 0.31 100 5.04 4.754.82 5.92 5.65 hsa-miR-193b 3.21 2.91 2.97 3.64 3.34 3.21 0.29 100 5.937.23 6.08 5.40 5.83 hsa-miR-130b 3.06 1.91 2.36 2.88 3.20 2.68 0.54 1004.82 4.89 6.51 4.74 5.07 hsa-miR-151 1.76 1.30 1.61 2.14 2.06 1.77 0.3440 4.74 4.32 5.15 3.50 4.56 3p hsa-miR-203 −0.6 −0.9 −1.3 −1.0 −0.2 −0.80.41 0 2.67 1.92 0.61 1.41 −0.3 hsa-miR-93 3.47 2.28 2.56 3.29 3.43 3.010.55 100 5.13 5.33 6.47 5.04 5.17 hsa-miR-454 0.02 −1.3 −1.0 −0.2 0.45−0.4 0.73 0 2.44 2.07 2.41 3.19 1.18 hsa-miR-106b 5.60 4.29 4.70 5.245.44 5.06 0.54 100 6.76 7.57 8.18 7.38 7.15 hsa-miR-324- 3.00 1.38 2.522.81 2.86 2.52 0.66 80 4.48 4.76 5.98 4.46 5.05 5p hsa-miR- 2.62 1.732.78 2.52 2.02 2.33 0.44 80 4.47 5.61 4.44 4.52 4.15 193a-3p hsa-miR-226.51 5.44 6.27 6.30 6.85 6.27 0.52 100 8.62 7.82 7.79 8.37 8.35hsa-miR-182 −1.2 −2.2 −2.0 −1.3 −2.1 −1.8 0.46 0 −0.61 −0.4 1.66 −0.2−0.5 hsa-miR-99b 1.88 1.38 1.69 2.06 1.86 1.77 0.25 20 2.15 4.42 3.474.08 3.98 hsa-miR-24 6.51 5.91 6.50 6.48 6.56 6.39 0.27 100 8.37 8.008.54 8.12 8.56 hsa-miR- 3.31 2.80 3.09 3.44 3.28 3.18 0.25 100 3.50 5.494.45 5.04 4.85 125a-5p hsa-miR-27a 6.30 5.39 6.71 6.17 6.06 6.12 0.48100 8.07 8.03 7.63 7.89 7.29 hsa-miR-331- 5.28 5.14 4.81 5.33 5.51 5.210.26 100 7.20 7.45 6.81 7.22 6.68 3p hsa-miR-18b −0.3 −0.7 −1.1 −1.0−0.5 −0.7 0.34 0 0.66 0.46 3.01 1.87 −0.35 hsa-miR-424 1.32 0.56 0.811.18 0.67 0.91 0.33 0 2.03 2.84 1.40 3.64 1.44 hsa-let-7e 6.18 5.50 5.946.29 6.13 6.01 0.31 100 6.53 8.27 7.29 7.95 7.78 hsa-miR-24- −1.2 0.36−0.7 −0.7 −0.8 −0.6 0.59 0 1.30 0.40 2.07 0.11 1.44 1* hsa-miR-148b 0.220.24 −0.9 0.50 −0.4 −0.1 0.60 0 1.44 2.28 1.00 1.83 1.18 hsa-miR-34a5.63 5.46 6.04 6.00 6.61 5.95 0.44 100 8.84 7.91 6.60 7.79 6.89hsa-miR-103 6.15 5.23 5.40 5.84 6.21 5.77 0.44 100 7.03 8.70 7.49 7.817.54 hsa-miR-107 5.56 4.69 4.75 5.24 5.59 5.17 0.43 100 6.32 7.79 7.547.06 7.03 hsa-miR-27b 4.35 3.63 4.77 4.28 3.98 4.20 0.43 100 5.77 5.306.20 5.38 5.94 hsa-miR- 2.59 1.27 2.58 3.07 2.60 2.42 0.68 100 4.36 4.793.44 4.04 3.98 193a-5p hsa-miR-214 4.11 3.66 3.40 3.85 3.87 3.78 0.27100 4.75 5.81 4.25 5.93 4.89 hsa-miR- 1.68 1.58 1.28 1.86 1.28 1.54 0.2620 3.05 3.40 1.94 3.80 1.62 199b-5p hsa-miR-30d 4.85 4.48 4.66 4.97 5.054.80 0.23 100 6.61 6.57 6.47 5.58 6.06 hsa-miR-629* 3.08 1.10 2.05 2.331.35 1.98 0.79 40 3.00 3.57 3.20 3.33 3.43 hsa-miR-15a 6.37 5.12 5.915.99 6.33 5.94 0.50 100 7.52 7.34 7.29 7.68 5.91 hsa-miR-23b 4.80 4.285.13 4.91 4.82 4.79 0.31 100 6.20 5.62 6.37 5.77 6.30 hsa-miR-25 5.605.08 4.54 5.26 5.51 5.20 0.42 100 6.21 6.14 7.07 6.89 5.93 hsa-miR- 6.045.17 5.51 5.54 5.59 5.57 0.31 100 6.83 7.04 5.67 7.93 5.99 199b-3phsa-miR-15b 7.59 6.87 6.70 7.20 7.34 7.14 0.36 100 7.61 8.13 9.11 8.617.56 hsa-miR-23a 6.49 6.02 6.57 6.41 6.52 6.40 0.22 100 7.64 7.49 7.137.35 6.86 hsa-miR-1227 2.26 2.44 2.35 2.52 2.43 2.40 0.10 100 1.62 2.131.75 1.92 2.05 hsa-miR-636 2.51 2.37 2.12 2.50 2.29 2.36 0.16 80 1.532.27 1.95 1.93 1.93 hsa-miR-145 4.87 4.36 5.10 5.04 4.91 4.86 0.29 1003.87 3.95 3.89 4.59 3.75 hsa-miR-126 6.88 5.68 6.65 6.24 6.36 6.36 0.45100 5.40 5.65 5.44 6.65 5.58 hsa-miR-139 2.28 2.38 3.11 1.81 2.29 2.370.47 100 0.53 1.61 1.53 2.46 1.62 3p hsa-miR-139 1.47 1.12 1.88 1.541.51 1.50 0.27 40 −2.34 0.91 0.44 0.20 −0.35 5p hsa-miR-150 10.0 9.578.48 10.0 10.1 9.66 0.70 100 7.20 7.15 5.84 10.5 7.93 Probe ID LNposLNpos vs LNneg miRNA 10 11 12 13 14 Mean SD % test Log2Diff FChsa-miR-200c 5.94 7.21 8.19 6.10 8.82 7.66 1.08 100 5.41E−08 8.70 416hsa-miR-141 5.97 7.14 7.76 5.63 8.59 7.41 1.13 100 9.79E−09 7.96 248hsa-miR-200b 5.33 7.83 7.33 5.95 8.18 7.02 1.17 100 1.53E−07 7.49 179hsa-miR-183 3.99 4.68 6.37 5.74 8.31 5.72 1.46 100 8.62E−07 6.00 63.9hsa-miR-200a 2.13 5.83 4.35 2.71 5.67 4.36 1.49 100 2.32E−06 5.59 48.3hsa-miR-375 4.10 6.39 −0.8 2.87 6.21 3.77 2.28 90 4.63E−04 5.27 38.7hsa-miR-429 1.49 4.36 3.19 2.41 5.22 3.74 1.36 80 4.66E−06 4.74 26.7hsa-miR-205 4.56 4.06 6.53 4.47 6.35 4.24 2.31 80 1.36E−03 4.42 21.4hsa-miR-96 4.12 4.08 5.45 4.62 7.23 4.72 1.35 100 1.26E−05 4.35 20.4hsa-miR-196a 4.78 7.04 5.41 4.27 9.43 5.74 2.18 90 1.90E−03 4.04 16.4hsa-miR-21 11.2 10.5 11.8 11.2 12.4 11.5 0.60 100 6.49E−08 3.39 10.5hsa-miR- 0.51 3.77 1.65 0.47 2.74 2.16 1.25 80 1.04E−04 3.37 10.3 200a*hsa-miR-21* 4.33 2.40 3.73 3.48 4.76 3.92 0.75 100 6.85E−06 3.30 9.8hsa-miR-210 5.60 2.75 6.48 6.31 6.28 5.65 1.32 100 1.29E−04 3.29 9.8hsa-miR- −0.3 2.93 1.51 0.18 1.87 1.40 1.15 80 1.22E−04 2.99 7.9 200b*hsa-miR-503 0.87 −0.4 1.31 2.03 4.10 1.62 1.28 80 2.71E−04 2.91 7.5hsa-miR-301a 1.00 0.75 2.64 1.22 2.95 2.85 1.49 70 1.16E−03 2.83 7.1hsa-miR-425 5.27 4.34 6.12 3.62 6.04 5.16 0.81 100 3.44E−05 2.34 5.1hsa-miR-193b 3.34 5.13 5.10 4.47 5.37 5.39 1.03 100 5.40E−04 2.17 4.5hsa-miR-130b 5.81 2.58 4.43 3.97 5.31 4.81 1.05 100 1.05E−03 2.13 4.4hsa-miR-151 2.34 2.79 3.83 2.43 5.01 3.87 1.06 100 9.61E−04 2.09 4.3 3phsa-miR-203 0.76 1.87 −0.1 0.76 1.23 1.08 0.93 20 7.55E−04 1.93 3.8hsa-miR-93 3.75 3.45 5.14 4.78 4.97 4.92 0.84 100 4.92E−04 1.92 3.8hsa-miR-454 −0.3 0.23 1.30 0.22 1.90 1.46 1.14 50 5.28E−03 1.89 3.7hsa-miR-106b 6.09 5.81 6.75 6.33 6.91 6.89 0.71 100 2.27E−04 1.84 3.6hsa-miR-324- 2.68 3.32 4.18 3.29 4.87 4.31 0.98 100 2.79E−03 1.79 3.5 5phsa-miR- 3.69 3.15 3.82 3.08 4.33 4.13 0.74 100 2.75E−04 1.79 3.5193a-3p hsa-miR-22 7.78 7.87 7.55 7.82 8.22 8.02 0.34 100 2.82E−06 1.753.4 hsa-miR-182 −1.3 −1.1 0.95 −1.2 1.84 −0.1 1.17 30 8.85E−03 1.70 3.2hsa-miR-99b 2.87 2.60 3.82 2.43 4.24 3.41 0.83 90 9.61E−04 1.63 3.1hsa-miR-24 7.17 7.50 7.78 7.74 8.33 8.01 0.46 100 7.30E−06 1.62 3.1hsa-miR- 4.76 4.27 6.04 4.18 5.23 4.78 0.72 100 4.08E−04 1.60 3.0125a-5p hsa-miR-27a 7.09 7.70 7.95 7.59 7.92 7.72 0.32 100 3.44E−06 1.593.0 hsa-miR-331- 5.80 5.96 7.04 5.80 7.98 6.79 0.74 100 5.20E−04 1.583.0 3p hsa-miR-18b 1.01 −0.66 0.39 0.58 1.18 0.82 1.06 40 6.81E−03 1.583.0 hsa-miR-424 1.66 1.59 2.65 2.78 4.14 2.42 0.96 50 4.90E−03 1.51 2.8hsa-let-7e 6.62 7.24 8.52 6.85 7.98 7.50 0.70 100 5.76E−04 1.50 2.8hsa-miR-24- 0.58 0.43 0.26 0.59 1.48 0.87 0.66 20 8.65E−04 1.50 2.8 1*hsa-miR-148b −0.2 1.36 1.89 0.79 2.46 1.40 0.79 40 2.69E−03 1.49 2.8hsa-miR-34a 7.10 6.87 7.24 7.33 7.39 7.40 0.65 100 6.35E−04 1.45 2.7hsa-miR-103 5.53 6.51 7.93 6.01 7.39 7.19 0.95 100 7.64E−03 1.43 2.7hsa-miR-107 5.03 5.69 7.36 5.46 6.63 6.59 0.94 100 7.26E−03 1.42 2.7hsa-miR-27b 4.94 5.23 5.10 5.63 5.98 5.54 0.42 100 6.26E−05 1.34 2.5hsa-miR- 3.42 3.47 3.67 2.86 3.46 3.75 0.55 100 1.32E−03 1.33 2.5193a-5p hsa-miR-214 4.07 5.80 5.25 4.55 5.63 5.09 0.69 100 1.33E−03 1.312.5 hsa-miR- 2.38 3.08 2.70 2.51 2.89 2.74 0.66 100 1.89E−03 1.20 2.3199b-5p hsa-miR-30d 5.06 5.34 6.64 4.80 6.44 5.96 0.70 100 3.78E−03 1.152.2 hsa-miR-629* 3.00 2.21 3.34 2.39 3.38 3.08 0.45 100 4.05E−03 1.102.1 hsa-miR-15a 6.30 7.19 7.28 6.36 7.14 7.00 0.59 100 4.70E−03 1.06 2.1hsa-miR-23b 5.20 5.72 5.29 5.80 5.95 5.82 0.40 100 2.18E−04 1.03 2.0hsa-miR-25 5.73 4.94 6.11 6.31 6.90 6.22 0.63 100 6.47E−03 1.02 2.0hsa-miR- 5.81 7.12 6.37 6.30 6.75 6.58 0.69 100 8.96E−03 1.01 2.0199b-3p hsa-miR-15b 8.03 7.95 7.83 7.34 7.94 8.01 0.52 100 5.28E−03 0.871.8 hsa-miR-23a 6.36 7.17 7.67 6.96 7.21 7.18 0.40 100 1.34E−03 0.78 1.7hsa-miR-1227 2.42 1.49 2.12 2.13 2.23 1.99 0.29 70 9.67E−03 −0.4 1.3hsa-miR-636 2.14 1.33 2.01 1.37 2.03 1.85 0.32 70 6.00E−03 −0.5 1.4hsa-miR-145 4.03 4.02 4.67 4.06 3.68 4.05 0.33 100 4.69E−04 −0.8 1.7hsa-miR-126 5.23 5.19 5.40 5.11 5.86 5.55 0.45 100 5.63E−03 −0.8 1.8hsa-miR-139 1.34 0.92 0.09 1.23 1.06 1.24 0.65 40 4.38E−03 −1.1 2.2 3phsa-miR-139 0.01 0.81 0.59 −0.88 0.65 0.01 0.99 0 6.22E−03 −1.5 2.8 5phsa-miR-150 7.42 6.79 6.45 5.75 7.89 7.30 1.38 100 3.51E−03 −2.3 5.1 SD,standard deviation; %, percentage of samples expressing miRNA abovebackground levels; Log2Diff, difference in VSN-transformed expressionbetween LNpos and LNneg samples; FC, fold change.

Example 45 qRT-PCR Verification of Differentially Expressed Micro-RNAsin Two Group of Cancer-positive and Cancer-negative Lymph Nodes fromBreast Cancer Patients

The inventors evaluated the differential expression of ten selectedmiRNAs (miR-429, -200a, -375, -200b, -203, -141, -200c, -183, -196a, and-205) in LNpos and LNneg samples from breast cancer patients (Table 38,Example 40 and Table 42, Example 44), by qRT-PCR. qRT-PCR reactions wereperformed using Taqman® MicroRNA Assays (Applied Biosystems; FosterCity, Calif., USA) specific for the ten microRNAs. Assays were performedaccording to the manufacturer's instructions with 15 ng of input totalRNA from the samples (LNpos2-4, LNpos5-14 and LNneg1-9). Reactions wereincubated in a 7900HT Real-Time PCR system (Applied Biosystems, FosterCity, Calif., USA). Initial data analysis was done using the 7500 FastSystem SDS V2.3 software (Applied Biosystems). With this combined set ofsamples, miR-638 expression level was found to be more variable acrossthe samples than in qRT-PCR reactions performed with only samples fromTable 38 (Example 43). Here, the miR-16 expression level was constantamong the different samples. As a consequence, the inventors chose touse the mean of the sum of the Ct values for both miR-16 and miR-638(1/2(Ct miR-16+Ct miR-638)) for normalization (dCt=Ct miRNA -1/2(CtmiR-16+Ct miR-638). Average expression levels of each miRNA in LNneg andLNpos of the two independent sets of samples are illustrated afternormalization to miR-16 and miR-638 (FIG. 14A, FIG. 14B). qRT-PCRquantification, using miR-16 and miR-638 as normalizers, confirmed thedifferential expression of the ten miRNAs in the LNneg1-4 and LNpos2-4samples, (FIG. 14A), as well as in LNpos 5-14 and LNneg 5-9 (FIG. 14B),validating their differential expression in LNneg and LNpos from breastcancer patients. Combined qRT-PCR data for the two sets of samples(LNpos2-14 and LNneg1-9) (FIG. 15) showed that seven miRNAs inparticular, miR-429, -200a, -200b, -203, -141, -200c, and -375,represent useful markers for the identification of breast cancer cellsin axillary lymph nodes of breast cancer patients.

Example 46 Identification of Classifiers for Cancer-positive Lymph Nodesin Patients with Ductal Breast Cancer

The inventors have demonstrated that miRNA quantification and expressionanalysis can differentiate cancer-positive from cancer-negative axillarylymph nodes from breast cancer patients. To further demonstrate theutility of specific miRNA markers as diagnostic analytes, the inventorsquantified specific combinations of miRNAs by qRT-PCR to determine ifany such combinations enhanced the distinction between cancer-positiveand cancer-negative lymph nodes from breast cancer patients. qRT-PCRassays were performed as described above in Example 45. For the miRNAcombinations miR-429+miR-200a, miR-429+miR-141, miR-200b+miR-203, thenormalized Ct values to both miR-16 and miR-638 were added. For thecombinations, miR-429-miR-150 and miR-200a-miR-150, the raw Ct values ofeach miRNA were subtracted. The inventors determined that quantificationof several miRNA combinations such as miR-429+miR-200a, miR-429+miR-141,miR-200b+miR-203, miR-429-miR-150, and miR200a-miR-150, enhances thedistinction between cancer-positive and cancer-negative lymph nodes frombreast cancer patients (FIG. 16).

Example 47 Additional Microarray Analysis of MicroRNA DifferentiallyExpressed Between Cancer-positive and Cancer-negative Inguinal LymphNodes from Cervical Cancer Patients

Further microRNA microarray expression analysis was performed on theLNpos and LNneg inguinal lymph nodes from cervical cancer patients thathave been described above in Example 1, Table 3. Here, the microRNAarray used for expression analysis was the Agilent Human miRNAMicroarray (V2) (Agilent Technologies, Inc.; Santa Clara, Calif., USA),described above in Example 44. Microarray expression analysis was asdescribed above in Example 44.

A total of 324 human miRNAs were expressed above background level in theLNpos samples, representing 45% of the miRNAs present on the arrays. Atotal of 260 miRNAs were expressed above background level in the LNnegsamples, representing 36% of the miRNAs present on the arrays.

A total of 107 human miRNAs were significantly differentially expressedbetween LNpos and LNneg (Table 44). Among these, 57 were up-regulated(Log2 diff (LNpos vs LNneg)≧1) and 40 were down-regulated (Log2 diff(LNpos vs LNneg)≦1) by more than 2-fold in LNpos as compared with LNnegsamples. Twenty-two miRNAs were expressed at levels at least 5-foldhigher (Log2 diff (LNpos vs LNneg)<2.3) in the LNpos samples, with aStudent t-test p-value<0.01. Of those, 10 miRNAs (hsa-miR-205, -200b,-203, -200a, -429, -200c, -141, -224, -135b, and -200b*) were expressedat levels at least 10-fold higher in the LNpos samples (Log2 diff (LNposvs LNneg)≧3.3). Among the miRNAs expressed at lower levels in the LNpossamples, only one (hsa-miRNA-342-5p) was under-expressed by at least5-fold compared to the LNneg samples, with a Student t-testp-value≦0.01.

TABLE 44 miRNAs differentially expressed between four cancer-positive(LNpos) lymph nodes and four cancer-negative (LNneg) lymph nodes fromcervical cancer patients following microarray expression analysis. ProbeID LNneg LNpos LNpos vs LNneg miRNA 1 2 3 4 Mean SD % 1 2 3 4 Mean SD %ttest Log 2 Diff FC hsa-let-7c 12.90 12.39 12.52 12.63 12.61 0.22 10013.38 13.72 12.95 13.52 13.39 0.33 100 7.13E−03 0.78 2 hsa-let-7g 14.1814.35 13.65 13.90 14.02 0.31 100 12.86 11.97 12.19 13.47 12.62 0.68 1009.60E−03 −1.40 3 hsa-let-7i 13.37 13.38 12.90 13.08 13.18 0.23 100 12.4112.21 12.62 12.73 12.49 0.23 100 5.70E−03 −0.69 2 hsa-miR-1225-3p 4.914.83 5.39 5.04 5.04 0.25 100 4.23 4.40 4.45 4.40 4.37 0.10 100 2.38E−03−0.67 2 hsa-miR-1238 4.90 4.89 5.01 4.81 4.90 0.08 75 4.12 4.01 4.444.23 4.20 0.18 50 4.05E−04 −0.70 2 hsa-miR-124 4.43 4.96 6.19 4.26 4.960.87 100 3.67 3.34 3.30 3.33 3.41 0.17 25 1.30E−02 −1.55 3 hsa-miR-12613.11 12.37 11.98 12.57 12.51 0.47 100 11.03 10.87 10.58 11.60 11.020.43 100 3.52E−03 −1.49 3 hsa-miR-127-3p 3.83 4.72 4.14 4.43 4.28 0.3825 6.20 6.85 7.19 5.16 6.35 0.89 100 5.26E−03 2.07 4 hsa-miR-129-3p 5.674.93 4.93 4.58 5.03 0.46 100 3.09 2.87 3.21 3.77 3.23 0.38 0 9.48E−04−1.79 3 hsa-miR-135b 2.52 3.25 2.57 3.24 2.90 0.40 0 7.99 5.27 6.85 6.376.62 1.13 100 7.94E−04 3.73 13 hsa-miR-138-2* 5.40 5.15 5.37 5.19 5.280.13 100 6.25 6.09 5.93 6.11 6.09 0.13 100 1.10E−04 0.82 2hsa-miR-139-3p 6.64 6.94 6.71 6.26 6.64 0.28 100 5.96 6.01 5.71 6.115.95 0.17 100 5.73E−03 −0.69 2 hsa-miR-139-5p 7.65 6.84 7.16 7.41 7.260.35 100 5.98 5.95 4.90 5.72 5.63 0.50 100 1.80E−03 −1.63 3hsa-miR-140-3p 11.04 11.11 10.60 10.93 10.92 0.23 100 9.42 8.82 8.6910.05 9.25 0.62 100 2.30E−03 −1.68 3 hsa-miR-140-5p 8.51 8.50 7.79 8.398.30 0.35 100 7.05 6.63 6.85 7.67 7.05 0.45 100 4.53E−03 −1.25 2hsa-miR-141 4.62 4.46 3.29 4.40 4.19 0.61 50 10.08 10.75 11.55 10.0210.60 0.71 100 9.59E−06 6.41 85 hsa-miR-141* 1.43 0.99 0.60 1.51 1.130.42 0 3.53 3.71 3.89 2.90 3.51 0.43 50 2.19E−04 2.38 5 hsa-miR-149 4.204.08 4.58 4.19 4.26 0.22 25 6.99 7.84 5.03 6.52 6.59 1.18 100 7.96E−032.33 5 hsa-miR-16-2* 4.63 4.94 4.69 4.58 4.71 0.16 100 5.63 5.75 5.955.89 5.81 0.14 100 5.29E−05 1.10 2 hsa-miR-181a 10.58 10.66 10.29 10.9210.61 0.26 100 9.25 9.36 9.58 9.94 9.53 0.30 100 1.65E−03 −1.08 2hsa-miR-181b 8.48 8.54 8.46 8.95 8.61 0.23 100 7.77 7.68 7.85 8.29 7.900.27 100 7.17E−03 −0.71 2 hsa-miR-182 1.89 2.57 1.72 2.86 2.26 0.55 05.44 4.79 5.06 4.25 4.88 0.50 100 3.98E−04 2.62 6 hsa-miR-183 2.82 4.012.34 3.65 3.20 0.76 25 6.74 6.07 7.03 5.82 6.42 0.56 100 5.02E−04 3.21 9hsa-miR-183* 2.71 1.84 2.55 1.65 2.19 0.52 0 3.28 3.29 3.78 3.07 3.350.30 25 8.29E−03 1.17 2 hsa-miR-191* 4.70 4.68 4.67 4.41 4.62 0.14 254.03 3.95 4.38 4.06 4.11 0.19 25 4.71E−03 −0.51 1 hsa-miR-192 7.48 7.616.65 7.12 7.22 0.43 100 6.01 5.16 4.66 6.23 5.51 0.73 100 6.99E−03 −1.703 hsa-miR-194 6.74 6.71 5.91 6.56 6.48 0.39 100 5.84 5.04 4.66 5.60 5.290.54 100 1.14E−02 −1.19 2 hsa-miR-195 11.90 11.51 10.80 11.60 11.45 0.47100 10.33 10.11 10.45 10.68 10.39 0.24 100 6.68E−03 −1.06 2 hsa-miR-1977.52 7.29 7.90 7.91 7.65 0.30 100 6.66 5.99 6.23 6.69 6.39 0.34 1001.48E−03 −1.26 2 hsa-miR-198 4.46 5.06 5.86 4.71 5.02 0.61 75 6.39 6.596.84 5.91 6.43 0.39 100 8.15E−03 1.41 3 hsa-miR-199b-5p 6.91 6.49 6.857.01 6.81 0.23 100 8.42 8.42 9.11 7.90 8.46 0.49 100 9.14E−04 1.65 3hsa-miR-200a 3.75 3.42 3.02 3.82 3.50 0.37 0 11.92 11.26 12.71 11.0011.72 0.76 100 1.21E−06 8.22 298 hsa-miR-200a* 2.74 2.99 3.64 3.19 3.140.38 0 6.48 6.60 6.59 5.28 6.24 0.64 100 1.62E−04 3.10 9 hsa-miR-200b4.54 4.11 2.95 3.52 3.78 0.69 25 12.82 11.90 12.83 11.42 12.24 0.70 1002.48E−06 8.46 352 hsa-miR-200b* 3.66 3.24 3.90 3.64 3.61 0.27 0 7.546.96 7.17 6.23 6.98 0.55 100 3.50E−05 3.37 10 hsa-miR-200c 4.77 4.484.70 4.38 4.58 0.18 100 10.77 11.27 11.80 10.28 11.03 0.65 100 1.35E−066.45 87 hsa-miR-203 2.73 1.80 2.30 1.63 2.11 0.50 0 7.51 10.76 12.5310.99 10.45 2.11 100 2.53E−04 8.34 323 hsa-miR-205 1.99 2.22 1.24 1.651.77 0.43 0 13.15 15.02 15.15 13.55 14.22 1.02 100 4.94E−07 12.44 5573hsa-miR-21 12.70 13.26 12.33 13.05 12.83 0.41 100 14.27 13.93 15.0814.24 14.38 0.49 100 2.82E−03 1.55 3 hsa-miR-21* 5.45 6.05 5.81 5.665.74 0.25 100 7.26 7.15 7.94 7.50 7.46 0.35 100 2.08E−04 1.72 3hsa-miR-210 6.42 6.70 6.15 6.72 6.50 0.27 100 9.25 9.77 9.71 9.49 9.550.24 100 2.50E−06 3.05 8 hsa-miR-215 6.59 6.64 5.86 6.29 6.35 0.36 1005.60 4.87 4.58 5.57 5.16 0.51 100 8.84E−03 −1.19 2 hsa-miR-22* 4.62 3.933.47 4.18 4.05 0.48 25 5.98 5.63 5.59 5.41 5.65 0.24 100 9.65E−04 1.60 3hsa-miR-221 8.26 7.90 7.77 7.77 7.93 0.23 100 9.00 9.30 9.99 9.23 9.380.43 100 9.53E−04 1.45 3 hsa-miR-224 6.33 5.82 5.41 5.34 5.73 0.45 10010.16 9.51 10.33 10.03 10.01 0.35 100 5.68E−06 4.28 19 hsa-miR-23a 12.9212.68 12.48 12.90 12.74 0.21 100 13.45 13.19 13.80 13.45 13.47 0.25 1004.19E−03 0.73 2 hsa-miR-23b 10.66 10.25 10.32 10.54 10.44 0.19 100 12.1512.96 12.92 13.09 12.78 0.43 100 5.89E−05 2.34 5 hsa-miR-23b* 2.58 2.383.18 3.84 3.00 0.66 0 4.11 5.37 4.42 5.07 4.74 0.58 100 7.26E−03 1.75 3hsa-miR-24 12.13 11.91 11.57 11.95 11.89 0.23 100 12.55 12.79 13.1113.03 12.87 0.25 100 1.26E−03 0.98 2 hsa-miR-24-1* 2.71 2.74 2.34 3.342.78 0.41 0 3.93 4.60 4.71 4.62 4.46 0.36 100 8.74E−04 1.68 3hsa-miR-26a 13.16 13.09 12.57 13.04 12.97 0.27 100 12.09 11.55 12.0012.26 11.98 0.30 100 2.71E−03 −0.99 2 hsa-miR-26b 13.07 12.84 12.3612.87 12.79 0.30 100 11.79 11.30 11.24 12.43 11.69 0.55 100 1.32E−02−1.10 2 hsa-miR-27b 9.95 9.57 9.35 9.60 9.62 0.25 100 11.25 12.07 12.1712.18 11.92 0.45 100 1.04E−04 2.30 5 hsa-miR-29a 14.46 14.57 13.72 13.9414.17 0.41 100 12.46 11.35 12.08 13.15 12.26 0.75 100 4.17E−03 −1.92 4hsa-miR-29c 13.74 13.47 12.52 12.82 13.14 0.56 100 11.62 10.51 10.7012.30 11.28 0.83 100 1.02E−02 −1.85 4 hsa-miR-30a 9.82 9.64 8.98 9.689.53 0.37 100 8.66 8.20 8.27 9.08 8.55 0.40 100 1.18E−02 −0.98 2hsa-miR-30b 11.41 11.25 10.54 11.01 11.05 0.38 100 10.16 9.96 10.2410.55 10.23 0.25 100 1.05E−02 −0.83 2 hsa-miR-30c-1* 4.04 4.11 4.09 4.094.08 0.03 100 4.27 4.47 4.46 4.25 4.36 0.12 100 3.83E−03 0.28 1hsa-miR-31* 2.22 4.08 3.58 2.66 3.14 0.85 0 6.20 4.99 5.21 6.21 5.650.64 100 3.24E−03 2.52 6 hsa-miR-324-3p 11.04 10.89 10.94 10.79 10.920.11 100 10.34 10.27 10.35 10.76 10.43 0.23 100 7.97E−03 −0.49 1hsa-miR-326 5.24 4.96 4.63 4.87 4.93 0.25 100 4.04 3.56 3.43 4.44 3.870.46 75 6.92E−03 −1.06 2 hsa-miR-328 5.34 5.46 5.64 5.78 5.56 0.20 1004.27 3.62 3.70 4.25 3.96 0.35 50 2.02E−04 −1.60 3 hsa-miR-338-3p 6.346.40 5.96 6.64 6.34 0.28 100 5.39 5.22 4.96 5.73 5.32 0.32 100 3.19E−03−1.02 2 hsa-miR-338-5p 5.05 4.95 5.35 5.28 5.16 0.19 100 4.45 4.75 4.614.60 4.60 0.12 100 2.49E−03 −0.56 1 hsa-miR-342-3p 12.34 12.76 12.1212.52 12.43 0.27 100 10.58 9.96 10.31 11.31 10.54 0.57 100 9.74E−04−1.90 4 hsa-miR-342-5p 9.27 9.65 9.40 9.53 9.46 0.16 100 7.54 6.55 6.418.47 7.24 0.96 100 3.84E−03 −2.22 5 hsa-miR-34a* 5.04 4.86 4.28 4.504.67 0.34 75 5.73 5.46 5.40 5.59 5.54 0.15 100 3.49E−03 0.87 2hsa-miR-370 3.95 4.35 4.73 4.28 4.33 0.32 0 5.59 6.24 5.80 4.94 5.640.54 100 5.90E−03 1.31 2 hsa-miR-376a 3.73 4.36 3.09 3.74 3.73 0.52 05.53 6.06 6.83 4.77 5.80 0.87 100 6.44E−03 2.07 4 hsa-miR-376c 3.49 4.533.81 4.15 4.00 0.45 0 5.38 5.85 6.96 4.98 5.79 0.86 100 9.82E−03 1.80 3hsa-miR-377 2.51 2.47 2.18 3.15 2.58 0.41 0 4.64 5.14 5.93 4.16 4.970.76 75 1.43E−03 2.39 5 hsa-miR-378 7.01 6.50 6.61 6.71 6.71 0.22 1008.64 8.58 8.85 8.09 8.54 0.32 100 7.92E−05 1.84 4 hsa-miR-378* 4.87 4.084.19 4.32 4.37 0.35 25 5.87 5.58 5.89 5.15 5.62 0.34 100 2.19E−03 1.26 2hsa-miR-381 3.02 3.05 3.41 2.85 3.08 0.23 0 4.23 4.16 4.74 3.52 4.160.50 25 8.16E−03 1.08 2 hsa-miR-409-3p 2.70 3.78 3.07 3.01 3.14 0.46 05.28 5.38 5.73 3.97 5.09 0.77 75 4.76E−03 1.95 4 hsa-miR-423-3p 5.125.03 5.03 4.91 5.02 0.09 100 5.45 5.43 5.24 5.22 5.34 0.12 100 6.07E−030.31 1 hsa-miR-425* 4.61 4.73 4.76 4.66 4.69 0.06 25 3.91 4.27 3.85 4.144.04 0.20 0 7.96E−04 −0.65 2 hsa-miR-429 2.43 1.51 2.13 1.20 1.82 0.56 010.25 9.04 10.81 8.88 9.74 0.94 100 6.72E−06 7.93 244 hsa-miR-432 3.273.28 3.89 3.51 3.49 0.29 0 4.24 4.77 4.75 3.87 4.41 0.43 50 1.27E−020.92 2 hsa-miR-452 4.51 4.61 4.11 4.17 4.35 0.24 25 6.58 5.57 6.74 6.156.26 0.53 100 5.86E−04 1.91 4 hsa-miR-486-3p 4.56 3.34 4.16 3.38 3.860.60 75 1.93 2.24 2.12 2.76 2.26 0.35 0 3.76E−03 −1.60 3 hsa-miR-486-5p7.17 7.37 6.77 7.16 7.12 0.25 100 6.30 6.56 6.35 6.76 6.49 0.21 1008.79E−03 −0.63 2 hsa-miR-487b 5.04 4.52 5.08 4.59 4.81 0.29 75 6.02 6.236.18 6.22 6.16 0.10 100 1.18E−04 1.35 3 hsa-miR-493* 2.79 3.07 2.56 2.622.76 0.23 0 4.01 3.99 4.76 3.44 4.05 0.54 75 4.59E−03 1.29 2 hsa-miR-49412.26 12.64 12.70 11.88 12.37 0.38 100 13.78 14.13 13.15 13.22 13.570.47 100 7.35E−03 1.20 2 hsa-miR-495 1.38 2.26 1.30 1.69 1.66 0.44 03.83 4.37 5.22 3.47 4.22 0.76 75 1.10E−03 2.56 6 hsa-miR-500* 6.95 7.126.94 6.86 6.97 0.11 100 6.57 6.23 6.20 6.45 6.36 0.18 100 1.16E−03 −0.612 hsa-miR-502-3p 6.36 6.23 6.12 6.06 6.19 0.13 100 6.01 5.78 5.67 5.915.84 0.15 100 1.26E−02 −0.35 1 hsa-miR-505 8.11 7.79 7.52 7.75 7.79 0.24100 6.86 6.30 5.83 7.02 6.50 0.55 100 5.00E−03 −1.29 2 hsa-miR-505* 6.095.96 5.89 5.96 5.98 0.08 100 5.11 4.85 4.04 5.26 4.81 0.54 100 5.46E−03−1.16 2 hsa-miR-513a-5p 7.78 8.21 8.62 7.32 7.98 0.56 100 9.60 9.86 9.409.15 9.50 0.30 100 3.03E−03 1.52 3 hsa-miR-513b 6.57 7.05 7.18 6.11 6.730.49 100 8.15 8.44 7.76 7.59 7.99 0.38 100 6.60E−03 1.26 2 hsa-miR-513c5.23 5.92 6.04 5.02 5.55 0.50 100 6.93 7.29 6.41 6.47 6.78 0.42 1009.48E−03 1.22 2 hsa-miR-532-3p 6.64 6.75 6.39 6.23 6.50 0.24 100 5.995.66 5.83 6.01 5.87 0.16 100 4.57E−03 −0.63 2 hsa-miR-532-5p 7.55 7.707.28 7.43 7.49 0.18 100 6.90 6.61 6.99 6.88 6.84 0.16 100 1.75E−03 −0.652 hsa-miR-625 7.52 7.55 7.46 7.17 7.43 0.17 100 6.36 5.21 6.04 6.82 6.110.68 100 9.38E−03 −1.32 2 hsa-miR-629* 4.72 4.69 4.82 5.10 4.83 0.19 1005.85 5.07 5.87 5.61 5.60 0.37 100 1.01E−02 0.77 2 hsa-miR-630 6.60 7.558.09 7.04 7.32 0.64 100 9.09 9.24 10.69 8.74 9.44 0.86 100 7.55E−03 2.124 hsa-miR-654-5p 4.44 4.63 4.89 4.32 4.57 0.25 100 5.19 5.37 5.10 5.005.17 0.16 100 6.57E−03 0.59 2 hsa-miR-768-3p 13.48 13.54 13.22 13.1713.35 0.19 100 12.09 11.17 10.86 12.44 11.64 0.75 100 4.35E−03 −1.71 3hsa-miR-769-5p 5.79 5.79 5.54 5.72 5.71 0.12 100 5.47 4.65 4.93 5.185.06 0.35 100 1.24E−02 −0.66 2 hsa-miR-96 3.55 4.40 2.79 4.13 3.72 0.710 7.00 6.60 7.43 6.35 6.84 0.47 100 3.31E−04 3.13 9 hsa-miR-98 8.07 7.847.57 7.85 7.83 0.20 100 8.52 8.38 8.93 8.78 8.65 0.25 100 2.16E−03 0.822 hsa-miR-99b* 4.12 3.82 4.38 4.31 4.16 0.25 0 4.83 4.89 4.73 4.47 4.730.19 100 1.06E−02 0.57 1 SD, standard deviation; %, percentage ofsamples expressing miRNA above background levels; Log2Diff, differencein VSN-transformed expression between LNpos and LNneg samples; FC, foldchange.

Example 48 ORT-PCR Verification of Differentially Expressed miRNAs UsingFFPE Melanoma Samples and FFPE Cancer-positive Lymph Nodes from MelanomaPatients

The inventors further evaluated differential expression of miRNAs usinga set of formalin-fixed, paraffin-embedded (FFPE) tissue samples thatincluded four cancer-positive lymph nodes (LNpos5-8) and three melanomasamples (MEL4-6) (Tables 8 and 9). These samples were independent anddistinct from the frozen melanoma biopsy samples (Table 8) and thefrozen cancer-positive lymph node samples (Table 9) previously evaluated(Examples 11, 12, and 13).

QRT-PCR quantification of 14 selected miRNAs was performed using TaqMangMicroRNA Assays (Applied Biosystems) as described in Example 13.Differential expression of the miRNAs was evaluated by comparing theexpression level of each miRNA in MEL4-6 (FIG. 17A) and in LNpos5-8(FIG. 17B) with their expression levels in LNneg1-7. These experimentsconfirmed the differential expression, between MEL4-6 and LNneg1-7, forthe 14 miRNAs (FIG. 17A). These experiments also verified thedifferential expression of miR-513 (FIGS. 17A,17B) which had beenpreviously identified as differentially expressed by array analysis(Table 10, Table 11), but which had not been evaluated by qRT-PCR. Inaddition, these experiments confirmed the differential expression ofseven of the 14 miRNAs between cancer-positive lymph nodes (LNpos5-8)and cancer-negative lymph nodes (LNneg1-7) (FIG. 17B). Thecancer-positive lymph node designated LNpos8 was an outlier for miR-211,the most differentially expressed miRNA of the fourteen evaluated here(FIG. 17B). In addition, LNpos8, like LNpos1A, had higher expression ofmiR-203 than was observed for the LNneg samples. However, thedifferential expression of the other miRNAs in LNpos8 vs LNneg1-7 wasconsistent with the pattern observed for the other cancer-positive lymphnodes (LNpos5-7, FIG. 17B).

QRT-PCR quantification of fourteen different miRNAs, in all 15 lymphnode samples from melanoma patients (Table 9) is summarized in FIG. 18.The inventors have identified two subgroups of cancer-positive lymphnodes (LNposA, LNposB) from melanoma patients. The LNposA subgroup isrepresented by a single cancer-positive lymph node—LNpos1A. The LNposBsubgroup is represented by the other cancer-positive lymph nodes(LNpos2-8). Within the LNposB subgroup LNpos8 is an outlier forhsa-miR-211 and hsa-miR-203 expression.

The inventors have shown that miRNA array profiling and qRT-PCR analysiscan distinguish between cancer-positive and cancer-negative lymph nodesfrom melanoma patients. The inventors have identified seven miRNAs(hsa-miR-211, -514, -506, -509, -508, -513, and -584) (FIG. 18) that areexpressed at significantly higher levels in all cancer-positive lymphnodes and melanoma samples than in cancer-negative lymph nodes. Theresults are consistent when analyses are performed with either frozenbiopsy specimens or FFPE tissue samples. These miRNAs representeffective markers for the identification of metastatic melanoma in thelymph nodes.

In addition, the inventors identified six miRNAs (hsa-miR-200b, -200c,-203, -375, -429, and -592) that are expressed at significantly higherlevels in the LNposA subgroup of cancer-positive lymph nodes (FIG. 8,FIG. 18) and in melanoma samples (FIG. 7, FIG. 17) compared tocancer-negative lymph nodes (LNneg). These six miRNAs were notsignificantly differentially expressed between the LNposB subgroup ofcancer-positive lymph nodes and cancer-negative lymph nodes (LNneg)(FIG. 18). These miRNAs represent useful diagnostic markers fordistinguishing between the two subgroups (LNposA, LNposB) ofcancer-positive lymph nodes in metastatic melanoma patients. Theheterogeneity observed in the miRNA expression profiles among thecancer-positive lymph nodes correlates with the fact that melanoma is aheterogeneous disease that involves multiple biological pathways.

Example 49 MicroRNA Combinations as Classifiers for Cancer-positiveLymph Nodes in Patients with Melanoma

The inventors have demonstrated that miRNAs can distinguishcancer-positive from cancer-negative lymph nodes taken from melanomapatients. To further refine the utility of specific microRNA markers asdiagnostic analytes, the inventors quantified specific combinations ofmiRNAs by qRT-PCR and identified combinations that enhanced thedistinction between cancer-positive and cancer-negative lymph nodes frommelanoma patients. qRT-PCR assays were performed as described above inExample 13. For combinations of two or more miRNAs, the Ct values fromthe quantification of individual miRNAs were added following theirnormalization to miR-16. The inventors determined that quantification ofseveral miRNA combinations (miR-211+miR-514; miR-211+miR-506;miR-211+miR-514+miR-506; miR-211+miR-509+miR-508) enhances thedistinction between cancer-positive and cancer-negative lymph nodes frommelanoma patients (FIG. 19). These combinations serve as non-limitingexamples. Other combinations may also be used and combinations mayinclude subtraction of average Ct values. In that case, if an evennumber of miRNAs is present in the combination, the subtraction of Ctvalues in a combination eliminates the need to normalize the data.

REFERENCES

The following references, to the extent that they provide exemplaryprocedural or other details supplementary to those set forth herein, arespecifically incorporated herein by reference.

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1. A method of assessing a patient comprising: detecting the presence ofa non-lymphoid cell in a lymph node sample by evaluating the expressionlevels of one or more miRNAs differentially expressed in non-lymphoidtissue as compared to lymph node tissue.
 2. The method of claim 1,further comprising providing a diagnosis, prognosis, or staging of thepatient condition or disease.
 3. The method of claim 1, wherein one ormore miRNA is selected from Table 4, 5, 6, 10, 11, 12, 13, 14, 16, 17,18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35,36, 37, 39, 40, 41, 43, and/or
 44. 4. The method of claim 3, furthercomprising combining the expression levels of two or more miRNAsselected from Table 4, 5, 6, 10, 11, 12, 13, 14, 16, 17, 18, 19, 20, 21,22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 39, 40,41, 43, and/or
 44. 5. The method of claim 1, wherein the lymph nodesample is associated with cancer or suspected cancer.
 6. The method ofclaim 5, wherein the lymph node sample is obtained from a lymph nodepositioned locally to a cancer or suspected cancer.
 7. The method ofclaim 6, wherein the lymph node is a sentinel lymph node (SLN). 8.(canceled)
 9. (canceled)
 10. The method of claim 1, wherein detection isby RT-PCR and/or hybridization.
 11. The method of claim 1, wherein thenon-lymphoid tissue is cervix, skin, colon, breast, bladder, esophagus,liver, ovary, prostate, lung, pancreas, thyroid, kidney, stomach,testicle, uterus, spleen, tongue, brain, thymus, trachea, smallintestine and/or a cancer originating from the non-lymphoid tissue.12-14. (canceled)
 15. The method of claim 1, wherein one or more miRNAsare selected from hsa-miR-182, hsa-miR-183, hsa-miR-189, hsa-miR-200a,hsa-miR-200b, hsa-miR-200c, hsa-miR-203, hsa-miR-205, hsa-miR-224,hsa-miR-429, hsa-miR-141, hsa-miR-375, hsa-miR-211, hsa-miR-509,hsa-miR-508, hsa-miR-514 hsa-miR-584, hsa-miR-592, hsa-miR-192,hsa-miR-194, or hsa-miR-215.
 16. The method of claim 15, furthercomprising combining the expression levels of two or more miRNAsselected from hsa-miR-182, hsa-miR-183, hsa-miR-189, hsa-miR-200a,hsa-miR-200b, hsa-miR-200c, hsa-miR-203, hsa-miR-205, hsa-miR-224,hsa-miR-429, hsa-miR-141, hsa-miR-375, hsa-miR-211, hsa-miR-509,hsa-miR-508, hsa-miR-514, hsa-miR-584, hsa-miR-592, hsa-miR-192,hsa-miR-194, or hsa-miR-215. 17-83. (canceled)
 84. A method of assessinga patient comprising the steps of: (a) evaluating a lymph node samplefor one or more miRNAs differentially expressed in two or morenon-lymphoid tissue as compared to lymph node tissue; and (b)determining if a non-lymphoid cell is present in the lymph node sampleby evaluating the expression levels of the differentially expressedmiRNAs.
 85. The method of claim 84, wherein the two non-lymphoid tissuesare selected from cervix, skin, colon, breast, bladder, esophagus,liver, ovary, prostate, lung, pancreas, thyroid, kidney, stomach,testicle, uterus, spleen, tongue, brain, thymus, trachea, smallintestine and/or a cancer originating from the non-lymphoid tissue. 86.The method of claim 85, wherein one or more miRNA that is differentiallyexpressed in two or more non-lymphoid tissues is selected from Table 37.87. The method of claim 86, further comprising combining the expressionlevels of two or more miRNAs selected from Table
 37. 88. The method ofclaim 85, wherein one or more miRNA that is differentially expressed intwo or more non-lymhoid tissues is selected from hsa-miR-141,hsa-miR-200a, hsa-miR-200b, hsa-miR-200c, hsa-miR-203, or hsa-miR-429.89. The method of claim 88, further comprising combining the expressionlevels of two or more miRNAs selected from hsa -miR-141, hsa -miR-200a,hsa -miR-200b, hsa -miR-200c, hsa -miR-203, or hsa -miR-429.
 90. A kitcomprising amplification primers and/or hybridization probes for one ormore miRNA selected from Table 4, 5, 6, 10, 11, 12, 13, 14, 16, 17, 18,19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36,37, 39, 40, 41, 43, and/or
 44. 91. The kit of claim 90, comprisingamplification primers and/or hybridization primers for two or more miRNAselected from Table 4, 5, 6, 10, 11, 12, 13, 14, 16, 17, 18, 19, 20, 21,22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 39, 40,41, 43, and/or
 44. 92. The kit of claim 91, comprising amplificationprimers and/or hybridization primers for 5 or more miRNA selected fromTable 4, 5, 6, 10, 11, 12, 13, 14, 16, 17, 18, 19, 20, 21, 22, 23, 24,25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 39, 40, 41, 43,and/or 44.